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WO1995033995A1 - Procede et trousse pour detecter des anticorps - Google Patents

Procede et trousse pour detecter des anticorps Download PDF

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Publication number
WO1995033995A1
WO1995033995A1 PCT/US1995/007190 US9507190W WO9533995A1 WO 1995033995 A1 WO1995033995 A1 WO 1995033995A1 US 9507190 W US9507190 W US 9507190W WO 9533995 A1 WO9533995 A1 WO 9533995A1
Authority
WO
WIPO (PCT)
Prior art keywords
kit
platelet
antigens
hla
antibodies
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1995/007190
Other languages
English (en)
Inventor
Michael Moghaddam
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GTI Corp
Original Assignee
GTI Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GTI Corp filed Critical GTI Corp
Publication of WO1995033995A1 publication Critical patent/WO1995033995A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56977—HLA or MHC typing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Definitions

  • the present invention relates to a method and diagnostic kit for detecting platelet
  • HLA human leukocyte-A antigens
  • antigens are often found on membrane glycoproteins present on the surface of the cell membranes.
  • HLA Human Lymphocyte Antigens
  • the blood platelet is one of the four primary elements that are important in
  • Platelets release mediators which promote a number of responses that
  • hematologic disorders such as leukemia, systemic lupus erythematosus or other collagen-vascular
  • HLA alloimmunity a unique immune state
  • HLA alloimmunity ranges between 30 and 70 %.
  • Neonatal alloimmune thrombocytopenic purpura (NATP) and posttransfusion
  • PTP purpura
  • AITP Autoimmune thrombocytopenia
  • the method generally includes placing patient or donor serum into microtiter wells
  • glycoproteins The incubation of the serum with the potential antigens will allow an antibody-
  • Anti-IgG/IgM/lgA phosphatase labelled anti-human globulin reagent(s)
  • microtiter wells are washed to remove any unbound
  • PNPP p-nitrophenyl phosphate
  • the optical density of the color is measured at a wavelength of 410 nm.
  • a kit comprises at least one microtiter plate with wells pre-coated
  • a receptacle is provided for each of the anti-IgG/IgM/IgA reagent(s) and PNPP substrate.
  • a receptacle may also be provided for each of the following: wash solutions, the sodium-hydroxide stop solution, the enzyme substrate buffer, a specimen diluent solution, a positive control and a negative control.
  • the present invention is a solid phase ELISA assay method and kit which is used
  • the method provides for the use of HLA Class I
  • GPIIb/IIIa antigens and platelet glycoproteins GPIIb/IIIa, GPIb/IX, GPla/IIa and GPIV.
  • Blood should be drawn from a donor or patient using aseptic technique and should
  • wash solution combines:
  • the solution stand for about 5-15 minutes, preferably 10 minutes.
  • the microtiter plate should be
  • At least one well of the plate may contain HLA Class I attached to it's wall; at least one well may contain GPIIb/IIIa; at least one well may contain
  • GPIb/IX at least one well may contain GPIa/IIa, and, at least one well may contain GPIV.
  • Each well could include one of the listed antigens or any combination, but, in the preferred
  • the patient samples are diluted along with a negative control and a positive
  • the dilution proceeds as follows: a. Add 140 ⁇ l of a negative sample to 420 ⁇ l of a specimen diluent solution.
  • negative sample is normal blood type AB serum with 0.1% sodium azide added as a preservative.
  • the specimen diluent solution is a phosphate buffered saline (PBS) solution containing bovine
  • the strong positive serum control is serum strongly positive for platelet antigen-antibody reaction; it contains 0.1% sodium azide as a preservative. c. Add 50 ⁇ l of weak positive sample to 150 ⁇ l of the specimen diluent solution. 15 The weak positive serum control is weakly positive for platelet antigen-antibody reaction; it also
  • the plate may be incubated for about 20-40 minutes at 30°C.
  • the substrate buffer has the following ingredients:
  • the absorbance (OD) of each well should be measured at preferably 410nm, using
  • control numbers is the control mean multiplied by 2. Any number greater that the indicated
  • control number shows a positive reaction for each group.
  • the control number refers to the prior
  • the present invention also provides a diagnostic assay for_detection of antibodies for platelet antigens in kit form.
  • kit form typically includes the following items in amounts
  • Solid support a solid support having bound thereto one or more HLA Class I
  • antigens and/or platelet glycoproteins that will specifically react with a human antibody.
  • the solid support is a preformed microtiter plate having one or more HLA Class I antigens or platelet glycoproteins (GPIIb-IIIa, GPIb-IX, Gpla-lla, GPIV) bound to a well surface.
  • HLA Class I antigens or platelet glycoproteins GPIIb-IIIa, GPIb-IX, Gpla-lla, GPIV
  • IgG/IgA/IgM antibodies in solution positive serum control; negative serum control; PNPP;
  • the kit also contains at least one plate sealer and at least one strip frame.
  • Instructions for use are also included. By the term “instructions for use,” it is
  • reagent concentration for at least one assay method, parameters such as the relative amount of reagent and sample to be admixed, maintenance time periods for reagent/sample admixtures, temperature, buffer conditions and the like.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention vise à déterminer dans le sang d'un patient la présence d'anticorps qui sont spécifiques contre les antigènes HLA de la classe I et/ou les glycoprotéines plaquettaires. L'invention décrit un procédé et une trousse pour diagnostiquer des troubles plaquettaires dans un format facile à utiliser. Le procédé et la trousse mettent en ÷uvre un support solide tel que des puits de plaquettes de microtitrage qui sont revêtus des antigènes HLA et glycoprotéiques. Un échantillon sanguin du patient est ajouté aux puits revêtus et mis en réaction, puis la détection spectrophotométrique des anticorps permet un dosage diagnostique.
PCT/US1995/007190 1994-06-06 1995-06-06 Procede et trousse pour detecter des anticorps Ceased WO1995033995A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/254,398 US5514557A (en) 1994-06-06 1994-06-06 Method and kit for detecting antibodies specific for HLA and/or platelet glycoproteins
US08/254,398 1994-06-06

