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WO1997013873A1 - Procedure and implements for useful exploitation, caretaking, etc. of a land or water area - Google Patents

Procedure and implements for useful exploitation, caretaking, etc. of a land or water area Download PDF

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Publication number
WO1997013873A1
WO1997013873A1 PCT/FI1995/000567 FI9500567W WO9713873A1 WO 1997013873 A1 WO1997013873 A1 WO 1997013873A1 FI 9500567 W FI9500567 W FI 9500567W WO 9713873 A1 WO9713873 A1 WO 9713873A1
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WO
WIPO (PCT)
Prior art keywords
area
growth
sample
implements
procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FI1995/000567
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French (fr)
Inventor
Matti Reinikainen
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Individual
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Individual
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Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to AU36560/95A priority Critical patent/AU3656095A/en
Priority to EP95934165A priority patent/EP0863996A1/en
Priority to PCT/FI1995/000567 priority patent/WO1997013873A1/en
Publication of WO1997013873A1 publication Critical patent/WO1997013873A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/64—Geomicrobiological testing, e.g. for petroleum

Definitions

  • the present invention relates to a procedure for useful exploitation, tending, determining the con- dition etc. of a land or water area, as defined in claim 1. Moreover, the invention relates to implements for the implementation of the procedure of the inven ⁇ tion, as defined in claim 10.
  • 'Useful exploitation, tending, determining the condition' of an area refers to any producti ⁇ ve or environmental activity concerning the area, such as agriculture, gardening or forestry/afforestation; fertilization, condition monitoring and/or ameliorati ⁇ on of an area; or evaluating, monitoring or improving the overall condition of a land or water area e.g. in conjunction with official environmental surveillance, planning or research or commercial exploitation etc. of an area, yet without being restricted to these examples.
  • productive activities concerning a land area e.g. agriculture, forestry or afforestation
  • a known practice is to examine the con ⁇ dition of the soil to provide a basis for the planning of actions to be taken concerning the area, e.g. to estimate the need for fertilization.
  • the condition of a land area is estimated by carrying out a chemical analysis. Accor ⁇ ding to the current methods, this involves taking one or more samples of the soil and sending them to a re- search laboratory for analysis. Before the chemical analysis is carried out, the samples are dried and powdered.
  • Micro ⁇ bes act as a connection between the animate and ina ⁇ nimate parts of the ecological system. They decompose dead organic matter, thereby binding energy and nutri- ents from plant and animal remains in themselves. Due to decomposition, plants are able to utilize the nut ⁇ rients in the soil. Microbes also affect the composi ⁇ tion of vegetation.
  • microbes are fungi, which make up most of the biomass. For instance there are fungi which live in symbiosis with plant roots, forming a mycorrhiza. For plants, mycorrhiza infection is very important, even indispensable. The mycorrhiza contri ⁇ butes towards a more efficient absorption of nutrients by a plant, maintains its health, protects against stress factors, improves the composition of the soil and reduces erosion and denudation. Other soil micro ⁇ bes and their activities also have a great importance for plants.
  • Unnaturally fast acidification of the soil and intensive use of fertilizers or pesticides, herbi- cides and plant disease fighting agents have a negati ⁇ ve effect on the balance of nature, possibly resulting in unbalanced conditions between different microbes, unnatural populations, or complete disappearance of some species of microbes. For example, it has been es- tablished that certain microbes and root fungi are ve ⁇ ry sensitive to fertilizers or cannot tolerate them at all. An unbalance between microbial populations natu ⁇ rally has a detrimental effect on the condition of the soil and the plants living in it.
  • the object of the present invention is to eliminate the drawbacks described above.
  • a specific object of the present invention is to present a new type of procedure for useful exploi ⁇ tation, tending, determining the condition of a land or water area, which allows a completely different ap ⁇ proach in estimating the condition of a land or water area e.g. for the planning and implementation of pro ⁇ ductive activities in the area, such as farming, fo ⁇ restry or afforestation, and which allows e.g. produc- tive activities concerning the area to be planned and implemented in a manner that takes the natural condi ⁇ tion of the area into account.
  • a further object of the present invention is to present a new type of procedure for useful exploi- tation, tending, determining the condition of a land or water area, in which procedure a sample can be used repeatedly to estimate the condition of the area under investigation without a need to send the sample to a research laboratory.
  • Another object of the present invention is to present means that allow the procedure of the inventi- on to be implemented in simple stages and repeatedly and which can be delivered as an easy-to-use, modera ⁇ te-prized commercial assembly for each application.
  • the procedure of the invention one or mo ⁇ re samples taken from the land or water area concerned are examined to determine the microbial growth in them, the condition of the area is estimated based on said growth, and the growth results obtained are used in the planning of measures or equivalent to be taken concerning the area.
  • the microbial growth can be de ⁇ termined by applying the practices generally known in microbiology, for instance by keeping a sample for a given time in conditions that allow the growth of the microbe under consideration to form a possible micro ⁇ bial population.
  • microbe' refers generally to any microgerms, e.g. bacteria, fungi, yeasts, algae, spores, primordial germ cells, viruses, etc. that may be present in the area under investigation.
  • the microbe to be investigated can be selected from among one or more microbe species rele ⁇ vant for the area under study and the objective of in- vestigation in each case, e.g. a useful or a detrimen ⁇ tal species for plants.
  • 'microbe population' refers to individual cells, colo ⁇ nies, mycelial filaments, including the mycorrhiza in the case of root fungi, etc., whose amounts, quality and/or relationships to each other can be determined. So far, microbial growth estimations have not been used as an aid e.g. in the planning and implemen ⁇ tation of productive activities concerning a land or water area as mentioned above. Estimations based on a chemical analysis as used before do not give any indi- cations about the microbiological condition of a land or water area.
