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WO1994012871B1 - Analyse utilisant la focalisation isoelectrique - Google Patents

Analyse utilisant la focalisation isoelectrique

Info

Publication number
WO1994012871B1
WO1994012871B1 PCT/US1993/011467 US9311467W WO9412871B1 WO 1994012871 B1 WO1994012871 B1 WO 1994012871B1 US 9311467 W US9311467 W US 9311467W WO 9412871 B1 WO9412871 B1 WO 9412871B1
Authority
WO
WIPO (PCT)
Prior art keywords
gradient
analyte
complex
detecting
binding moiety
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1993/011467
Other languages
English (en)
Other versions
WO1994012871A1 (fr
Filing date
Publication date
Priority claimed from US07/981,814 external-priority patent/US5376249A/en
Application filed filed Critical
Priority to JP51338894A priority Critical patent/JP3397243B2/ja
Priority to DE69314969T priority patent/DE69314969T2/de
Priority to AU56790/94A priority patent/AU5679094A/en
Priority to EP94902406A priority patent/EP0670999B1/fr
Publication of WO1994012871A1 publication Critical patent/WO1994012871A1/fr
Publication of WO1994012871B1 publication Critical patent/WO1994012871B1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Abstract

L'invention se rapporte à des procédés et à un appareil déterminant la présence d'un ou plusieurs analytes dans un échantillon. L'on détecte la présence d'un complexe d'un analyte et d'une fraction de liaison spécifique à l'analyte en un point du gradient étendu du pH correspondant à un point isoélectrique prédéterminé du complexe dans le gradient. Un champ électrique appliqué au gradient étendu du pH avant la détection du complexe transporte le complexe vers le point du gradient du pH correspondant au point isoélectrique prédéterminé. La fraction de liaison spécifique à l'analyte est de préférence pourvue d'un marqueur détectable, tel qu'un marqueur fluorescent. L'on peut déterminer un paramètre, par ex., une intensité de fluorescence, indiquant la quantité du complexe en un point du gradient du pH correspondant au point isoélectrique prédéterminé afin de quantifier un analyte.
PCT/US1993/011467 1992-11-25 1993-11-23 Analyse utilisant la focalisation isoelectrique Ceased WO1994012871A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP51338894A JP3397243B2 (ja) 1992-11-25 1993-11-23 等電点電気泳動法を用いた分析
DE69314969T DE69314969T2 (de) 1992-11-25 1993-11-23 Analyse durch isoelektrische fokussierung
AU56790/94A AU5679094A (en) 1992-11-25 1993-11-23 Analysis utilizing isoelectric focusing
EP94902406A EP0670999B1 (fr) 1992-11-25 1993-11-23 Analyse utilisant la focalisation isoelectrique

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/981,814 US5376249A (en) 1992-11-25 1992-11-25 Analysis utilizing isoelectric focusing
US981,814 1992-11-25

Publications (2)

Publication Number Publication Date
WO1994012871A1 WO1994012871A1 (fr) 1994-06-09
WO1994012871B1 true WO1994012871B1 (fr) 1994-07-21

Family

ID=25528672

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1993/011467 Ceased WO1994012871A1 (fr) 1992-11-25 1993-11-23 Analyse utilisant la focalisation isoelectrique

Country Status (7)

Country Link
US (1) US5376249A (fr)
EP (1) EP0670999B1 (fr)
JP (1) JP3397243B2 (fr)
AU (1) AU5679094A (fr)
CA (1) CA2148821A1 (fr)
DE (1) DE69314969T2 (fr)
WO (1) WO1994012871A1 (fr)

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