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WO1998013527A3 - Compositions and methods for enhancing hybridization specificity - Google Patents

Compositions and methods for enhancing hybridization specificity Download PDF

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Publication number
WO1998013527A3
WO1998013527A3 PCT/US1997/017413 US9717413W WO9813527A3 WO 1998013527 A3 WO1998013527 A3 WO 1998013527A3 US 9717413 W US9717413 W US 9717413W WO 9813527 A3 WO9813527 A3 WO 9813527A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
compositions
specificity
nucleic acid
organic solvents
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1997/017413
Other languages
French (fr)
Other versions
WO1998013527A2 (en
Inventor
Ness Jeffrey Van
John Tabone
Lori K Garrison
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UCB Manufacturing Inc
Original Assignee
Darwin Molecular Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Darwin Molecular Corp filed Critical Darwin Molecular Corp
Priority to EP99107983A priority Critical patent/EP0952228A3/en
Priority to EP97944521A priority patent/EP0958378A2/en
Priority to JP51598298A priority patent/JP2002514909A/en
Priority to AU45997/97A priority patent/AU4599797A/en
Priority to CA002266847A priority patent/CA2266847A1/en
Publication of WO1998013527A2 publication Critical patent/WO1998013527A2/en
Publication of WO1998013527A3 publication Critical patent/WO1998013527A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Compositions and methods are provided for increasing the specificity of a probe nucleic acid for a target nucleic acid in a hybridization solution. An abasic residue, deoxyNebularine residue, or a hybotrope is used to increase specificity. A method is provided for identifying useful hybotropes, including salts, water miscible organic solvents, aprotic solvents and organic solvents, on the basis of enthalpy considerations. Hybotropic hybridization and modified oligonucleotides may be used in amplification reactions, such as PCR, sequence analysis methods, and genomic screening methods.
PCT/US1997/017413 1996-09-24 1997-09-24 Compositions and methods for enhancing hybridization specificity Ceased WO1998013527A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP99107983A EP0952228A3 (en) 1996-09-24 1997-09-24 Compositions and methods for enhancing hybridization specificity
EP97944521A EP0958378A2 (en) 1996-09-24 1997-09-24 Compositions and methods for enhancing hybridization specificity
JP51598298A JP2002514909A (en) 1996-09-24 1997-09-24 Compositions and methods for enhancing hybridization specificity
AU45997/97A AU4599797A (en) 1996-09-24 1997-09-24 Compositions and methods for enhancing hybridization specificity
CA002266847A CA2266847A1 (en) 1996-09-24 1997-09-24 Compositions and methods for enhancing hybridization specificity

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US71913296A 1996-09-24 1996-09-24
US2662196P 1996-09-24 1996-09-24
US08/719,132 1996-09-24
US60/026,621 1996-09-24

Publications (2)

Publication Number Publication Date
WO1998013527A2 WO1998013527A2 (en) 1998-04-02
WO1998013527A3 true WO1998013527A3 (en) 1998-08-20

Family

ID=26701464

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/017413 Ceased WO1998013527A2 (en) 1996-09-24 1997-09-24 Compositions and methods for enhancing hybridization specificity

Country Status (5)

Country Link
EP (1) EP0958378A2 (en)
JP (1) JP2002514909A (en)
AU (1) AU4599797A (en)
CA (1) CA2266847A1 (en)
WO (1) WO1998013527A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6872521B1 (en) * 1998-06-16 2005-03-29 Beckman Coulter, Inc. Polymerase signaling assay
WO2001023618A2 (en) * 1999-09-30 2001-04-05 Qiagen Genomics, Inc. Compositions and methods for reducing oligonucleotide hybridization and priming specificity
JP2004520812A (en) * 2000-08-30 2004-07-15 ハプロゲン・エルエルシー Methods for determining alleles
WO2002074988A2 (en) 2001-03-16 2002-09-26 The Chancellor, Master And Scholars Of The University Of Oxford Arrays and methods of use
US6753145B2 (en) * 2001-07-05 2004-06-22 Agilent Technologies, Inc. Buffer composition and method for hybridization of microarrays on adsorbed polymer siliceous surfaces
CA2524572C (en) * 2003-05-01 2018-07-10 Gen-Probe Incorporated Oligonucleotides comprising a molecular switch having enhanced sensitivity to the presence of a mismatch
CA2494571C (en) * 2003-12-02 2010-02-09 F.Hoffmann-La Roche Ag Oligonucleotides containing molecular rods
KR100601986B1 (en) * 2005-02-02 2006-07-18 삼성전자주식회사 How to hybridize genes
KR101110013B1 (en) * 2007-10-05 2012-02-29 (주)바이오니아 Primers for pcr amplification comprising abasic parts within the primer sequences
US9944975B2 (en) 2015-09-03 2018-04-17 Abbott Molecular Inc. Hybridization buffers
EP3400307A1 (en) 2016-01-08 2018-11-14 Abbott Molecular Inc. Hybridization buffers comprising guanidinium thiocyanate
KR102750775B1 (en) * 2021-09-13 2025-01-09 건국대학교 산학협력단 Method of salt mediated immobilization of nucleic acids, the solid support including nucleic acids immobilized thereby the method, lateral flow assay strips including the solid support and method of detection of target nucleic acid