  • plans based on such estimations may instead result in incorrect cul ⁇ tivation/plantation, fertilization etc. with regard to the microbial balance of the area, and this may lead to unfavourable consequences in respect of the growth and health of plants.
  • the procedure of the invention In the procedure of the invention, useful exploitation, tending, determination of the condition etc. of an area are planned and implemented on the ba ⁇ sis of a specified microbial growth, so the procedure makes it possible to implement e.g. farming and fo ⁇ restry in a manner conducive to better conservation and exploitation of nature. Furthermore, due to the estimation of microbial growth as used in the inventi ⁇ on, the procedure allows, among other things, a better estimation of the absorption of nutrients by plants and of the proportion of nutrients in the soil that is useful for plants. Thus, the procedure of the invention can be used as an alternative method or as a complementary method to the chemical methods of estimation so far used in the planning of measures or equivalent concer ⁇ ning a land or water area.
  • the pro ⁇ cedure of the invention is used in the planning of cultivation/growing programmes, soil improvement or fertilization in agriculture or forestry and, if necessary, in the monitoring of the implementation of measures concerning an area, e.g. for monitoring the effects of fertilization or the condition of the soil during the growing season, allowing the implementation to be controlled on the basis of the monitoring re ⁇ sults obtained.
  • seeds/seedlings are germinated/grown in a sample taken from an area under investigation in conjunction with the estimation of microbial growth in order to select on the basis of the germinati ⁇ on/growing results a plant species or variety or a corresponding seedling population suited for the area.
  • the growth results obtained from the microbial growth estimation of the invention are compared with the microbial popu ⁇ lation of the substratum of seedlings to be planted in order to investigate the suitability of the plants for the area, and/or the microbial population of the samp ⁇ le or substantially a population corresponding to the microbial population of the sample is used to infect the roots of the seedlings to be planted prior to planting to obtain a seedling population suited for the area under investigation.
  • the procedure is also excellently suited for the monitoring of the condition of a land or water area e.g. in conjunction with environmental supervisi ⁇ on.
  • the manner of implementation of the procedure of the invention is not limited in any way.
  • the procedure is implemented using implements as pro ⁇ vided by the invention, which comprise
  • the implements of the invention can be fitted to correspond to the area concerned and to the target of investigation.
  • the implements may be available se ⁇ parately or they may be available as a commercial as- sembly.
  • the sample containers (a) are intended for samples taken from the area under investigation for the determination of the occurrence, amount and/or mu ⁇ tual relationships of one or more microbes e.g. by methods known in microbiology.
  • the sample containers may be like those generally used in microbiology.
  • composition of the culture medium can be so selected that it will restrict the growth of unde ⁇ sirable microbes and microbes having no importance with respect to the result.
  • the culture space (b) may be any space ca ⁇ pable of accommodating the sample containers during the growing of the microbes under investigation.
  • a preferred culture space is a culture box known in it- self, which permits regulation of the conditions in it, such as temperature and/or light.
  • the implements of the invention preferably comprise a model file (c) .
  • a model file contains microbe descriptions which may be based on a previous- ly collected verbal description and/or pictorial rep ⁇ resentation of a corresponding microbial growth.
  • the microbial growth as determined from the sample can be compared with the model file with its set of instruc ⁇ tions.
  • the file preferably contains instructions for the interpretation of the results according to the needs of the relevant investigation objectives.
  • the purpose of such a model file is to make it easier to estimate the condition of the area on the basis of the microbial growth produced.
  • the culture mediums and growth conditions in the sample containers preferably correspond to the culture me ⁇ diums of cultures collected in the model file for the microbe under investigation and to the conditions used for those cultures.
  • the model file can be provided with a set of instructions for the planning of actions possibly to be carried out on the area on the basis of the growth results obtained according to the needs of the rele ⁇ vant investigation objectives.
  • the microbial growth samples can be sent to a research laboratory for investigati ⁇ on.
  • the implements of the invention preferably comprise a mea ⁇ suring device for pH measurement known in itself to allow the pH-value of the sample to be determined, in which case the pH-value of the sample can be used to- gether with microbial growth estimation to obtain an assessment of the condition of the area.
  • the implements of the invention are of moderate cost and easy to use. Moreover, the imple- ments allow the procedure of the invention to be car ⁇ ried out repeatedly and reliably.
  • the implements of the invention make it possible e.g. for a producer, such as a farmer, to carry out the procedure him/herself.
  • FIG. 1 presents an embodiment of the imple ⁇ ments of the invention
  • Fig. 2 presents another embodiment of the implements of the invention.
  • the implements of the invention comprise sample containers la, lb, lc, Id.
  • the sample containers are provided with a culture medium, liquid or solid, that allows the growth of the microbe under investigation.
  • a sample container may be designed for one or more microbes. If necessary, the sample containers can be divided into compartments e.g. using partitions lb, lc, Id, and each compartment may contain a different culture medium. In this way, it is possible to obtain an overall analysis or a de ⁇ sired complex of analyses of the same sample.
  • the implements of the invention com ⁇ prise a culture box 2, whose interior temperature is preferably adjustable and which is provided with a light source 5 to produce light in the box when neces ⁇ sary.
  • the implements preferably comprise a model file 3 in which growth descriptions for corres ⁇ ponding microbes have been collected beforehand to al ⁇ low comparisons and which is preferably provided with a set of instructions for estimating the condition of the area and preparing cultivation plans, soil impro- vement programmes and/or fertilization plans on the basis of the growth results obtained.
