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WO1995027209A1 - METHODE ET KIT DE DETECTION DE l'ACTIVATION DU RECEPTEUR DE THROMBINE DANS LES PLAQUETTES ET AUTRES CELLULES - Google Patents

METHODE ET KIT DE DETECTION DE l'ACTIVATION DU RECEPTEUR DE THROMBINE DANS LES PLAQUETTES ET AUTRES CELLULES Download PDF

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Publication number
WO1995027209A1
WO1995027209A1 PCT/US1995/003796 US9503796W WO9527209A1 WO 1995027209 A1 WO1995027209 A1 WO 1995027209A1 US 9503796 W US9503796 W US 9503796W WO 9527209 A1 WO9527209 A1 WO 9527209A1
Authority
WO
WIPO (PCT)
Prior art keywords
antibody
peptide fragment
cleaved peptide
thrombin
thrombin receptor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1995/003796
Other languages
English (en)
Inventor
Lawrence Brass
James A. Hoxie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Pennsylvania Penn
Original Assignee
University of Pennsylvania Penn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Pennsylvania Penn filed Critical University of Pennsylvania Penn
Publication of WO1995027209A1 publication Critical patent/WO1995027209A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/974—Thrombin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Méthode de détection de l'activation des cellules induites par la thrombine à l'aide d'un système de détection permettant de déterminer la présence d'un fragment peptide clivé du récepteur de thrombine. Cette méthode permet de diagnostiquer et de traiter les états pathologiques pré-thrombotiques et thrombotiques. L'invention porte également sur les kits de détection utilisés pour l'application de cette méthode.
PCT/US1995/003796 1994-03-31 1995-03-27 METHODE ET KIT DE DETECTION DE l'ACTIVATION DU RECEPTEUR DE THROMBINE DANS LES PLAQUETTES ET AUTRES CELLULES Ceased WO1995027209A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22037594A 1994-03-31 1994-03-31
US08/220,375 1994-03-31

Publications (1)

Publication Number Publication Date
WO1995027209A1 true WO1995027209A1 (fr) 1995-10-12

Family

ID=22823307

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/003796 Ceased WO1995027209A1 (fr) 1994-03-31 1995-03-27 METHODE ET KIT DE DETECTION DE l'ACTIVATION DU RECEPTEUR DE THROMBINE DANS LES PLAQUETTES ET AUTRES CELLULES

Country Status (1)

Country Link
WO (1) WO1995027209A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996010749A1 (fr) * 1994-09-29 1996-04-11 Mount Sinai School Of Medicine Of The City University Of New York Test d'evaluation du blocage des plaquettes sanguines
WO2009098689A1 (fr) * 2008-02-07 2009-08-13 Hadasit Medical Research Services & Development Limited Immunodétection d'un état cancéreux chez un sujet
US8574828B2 (en) 2003-07-08 2013-11-05 Accumetrics, Inc. Controlled platelet activation to monitor therapy of ADP antagonists
US9506938B2 (en) 2003-07-08 2016-11-29 Accumetrics, Inc. Methods for measuring platelet reactivity of individuals treated with drug eluting stents
CN107102134A (zh) * 2017-03-02 2017-08-29 江苏华冠生物技术股份有限公司 一种适用于末梢血检测用的样品稀释液

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992014750A1 (fr) * 1991-02-19 1992-09-03 The Regents Of The University Of California Recepteur de thrombine recombinant et produits pharmaceutiques correspondants
US5256766A (en) * 1991-02-19 1993-10-26 The Regents Of The University Of California Recombinant thrombin receptor and related pharmaceuticals

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992014750A1 (fr) * 1991-02-19 1992-09-03 The Regents Of The University Of California Recepteur de thrombine recombinant et produits pharmaceutiques correspondants
US5256766A (en) * 1991-02-19 1993-10-26 The Regents Of The University Of California Recombinant thrombin receptor and related pharmaceuticals

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BLOOD, Volume 82, Number 7, issued 01 October 1993, K.J. NORTON et al., "Immunologic Analysis of the Cloned Platelet Thrombin Receptor Activation Mechanism: Evidence Supporting Receptor Cleavage, Release of the N-Terminal Peptide and Insertion of the Tethered Ligand Into a Protected Environment", pages 2125-2136. *
CLINICAL CHEMISTRY, Volume 36, Number 8, issued 1990, J.P. GOSLING, "A Decade of Development in Immunoassay Methodology", pages 1408-1427. *
JOURNAL OF BIOLOGICAL CHEMISTRY, Volume 267, Number 20, issued 15 July 1992, L.F. BRASS et al., "Structure and Function of the Human Platelet Thrombin Receptor. Studies Using Monoclonal Antibodies Directed Against a Defined Domain within the Receptor N Terminus", pages 13795-13798. *
SCIENCE, Volume 222, issued 09 December 1983, K.Y. HUI et al., "Monoclonal Antibodies to a Synthetic Fibrin-Like Peptide Bind to Human Fibrin But Not Fibrinogen", pages 1129-1132. *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996010749A1 (fr) * 1994-09-29 1996-04-11 Mount Sinai School Of Medicine Of The City University Of New York Test d'evaluation du blocage des plaquettes sanguines
US5763199A (en) * 1994-09-29 1998-06-09 Mount Sinai School Of Medicine Of The City University Of New York Platelet blockade assay
US5854005A (en) * 1994-09-29 1998-12-29 Mount Sinai School Of Medicine Platelet blockade assay
US8574828B2 (en) 2003-07-08 2013-11-05 Accumetrics, Inc. Controlled platelet activation to monitor therapy of ADP antagonists
US9341637B2 (en) 2003-07-08 2016-05-17 Accumetrics Inc. Controlled platelet activation to monitor therapy of ADP antagonists
US9506938B2 (en) 2003-07-08 2016-11-29 Accumetrics, Inc. Methods for measuring platelet reactivity of individuals treated with drug eluting stents
WO2009098689A1 (fr) * 2008-02-07 2009-08-13 Hadasit Medical Research Services & Development Limited Immunodétection d'un état cancéreux chez un sujet
CN101999077A (zh) * 2008-02-07 2011-03-30 哈达斯特医学服务与开发有限公司 受试者中癌状态的免疫检测
CN107102134A (zh) * 2017-03-02 2017-08-29 江苏华冠生物技术股份有限公司 一种适用于末梢血检测用的样品稀释液

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