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WO1996036717A3 - Dna sequences encoding a lycopene cyclase, antisense sequences derived therefrom and their use for the modification of carotenoids levels in plants - Google Patents

Dna sequences encoding a lycopene cyclase, antisense sequences derived therefrom and their use for the modification of carotenoids levels in plants Download PDF

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Publication number
WO1996036717A3
WO1996036717A3 PCT/EP1996/002101 EP9602101W WO9636717A3 WO 1996036717 A3 WO1996036717 A3 WO 1996036717A3 EP 9602101 W EP9602101 W EP 9602101W WO 9636717 A3 WO9636717 A3 WO 9636717A3
Authority
WO
WIPO (PCT)
Prior art keywords
plants
modification
derived therefrom
lycopene cyclase
dna sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP1996/002101
Other languages
French (fr)
Other versions
WO1996036717A2 (en
Inventor
Marcel Kuntz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Original Assignee
Centre National de la Recherche Scientifique CNRS
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Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS filed Critical Centre National de la Recherche Scientifique CNRS
Priority to AU58977/96A priority Critical patent/AU5897796A/en
Publication of WO1996036717A2 publication Critical patent/WO1996036717A2/en
Publication of WO1996036717A3 publication Critical patent/WO1996036717A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention relates to DNA constructs comprising a DNA sequence homologous to some or all of a sequence encoding a lycopene cyclase, and to their use for modifying carotenoids levels in plants.
PCT/EP1996/002101 1995-05-17 1996-05-17 Dna sequences encoding a lycopene cyclase, antisense sequences derived therefrom and their use for the modification of carotenoids levels in plants Ceased WO1996036717A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU58977/96A AU5897796A (en) 1995-05-17 1996-05-17 Dna sequences encoding a lycopene cyclase, antisense sequenc es derived therefrom and their use for the modification of c arotenoids levels in plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP95401151 1995-05-17
EP95401151.6 1995-05-17

Publications (2)

Publication Number Publication Date
WO1996036717A2 WO1996036717A2 (en) 1996-11-21
WO1996036717A3 true WO1996036717A3 (en) 1996-12-27

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1996/002101 Ceased WO1996036717A2 (en) 1995-05-17 1996-05-17 Dna sequences encoding a lycopene cyclase, antisense sequences derived therefrom and their use for the modification of carotenoids levels in plants

Country Status (2)

Country Link
AU (1) AU5897796A (en)
WO (1) WO1996036717A2 (en)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6872815B1 (en) 2000-10-14 2005-03-29 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
US6972351B2 (en) 1996-08-09 2005-12-06 Calgene Llc Methods for producing carotenoid compounds and specialty oils in plant seeds
US7067647B2 (en) 1999-04-15 2006-06-27 Calgene Llc Nucleic acid sequences to proteins involved in isoprenoid synthesis
US7112717B2 (en) 2002-03-19 2006-09-26 Monsanto Technology Llc Homogentisate prenyl transferase gene (HPT2) from arabidopsis and uses thereof
US7161061B2 (en) 2001-05-09 2007-01-09 Monsanto Technology Llc Metabolite transporters
US7230165B2 (en) 2002-08-05 2007-06-12 Monsanto Technology Llc Tocopherol biosynthesis related genes and uses thereof
US7238855B2 (en) 2001-05-09 2007-07-03 Monsanto Technology Llc TyrA genes and uses thereof
US7244877B2 (en) 2001-08-17 2007-07-17 Monsanto Technology Llc Methyltransferase from cotton and uses thereof
US7262339B2 (en) 2001-10-25 2007-08-28 Monsanto Technology Llc Tocopherol methyltransferase tMT2 and uses thereof

