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WO1992021693A1 - Marqueurs d'adn polymorphiques contenant trois sequences repetitives microsatellites a haute capacite d'information - Google Patents

Marqueurs d'adn polymorphiques contenant trois sequences repetitives microsatellites a haute capacite d'information Download PDF

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Publication number
WO1992021693A1
WO1992021693A1 PCT/US1992/004195 US9204195W WO9221693A1 WO 1992021693 A1 WO1992021693 A1 WO 1992021693A1 US 9204195 W US9204195 W US 9204195W WO 9221693 A1 WO9221693 A1 WO 9221693A1
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WIPO (PCT)
Prior art keywords
seq
set forth
sequence
nucleotide sequence
nucleotide
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PCT/US1992/004195
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English (en)
Inventor
Michael H. Polymeropoulos
Carl R. Merrill
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US Department of Health and Human Services
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US Department of Health and Human Services
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Application filed by US Department of Health and Human Services filed Critical US Department of Health and Human Services
Priority to CA002110059A priority Critical patent/CA2110059C/fr
Priority to JP5500444A priority patent/JPH06509222A/ja
Priority to DE69233657T priority patent/DE69233657T2/de
Priority to AU21569/92A priority patent/AU663509B2/en
Priority to EP92912797A priority patent/EP0663923B1/fr
Publication of WO1992021693A1 publication Critical patent/WO1992021693A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Definitions