  • the implements may comprise pH paper for the determination of the pH-value of a samp ⁇ le for the estimation of the condition of the area.
  • the implements may also comprise general instructions for the use of the implements, e.g. instructions relating to growth conditions.
  • a repre ⁇ sentative sample is taken from a field land area to be analysed.
  • the sample is preferably in a natural condi ⁇ tion, but if necessary it can be treated, e.g. elut- riated, without destroying the microbes in it.
  • a sedi ⁇ ment can be prepared using e.g. a ringer solution or a saline solution.
  • the sample is brought onto a culture substrate in a sample container la, lb, lc, Id without touching the sample.
  • the sample container is closed and placed in a culture box 2 where the conditions (light, temperature and/or humidity) preferably cor ⁇ respond to those used in the model file cultures.
  • the sample container 1 Upon the lapse of a given time, the sample container 1 is taken out and the growth formed on the culture substrate is compared with corresponding cul ⁇ ture descriptions in the model file 3 included in the implements, e.g. with respect to the amount, quality and/or mutual relationships of microbial growth.
  • the model file 3 preferably also contains instructions for estimating the condition of the area and for further measures to be taken. Thus, the person carrying out the estimation has the instructions available for in ⁇ terpretation of the results and planning of further measures.
  • Example 1 As an example of the implements of the inven ⁇ tion, implements for examining a field/garden area are presented.
  • the implements comprise:
  • the culture medium for an arbuscular mycorr ⁇ hiza preferably contains the following main nutrients: N, P, K, S, Ca, Mg, Fe; micronutrients: B, Mo, Mn, Co, Zn, Cu, Cl, I; saccharose as a carbohydrate energy source; vitamins: inositol, thiamin, etc.; growth re ⁇ gulators: auxins (IAA, IBA, NAA) , cytokinins (kinetin, BAP, zeatin, 2-4-D) , gibberellins (and antigibberel- lins), coconut milk, casein hydrolysate; active car ⁇ bon; and agar and gelrite as gel forming substances.
  • the culture mediums and growth conditions correspond to those used in the model file.
  • roots are provided in the sample container la.
  • the roots can be obtained from a sample, a parent plant and/or seeds brought into the sample container.
  • the culture box 2 contains a light source 5.
  • seeds/seedlings of different species/varieties as seeds/parent plant to perform a germination/growth test simultaneously with the microbial culture.
  • a ger ⁇ mination test can be used to establish the suitability of different plant species or varieties or equivalent for a given area.
  • a germination test can also be car ⁇ ried out without a microbial estimation.
  • the container la is preferably provided with a cupola-shaped cover, e.g. a hothouse dome 4 .
  • the implements may also include sample con ⁇ tainers la containing a culture medium allowing the growth of the Rhizobium microbe and/or other microbes useful for agriculture.
  • sample con ⁇ tainers lb, lc, Id can be divided with partitions into compartments for different microbes, and each compart ⁇ ment may contain a different culture medium. Overall growth can also be determined. In this case the model file has been complemented by taking all the microbes under investigation into account.
  • the implements of the invention can be implemented by applying general practices asso ⁇ ciated with microbiology; for example, the implements may comprise sample containers for control and/or re ⁇ ference samples.
  • a representative sample (including roots) is taken from the field land area to be investigated. 2. The sample is brought onto a culture sub ⁇ strate in a sample container 1 provided with a hothou ⁇ se dome 4 without touching the sample. A parent plant or plant seeds are brought onto the culture substrate to produce roots and, if desired, to perform a germi- nation test at the same time.
  • the sample container 1 is closed and pro ⁇ vided with information relating to the object of in ⁇ vestigation.
  • the sample container 1 is placed in a cul- ture box 2 in conditions (light, temperature, humidity according to known practice) favourable for an ar- buscular mycorrhiza, corresponding to the conditions used in model file cultures.
  • the sample container 1 is taken out and the growth that has ap ⁇ peared on the culture substrate is compared with cor- responding culture descriptions in the model file 3 comprised in the implements, e.g. as regards the amount, quality and/or mutual relationships of micro ⁇ bial growth.
  • the model file contains instructions for the estimation of the condition of the area and for further measures.
  • the pH of the area under investigation is also determined e.g. from a sediment obtained from a small amount of sample material and the pH-value is used together with the microbial analyses for the estimation of the condition of the area.
  • the infection of roots is an indication of the amount and quality of fungi in the soil under in ⁇ vestigation.
  • determining the growth of a mycorrhiza it is possible to estimate e.g. the nutrient absorpti ⁇ on of plants and/or excessive use of fertilizers (certain fertilizers may destroy the mycorrhiza) .
  • the microbial analysis of the invention can be used to establish whether an in ⁇ tended plantation area has the same/suitable microbe population as the plants have in the nursery.
  • the roots of the plants can be infected with a microbe population corresponding to that in the sample or with the microbe population of the sample prior to planting. If desirable, the infection can al ⁇ so be performed without a microbial analysis.
  • the procedure and implements described in the foregoing can also be applied within the sphere of protection of the invention e.g.
  • the applications may be imple ⁇ mented by making use of the techniques known in micro ⁇ biology.
  • the procedure can also be implemen ⁇ ted by bringing into the sample microbes alien to the area under investigation, in which case the growth or destruction of such microbes in the sample will act as an indicator of the condition of the area.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a procedure for useful exploitation, tending, determining of the condition of a land or water area etc., in which procedure the condition of the area is estimated on the basis of a sample taken from the soil or water and, on the basis of the estimate obtained, measures or equivalent are possibly applied to the area. The procedure is characterized in that microbial growth in the sample taken from the area concerned is determined, the condition of the area is estimated on the basis of said microbial growth and the microbial growth results obtained are used in the planning of measures or equivalent to be applied to the area. The invention also relates to implements designed for the implementation of the procedure of the invention.

