[go: up one dir, main page]

WO1998036053A3 - Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages - Google Patents

Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages Download PDF

Info

Publication number
WO1998036053A3
WO1998036053A3 PCT/US1998/002752 US9802752W WO9836053A3 WO 1998036053 A3 WO1998036053 A3 WO 1998036053A3 US 9802752 W US9802752 W US 9802752W WO 9836053 A3 WO9836053 A3 WO 9836053A3
Authority
WO
WIPO (PCT)
Prior art keywords
recombinant dna
dna sequences
purified proteins
processes
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1998/002752
Other languages
French (fr)
Other versions
WO1998036053A2 (en
Inventor
John I Stiles
Istefo Moisyadi
Kabi R Neupane
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Hawaii at Manoa
University of Hawaii at Hilo
Original Assignee
University of Hawaii at Manoa
University of Hawaii at Hilo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Hawaii at Manoa, University of Hawaii at Hilo filed Critical University of Hawaii at Manoa
Priority to PCT/US1998/002752 priority Critical patent/WO1998036053A2/en
Priority to AU63247/98A priority patent/AU6324798A/en
Publication of WO1998036053A2 publication Critical patent/WO1998036053A2/en
Publication of WO1998036053A3 publication Critical patent/WO1998036053A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Tea And Coffee (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Purified proteins, DNA sequences that code on expression therefor and recombinant DNA molecules, including hosts transformed therewith, for transforming coffee plants to suppress the expression of caffeine. The DNA sequences and recombinant DNA molecules are characterized in that they code on expression for an enzyme in the pathway for caffeine synthesis in coffee. Coffee plants transformed with DNA molecules that code on transcription for mRNA that is antisense to mRNA that codes on expression for at least one enzyme in the pathway for caffeine biosynthesis.
PCT/US1998/002752 1998-02-13 1998-02-13 Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages Ceased WO1998036053A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PCT/US1998/002752 WO1998036053A2 (en) 1998-02-13 1998-02-13 Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages
AU63247/98A AU6324798A (en) 1998-02-13 1998-02-13 Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1998/002752 WO1998036053A2 (en) 1998-02-13 1998-02-13 Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages

Publications (2)

Publication Number Publication Date
WO1998036053A2 WO1998036053A2 (en) 1998-08-20
WO1998036053A3 true WO1998036053A3 (en) 1999-12-09

Family

ID=22266381

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/002752 Ceased WO1998036053A2 (en) 1998-02-13 1998-02-13 Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages

Country Status (2)

Country Link
AU (1) AU6324798A (en)
WO (1) WO1998036053A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6075184A (en) * 1996-03-26 2000-06-13 University Of Hawaii Purified proteins, recombinant DNA sequences and processes for producing caffeine free beverages
US6930227B1 (en) * 1999-05-26 2005-08-16 Mitsui Chemicals, Inc. Camellia sinensis gene encoding a caffeine synthesis associated n-methyl transferase with 7-methylxanthine n3 methyl transferase, theobromine n1 methyl transferase, and paraxanthine n3 methyl transferase activities and use thereof
EP1256629A1 (en) * 2001-05-11 2002-11-13 Société des Produits Nestlé S.A. Leaf specifc gene promoter of coffee
DK3684930T3 (en) 2017-09-19 2022-06-07 Tropic Biosciences Uk Ltd Modification of the specificity of non-coding plant RNA molecules for inactivation of gene expression
GB201807192D0 (en) 2018-05-01 2018-06-13 Tropic Biosciences Uk Ltd Compositions and methods for reducing caffeine content in coffee beans
IL305071A (en) * 2021-03-02 2023-10-01 Betterseeds Ltd Domestication of leguminous plants