  • This application relates to genetic testing with polymorphic DNA markers having repeat sequences to provide a rapid and convenient high resolution process for distinguishing target nucleic acid segments on the basis of nucleotide differences according to human individualization wherein the nucleic acid segments differ in size.
  • Restriction fragment length polymorphism mappings are based on changes at the restriction site. They are accurate but not very informative (PIC [ 0.3).
  • PIC perceptual C
  • the major problem with RFLPs is the inability of a test to detect changes that do not affect cleavage with a restriction endonuclease. As in many of the test methods in the DNA art, the methods used to detect RFLPs are very labor intensive and expensive, especially the techniques which includes Southern blot analysis.
  • Another technique for detecting specific mutations in particular DNA segment involves hybridizing DNA segments which are being analyzed (target DNA) with a complimentary, labeled oligonucleotide probe.
  • target DNA DNA segments which are being analyzed
  • a complimentary, labeled oligonucleotide probe See Nucl. Acids Res. 9., 879-894 (1981). Since DNA duplexes containing even a single base pair mismatch exhibit high thermal instability, the differential melting temperature can be used to distinguish target DNAs that are perfectly complimentary to the probe from target DNAs that only differ by a single nucleotide.
  • This method has been adapted to detect the presence or absence of a specific restriction site, U.S. Patent No. 4,683,194. The method involves using an end-labeled oligonucleotide probe spanning a restriction site which is hybridized to a target DNA.
  • the hybridized duplex of DNA is then incubated with the restriction enzyme appropriate for that site.
  • Reformed restriction sites will be cleaved by digestion in the pair of duplexes between the probe and target by using the restriction endonuclease.
  • the specific restriction site is present in the target DNA if shortened probe molecules are detected.
  • primer extension process which consists of hybridizing a labeled oligonucleotide primer to a template RNA or DNA and then using a DNA polymerase and deoxynucleoside triphosphates to extend the primer to the 5' end of the template. Resolution of the labeled primer extension product is then done by fractionating on the basis of size, e.g. , by electrophoresis via a denaturing polyacry1amide gel. This process is often used to compare homologous DNA segments and to detect differences due to nucleotide insertion or deletion. Differences due to nucleotide substitution are not detected since size is the sole criterion used to characterize the primer extension product.
  • nucleotide analogs can be used to identify changes since they can cause an electro- phoretic mobility shift. See, U.S. Patent 4,879,214.
  • VNTR's or Jeffrey's probes which the FBI is using to test and identify DNA chains
  • VNTR's or Jeffrey's probes are very informative but labor intensive, in distinction to microsatellites as our which are equally informative PCR based polymormismic.
  • An object of the present invention is to provide a fast and accurate test for measuring the subtle differences in individuals by way of genetic testing.
  • Another object of the invention relates to polymorphic markers that can be used for human individualization.
  • a further object of the invention is to provide a fast and accurate technique for measuring the subtle differences in individuals by way of genetic testing that can be applied in multiple areas, e.g. , forensic screening, paternity and prenatal screening and genetic mapping.
  • a still further object is to provide an improved method for conducting a PCR procedure using an effective amount of a nucleotide according to the present invention and to provide an PCR assay kit comprising • an effective amount of a nucleotide according to the present invention and ancillary PCR reagents.
  • Figure 1 relates to a nucleotide sequence according to SEQ ID N0:1.
  • Figure 2 relates to a nucleotide sequence according to SEQ ID NO:2.
  • Figure 3 relates to a nucleotide sequence according to SEQ ID NO:3.
  • Figure 4 relates to a nucleotide sequence according to SEQ ID NO:4.
  • Figure 5 relates to a nucleotide sequence according to SEQ ID NO:5.
  • Figure 6 relates to a nucleotide sequence according to SEQ ID NO:6.
  • Figure 7 relates to a nucleotide sequence according to SEQ ID NO:7.
  • Figure 8 relates to a nucleotide sequence according to SEQ ID NO:8.
  • Figure 9 relates to a nucleotide sequence according to SEQ ID NO:9.
  • Figure 10 relates to a nucleotide sequence according to SEQ ID NO:10.
  • Figure 11 relates to a nucleotide sequence according to SEQ ID NO:
  • Figure 12 relates to a nucleotide sequence according to SEQ ID NO:12.
  • Figure 13 relates to a nucleotide sequence according to SEQ ID NO:13.
  • Figure 14 relates to a nucleotide sequence according to SEQ ID NO:14.
  • Figure 15 relates to a nucleotide sequence according to SEQ ID NO:15.
  • Figure 16 relates to a nucleotide sequence according to SEQ ID NO:16.
  • Figure 17 relates to a nucleotide sequence according to SEQ ID NO:17.
  • Figure 18 relates to a nucleotide sequence according to SEQ ID NO:18.
  • Figure 19 relates to a nucleotide sequence according to SEQ ID NO:19.
  • Figure 20 relates to a nucleotide sequence according to SEQ ID NO:20.
  • Figure 21 relates to a nucleotide sequence according to SEQ ID NO:21.
  • Figure 22 relates to a nucleotide sequence according to SEQ ID NO:22.
  • Figure 23 relates to a nucleotide sequence according to SEQ ID NO:23.
  • Figure 24 relates to a nucleotide sequence according to SEQ ID NO:24.
  • Figure 25 relates to a nucleotide sequence according to SEQ ID NO:25.
  • Figure 26 relates to a nucleotide sequence according to SEQ ID NO:26.
  • Figure 27 relates to a nucleotide sequence according to SEQ ID NO:27.
  • Figure 28 relates to a nucleotide sequence according to SEQ ID NO:28.
  • Figure 29 relates to a nucleotide sequence according to SEQ ID NO:29.
  • Figure 30 relates to a nucleotide sequence according to SEQ ID NO:30.
  • Figure 31 relates to a nucleotide sequence according to SEQ ID NO:31.
  • Figure 32 relates to a nucleotide sequence according to SEQ ID NO:32.
  • Figure 33 relates to a nucleotide sequence according to SEQ ID NO:33.
  • Figure 34 relates to a nucleotide sequence according to SEQ ID NO:34.
  • Figure 35 relates to a nucleotide sequence according to SEQ ID NO:35.
  • Figure 36 relates to a nucleotide sequence according to SEQ ID NO:36.
  • Figure 37 relates to a nucleotide sequence according to SEQ ID NO:37.
  • Figure 38 relates to a nucleotide sequence according to SEQ ID NO:38.
  • Figure 39 relates to a nucleotide sequence according to SEQ ID NO:39.
  • Figure 40 relates to a nucleotide sequence according to SEQ ID NO:40.
  • Figure 41 relates to a nucleotide sequence according to SEQ ID NO:41.
  • Figure 42 relates to a nucleotide sequence according to SEQ ID NO:42.
  • Figure 43 relates to a nucleotide sequence according to SEQ ID NO:43.
  • Figure 44 relates to a nucleotide sequence according to SEQ ID NO:44.
  • Figure 45 relates to a nucleotide sequence according to SEQ ID NO:45.
  • Figure 46 relates to a nucleotide sequence according to SEQ ID NO: 6.
  • Figure 47 relates to a nucleotide sequence according to SEQ ID NO:47.
  • Figure 48 relates to a nucleotide sequence according to SEQ ID NO:48.
  • Figure 49 relates to a nucleotide sequence according to SEQ ID NO:49.
  • Figure 50 relates to a nucleotide sequence according to SEQ ID NO:50.
  • Figure 51 relates to a nucleotide sequence according to SEQ ID NO:51.
  • Figure 52 relates to a nucleotide sequence according to SEQ ID NO:52.
  • Figure 53 relates to a nucleotide sequence according to SEQ ID NO:53.
  • Figure 54 relates to a nucleotide sequence according to SEQ ID NO:54.
  • Figure 55 relates to a nucleotide sequence according to SEQ ID NO:55.
  • Figure 56 relates to a nucleotide sequence according to SEQ ID NO:56.
  • Figure 57 relates to a nucleotide sequence according to SEQ ID NO:57.
  • Figure 58 relates to a nucleotide sequence according to SEQ ID NO:58.
  • Figure 59 relates to a nucleotide sequence according to SEQ ID NO:59.
  • Figure 60 relates to a nucleotide sequence according to SEQ ID NO:60.
  • Figure 61 relates to a nucleotide sequence according to SEQ ID NO:61.
  • Figure 62 relates to a nucleotide sequence according to SEQ ID NO:62.
  • Figure 63 relates to a nucleotide sequence according to SEQ ID NO:63.
  • the present invention provides a fast and accurate test for measuring subtle genetic differences in individuals by way of genetic testing.
  • the invention further relates to polymorphic markers (two tetranucleotide and one dinucleotide repeat polymorphisms) that can be used for human individualization.
  • the invention further relates to 27 other polymorphic markers useful for human individualization.
  • Applications for the technique and markers according to the invention are for example, in forensic screening, in paternity and prenatal screening as well as in genetic mapping.
  • the invention relates to polymorphic markers (two tetranucleotide, one dinucleotide repeat polymorphisms and 27 other unique polymorphic markers) that are useful for human individualization of forensic screen, and for paternity and prenatal screening as well as genetic mapping.
  • the markers according to the present invention have high polymorphism information content (PIC) values.
  • PIC polymorphism information content
  • the first three markers are characterized by sets of oligonucleotide primers as follows:
  • polymorphic markers two tetranucleotide and one dinucleotide repeat polymorphisms which are also accompanied by beginning and ending nucleotide sequences .
  • the amplified DNA segment can then be resolved by electrophoresis and autoradiography.
  • a resulting autoradiography can then be analyzed for its similarity to another DNA segment autoradiography.
  • electrophoretic motility enhancing DNA analogs may optionally be used to increase the accuracy of the electrophoresis step.
  • Twenty-seven other primary pair sequences for detecting unique polymorphisms are sequences according to SEQ ID NO:10 through SEQ ID NO:63.
  • the described polymorphisms are useful for human sample individualization, because of their high PIC values. Since the described polymorphisms are based on the polymerase chain reaction, only minute amounts of genomic DNA are required for each test. The target sequences range from 69-260 bps in length so that high molecular weight DNA is not necessary and common problems such as shearing of DNA will have minimal impact on the performance of the assay. The assay is easy to perform and results can be obtained within 24 hours. Microsatellite repeat polymorphisms have been shown to be useful tools in DNA analysis. The 27 polymorphisms described here are original and are based on previously sequenced human genes. The most commonly used technique in forensic screening is based on minisatellite markers in distinction to the PCR able icrosatellites described here.
  • the 27 markers are characterized by sets of oligonucleotide primers as follows:
  • the invention relates to a method for conducting a PCR procedure comprising using an effective amount of at least one nucleotide according to according to the invention as set forth above, wherein the nucleotide is part of a primer pair of nucleotides selected from the group of nucleotide pairs consisting of a) a nucleotide sequence having the sequence as set forth in SEQ ID N0:1 and a nucleotide sequence as set forth in SEQ ID NO:2; b) a nucleotide sequence having the sequence as set forth in SEQ ID NO:3 and a nucleotide sequence as set forth in SEQ ID NO:4; c) a nucleotide sequence having the sequence as set forth in SEQ ID NO:5 and a nucleotide sequence as set forth in SEQ ID NO:6; d) a nucleotide sequence having the sequence as set forth in SEQ ID NO:10 and a nucleotide sequence as set forth in SEQ ID NO:11;
