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WO1996005295A3 - An improved cleaning composition - Google Patents

An improved cleaning composition Download PDF

Info

Publication number
WO1996005295A3
WO1996005295A3 PCT/US1995/010426 US9510426W WO9605295A3 WO 1996005295 A3 WO1996005295 A3 WO 1996005295A3 US 9510426 W US9510426 W US 9510426W WO 9605295 A3 WO9605295 A3 WO 9605295A3
Authority
WO
WIPO (PCT)
Prior art keywords
alpha
amylases
mutant
precursor
amylase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1995/010426
Other languages
French (fr)
Other versions
WO1996005295A2 (en
Inventor
Christopher C Barnett
Colin Mitchinson
Scott D Power
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AU33662/95A priority Critical patent/AU686007B2/en
Application filed by Genencor International Inc filed Critical Genencor International Inc
Priority to MX9700776A priority patent/MX9700776A/en
Priority to EP95930186A priority patent/EP0775201A2/en
Priority to KR1019970700864A priority patent/KR970704872A/en
Priority to NZ291984A priority patent/NZ291984A/en
Priority to BR9508582A priority patent/BR9508582A/en
Priority to FI970563A priority patent/FI970563A7/en
Priority to JP8507603A priority patent/JPH10504197A/en
Publication of WO1996005295A2 publication Critical patent/WO1996005295A2/en
Publication of WO1996005295A3 publication Critical patent/WO1996005295A3/en
Priority to NO970609A priority patent/NO970609L/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/39—Organic or inorganic per-compounds
    • C11D3/3942—Inorganic per-compounds
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/39—Organic or inorganic per-compounds
    • C11D3/3947—Liquid compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases. More particularly preferred are mutant alpha-amylases from Bacillus licheniformis modified at MET197 or MET15 or at TRP138 residues or at equivalent residues of other alpha-amylases from other microbial sources (Bacillus, Aspergillus).
PCT/US1995/010426 1994-08-11 1995-08-09 An improved cleaning composition Ceased WO1996005295A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
JP8507603A JPH10504197A (en) 1994-08-11 1995-08-09 Improved cleaning composition
MX9700776A MX9700776A (en) 1994-08-11 1995-08-09 An improved cleaning composition.
EP95930186A EP0775201A2 (en) 1994-08-11 1995-08-09 An improved cleaning composition
KR1019970700864A KR970704872A (en) 1994-08-11 1995-08-09 Improved cleaning composition
NZ291984A NZ291984A (en) 1994-08-11 1995-08-09 Bleach-containing cleaning composition comprising a mutant alpha-amylase and optionally a mutant protease
AU33662/95A AU686007B2 (en) 1994-08-11 1995-08-09 An improved cleaning composition
FI970563A FI970563A7 (en) 1994-08-11 1995-08-09 Improved cleaning composition
BR9508582A BR9508582A (en) 1994-08-11 1995-08-09 Cleaning composition containing bleaching agent
NO970609A NO970609L (en) 1994-08-11 1997-02-10 Improved purification composition

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28935194A 1994-08-11 1994-08-11
US08/289,351 1994-08-11

Publications (2)

Publication Number Publication Date
WO1996005295A2 WO1996005295A2 (en) 1996-02-22
WO1996005295A3 true WO1996005295A3 (en) 1996-03-28

Family

ID=23111158

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/010426 Ceased WO1996005295A2 (en) 1994-08-11 1995-08-09 An improved cleaning composition

Country Status (15)

Country Link
EP (1) EP0775201A2 (en)
JP (1) JPH10504197A (en)
KR (1) KR970704872A (en)
CN (1) CN1158637A (en)
AU (1) AU686007B2 (en)
BR (1) BR9508582A (en)
CA (1) CA2197203A1 (en)
CO (1) CO4440440A1 (en)
FI (1) FI970563A7 (en)
HU (1) HUT77748A (en)
MX (1) MX9700776A (en)
NO (1) NO970609L (en)
NZ (1) NZ291984A (en)
PL (1) PL318209A1 (en)
WO (1) WO1996005295A2 (en)

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US20020155574A1 (en) 2000-08-01 2002-10-24 Novozymes A/S Alpha-amylase mutants with altered properties
ATE449840T1 (en) 2001-05-15 2009-12-15 Novozymes As ALPHA-AMYLASE VARIANT WITH MODIFIED PROPERTIES
DE102004047777B4 (en) 2004-10-01 2018-05-09 Basf Se Alpha-amylase variants with increased solvent stability, process for their preparation and their use
BRPI0812786A2 (en) * 2007-05-30 2014-10-14 Danisco Us Inc Genencor Div BACILLUS LICHENIFORMIS ALPHA AMYLASE IMPROVED VARIANTS
US7618801B2 (en) 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
US20110150955A1 (en) 2009-12-23 2011-06-23 Shannon Elizabeth Klingman Products and Methods for Reducing Malodor from the Pudendum
EP3798303A1 (en) 2011-06-30 2021-03-31 Novozymes A/S Alpha-amylase-variants
WO2014194034A2 (en) 2013-05-29 2014-12-04 Danisco Us Inc. Novel metalloproteases
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Also Published As

Publication number Publication date
AU3366295A (en) 1996-03-07
CN1158637A (en) 1997-09-03
HUT77748A (en) 1998-07-28
CA2197203A1 (en) 1996-02-22
NZ291984A (en) 1998-04-27
FI970563A0 (en) 1997-02-10
KR970704872A (en) 1997-09-06
NO970609L (en) 1997-03-24
EP0775201A2 (en) 1997-05-28
BR9508582A (en) 1998-06-02
FI970563L (en) 1997-02-10
AU686007B2 (en) 1998-01-29
PL318209A1 (en) 1997-05-26
WO1996005295A2 (en) 1996-02-22
NO970609D0 (en) 1997-02-10
CO4440440A1 (en) 1997-05-07
FI970563A7 (en) 1997-02-10
MX9700776A (en) 1997-05-31
JPH10504197A (en) 1998-04-28

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