Publications (1)

Publication Number Publication Date
WO1995033995A1 true WO1995033995A1 (fr) 1995-12-14

Family

ID=22964155

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/007190 Ceased WO1995033995A1 (fr) 1994-06-06 1995-06-06 Procede et trousse pour detecter des anticorps

Country Status (2)

Country Link
US (1) US5514557A (fr)
WO (1) WO1995033995A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997044667A3 (fr) * 1996-05-21 1998-03-19 Pasteur Institut Methodes d'utilisation de complexes peptide/complexe majeur d'histocompatibilite pour obtenir ou purifier des cellules T antigene-specifiques et pour stimuler des cellules T
KR100375105B1 (ko) * 2000-09-08 2003-03-08 주식회사 셀텍 광 다이오드를 이용한 질병 및 바이러스 분석장치
US8088586B2 (en) 2000-03-17 2012-01-03 Oxford Radcliffe Hospital Nhs Trust Method of producing a body fluid sample depleted of anti-MHC antibodies
US8609436B2 (en) 2000-03-17 2013-12-17 Guy's & St Thomas' Hospital NHS Trust (“GST”) Method

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5948627A (en) 1997-05-30 1999-09-07 One Lambda Immunobead flow cytometric detection of anti-HLA panel-reactive antibody
US6046013A (en) * 1997-08-01 2000-04-04 Gti Process for identifying specific antibodies associated with HLA
US6623981B2 (en) * 1998-01-27 2003-09-23 Bristol-Myers Squibb Company Detection of patients at risk for developing integrin antagonist/agonist mediated disease states
US20040072262A1 (en) * 2002-10-11 2004-04-15 Montero-Julian Felix A. Methods and systems for detecting MHC class I binding peptides
AU2004206821C1 (en) 2003-01-14 2009-10-01 Gilead Sciences, Inc. Compositions and methods for combination antiviral therapy
EP1692504A4 (fr) * 2003-11-03 2007-06-27 Beckman Coulter Inc Procedes a base de solutions de detection de peptides se fixant au cmh
EP1766393A4 (fr) * 2004-05-07 2008-06-18 Beckman Coulter Inc Système de formation de pont de complexes d'histocompatibilité principale pour détecter une lyse médiée par les lymphocytes t de cellules présentant des antigènes
EP2226332A1 (fr) * 2004-06-17 2010-09-08 Beckman Coulter, Inc. Épitopes de tuberculose par mycobacterium et ses procédés d'utilisation
US7189522B2 (en) 2005-03-11 2007-03-13 Chembio Diagnostic Systems, Inc. Dual path immunoassay device
WO2006098804A2 (fr) 2005-03-11 2006-09-21 Chembio Diagnostic Systems, Inc. Dispositif de dosage immunologique a double trajet
TWI471145B (zh) 2005-06-13 2015-02-01 Bristol Myers Squibb & Gilead Sciences Llc 單一式藥學劑量型
TWI375560B (en) 2005-06-13 2012-11-01 Gilead Sciences Inc Composition comprising dry granulated emtricitabine and tenofovir df and method for making the same
US20070059782A1 (en) * 2005-09-13 2007-03-15 Graham Henry A Magnetic particle tagged blood bank reagents and techniques
