[go: up one dir, main page]

WO1993013797A3 - Detoxified lps-cholera toxin conjugate vaccine for prevention of cholera - Google Patents

Detoxified lps-cholera toxin conjugate vaccine for prevention of cholera Download PDF

Info

Publication number
WO1993013797A3
WO1993013797A3 PCT/US1993/000253 US9300253W WO9313797A3 WO 1993013797 A3 WO1993013797 A3 WO 1993013797A3 US 9300253 W US9300253 W US 9300253W WO 9313797 A3 WO9313797 A3 WO 9313797A3
Authority
WO
WIPO (PCT)
Prior art keywords
cholera
lps
conjugates
cholera toxin
disclosed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1993/000253
Other languages
French (fr)
Other versions
WO1993013797B1 (en
WO1993013797A2 (en
Inventor
Shousun C Szu
John B Robbins
Rajesh K Gupta
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Health and Human Services
Government of the United States of America
Original Assignee
US Department of Health and Human Services
Government of the United States of America
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by US Department of Health and Human Services, Government of the United States of America filed Critical US Department of Health and Human Services
Priority to EP93903428A priority Critical patent/EP0623026A1/en
Priority to JP5512624A priority patent/JPH07503238A/en
Priority to AU34696/93A priority patent/AU678549B2/en
Publication of WO1993013797A2 publication Critical patent/WO1993013797A2/en
Publication of WO1993013797A3 publication Critical patent/WO1993013797A3/en
Publication of WO1993013797B1 publication Critical patent/WO1993013797B1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/107—Vibrio
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/61—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule the organic macromolecular compound being a polysaccharide or a derivative thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/60—Medicinal preparations containing antigens or antibodies characteristics by the carrier linked to the antigen
    • A61K2039/6087—Polysaccharides; Lipopolysaccharides [LPS]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Communicable Diseases (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

A vaccine formulation comprising conjugates of detoxified LPS with proteins including cholera toxin (CT) is disclosed. Treatment with hydrazine (DeA-LPS) reduces the endotoxic properties of the LPS to clinically acceptable levels and results in a larger and more antigenic molecule than the saccharide produced by acid hydrolysis. The conjugates utilizing the cholera toxin of V. cholerae are disclosed which have low levels of pyrogen, no toxic activity upon Chinese hamster overay cells and elicit booster responses of vibriocidal and CT antibodies when injected subcutaneously as saline solutions into mice. The conjugates produced as a cholera vaccine induce the same antibodies as parenterally injected cellular vaccines but have improved safety and immunologic properties.
PCT/US1993/000253 1992-01-16 1993-01-14 Detoxified lps-cholera toxin conjugate vaccine for prevention of cholera Ceased WO1993013797A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP93903428A EP0623026A1 (en) 1992-01-16 1993-01-14 Detoxified lps-cholera toxin conjugate vaccine for prevention of cholera
JP5512624A JPH07503238A (en) 1992-01-16 1993-01-14 Detoxified LPS-cholera toxin conjugate vaccine for cholera prevention
AU34696/93A AU678549B2 (en) 1992-01-16 1993-01-14 Detoxified LPS-cholera toxin conjugate vaccine for prevention of cholera

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82145392A 1992-01-16 1992-01-16
US07/821,453 1992-01-16

Publications (3)

Publication Number Publication Date
WO1993013797A2 WO1993013797A2 (en) 1993-07-22
WO1993013797A3 true WO1993013797A3 (en) 1993-10-28
WO1993013797B1 WO1993013797B1 (en) 1994-01-20

Family

ID=25233450

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1993/000253 Ceased WO1993013797A2 (en) 1992-01-16 1993-01-14 Detoxified lps-cholera toxin conjugate vaccine for prevention of cholera

Country Status (5)