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987006621A1 (en) * 1986-05-02 1987-11-05 David Gillespie Chaotropic method for evaluating nucleic acids in a biological sample
WO1990012116A1 (en) * 1989-04-05 1990-10-18 Gene-Trak Systems Hybridization promotion reagents
EP0420260A2 (en) * 1989-09-29 1991-04-03 F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft Biotin-labelled DNA by polymerase chain reaction and detection thereof
WO1992015708A1 (en) * 1991-02-27 1992-09-17 Amoco Corporation Methods for improving the sensitivity of hybridization assays
WO1992018649A1 (en) * 1991-04-12 1992-10-29 Microprobe Corporation Compositions and methods for improved extraction and hybridization of nucleic acid
WO1993020234A1 (en) * 1992-03-31 1993-10-14 E.I. Du Pont De Nemours And Company A rapid, high capacity nucleic acid based assay
WO1994006815A1 (en) * 1992-09-11 1994-03-31 Isis Pharmaceuticals, Inc. Oligonucleotide and nucleotide amine analogs, methods of synthesis and use
EP0628571A1 (en) * 1992-09-07 1994-12-14 Nippon Steel Corporation Novel peptide, and antithrombotic agent, anticoagulant for extracorporeal circulation, cell fusion inhibitor, cancer metastasis inhibitor, protective for platelet preparation for transfusion and pack of platelet preparation for transfusion
WO1995019776A1 (en) * 1994-01-19 1995-07-27 The Trustees Of Columiba University In The City Of New York A method of treating glaucoma
WO1995030774A1 (en) * 1994-05-05 1995-11-16 Beckman Instruments, Inc. Oligonucleotide repeat arrays

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987006621A1 (en) * 1986-05-02 1987-11-05 David Gillespie Chaotropic method for evaluating nucleic acids in a biological sample
WO1990012116A1 (en) * 1989-04-05 1990-10-18 Gene-Trak Systems Hybridization promotion reagents
EP0420260A2 (en) * 1989-09-29 1991-04-03 F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft Biotin-labelled DNA by polymerase chain reaction and detection thereof
WO1992015708A1 (en) * 1991-02-27 1992-09-17 Amoco Corporation Methods for improving the sensitivity of hybridization assays
WO1992018649A1 (en) * 1991-04-12 1992-10-29 Microprobe Corporation Compositions and methods for improved extraction and hybridization of nucleic acid
WO1993020234A1 (en) * 1992-03-31 1993-10-14 E.I. Du Pont De Nemours And Company A rapid, high capacity nucleic acid based assay
EP0628571A1 (en) * 1992-09-07 1994-12-14 Nippon Steel Corporation Novel peptide, and antithrombotic agent, anticoagulant for extracorporeal circulation, cell fusion inhibitor, cancer metastasis inhibitor, protective for platelet preparation for transfusion and pack of platelet preparation for transfusion
WO1994006815A1 (en) * 1992-09-11 1994-03-31 Isis Pharmaceuticals, Inc. Oligonucleotide and nucleotide amine analogs, methods of synthesis and use
WO1995019776A1 (en) * 1994-01-19 1995-07-27 The Trustees Of Columiba University In The City Of New York A method of treating glaucoma
WO1995030774A1 (en) * 1994-05-05 1995-11-16 Beckman Instruments, Inc. Oligonucleotide repeat arrays

Also Published As

Publication number Publication date
WO1998013527A2 (en) 1998-04-02
AU4599797A (en) 1998-04-17
CA2266847A1 (en) 1998-04-02
JP2002514909A (en) 2002-05-21
EP0958378A2 (en) 1999-11-24

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