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WO1998032847A2 - Caracterisation du transcriptome de levure - Google Patents

Caracterisation du transcriptome de levure Download PDF

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Publication number
WO1998032847A2
WO1998032847A2 PCT/US1998/001216 US9801216W WO9832847A2 WO 1998032847 A2 WO1998032847 A2 WO 1998032847A2 US 9801216 W US9801216 W US 9801216W WO 9832847 A2 WO9832847 A2 WO 9832847A2
Authority
WO
WIPO (PCT)
Prior art keywords
gene
group
norf
phase
yeast
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1998/001216
Other languages
English (en)
Other versions
WO1998032847A3 (fr
Inventor
Victor E. Velculescu
Bert Vogelstein
Kenneth W. Kinzler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Johns Hopkins University
Original Assignee
Johns Hopkins University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johns Hopkins University filed Critical Johns Hopkins University
Priority to CA002278645A priority Critical patent/CA2278645A1/fr
Priority to EP98902680A priority patent/EP0970202A2/fr
Priority to AU59280/98A priority patent/AU749606C/en
Priority to JP53211798A priority patent/JP2001509017A/ja
Publication of WO1998032847A2 publication Critical patent/WO1998032847A2/fr
Publication of WO1998032847A3 publication Critical patent/WO1998032847A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces

Definitions

  • Locus and Description denote the locus name, and functional description of each ORF, respectively; Copies/cell represents the abundance of each transcript in the SAGE library, assuming 15,000 total transcripts per cell and 60,633 ascertained transcripts.
  • ORF Nonannotated ORF
  • nocodazole 15ug/ml was added to early log phase cells and the culture was incubated for an additional 100 minutes at 30°C.
  • Harvested cells were washed once with water prior to freezing at -70 °C.
  • the growth states of the harvested cells were confirmed by microscopic and flow cytometric analyses (Basrai et al., 1996).
  • GAL3 carbon regulation; UASGal elements in common with GAL1, GAL2, GAL7, GAL 10, GAL80, and MEL1; encoded protein strikingly similar to yeast and Escherichia coli galactokinases. Mol Cell Biol 8, 3439-3447.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Mycology (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Cette invention concerne des gènes de levure qui sont exprimés de manière différentielle au cours du cycle cellulaire. On peut les utiliser pour étudier, modifier et surveiller le cycle cellulaire d'une cellule eucaryote; pour obtenir des homologues humains impliqués dans la régulation du cycle cellulaire, et pour identifier des agents antifongiques. On peut les former sous forme de réseaux sur des supports solides pour étudier un transcriptome de cellule dans diverses conditions.
PCT/US1998/001216 1997-01-23 1998-01-22 Caracterisation du transcriptome de levure Ceased WO1998032847A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002278645A CA2278645A1 (fr) 1997-01-23 1998-01-22 Caracterisation du transcriptome de levure
EP98902680A EP0970202A2 (fr) 1997-01-23 1998-01-22 Caracterisation du transcriptome de levure
AU59280/98A AU749606C (en) 1997-01-23 1998-01-22 Characterization of the yeast transcriptome
JP53211798A JP2001509017A (ja) 1997-01-23 1998-01-22 イーストトランスクリプトームのキャラクタリゼーション

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3591797P 1997-01-23 1997-01-23
US60/035,917 1997-01-23

Publications (2)

Publication Number Publication Date
WO1998032847A2 true WO1998032847A2 (fr) 1998-07-30
WO1998032847A3 WO1998032847A3 (fr) 1998-11-26

Family

ID=21885540

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/001216 Ceased WO1998032847A2 (fr) 1997-01-23 1998-01-22 Caracterisation du transcriptome de levure

Country Status (5)

Country Link
EP (1) EP0970202A2 (fr)
JP (1) JP2001509017A (fr)
AU (1) AU749606C (fr)
CA (1) CA2278645A1 (fr)
WO (1) WO1998032847A2 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000077214A3 (fr) * 1999-06-16 2001-05-03 Univ Johns Hopkins Caracterisation du transcriptome d'une levure
FR2821087A1 (fr) * 2001-02-16 2002-08-23 Centre Nat Rech Scient Procede d'analyse qualitative et quantitative d'une population d'acides nucleiques contenus dans un echantillon
US7270977B2 (en) 2001-12-11 2007-09-18 Bayer Cropscience Ag Polypeptides for identifying fungicidally active compounds
US7504493B2 (en) 1997-01-23 2009-03-17 The John Hopkins University Characterization of the yeast transcriptome
US8283158B2 (en) 2002-11-25 2012-10-09 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Method and apparatus for performing multiple simultaneous manipulations of biomolecules in a two dimensional array

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5143552B2 (ja) * 2004-07-23 2013-02-13 ジーイー・ヘルスケア・ユーケイ・リミテッド 細胞周期フェーズマーカー
JP2018531610A (ja) * 2015-11-02 2018-11-01 オリジェン， インコーポレイテッド 細胞周期ブロックは、人工多能性幹細胞の生成効率を改善する

Non-Patent Citations (17)

