[go: up one dir, main page]

WO1997037039A3 - Verwendung extrem thermophiler dna-polymerasen - Google Patents

Verwendung extrem thermophiler dna-polymerasen Download PDF

Info

Publication number
WO1997037039A3
WO1997037039A3 PCT/EP1997/001589 EP9701589W WO9737039A3 WO 1997037039 A3 WO1997037039 A3 WO 1997037039A3 EP 9701589 W EP9701589 W EP 9701589W WO 9737039 A3 WO9737039 A3 WO 9737039A3
Authority
WO
WIPO (PCT)
Prior art keywords
polymerases
extremely thermophilic
thermophilic dna
dna
extremely
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP1997/001589
Other languages
English (en)
French (fr)
Other versions
WO1997037039A2 (de
Inventor
Juergen Blusch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Helmholtz Zentrum Muenchen Deutsches Forschungszentrum fuer Gesundheit und Umwelt GmbH
Original Assignee
Helmholtz Zentrum Muenchen Deutsches Forschungszentrum fuer Gesundheit und Umwelt GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Helmholtz Zentrum Muenchen Deutsches Forschungszentrum fuer Gesundheit und Umwelt GmbH filed Critical Helmholtz Zentrum Muenchen Deutsches Forschungszentrum fuer Gesundheit und Umwelt GmbH
Priority to EP97915455A priority Critical patent/EP0904404A2/de
Priority to JP53492797A priority patent/JP3378255B2/ja
Priority to US09/155,512 priority patent/US6251637B1/en
Publication of WO1997037039A2 publication Critical patent/WO1997037039A2/de
Publication of WO1997037039A3 publication Critical patent/WO1997037039A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Erfindungsgemäß wird ein Verfahren zur Zyklus-Sequenzierung von DNA mit Dye-Terminatoren offenbart, wobei extrem thermophile DNA-Polymerasen bei einer Kettenverlängerungstemperatur im Bereich von > 60 °C bis 80 °C verwendet werden. Weiterhin wird eine Verwendung extrem thermophiler DNA-Polymerasen bei Temperatur im Bereich von > 60 °C bis 80 °C offenbart.
PCT/EP1997/001589 1996-03-29 1997-03-27 Verwendung extrem thermophiler dna-polymerasen Ceased WO1997037039A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP97915455A EP0904404A2 (de) 1996-03-29 1997-03-27 Verwendung extrem thermophiler dna-polymerasen
JP53492797A JP3378255B2 (ja) 1996-03-29 1997-03-27 超好熱性ｄｎａポリメラーゼの使用
US09/155,512 US6251637B1 (en) 1996-03-29 1997-03-27 Use of extremely thermophilic DNA-polymerases

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19612684.3 1996-03-29
DE19612684A DE19612684A1 (de) 1996-03-29 1996-03-29 Neue Verwendung extrem thermophiler DNA-Polymerasen

Publications (2)

Publication Number Publication Date
WO1997037039A2 WO1997037039A2 (de) 1997-10-09
WO1997037039A3 true WO1997037039A3 (de) 1997-11-27

Family

ID=7789948

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1997/001589 Ceased WO1997037039A2 (de) 1996-03-29 1997-03-27 Verwendung extrem thermophiler dna-polymerasen

Country Status (5)

Country Link
US (1) US6251637B1 (de)
EP (1) EP0904404A2 (de)
JP (1) JP3378255B2 (de)
DE (1) DE19612684A1 (de)
WO (1) WO1997037039A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9807045D0 (en) * 1998-04-01 1998-06-03 Rudi Knut Nucleic acid detection method
CA2421391A1 (en) * 2000-09-08 2002-03-14 Mekbib Astatke Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
CA2537810C (en) 2003-09-04 2012-12-18 Human Genetic Signatures Pty Ltd Nucleic acid detection assay
JP5116481B2 (ja) * 2004-12-03 2013-01-09 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド シトシンの化学修飾により微生物核酸を簡素化するための方法
US8431347B2 (en) * 2005-05-26 2013-04-30 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
EP1943353B1 (de) * 2005-09-14 2011-11-02 Human Genetic Signatures PTY Ltd Gesundheitszustandstest
US20080050738A1 (en) * 2006-05-31 2008-02-28 Human Genetic Signatures Pty Ltd. Detection of target nucleic acid
AU2008229628A1 (en) * 2007-03-16 2008-09-25 Human Genetic Signatures Pty Ltd Assay for gene expression
JP5431351B2 (ja) * 2007-11-27 2014-03-05 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド バイサルファイト修飾された核酸を増幅およびコピーするための酵素
CN101903521A (zh) * 2007-12-20 2010-12-01 人类遗传标记控股有限公司 核酸扩增相关污染物的清除
WO2010026933A1 (ja) * 2008-09-03 2010-03-11 タカラバイオ株式会社 Ｒｎａ検出用組成物
MX350658B (es) 2011-09-07 2017-09-13 Human Genetic Signatures Pty Ltd Ensayo de detección molecular.

