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WO1997039130A3 - Method for expressing and secreting keratinase - Google Patents

Method for expressing and secreting keratinase Download PDF

Info

Publication number
WO1997039130A3
WO1997039130A3 PCT/US1997/006477 US9706477W WO9739130A3 WO 1997039130 A3 WO1997039130 A3 WO 1997039130A3 US 9706477 W US9706477 W US 9706477W WO 9739130 A3 WO9739130 A3 WO 9739130A3
Authority
WO
WIPO (PCT)
Prior art keywords
keratinase
host cell
secreting
expressing
keratinase enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1997/006477
Other languages
French (fr)
Other versions
WO1997039130A2 (en
Inventor
Jason C H Shih
Xiang Lin
Sui-Lam Wong
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University Technologies International Inc
University of North Carolina at Chapel Hill
North Carolina State University
Original Assignee
University Technologies International Inc
University of North Carolina at Chapel Hill
North Carolina State University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Technologies International Inc, University of North Carolina at Chapel Hill, North Carolina State University filed Critical University Technologies International Inc
Priority to AU31144/97A priority Critical patent/AU3114497A/en
Publication of WO1997039130A2 publication Critical patent/WO1997039130A2/en
Publication of WO1997039130A3 publication Critical patent/WO1997039130A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention provides a Bacillus subtilis host cell capable of expressing and secreting keratinase. The host cell contains a recombinant DNA molecule comprising vector DNA and DNA encoding Bacillus licheniformis PWD-1 keratinase enzyme operatively associated therewith. The present invention also provides a method for producing keratinase enzyme. The method includes the steps of (a) culturing a Bacillus subtilis host cell containing a recombinant DNA molecule comprising vector DNA and DNA encoding Bacillus licheniformis PWD-1 keratinase enzyme operatively associated therewith, and (b) collecting keratinase enzyme from the cell culture.
PCT/US1997/006477 1996-04-18 1997-04-16 Method for expressing and secreting keratinase Ceased WO1997039130A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU31144/97A AU3114497A (en) 1996-04-18 1997-04-16 Method for expressing and secreting keratinase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US63444096A 1996-04-18 1996-04-18
US08/634,440 1996-04-18

Publications (2)

Publication Number Publication Date
WO1997039130A2 WO1997039130A2 (en) 1997-10-23
WO1997039130A3 true WO1997039130A3 (en) 1998-02-12

Family

ID=24543801

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/006477 Ceased WO1997039130A2 (en) 1996-04-18 1997-04-16 Method for expressing and secreting keratinase

Country Status (3)

Country Link
AU (1) AU3114497A (en)
WO (1) WO1997039130A2 (en)
ZA (1) ZA973310B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2493722C (en) 2002-07-29 2011-11-01 Aminotech As Method for recovering peptides/amino acids and oil/fat from one or more protein-containing raw materials, products produced by the method, and use of the products
US7915024B2 (en) 2002-08-09 2011-03-29 North Carolina State University Methods and compositions for improving growth of meat-type poultry
MXPA05002823A (en) * 2002-09-13 2005-05-27 Univ North Carolina State Construction of bacillus licheniformis.

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0310137A2 (en) * 1987-10-02 1989-04-05 Zymogenetics, Inc. BAR1 secretion signal
WO1989009278A1 (en) * 1988-03-31 1989-10-05 North Carolina State University Method of degrading keratinaceous material and bacteria useful therefor
WO1995033056A1 (en) * 1994-05-27 1995-12-07 North Carolina State University Dna encoding the bacillus licheniformis pwd-1 keratinase

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0310137A2 (en) * 1987-10-02 1989-04-05 Zymogenetics, Inc. BAR1 secretion signal
WO1989009278A1 (en) * 1988-03-31 1989-10-05 North Carolina State University Method of degrading keratinaceous material and bacteria useful therefor
WO1995033056A1 (en) * 1994-05-27 1995-12-07 North Carolina State University Dna encoding the bacillus licheniformis pwd-1 keratinase

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
LIN X ET AL: "Gene sequence and expression of keratinase in the feather-degrading Bacillus licheniformis PWD -1.", SIXTEENTH ANNUAL MEETING OF THE SOUTHERN POULTRY SCIENCE SOCIETY, ATLANTA, GEORGIA, USA, JANUARY 18-20, 1995. POULTRY SCIENCE 74 (SUPPL. 1). 1995. 201. ISSN: 0032-5791, XP002042753 *
PEI-ZHI WANG ET AL.: "Overlapping promoters transcribed by Bacillus subtilis sigma55 and sigma37 RNA polymerase holoenzymes during growth and stationary phases", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 259, no. 13, 10 July 1984 (1984-07-10), MD US, pages 8619 - 8625, XP002042749 *
XIANG LIN ET AL.: "Nucleotide sequence and expression of kerA, the gene encoding a keratinolytic protease of Bacillus licheniformis PWD-1", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 61, no. 4, April 1995 (1995-04-01), pages 1469 - 1474, XP002042752 *

Also Published As

Publication number Publication date
WO1997039130A2 (en) 1997-10-23
AU3114497A (en) 1997-11-07
ZA973310B (en) 1998-07-30

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