[go: up one dir, main page]

WO1996036881A3 - Screening for inhibitors of tcr-mhc interactions - Google Patents

Screening for inhibitors of tcr-mhc interactions Download PDF

Info

Publication number
WO1996036881A3
WO1996036881A3 PCT/GB1996/001165 GB9601165W WO9636881A3 WO 1996036881 A3 WO1996036881 A3 WO 1996036881A3 GB 9601165 W GB9601165 W GB 9601165W WO 9636881 A3 WO9636881 A3 WO 9636881A3
Authority
WO
WIPO (PCT)
Prior art keywords
tcr
mhc
peptide ligand
responder cells
signal produced
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB1996/001165
Other languages
French (fr)
Other versions
WO1996036881A2 (en
Inventor
Dan Hung Barouch
Stephen John Rupert Vessey
Bent Karsten Jakobsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cancer Research Campaign Technology Ltd
Original Assignee
Cancer Research Campaign Technology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cancer Research Campaign Technology Ltd filed Critical Cancer Research Campaign Technology Ltd
Priority to AU57690/96A priority Critical patent/AU5769096A/en
Publication of WO1996036881A2 publication Critical patent/WO1996036881A2/en
Publication of WO1996036881A3 publication Critical patent/WO1996036881A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56977—HLA or MHC typing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/7051—T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70539—MHC-molecules, e.g. HLA-molecules

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

This invention relates to the interactions between T-cell receptors and their specific MHC-peptide ligand. A screening method is provided to identify substances which inhibit the specific interaction between a T-cell receptor (TCR) and an MHC-peptide ligand involving incubating responder cells expressing a TCR with (i) an MHC-peptide ligand which stimulates said responder cells; and (ii) a candidate inhibitory substance; and monitoring a signal produced by said responder cells when the TCR engages the MHC-peptide ligand; and comparing said signal produced by said responder cells on the engagement of TCR with the MHC-peptide ligand with a control signal produced by said responder cells when incubated with (i) but not (ii); wherein a reduction in signal associated with the presence of (ii) in the incubation represents the efficacy of the inhibitor.
PCT/GB1996/001165 1995-05-16 1996-05-16 Screening for inhibitors of tcr-mhc interactions Ceased WO1996036881A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU57690/96A AU5769096A (en) 1995-05-16 1996-05-16 Screening for inhibitors of tcr-mhc interactions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9509844.8A GB9509844D0 (en) 1995-05-16 1995-05-16 Screening for inhibitors of TCR-MHC interactions
GB9509844.8 1995-05-16

Publications (2)

Publication Number Publication Date
WO1996036881A2 WO1996036881A2 (en) 1996-11-21
WO1996036881A3 true WO1996036881A3 (en) 1997-01-09

Family

ID=10774510

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1996/001165 Ceased WO1996036881A2 (en) 1995-05-16 1996-05-16 Screening for inhibitors of tcr-mhc interactions

Country Status (3)

Country Link
AU (1) AU5769096A (en)
GB (1) GB9509844D0 (en)
WO (1) WO1996036881A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996031625A1 (en) 1995-04-07 1996-10-10 Cytogen Corporation Polypeptides having a functional domain of interest and methods of identifying and using same
US6309820B1 (en) 1995-04-07 2001-10-30 University Of North Carolina At Chapel Hill Polypeptides having a functional domain of interest and methods of identifying and using same
AU3036597A (en) * 1996-05-21 1997-12-09 Institut National De La Sante Et De La Recherche Medicale (Inserm) Method for using peptide complexes/major histocompatibility complex to obtain or purify antigen-specific t cells and to stimulate t cells
AU2481399A (en) * 1998-01-29 1999-08-16 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Variant peptide ligands that selectively induce apoptosis
US20040253632A1 (en) * 2000-05-25 2004-12-16 Sunol Molecular Corporation Modulation of T -cell receptor interactions
US20210371849A1 (en) * 2018-07-31 2021-12-02 Janux Therapeutics, Inc. Inhibitory t cell receptor peptides and discovery methods

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992016234A1 (en) * 1991-03-22 1992-10-01 The Trustees Of The University Of Pennsylvania Method of modulating mammalian t-cell response
WO1995007707A1 (en) * 1993-09-14 1995-03-23 Cytel Corporation Alteration of immune response using pan dr-binding peptides