US20100022916A1 (en) 2008-07-24 2010-01-28 Javanbakhsh Esfandiari Method and Apparatus for Collecting and Preparing Biological Samples for Testing
US8603835B2 (en) 2011-02-10 2013-12-10 Chembio Diagnostic Systems, Inc. Reduced step dual path immunoassay device and method
SG11201608278WA (en) 2014-04-02 2016-10-28 Chembio Diagnostic Systems Inc Immunoassay utilizing trapping conjugate
US20160116466A1 (en) 2014-10-27 2016-04-28 Chembio Diagnostic Systems, Inc. Rapid Screening Assay for Qualitative Detection of Multiple Febrile Illnesses
TWI672502B (zh) * 2018-10-05 2019-09-21 長庚大學 血小板抗體篩檢及同時進行血小板交叉配對之免疫檢驗方法及檢測裝置
CN112710846A (zh) * 2020-12-16 2021-04-27 江苏伟禾生物科技有限公司 一种测定血小板抗体检测的板式免疫荧光试剂盒及其制备方法
CN113447649B (zh) * 2021-07-01 2022-04-19 浙江大学 一种检测抗粘着斑蛋白-IgG抗体的试剂盒

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4810632A (en) * 1986-12-01 1989-03-07 Scripps Clinic And Research Foundation Cell surface antigen detection method
US5110726A (en) * 1987-08-27 1992-05-05 Board Of Regents, The University Of Texas System Immunoassay for antibodies binding platelets
US5180661A (en) * 1988-03-14 1993-01-19 Rei, Inc. Platelet cross matching
US5231025A (en) * 1989-11-03 1993-07-27 The United States Of America As Represented By The Department Of Health And Human Services Anti-platelet monoclonal antibody
US5292641A (en) * 1991-12-13 1994-03-08 Sangstat Medical Corporation Alloantigen testing by binding assay

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4810632A (en) * 1986-12-01 1989-03-07 Scripps Clinic And Research Foundation Cell surface antigen detection method
US5110726A (en) * 1987-08-27 1992-05-05 Board Of Regents, The University Of Texas System Immunoassay for antibodies binding platelets
US5180661A (en) * 1988-03-14 1993-01-19 Rei, Inc. Platelet cross matching
US5231025A (en) * 1989-11-03 1993-07-27 The United States Of America As Represented By The Department Of Health And Human Services Anti-platelet monoclonal antibody
US5292641A (en) * 1991-12-13 1994-03-08 Sangstat Medical Corporation Alloantigen testing by binding assay

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997044667A3 (fr) * 1996-05-21 1998-03-19 Pasteur Institut Methodes d'utilisation de complexes peptide/complexe majeur d'histocompatibilite pour obtenir ou purifier des cellules T antigene-specifiques et pour stimuler des cellules T
US8088586B2 (en) 2000-03-17 2012-01-03 Oxford Radcliffe Hospital Nhs Trust Method of producing a body fluid sample depleted of anti-MHC antibodies
US8609436B2 (en) 2000-03-17 2013-12-17 Guy's & St Thomas' Hospital NHS Trust (“GST”) Method
KR100375105B1 (ko) * 2000-09-08 2003-03-08 주식회사 셀텍 광 다이오드를 이용한 질병 및 바이러스 분석장치

Also Published As

Publication number Publication date
US5514557A (en) 1996-05-07

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