Country Link
EP (1) EP0623026A1 (en)
JP (1) JPH07503238A (en)
AU (1) AU678549B2 (en)
CA (1) CA2128212A1 (en)
WO (1) WO1993013797A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1420897A (en) * 1996-12-18 1998-07-15 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Conjugate vaccine for (salmonella paratyphi) a
US6531131B1 (en) 1999-08-10 2003-03-11 The United States Of America As Represented By The Department Of Health And Human Services Conjugate vaccine for Neisseria meningitidis
US7749511B2 (en) 2000-04-18 2010-07-06 Endobiologics, Incorporated Anti-sepsis conjugate vaccine
EP1278548A2 (en) 2000-04-18 2003-01-29 Endobiologics, Incorporated Lipopolysaccharide-conjugate vaccine for sepsis treatment
US7527797B1 (en) 2000-09-01 2009-05-05 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Vibrio cholerae 0139 conjugate vaccines
WO2002080964A1 (en) * 2001-04-06 2002-10-17 Institut Pasteur Conjugate vaccine composed of the polysaccharide moiety of the lipopolysaccharide of vibrio cholerae 0139 bound to tetanus toxoid
AU2002309259A1 (en) * 2002-05-09 2003-11-11 Massimo Porro Improved polysaccharide and glycoconjugate vaccines_____________
BR0316271A (en) * 2002-11-14 2005-10-11 Inst Finlay Ct De Investigacio Method for obtaining multivalent conjugate vaccine preparations, multivalent vaccine composition and use thereof
US8048432B2 (en) 2003-08-06 2011-11-01 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Polysaccharide-protein conjugate vaccines
CN102824632A (en) * 2012-09-12 2012-12-19 北京民海生物科技有限公司 Polysaccharide conjugate vaccine of vibrio cholera group O1, preparation method and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2532850A1 (en) * 1982-09-15 1984-03-16 Pasteur Institut Immunogenic conjugates between a hapten and a carrier molecule derived from a toxin, vaccines comprising them and method for obtaining them
EP0306607A1 (en) * 1983-03-14 1989-03-15 Miles Inc. Vaccines for gram-negative bacteria

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2532850A1 (en) * 1982-09-15 1984-03-16 Pasteur Institut Immunogenic conjugates between a hapten and a carrier molecule derived from a toxin, vaccines comprising them and method for obtaining them
EP0306607A1 (en) * 1983-03-14 1989-03-15 Miles Inc. Vaccines for gram-negative bacteria

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
BROWN, F., R. M. CHANOCK AND R. A. LERNER (ED.). NEW APPROACHES TO IMMUNIZATION: DEVELOPING VACCINES AGAINST PARASITIC, BACTERIAL, AND VIRAL DISEASES; 1986, pages 231 - 234 KABIR, S. 'PREPARATION AND IMMUNOGENICITY OF A SEMISYNTHETIC BIVALENT CELL-SURFACE PROTEIN-POLYSACCHARIDE CONJUGATE OF VIBRIO-CHOLERAE.' *
EUR. J. BIOCHEM., VOL. 97, NO. 2, PAGE(S) 623-9, July 1979, TANAMOTO K ET AL. 'Regions of the lipopolysaccharide of Pseudomonas aeruginosa essential for antitumor and interferon-inducing activities.' *
INFECT. IMMUN., VOL. 13, NO. 3, PAGE(S) 735-740, 1976, WASHINGTON, US SVENNERHOLM A M ET AL. 'Synergistic protective effect in rabbits of immunization with vibrio cholerae lipopolysaccharide and toxin/toxoid.' *
INFECT. IMMUN., VOL. 60, NO. 8, PAGE(S) 3201-3208 1992, GUPTA R K ET AL 'Synthesis, characterization, and some immunological properties of conjugates composed of the detoxified lipopolysaccharide of Vibrio cholerae O1 serotype inaba bound to cholera toxin.' *
J. INFECT. DIS., VOL. 161, NO. , PAGE(S) 821-832, 1990, J. B. ROBBINS & R. SCHNEERSON 'Polysaccharide-protein conjugates: a new generation of vaccines.' *
MICROBIOL. ABSTR. B vol. 6, no. 1, October 1970, page 72 M. TORII ET AL. 'Immunochemical studies on O-antigens of Vibrio parahaemolyticus. 2. Effect of alkali and acid on specificity.' *
MICROBIOL. IMMUNOL., VOL. 23, NO. 2, PAGE(S) 87-94, 1979, EGAWA K ET AL. 'Endotoxic glycolipid as a potent depressor of the hepatic drug-metabolizing enzyme systems in mice.' *
PATENT ABSTRACTS OF JAPAN vol. 010, no. 287 (C-375)30 September 1986 *
SOUTHEAST ASIAN J TROP MED PUBLIC HEALTH., VOL. 18, NO. 2, PAGE(S) 142-148, 1987, CHAICUMPA W ET AL. 'ORAL VACCINE AGAINST CHOLERA PREPARED FROM VIBRIO-CHOLERAE ANTIGENS.' *
VACCINE, VOL. 9, NO. 1, PAGE(S) 53-59, January 1991, GB M. CHONGSA-NGUAN ET AL. 'IMMUNOGENECITY OF TWO FORMULATIONS OF ORAL CHOLERA VACCINES IN THAI VOLUNTEERS' *

