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WO1996000788B1 - Transgenic plants with an increased secondary substance content - Google Patents

Transgenic plants with an increased secondary substance content

Info

Publication number
WO1996000788B1
WO1996000788B1 PCT/DE1995/000850 DE9500850W WO9600788B1 WO 1996000788 B1 WO1996000788 B1 WO 1996000788B1 DE 9500850 W DE9500850 W DE 9500850W WO 9600788 B1 WO9600788 B1 WO 9600788B1
Authority
WO
WIPO (PCT)
Prior art keywords
transgenic plant
plant according
dna sequence
polypeptide
heterologous dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE1995/000850
Other languages
German (de)
French (fr)
Other versions
WO1996000788A1 (en
Filing date
Publication date
Priority claimed from DE4423022A external-priority patent/DE4423022C1/en
Application filed filed Critical
Priority to JP8502714A priority Critical patent/JPH10502244A/en
Priority to AU28792/95A priority patent/AU2879295A/en
Priority to EP95924162A priority patent/EP0767838A1/en
Publication of WO1996000788A1 publication Critical patent/WO1996000788A1/en
Publication of WO1996000788B1 publication Critical patent/WO1996000788B1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Abstract

Transgenic plants with at least one heterologous DNA sequence have an increased secondary substance content. Also disclosed is a process for producing the same and their use in forestry, grazing lands, meadows, ornamental and useful plant cultivation. In particular, the heterologous DNA sequence codes for a polypeptide with enzymatic activity that enzymatically generates at least one secondary substance in sufficient concentration to have an anti-viral, bactericide, fungicide, insecticide and/or insect repellent effect.

