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WO1991015750A1 - Dispositif microfabrique pour le triage de cellules biologiques - Google Patents

Dispositif microfabrique pour le triage de cellules biologiques Download PDF

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Publication number
WO1991015750A1
WO1991015750A1 PCT/GB1991/000542 GB9100542W WO9115750A1 WO 1991015750 A1 WO1991015750 A1 WO 1991015750A1 GB 9100542 W GB9100542 W GB 9100542W WO 9115750 A1 WO9115750 A1 WO 9115750A1
Authority
WO
WIPO (PCT)
Prior art keywords
cells
microfabricated
cell sorting
biological cell
microfabricated device
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB1991/000542
Other languages
English (en)
Inventor
Paul Henry Kaye
Mark Christopher Tracey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CARRI-MED Ltd
Carri Med Ltd
Original Assignee
CARRI-MED Ltd
Carri Med Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CARRI-MED Ltd, Carri Med Ltd filed Critical CARRI-MED Ltd
Publication of WO1991015750A1 publication Critical patent/WO1991015750A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0622—Valves, specific forms thereof distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N11/00—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties

Definitions

  • This invention relates to the sorting of biological cells into spatially separate sub groups.
  • the criteria for sorting may be applied to measurements obtained by any technique or techniques suitable for microfabricated or electronic implementation on a substrate.
  • Microfabrication and microelectronic techniques offer a number of actual and potential measurement techniques that may be applied to characterize biological cells.
  • PCT/GB91/00289 and morphological measurements.
  • morphological measurements When suitably implemented such techniques can be applied on a cell by cell basis.
  • microelectromechani ⁇ al structures and their formation by selective CVD techniques, are disclosed in: "Selective Chemical Vapor Deposition of Tungsten for Mi ⁇ roelectromechani ⁇ al Structures" by N. C. MACDONALD et al. , Sensors and Actuators, 20 (1989) 123-133.
  • the so-called “microtweezers” are activated by control voltages applied to electrodes.
  • such a machine is implemented by utilising a microfabricated moveable structure, preferably microfabricated beams, to direct cells between distinct spatial locations.
  • FIG. 1 is a topologi ⁇ al plan view of a substrate on which a cell sorter is formed.
  • the cells to be sorted whose diameters may be only a few micrometres, are fed sequentially from an entry port 1, via a guiding structure 2, past a generalised sensing device or zone 3.
  • the deflectable beams 4, 5, 6, are set into appropriate states to direct the cell into a particular destination, for example, a hole 7, 8, 9 or 10 etched through the substrate.
  • the destination is structure 9.
  • the beams, along with associated bifurcations can be extended to a tree structure of ' n' levels thereby yielding 2 possible sorting sub ranges.
  • the absolute lengths of the channels are as short as possible while maintaining compatibility with interconnecting structures.
  • the shafts 1, 3, 7-10 may be fabricated by selective etching as disclosed in the copending International Patent Application referred to above; the etched substrate is closed by a glass/silica cover, thereby forming the channels as tunnels.
  • Typical dimensions of the entry port 1 are 500 ⁇ m or less square; of the sensing zone about 100 ⁇ m square; of the channels about 5 to 10 ⁇ m in width and depth; of the holes (shafts) 7 - 10 about 500 ⁇ m or less square; and of the beams, which are typically of constant rectangular section, about 150 ⁇ m long by 3 ⁇ m square.
  • the overall dimensions of the device could be l ⁇ m square.
  • the beams are activated by selective control voltages applied as signals for control circuitry (not shown) operated, for example, under the control of a microprocessor or other computer.
  • the computer is programmed to control the beams' deflection in response to the desired cell-sorting procedural steps.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Fluid Mechanics (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

Appareil de triage de cellules en sous-groupes séparés dans l'espace, comprenant une structure mobile microfabriquée (4, 5, 6) servant à diriger les cellules entre des emplacements spatiaux distincts.
PCT/GB1991/000542 1990-04-09 1991-04-08 Dispositif microfabrique pour le triage de cellules biologiques Ceased WO1991015750A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9008044.1 1990-04-09
GB909008044A GB9008044D0 (en) 1990-04-09 1990-04-09 Microfabricated device for biological cell sorting

Publications (1)

Publication Number Publication Date
WO1991015750A1 true WO1991015750A1 (fr) 1991-10-17

Family

ID=10674166

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1991/000542 Ceased WO1991015750A1 (fr) 1990-04-09 1991-04-08 Dispositif microfabrique pour le triage de cellules biologiques

Country Status (2)

Country Link
GB (1) GB9008044D0 (fr)
WO (1) WO1991015750A1 (fr)

Cited By (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993022054A1 (fr) * 1992-05-01 1993-11-11 Trustees Of The University Of Pennsylvania Analyse fondee sur un resserrement du systeme d'ecoulment
US5304487A (en) * 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
EP0616218A1 (fr) * 1993-03-16 1994-09-21 Hitachi, Ltd. Dispositif micro-réacteur et système d'analyse de très petits échantillons utilisant ce dispositif
US5427946A (en) * 1992-05-01 1995-06-27 Trustees Of The University Of Pennsylvania Mesoscale sperm handling devices
US5486335A (en) * 1992-05-01 1996-01-23 Trustees Of The University Of Pennsylvania Analysis based on flow restriction
US5498392A (en) * 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
DE19520298A1 (de) * 1995-06-02 1996-12-05 Bayer Ag Sortiervorrichtung für biologische Zellen oder Viren
US5587128A (en) * 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5726026A (en) * 1992-05-01 1998-03-10 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US5744366A (en) * 1992-05-01 1998-04-28 Trustees Of The University Of Pennsylvania Mesoscale devices and methods for analysis of motile cells
US5747349A (en) * 1996-03-20 1998-05-05 University Of Washington Fluorescent reporter beads for fluid analysis
WO1998045683A1 (fr) * 1997-04-08 1998-10-15 Smithkline Beecham Plc Dispositif servant a isoler de petites perles polymeres depuis une suspension de ces perles
US5906732A (en) * 1995-11-17 1999-05-25 Yazaki Corporation Apparatus for separating minute substances in liquid
US5950842A (en) * 1995-02-23 1999-09-14 Natec, Reich, Summer Gmbh & Co. Kg Line connector with 90 degree rotation mechanism
US6056860A (en) * 1996-09-18 2000-05-02 Aclara Biosciences, Inc. Surface modified electrophoretic chambers
US6193647B1 (en) 1999-04-08 2001-02-27 The Board Of Trustees Of The University Of Illinois Microfluidic embryo and/or oocyte handling device and method
WO2001019516A1 (fr) * 1999-09-13 2001-03-22 Hoffmann La Roche Systeme de manipulation de suspensions
WO2001049412A1 (fr) * 1999-12-30 2001-07-12 Acreo Ab Procede et dispositif de regulation de debit
WO1999061888A3 (fr) * 1998-05-22 2001-12-13 California Inst Of Techn Trieur de cellules microfabrique
WO2002001189A1 (fr) * 2000-06-26 2002-01-03 Gnothis Holding S.A. Procede de selection de particules
US6432630B1 (en) 1996-09-04 2002-08-13 Scandinanian Micro Biodevices A/S Micro-flow system for particle separation and analysis
WO2003025563A1 (fr) * 2001-09-16 2003-03-27 Chemometec A/S Procede et systeme de detection et eventuellement d'isolement de particules peu abondantes
US6540895B1 (en) * 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
WO2003060486A1 (fr) * 2002-01-10 2003-07-24 Board Of Regents, The University Of Texas System Systeme de tri de flux et procedes associes
WO2003078065A1 (fr) * 2002-03-14 2003-09-25 Micronics, Inc. Dispositif microfluidique a reseau de canaux
US6953676B1 (en) 1992-05-01 2005-10-11 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US7118910B2 (en) 2001-11-30 2006-10-10 Fluidigm Corporation Microfluidic device and methods of using same
EP1567845A4 (fr) * 2002-07-08 2007-07-18 John S Foster Procede et appareil destines a trier des cellules biologiques au moyen d'un dispositif a systeme mecanique microelectrique
WO2007084392A3 (fr) * 2006-01-13 2007-11-08 Micronics Inc Soupapes a actionnement electromagnetique a utiliser dans des structures microfluidiques
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
EP1499453A4 (fr) * 2002-04-17 2009-11-04 Cytonome Inc Procede et appareil de tri de particules
US7691333B2 (en) 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
US7833708B2 (en) 2001-04-06 2010-11-16 California Institute Of Technology Nucleic acid amplification using microfluidic devices
US7867454B2 (en) 2003-04-03 2011-01-11 Fluidigm Corporation Thermal reaction device and method for using the same
EP1998884A4 (fr) * 2006-03-16 2011-05-18 Visiongate Inc Appareil à injecteur de flux coaxial en porte-à-faux et procédé de tri de particules
US7963399B2 (en) 2002-04-17 2011-06-21 Cytonome/St, Llc Method and apparatus for sorting particles
US8007746B2 (en) 2003-04-03 2011-08-30 Fluidigm Corporation Microfluidic devices and methods of using same
US8623295B2 (en) 2002-04-17 2014-01-07 Cytonome/St, Llc Microfluidic system including a bubble valve for regulating fluid flow through a microchannel
US8658418B2 (en) 2002-04-01 2014-02-25 Fluidigm Corporation Microfluidic particle-analysis systems
WO2014094791A1 (fr) * 2012-12-20 2014-06-26 Danmarks Tekniske Universitet Dispositif et méthode d'analyse de composition de fluide
US8828663B2 (en) 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
US8871446B2 (en) 2002-10-02 2014-10-28 California Institute Of Technology Microfluidic nucleic acid analysis
US9157550B2 (en) 2009-01-05 2015-10-13 The Board Of Trustees Of The University Of Illinois Microfluidic systems and methods
US9339850B2 (en) 2002-04-17 2016-05-17 Cytonome/St, Llc Method and apparatus for sorting particles
US9714443B2 (en) 2002-09-25 2017-07-25 California Institute Of Technology Microfabricated structure having parallel and orthogonal flow channels controlled by row and column multiplexors
US9943847B2 (en) 2002-04-17 2018-04-17 Cytonome/St, Llc Microfluidic system including a bubble valve for regulating fluid flow through a microchannel
US10994273B2 (en) 2004-12-03 2021-05-04 Cytonome/St, Llc Actuation of parallel microfluidic arrays

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0177718A2 (fr) * 1984-09-11 1986-04-16 Partec AG Procédé et dispositif pour le triage de particules microscopiques
US4676274A (en) * 1985-02-28 1987-06-30 Brown James F Capillary flow control

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0177718A2 (fr) * 1984-09-11 1986-04-16 Partec AG Procédé et dispositif pour le triage de particules microscopiques
US4676274A (en) * 1985-02-28 1987-06-30 Brown James F Capillary flow control

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
PROCEEDINGS OF THE IEEE. vol. 70, no. 5, May 1982, NEW YORK US pages 420 - 457; K E PETERSEN: 'Silicon as a mechanical material ' see page 434 - page 435 see page 442 - page 443 see page 446 - page 449 *
REVIEW OF SCIENTIFIC INSTRUMENTS. vol. 51, no. 1, January 1980, NEW YORK US pages 111 - 115; P LENZ: 'Mechanical cell separator ' see page 111 - page 113 *

Cited By (92)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5955029A (en) * 1992-05-01 1999-09-21 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US5635358A (en) * 1992-05-01 1997-06-03 Trustees Of The University Of Pennsylvania Fluid handling methods for use in mesoscale analytical devices
US7494770B2 (en) 1992-05-01 2009-02-24 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification analysis
US5427946A (en) * 1992-05-01 1995-06-27 Trustees Of The University Of Pennsylvania Mesoscale sperm handling devices
US5928880A (en) * 1992-05-01 1999-07-27 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US5486335A (en) * 1992-05-01 1996-01-23 Trustees Of The University Of Pennsylvania Analysis based on flow restriction
US5498392A (en) * 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US6660517B1 (en) 1992-05-01 2003-12-09 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5587128A (en) * 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US6184029B1 (en) 1992-05-01 2001-02-06 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US5726026A (en) * 1992-05-01 1998-03-10 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US5744366A (en) * 1992-05-01 1998-04-28 Trustees Of The University Of Pennsylvania Mesoscale devices and methods for analysis of motile cells
US5304487A (en) * 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
WO1993022054A1 (fr) * 1992-05-01 1993-11-11 Trustees Of The University Of Pennsylvania Analyse fondee sur un resserrement du systeme d'ecoulment
US6953676B1 (en) 1992-05-01 2005-10-11 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US7892819B2 (en) 1992-05-01 2011-02-22 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5480614A (en) * 1993-03-16 1996-01-02 Hitachi, Ltd. Micro-reactor device for minute sample analysis
EP0616218A1 (fr) * 1993-03-16 1994-09-21 Hitachi, Ltd. Dispositif micro-réacteur et système d'analyse de très petits échantillons utilisant ce dispositif
US5950842A (en) * 1995-02-23 1999-09-14 Natec, Reich, Summer Gmbh & Co. Kg Line connector with 90 degree rotation mechanism
DE19520298A1 (de) * 1995-06-02 1996-12-05 Bayer Ag Sortiervorrichtung für biologische Zellen oder Viren
US5837200A (en) * 1995-06-02 1998-11-17 Bayer Aktiengesellschaft Sorting device for biological cells or viruses
US5906732A (en) * 1995-11-17 1999-05-25 Yazaki Corporation Apparatus for separating minute substances in liquid
US5747349A (en) * 1996-03-20 1998-05-05 University Of Washington Fluorescent reporter beads for fluid analysis
US7138269B2 (en) 1996-09-04 2006-11-21 Inverness Medical Switzerland Gmbh Microflow system for particle separation and analysis
US6432630B1 (en) 1996-09-04 2002-08-13 Scandinanian Micro Biodevices A/S Micro-flow system for particle separation and analysis
US6056860A (en) * 1996-09-18 2000-05-02 Aclara Biosciences, Inc. Surface modified electrophoretic chambers