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5334529A (en) * 1989-06-27 1994-08-02 Escagenetics Corporation Stably transformed coffee plant cells and plantlets
WO1997035960A1 (en) * 1996-03-26 1997-10-02 University Of Hawaii Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5334529A (en) * 1989-06-27 1994-08-02 Escagenetics Corporation Stably transformed coffee plant cells and plantlets
WO1997035960A1 (en) * 1996-03-26 1997-10-02 University Of Hawaii Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KATO M ET AL: "CAFFEINE BIOSYNTHESIS IN YOUNG LEAVES OF CAMELLIA SINENSIS: IN VITRO STUDIES ON NU-METHYLTRANSFERASE ACTIVITY INVOLVED IN THE CONVERSION OF XANTHOSINE TO CAFFEINE", PHYSIOLOGIA PLANTARUM, vol. 98, no. 3, 1 January 1996 (1996-01-01), pages 629 - 636, XP002071639, ISSN: 0031-9317 *
SCHULTHESS B H ET AL: "CAFFEINE BIOSYNTHESIS STARTS WITH THE METABOLICALLY CHANNELLED FORMATION OF 7-METHYL-XMP-A NEW HYPOTHESIS", PHYTOCHEMISTRY, vol. 41, no. 1, 1 January 1996 (1996-01-01), pages 169 - 175, XP002071640, ISSN: 0031-9422 *
SUZUKI T ET AL: "PURINE AND PURINE ALKALOID METABOLISM IN CAMELLIA AND COFFEA PLANTS", PHYTOCHEMISTRY, vol. 31, no. 8, 1 January 1992 (1992-01-01), pages 2575 - 2584, XP002071641, ISSN: 0031-9422 *

Also Published As

Publication number Publication date
AU6324798A (en) 1998-09-08
WO1998036053A2 (en) 1998-08-20

Similar Documents

Publication Publication Date Title
WO1997035960A8 (en) Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages
WO2003046173A1 (en) siRNA EXPRESSION SYSTEM AND PROCESS FOR PRODUCING FUNCTIONAL GENE-KNOCKDOWN CELLS AND THE LIKE USING THE SAME
EP1568774A3 (en) Recombinant production of novel polyketides
AU4077100A (en) Enhancement of the immune response for vaccine and gene therapy applications
WO2002046381A3 (en) Method for the prevention or reduction of haze in beverages
WO2000008158A3 (en) Molecules designated ldcam
EP1224266A4 (en) Lysosomal targeting pathway enzymes
WO2004044140A3 (en) 2’-substituted oligomeric compounds and compositions for use in gene modulations
AU3246300A (en) Polypeptides having galactose oxidase activity and nucleic acids encoding same
IL140938A0 (en) Molecules designated b7l-1
WO1999055888A3 (en) Carotenoid biosynthesis enzymes
WO1995027790A3 (en) Dna sequences coding for a cynnamoyl coa reductase and their use in the regulation of plant lignin concentrations
DE69634361D1 (en) DNA Sequencing Encoding Cinnamoyl-CoA Reductase, And Its Use To Regulate Lignune Content Of Plants
WO2003004628A3 (en) Method for purifying an enzyme, a purified enzyme produced thereby, and use of this enzyme
WO1998036053A3 (en) Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages
WO2002060917A3 (en) Method to treat hemophilia
AP9901461A0 (en) Purified proteins, recombinant DNA sequences and process for controlling the ripening of coffee plants.
DE69734828D1 (en) 5-AMINOLEVULINSEAU SYNTHASE FROM ASPERGILLUS ORYZAE AND DAFUER CODING NUCLEIC ACID
EP0647280A4 (en) OVEREXPRESSION OF SINGLE STRANDED MOLECULES.
WO1999053069A3 (en) Cell cycle regulatory proteins cdc2 and pitslre from plants
WO1999047689A3 (en) Inhibitors of apoptosis proteins in plants
WO2000036082A3 (en) NOVEL FUNGAL mRNA CAPPING ENZYMES, AND METHODS OF USING THE SAME
EP1097999A3 (en) Abscisic acid responsive element-binding transcription factors
WO2001049840A3 (en) Spf1-related transcription factors
AU2002306860A1 (en) Isolated human nadph oxidase, nucleic acid molecules encoding said proteins, and uses thereof

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH HU IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: CA

AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH HU IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN ML MR NE SN TD TG

122 Ep: pct application non-entry in european phase