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WO1991009944A3 - Transcriptases inverses obtenues a haute temperature - Google Patents

Transcriptases inverses obtenues a haute temperature Download PDF

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Publication number
WO1991009944A3
WO1991009944A3 PCT/US1990/007641 US9007641W WO9109944A3 WO 1991009944 A3 WO1991009944 A3 WO 1991009944A3 US 9007641 W US9007641 W US 9007641W WO 9109944 A3 WO9109944 A3 WO 9109944A3
Authority
WO
WIPO (PCT)
Prior art keywords
high temperature
reverse transcriptases
temperature reverse
reverse transcription
rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1990/007641
Other languages
English (en)
Other versions
WO1991009944A2 (fr
Inventor
David H Gelfand
Thomas W Myers
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Original Assignee
Cetus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27412639&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1991009944(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US07/455,611 external-priority patent/US5322770A/en
Application filed by Cetus Corp filed Critical Cetus Corp
Priority to CA002071213A priority Critical patent/CA2071213C/fr
Priority to DE69030386T priority patent/DE69030386T2/de
Priority to EP91904087A priority patent/EP0506889B1/fr
Priority to AT91904087T priority patent/ATE151112T1/de
Priority to AU72444/91A priority patent/AU656315B2/en
Publication of WO1991009944A2 publication Critical patent/WO1991009944A2/fr
Publication of WO1991009944A3 publication Critical patent/WO1991009944A3/fr
Anticipated expiration legal-status Critical
Priority to US08/082,182 priority patent/US5310652A/en
Priority to GR970401505T priority patent/GR3023862T3/el
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Procédés de réplication et d'amplification de séquences d'ARN à l'aide de polymérases d'ADN thermoactives. La transcription inverse d'ARN est catalysée par, par exemple, la polymérase d'ADN de Taq de 94 kDa, de Taq de 62 kDa, de nTth et de Tth recombiné. La transcription inverse est couplée à l'amplification par réaction en chaîne de polymérases (RCP) dans une procédure mettant en ÷uvre une seule enzyme, utilisant une polymérase thermostable.
PCT/US1990/007641 1986-08-22 1990-12-21 Transcriptases inverses obtenues a haute temperature Ceased WO1991009944A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CA002071213A CA2071213C (fr) 1989-12-22 1990-12-21 Transcriptases inverses de haute temperature
DE69030386T DE69030386T2 (de) 1989-12-22 1990-12-21 Bei hoher temperatur aktive reverse transkriptasen
EP91904087A EP0506889B1 (fr) 1989-12-22 1990-12-21 Transcriptases inverses obtenues a haute temperature
AT91904087T ATE151112T1 (de) 1989-12-22 1990-12-21 Bei hoher temperatur aktive reverse transkriptasen
AU72444/91A AU656315B2 (en) 1989-12-22 1990-12-21 pigh temperature reverse transcriptases
US08/082,182 US5310652A (en) 1986-08-22 1993-06-24 Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
GR970401505T GR3023862T3 (en) 1989-12-22 1997-06-24 High temperature reverse transcriptases

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US45596789A 1989-12-22 1989-12-22
US455,967 1989-12-22
US455,611 1989-12-22
US07/455,611 US5322770A (en) 1989-12-22 1989-12-22 Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US58547190A 1990-09-20 1990-09-20
US585,471 1990-09-20

Publications (2)

Publication Number Publication Date
WO1991009944A2 WO1991009944A2 (fr) 1991-07-11
WO1991009944A3 true WO1991009944A3 (fr) 1991-08-22

Family

ID=27412639

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1990/007641 Ceased WO1991009944A2 (fr) 1986-08-22 1990-12-21 Transcriptases inverses obtenues a haute temperature

Country Status (10)

Country Link
EP (1) EP0506889B1 (fr)
JP (1) JP2968585B2 (fr)
AT (1) ATE151112T1 (fr)
AU (1) AU656315B2 (fr)
CA (1) CA2071213C (fr)
DE (1) DE69030386T2 (fr)
DK (1) DK0506889T3 (fr)
ES (1) ES2100945T3 (fr)
GR (1) GR3023862T3 (fr)
WO (1) WO1991009944A2 (fr)

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US5561058A (en) * 1986-08-22 1996-10-01 Hoffmann-La Roche Inc. Methods for coupled high temperatures reverse transcription and polymerase chain reactions
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5795762A (en) * 1986-08-22 1998-08-18 Roche Molecular Systems, Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5693517A (en) * 1987-06-17 1997-12-02 Roche Molecular Systems, Inc. Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
EP0550687B1 (fr) * 1990-09-28 1999-06-09 F. Hoffmann-La Roche Ag Mutations d'adn-polymerases thermostables en 5' a 3' exonuclease
AU8906091A (en) * 1990-10-05 1992-04-28 Wayne M. Barnes Thermostable dna polymerase
CA2038839A1 (fr) * 1991-03-22 1992-09-23 Jiang J. Liu Methode de detection de l'adn viral
AU662906B2 (en) * 1991-06-26 1995-09-21 F. Hoffmann-La Roche Ag Methods for detection of carcinoma metastases by nucleic acid amplification
EP0787807B1 (fr) * 1991-08-27 2003-05-28 F. Hoffmann-La Roche Ag Amorces et sondes pour la détection de l'hépatite C
FR2690691B1 (fr) * 1992-04-29 1999-02-12 Bio Merieux Procede d'amplification d'arn necessitant une seule etape de manipulation.
USRE39031E1 (en) * 1992-04-29 2006-03-21 Biomerieux RNA amplification method requiring only one manipulation step
DE69428167T2 (de) * 1993-07-01 2002-05-29 F. Hoffmann-La Roche Ag, Basel Reagenz und Verfahren für reverse Transkription bei hoher Temperatur, verbunden mit einer Polymerase-Kettenreaktion
US5474920A (en) * 1993-11-23 1995-12-12 State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University Modified thermo-resistant DNA polymerases
EP0682121B1 (fr) * 1993-12-01 2002-08-28 Toyo Boseki Kabushiki Kaisha Procede d'amplification et de detection d'une sequence nucleotidique au moyen d'enzymes thermostables
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
EP0736609A3 (fr) * 1995-04-08 1998-07-29 Roche Diagnostics GmbH Procédé pour l'amplification spécifique et pour la détection d'ADN ou d'ARN
CA2222744C (fr) * 1995-05-31 2008-03-25 Amersham Life Science, Inc. Adn-polymerases thermostables
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
EP0866868B1 (fr) * 1995-12-15 2001-07-11 Amersham Life Science Inc Polymerase d'adn thermostable issue de thermoanaerobacter thermohydrosulfuricus et enzymes mutants a activite d'exonuclease supprimee ainsi obtenus
CA2199213C (fr) * 1996-03-11 2007-04-17 John Wesley Backus Amplification et detection des acides nucleiques cibles grace a une etape d'incubation post-amplification
US6013488A (en) * 1996-07-25 2000-01-11 The Institute Of Physical And Chemical Research Method for reverse transcription
ES2121698B1 (es) * 1996-12-02 1999-07-01 Inia Procedimiento para la obtencionn de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna.
US6030814A (en) * 1997-04-21 2000-02-29 Epicentre Technologies Corporation Reverse transcription method
AU7206498A (en) * 1997-05-13 1998-12-08 Display Systems Biotech A/S A method to clone mrnas and display of differentially expressed transcripts (dodet)
