WO1997028438B1 - Method and apparatus for multi-spectral analysis in noninvasive nir spectroscopy - Google Patents
Method and apparatus for multi-spectral analysis in noninvasive nir spectroscopyInfo
- Publication number
- WO1997028438B1 WO1997028438B1 PCT/US1997/001370 US9701370W WO9728438B1 WO 1997028438 B1 WO1997028438 B1 WO 1997028438B1 US 9701370 W US9701370 W US 9701370W WO 9728438 B1 WO9728438 B1 WO 9728438B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- radiation
- wavelengths
- analyte
- sample
- filter means
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract 4
- 238000010183 spectrum analysis Methods 0.000 title abstract 2
- 238000004497 NIR spectroscopy Methods 0.000 title 1
- 230000005855 radiation Effects 0.000 claims abstract 34
- 239000012491 analyte Substances 0.000 claims abstract 22
- 230000003287 optical effect Effects 0.000 claims 9
- 238000010521 absorption reaction Methods 0.000 claims 8
- 230000002238 attenuated effect Effects 0.000 claims 4
- 230000003595 spectral effect Effects 0.000 claims 4
- 239000008280 blood Substances 0.000 claims 3
- 210000004369 blood Anatomy 0.000 claims 3
- 238000001514 detection method Methods 0.000 claims 3
- 238000002329 infrared spectrum Methods 0.000 claims 3
- 230000001678 irradiating effect Effects 0.000 claims 3
- 230000007935 neutral effect Effects 0.000 claims 3
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 claims 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 2
- 239000008103 glucose Substances 0.000 claims 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 1
- 238000000862 absorption spectrum Methods 0.000 claims 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims 1
- 239000004202 carbamide Substances 0.000 claims 1
- 230000002596 correlated effect Effects 0.000 claims 1
- 230000000875 corresponding effect Effects 0.000 claims 1
- 235000019441 ethanol Nutrition 0.000 claims 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims 1
- 150000002632 lipids Chemical class 0.000 claims 1
Abstract
A method and apparatus are described for determining the concentration of an analyte present in a sample using multi-spectral analysis in the near infrared range. Incident radiation containing a plurality of distinct, nonoverlapping regions of wavelengths in the range of approximately 1100 to 3500 nm is used to scan a sample. Diffusively reflected radiation emerging from the sample is detected, and a value indicative of the concentration of the analyte is obtained using an application of chemometrics techniques. Information obtained from each nonoverlapping region of wavelengths can be cross-correlated in order to remove background interferences.
Claims
1. An apparatus for determining the concentration of an analyte in a sample, comprising: (a) means for irradiating the sample with incident radiation containing a plurality of distinct, nonoverlapping spectral regions of wavelengths in the near-infrared spectrum;
(b) means for collecting reflected radiation emerging from the sample and directing said reflected radiation into first and second light paths, wherein the first light path comprises radiation from a first spectral region of wavelengths;
(c) first filter means disposed in the first light path, wherein the first filter means is capable of selectively passing radiation having substantially no correlation with the concentration of the analyte;
(d) first detection means for receiving selectively passed radiation emerging from the first filter means and for converting the same into a signal representative of the intensity of said radiation;
(e) adjustable filter means disposed in the second light path, wherein the adjustable filter means attenuates the intensity of radiation in the second light path;
(f) principal analyte filter means capable of receiving attenuated radiation emerging from the adjustable filter means, and selectively passing one or more independent wavelengths therefrom, wherein the one or more independent wavelengths are specifically correlated with the concentration of the analyte;
(g) second filter means capable of receiving the one or more independent wavelengths emerging from the principal analyte filter means and attenuating the intensity of each independent wavelength; and
(h) second detection means for receiving the attenuated independent wavelengths emerging from the second filter means and converting the detected
-35- A ENOED SHEET ARTICLE 19) wavelengths into a signal representative of the intensity of said wavelengths.
2. The apparatus of claim 1, wherein the first filter means comprises a narrow band-pass filter.
3. The apparatus of claim 2, wherein the adjustable filter means comprises a neutral density filter used in concert with correlation filters in a filter system.
4. The apparatus of claim 3, wherein the signal obtained from the first detection means is used to regulate attenuation provided by the adjustable filter means.
5. The apparatus of claim 1, wherein the second filter means comprises a neutral density filter used in concert with correlation filters in a filter system.
6. The apparatus of claim 5, wherein the attenuation provided by the second filter means is established using weighting factors.
7. An apparatus for determining the concentration of an analyte in a sample, comprising:
(a) a source capable of emitting radiation containing a plurality of distinct, nonoverlapping spectral regions in the near-infrared spectrum; (b) means for dividing radiation emitted by the source of part (a) into first and second beam paths; (c) means for irradiating the sample with the radiation in the first beam path, thereby providing reflected radiation; (d) means for collecting the reflected radiation emerging from the sample and directing said reflected radiation into a reflected light path;
-36- (e) a first optical transfer cell disposed in the reflected light path, said first cell comprising first positive correlation filter means having absorption characteristics adapted to accept the reflected radiation and emphasize one or more wavelengths from the reflected radiation, wherein said one or more wavelengths have high correlation with the concentration of the analyte in the sample;
(f) means for receiving the one or more emphasized wavelengths from the first optical transfer cell and for converting the same into signals representative of the intensity of said emphasized wavelengths;
(g) a second optical transfer cell disposed in the second beam path, said second cell comprising neutral density filter means having absorption characteristics sufficient to attenuate the intensity of the radiation from the second beam path equally over a selected range of near-infrared wavelengths;
(h) means for receiving attenuated radiation from the second optical transfer cell and for converting the same into signals representative of the intensity thereof; and
(i) means for calculating the concentration of the analyte in the sample using the signals generated by means (f) and (h) .
8. The apparatus of claim 7, wherein the second optical transfer cell comprises a second positive correlation filter means having absorption characteristics identical to those of the first positive correlation filter means.
9. The apparatus of claim 7, wherein the means for calculating the concentration of the analyte in the sample converts the signals generated by means (f) and
(h) into a digital signal indicative of the ratio of the intensity of the radiation emerging from the source and corresponding radiation emerging from the sample.
10. The apparatus of claim 7, wherein the means for calculating the concentration of the analyte in the sample comprises means for applying a chemometrics algorithm to the signals generated by means (f) and (h) .
11. The apparatus of claim 7, wherein the first positive correlation filter means comprises a plurality of layers, each layer having selected absorption characteristics such that said filter means emphasizes a population of wavelengths having high correlation with the analyte concentration.
12. An apparatus for determining the concentration of an analyte in a sample, comprising: (a) a source capable of emitting radiation containing a plurality of distinct, nonoverlapping spectral regions of wavelength in the near-infrared spectrum;
(b) means for dividing radiation emitted by the source into first and second beam paths;
(c) means for irradiating the sample with the radiation in the first beam path, thereby providing reflected radiation;
(d) means for collecting the reflected radiation emerging from the sample and directing said reflected radiation into a reflected light path;
(e) a first optical transfer cell disposed in the reflected light path, said first cell comprising first positive correlation filter means having absorption characteristics adapted to accept the reflected radiation and emphasize one or more wavelengths from the reflected radiation, wherein said one or more wavelengths have high correlation with the concentration of the analyte in the sample; (f) means for receiving the one or more emphasized wavelengths from the first optical transfer cell and for converting the same into signals representative of the intensity of said emphasized wavelengths;
-38- (g) a second optical transfer cell disposed in the second beam path, said second cell comprising a second positive correlation filter means having absorption characteristics identical to those of the first positive correlation filter means;
(h) means for receiving attenuated radiation from the second optical transfer cell and for converting the same into signals representative of the intensity thereof; and (i) means for calculating the concentration of the analyte in the sample using the signals generated by means (f) and (h) .
13. The apparatus of claim 12, wherein the first positive correlation filter means comprises a plurality of layers, each layer having selected absorption characteristics such that said filter means emphasizes a population of wavelengths having high correlation with the analyte concentration.
14. The apparatus of 13, wherein the absorption characteristics of at least one layer from the first and second positive correlation filter means are established using weighting factors.
15. The apparatus of claims 6 or 14, wherein the weighting factors are derived using chemometrics techniques.
16. The apparatus of claim 15, wherein the weighting factors are derived using rotated principal components analysis of an absorption spectrum of the analyte.
17. The apparatus of claim 1, wherein the wavelengths of the incident radiation are in the range of approximately 1100 to 3500 nm.
-39-
18. The apparatus of claim 7 or 12, wherein the wavelengths of the radiation emitted by the source are in the range of approximately 1100 to 3500 nm.
19. The apparatus of any one of claims 1, 7 or 12, wherein the sample comprises body tissue and the analyte comprises an organic blood analyte.
20. The apparatus of claim 19, wherein the blood analyte is selected from the group consisting of glucose, urea (BUN), lipids, bilirubin and ethyl alcohol.