Description

PROCEDURE AND IMPLEMENTS FOR USEFUL EXPLOITATION, CARETAKING, ETC. OF A LAND OR WATER AREA
The present invention relates to a procedure for useful exploitation, tending, determining the con- dition etc. of a land or water area, as defined in claim 1. Moreover, the invention relates to implements for the implementation of the procedure of the inven¬ tion, as defined in claim 10.
'Useful exploitation, tending, determining the condition' of an area here refers to any producti¬ ve or environmental activity concerning the area, such as agriculture, gardening or forestry/afforestation; fertilization, condition monitoring and/or ameliorati¬ on of an area; or evaluating, monitoring or improving the overall condition of a land or water area e.g. in conjunction with official environmental surveillance, planning or research or commercial exploitation etc. of an area, yet without being restricted to these examples. In conjunction with productive activities concerning a land area, e.g. agriculture, forestry or afforestation, a known practice is to examine the con¬ dition of the soil to provide a basis for the planning of actions to be taken concerning the area, e.g. to estimate the need for fertilization.
In prior art, the condition of a land area is estimated by carrying out a chemical analysis. Accor¬ ding to the current methods, this involves taking one or more samples of the soil and sending them to a re- search laboratory for analysis. Before the chemical analysis is carried out, the samples are dried and powdered.
The current methods of analysis have the drawback that, even though they are designed for the estimation of the condition of the soil in respect of nutrients, chemical methods are still not adequate for specifically determining the portion of the nutrient content of the soil that is useful for plants, because such methods cannot be used to simulate the absorption of nutrients by plant roots, which varies considerably depending on external conditions. Therefore, with che- mical methods it is only possible to determine a cer¬ tain soluble portion of the nutrients. In practice, the properties affecting nutrient supply, such as aci¬ dity, water content, compactness etc. of the soil, show such great variations that it is sometimes diffi- cult to establish a clear dependence between the con¬ tent of soluble nutrients in the soil and the nutrient supply of plants. Furthermore, the drying and crushing of the samples changes the properties of natural soil, including the solubility of nutrients, and these chan- ges are different in different types of soil. The abo¬ ve-mentioned factors to some extent reduce the practi¬ cal applicability of the results.
Environmental conditions, such as the degree of pollutedness, e.g. the occurrence of heavy metals, have also so far been established and monitored on the basis of chemical analyses.
Current methods of investigation are therefo¬ re based on only determining the amounts of compounds and elements in samples. However, the microfauna has a substantial effect on the soil and as well as the growth of plants and their nutrient absorption. Micro¬ bes act as a connection between the animate and ina¬ nimate parts of the ecological system. They decompose dead organic matter, thereby binding energy and nutri- ents from plant and animal remains in themselves. Due to decomposition, plants are able to utilize the nut¬ rients in the soil. Microbes also affect the composi¬ tion of vegetation.
An example of microbes are fungi, which make up most of the biomass. For instance there are fungi which live in symbiosis with plant roots, forming a mycorrhiza. For plants, mycorrhiza infection is very important, even indispensable. The mycorrhiza contri¬ butes towards a more efficient absorption of nutrients by a plant, maintains its health, protects against stress factors, improves the composition of the soil and reduces erosion and denudation. Other soil micro¬ bes and their activities also have a great importance for plants.
Unnaturally fast acidification of the soil and intensive use of fertilizers or pesticides, herbi- cides and plant disease fighting agents have a negati¬ ve effect on the balance of nature, possibly resulting in unbalanced conditions between different microbes, unnatural populations, or complete disappearance of some species of microbes. For example, it has been es- tablished that certain microbes and root fungi are ve¬ ry sensitive to fertilizers or cannot tolerate them at all. An unbalance between microbial populations natu¬ rally has a detrimental effect on the condition of the soil and the plants living in it. The object of the present invention is to eliminate the drawbacks described above.
A specific object of the present invention is to present a new type of procedure for useful exploi¬ tation, tending, determining the condition of a land or water area, which allows a completely different ap¬ proach in estimating the condition of a land or water area e.g. for the planning and implementation of pro¬ ductive activities in the area, such as farming, fo¬ restry or afforestation, and which allows e.g. produc- tive activities concerning the area to be planned and implemented in a manner that takes the natural condi¬ tion of the area into account.
A further object of the present invention is to present a new type of procedure for useful exploi- tation, tending, determining the condition of a land or water area, in which procedure a sample can be used repeatedly to estimate the condition of the area under investigation without a need to send the sample to a research laboratory.
Another object of the present invention is to present means that allow the procedure of the inventi- on to be implemented in simple stages and repeatedly and which can be delivered as an easy-to-use, modera¬ te-prized commercial assembly for each application.