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992016234A1 (en) * 1991-03-22 1992-10-01 The Trustees Of The University Of Pennsylvania Method of modulating mammalian t-cell response
WO1995007707A1 (en) * 1993-09-14 1995-03-23 Cytel Corporation Alteration of immune response using pan dr-binding peptides

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
J. MADRENAS ET AL.: "Zeta phosphorylation without ZAP-70 activation induced by TcR antagonists or partial agonists.", SCIENCE, vol. 267, no. 5197, 27 January 1995 (1995-01-27), WASHINGTON, DC, USA, pages 515 - 518, XP002015729 *
S. JAMESON ET AL.: "Clone-specific T cell receptor antagonists of major histocompatibility complex class I-restricted cytotoxic T cells.", THE JOURNAL OF EXPERIMENTAL MEDICINE, vol. 177, no. 6, 1 June 1993 (1993-06-01), NEW YORK, NY, USA, pages 1541 - 1550, XP000604978 *
S. WEBER ET AL.: "Specific low-affinity recognition of major histocompatibility complex plus peptide by soluble T-cell receptor.", NATURE, vol. 356, 30 April 1992 (1992-04-30), LONDON, GB, pages 793 - 796, XP002008364 *
W. WILLIAMS ET AL.: "Modulation of T cell responses with MHC-derived peptides.", IMMUNOLOGICAL RESEARCH, vol. 11, no. 1, March 1992 (1992-03-01), BASEL, SWITZERLAND, pages 11 - 23, XP002015728 *

Also Published As

Publication number Publication date
AU5769096A (en) 1996-11-29
WO1996036881A2 (en) 1996-11-21
GB9509844D0 (en) 1995-07-12

Similar Documents

Publication Publication Date Title
AU3538195A (en) Assays and devices for the detection of extrahepatic biliary atresia
WO2000021987A3 (en) A method of identifying modulators of cell surface membrane receptors useful in the treatment of disease
AU2610101A (en) Methods and systems for monitoring intracellular binding reactions
MX9803774A (en) Method for quantification of biological material in a sample.
AU5690694A (en) Human cyclooxygenase-2cdna and assay for evaluating inhibition of cyclooxygenase-2
NZ306051A (en) Testing using electrochemiluminescence
AU7974987A (en) Immunoassay based on two ensyme inhibition system
WO1996036881A3 (en) Screening for inhibitors of tcr-mhc interactions
Yu et al. Antigen-and ionophore-induced signal transduction in rat basophilic leukemia cells involves protein tyrosine phosphorylation.
ATE324462T1 (en) ASSAY FOR DETECTING MODULATORS OF CYTOSKELETAL FUNCTION
WO2005059163A3 (en) Methods, compositions, and kits for analysis of enzyme activity in cells
NO972707L (en) Method of modulating LC132 (opioid-like) receptor function
CA2204305A1 (en) Apparatus and method for determination of urine color
MX9709360A (en) Method for the detection of compounds that modulate the effects of the obese protein.
Abgrall et al. Variation of Weddell seal (Leptonychotes weddellii) underwater vocalizations over mesogeographic ranges
WO2000077241A3 (en) Heparanase assay
ATE333511T1 (en) ANALYTICAL SYSTEM BASED ON SPORE GERMINATION
ATE223045T1 (en) INHIBITION OF AMULIN RELEASE
WO1999019506A3 (en) Method for identifying binding ligands to receptors
WO2002037077A3 (en) Screening methods based on transcriptional regulation analysis system using transformed cell lines
WO1999055901A3 (en) Screening assay for identifying human purinoreceptor ligands
WO2003102154A3 (en) Improved receptor detection
NZ335489A (en) Method for the detection of compounds that modulate the effects of the obese (OB) protein
AU6647490A (en) Enzyme assay and assay kit to measure cellular activation
Ashley et al. Evidence for a K+ channel in bovine chromaffin granule membranes: single-channel properties and possible bioenergetic significance

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BB BG BR BY CA CH CN CZ DE DK EE ES FI GB GE HU IS JP KE KG KP KR KZ LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK TJ TM TR TT UA UG US UZ VN AM AZ BY KG KZ MD RU TJ TM

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): KE LS MW SD SZ UG AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN

AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BB BG BR BY CA CH CN CZ DE DK EE ES FI GB GE HU IS JP KE KG KP KR KZ LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK TJ TM TR TT UA UG US UZ VN AM AZ BY KG KZ MD RU

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): KE LS MW SD SZ UG AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN

121 Ep: the epo has been informed by wipo that ep was designated in this application
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase