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WO1996017945B1 - Reversible nuclear genetic system for male sterility in transgenic plants - Google Patents

Reversible nuclear genetic system for male sterility in transgenic plants

Info

Publication number
WO1996017945B1
WO1996017945B1 PCT/US1995/015229 US9515229W WO9617945B1 WO 1996017945 B1 WO1996017945 B1 WO 1996017945B1 US 9515229 W US9515229 W US 9515229W WO 9617945 B1 WO9617945 B1 WO 9617945B1
Authority
WO
WIPO (PCT)
Prior art keywords
promoter
plant
anther
dna sequence
male sterility
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1995/015229
Other languages
French (fr)
Other versions
WO1996017945A1 (en
Filing date
Publication date
Priority claimed from US08/351,899 external-priority patent/US5750868A/en
Priority to CA002207264A priority Critical patent/CA2207264C/en
Application filed filed Critical
Priority to JP51762796A priority patent/JP3370677B2/en
Priority to HU9701839A priority patent/HU226920B1/en
Priority to EP95940806A priority patent/EP0797675B1/en
Priority to KR1019970703834A priority patent/KR100278819B1/en
Priority to MX9704270A priority patent/MX9704270A/en
Priority to DE69535040T priority patent/DE69535040T2/en
Priority to BR9510456A priority patent/BR9510456A/en
Priority to AU42436/96A priority patent/AU698286B2/en
Priority to NZ297303A priority patent/NZ297303A/en
Publication of WO1996017945A1 publication Critical patent/WO1996017945A1/en
Publication of WO1996017945B1 publication Critical patent/WO1996017945B1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Abstract

Plant development can be altered by transforming a plant with a genetic construct that includes regulatory elements and DNA sequences capable of acting in a fashion to inhibit pollen formation or function, thus rendering the transformed plant reversibly male-sterile. In particular, the present invention relates to the use of dominant negative genes and an anther-specific promoter. Male sterility is reversed by incorporation into a plant of a second genetic construct which represses the dominant negative gene. The invention also relates to novel DNA sequences which exhibit the ability to serve as anther-specific promoters in plants.

Claims

AMENDED CLAIMS
[received by the International Bureau on 18 June 1996 (18.06.96); new claims 29 - 38 added; remaining claims unchanged (1 page)]
29. A method of producing a male sterile plant comprising the steps of : producing a transformed plant comprising a recombinant DNA molecule which comprises (i) a DNA sequence encoding a gene product which inhibits pollen formation or function and (ii) a promoter specific to cells critical to pollen formation or function operatively linked to said DNA sequence encoding said gene product; and growing said plant under conditions such that male sterility is achieved as a result of the expression of said DNA sequence .
30. The method of claim 29 wherein said gene product s a cytotoxin. 31. The method of claim 29 wherein said promoter is an anther-specific promoter.
32. The method of claim 31 wherein said anther- specific promoter is 5126 promoter.
33. The method of claim 29, wherein said DNA sequence encodes an exogenous methylase gene.
34. The method of claim 33, wherein said exogenous methylase gene is the DAM methylase gene.
35. The method of claim 34, wherein said promoter is an anther-specific promoter. 36. The method of claim 35, wherein said anther- specific promoter is 5126 promoter.
37. A plant produced by the method of claim 29.
38. A plant produced by the method of claim 1.
PCT/US1995/015229 1994-12-08 1995-12-07 Reversible nuclear genetic system for male sterility in transgenic plants Ceased WO1996017945A1 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
DE69535040T DE69535040T2 (en) 1994-12-08 1995-12-07 REVERSIBLE CORE GENETIC SYSTEM FOR MALE STERILITY IN TRANSGENIC PLANTS
MX9704270A MX9704270A (en) 1994-12-08 1995-12-07 Reversible nuclear genetic system for male sterility in transgenic plants.
JP51762796A JP3370677B2 (en) 1994-12-08 1995-12-07 A reversible nuclear genetic system for male sterility in transgenic plants
HU9701839A HU226920B1 (en) 1994-12-08 1995-12-07 Reversible nuclear genetic system for male sterility in transgenic plants
EP95940806A EP0797675B1 (en) 1994-12-08 1995-12-07 Reversible nuclear genetic system for male sterility in transgenic plants
KR1019970703834A KR100278819B1 (en) 1994-12-08 1995-12-07 Reversible nuclear genetic system for male sterility in transgenic plants
BR9510456A BR9510456A (en) 1994-12-08 1995-12-07 Process for the production of reversible male sterility in a plant
CA002207264A CA2207264C (en) 1994-12-08 1995-12-07 Reversible nuclear genetic system for male sterility in transgenic plants
NZ297303A NZ297303A (en) 1994-12-08 1995-12-07 Producing reversible male sterility in plants using a reversible nuclear genetic system
AU42436/96A AU698286B2 (en) 1994-12-08 1995-12-07 Reversible nuclear genetic system for male sterility in transgenic plants

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/351,899 US5750868A (en) 1994-12-08 1994-12-08 Reversible nuclear genetic system for male sterility in transgenic plants
US08/351,899 1994-12-08
US08/474,556 1995-06-07
US08/474,556 US5689051A (en) 1994-12-08 1995-06-07 Transgenic plants and DNA comprising anther specific promoter 5126 and gene to achieve male sterility

Publications (2)

Publication Number Publication Date
WO1996017945A1 WO1996017945A1 (en) 1996-06-13
WO1996017945B1 true WO1996017945B1 (en) 1996-07-25

Family

ID=26997302

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/015229 Ceased WO1996017945A1 (en) 1994-12-08 1995-12-07 Reversible nuclear genetic system for male sterility in transgenic plants

Country Status (13)

Country Link
US (5) US5763243A (en)
EP (3) EP1273662B1 (en)
JP (2) JP3370677B2 (en)
AR (1) AR000291A1 (en)
AT (1) ATE329040T1 (en)
AU (1) AU698286B2 (en)
DE (1) DE69535040T2 (en)
ES (1) ES2267105T3 (en)
HU (2) HU227303B1 (en)
MX (1) MX9704270A (en)
NZ (1) NZ297303A (en)
PT (1) PT797675E (en)
WO (1) WO1996017945A1 (en)

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