Claims

GEÄNDERTE ANSPRÜCHE[beim Internationalen Büro am 11. Januar 1996 (11.01.96) eingegangen; ursprüngliche Ansprüche 1 und 2 durch neuer Anspruch 1 ersetzt;Ansprüche 3-12 unverändert aber umnumeriert als Ansprüche 2-11 (2 Seiten)] AMENDED CLAIMS [received by the International Bureau on 11 January 1996 (11.01.96); original claims 1 and 2 replaced by new claim 1; claims 3-12 unchanged but renumbered as claims 2-11 (2 pages)] 1. Transgene Pflanze mit mindestens einer heterologen DNA-Sequenz, die für mindestens ein Polypeptid mit enzymatisc er Aktivität kodiert, worin das Polypeptid exprimiert wird, in enzymatisch aktiver Form vorliegt und mindestens einen Sekundärstoff in einer antiviral und/oder bakterizid und/oder fungizid und/oder insektizid und/oder als Repellent wirkenden Konzentration enzymatisch erzeugt, wobei die heterologe DNA-Sequenz aus Bakterien stammt.A transgenic plant having at least one heterologous DNA sequence encoding at least one polypeptide having enzymatic activity wherein the polypeptide is expressed is in enzymatically active form and at least one ancillary substance in an antiviral and / or bactericidal and / or fungicidal and / or insecticidally and / or acting as a repellent concentration produced enzymatically, wherein the heterologous DNA sequence derived from bacteria. 2. Transgene Pflanze nach Anspruch 1, wobei die hete¬ rologe DNA-Sequenz aus Escherichia coli stammt.2. Transgenic plant according to claim 1, wherein the hete rologous DNA sequence is derived from Escherichia coli. 3. Transgene Pflanze nach Anspruch 1 oder 2, wobei die heterologe DNA-Sequenz die folgende Sequenz enthält:3. A transgenic plant according to claim 1 or 2, wherein the heterologous DNA sequence contains the following sequence: ATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCC CTGGATCCGCAACTGCTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAA CAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAA ATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTTACTGGTTACGTGAAATTTTG TTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTA AGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTC ACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGG CGACGTTCCCGCCTGCGATTAAGCGGTAAACCGCTGTTGCTAACAGAACTGTTTTTACCG GCGTCACCGTTGTACATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCC CTGGATCCGCAACTGCTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAA CAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAA ATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTTACTGGTTACGTGAAATTTTG TTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTA AGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTC ACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGG CGACGTTCCCGCCTGCGATTAAGCGGTAAACCGCTGTTGCTAACAGAACTGTTTTTACCG GCGTCACCGTTGTAC 4. Transgene Pflanze nach einem der Ansprüche 1 bis 3, wobei das Polypeptid das Enzym Chorismat-Pyruvat-Lyase oder das Enzym Isochorismat-Pyruvat-Lyase (= Salicylat-Synthase) is .4. Transgenic plant according to one of claims 1 to 3, wherein the polypeptide is the enzyme chorismate pyruvate lyase or the enzyme isochorismate pyruvate lyase (= salicylate synthase). 5. Transgene Pflanze nach einem der Ansprüche i bis 4, wobei der Sekundärstoff ein pflanzeneigener Stoff ist. 5. Transgenic plant according to one of claims i to 4, wherein the secondary substance is a plant-own substance. 6. Transgene Pflanze nach einem der Ansprüche 1 bis 5, wobei der Sekundärstof ein Phenolderivat ist.6. Transgenic plant according to one of claims 1 to 5, wherein the Sekundärstof is a phenol derivative. 7. Transgene Pflanze nach einem der Ansprüche 1 bis 6, wobei der Sekundärstoff p-Hydroxybenzoesäure und/oder deren Derivat (e) ist.7. A transgenic plant according to any one of claims 1 to 6, wherein the secondary substance is p-hydroxybenzoic acid and / or its derivative (s). 8. Transgene Pflanze nach einem der Ansprüche 1 bis 7, wobei die Konzentration des Sekundärstoffs mindestens das lOfache bezogen auf die Konzentration des Sekundärstof s im Wildtyp beträgt.8. Transgenic plant according to one of claims 1 to 7, wherein the concentration of the secondary substance is at least 10 times based on the concentration of Sekundärstof s in the wild type. 9. Verfahren zur Herstellung einer transgenen Pflanze nach einem der Anspruch 1 bis 8, worin eine Pflanzenzelle durch stabile Integration der heterologen DNA-Sequenz in das genetische Material transformiert wird und die transformierte Pflanzenzelle zur transgenen Pflanze re¬ generiert wird.9. A method for producing a transgenic plant according to any one of claims 1 to 8, wherein a plant cell is transformed by stable integration of the heterologous DNA sequence into the genetic material and the transformed plant cell to the transgenic plant re¬ generated. 10. Verwendung der transgenen Pflanze nach einem der Ansprüche 1 bis 8 als pathogenresistente und/oder gegen Freßfeinde und/oder Parasiten resistente Pflanze im Wald- , Weide-, Wiesen-, Zierpflanzen- oder Nutzpflanzenbau.10. Use of the transgenic plant according to one of claims 1 to 8 as a pathogen-resistant and / or against predators and / or parasites resistant plant in forestry, pasture, meadow, ornamental plants or crops. 11. Verwendung einer heterologen DNA-Sequenz, die für mindestens ein Polypeptid mit enzymatischer Aktivität kodiert, zur Herstellung einer transgenen Pflanze mit mindestens einem durch das Polypeptid erzeugten pflanzeneigenen Sekundärstoff in einer antiviral und/oder bakterizid und/oder fungizid und/oder insektizid und/oder als Repellent wirkenden Konzentration. 11. Use of a heterologous DNA sequence which codes for at least one polypeptide having enzymatic activity, for producing a transgenic plant having at least one plant-derived secondary substance produced by the polypeptide in an antiviral and / or bactericidal and / or fungicidal and / or insecticidal and / or or as a repellent concentration.
PCT/DE1995/000850 1994-06-30 1995-06-28 Transgenic plants with an increased secondary substance content Ceased WO1996000788A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP8502714A JPH10502244A (en) 1994-06-30 1995-06-28 Transgenic plants containing increased secondary substances
AU28792/95A AU2879295A (en) 1994-06-30 1995-06-28 Transgenic plants with an increased secondary substance content
EP95924162A EP0767838A1 (en) 1994-06-30 1995-06-28 Transgenic plants with an increased secondary substance content

