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WO1997019350A1 - Procede et solution de rinçage pour instruments d'analyse des fluides biologiques a base de proteines. - Google Patents

Procede et solution de rinçage pour instruments d'analyse des fluides biologiques a base de proteines. Download PDF

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Publication number
WO1997019350A1
WO1997019350A1 PCT/DK1996/000468 DK9600468W WO9719350A1 WO 1997019350 A1 WO1997019350 A1 WO 1997019350A1 DK 9600468 W DK9600468 W DK 9600468W WO 9719350 A1 WO9719350 A1 WO 9719350A1
Authority
WO
WIPO (PCT)
Prior art keywords
rinse solution
rinse
blood
instrument
procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DK1996/000468
Other languages
English (en)
Inventor
Lydia Dahl Clausen
Eiler Larsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Radiometer Medical ApS
Original Assignee
Radiometer Medical ApS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Radiometer Medical ApS filed Critical Radiometer Medical ApS
Publication of WO1997019350A1 publication Critical patent/WO1997019350A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/384—Animal products
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
    • C11D2111/10—Objects to be cleaned
    • C11D2111/14—Hard surfaces
    • C11D2111/20—Industrial or commercial equipment, e.g. reactors, tubes or engines
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1004—Cleaning sample transfer devices

Definitions

  • the present invention relates to a method and a rinse solution used in operating instruments analyzing pro ⁇ tein-containing biological liquids.
  • Instruments analyzing biological liquids are in wide- spread use. Thus, instruments for analyzing blood, se ⁇ rum or plasma in more or less automated form are indis ⁇ pensable in hospitals and laboratories where it is of importance to know the values of various blood para ⁇ meters, e.g. pH, pC0 2 , p0 2 , hemoglobin content, electro- lyte concentration, metabolite concentration, etc. and parameters derived therefrom, e.g. "standard bicarbon ⁇ ate”.
  • Instruments for analysis of blood, serum or plasma typically operate with al ⁇ ternation between an analysis procedure in which a sam- pie is introduced into the instrument for determination of one or more parameters and an rinse procedure in which a rinse solution is passed through the instrument manually or automatically (instrument-driven) .
  • the rinse solution is introduced, either the same way as the sample or, especially for automatic instruments, from a rinse solution reservoir connected to the in ⁇ strument.
  • the normal duration of the rinse procedure is of the same order as the normal duration of each analy ⁇ sis procedure.
  • a calibration procedure is performed during which the sensors of the instrument are calibrated against calibration liquids or gases followed by rinsing with rinse solution. The course of the rinse procedure and calibration procedure is usually determined by the programming of the instru- ment.
  • a source of errors in the determination of blood para ⁇ meters is deposits of a substance on the detection ar ⁇ eas of the sensors, particularly deposits of proteins or protein-containing substances from blood samples analyzed in the instrument.
  • the purpose of the invention is therefore to provide an improved method and rinse solution which can be used in operating various types of blood analysis instruments, including blood analysis instruments containing one or more enzyme sensors.
  • the heparin is preferably present as a heparin salt, most preferably sodium heparin salt.
  • concentration of heparin in the rinse solution is preferably in the range of 200 - 20000 IU/kg, and par ⁇ ticularly in the range of 500 - 10000 IU/kg, the most preferred concentration of heparin in the rinse solu ⁇ tion being 1000 IU/kg rinse solution.
  • a blood dissolving substance desig ⁇ nates a substance capable of dissolving blood or desorbing/removing adsorbed blood proteins.
  • the rinse solution also contains one or more salts of a concentration resulting in a ionic strength of the rinse solution of the same level as the ionic strength of blood.
  • the salt is preferably sodium chloride.
  • the rinse solution contains prefera- bly one or more germicides and one or more surfactants, e.g. KathonTM, GermalTM, TritonTM CF54 and TritonTM X-100.
  • the rinse solution also contains a pH buffer and has a pH value in the range of 6 - 9.
  • the preferred pH buffer is imidazole. Said buffer has i.a. the advantage of not causing interference on the glu ⁇ cose measurement on measuring instruments containing an amperometric glucose sensor.
  • pH buffers are glutaric acid deriva ⁇ tives, e.g. 3, 3-dimethylglutaric acid; barbituric acid derivatives, e.g. ethyl barbituric acid; the so-called Good-buffers having a pK in the range of 6.5 - 9.5, e.g. BES, TES, HEPES, PIPES and MOPS; the tris-buffer (2-amino-2-hydroxymethyl-l, 3-propanediol) and the hy ⁇ drogen phosphate/monohydrogen phosphate buffer system, if said buffers are compatible with the sensors of the blood analysis instrument.
  • glutaric acid deriva ⁇ tives e.g. 3, 3-dimethylglutaric acid
  • barbituric acid derivatives e.g. ethyl barbituric acid
  • the so-called Good-buffers having a pK in the range of 6.5 - 9.5 e.g. BES,
  • a second aspect of the invention relates to a method having the features discussed in the introductory part of claim 1, wherein said method is characterized by the rinse procedure being performed with a rinse solution containing or consisting of a blood dissolving organic substance.
  • the invention also relates to a rinse solution used in the method, said rinse so ⁇ lution being characterized by the rinse solution con ⁇ taining or consisting of a blood dissolving organic substance.
  • the invention further com ⁇ prises the use of a rinse solution containing or con ⁇ sisting of a blood dissolving organic substance in an instrument-driven rinse procedure.
  • the rinse solu ⁇ tion contains preferably salt of a concentration re ⁇ sulting in an ionic strength of the rinse solution of the same level as the ionic strength of blood. Also, it is preferred to add a pH buffer to the rinse solution selected among the pH buffers mentioned above, as it is preferred to add to the rinse solution one or more ger ⁇ macdes or surfactants, particularly the ones mentioned previously.
  • the invention relates to the use of a liquid containing or consisting of a blood dissolving organic substance for rinsing an instrument analyzing protein-containing biological liquids; a method for rinsing an instrument with a liquid containing or con- sisting of a blood dissolving organic substance by a rinse procedure wherein the liquid is introduced into the instrument by the operator the same way as a sample through the normal sample inlet of the instrument; as well as a liquid for use in this rinse procedure, said liquid being characterized by containing or consisting of a blood dissolving substance.
  • Preferred embodiments of said liquid are as described above in connection with the second aspect of the in- vention.
  • the manual rinse procedure can replace or sup- plement the previously discussed instrument-driven rinse procedure.
  • the blood dissolving organic sub- stance forms part of a rinse solution relating to the first aspect of the invention, i.e. a rinse solution containing heparin, or forms part of a rinse solution relating to the second aspect of the invention, i.e. a rinse solution containing or consisting of a blood dis- solving organic substance, but which does not contain an anticoagulant, or forms part of a liquid relating to the third aspect of the invention, in which the liquid is used in a manual rinse procedure, the maximum con ⁇ tent of the blood dissolving organic substance will be determined by the impact of the substance on the sen ⁇ sors of the instrument, whereas the minimum content, which is effective, is determined by means of experi ⁇ ments.
  • the organic blood dissolving substance is selected preferably among diethylene glycol, ethylene glycol, glycerol and compositions thereof;
  • other suitable substances are substances from the group consisting of 2-ethyl hexanol, cyclo- hexane, ethanol amine, dimethyl sulphoxide, formamide, gamma butyrol acetone, 1,3-butane diol, propylene gly ⁇ col, dimethyl formamide, dipropylene glycol, aniline, ethylene glycol monomethyl ether, propylene carbonate, m-cresol, ethanol, methanol, ethylene glycol monoethyl ether, dichlorodiethyl ether, cyclo hexanol, nitroben ⁇ zene, benzaldehyde, 1-butanol, acetophenone, diacetone alcohol, methylene dichloride, ethylene dichloride, ethylene glycol monobutyl ether, isophorone, tetrahy ⁇ drofuran, triethylene glycol, tetraethylene glycol,
  • the most preferred concentration range is 30 ppm - 15000 ppm, more preferred 90 ppm - 1500 ppm, and especially preferred is 150 ppm;

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Dans les instruments d'analyse des fluides biologiques à base de protéines, la procédure de rinçage conduite par l'instrument repose sur l'utilisation d'une solution de rinçage qui contient de l'héparine. De préférence, la solution de rinçage contient aussi une substance organique qui dissout le sang tel que le diéthylèneglycol. Dans une variante, la procédure de rinçage induite par l'instrument repose sur l'utilisation d'une solution de rinçage qui contient une substance organique dissolvant le sang ou qui consiste en une telle substance. Dans une autre variante, une solution contenant ladite substance organique dissolvant le sang ou consistant en une telle substance fait partie intégrante d'une procédure de rinçage manuel pour le rinçage d'instruments d'analyse des fluides biologiques à base de protéines.
PCT/DK1996/000468 1995-11-17 1996-11-14 Procede et solution de rinçage pour instruments d'analyse des fluides biologiques a base de proteines. Ceased WO1997019350A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK1292/95 1995-11-17
DK129295 1995-11-17

Publications (1)

Publication Number Publication Date
WO1997019350A1 true WO1997019350A1 (fr) 1997-05-29

Family

ID=8103198

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK1996/000468 Ceased WO1997019350A1 (fr) 1995-11-17 1996-11-14 Procede et solution de rinçage pour instruments d'analyse des fluides biologiques a base de proteines.

Country Status (1)

Country Link
WO (1) WO1997019350A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9091677B2 (en) 2010-08-09 2015-07-28 Beckman Coulter, Inc. Isotonic buffered composition and method that enables counting of cells
CN105441225A (zh) * 2015-12-18 2016-03-30 杭州毕肯莱博生物科技有限公司 生物仪器管路清洗液及制备方法
CN107034054A (zh) * 2017-04-01 2017-08-11 合肥迪安医学检验所有限公司 一种生化仪比色杯用清洗液

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4127111A (en) * 1976-10-26 1978-11-28 Drolet Roland A Automatic blood sampling system and method
US4867797A (en) * 1979-02-23 1989-09-19 Radiometer A/S Method for cleaning instruments used for analyzing protein-containing biological liquids

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4127111A (en) * 1976-10-26 1978-11-28 Drolet Roland A Automatic blood sampling system and method
US4867797A (en) * 1979-02-23 1989-09-19 Radiometer A/S Method for cleaning instruments used for analyzing protein-containing biological liquids

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9091677B2 (en) 2010-08-09 2015-07-28 Beckman Coulter, Inc. Isotonic buffered composition and method that enables counting of cells
US9567622B2 (en) 2010-08-09 2017-02-14 Beckman Coulter, Inc. Isotonic buffered composition and method that enables counting of cells
CN105441225A (zh) * 2015-12-18 2016-03-30 杭州毕肯莱博生物科技有限公司 生物仪器管路清洗液及制备方法
CN107034054A (zh) * 2017-04-01 2017-08-11 合肥迪安医学检验所有限公司 一种生化仪比色杯用清洗液

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