  • the invention further relates to an assay for measuring the subtle differences in genetic material regarding an added or omitted set of dinucleotide or tetranucleotide repeat polymorphisms selected from the group consisting of a sequence according to SEQ ID NO:7, a sequence according to SEQ ID NO:8 and a sequence according to SEQ ID NO:9, which comprises a. obtaining nucleotide segments comprising said repeat polymorphisms in an amount effective for testing, b. amplifying said segments by a PCR procedure using a pair of oligonucleotide primers capable of amplifying said polymorphism containing segments, c. resolving the amplified segments using page gels electrophoresis, and d. comparing the resolved segments by autoradiography to observe the differences in migration patterns due to length variation.
  • the invention further relates to an assay for measuring the subtle differences in genetic material regarding an added or omitted set of dinucleotide or tetranucleotide repeat polymorphisms selected from the group consisting of a sequence according to SEQ ID NO:7, a sequence according to SEQ ID NO:8 and a sequence according to SEQ ID NO:9, which comprises a. obtaining nucleotide segments comprising said repeat polymorphisms in an amount effective for testing, b.
  • oligonucleotide primers selected from the group consisting of a sequence according to SEQ ID NO:l, a sequence according to SEQ ID NO:2, a sequence according to SEQ ID NO:3, a sequence according to SEQ ID NO:4, a sequence according to SEQ ID NO:5, or a sequence according to SEQ ID NO:6, c. resolving the amplified segments using page gels electrophoresis, and d. comparing the resolved segments by autoradiography to observe the differences in migration patterns due to length variation.
  • the invention relates to an assay kit for conducting a PCR procedure comprising an effective amount of at least one nucleotide having a sequence according to the invention as set forth above, wherein the nucleotide is part of a primer pair of nucleotides selected from the group of nucleotide pairs consisting of a) a nucleotide sequence having the sequence as set forth in SEQ ID N0:1 and a nucleotide sequence as set forth in SEQ ID NO:2; b) a nucleotide sequence having the sequence as set forth in SEQ ID NO:3 and a nucleotide sequence as set forth in SEQ ID NO:4; and c) a nucleotide sequence having the sequence as set forth in SEQ ID NO:5 and a nucleotide sequence as set forth in SEQ ID NO:6, d) a nucleotide sequence having the sequence as set forth in SEQ ID NO:10 and a nucleotide sequence as set forth in SEQ ID NO:
  • the above described polymorphisms are useful for human sample individualization, because of their high PIC values. Since the described polymorphic systems are based on the polymerase chain reaction (PCR), only minute (40 nanograms) amounts of genomic DNA, and genomic DNA sequences.
  • the target sequences range from 92 to 310 base pairs so that high molecular weight DNA is not necessary, and common problems such as shearing of DNA will have minimal impact on the performance of the assay.
  • the assay is easy to perform and results can be obtained within 24 hours. It is not uncommon for results to be available within 3-4 hours.
  • polymorphism corresponding to 1A-27A as described above and characterizes by their 27 primer pairs according to SEQ ID NO:10-SEQ NO:63 are useful for human sample individualization evaluation because of their high PIC values.
  • the assay according to the invention is able to detect very small differences in nucleotide sequences.
  • a single omission or addition of the repeat sequence will change the mobility due to the electrical nature and molecular weight of the target nucleotide sequence. These differences are clearly visible on the autoradiographs after electrophoresis.
  • Microsatellite repeat polymorphisms have been shown to be useful tools in DNA analysis.
  • the three polymorphisms described here are original and are based on previously sequenced genes.
  • the two tetranucleotide repeat markers described can be scored easily since allele sizes differ by four base pairs.
  • the most commonly used technique used in forensic screening is based on minisatellite markers, in distinction to the PCR able microsatellites described in the present invention.
  • the degree of polymorphism in the genetic segments according to the present invention is surprising and unexpected.
  • the high PIC value (approximately 0.9) is totally unexpected. Accordingly, the use of a PCR procedure and PCR primers pairs, such as those primer sequences according to SEQ ID NO:l to SEQ ID NO:6, to detect the polymorphism DNA segment according to the present invention yields excellent results. Further use of primer sequences corresponding to SEQ ID NO:10 through SEQ ID NO:63 to detect the polymorphism yields excellent results. Such results are sufficiently accurate and informative to accurately identify DNA segments and determine degrees of relationship between DNA segments of individuals.
  • the described oligonucleotide primers are used to amplify the target sequences using PCR, under the following conditions:
  • the samples are of DNA are prepared as follows.
  • 60ng of genomic DNA are used as template for PCR with 80ng of each oligonucleotide primer, 0.6 units of Taq Polymerase 50mM KCL, lOmM Tris (pH 8.3), 1.5mM MgCl,, 0.01% gelatin, 200uM of each dGTP, dATP, dTTP, 2.5uM dCTP and 10 icrocuries of alpha P32 dCTP., in a final reaction volume of 15 microliters. The samples are overlaid with 15 microliters of mineral oil to prevent evaporation.
  • PCR is performed in a Techne MW-1 microplate thermocycler under the following conditions denaturation of 94 degrees C for 1.4 min., annealing at 55 degrees C for 2 min., and extension at 72 degrees C for 2 min. The cycle is repeated 30 times with a final extension at 72 degrees C for 10 min. ffxample 3

