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WO1996003511A3 - Procede de production d'acides gras ou derives a partir de plantes oleagineuses - Google Patents

Procede de production d'acides gras ou derives a partir de plantes oleagineuses Download PDF

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Publication number
WO1996003511A3
WO1996003511A3 PCT/FR1995/000957 FR9500957W WO9603511A3 WO 1996003511 A3 WO1996003511 A3 WO 1996003511A3 FR 9500957 W FR9500957 W FR 9500957W WO 9603511 A3 WO9603511 A3 WO 9603511A3
Authority
WO
WIPO (PCT)
Prior art keywords
fatty acids
derivatives
lipase
oil plants
lipids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR1995/000957
Other languages
English (en)
Other versions
WO1996003511A2 (fr
Inventor
Gilbert Alibert
Zephirin Mouloungui
Alain Boudet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut National Polytechnique de Toulouse INPT
Original Assignee
Institut National Polytechnique de Toulouse INPT
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut National Polytechnique de Toulouse INPT filed Critical Institut National Polytechnique de Toulouse INPT
Priority to AU29849/95A priority Critical patent/AU2984995A/en
Priority to US08/776,210 priority patent/US5942659A/en
Priority to EP95925897A priority patent/EP0770134A2/fr
Publication of WO1996003511A2 publication Critical patent/WO1996003511A2/fr
Publication of WO1996003511A3 publication Critical patent/WO1996003511A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6418—Fatty acids by hydrolysis of fatty acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Fats And Perfumes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de production d'acides gras ou de dérivés d'acides gras à partir de plantes oléagineuses. Ce procédé est caractérisé en ce qu'on produit des plantes oléagineuses transgéniques possédant, d'une part, au moins un gène codant pour une enzyme lipase, dit gène de lipase, d'autre part, associé à ce gène de lipase, un promoteur permettant une expression dudit gène soit dans des compartiments différents des compartiments d'accumulation des lipides, soit sur induction exogène, on recueille les graines ou fruits contenant les lipides des plantes, on les broie le cas échéant après traitement inducteur de façon à mettre en contact lipides et lipase, on laisse incuber l'ensemble pour engendrer une hydrolyse enzymatique des lipides et on extrait les acides gras ou dérivés.
PCT/FR1995/000957 1994-07-25 1995-07-18 Procede de production d'acides gras ou derives a partir de plantes oleagineuses Ceased WO1996003511A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU29849/95A AU2984995A (en) 1994-07-25 1995-07-18 Method for producing fatty acids or derivatives thereof from oil plants
US08/776,210 US5942659A (en) 1994-07-25 1995-07-18 Process for producing fatty acids or derivatives thereof from oleaginous plants
EP95925897A EP0770134A2 (fr) 1994-07-25 1995-07-18 Procede de production d'acides gras ou derives a partir de plantes oleagineuses

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9409272A FR2722798B1 (fr) 1994-07-25 1994-07-25 1procede de production d'acides gras2plantes oleagineuses
FR94/09272 1994-07-25

Publications (2)

Publication Number Publication Date
WO1996003511A2 WO1996003511A2 (fr) 1996-02-08
WO1996003511A3 true WO1996003511A3 (fr) 1996-04-25

Family

ID=9465782

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR1995/000957 Ceased WO1996003511A2 (fr) 1994-07-25 1995-07-18 Procede de production d'acides gras ou derives a partir de plantes oleagineuses

Country Status (6)

