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WO1996004392A3 - Transgenic cereal plants - Google Patents

Transgenic cereal plants Download PDF

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Publication number
WO1996004392A3
WO1996004392A3 PCT/US1995/008977 US9508977W WO9604392A3 WO 1996004392 A3 WO1996004392 A3 WO 1996004392A3 US 9508977 W US9508977 W US 9508977W WO 9604392 A3 WO9604392 A3 WO 9604392A3
Authority
WO
WIPO (PCT)
Prior art keywords
meristem
contribute
proembryo
transgenic
sectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1995/008977
Other languages
French (fr)
Other versions
WO1996004392A2 (en
Inventor
Benjamin A Bowen
Keith Lowe
Margot C Ross
Gary A Sandahl
Dwight T Tomes
David D Songstad
William J Gordon-Kamm
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Hi Bred International Inc
Original Assignee
Pioneer Hi Bred International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/483,091 external-priority patent/US5736369A/en
Application filed by Pioneer Hi Bred International Inc filed Critical Pioneer Hi Bred International Inc
Priority to NZ293737A priority Critical patent/NZ293737A/en
Priority to MX9700688A priority patent/MX199137B/en
Priority to AU36247/95A priority patent/AU697373B2/en
Priority to EP95933706A priority patent/EP0772687A2/en
Priority to BR9508341A priority patent/BR9508341A/en
Priority to JP8506538A priority patent/JPH10503374A/en
Publication of WO1996004392A2 publication Critical patent/WO1996004392A2/en
Publication of WO1996004392A3 publication Critical patent/WO1996004392A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cereal-Derived Products (AREA)
  • Noodles (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

To obtain a transgenic cereal plant which is stably transformed, an exposed cereal meristem is subjected to biolistic bombardment in order to target non-differentiated meristem cells for transformation. Immature embryos at the early proembryo, mid proembryo, late proembryo, transitional or early coleoptilar stage are harvested for biolistic bombardment. The meristem tissue or cells fated to contribute to the meristem then are manipulated in order to enlarge transgenic sectors, either through selection and/or through effecting a proliferation from the tissue of shoots or multiple meristems per se. The shoot population thus obtained then is screened, by means of a nonlethal enrichment assay, to identify either chimeric sectors that will contribute to germline transmission, or non-sectored, L2 periclinal chimeras that will by definition transmit to progeny. Increased time in culture, under selection, enhances the prospects for sectoral-to-periclinal conversions, and also selects for L1-to-L2 conversions which, through a shift in position, ultimately contribute to the germline. Transgenic sectors also are stabilized during the step of tillering.
PCT/US1995/008977 1994-07-29 1995-07-26 Transgenic cereal plants Ceased WO1996004392A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
NZ293737A NZ293737A (en) 1994-07-29 1995-07-26 A method of producing transgenic cereal plants
MX9700688A MX199137B (en) 1994-07-29 1995-07-26 Transgenic cereal plants.
AU36247/95A AU697373B2 (en) 1994-07-29 1995-07-26 Transgenic cereal plants
EP95933706A EP0772687A2 (en) 1994-07-29 1995-07-26 Transgenic cereal plants
BR9508341A BR9508341A (en) 1994-07-29 1995-07-26 Process for producing transgenic cereal plants
JP8506538A JPH10503374A (en) 1994-07-29 1995-07-26 Transgenic cereal plants

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US28227094A 1994-07-29 1994-07-29
US08/282,270 1994-07-29
US08/483,091 US5736369A (en) 1994-07-29 1995-06-07 Method for producing transgenic cereal plants
US08/483,091 1995-06-07

Publications (2)

Publication Number Publication Date
WO1996004392A2 WO1996004392A2 (en) 1996-02-15
WO1996004392A3 true WO1996004392A3 (en) 1996-03-28

Family

ID=26961344

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/008977 Ceased WO1996004392A2 (en) 1994-07-29 1995-07-26 Transgenic cereal plants

Country Status (9)

