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WO1999042620A3 - Articles manufactures et procedes de marquage de biomolecules - Google Patents

Articles manufactures et procedes de marquage de biomolecules Download PDF

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Publication number
WO1999042620A3
WO1999042620A3 PCT/US1999/003704 US9903704W WO9942620A3 WO 1999042620 A3 WO1999042620 A3 WO 1999042620A3 US 9903704 W US9903704 W US 9903704W WO 9942620 A3 WO9942620 A3 WO 9942620A3
Authority
WO
WIPO (PCT)
Prior art keywords
manufacture
biomolecules
articles
methods
staining
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1999/003704
Other languages
English (en)
Other versions
WO1999042620A9 (fr
WO1999042620A2 (fr
Inventor
Jack G Chirikjian
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Edvotek
Original Assignee
Edvotek
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Edvotek filed Critical Edvotek
Priority to CA002321100A priority Critical patent/CA2321100A1/fr
Priority to EP99908309A priority patent/EP1057001A2/fr
Priority to JP2000532558A priority patent/JP2002504351A/ja
Priority to AU27771/99A priority patent/AU2777199A/en
Publication of WO1999042620A2 publication Critical patent/WO1999042620A2/fr
Publication of WO1999042620A3 publication Critical patent/WO1999042620A3/fr
Publication of WO1999042620A9 publication Critical patent/WO1999042620A9/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/5436—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand physically entrapped within the solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Paints Or Removers (AREA)

Abstract

Il est possible de marquer ou de colorer des biomolécules qui sont contenues dans des gels ou qui sont situées sur des membranes sans qu'il existe de contact direct avec les solutions toxiques de coloration, pour ce faire, on applique sur le gel ou sur la membrane un article manufacturé comprenant un support souple doté d'un revêtement contenant une coloration ou une marque. Pendant l'application, la coloration ou la marque est transférée du support sur les biomolécules, ceci ayant pour effet de marquer ces dernières. Le volume de solution de coloration utilisé dans cette technique est réduit au maximum, et les dangers pour l'environnement sont per conséquent réduits. De plus, la facilité de la fabrication réduit le coût de l'article.
PCT/US1999/003704 1998-02-19 1999-02-19 Articles manufactures et procedes de marquage de biomolecules Ceased WO1999042620A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002321100A CA2321100A1 (fr) 1998-02-19 1999-02-19 Articles manufactures et procedes de marquage de biomolecules
EP99908309A EP1057001A2 (fr) 1998-02-19 1999-02-19 Articles manufactures et procedes de marquage de biomolecules
JP2000532558A JP2002504351A (ja) 1998-02-19 1999-02-19 生体分子染色用製品及びその方法
AU27771/99A AU2777199A (en) 1998-02-19 1999-02-19 Articles of manufacture and methods for staining biomolecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7517398P 1998-02-19 1998-02-19
US60/075,173 1998-02-19

Publications (3)

Publication Number Publication Date
WO1999042620A2 WO1999042620A2 (fr) 1999-08-26
WO1999042620A3 true WO1999042620A3 (fr) 1999-11-18
WO1999042620A9 WO1999042620A9 (fr) 2000-03-02

Family

ID=22124035

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/003704 Ceased WO1999042620A2 (fr) 1998-02-19 1999-02-19 Articles manufactures et procedes de marquage de biomolecules

Country Status (5)

Country Link
EP (1) EP1057001A2 (fr)
JP (1) JP2002504351A (fr)
AU (1) AU2777199A (fr)
CA (1) CA2321100A1 (fr)
WO (1) WO1999042620A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1988177A1 (fr) 2003-09-30 2008-11-05 Molecular Probes Inc. Détection d'acide nucléique immobilisé
WO2007010532A2 (fr) * 2005-07-18 2007-01-25 Shmuel Bukshpan Compositions destinees a des analyses colorimetriques, et methodes d'utilisation des compositions

