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WO1994000584A3 - Artemisia annua transformee avec des rhizogenes d'agrobacterium - Google Patents

Artemisia annua transformee avec des rhizogenes d'agrobacterium Download PDF

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Publication number
WO1994000584A3
WO1994000584A3 PCT/US1993/005950 US9305950W WO9400584A3 WO 1994000584 A3 WO1994000584 A3 WO 1994000584A3 US 9305950 W US9305950 W US 9305950W WO 9400584 A3 WO9400584 A3 WO 9400584A3
Authority
WO
WIPO (PCT)
Prior art keywords
annua
transformed
transformation
artemisia annua
agrobacterium rhizogenes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1993/005950
Other languages
English (en)
Other versions
WO1994000584A2 (fr
Inventor
Pamela J Weathers
Ronald D Cheetham
Alexander Diiorio
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Worcester Polytechnic Institute
Original Assignee
Worcester Polytechnic Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Worcester Polytechnic Institute filed Critical Worcester Polytechnic Institute
Publication of WO1994000584A2 publication Critical patent/WO1994000584A2/fr
Publication of WO1994000584A3 publication Critical patent/WO1994000584A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
    • C07D493/12—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains three hetero rings
    • C07D493/20—Spiro-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/181—Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Procédé de transformation d'Artemisia annua avec des rhizogènes d'Agrobactérium. Plus particulièrement cette invention concerne un procédé de production d'une culture cellulaire de racines poilues de rhizogènes d'Agrobactérium. Le procédé de transformation de A. annua se caractérise par la transformation de plantes entières avec des rhizogènes d'Agrobactérium et par la transformation de cultures de racines de A. annua avec des rhizogènes d'Agrobactérium. Cette invention concerne également la plante A. annua transformée ainsi qu'un procédé d'extraction de quantités accrues d'un métabolite secondaire de plante issu des tissus de la plante entraînant une perte limitée de la viabilité des tissus étant donné qu'on rend perméable de manière réversible la membrane des tissus.
PCT/US1993/005950 1992-06-23 1993-06-22 Artemisia annua transformee avec des rhizogenes d'agrobacterium Ceased WO1994000584A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US90291692A 1992-06-23 1992-06-23
US07/902,916 1992-06-23

Publications (2)

Publication Number Publication Date
WO1994000584A2 WO1994000584A2 (fr) 1994-01-06
WO1994000584A3 true WO1994000584A3 (fr) 1994-03-31

Family

ID=25416616

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1993/005950 Ceased WO1994000584A2 (fr) 1992-06-23 1993-06-22 Artemisia annua transformee avec des rhizogenes d'agrobacterium

Country Status (1)

Country Link
WO (1) WO1994000584A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9606579D0 (en) * 1996-03-28 1996-06-05 Phytotech Ltd Pharmaceutical composition and methods for the manufacture thereof
EP0982404A1 (fr) * 1998-08-27 2000-03-01 Wallaart, Thorvald Eelco ADN codant pour amorpha-4,11-diène synthase
CN102127566B (zh) * 2010-12-17 2012-04-18 湖南农业大学 黄花蒿的遗传转化方法
CN104651373B (zh) * 2015-02-03 2018-02-09 中国人民解放军第二军医大学 青蒿AaGTD1基因及其编码的蛋白与应用
US11299700B1 (en) 2021-02-19 2022-04-12 Acequia Biotechnology, Llc Bioreactor containers and methods of growing hairy roots using the same
CN113832178A (zh) * 2021-09-08 2021-12-24 浙江省农业科学院 一种发根农杆菌介导的菜用豌豆遗传转化体系建立的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4952603A (en) * 1988-06-20 1990-08-28 Elferaly Farouk S Method for the isolation of artemisinin from Artemisia annua

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4952603A (en) * 1988-06-20 1990-08-28 Elferaly Farouk S Method for the isolation of artemisinin from Artemisia annua

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DIIORIO, A.A., ET AL.: "Modelling of hairy root growth", ABSTRACTS OF PAPERS OF AMERICAN CHEMICAL SOCIETY, vol. 205, no. 1-2, 1993, pages BIOT152 *
FLORES, H.E., ET AL.: "Production of polyacetylenes and thiopenes in heterotrophic and photosynthetic root cultures of Asteraceae", CHEMISTRY AND BIOLOGY OF NATURALLY OCCURRING ACETYLENES AND RELATED COMPOUNDS, 1988, EDITED BY J. LAM, ET AL., ELSEVIER AMSTREDAM., pages 233 - 254 *
HAMILL, J.D., ET AL.: "New routes to plant secondary products", BIOTECHNOLOGY, vol. 5, 1987, NEW YORK US, pages 800 - 804, XP002008146, DOI: doi:10.1038/nbt0887-800 *
NAIR, M.S.R., ET AL.: "Production of artemisinin in tissue cultures of Aretemisia annua", JOURNAL OF NATURAL PRODUCTS, vol. 49, no. 3, 1986, pages 504 - 507, XP001344656 *
PARR, A.J.: "The production of secondary metabolites by plant cell cultures", JOURNAL OF BIOTECHNOLOGY, vol. 10, 1989, AMSTERDAM NL, pages 1 - 26, XP023698791, DOI: doi:10.1016/0168-1656(89)90089-8 *
SIGNS, M.W., ET AL.: "The biosynthetic potential of plant roots", BIOESSAYS, vol. 12, no. 1, January 1990 (1990-01-01), pages 7 - 13 *

Also Published As

Publication number Publication date
WO1994000584A2 (fr) 1994-01-06

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