* Cited by examiner, † Cited by third party
Title
A. GOFFEAU ET AL.: "Life with 6000 genes" SCIENCE, vol. 274, 25 October 1996, AAAS,WASHINGTON,DC,US, pages 546-567, XP002070912 cited in the application *
BISHOP D K ET AL: "DMC1: A MEIOSIS-SPECIFIC YEAST HOMOLOG OF E. COLI RECA REQUIRED FORRECOMBINATION, SYNAPTONEMAL COMPLEX FORMATION, AND CELL CYCLE PROGRESSION" CELL, vol. 69, 1 May 1992, pages 439-456, XP000676406 *
BURNS N ET AL: "LARGE-SCALE ANALYSIS OF GENE EXPRESSION, PROTEIN LOCALIZATION, AND GENE DISRUPTION IN SACCHAROMYCES CERECISIAE" GENES AND DEVELOPMENT, vol. 8, no. 9, 1 May 1994, pages 1087-1105, XP000569086 *
C. FRANKHAUSER ET AL.: "The S. pombe cdc15 gene is a key element in the reorganization of F-actin at mitose" CELL, vol. 82, 11 August 1995, CELL PRESS,CAMBRIDGE,MA,US;, pages 435-444, XP002070907 *
C. PRICE ET AL.: "A general approach to the isolation of cell cycle-regulated genes in the budding yeast, Saccharomyces cerevisiae" J. MOL. BIOL., vol. 218, no. 3, 5 April 1991, ACADEMIC PRESS,US, pages 543-556, XP002070908 *
J.H. TOYN ET AL.: "The cell-cycle-regulated yeast gene DBF2, encoding a putative protein kinase, has a homologue that is not under cell-cycle control" GENE, vol. 104, 1991, ELSEVIER SCIENCE PUBLISHERS,B.V.,AMSTERDAM,NL;, pages 63-70, XP002070905 *
K. NASMYTH: "Control of the yeast cell cycle by the Cdc28 protein kinase" CURRENT OPINION IN CELL BIOLOGY, vol. 5, no. 2, April 1993, CURRENT BIOLOGY, LTD., US, pages 166-179, XP002071095 *
LOCKHART D J ET AL: "EXPRESSION MONITORING BY HYBRIDIZATION TO HIGH-DENSITY OLIGONUCLEOTIDE ARRAYS" BIO/TECHNOLOGY, vol. 14, no. 13, December 1996, pages 1675-1680, XP002022521 cited in the application *
M. SCHENA ET AL.: "Paralel human genome analysis: Microarray-based expression monitoring of 1000 genes" PROC. NATL. ACAD. SCI., vol. 93, October 1996, NATL. ACAD. SCI.,WASHINGTON,DC,US;, pages 10614-10619, XP002070911 *
R.M. GOLDSTEYN ET AL.: "Cell cycle analysis and chromosomal localization of human Plk1, a putative homologue of the mitotic kinases Drosophila polo and Saccharomyces cerevisiae Cdc5" J. CELL SCIENCE, vol. 107, no. 6, 1994, THE COMPANY OF BIOLOGISTS LIMITED, GB, pages 1509-1517, XP002070910 *
S.J. ELLEDGE AND R.W. DAVIS: "DNA damage induction of ribonucleotide reductase" MOL. CELL. BIOL., vol. 9, no. 11, November 1989, ASM WASHINGTON, DC,US, pages 4932-4940, XP002070906 cited in the application *
SCHENA M ET AL: "QUANTITATIVE MONITORING OF GENE EXPRESSION PATTERNS WITH A COMPLEMENTARY DNA MICROARRAY" SCIENCE, vol. 270, no. 5235, 20 October 1995, pages 467-470, XP000644675 cited in the application *
SHALON D ET AL: "A DNA MICROARRAY SYSTEM FOR ANALYZING COMPLEX DNA SAMPLES USING TWO-COLOR FLUORESCENT PROBE HYBRIDIZATION" GENOME RESEARCH, vol. 6, no. 7, 1 July 1996, pages 639-645, XP000597095 *
T. YASUGI AND P.M. HOWLEY: "Identification of the structural and functional human homolog of the yeast ubiquitin conjugated enzyme UBC9" NUCLEIC ACIDS RESEARCH, vol. 24, no. 11, 1996, IRL PRESS LIMITED,OXFORD,ENGLAND, pages 2005-2010, XP002070909 cited in the application *
V.E.VCELCULESCU ET AL.: "Characterization of the yeast transcriptome" CELL, vol. 88, 24 January 1997, CELL PRESS,CAMBRIDGE,MA,US;, pages 243-251, XP002070903 *
VELCULESCU V E ET AL: "SERIAL ANALYSIS OF GENE EXPRESSION" SCIENCE, vol. 270, 20 October 1995, pages 484-487, XP002053721 cited in the application *
Y. KYU JANG ET AL.: "Differential expression of the rhp51+ gene, a recA and RAD51 homolog from the fission yeast Schizosaccharomyces pombe" GENE, vol. 169, 1996, ELSEVIER SCIENCE PUBLISHERS,B.V.,AMSTERDAM,NL;, pages 125-130, XP002070904 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7504493B2 (en) 1997-01-23 2009-03-17 The John Hopkins University Characterization of the yeast transcriptome
WO2000077214A3 (fr) * 1999-06-16 2001-05-03 Univ Johns Hopkins Caracterisation du transcriptome d'une levure
FR2821087A1 (fr) * 2001-02-16 2002-08-23 Centre Nat Rech Scient Procede d'analyse qualitative et quantitative d'une population d'acides nucleiques contenus dans un echantillon
WO2002066676A3 (fr) * 2001-02-16 2002-10-17 Centre Nat Rech Scient Procede d'analyse qualitative et quantitative d'une population d'acides nucleiques contenus dans un echantillon
US7270977B2 (en) 2001-12-11 2007-09-18 Bayer Cropscience Ag Polypeptides for identifying fungicidally active compounds
US8283158B2 (en) 2002-11-25 2012-10-09 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Method and apparatus for performing multiple simultaneous manipulations of biomolecules in a two dimensional array

Also Published As

Publication number Publication date
JP2001509017A (ja) 2001-07-10
AU749606C (en) 2007-05-17
AU5928098A (en) 1998-08-18
EP0970202A2 (fr) 2000-01-12
AU749606B2 (en) 2002-06-27
CA2278645A1 (fr) 1998-07-30
WO1998032847A3 (fr) 1998-11-26

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