21. The apparatus of claim 20, wherein the blood analyte is glucose.
-40-
Priority Applications (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL97328060A PL184609B1 (en) | 1996-02-02 | 1997-01-31 | Method of and apparatus for multiple-spectrum non-invasive near ir spectroscopic analysis |
| AU18449/97A AU713502C (en) | 1996-02-02 | 1997-01-31 | Method and apparatus for multi-spectral analysis in noninvasive NIR spectroscopy |
| JP9527784A JPH11506207A (en) | 1996-02-02 | 1997-01-31 | Method and apparatus for multispectral analysis in non-invasive near-infrared spectroscopy |
| DK97904046T DK0877926T3 (en) | 1996-02-02 | 1997-01-31 | Device for multispectral analysis in non-invasive NIR spectroscopy |
| BR9707246-0A BR9707246A (en) | 1996-02-02 | 1997-01-31 | Method and apparatus for multispectral analysis in non-invasive spectroscopy |
| AT97904046T ATE239910T1 (en) | 1996-02-02 | 1997-01-31 | DEVICE FOR MULTISPECTRAL ANALYSIS IN NON-INVASIVE NIR SPECTROSCOPY |
| DE69721732T DE69721732T2 (en) | 1996-02-02 | 1997-01-31 | DEVICE FOR MULTISPEKTRAL ANALYSIS IN NON-INVASIVE NIR SPECTROSCOPY |
| CA002244111A CA2244111C (en) | 1996-02-02 | 1997-01-31 | Method and apparatus for multi-spectral analysis in noninvasive nir spectroscopy |
| HK99104604.2A HK1019635B (en) | 1996-02-02 | 1997-01-31 | Method and apparatus for multi-spectral analysis in noninvasive nir spectroscopy |
| EP97904046A EP0877926B1 (en) | 1996-02-02 | 1997-01-31 | Apparatus for multi-spectral analysis in noninvasive nir spectroscopy |
| NZ331158A NZ331158A (en) | 1996-02-02 | 1997-01-31 | Method and apparatus for multi-spectral analysis in noninvasive nir spectroscopy |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/596,409 | 1996-02-02 | ||
| US08/596,409 US5747806A (en) | 1996-02-02 | 1996-02-02 | Method and apparatus for multi-spectral analysis in noninvasive nir spectroscopy |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1997028438A1 WO1997028438A1 (en) | 1997-08-07 |
| WO1997028438B1 true WO1997028438B1 (en) | 1997-09-12 |
Family
ID=24387178
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1997/001370 WO1997028438A1 (en) | 1996-02-02 | 1997-01-31 | Method and apparatus for multi-spectral analysis in noninvasive nir spectroscopy |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US5747806A (en) |
| EP (1) | EP0877926B1 (en) |
| JP (2) | JPH11506207A (en) |
| KR (1) | KR19990082236A (en) |
| CN (1) | CN1101934C (en) |
| AT (1) | ATE239910T1 (en) |
| BR (1) | BR9707246A (en) |
| CA (1) | CA2244111C (en) |
| CZ (1) | CZ239298A3 (en) |
| DE (1) | DE69721732T2 (en) |
| DK (1) | DK0877926T3 (en) |
| NZ (1) | NZ331158A (en) |
| PL (1) | PL184609B1 (en) |
| TW (1) | TW426802B (en) |
| WO (1) | WO1997028438A1 (en) |
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1997
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- 1997-01-31 CZ CZ982392A patent/CZ239298A3/en unknown
- 1997-01-31 WO PCT/US1997/001370 patent/WO1997028438A1/en not_active Application Discontinuation
- 1997-01-31 JP JP9527784A patent/JPH11506207A/en not_active Withdrawn
- 1997-01-31 DE DE69721732T patent/DE69721732T2/en not_active Expired - Fee Related
- 1997-01-31 EP EP97904046A patent/EP0877926B1/en not_active Expired - Lifetime
- 1997-01-31 KR KR1019980705965A patent/KR19990082236A/en not_active Ceased
- 1997-01-31 AT AT97904046T patent/ATE239910T1/en not_active IP Right Cessation
- 1997-01-31 DK DK97904046T patent/DK0877926T3/en active
- 1997-01-31 CN CN97193402A patent/CN1101934C/en not_active Expired - Fee Related
- 1997-01-31 CA CA002244111A patent/CA2244111C/en not_active Expired - Fee Related
- 1997-01-31 PL PL97328060A patent/PL184609B1/en not_active IP Right Cessation
- 1997-01-31 BR BR9707246-0A patent/BR9707246A/en not_active IP Right Cessation
- 1997-01-31 NZ NZ331158A patent/NZ331158A/en unknown
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1998
- 1998-01-28 US US09/014,933 patent/US5945676A/en not_active Expired - Lifetime
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2002
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US6862091B2 (en) | 2001-04-11 | 2005-03-01 | Inlight Solutions, Inc. | Illumination device and method for spectroscopic analysis |
| US6865408B1 (en) | 2001-04-11 | 2005-03-08 | Inlight Solutions, Inc. | System for non-invasive measurement of glucose in humans |
| US6983176B2 (en) | 2001-04-11 | 2006-01-03 | Rio Grande Medical Technologies, Inc. | Optically similar reference samples and related methods for multivariate calibration models used in optical spectroscopy |
| US7126682B2 (en) | 2001-04-11 | 2006-10-24 | Rio Grande Medical Technologies, Inc. | Encoded variable filter spectrometer |
| US7027848B2 (en) | 2002-04-04 | 2006-04-11 | Inlight Solutions, Inc. | Apparatus and method for non-invasive spectroscopic measurement of analytes in tissue using a matched reference analyte |
| US6958039B2 (en) | 2003-05-02 | 2005-10-25 | Oculir, Inc. | Method and instruments for non-invasive analyte measurement |
| US6968222B2 (en) | 2003-05-02 | 2005-11-22 | Oculir, Inc. | Methods and device for non-invasive analyte measurement |
| US6975892B2 (en) | 2003-10-21 | 2005-12-13 | Oculir, Inc. | Methods for non-invasive analyte measurement from the conjunctiva |
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