Citations (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3678151A (en) * 1969-07-25 1972-07-18 Gugol Clini Tex Inc Biological staining method
DE2739008A1 (de) * 1977-08-30 1979-03-15 Behringwerke Ag Vorrichtung zum nachweis von inhaltsstoffen einer fluessigkeit
US4224277A (en) * 1975-04-11 1980-09-23 Boehringer Mannheim Gmbh Coated slides and apparatus for coating same
EP0119622A2 (fr) * 1983-03-17 1984-09-26 Fuji Photo Film Co., Ltd. Elément intégral pour réaction biologique et procédé pour sa préparation
WO1987003965A1 (fr) * 1985-12-18 1987-07-02 Celldynamics Ag Bandelettes multi-indicatrices pour analyse immunologique, leur utilisation et leur fabrication
EP0246505A2 (fr) * 1986-05-16 1987-11-25 Miles Inc. Procédé de réalisation de bandes d'essai par méthode de coulée
EP0274911A1 (fr) * 1987-01-12 1988-07-20 Pall Corporation Moyen de diagnostic, sa préparation et son utilisation
US4839297A (en) * 1986-11-12 1989-06-13 Boehringer Mannheim Gmbh Test carrier and method for the analytical determination of a component of a body fluid
EP0320842A2 (fr) * 1987-12-18 1989-06-21 W.R. Grace & Co.-Conn. Méthode pour attacher les réactifs biologiques à des supports poreux
WO1990008840A1 (fr) * 1989-02-03 1990-08-09 Eastman Kodak Company Article de test d'un acide nucleique et son utilisation en vue de la detection d'un acide nucleique predetermine
EP0387696A2 (fr) * 1989-03-17 1990-09-19 Abbott Laboratories Procédé et dispositif pour l'hybridisation d'acides nucléiques améliorant la cinétique de la réaction
WO1992007955A1 (fr) * 1990-10-26 1992-05-14 Orion-Yhtymä Oy Procede servant a evaluer si des eprouvettes cliniques conviennent a des analyses d'hybridation et kit servant a effectuer l'evaluation
US5132439A (en) * 1990-11-19 1992-07-21 Promega Corporation Protein staining compositions and methods
WO1993013224A1 (fr) * 1991-12-23 1993-07-08 Chiron Corporation Procede d'immobilisation de sondes d'acide nucleique sur des surfaces de polystyrene
WO1993013405A1 (fr) * 1991-12-23 1993-07-08 Tropix, Inc. Membrane amelioree pour des applications a effet buvard chimioluminescentes
US5316638A (en) * 1989-09-27 1994-05-31 Peter Jackson Treatment of carbohydrates
EP0677589A2 (fr) * 1994-03-31 1995-10-18 Johnson & Johnson Clinical Diagnostics, Inc. Procédé élément de test et trousse d'analyse pour la détection semi-quantitative d'acides nucléiques
US5514501A (en) * 1994-06-07 1996-05-07 The United States Of America As Represented By The Secretary Of Commerce Process for UV-photopatterning of thiolate monolayers self-assembled on gold, silver and other substrates
EP0745689A2 (fr) * 1990-05-11 1996-12-04 Microprobe Corporation Bâtonnet d'hybridation d'acide nucléique
US5585276A (en) * 1991-05-10 1996-12-17 Wisconsin Alumni Research Foundation Medium and method for blotting macromolecules
US5641635A (en) * 1993-11-12 1997-06-24 Eastman Kodak Company Dry elements, test devices, test kits and methods for chemiluminescent detection of analytes using peroxidase-labeled reagents
US5688642A (en) * 1994-12-01 1997-11-18 The United States Of America As Represented By The Secretary Of The Navy Selective attachment of nucleic acid molecules to patterned self-assembled surfaces
US5776684A (en) * 1995-12-28 1998-07-07 Edvotek Method for staining biomolecules using a gelled matrix