Country Link
US (1) US5942659A (fr)
EP (1) EP0770134A2 (fr)
AU (1) AU2984995A (fr)
CA (1) CA2195560A1 (fr)
FR (1) FR2722798B1 (fr)
WO (1) WO1996003511A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2733249B1 (fr) * 1995-04-20 1997-06-06 Biocem Lipase gastrique de chien recombinante et polypeptides derives produits par les plantes, leurs procedes d'obtention et leurs utilisations
FR2754827B1 (fr) * 1996-10-17 1998-12-24 Biocem Lipases pancreatiques et/ou colipases recombinantes et polypeptides dervies produits par les plantes, leurs procedes d'obtention et leurs utilisations
DE19731990A1 (de) * 1997-07-25 1999-01-28 Studiengesellschaft Kohle Mbh Verfahren zur Herstellung und Identifizierung von neuen Hydrolasen mit verbesserten Eigenschaften
FR2769320B1 (fr) * 1997-10-03 2002-03-29 Total Raffinage Distrib Procede de production d'acides gras ramifies au moyen de plantes genetiquement modifiees
US7166766B1 (en) 2000-04-03 2007-01-23 Total Raffinage Distribution S.A. Method for producing branched fatty acids using genetically modified plants
KR20020010119A (ko) 1999-02-16 2002-02-02 세네스코 인코포레이티드 식물 리파제를 엔코딩하는 디엔에이, 형질전환된 식물과식물 노화를 조절하기 위한 방법
GB0022236D0 (en) * 2000-09-11 2000-10-25 Pinet Aylette Partially hydrolysed protein nutrient supplement
MXPA06009411A (es) * 2004-02-20 2007-03-29 Pioneer Hi Bred Int Lipasas y metodos de uso.
US20060212964A9 (en) * 2004-02-20 2006-09-21 Pioneer Hi-Bred International, Inc. Methods for enhancing insect resistance in plants
WO2011008058A2 (fr) * 2009-07-17 2011-01-20 한국과학기술원 Procédé de fabrication d'ester alkyliques d'acides gras à l'aide de microorganismes ayant une aptitude à la production d'huile

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0427309A2 (fr) * 1989-11-09 1991-05-15 Unilever N.V. Procédé pour la préparation de graisses obtenues à partir de cruciferae
WO1991006661A1 (fr) * 1989-11-03 1991-05-16 Opta Food Ingredients, Inc. Production in situ de monoglycerides et de diglycerides catalysee par lipase
EP0449376A2 (fr) * 1990-03-23 1991-10-02 Gist-Brocades N.V. Production d'enzymes dans des semences et leur utilisation
WO1992001042A1 (fr) * 1990-07-06 1992-01-23 Novo Nordisk A/S Plantes transgeniques exprimant des enzymes industrielles
WO1992005249A1 (fr) * 1990-09-13 1992-04-02 Novo Nordisk A/S Variantes lipasiques
WO1993003161A1 (fr) * 1990-10-22 1993-02-18 Biosource Genetics Corporation Acides nucleiques viraux recombines de plantes

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006661A1 (fr) * 1989-11-03 1991-05-16 Opta Food Ingredients, Inc. Production in situ de monoglycerides et de diglycerides catalysee par lipase
EP0427309A2 (fr) * 1989-11-09 1991-05-15 Unilever N.V. Procédé pour la préparation de graisses obtenues à partir de cruciferae
EP0449376A2 (fr) * 1990-03-23 1991-10-02 Gist-Brocades N.V. Production d'enzymes dans des semences et leur utilisation
WO1992001042A1 (fr) * 1990-07-06 1992-01-23 Novo Nordisk A/S Plantes transgeniques exprimant des enzymes industrielles
WO1992005249A1 (fr) * 1990-09-13 1992-04-02 Novo Nordisk A/S Variantes lipasiques
WO1993003161A1 (fr) * 1990-10-22 1993-02-18 Biosource Genetics Corporation Acides nucleiques viraux recombines de plantes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
PEN, J., ET AL.: "PRODUCTION OF ACTIVE ALPHA-AMYLASE IN PLANTS AND THE APPLICATION IN STARCH LIQUEFACTION", JOURNAL OF CELLULAR BIOCHEMISTRY SUPPLEMENT, vol. 16F, pages 227 *
PEN, J., ET AL.: "PRODUCTION OF ACTIVE BACILLUS LICHENIFORMIS ALPHA-AMYLASEIN TOBACCO AND ITS APPLICATION IN STARCH LIQUEFACTION", BIOTECHNOLOGY, vol. 10, no. 3, NEW YORK US, pages 292 - 296 *

Also Published As

Publication number Publication date
WO1996003511A2 (fr) 1996-02-08
CA2195560A1 (fr) 1996-02-08
EP0770134A2 (fr) 1997-05-02
FR2722798B1 (fr) 1996-09-13
US5942659A (en) 1999-08-24
FR2722798A1 (fr) 1996-01-26
AU2984995A (en) 1996-02-22

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