As for the features characteristic of the in¬ vention, reference is made to the claims. In the procedure of the invention, one or mo¬ re samples taken from the land or water area concerned are examined to determine the microbial growth in them, the condition of the area is estimated based on said growth, and the growth results obtained are used in the planning of measures or equivalent to be taken concerning the area. The microbial growth can be de¬ termined by applying the practices generally known in microbiology, for instance by keeping a sample for a given time in conditions that allow the growth of the microbe under consideration to form a possible micro¬ bial population. In the estimation of the condition of the area on the basis of the growth results and in the planning of further measures or equivalent concerning the area, it is possible to use e.g. existing informa- tion about the microbe/microbes under investigation or an instruction file provided for the procedure of the invention.
The term 'microbe' as used in this context refers generally to any microgerms, e.g. bacteria, fungi, yeasts, algae, spores, primordial germ cells, viruses, etc. that may be present in the area under investigation. The microbe to be investigated can be selected from among one or more microbe species rele¬ vant for the area under study and the objective of in- vestigation in each case, e.g. a useful or a detrimen¬ tal species for plants.
As is known in the field of microbiology, 'microbe population' refers to individual cells, colo¬ nies, mycelial filaments, including the mycorrhiza in the case of root fungi, etc., whose amounts, quality and/or relationships to each other can be determined. So far, microbial growth estimations have not been used as an aid e.g. in the planning and implemen¬ tation of productive activities concerning a land or water area as mentioned above. Estimations based on a chemical analysis as used before do not give any indi- cations about the microbiological condition of a land or water area. For example, in conjunction with agri¬ culture and forestry/afforestation, plans based on such estimations may instead result in incorrect cul¬ tivation/plantation, fertilization etc. with regard to the microbial balance of the area, and this may lead to unfavourable consequences in respect of the growth and health of plants.
In the procedure of the invention, useful exploitation, tending, determination of the condition etc. of an area are planned and implemented on the ba¬ sis of a specified microbial growth, so the procedure makes it possible to implement e.g. farming and fo¬ restry in a manner conducive to better conservation and exploitation of nature. Furthermore, due to the estimation of microbial growth as used in the inventi¬ on, the procedure allows, among other things, a better estimation of the absorption of nutrients by plants and of the proportion of nutrients in the soil that is useful for plants. Thus, the procedure of the invention can be used as an alternative method or as a complementary method to the chemical methods of estimation so far used in the planning of measures or equivalent concer¬ ning a land or water area. According to a preferred embodiment, the pro¬ cedure of the invention is used in the planning of cultivation/growing programmes, soil improvement or fertilization in agriculture or forestry and, if necessary, in the monitoring of the implementation of measures concerning an area, e.g. for monitoring the effects of fertilization or the condition of the soil during the growing season, allowing the implementation to be controlled on the basis of the monitoring re¬ sults obtained.
In an additional embodiment of the procedure of the invention, seeds/seedlings are germinated/grown in a sample taken from an area under investigation in conjunction with the estimation of microbial growth in order to select on the basis of the germinati¬ on/growing results a plant species or variety or a corresponding seedling population suited for the area. Further, in another additional embodiment, the growth results obtained from the microbial growth estimation of the invention are compared with the microbial popu¬ lation of the substratum of seedlings to be planted in order to investigate the suitability of the plants for the area, and/or the microbial population of the samp¬ le or substantially a population corresponding to the microbial population of the sample is used to infect the roots of the seedlings to be planted prior to planting to obtain a seedling population suited for the area under investigation.
The procedure is also excellently suited for the monitoring of the condition of a land or water area e.g. in conjunction with environmental supervisi¬ on. The manner of implementation of the procedure of the invention is not limited in any way. Preferably the procedure is implemented using implements as pro¬ vided by the invention, which comprise
(a) at least one sample container for each microbe or group of microbes under investigation, said sample container containing a culture medium suitable for the microbe or group of microbes; (b) a culture space for the sample containers to create conditions favourable for the growth of each microbe or group of microbes under investigation; and optionally (c) a model file in which growth descriptions for each microbe or group of microbes under investiga¬ tion have been collected beforehand to allow compari¬ sons between growth samples and which is provided with a set of instructions for estimating the condition of the area on the basis of the growth results obtained.
The implements of the invention can be fitted to correspond to the area concerned and to the target of investigation. The implements may be available se¬ parately or they may be available as a commercial as- sembly.
The sample containers (a) are intended for samples taken from the area under investigation for the determination of the occurrence, amount and/or mu¬ tual relationships of one or more microbes e.g. by methods known in microbiology. The sample containers may be like those generally used in microbiology.