US6332540B1 (en) 1997-04-08 2001-12-25 Smithkline Beecham P.L.C. Device for isolating small polymeric beads from a suspension of such beads
WO1998045683A1 (fr) * 1997-04-08 1998-10-15 Smithkline Beecham Plc Dispositif servant a isoler de petites perles polymeres depuis une suspension de ces perles
US6540895B1 (en) * 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
WO1999061888A3 (fr) * 1998-05-22 2001-12-13 California Inst Of Techn Trieur de cellules microfabrique
AU763433B2 (en) * 1998-05-22 2003-07-24 California Institute Of Technology Microfabricated cell sorter
US6193647B1 (en) 1999-04-08 2001-02-27 The Board Of Trustees Of The University Of Illinois Microfluidic embryo and/or oocyte handling device and method
US6695765B1 (en) 1999-04-08 2004-02-24 The Board Of Trustees Of The University Of Illinois Microfluidic channel embryo and/or oocyte handling, analysis and biological evaluation
WO2001019516A1 (fr) * 1999-09-13 2001-03-22 Hoffmann La Roche Systeme de manipulation de suspensions
WO2001049412A1 (fr) * 1999-12-30 2001-07-12 Acreo Ab Procede et dispositif de regulation de debit
US7622081B2 (en) 2000-06-05 2009-11-24 California Institute Of Technology Integrated active flux microfluidic devices and methods
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
US8129176B2 (en) 2000-06-05 2012-03-06 California Institute Of Technology Integrated active flux microfluidic devices and methods
US8257666B2 (en) 2000-06-05 2012-09-04 California Institute Of Technology Integrated active flux microfluidic devices and methods
WO2002001189A1 (fr) * 2000-06-26 2002-01-03 Gnothis Holding S.A. Procede de selection de particules
DE10031028B4 (de) * 2000-06-26 2008-09-04 Gnothis Holding Sa Verfahren zur Selektion von Partikeln
US9926521B2 (en) 2000-06-27 2018-03-27 Fluidigm Corporation Microfluidic particle-analysis systems
US8936764B2 (en) 2001-04-06 2015-01-20 California Institute Of Technology Nucleic acid amplification using microfluidic devices
US8486636B2 (en) 2001-04-06 2013-07-16 California Institute Of Technology Nucleic acid amplification using microfluidic devices
US7833708B2 (en) 2001-04-06 2010-11-16 California Institute Of Technology Nucleic acid amplification using microfluidic devices
WO2003025563A1 (fr) * 2001-09-16 2003-03-27 Chemometec A/S Procede et systeme de detection et eventuellement d'isolement de particules peu abondantes
US8163492B2 (en) 2001-11-30 2012-04-24 Fluidign Corporation Microfluidic device and methods of using same
US8343442B2 (en) 2001-11-30 2013-01-01 Fluidigm Corporation Microfluidic device and methods of using same
US7118910B2 (en) 2001-11-30 2006-10-10 Fluidigm Corporation Microfluidic device and methods of using same
US7820427B2 (en) 2001-11-30 2010-10-26 Fluidigm Corporation Microfluidic device and methods of using same
US9643178B2 (en) 2001-11-30 2017-05-09 Fluidigm Corporation Microfluidic device with reaction sites configured for blind filling
US7837946B2 (en) 2001-11-30 2010-11-23 Fluidigm Corporation Microfluidic device and methods of using same
US7691333B2 (en) 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
US7452725B2 (en) 2002-01-10 2008-11-18 Board Of Regents, The University Of Texas System Flow sorting system and methods regarding same
WO2003060486A1 (fr) * 2002-01-10 2003-07-24 Board Of Regents, The University Of Texas System Systeme de tri de flux et procedes associes
WO2003078972A1 (fr) * 2002-03-14 2003-09-25 Micronics, Inc. Cytometrie en flux sur ruban et triage de cellules
WO2003078065A1 (fr) * 2002-03-14 2003-09-25 Micronics, Inc. Dispositif microfluidique a reseau de canaux
US7223371B2 (en) 2002-03-14 2007-05-29 Micronics, Inc. Microfluidic channel network device
US7452726B2 (en) 2002-04-01 2008-11-18 Fluidigm Corporation Microfluidic particle-analysis systems