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0409078A2 (de) * 1989-07-19 1991-01-23 BASF Aktiengesellschaft Verfahren zur Sequenzierung von Desoxyribonukleinsäuren
EP0655506A1 (de) * 1994-10-17 1995-05-31 President And Fellows Of Harvard College DNS Polymerase mit veränderter Nukleotid-Bindungstelle

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4782137A (en) * 1984-01-24 1988-11-01 Immunex Corporation Synthesis of protein with an identification peptide, and hybrid polypeptide incorporating same
US5489523A (en) * 1990-12-03 1996-02-06 Stratagene Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
AU1035495A (en) * 1993-11-25 1995-06-13 Pacific Enzymes (1993) Limited Improved polymerase
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0409078A2 (de) * 1989-07-19 1991-01-23 BASF Aktiengesellschaft Verfahren zur Sequenzierung von Desoxyribonukleinsäuren
EP0655506A1 (de) * 1994-10-17 1995-05-31 President And Fellows Of Harvard College DNS Polymerase mit veränderter Nukleotid-Bindungstelle

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ELLINGBOE AND GYLLENSTEN: "PCR Technique: DNA Sequencing", 1992, EATON PUBLISHING, NATICK, US, XP002041635, 200190 *
KRETZ K ET AL: "CYCLE SEQUENCING", PCR METHODS & APPLICATIONS, vol. 3, no. 5, April 1994 (1994-04-01), pages S107 - S112, XP000606764 *
MULLIS ET AL.: "The Polymerase Chain Reaction", 1994, BIRKHAUSER, BOSTON, US, XP002041636, 206150 *
SAMBROOK ET AL.: "Molecular cloning", 1987, COLD SPRING HARBOUR PRESS, NY, US, XP002041637, 166120 *

Also Published As

Publication number Publication date
EP0904404A2 (de) 1999-03-31
JP2000503214A (ja) 2000-03-21
DE19612684A1 (de) 1997-10-02
JP3378255B2 (ja) 2003-02-17
WO1997037039A2 (de) 1997-10-09
US6251637B1 (en) 2001-06-26

Similar Documents

Publication Publication Date Title
WO1997037039A3 (de) Verwendung extrem thermophiler dna-polymerasen
WO1995004162A3 (en) Dna sequencing enzymes
CA2310077A1 (en) De novo or "universal" sequencing array
CA2185239A1 (en) Isothermal strand displacement nucleic acid amplification
DE69133204D1 (en) Gereinigtes thermostabiles nukleinsäure-polymeraseenzym aus thermotoga maritima
CA2071196A1 (en) Recombinant expression vectors and purification methods for thermus thermophilus dna polymerase
AU6846798A (en) Method of nucleic acid sequencing
AU6256896A (en) Methods for nucleic acid detection, sequencing, and cloning using exonuclease
EP0370719A3 (de) Erweiterte Nukleotidsequenzen
DE69332666D1 (de) Verfahren zur einfachen nukleotid-primer-verlängerung zum nachweis spezifischer allele und dafür geeigneter kit
EP0246882A3 (en) Art nucleotide segments, vectors cell lines, methods of preparation and use
DK0920501T3 (da) Neuritin, et neurogen
AU1870597A (en) Nucleic acid sequences of genes of the high mobility group proteins and uses thereof
LTIP1519A (en) Solution, set, process for elongation of the oligonucleotidic primer, process of dna amplification
ATE234928T1 (de) Hitzebeständige rekombinante enzyme für die umsetzung von maltose in trehalose
WO1999002544A8 (en) Compounds having energy transfer function and method for dna base sequencing by using the same
EP1083222A3 (de) Endo-Xyloglucan Transferase
ATE65544T1 (de) Verfahren zur herstellung von mesophilen mikroorganismen, die eine bei hoeherer temperatur aktive d-hydantoinase enthalten.
EP0682121A4 (de) Verfahren zur ampflifizierung und zum nachweis bestimmter nukleinsäuresequenzen mittels thermostabiler enzyme.
NO307705B1 (no) Substituerte N-etyl-glycinderivater for fremstilling av PNA og PNA-/DNA-hybrider, samt anvendelse av samme for fremstilling av PNA og PNA-/DNA-hybrider
EP1018559B8 (de) Phosphodiesterasen
AU1981295A (en) Novel protein-kinase, nucleic acid sequences encoding the same and methods related thereto
EP0252497A3 (de) Einspritzung von Schwefeldioxyd zur Mässigung der Temperatur des Ofens für das Claus-Verfahren
GB9519776D0 (en) Materials and methods relating to the transfer of nucleic acid into stem cells
AU697722B2 (en) Novel topoisomerase IV, corresponding nucleotide sequences and uses thereof

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): JP US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE

AK Designated states

Kind code of ref document: A3

Designated state(s): JP US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 1997915455

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1997915455

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 09155512

Country of ref document: US

WWW Wipo information: withdrawn in national office

Ref document number: 1997915455

Country of ref document: EP