The composition of the culture medium can be so selected that it will restrict the growth of unde¬ sirable microbes and microbes having no importance with respect to the result.
The culture space (b) may be any space ca¬ pable of accommodating the sample containers during the growing of the microbes under investigation. A preferred culture space is a culture box known in it- self, which permits regulation of the conditions in it, such as temperature and/or light.
In addition, the implements of the invention preferably comprise a model file (c) . It contains microbe descriptions which may be based on a previous- ly collected verbal description and/or pictorial rep¬ resentation of a corresponding microbial growth. To estimate the biological condition of the area, the microbial growth as determined from the sample can be compared with the model file with its set of instruc¬ tions. The file preferably contains instructions for the interpretation of the results according to the needs of the relevant investigation objectives. The purpose of such a model file is to make it easier to estimate the condition of the area on the basis of the microbial growth produced.
When the implements include a model file com- posed beforehand for the application in question, the culture mediums and growth conditions in the sample containers preferably correspond to the culture me¬ diums of cultures collected in the model file for the microbe under investigation and to the conditions used for those cultures.
According to a specially preferred embodi¬ ment, the model file can be provided with a set of instructions for the planning of actions possibly to be carried out on the area on the basis of the growth results obtained according to the needs of the rele¬ vant investigation objectives.
Alternatively, the microbial growth samples can be sent to a research laboratory for investigati¬ on. According to a preferred embodiment, the implements of the invention preferably comprise a mea¬ suring device for pH measurement known in itself to allow the pH-value of the sample to be determined, in which case the pH-value of the sample can be used to- gether with microbial growth estimation to obtain an assessment of the condition of the area.
As compared with prior-art equipment for che¬ mical analyses, the implements of the invention are of moderate cost and easy to use. Moreover, the imple- ments allow the procedure of the invention to be car¬ ried out repeatedly and reliably. The implements of the invention make it possible e.g. for a producer, such as a farmer, to carry out the procedure him/herself.
In the following, the invention is described by referring to the drawings, in which Fig. 1 presents an embodiment of the imple¬ ments of the invention, and
Fig. 2 presents another embodiment of the implements of the invention.
As shown in Fig. 1 and 2, the implements of the invention comprise sample containers la, lb, lc, Id. The sample containers are provided with a culture medium, liquid or solid, that allows the growth of the microbe under investigation. A sample container may be designed for one or more microbes. If necessary, the sample containers can be divided into compartments e.g. using partitions lb, lc, Id, and each compartment may contain a different culture medium. In this way, it is possible to obtain an overall analysis or a de¬ sired complex of analyses of the same sample. Further, the implements of the invention com¬ prise a culture box 2, whose interior temperature is preferably adjustable and which is provided with a light source 5 to produce light in the box when neces¬ sary. Moreover, the implements preferably comprise a model file 3 in which growth descriptions for corres¬ ponding microbes have been collected beforehand to al¬ low comparisons and which is preferably provided with a set of instructions for estimating the condition of the area and preparing cultivation plans, soil impro- vement programmes and/or fertilization plans on the basis of the growth results obtained.
Furthermore, the implements may comprise pH paper for the determination of the pH-value of a samp¬ le for the estimation of the condition of the area. The implements may also comprise general instructions for the use of the implements, e.g. instructions relating to growth conditions. In the procedure of the invention, a repre¬ sentative sample is taken from a field land area to be analysed. The sample is preferably in a natural condi¬ tion, but if necessary it can be treated, e.g. elut- riated, without destroying the microbes in it. A sedi¬ ment can be prepared using e.g. a ringer solution or a saline solution. The sample is brought onto a culture substrate in a sample container la, lb, lc, Id without touching the sample. The sample container is closed and placed in a culture box 2 where the conditions (light, temperature and/or humidity) preferably cor¬ respond to those used in the model file cultures.
Upon the lapse of a given time, the sample container 1 is taken out and the growth formed on the culture substrate is compared with corresponding cul¬ ture descriptions in the model file 3 included in the implements, e.g. with respect to the amount, quality and/or mutual relationships of microbial growth. The model file 3 preferably also contains instructions for estimating the condition of the area and for further measures to be taken. Thus, the person carrying out the estimation has the instructions available for in¬ terpretation of the results and planning of further measures. In the following examples, an embodiment of the implements and of the procedure of the invention is described by referring to Fig. 1 and 2.
Example 1: As an example of the implements of the inven¬ tion, implements for examining a field/garden area are presented. The implements comprise:
(a) sample containers la containing a culture medium that allows the growth of an arbuscular mycorr- hiza;
