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WO1991011531A1 - SONDE D'HYBRIDATION POUR LA DETECTION DES SOUCHES DE $i(BRANHAMELLA CATARRHALIS) - Google Patents

SONDE D'HYBRIDATION POUR LA DETECTION DES SOUCHES DE $i(BRANHAMELLA CATARRHALIS) Download PDF

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Publication number
WO1991011531A1
WO1991011531A1 PCT/EP1991/000211 EP9100211W WO9111531A1 WO 1991011531 A1 WO1991011531 A1 WO 1991011531A1 EP 9100211 W EP9100211 W EP 9100211W WO 9111531 A1 WO9111531 A1 WO 9111531A1
Authority
WO
WIPO (PCT)
Prior art keywords
probes
group
hybridization
branhamella catarrhalis
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP1991/000211
Other languages
English (en)
Inventor
Rudi Rossau
Hugo Van Heuverswyn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujirebio Europe NV SA
Original Assignee
Innogenetics NV SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Innogenetics NV SA filed Critical Innogenetics NV SA
Publication of WO1991011531A1 publication Critical patent/WO1991011531A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Definitions

  • Probes targeting rRNA are advantageous over probes which target the genomic DNA such as the probe described by Beaulieu and Roy, since rRNA is single- stranded and consequently directly available for hybridization, and is present in multiple copies in bacterial cells.
  • the invention thus provides for probes which are either replicas (those designated by numbers followed by "IC” or “ICR”) in terms of nucleotide sequence of sequences contained in the RNAs or DNAs of most Branhamella catarrhalis strains with occasionally a few insignificant differences in nucleotide sequences or formed of sequences, those designated by bare numbers or by numbers followed by "R”, complementary to sequences included in the natural RNAs of Branhamella catarrhalis.
  • the 200 ⁇ l hybridization mix containing 2 pmol digoxigenine labeled probe BCl was added and hybridization was done at 60"C for 1 h. Washing was performed at the same stringency for 20 min.
  • the detection protocol as described in the Boehringer labeling and detection kit was followed except that paranitrophenyl-phosphate (3.6 mg/ l) was used as substrate for alkaline phosphatase.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des sondes d'hybridation servant à détecter les souches de Branhamella catarrhalis. Les sondes faisant l'objet de l'invention sont caractérisées par les séquences de nucléotides (I) suivantes:
PCT/EP1991/000211 1990-02-02 1991-02-01 SONDE D'HYBRIDATION POUR LA DETECTION DES SOUCHES DE $i(BRANHAMELLA CATARRHALIS) Ceased WO1991011531A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB90400297.9 1990-02-02
EP90400297 1990-02-02

Publications (1)

Publication Number Publication Date
WO1991011531A1 true WO1991011531A1 (fr) 1991-08-08

Family

ID=8205679

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1991/000211 Ceased WO1991011531A1 (fr) 1990-02-02 1991-02-01 SONDE D'HYBRIDATION POUR LA DETECTION DES SOUCHES DE $i(BRANHAMELLA CATARRHALIS)

Country Status (3)

Country Link
AU (1) AU7188491A (fr)
CA (1) CA2075186A1 (fr)
WO (1) WO1991011531A1 (fr)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0759777A4 (fr) * 1994-05-17 1998-12-02 Univ New York State Res Found VACCIN CONTRE -i(MORAXELLA CATARRHALIS)
EP0737085A4 (fr) * 1993-09-29 1999-04-07 Univ New York State Res Found VACCIN CONTRE -i(BRANHAMELLA CATARRHALIS)
US5994066A (en) * 1995-09-11 1999-11-30 Infectio Diagnostic, Inc. Species-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
US6001564A (en) * 1994-09-12 1999-12-14 Infectio Diagnostic, Inc. Species specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
US6294667B1 (en) 1996-10-07 2001-09-25 Amersham International Plc Analysis of carbohydrates
US7943346B2 (en) 1994-09-12 2011-05-17 Geneohm Sciences Canada Inc. Probes and primers for detection of bacterial pathogens and antibiotic resistance genes
US8034588B2 (en) 1997-11-04 2011-10-11 Geneohm Sciences Canada Inc. Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories
US8114601B2 (en) 1999-09-28 2012-02-14 Geneohm Sciences Canada Inc. Highly conserved genes and their use to generate probes and primers for detection of microorganisms
US8426137B2 (en) 1996-11-04 2013-04-23 Genohm Sciences Canada, Inc. Methods and probes for detecting a vancomycin resistance gene

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0079139A1 (fr) * 1981-10-16 1983-05-18 Orion-yhtymä Oy Méthode et combinaison de réactifs pour l'identification des micro-organismes et l'utilisation de la hybridisation en sandwich des acides nucléiques à cette fin

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0079139A1 (fr) * 1981-10-16 1983-05-18 Orion-yhtymä Oy Méthode et combinaison de réactifs pour l'identification des micro-organismes et l'utilisation de la hybridisation en sandwich des acides nucléiques à cette fin

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Abstracts of the 89th Annual Meeting of the American Society for Microbology, New Orleans LA, 14th-18th May 1989 vol. 89(D) D. Beaulieu et al.: "Construction of a species-specific DNA probe for Branhamella catarrhalis", see page 124 *
Journal of General Microbology, vol. 135, 1989 SGM (Colchester, GB) R.Rosseau et al.: "Specific Neisseria gonorrhoeae DNA-probes derived from ribosomal RNA", pages 1735-1745 *
Nucleic Acids Research, vol. 17, no. 19, 11 october 1989, IRL Press (Oxford, GB) U. Edwards et al.: "Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16s ribosomal RNA", pages 7843-7853 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0737085A4 (fr) * 1993-09-29 1999-04-07 Univ New York State Res Found VACCIN CONTRE -i(BRANHAMELLA CATARRHALIS)
EP0759777A4 (fr) * 1994-05-17 1998-12-02 Univ New York State Res Found VACCIN CONTRE -i(MORAXELLA CATARRHALIS)
US6001564A (en) * 1994-09-12 1999-12-14 Infectio Diagnostic, Inc. Species specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
US7943346B2 (en) 1994-09-12 2011-05-17 Geneohm Sciences Canada Inc. Probes and primers for detection of bacterial pathogens and antibiotic resistance genes
US5994066A (en) * 1995-09-11 1999-11-30 Infectio Diagnostic, Inc. Species-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
US6294667B1 (en) 1996-10-07 2001-09-25 Amersham International Plc Analysis of carbohydrates
US8426137B2 (en) 1996-11-04 2013-04-23 Genohm Sciences Canada, Inc. Methods and probes for detecting a vancomycin resistance gene
US8034588B2 (en) 1997-11-04 2011-10-11 Geneohm Sciences Canada Inc. Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories
US8067207B2 (en) 1997-11-04 2011-11-29 Geneohm Sciences Canada Inc. Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories
US8114601B2 (en) 1999-09-28 2012-02-14 Geneohm Sciences Canada Inc. Highly conserved genes and their use to generate probes and primers for detection of microorganisms
US8182996B2 (en) 1999-09-28 2012-05-22 Geneohm Sciences Canada Inc. Compositions and methods for detecting Klebsiella pneumoniae
US10047404B2 (en) 1999-09-28 2018-08-14 Geneohm Sciences Canada, Inc. Highly conserved tuf genes and their use to generate probes and primers for detection of coagulase-negative Staphylococcus

Also Published As

Publication number Publication date
AU7188491A (en) 1991-08-21
CA2075186A1 (fr) 1991-08-03

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