Also Published As

Publication number Publication date
AU678549B2 (en) 1997-06-05
AU3469693A (en) 1993-08-03
WO1993013797A2 (en) 1993-07-22
CA2128212A1 (en) 1993-07-22
JPH07503238A (en) 1995-04-06
EP0623026A1 (en) 1994-11-09

Similar Documents

Publication Publication Date Title
AU676497B2 (en) Pertussis toxin used as a carrier protein with non-charged saccharides in conjugate vaccines
EP0118831B1 (en) Vaccines for gram-negative bacteria and method for the preparation thereof
US20120014991A1 (en) Novel, non-antigenic, mucosal adjuvant formulation which modulates the effects of substances, including vaccine antigens, in contact with mucosal body surfaces
EP1238677A3 (en) Parenteral fat emulsions comprising edetate as preservative
BR9507400A (en) Immunogenic compositions of group a streptococal polysaccharide and processes
WO1993013797A3 (en) Detoxified lps-cholera toxin conjugate vaccine for prevention of cholera
CA2208047A1 (en) Increasing creatine and glycogen concentration in muscle
Hikino et al. Isolation and hypoglycemic activity of trichosans A, B, C, D, and E: glycans of Trichosanthes kirilowii roots1
CA2158651C (en) Multicomponent clostridial vaccines using saponin adjuvants
Northrup et al. Response of monkeys to immunization with cholera toxoid, toxin, and vaccine: reversion of cholera toxoid
WO1993013797B1 (en) Detoxified lps-cholera toxin conjugate vaccine for prevention of cholera
CN100526876C (en) Unmethylated CpG dinucleotide content detection method
CA2255867C (en) Chitosan induced immunopotentiation
CA2170142A1 (en) Composition and method for stimulation of reproductive performance
Stone et al. Tick‐paralysis toxoid: an effective immunizing agent against the toxin of Ixodes holocyclus
Wachter et al. Enhancement of the immunogenicity of phase I antigen of Coxiella burnetii
Brown et al. Morphological, chemical and antigenic characterization of M-1 N-acetylmuramidase-digested cell walls of the oral pathogenic bacterium Actinomyces viscosus T14V and T14AV
KIM et al. Effects of Scopolamine Patches on Pupil and Accommodation
Zhuang Preliminary study of anti-E. coli immune RNA induced cell-mediated immunity by leukocyte adherence inhibition test.
Seifert et al. The effect of muramyl dipeptide on the utilization of pyrinidine precursors for DNA synthesis in rat
Riveau et al. Possibility of a direct pyrogenic effect of muramyl dipeptide
Resl et al. Development of local morphological changes after intradermal inoculation of Q-fever chemovaccine
NZ241920A (en) Vaccine preparations containing vitamin b12 (cyanocobalamin)

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU CA JP

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
COP Corrected version of pamphlet

Free format text: PAGES 1/4-4/4,DRAWINGS,REPLACED BY NEW PAGES 1/5-5/5;DUE TO LATE TRANSMITTAL BY THE RECEIVING OFFICE

EX32 Extension under rule 32 effected after completion of technical preparation for international publication
AK Designated states

Kind code of ref document: A3

Designated state(s): AU CA JP

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LU MC NL PT SE

WWE Wipo information: entry into national phase

Ref document number: 2128212

Country of ref document: CA

Ref document number: 1993903428

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1993903428

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1993903428

Country of ref document: EP