(b) a culture box in which it is possible to create conditions favourable for the growth of an ar- buscular mycorrhiza;
(c) a model file in which growth descriptions for a corresponding mycorrhiza have been collected be¬ forehand and which contains a set of instructions for estimating the condition of the area and preparing cultivation plans, soil improvement programmes and/or fertilization plans on the basis of the growth results obtained.
(d) pH paper for determining the pH-value of the sample.
The culture medium for an arbuscular mycorr¬ hiza preferably contains the following main nutrients: N, P, K, S, Ca, Mg, Fe; micronutrients: B, Mo, Mn, Co, Zn, Cu, Cl, I; saccharose as a carbohydrate energy source; vitamins: inositol, thiamin, etc.; growth re¬ gulators: auxins (IAA, IBA, NAA) , cytokinins (kinetin, BAP, zeatin, 2-4-D) , gibberellins (and antigibberel- lins), coconut milk, casein hydrolysate; active car¬ bon; and agar and gelrite as gel forming substances. The culture mediums and growth conditions correspond to those used in the model file.
To allow the formation of a mycorrhiza, roots are provided in the sample container la. The roots can be obtained from a sample, a parent plant and/or seeds brought into the sample container. To permit germina¬ tion/growth of the seeds/plants, the culture box 2 contains a light source 5. If desired, it is possible to use seeds/seedlings of different species/varieties as seeds/parent plant to perform a germination/growth test simultaneously with the microbial culture. A ger¬ mination test can be used to establish the suitability of different plant species or varieties or equivalent for a given area. A germination test can also be car¬ ried out without a microbial estimation. When seeds/plants are germinated/grown in a sample container la, the container la is preferably provided with a cupola-shaped cover, e.g. a hothouse dome 4 .
The implements may also include sample con¬ tainers la containing a culture medium allowing the growth of the Rhizobium microbe and/or other microbes useful for agriculture. Alternatively, the sample con¬ tainers lb, lc, Id can be divided with partitions into compartments for different microbes, and each compart¬ ment may contain a different culture medium. Overall growth can also be determined. In this case the model file has been complemented by taking all the microbes under investigation into account.
In general, the implements of the invention can be implemented by applying general practices asso¬ ciated with microbiology; for example, the implements may comprise sample containers for control and/or re¬ ference samples.
Example 2:
The procedure of the invention for investiga- ting a field land area for cultivation and/or fertili¬ zation plans is carried out using implements as described in Example 1.
1. A representative sample (including roots) is taken from the field land area to be investigated. 2. The sample is brought onto a culture sub¬ strate in a sample container 1 provided with a hothou¬ se dome 4 without touching the sample. A parent plant or plant seeds are brought onto the culture substrate to produce roots and, if desired, to perform a germi- nation test at the same time.
3. The sample container 1 is closed and pro¬ vided with information relating to the object of in¬ vestigation.
4. The sample container 1 is placed in a cul- ture box 2 in conditions (light, temperature, humidity according to known practice) favourable for an ar- buscular mycorrhiza, corresponding to the conditions used in model file cultures.
5. Upon the lapse of a given time, the sample container 1 is taken out and the growth that has ap¬ peared on the culture substrate is compared with cor- responding culture descriptions in the model file 3 comprised in the implements, e.g. as regards the amount, quality and/or mutual relationships of micro¬ bial growth. The model file contains instructions for the estimation of the condition of the area and for further measures.
In addition to microbial growth, the pH of the area under investigation is also determined e.g. from a sediment obtained from a small amount of sample material and the pH-value is used together with the microbial analyses for the estimation of the condition of the area.
The infection of roots is an indication of the amount and quality of fungi in the soil under in¬ vestigation. By determining the growth of a mycorrhiza it is possible to estimate e.g. the nutrient absorpti¬ on of plants and/or excessive use of fertilizers (certain fertilizers may destroy the mycorrhiza) .
Further, according to an embodiment, since e.g. the plants of forest trees are grown e.g. in nur- series on different substrates as compared with the area under investigation, the microbial analysis of the invention can be used to establish whether an in¬ tended plantation area has the same/suitable microbe population as the plants have in the nursery. Thus, if necessary, the roots of the plants can be infected with a microbe population corresponding to that in the sample or with the microbe population of the sample prior to planting. If desirable, the infection can al¬ so be performed without a microbial analysis. The procedure and implements described in the foregoing can also be applied within the sphere of protection of the invention e.g. for establishing the health of the soil in an area under investigation or of a water system, for the investigation of wood land and bioenergy or for the measurement of pollution in the soil and in water systems. As for microbial analy- ses and instructions, the applications may be imple¬ mented by making use of the techniques known in micro¬ biology.
Further, the procedure can also be implemen¬ ted by bringing into the sample microbes alien to the area under investigation, in which case the growth or destruction of such microbes in the sample will act as an indicator of the condition of the area.
The examples are only intended to provide an illustration of the invention, without limiting it in any way.