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5792903A (en) * 1993-10-25 1998-08-11 Yissum Research Development Company Of Hebrew University Of Jerusalem Lycopene cyclase gene
US6653530B1 (en) 1998-02-13 2003-11-25 Calgene Llc Methods for producing carotenoid compounds, tocopherol compounds, and specialty oils in plant seeds
AU3749199A (en) * 1998-04-24 1999-11-16 E.I. Du Pont De Nemours And Company Carotenoid biosynthesis enzymes
BR0007128A (en) * 1998-06-02 2001-07-17 Univ Maryland Genes for the biosynthesis and metabolism of carotenoids and their application method
US6252141B1 (en) * 1998-08-14 2001-06-26 Yissum Research And Development Company Of The Hebrew University Of Jerusalem Tomato gene B polynucleotides coding for lycopene cyclase
US6232530B1 (en) * 1998-11-30 2001-05-15 University Of Nevada Marigold DNA encoding beta-cyclase
DE19909637A1 (en) * 1999-03-05 2000-09-07 Peter Beyer Process for improving the agronomic value and nutritional properties of plants
AR030124A1 (en) 2000-08-07 2003-08-13 Monsanto Technology Llc GENES OF THE METHYL-D-ERITRITOL PHOSPHATE VIA
JP2006508681A (en) * 2002-12-06 2006-03-16 デル・モンテ・フレッシュ・プロデュース・カンパニー Transgenic pineapple plant with modified carotenoid level and method for producing the same

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0393690A1 (en) * 1989-04-21 1990-10-24 Kirin Beer Kabushiki Kaisha DNA sequences useful for the synthesis of carotenoids
WO1991009128A1 (en) * 1989-12-13 1991-06-27 Imperial Chemical Industries Plc Dna, constructs, cells and plants derived therefrom
WO1991013078A1 (en) * 1990-03-02 1991-09-05 Amoco Corporation Biosynthesis of carotenoids in genetically engineered hosts
JPH07155189A (en) * 1993-10-25 1995-06-20 Yissum Res Dev Co Of Hebrew Univ Of Jerusalem Lycopene cyclase gene
WO1995023863A1 (en) * 1994-03-01 1995-09-08 Centre National De La Recherche Scientifique (Cnrs) Dna constructs, cells and plants derived therefrom
WO1996028014A1 (en) * 1995-03-07 1996-09-19 Yissum Research Development Company Of The Hebrew University Of Jerusalem Lycopene cyclase gene

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0393690A1 (en) * 1989-04-21 1990-10-24 Kirin Beer Kabushiki Kaisha DNA sequences useful for the synthesis of carotenoids
WO1991009128A1 (en) * 1989-12-13 1991-06-27 Imperial Chemical Industries Plc Dna, constructs, cells and plants derived therefrom
WO1991013078A1 (en) * 1990-03-02 1991-09-05 Amoco Corporation Biosynthesis of carotenoids in genetically engineered hosts
JPH07155189A (en) * 1993-10-25 1995-06-20 Yissum Res Dev Co Of Hebrew Univ Of Jerusalem Lycopene cyclase gene
WO1995023863A1 (en) * 1994-03-01 1995-09-08 Centre National De La Recherche Scientifique (Cnrs) Dna constructs, cells and plants derived therefrom
WO1996028014A1 (en) * 1995-03-07 1996-09-19 Yissum Research Development Company Of The Hebrew University Of Jerusalem Lycopene cyclase gene