Patent Citations (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3678151A (en) * 1969-07-25 1972-07-18 Gugol Clini Tex Inc Biological staining method
US4224277A (en) * 1975-04-11 1980-09-23 Boehringer Mannheim Gmbh Coated slides and apparatus for coating same
DE2739008A1 (de) * 1977-08-30 1979-03-15 Behringwerke Ag Vorrichtung zum nachweis von inhaltsstoffen einer fluessigkeit
EP0119622A2 (fr) * 1983-03-17 1984-09-26 Fuji Photo Film Co., Ltd. Elément intégral pour réaction biologique et procédé pour sa préparation
WO1987003965A1 (fr) * 1985-12-18 1987-07-02 Celldynamics Ag Bandelettes multi-indicatrices pour analyse immunologique, leur utilisation et leur fabrication
EP0246505A2 (fr) * 1986-05-16 1987-11-25 Miles Inc. Procédé de réalisation de bandes d'essai par méthode de coulée
US4839297A (en) * 1986-11-12 1989-06-13 Boehringer Mannheim Gmbh Test carrier and method for the analytical determination of a component of a body fluid
EP0274911A1 (fr) * 1987-01-12 1988-07-20 Pall Corporation Moyen de diagnostic, sa préparation et son utilisation
EP0320842A2 (fr) * 1987-12-18 1989-06-21 W.R. Grace & Co.-Conn. Méthode pour attacher les réactifs biologiques à des supports poreux
WO1990008840A1 (fr) * 1989-02-03 1990-08-09 Eastman Kodak Company Article de test d'un acide nucleique et son utilisation en vue de la detection d'un acide nucleique predetermine
EP0387696A2 (fr) * 1989-03-17 1990-09-19 Abbott Laboratories Procédé et dispositif pour l'hybridisation d'acides nucléiques améliorant la cinétique de la réaction
US5316638A (en) * 1989-09-27 1994-05-31 Peter Jackson Treatment of carbohydrates
EP0745689A2 (fr) * 1990-05-11 1996-12-04 Microprobe Corporation Bâtonnet d'hybridation d'acide nucléique
WO1992007955A1 (fr) * 1990-10-26 1992-05-14 Orion-Yhtymä Oy Procede servant a evaluer si des eprouvettes cliniques conviennent a des analyses d'hybridation et kit servant a effectuer l'evaluation
US5132439A (en) * 1990-11-19 1992-07-21 Promega Corporation Protein staining compositions and methods
US5585276A (en) * 1991-05-10 1996-12-17 Wisconsin Alumni Research Foundation Medium and method for blotting macromolecules
WO1993013224A1 (fr) * 1991-12-23 1993-07-08 Chiron Corporation Procede d'immobilisation de sondes d'acide nucleique sur des surfaces de polystyrene
WO1993013405A1 (fr) * 1991-12-23 1993-07-08 Tropix, Inc. Membrane amelioree pour des applications a effet buvard chimioluminescentes
US5641635A (en) * 1993-11-12 1997-06-24 Eastman Kodak Company Dry elements, test devices, test kits and methods for chemiluminescent detection of analytes using peroxidase-labeled reagents
EP0677589A2 (fr) * 1994-03-31 1995-10-18 Johnson & Johnson Clinical Diagnostics, Inc. Procédé élément de test et trousse d'analyse pour la détection semi-quantitative d'acides nucléiques
US5514501A (en) * 1994-06-07 1996-05-07 The United States Of America As Represented By The Secretary Of Commerce Process for UV-photopatterning of thiolate monolayers self-assembled on gold, silver and other substrates
US5688642A (en) * 1994-12-01 1997-11-18 The United States Of America As Represented By The Secretary Of The Navy Selective attachment of nucleic acid molecules to patterned self-assembled surfaces
US5776684A (en) * 1995-12-28 1998-07-07 Edvotek Method for staining biomolecules using a gelled matrix

Also Published As

Publication number Publication date
EP1057001A2 (fr) 2000-12-06
WO1999042620A9 (fr) 2000-03-02
WO1999042620A2 (fr) 1999-08-26
CA2321100A1 (fr) 1999-08-26
AU2777199A (en) 1999-09-06
JP2002504351A (ja) 2002-02-12

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