Claims

1. Procedure for useful exploitation, ten¬ ding, or determining of the condition of a land or wa¬ ter area, in which procedure the condition of the area is estimated on the basis of a sample taken from the soil or water and, on the basis of the estimate obtained, measures or equivalent are possibly applied to the area, c h a r a c t e r i z e d in that microbial growth in the sample taken from the area concerned is determined, the condition of the area is estimated on the basis of said microbial growth and the microbial growth results obtained are used in the planning of measures or equivalent to be applied to the area.
2. Procedure as defined in claim 1, c h a - r a c t e r i z e d in that the microbial growth as de¬ termined from the sample is compared with a model file composed beforehand, presenting growth descriptions for the corresponding microbe, to estimate the biolo¬ gical condition of the area, and the measures possibly to be applied to the area are planned on the basis of the results obtained.
3. Procedure as defined in claim 1 or 2, c h a r a c t e r i z e d in that the sample is kept for a given time in conditions allowing the growth of the microbe under investigation to produce a possible po¬ pulation of the microbe.
4. Procedure as defined in claim 3, c h a ¬ r a c t e r i z e d in that the sample is kept in growth conditions corresponding to the growth conditions of cultures of the corresponding microbe collected in the model file.
5. Procedure as defined in any one of claims 1 - 4, c h a r a c t e r i z e d in that the pH of the sample is determined for the estimation of the condi- tion of the area.
6. Procedure as defined in any one of claims 1 - 5, c h a r a c t e r i z e d in that the microbial growth analysis is used in the planning and monitoring of implementation of measures associated with agricul¬ ture, gardening, forestry or in the monitoring of the condition of a land or water area or in the planning of its improvement.
7. Procedure as defined in any one of claims 1 - 6, c h a r a c t e r i z e d in that at least one sample is taken from a field land area and the growth of root fungi is determined e.g. as the growth of a mycorrhiza, and possibly the growth of the Rhizobium microbe is determined; the population produced is com¬ pared with a model file composed of descriptions of corresponding microbe populations and the results obtained are used as a basis for cultivation plans, soil improvement programmes, fertilization plans etc.
8. Procedure as defined in any one of claims 1 - 7, c h a r a c t e r i z e d in that in conjunction with the determination of microbial growth in the sample, seeds/plants are germinated/grown in the samp- le to allow the selection of a plant species or varie¬ ty or a corresponding seedling population suited for the area on the basis of the germination/growth re¬ sults obtained.
9. Procedure as defined in any one of claims 1 - 8, c h a r a c t e r i z e d in that
(a) the microbial growth as determined from a sample taken from the area under investigation is com¬ pared with the microbial population in the substratum for seedlings to be planted in order to establish the suitability of the plants to the area; and/or
(b) the microbial population of the sample or substantially a microbial population corresponding to the microbial population of the sample is used to in¬ fect the roots of the seedlings to be planted prior to planting to obtain a seedling population suited for the area under investigation.
10. Implements for use in the procedure of the invention, in which the condition of a land or wa¬ ter area is estimated on the basis of a sample taken from the soil or water and, based on the estimate obtained, measures or equivalent are applied to the area, c h a r a c t e r i z e d in that the implements comprise
(a) at least one sample container for each microbe or group of microbes under investigation, said sample container containing a culture medium suitable for the microbe or group of microbes;
(b) a culture space for the sample containers to create conditions favourable for the growth of each microbe or group of microbes under investigation; and optionally (c) a model file in which growth descriptions for each microbe or group of microbes under investiga¬ tion have been collected beforehand to allow compari¬ sons between growth samples and which is provided with a set of instructions for estimating the condition of the area on the basis of the growth results obtained.
11. Implements as defined in claim 10, c h a r a c t e r i z e d in that the model file is pro¬ vided with a set of instructions for the planning of actions possibly to be carried out on the area on the basis of the growth results obtained.
12. Implements as defined in claim 10 or 11, c h a r a c t e r i z e d in that the culture mediums correspond to the culture mediums used in the cultures of the microbe under investigation that are included in the model file and the conditions in the culture space can be made to correspond to the conditions of the cultures of the microbe under investigation that are included in the model file.
13. Implements as defined in claim 10 or 12, c h a r a c t e r i z e d in that the implements comprise a pH measuring device for the determination of the pH- value of the sample.
14. Implements as defined in claim 10 or 13, c h a r a c t e r i z e d in that the implements are con¬ sistent with the object under investigation and the objective of investigation in each case.
15. Implements as defined in claim 10 or 14, c h a r a c t e r i z e d in that the implements are in¬ tended for use in the planning of measures related to agriculture, gardening or forestry/afforestation or for the establishment, monitoring or improvement of the condition of a land or water area.
16. Implements as defined in any one of claims 10 - 15, c h a r a c t e r i z e d in that the implements are fitted for the planning of the utilisa¬ tion of a field land area intended for agricultural purposes and the implements comprise
(a) one or more sample containers containing a culture medium that allows the growth of an arbuscu- le, e.g. a mycorrhiza, and/or a culture medium that allows the growth of the Rhizobium microbe. (b) a culture box in which the conditions can be made favourable for the microbes to be grown;
(c) a model file which contains a collection of growth descriptions for corresponding microbes and a set of instructions for estimating the condition of the area under investigation and for preparing culti¬ vation plans, soil improvement programmes etc. on the basis of the growth results obtained.
PCT/FI1995/000567 1995-10-12 1995-10-12 Procedure and implements for useful exploitation, caretaking, etc. of a land or water area Ceased WO1997013873A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
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EP95934165A EP0863996A1 (en) 1995-10-12 1995-10-12 Procedure and implements for useful exploitation, caretaking, etc. of a land or water area
PCT/FI1995/000567 WO1997013873A1 (en) 1995-10-12 1995-10-12 Procedure and implements for useful exploitation, caretaking, etc. of a land or water area

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001080648A1 (en) * 2000-04-25 2001-11-01 Neoforest Oy Method for sowing seeds
NL1022152C2 (en) * 2002-12-12 2004-06-18 Tno Process control based on analysis of microbial populations.

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3236600A (en) * 1963-05-02 1966-02-22 Shrimpton Wallace Soil test method
DE4027284A1 (en) * 1990-08-29 1992-03-05 Enders Josef Microorganism treatment of soil extracts or liq. samples - by enriching sample with anions or cations in test series, adding microorganism-contg. soln. and measuring deviation from neutrality

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3236600A (en) * 1963-05-02 1966-02-22 Shrimpton Wallace Soil test method
DE4027284A1 (en) * 1990-08-29 1992-03-05 Enders Josef Microorganism treatment of soil extracts or liq. samples - by enriching sample with anions or cations in test series, adding microorganism-contg. soln. and measuring deviation from neutrality

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001080648A1 (en) * 2000-04-25 2001-11-01 Neoforest Oy Method for sowing seeds
NL1022152C2 (en) * 2002-12-12 2004-06-18 Tno Process control based on analysis of microbial populations.
WO2004053147A1 (en) * 2002-12-12 2004-06-24 Nederlandse Organisatie Voor Toegepast- Natuurwetenschappelijk Onderzoek Tno Process control based on analysis of microbial populations

Also Published As

Publication number Publication date
AU3656095A (en) 1997-04-30
EP0863996A1 (en) 1998-09-16

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