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WO1991013075A3 - Procede et reactif permettant de determiner des variations specifique de nucleotides - Google Patents

Procede et reactif permettant de determiner des variations specifique de nucleotides Download PDF

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Publication number
WO1991013075A3
WO1991013075A3 PCT/FI1991/000046 FI9100046W WO9113075A3 WO 1991013075 A3 WO1991013075 A3 WO 1991013075A3 FI 9100046 W FI9100046 W FI 9100046W WO 9113075 A3 WO9113075 A3 WO 9113075A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection step
incorporation
variable nucleotide
reagent
primer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FI1991/000046
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English (en)
Other versions
WO1991013075A2 (fr
Inventor
Hans Soederlund
Ann-Christine Syvaenen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Orion Oyj
Original Assignee
Orion Yhtyma Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to EP91903942A priority Critical patent/EP0648280B1/fr
Priority to DK91903942T priority patent/DK0648280T3/da
Priority to CA002071537A priority patent/CA2071537C/fr
Priority to DE69131233T priority patent/DE69131233T2/de
Priority to DE0648280T priority patent/DE648280T1/de
Application filed by Orion Yhtyma Oy filed Critical Orion Yhtyma Oy
Publication of WO1991013075A2 publication Critical patent/WO1991013075A2/fr
Publication of WO1991013075A3 publication Critical patent/WO1991013075A3/fr
Priority to NO19923116A priority patent/NO311529B1/no
Priority to FI923653A priority patent/FI102297B/fi
Anticipated expiration legal-status Critical
Priority to GR950300047T priority patent/GR950300047T1/el
Priority to GR990401879T priority patent/GR3030790T3/el
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • AIDS & HIV (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Saccharide Compounds (AREA)

Abstract

La détection de nucléotide(s) variable(s) se fonde sur l'extension des amorces et sur l'incorporation de triphosphates de nucléosides détectables. En sélectionnant les amorces de la phase de détection à partir de la région immédiatement adjacente au nucléotide variable, on peut détecter cette variation déjà après l'incorporation d'un seul triphosphate de nucléoside. Des triphosphates de nucléosides marqués correspondant au nucléotide variable sont ajoutés et l'incorporation d'une marque dans l'amorce de la phase de détection est mesurée. La sélection de l'amorce de la phase de détection est importante pour la réalisation du procédé de la présente invention et dépend de la séquence de nucléotides d'intérêt. Les amorces de la phase de détection sont de préférence sélectionnées de façon à couvrir la région s'étendant immédiatement du nucléotide variable à détecter à l'extrémité 3'. Un tel procédé sert à identifier des mutations ponctuelles et des variations génétiques spécifiques.
PCT/FI1991/000046 1990-02-16 1991-02-15 Procede et reactif permettant de determiner des variations specifique de nucleotides Ceased WO1991013075A2 (fr)

Priority Applications (9)

Application Number Priority Date Filing Date Title
EP91903942A EP0648280B1 (fr) 1990-02-16 1991-02-15 Procede et reactif permettant de determiner des variations specifique de nucleotides
DK91903942T DK0648280T3 (da) 1990-02-16 1991-02-15 Fremgangsmåde og reagenser til bestemmelse af specifikke nucleotidvariationer
CA002071537A CA2071537C (fr) 1990-02-16 1991-02-15 Methode et reactif permettant de determiner certaines variations dans les nucleotides
DE69131233T DE69131233T2 (de) 1990-02-16 1991-02-15 Verfahren und reagens zum nachweis von spezifischen nukleotid-variationen
DE0648280T DE648280T1 (de) 1990-02-16 1991-02-15 Verfahren und reagens zum nachweis von spezifischen nukleotid-variationen.
NO19923116A NO311529B1 (no) 1990-02-16 1992-08-10 Fremgangsmåte og reagens for bestemmelse av spesifikke nukleotidvariasjoner
FI923653A FI102297B (fi) 1990-02-16 1992-08-14 Menetelmä ja reagenssi spesifisten nukleotidivariaatioiden määrittämis eksi
GR950300047T GR950300047T1 (en) 1990-02-16 1995-07-31 Method and reagent for determining specific nucleotide variations.
GR990401879T GR3030790T3 (en) 1990-02-16 1999-07-14 Method and reagent for determining specific nucleotide variations.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48200590A 1990-02-16 1990-02-16
US482,005 1990-02-16

Publications (2)

Publication Number Publication Date
WO1991013075A2 WO1991013075A2 (fr) 1991-09-05
WO1991013075A3 true WO1991013075A3 (fr) 1991-10-17

Family

ID=23914258

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FI1991/000046 Ceased WO1991013075A2 (fr) 1990-02-16 1991-02-15 Procede et reactif permettant de determiner des variations specifique de nucleotides

Country Status (18)

Country Link
EP (1) EP0648280B1 (fr)
JP (1) JP2786011B2 (fr)
AT (1) ATE180019T1 (fr)
AU (1) AU642709B2 (fr)
CA (1) CA2071537C (fr)
DE (2) DE648280T1 (fr)
DK (1) DK0648280T3 (fr)
ES (1) ES2072235T3 (fr)
FI (1) FI102297B (fr)
GR (2) GR950300047T1 (fr)
HU (1) HU211058B (fr)
IE (1) IE910525A1 (fr)
IL (1) IL97222A (fr)
NO (1) NO311529B1 (fr)
NZ (1) NZ237134A (fr)
PT (1) PT96776B (fr)
WO (1) WO1991013075A2 (fr)
ZA (1) ZA911152B (fr)

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US7125668B2 (en) 1996-11-05 2006-10-24 Clinical Micro Sensors, Inc. Electrodes linked via conductive oligomers to nucleic acids
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US7384749B2 (en) 1996-11-05 2008-06-10 Clinical Micro Sensors, Inc. Electrodes linked via conductive oligomers to nucleic acids
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US7851146B2 (en) 1993-12-10 2010-12-14 California Institute Of Technology Nucleic acid mediated electron transfer
USRE44693E1 (en) 1996-11-06 2014-01-07 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
US9243284B2 (en) 2000-12-01 2016-01-26 Life Technologies Corporation Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis
US9279148B2 (en) 1999-04-20 2016-03-08 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
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JPH05504477A (ja) 1993-07-15
HUT61330A (en) 1992-12-28
PT96776A (pt) 1991-10-31
DK0648280T3 (da) 1999-11-01
PT96776B (pt) 1998-05-29
JP2786011B2 (ja) 1998-08-13
FI923653L (fi) 1992-08-14
DE69131233D1 (de) 1999-06-17
NZ237134A (en) 1992-06-25
NO923116D0 (no) 1992-08-10
DE69131233T2 (de) 1999-11-04
AU642709B2 (en) 1993-10-28
GR3030790T3 (en) 1999-11-30
HU910516D0 (en) 1991-09-30
IL97222A0 (en) 1992-05-25
WO1991013075A2 (fr) 1991-09-05
IL97222A (en) 1995-08-31
ES2072235T1 (es) 1995-07-16
HU211058B (en) 1995-10-30
ZA911152B (en) 1991-11-27
AU7235191A (en) 1991-09-18
EP0648280A1 (fr) 1995-04-19
FI102297B1 (fi) 1998-11-13
FI923653A0 (fi) 1992-08-14
DE648280T1 (de) 1995-11-30
CA2071537C (fr) 2003-02-11
FI102297B (fi) 1998-11-13
EP0648280B1 (fr) 1999-05-12
NO311529B1 (no) 2001-12-03
ES2072235T3 (es) 1999-07-16
GR950300047T1 (en) 1995-07-31
NO923116L (no) 1992-08-10
IE910525A1 (en) 1991-08-28
ATE180019T1 (de) 1999-05-15
CA2071537A1 (fr) 1991-08-17

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