US8658418B2 (en) 2002-04-01 2014-02-25 Fluidigm Corporation Microfluidic particle-analysis systems
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US9339850B2 (en) 2002-04-17 2016-05-17 Cytonome/St, Llc Method and apparatus for sorting particles
US9550215B2 (en) 2002-04-17 2017-01-24 Cytonome/St, Llc Method and apparatus for sorting particles
US10427159B2 (en) 2002-04-17 2019-10-01 Cytonome/St, Llc Microfluidic device
US10029263B2 (en) 2002-04-17 2018-07-24 Cytonome/St, Llc Method and apparatus for sorting particles
US9011797B2 (en) 2002-04-17 2015-04-21 Cytonome/St, Llc Microfluidic system including a bubble valve for regulating fluid flow through a microchannel
US7963399B2 (en) 2002-04-17 2011-06-21 Cytonome/St, Llc Method and apparatus for sorting particles
US8623295B2 (en) 2002-04-17 2014-01-07 Cytonome/St, Llc Microfluidic system including a bubble valve for regulating fluid flow through a microchannel
US10710120B2 (en) 2002-04-17 2020-07-14 Cytonome/St, Llc Method and apparatus for sorting particles
US10029283B2 (en) 2002-04-17 2018-07-24 Cytonome/St, Llc Method and apparatus for sorting particles
US9943847B2 (en) 2002-04-17 2018-04-17 Cytonome/St, Llc Microfluidic system including a bubble valve for regulating fluid flow through a microchannel
EP1499453A4 (fr) * 2002-04-17 2009-11-04 Cytonome Inc Procede et appareil de tri de particules
US11027278B2 (en) 2002-04-17 2021-06-08 Cytonome/St, Llc Methods for controlling fluid flow in a microfluidic system
EP1567845A4 (fr) * 2002-07-08 2007-07-18 John S Foster Procede et appareil destines a trier des cellules biologiques au moyen d'un dispositif a systeme mecanique microelectrique
US9714443B2 (en) 2002-09-25 2017-07-25 California Institute Of Technology Microfabricated structure having parallel and orthogonal flow channels controlled by row and column multiplexors
US10328428B2 (en) 2002-10-02 2019-06-25 California Institute Of Technology Apparatus for preparing cDNA libraries from single cells
US9579650B2 (en) 2002-10-02 2017-02-28 California Institute Of Technology Microfluidic nucleic acid analysis
US8871446B2 (en) 2002-10-02 2014-10-28 California Institute Of Technology Microfluidic nucleic acid analysis
US10940473B2 (en) 2002-10-02 2021-03-09 California Institute Of Technology Microfluidic nucleic acid analysis
US8247178B2 (en) 2003-04-03 2012-08-21 Fluidigm Corporation Thermal reaction device and method for using the same
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
US7749737B2 (en) 2003-04-03 2010-07-06 Fluidigm Corporation Thermal reaction device and method for using the same
US8007746B2 (en) 2003-04-03 2011-08-30 Fluidigm Corporation Microfluidic devices and methods of using same
US7867454B2 (en) 2003-04-03 2011-01-11 Fluidigm Corporation Thermal reaction device and method for using the same
US10131934B2 (en) 2003-04-03 2018-11-20 Fluidigm Corporation Thermal reaction device and method for using the same
US10994273B2 (en) 2004-12-03 2021-05-04 Cytonome/St, Llc Actuation of parallel microfluidic arrays
US8828663B2 (en) 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
WO2007084392A3 (fr) * 2006-01-13 2007-11-08 Micronics Inc Soupapes a actionnement electromagnetique a utiliser dans des structures microfluidiques
EP1998884A4 (fr) * 2006-03-16 2011-05-18 Visiongate Inc Appareil à injecteur de flux coaxial en porte-à-faux et procédé de tri de particules
US9157550B2 (en) 2009-01-05 2015-10-13 The Board Of Trustees Of The University Of Illinois Microfluidic systems and methods
WO2014094791A1 (fr) * 2012-12-20 2014-06-26 Danmarks Tekniske Universitet Dispositif et méthode d'analyse de composition de fluide
US9557258B2 (en) 2012-12-20 2017-01-31 Danmarks Tekniske Universitet Fluid composition analysis device and method

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