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WO1997015681B1 - Procedes d'extraction de polyhydroxyalkanoates a partir de vegetaux - Google Patents

Procedes d'extraction de polyhydroxyalkanoates a partir de vegetaux

Info

Publication number
WO1997015681B1
WO1997015681B1 PCT/US1996/016921 US9616921W WO9715681B1 WO 1997015681 B1 WO1997015681 B1 WO 1997015681B1 US 9616921 W US9616921 W US 9616921W WO 9715681 B1 WO9715681 B1 WO 9715681B1
Authority
WO
WIPO (PCT)
Prior art keywords
pha
oil
group
plant
biomass
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1996/016921
Other languages
English (en)
Other versions
WO1997015681A1 (fr
WO1997015681A9 (fr
Filing date
Publication date
Priority claimed from US08/548,840 external-priority patent/US6083729A/en
Application filed filed Critical
Priority to EP96936839A priority Critical patent/EP0859858B1/fr
Priority to CA 2234965 priority patent/CA2234965C/fr
Priority to JP51672197A priority patent/JP2001517070A/ja
Priority to AU74663/96A priority patent/AU722033B2/en
Priority to DE69637253T priority patent/DE69637253T2/de
Publication of WO1997015681A1 publication Critical patent/WO1997015681A1/fr
Publication of WO1997015681B1 publication Critical patent/WO1997015681B1/fr
Publication of WO1997015681A9 publication Critical patent/WO1997015681A9/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Abstract

Cette invention concerne des procédés permettant d'extraire des polyhydroxyalkanoates ('PHA') de végétaux tels que des plantes oléagineuses transgéniques. Ces procédés permettent avantageusement de récupérer à la fois les PHA et l'huile de la biomasse végétale. Dans une réalisation, afin d'isoler les PHA, on prétraite une biomasse dérivée d'une plante oléagineuse, par exemple en procédant à la mouture, l'écrasement ou l'aplatissement par rouleau. Puis on extrait l'huile de la biomasse en utilisant un premier solvant dans lequel l'huile est soluble et dans lequel les PHA ne sont pas fortement solubles de façon à extraire l'huile. On peut ensuite extraire la biomasse en utilisant un second solvant dans lequel le PHA est soluble, de façon à séparer PHA et biomasse. Une autre possibilité consiste à traiter la biomasse contenant les PHA avec un agent chimique ou biochimique, tel qu'un enzyme, de manière à transformer chimiquement le PHA en un dérivé de PHA. On extrait ensuite ce dérivé de PHA du mélange au moyen, par exemple, d'un processus de séparation physique du type distillation, extraction ou chromatographie. La mise en oeuvre de ce procédé permet, avantageusement, d'extraire et de purifier, à grande échelle, les huiles végétales, les PHA et dérivés de PHA, de végétaux contenant de l'huile tels que des végétaux oléagineux transgéniques.
PCT/US1996/016921 1995-10-26 1996-10-23 Procedes d'extraction de polyhydroxyalkanoates a partir de vegetaux Ceased WO1997015681A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP96936839A EP0859858B1 (fr) 1995-10-26 1996-10-23 Procedes d'extraction de polyhydroxyalkanoates a partir de vegetaux
CA 2234965 CA2234965C (fr) 1995-10-26 1996-10-23 Procedes d'extraction de polyhydroxyalkanoates a partir de vegetaux
JP51672197A JP2001517070A (ja) 1995-10-26 1996-10-23 植物からポリヒドロキシアルカノエートを単離する方法
AU74663/96A AU722033B2 (en) 1995-10-26 1996-10-23 Methods for isolating polyhydroxyalkanoates from plants
DE69637253T DE69637253T2 (de) 1995-10-26 1996-10-23 Verfahren zur isolierung von polyhydroxyalkanoaten aus pflanzen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/548,840 US6083729A (en) 1995-10-26 1995-10-26 Methods for isolating polyhydroxyalkanoates from plants
US548,840 1995-10-26

Publications (3)

Publication Number Publication Date
WO1997015681A1 WO1997015681A1 (fr) 1997-05-01
WO1997015681B1 true WO1997015681B1 (fr) 1997-06-19
WO1997015681A9 WO1997015681A9 (fr) 1997-07-17

Family

ID=24190604

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/016921 Ceased WO1997015681A1 (fr) 1995-10-26 1996-10-23 Procedes d'extraction de polyhydroxyalkanoates a partir de vegetaux

Country Status (7)

Country Link
US (2) US6083729A (fr)
EP (1) EP0859858B1 (fr)
JP (4) JP2001517070A (fr)
AT (1) ATE373722T1 (fr)
AU (1) AU722033B2 (fr)
DE (1) DE69637253T2 (fr)
WO (1) WO1997015681A1 (fr)

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