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WO1998005675A3 - Radiolabeled o-methyl phosphotriester and o-methyl phosphorothioate internucleoside linkages and oligonucleotides containing the same - Google Patents

Radiolabeled o-methyl phosphotriester and o-methyl phosphorothioate internucleoside linkages and oligonucleotides containing the same Download PDF

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Publication number
WO1998005675A3
WO1998005675A3 PCT/US1997/013554 US9713554W WO9805675A3 WO 1998005675 A3 WO1998005675 A3 WO 1998005675A3 US 9713554 W US9713554 W US 9713554W WO 9805675 A3 WO9805675 A3 WO 9805675A3
Authority
WO
WIPO (PCT)
Prior art keywords
methyl
radiolabeled
same
oligonucleotides containing
internucleoside linkages
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1997/013554
Other languages
French (fr)
Other versions
WO1998005675A2 (en
Inventor
Radhakrishnan P Iyer
Theresa Devlin
Ivan Habus
Dong Yu
Sudhir Agrawal
Suzanne M Johnson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aceragen Inc
Original Assignee
Hybridon Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hybridon Inc filed Critical Hybridon Inc
Priority to AU40493/97A priority Critical patent/AU4049397A/en
Publication of WO1998005675A2 publication Critical patent/WO1998005675A2/en
Publication of WO1998005675A3 publication Critical patent/WO1998005675A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention provides oligonucleotides containing methyl phosphotriester linkages and processes for making and methods for using such oligonucleotides. The preparation of radiolabeled oligonucleotides is also disclosed. The radiolabel is a 14C-methyl group attached to an oxygen atom of an internucleoside phosphate group.
PCT/US1997/013554 1996-08-05 1997-08-01 Radiolabeled o-methyl phosphotriester and o-methyl phosphorothioate internucleoside linkages and oligonucleotides containing the same Ceased WO1998005675A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU40493/97A AU4049397A (en) 1996-08-05 1997-08-01 Radiolabeled o-methyl phosphotriester and o-methyl phosphorothioate internucleoside linkages and oligonucleotides containing the same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US68919296A 1996-08-05 1996-08-05
US08/689,192 1996-08-05

Publications (2)

Publication Number Publication Date
WO1998005675A2 WO1998005675A2 (en) 1998-02-12
WO1998005675A3 true WO1998005675A3 (en) 1998-04-02

Family

ID=24767413

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/013554 Ceased WO1998005675A2 (en) 1996-08-05 1997-08-01 Radiolabeled o-methyl phosphotriester and o-methyl phosphorothioate internucleoside linkages and oligonucleotides containing the same

Country Status (2)

Country Link
AU (1) AU4049397A (en)
WO (1) WO1998005675A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8785409B2 (en) 2007-01-30 2014-07-22 Geron Corporation Compounds having anti-adhesive effects on cancer cells

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
H.M. SASMOR ET AL.: "A Practical Method for the Synthesis and Purification of 14C Labeled Oligonucleotides", J. LABELED COMPD. RADIOPHARM., vol. 36, 1995, pages 15 - 31, XP002046254 *
N.T. THUONG AND M. CHASSIGNOL: "Synthése et réactivité d'oligothymidylates substitués par un agent intercalant et un groupe thiophosphate", TETRAHEDR. LETT., vol. 28, 1987, pages 4157 - 60, XP002046252 *
W. TAN ET AL.: "An Efficient Synthesis of Radioisotopically Labeled Oligonucleotides through Direct Solid-Phase 5'-Phosphitylation", TETRAHEDR. LETT., vol. 36, 1995, pages 5323 - 6, XP002046253 *

Also Published As

Publication number Publication date
AU4049397A (en) 1998-02-25
WO1998005675A2 (en) 1998-02-12

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