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4423022A DE4423022C1 (en) 1994-06-30 1994-06-30 Transgenic plants with increased content of resistance factor
DEP4423022.2 1994-06-30

Publications (2)

Publication Number Publication Date
WO1996000788A1 WO1996000788A1 (en) 1996-01-11
WO1996000788B1 true WO1996000788B1 (en) 1996-02-15

Family

ID=6521963

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1995/000850 Ceased WO1996000788A1 (en) 1994-06-30 1995-06-28 Transgenic plants with an increased secondary substance content

Country Status (5)

Country Link
EP (1) EP0767838A1 (en)
JP (1) JPH10502244A (en)
AU (1) AU2879295A (en)
DE (1) DE4423022C1 (en)
WO (1) WO1996000788A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0889691B1 (en) * 1996-03-25 2003-01-29 Proguard, Inc. Use of aromatic aldehydes as pesticides
US6683231B2 (en) * 2000-06-02 2004-01-27 E. I. Du Pont De Nemours And Company High level production of p-hydroxybenzoic acid in green plants
US6642036B2 (en) 2000-07-07 2003-11-04 E. I. Du Pont De Nemours And Company Sinapoylglucose:malate sinapoyltransferase form malate conjugates from benozic acid glucosides
US7122718B2 (en) 2000-07-28 2006-10-17 Dairy Australia Limited Modification of plant resistance to diseases and/or pests
DE10113045A1 (en) * 2001-03-14 2002-09-19 Tares Gmbh Fungicide that inhibits secreted lipolytic enzymes, useful for plant protection and clinical use, e.g. acetylsalicylic acid
US7056742B2 (en) 2003-06-16 2006-06-06 E. I. Du Pont De Nemours And Company High Level production of arbutin in green plants
WO2012063862A1 (en) * 2010-11-10 2012-05-18 グリーンフェノール・高機能フェノール樹脂製造技術研究組合 Coryneform bacterium transformant and method for producing phenol using same
DE102014016774B4 (en) * 2014-11-12 2016-09-29 Eberhard Karls Universität Tübingen Plants with increased resistance to plant pathogens and methods for producing increased pathogen resistance in plants

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57212104A (en) * 1981-06-24 1982-12-27 Hokko Chem Ind Co Ltd Agricultural and horticultural fungicide
DE4107396A1 (en) * 1990-06-29 1992-01-02 Bayer Ag STYLE SYNTHASE GENES FROM VINEYARD
US5306862A (en) * 1990-10-12 1994-04-26 Amoco Corporation Method and composition for increasing sterol accumulation in higher plants
DE4117747A1 (en) * 1991-05-30 1992-12-03 Bayer Ag COFFEOYL COA 3-O-METHYLTRANSFERASE GENES
DE4130986A1 (en) * 1991-09-18 1993-03-25 Bayer Ag PINOSYLVIN SYNTHASE GENES
WO1993015599A1 (en) * 1992-01-31 1993-08-19 Cornell Research Foundation, Inc. Polyphenol oxidase
FI95195C (en) * 1992-02-27 1996-01-10 Kemira Oy preservative Blends
WO1993022441A1 (en) * 1992-04-23 1993-11-11 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Dna sequence of a plant resistance gene
DE4234131C2 (en) * 1992-10-09 1995-08-24 Max Planck Gesellschaft Transgenic pathogen-resistant organism
DE69420957T2 (en) * 1993-04-02 2000-01-13 Pioneer Hi-Bred International, Inc. FIGHT AGAINST DIABROTICA UNDECIMPUNCTATA BY LIMES
US5496732A (en) * 1993-04-30 1996-03-05 The United States Of America As Represented By The Secretary Of Agriculture Enhanced insect resistance in plants genetically engineered with a plant hormone gene involved in cytokinin biosynthesis
DE4334791A1 (en) * 1993-10-13 1995-04-20 Bayer Ag Bibenzyl synthase genes

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