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
BOUVIER, F., ET AL.: "Xanthophyll biosynthesis in chromoplasts: isolation and molecular cloning of an enzyme catalyzing the conversion of 5,6-epoxycarotenoid into ketocarotenoid", PLANT JOURNAL, vol. 6, July 1994 (1994-07-01), pages 45 - 54, XP002017248 *
BRAMLEY, P., ET AL.: "BIOCHEMICAL CHARACTERIZATION OF TRANSGENIC TOMATO PLANTS IN WHICH CAROTENOID SYNTHESIS HAS BEEN INHIBITED THROUGH THE EXPRESSION OF ANTISENSE RNA TO PTOM5", THE PLANT JOURNAL, vol. 2, no. 3, pages 343 - 349, XP002017201 *
CAMARA B., ET AL.: "DEMONSTRATION AND SOLUBILIZATION OF LYCOPENE CYCLASE FROM CAPSICUM CHROMOPLAST MEMBRANES", PLANT PHYSIOL (BETHESDA) 80 (1). 1986. 172-174., XP002017199 *
DATABASE WPI Section Ch Week 9533, Derwent World Patents Index; Class C06, AN 95-250738, XP002017205 *
DERUÈRE. J., ET AL.: "Structure and expression of two plant genes encoding chromoplast-specific proteins: occurrence of partially spliced transcripts", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 199, no. 3, 1994, pages 1144 - 1150, XP002017485 *
EMBL SEQUENCE DATABASE ACC. NO. L40176, REL.43, 15-APR-1995. ARABIDOPSIS THALIANA LYCOPENE CYCLASE (LYC) MRNA, COMPLETE CDS. *
FRAY, R.G., ET AL.: "IDENTIFICATION AND GENETIC ANALYSIS OF NORMAL AND MUTANT PHYTOENE SYNTHASE GENES OF TOMATO BY SEQUENCING, COMPLEMENTATION AND CO-SUPPRESSION", PLANT MOLECULAR BIOLOGY, vol. 22, pages 589 - 602, XP002017200 *
HUGUENEY P., ET AL.: "Metabolism of cyclic carotenoids: A model for the alteration of this biosynthetic pathway in Capsicum annuum chromoplasts.", PLANT JOURNAL 8 (3). 1995. 417-424., September 1995 (1995-09-01), XP002017198 *
MISAWA, N., ET AL.: "EXPRESSION OF AN ERWINIA PHYTOENE DESATURASE GENE NOT OLNLY CONFERS MULTIPLE RESISTANCE TO HERBICIDES INTERFERING EITH CAROTENOID BIOSYNTHESIS BUT ALSO ALTERS XANTHOPHYLL METABOLISM IN TRANSGENIC PLANTS", THE PLANT JOURNAL, vol. 6, no. 4, pages 481 - 489, XP002017203 *
MISAWA, N., ET AL.: "FUNCTIONAL EXPRESSION OF THE ERWINIA UREDOVORA CAROTENOID BIOSYNTHESIS GENE CRTI IN TRANSGENIC PLANTS SHOWING AN INCREASE OF BETA-CAROTENE BIOSYNTHESIS ACTIVITY AND RESISTANCE TO THE BLEACHING HERBICIDE NORFLURAZON", THE PLANT JOURNAL, vol. 4, no. 5, pages 833 - 840, XP002017202 *

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6972351B2 (en) 1996-08-09 2005-12-06 Calgene Llc Methods for producing carotenoid compounds and specialty oils in plant seeds
US7067647B2 (en) 1999-04-15 2006-06-27 Calgene Llc Nucleic acid sequences to proteins involved in isoprenoid synthesis
US7141718B2 (en) 1999-04-15 2006-11-28 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
US7265207B2 (en) 1999-04-15 2007-09-04 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
US6872815B1 (en) 2000-10-14 2005-03-29 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
US7161061B2 (en) 2001-05-09 2007-01-09 Monsanto Technology Llc Metabolite transporters
US7238855B2 (en) 2001-05-09 2007-07-03 Monsanto Technology Llc TyrA genes and uses thereof
US7244877B2 (en) 2001-08-17 2007-07-17 Monsanto Technology Llc Methyltransferase from cotton and uses thereof
US7262339B2 (en) 2001-10-25 2007-08-28 Monsanto Technology Llc Tocopherol methyltransferase tMT2 and uses thereof
US7112717B2 (en) 2002-03-19 2006-09-26 Monsanto Technology Llc Homogentisate prenyl transferase gene (HPT2) from arabidopsis and uses thereof
US7230165B2 (en) 2002-08-05 2007-06-12 Monsanto Technology Llc Tocopherol biosynthesis related genes and uses thereof

Also Published As

Publication number Publication date
WO1996036717A2 (en) 1996-11-21
AU5897796A (en) 1996-11-29

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