Country Link
EP (1) EP0772687A2 (en)
JP (1) JPH10503374A (en)
AU (1) AU697373B2 (en)
BR (1) BR9508341A (en)
CA (1) CA2195206A1 (en)
HU (1) HUT76841A (en)
MX (1) MX199137B (en)
NZ (1) NZ293737A (en)
WO (1) WO1996004392A2 (en)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6235529B1 (en) 1997-04-29 2001-05-22 The Regents Of The University Of California Compositions and methods for plant transformation and regeneration
US7102056B1 (en) 1997-04-29 2006-09-05 The Regents Of The University Of California Compositions and methods for plant transformation and regeneration
WO1999015003A1 (en) 1997-09-24 1999-04-01 The Regents Of The University Of California Methods and compositions for transformation of cereals using cultured shoot meristematic tissue
ES2276475T5 (en) 1997-09-30 2014-07-11 The Regents Of The University Of California Protein production in plant seeds
US6153811A (en) * 1997-12-22 2000-11-28 Dekalb Genetics Corporation Method for reduction of transgene copy number
US6096546A (en) * 1998-01-30 2000-08-01 Board Of Trustees, Rutgers, The State University Of New Jersey Methods for recovering polypeptides from plants and portions thereof
US6100092A (en) 1998-06-15 2000-08-08 Board Of Trustees, Rutgers The State University Of New Jersey Materials and methods for amplifying polynucleotides in plants
CN1426476A (en) * 2000-02-25 2003-06-25 阿维斯塔金格兰技术有限公司 Method for the preparation of genetically modified pearl barnyardgrass by Agrobacterium and biolistic transformation
AU2001255050B2 (en) * 2000-02-25 2005-07-14 Avestha Gengraine Technologies Pvt. Ltd. A process for generating genetically modified pearl millet through agrobacteriumand biolistic transformation
US6544789B1 (en) 2000-03-13 2003-04-08 Board Of Trustees, Rutgers, The State University Of New Jersey Phosphorus-controllable recombinant expression of polypeptides in plants
IT1317038B1 (en) * 2000-06-05 2003-05-26 Vitroplant Vivai Di Zuccherell METHOD FOR THE REGENERATION OF PLANTS AND ITS USES FOR THE MULTIPLICATION AND / OR TRANSFORMATION OF PLANTS.
WO2003007698A2 (en) 2001-07-19 2003-01-30 Monsanto Technology Llc A novel method for the production of transgenic plants
KR20030010234A (en) * 2001-07-26 2003-02-05 권오경 Differential signal receiver circuitry
DE10201637A1 (en) * 2002-01-17 2003-08-07 Univ Albert Ludwigs Freiburg Preparing maize callus cultures, useful for regeneration of whole plants, comprises recovering coleoptile leaf tissue of seedlings and inducing callus formation by culturing in presence of proline, silver nitrate and auxins
US7429691B2 (en) 2002-09-03 2008-09-30 The Regents Of The University Of California Methods and compositions for transformation and regeneration of maize
AU2003293195A1 (en) 2002-12-23 2004-07-29 Vical Incorporated Method for freeze-drying nucleic acid/block copolymer/cationic surfactant complexes
US7381422B2 (en) 2002-12-23 2008-06-03 Vical Incorporated Method for producing sterile polynucleotide based medicaments
CA2682095C (en) 2007-03-29 2014-06-03 International Flower Developments Proprietary Limited Method for producing transgenic surface layer chimeric plant
US10214746B2 (en) 2013-10-04 2019-02-26 Dow Agrosciences Llc Soybean transformation method
JPWO2015133652A1 (en) * 2014-03-06 2017-04-06 国立研究開発法人理化学研究所 Plant transformation methods
US11499158B2 (en) 2016-05-13 2022-11-15 Kaneka Corporation Method for modifying plant
WO2017195905A1 (en) * 2016-05-13 2017-11-16 株式会社カネカ Method for creating transformed plant
EP3456825A4 (en) * 2016-05-13 2020-03-18 Kaneka Corporation METHOD FOR PLANT GENE EDITING