EP0922765B1 (fr) * 1997-12-02 2005-11-23 Roche Diagnostics GmbH Polymérase d'ADN modifiée de Carboxydothermus hydrogenoformans et son utilisation pour transcription inverse suivi par la réaction en chaíne de la polymérase
EP0921196A1 (fr) * 1997-12-02 1999-06-09 Roche Diagnostics GmbH Polymérase d'ADN modifiée de Carboxydothermus hydrogenoformans et son utilisation pour transcription inverse suivi par la réaction en chaíne de la polymérase
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AT410218B (de) 1999-08-20 2003-03-25 Baxter Ag Verfahren zur herstellung eines qualitätsgesicherten pools biologischer proben
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US7575864B2 (en) * 2004-05-27 2009-08-18 E.I. Du Pont De Nemours And Company Method for the direct detection of diagnostic RNA
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JP5191041B2 (ja) 2007-04-05 2013-04-24 エフ．ホフマン−ラ ロシュ アーゲー 急速ワンステップｒｔ−ｐｃｒ
KR101039521B1 (ko) * 2008-02-01 2011-06-08 주식회사 인트론바이오테크놀로지 금속 이온에 의해 발생하는 ｐｃｒ 저해 및 비특이 증폭을완화시키거나 막을 수 있는 에틸렌글리콜테트라아세틱 산을킬레이트제로 포함하는 ｐｃｒ 반응용 조성물
CA2836117C (fr) 2009-12-10 2017-08-15 Ottawa Hospital Research Institute Rhabdovirus oncolitique
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JP2016513115A (ja) 2013-02-21 2016-05-12 チルドレンズ ホスピタル オブ イースタン オンタリオ リサーチ インスティチュート インコーポレイテッド ワクチン組成物
EP3575411A1 (fr) 2014-01-31 2019-12-04 QIAGEN GmbH Démarrage à chaud pour chélateur cationique
WO2016160965A1 (fr) 2015-03-30 2016-10-06 Rubicon Genomics, Inc. Procédés et compositions permettant la réparation des extrémités de l'adn par de multiples activités enzymatiques
US11236384B2 (en) 2017-01-19 2022-02-01 Asuragen, Inc. Methods of RNA amplification
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CN110846724B (zh) * 2019-12-03 2023-04-28 江西海普洛斯医学检验实验室有限公司 构建mRNA链特异文库的方法及试剂盒
JP7580877B2 (ja) * 2020-03-30 2024-11-12 国立研究開発法人産業技術総合研究所 核酸増幅用組成物
CN113045410A (zh) * 2021-03-29 2021-06-29 华东理工大学 一种双环降二萜类化合物及其合成基因及制备方法
EP4074839A1 (fr) 2021-04-16 2022-10-19 Biotype GmbH Sonde tx oligonucléotidique optimisée pour une analyse de multiplexage d'acides nucléiques et méthode de multiplexage
EP4350002A1 (fr) 2022-10-07 2024-04-10 Biotype GmbH Detection of molecular analytes based on tailored probe competition
DE102023124346A1 (de) 2023-09-09 2025-03-13 Biotype Gmbh Verfahren zur Detektion des Endometriumkarzinoms Subtyp CN hoch

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Patent Citations (1)

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Non-Patent Citations (3)

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Title
Analytical Biochemistry, vol. 190, no. 2, 1 November 1990, Academic Press, Inc., A.L. Shaffer et al.: "Amplification, detection, and automated sequencing of gibbon interleukin-2 mRNA by Thermus aquaticus DNA polymerase reverse transcription and polymerase chain reaction", pages 292-296 *
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Also Published As

Publication number Publication date
DE69030386D1 (de) 1997-05-07
WO1991009944A2 (fr) 1991-07-11
EP0506889A1 (fr) 1992-10-07
AU7244491A (en) 1991-07-24
EP0506889B1 (fr) 1997-04-02
JP2968585B2 (ja) 1999-10-25
ATE151112T1 (de) 1997-04-15
AU656315B2 (en) 1995-02-02
JPH05505105A (ja) 1993-08-05
CA2071213C (fr) 2000-05-02
GR3023862T3 (en) 1997-09-30
DE69030386T2 (de) 1997-10-09
CA2071213A1 (fr) 1991-06-23
DK0506889T3 (da) 1997-09-22
ES2100945T3 (es) 1997-07-01

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