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Abstract

L'invention concerne des marqueurs polymorphiques (polymorphisme à répétition de deux tétranucléotides et d'un binucléotide, et 27 marqueurs caractérisés par des paires d'amorces 1A-27A) utiles dans l'individualisation humaine. Lesdits marqueurs peuvent être utilisés dans le domaine de la médecine légale, et dans la recherche de paternité et le dépistage prénatal ainsi que dans le mapping génétique. Ces marqueurs sont caractérisés par des ensembles d'amorces d'oligonucléotides selon l'invention utiles dans l'amplification par réaction en chaîne de la polymérase et la résolution des segments d'ADN. L'invention se rapporte également à un dosage servant à mesurer les différences subtiles dans un matériau génétique concernant un ensemble ajouté ou omis de polymorphismes à répétition de binucléotides ou de tétranucléotides. Le procédé de dosage consiste à obtenir une quantité de segments de nucléotide, qui soient efficaces pour réaliser les tests, amplifier les segments par le procédé de réaction en chaîne de la polymérase à l'aide d'au moins une séquence de nucléotides d'amorce selon la présente invention, à résoudre les segments amplifiés par électrophorèse sur gel, et à comparer les segments résolus par autoradiographie afin de déterminer les différences dans les motifs de migration dues aux différences structurales. Le dosage selon l'invention est facile à réaliser et les résultats peuvent être obtenus en 24 heures. Il n'est pas rare que des résultats puissent être obtenus en trois ou quatre heures. L'invention concerne également un procédé amélioré de réaction en chaîne de la polymérase ainsi qu'un kit de dosage par réaction en chaîne de la polymérase comprenant les nucléotides selon la présente invention.
PCT/US1992/004195 1991-05-29 1992-05-27 Marqueurs d'adn polymorphiques contenant trois sequences repetitives microsatellites a haute capacite d'information Ceased WO1992021693A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002110059A CA2110059C (fr) 1991-05-29 1992-05-27 Trois marqueurs polymorphiques a microsequences repetitives d'adn satellite
JP5500444A JPH06509222A (ja) 1991-05-29 1992-05-27 三種の高情報性ミクロサテライト反復多型性ｄｎａマーカ
DE69233657T DE69233657T2 (de) 1991-05-29 1992-05-27 Drei Mikrosatelliten-Repeat-Polymorphe DNA-Marker mit hoher Informationsdichte
AU21569/92A AU663509B2 (en) 1991-05-29 1992-05-27 Three highly informative microsatellite repeat polymorphic DNA markers
EP92912797A EP0663923B1 (fr) 1991-05-29 1992-05-27 Marqueurs d'adn polymorphiques contenant trois sequences repetitives microsatellites a haute capacite d'information

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US70750191A 1991-05-29 1991-05-29
US707,501 1991-05-29
US07/799,828 US5378602A (en) 1991-05-29 1991-11-27 Highly informative microsatellite repeat polymorphic DNA markers twenty-[seven]six
US799,828 1991-11-27

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EP (1) EP0663923B1 (fr)
JP (1) JPH06509222A (fr)
AT (1) ATE339435T1 (fr)
AU (1) AU663509B2 (fr)
DE (1) DE69233657T2 (fr)
ES (1) ES2268682T3 (fr)
WO (1) WO1992021693A1 (fr)

Cited By (5)

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WO1994003640A1 (fr) * 1992-07-31 1994-02-17 Government Of The United States As Represented By Secretary Department Of Health And Human Services Onze marqueurs d'adn polymorphes a repetition de microsatellites extremement instructifs
US5369004A (en) * 1991-05-29 1994-11-29 The United States Of America As Represented By The Department Of Health And Human Services Five highly informative microsatellite repeat polymorphic DNA markers
WO1995017522A3 (fr) * 1993-12-21 1995-11-02 Univ Leicester Identification de sequences repetees simples en tandem
WO1996010648A3 (fr) * 1994-09-30 1997-02-27 Promega Corp Amplification multiplex et loci courts repetes en tandem
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DE69233657T2 (de) 2007-01-11
DE69233657D1 (de) 2006-10-26
JPH06509222A (ja) 1994-10-20
EP0663923A1 (fr) 1995-07-26
AU663509B2 (en) 1995-10-12
US5378602A (en) 1995-01-03
ES2268682T3 (es) 2007-03-16
ATE339435T1 (de) 2006-10-15
EP0663923A4 (fr) 1999-08-04
AU2156992A (en) 1993-01-08
US5369004A (en) 1994-11-29

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