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0290395A2 (en) * 1987-05-05 1988-11-09 Sandoz Ag Plant tissue transformation
EP0331083A2 (en) * 1988-03-02 1989-09-06 Schweizerische Eidgenossenschaft Eidgenössische Technische Hochschule (Eth) Method for the production of transgenic plants
EP0400553A1 (en) * 1989-05-29 1990-12-05 Schweizerische Eidgenossenschaft Eidgenössische Technische Hochschule (Eth) Method for the production of transgenic plants
DE4013099A1 (en) * 1990-04-25 1991-10-31 Hoechst Ag Transforming immature somatic plant, esp. maize, embryos - by treating, in dry state, with nucleic acid soln., esp. for introducing resistance to phosphinothricin
WO1992020809A1 (en) * 1991-05-15 1992-11-26 Agracetus, Inc. Method of creating a transformed rice plant
US5177010A (en) * 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
US5304719A (en) * 1992-02-03 1994-04-19 Pioneer Hi-Bred International, Inc. Inbred corn line PHT47
WO1995006127A1 (en) * 1993-08-20 1995-03-02 Valtion Teknillinen Tutkimuskeskus Method for the production of transgenic cereals

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5177010A (en) * 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
EP0290395A2 (en) * 1987-05-05 1988-11-09 Sandoz Ag Plant tissue transformation
EP0331083A2 (en) * 1988-03-02 1989-09-06 Schweizerische Eidgenossenschaft Eidgenössische Technische Hochschule (Eth) Method for the production of transgenic plants
EP0400553A1 (en) * 1989-05-29 1990-12-05 Schweizerische Eidgenossenschaft Eidgenössische Technische Hochschule (Eth) Method for the production of transgenic plants
DE4013099A1 (en) * 1990-04-25 1991-10-31 Hoechst Ag Transforming immature somatic plant, esp. maize, embryos - by treating, in dry state, with nucleic acid soln., esp. for introducing resistance to phosphinothricin
WO1992020809A1 (en) * 1991-05-15 1992-11-26 Agracetus, Inc. Method of creating a transformed rice plant
US5304719A (en) * 1992-02-03 1994-04-19 Pioneer Hi-Bred International, Inc. Inbred corn line PHT47
WO1995006127A1 (en) * 1993-08-20 1995-03-02 Valtion Teknillinen Tutkimuskeskus Method for the production of transgenic cereals

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BILANG, R., ET AL.: "Transient gene expression in vegetative shot apical meristems of wheat after ballistic microtargeting", THE PLANT JOURNAL, vol. 4, no. 4, pages 735 - 744 *
GAMBLEY, R.L., ET AL.: "Microprojectile transformation of sugarcane meristems and regeneration of shoots expressing beta-glucuronidase", PLANT CELL REPORTS, vol. 12, pages 343 - 346 *
GOULD, J., ET AL.: "Transformation of Zea mays L. using Agrobacterium tumefaciens and the shoot apex", PLANT PHYSIOLOGY, vol. 95, pages 426 - 434 *
LOWE K., ET AL.: "Germline transformation of maize following manipulation of chimeric shoot meristems.", BIO-TECHNOLOGY (NEW YORK) 13 (7). 1995. 677-682. *
LUSARDI, M.C., ET AL.: "An approach towards genetically engineered cell fate mapping in maize using the Lc gene as a visible marker: transactivation capacity of Lc vectors in differentiated maize cells and microinjection of Lc vectors into somatic embryos and shoot apical meristems", THE PLANT JOURNAL, vol. 5, no. 4, pages 571 - 582 *
MCCABE, D.E., ET AL.: "Stable transformation of soybean (Glycine max) by particle acceleration", BIOTECHNOLOGY, vol. 6, pages 923 - 926 *
MCCABE, D.E., ET AL.: "Transformation of elite cotton cultivars via particle bombardment of meristems", BIOTECHNOLOGY, vol. 11, pages 596 - 598 *
RITALA, A., ET AL.: "Fertile transgenic barley by particle bombardment of immature embryos", PLANT MOLECULAR BIOLOGY, vol. 24, pages 317 - 325 *

Also Published As

Publication number Publication date
CA2195206A1 (en) 1996-02-15
JPH10503374A (en) 1998-03-31
WO1996004392A2 (en) 1996-02-15
BR9508341A (en) 1997-08-12
EP0772687A2 (en) 1997-05-14
MX9700688A (en) 1998-01-31
HUT76841A (en) 1997-11-28
MX199137B (en) 2000-10-18
JPH10503374A5 (en) 2001-12-18
AU3624795A (en) 1996-03-04
NZ293737A (en) 1998-07-28
AU697373B2 (en) 1998-10-01

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