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WO1991006368A1 - Pieces a inserer dans les cupules de plaques de microtitrage - Google Patents

Pieces a inserer dans les cupules de plaques de microtitrage Download PDF

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Publication number
WO1991006368A1
WO1991006368A1 PCT/GB1990/001646 GB9001646W WO9106368A1 WO 1991006368 A1 WO1991006368 A1 WO 1991006368A1 GB 9001646 W GB9001646 W GB 9001646W WO 9106368 A1 WO9106368 A1 WO 9106368A1
Authority
WO
WIPO (PCT)
Prior art keywords
insert
well
well insert
microtitre plate
series
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB1990/001646
Other languages
English (en)
Inventor
Raymond Edwards
John Alexander Little
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of WO1991006368A1 publication Critical patent/WO1991006368A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof

Definitions

  • This invention relates to well inserts for microtitre plates.
  • Microtitre plates having a plurality of circular wells are extensively used in diagnostic assays.
  • a diagnostic reagent is coated, usually by passive absorption, onto the surface of a microtitre plate and measurement is made using some form of optical signal, such as fluorescence, luminescence or optical density.
  • the known microtitre plate requires mechanical strengh for rigidity, absence of optical interference and a suitable surface for coating of the reagent, however these requirements are often incompatible with each other.
  • An aim of the present invention is to utilize the rigidity and optical qualities of known microtitre plates while improving the surface properties for the coating reagent.
  • a well insert for a microtitre plate comprising a hollow tubular member, the internal and/or external surface of which shaped to provide an extended surface area for a reagent coated on the surface, and the surface chemistry of which allows coating or covalent coupling of reagents.
  • the insert is made of a plastics material such as polystyrene or polypropylene.
  • Figure 1 is a plan view of a microtitre plate fitted with well inserts according to the invention.
  • Figure 2 is a cross-section taken along the line 2-2 of Figure 1;
  • Figure 3 is a plan view and vertical cross- section of a first embodiment of a well insert;
  • Figure 4 is a plan view and a vertical cross- section of a second embodiment of a well insert
  • Figure 5 is a plan view and a vertical cross- section of a third embodiment of a well insert
  • Figure 6 is a plan view and a side elevation of a fourth embodiment of a well insert
  • Figure 7 is a plan view and a side elevation of a fifth embodiment of a well insert
  • Figure 8 is a side elevation of a sixth embodiment of a well insert.
  • Figure 9 is a side elevation of a seventh embodiment of a well insert.
  • Figure 1 illustrates a plan view of a microtitre plate 1 made of a plastics material which has mechanical strength and enables the passage of light beams with the absence of optical interference.
  • plastics material is polystyrene.
  • the present invention provides a hollow tubular insert 3 which is fitted in the well 2 and provides an extended surface area for coating of the reagent.
  • Such an insert may be made of plastics material such as polystyrene or polypropylene which is not constrained by requirements for optical clarity or mechanical strength.
  • the insert may be made from a wide variety of materials some of which are optically opaque. Materials may be suitable for covalent coupling of reagents e.g. cellulose.
  • reagents e.g. cellulose.
  • the insert 3 is coated with a diagnostic reagent and placed in a well 2 of the microtitre plate 1.
  • the insert is tubular with open ends and does not restrict or interfere with subsequent optical measurements taken by passing a beam of light 4 through the well 2 as used in the assay system.
  • Figures 3 to 9 illustrate various embodiments of well inserts.
  • Figure 3 illustrates in plan and side elevation an open ended tubular insert 3 extruded from a plastics material with ribs 5 extending vertically around the internal surface.
  • Figure 4 shows similar views to Figure 3 of a second embodiment of a well insert 3 which is extruded from plastics material and is formed with a zig-zag or pleated wall 6.
  • Figure 5 shows similar view to Figure 3 and 4 of a third embodiment of a tubular well insert 3 the inner wall being formed with raised surface bumps 7 arranged in vertical parallel lines.
  • Figure 6 shows a fourth embodiment of a well insert in plan and side elevation.
  • the tubular insert 3 has a series of external spaced hoop projections 8 which provide a liquid-tight seal between the insert and well wall.
  • Figure 7 shows similar views of a fifth embodiment of a well insert 3 with external vertical ribs 9 spaced around the outer periphery of the tubular insert which provide access for the test sample to the space between the insert and the well wall.
  • Figure 8 is a side elevation of a sixth embodiment of a well insert 3 constructed from a helically formed plastics strip 10.
  • Figure 9 is a similar view to Figure 8 of a seventh embodiment of a well insert formed as a tubular open rectangular mesh 11.
  • Embodiments of the well insert are illustrated in which the wall of the insert has an extended surface area which provide a means for enhanced coating of a reagent on its surface, prior to insertion in the well of a microtitre plate for the diagnostic test.
  • the inserts and microtitre plate may be suitably coloured to distinguish between different reagent batches.
  • the well inserts can also be used with non- optical detection, such as radioactivity.
  • the well insert is removed after the assay and measured in a scintillation counter.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Optical Measuring Cells (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

Pièces à insérer dans les cupules de plaques de microtitrage (2). Elles se composent d'un élément tubulaire creux à extrémités ouvertes, dont la surface interne et/ou externe se présente sous forme de zigzag ou de paroi cannelée (6) de manière à élargir la surface de la paroi enduite de réactif. La présente invention décrit en outre plusieurs versions de ladite pièce à insérer dans les cupules, entre autres une pièce de forme hélicoïdale et une pièce tubulaire à grandes mailles rectangulaires.
PCT/GB1990/001646 1989-10-27 1990-10-26 Pieces a inserer dans les cupules de plaques de microtitrage Ceased WO1991006368A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB8924252A GB2239947A (en) 1989-10-27 1989-10-27 Microtitre plate well inserts
GB8924252.3 1989-10-27

Publications (1)

Publication Number Publication Date
WO1991006368A1 true WO1991006368A1 (fr) 1991-05-16

Family

ID=10665296

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1990/001646 Ceased WO1991006368A1 (fr) 1989-10-27 1990-10-26 Pieces a inserer dans les cupules de plaques de microtitrage

Country Status (2)

Country Link
GB (1) GB2239947A (fr)
WO (1) WO1991006368A1 (fr)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19506802A1 (de) * 1995-02-27 1996-08-29 Karl Reichart Reagensträger zur Zugabe von Reagentien
US6027695A (en) * 1998-04-01 2000-02-22 Dupont Pharmaceuticals Company Apparatus for holding small volumes of liquids
US6800491B2 (en) 2001-06-08 2004-10-05 Nalge Nunc International Corporation Robotic reservoir without liquid hangup
WO2011026559A1 (fr) * 2009-09-05 2011-03-10 Lonza Biologics Plc Système de plaque de puits profonds à couvercle
WO2012120270A1 (fr) * 2011-03-04 2012-09-13 University College Cardiff Consultants Limited Microplaque destinée à la microscopie corrélative
CN104520690A (zh) * 2012-05-25 2015-04-15 珠海恺瑞生物科技有限公司(中国) 用于进行结合测定的设备和方法
WO2017161707A1 (fr) * 2016-03-24 2017-09-28 锦州医科大学附属第一医院 Plaque de micro-titration
KR20200135639A (ko) * 2019-05-23 2020-12-03 원광대학교산학협력단 웰 플레이트 및 시편 홀더

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2248790T3 (es) * 2003-06-04 2006-03-16 Millipore Corporation Placa de filtracion universal de multiples pocillos.
US8182766B2 (en) 2004-05-04 2012-05-22 Emd Millipore Corporation Universal filter plate
GB2472384A (en) * 2009-07-31 2011-02-09 Simon Stafford Modified microplate
GB2493763A (en) * 2011-08-18 2013-02-20 Univ Cranfield Microplates with Enhanced Immobilisation capabilities
GB201209944D0 (en) * 2012-06-02 2012-07-18 Univ Cranfield Microplates with enhanced capabilities controlled by magnetic field
CN112533702A (zh) * 2018-07-19 2021-03-19 丹耐克斯技术有限公司 多重样品板

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2377629A1 (fr) * 1977-01-14 1978-08-11 Suovaniemi Finnpipette Piece moulee pour appareils utilises pour les dosages immunologiques et les reactions enzymatiques
FR2418457A1 (fr) * 1978-02-22 1979-09-21 Commissariat Energie Atomique Dispositif pour analyser une solution
EP0197729A2 (fr) * 1985-04-01 1986-10-15 Donald Patrick Kelly Procédé d'essai immunologique enzymatique
EP0296415A2 (fr) * 1987-06-19 1988-12-28 Pall Corporation Plaque à plusieurs trous contenant des insertions de membrane
US4795562A (en) * 1985-09-13 1989-01-03 Walsh James W Membrane batch-processing apparatus

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2147698B (en) * 1981-11-17 1986-05-08 Unilever Plc Test apparatus for immunoassay
EP0087899B1 (fr) * 1982-03-03 1986-12-30 Becton Dickinson and Company Dispositif d'examen à récipients multiples pour essais immunologiques

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2377629A1 (fr) * 1977-01-14 1978-08-11 Suovaniemi Finnpipette Piece moulee pour appareils utilises pour les dosages immunologiques et les reactions enzymatiques
FR2418457A1 (fr) * 1978-02-22 1979-09-21 Commissariat Energie Atomique Dispositif pour analyser une solution
EP0197729A2 (fr) * 1985-04-01 1986-10-15 Donald Patrick Kelly Procédé d'essai immunologique enzymatique
US4795562A (en) * 1985-09-13 1989-01-03 Walsh James W Membrane batch-processing apparatus
EP0296415A2 (fr) * 1987-06-19 1988-12-28 Pall Corporation Plaque à plusieurs trous contenant des insertions de membrane

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19506802A1 (de) * 1995-02-27 1996-08-29 Karl Reichart Reagensträger zur Zugabe von Reagentien
US6027695A (en) * 1998-04-01 2000-02-22 Dupont Pharmaceuticals Company Apparatus for holding small volumes of liquids
US6800491B2 (en) 2001-06-08 2004-10-05 Nalge Nunc International Corporation Robotic reservoir without liquid hangup
WO2011026559A1 (fr) * 2009-09-05 2011-03-10 Lonza Biologics Plc Système de plaque de puits profonds à couvercle
CN102481572A (zh) * 2009-09-05 2012-05-30 英国龙沙生物医药股份有限公司 有罩的深孔板系统
US9783770B2 (en) 2009-09-05 2017-10-10 Lonza Biologics Plc Deepwell plate system with lid
GB2502902A (en) * 2011-03-04 2013-12-11 Univ Cardiff Microplate for correlative microscopy
WO2012120270A1 (fr) * 2011-03-04 2012-09-13 University College Cardiff Consultants Limited Microplaque destinée à la microscopie corrélative
CN104520690A (zh) * 2012-05-25 2015-04-15 珠海恺瑞生物科技有限公司(中国) 用于进行结合测定的设备和方法
EP2856104A4 (fr) * 2012-05-25 2016-02-24 Ronghao Li Appareils et procédés permettant de procéder à des dosages de liaison
WO2017161707A1 (fr) * 2016-03-24 2017-09-28 锦州医科大学附属第一医院 Plaque de micro-titration
KR20200135639A (ko) * 2019-05-23 2020-12-03 원광대학교산학협력단 웰 플레이트 및 시편 홀더
KR102252421B1 (ko) * 2019-05-23 2021-05-17 원광대학교산학협력단 웰 플레이트 및 시편 홀더

Also Published As

Publication number Publication date
GB8924252D0 (en) 1989-12-13
GB2239947A (en) 1991-07-17

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