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WO1990006044A3 - PROCEDE DE PRODUCTION D'ADNc - Google Patents

PROCEDE DE PRODUCTION D'ADNc Download PDF

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Publication number
WO1990006044A3
WO1990006044A3 PCT/EP1989/001418 EP8901418W WO9006044A3 WO 1990006044 A3 WO1990006044 A3 WO 1990006044A3 EP 8901418 W EP8901418 W EP 8901418W WO 9006044 A3 WO9006044 A3 WO 9006044A3
Authority
WO
WIPO (PCT)
Prior art keywords
mrna
support
dna
insoluble support
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP1989/001418
Other languages
English (en)
Other versions
WO1990006044A2 (fr
Inventor
Erik Hornes
Lars Korsnes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies AS
Original Assignee
Dynal AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB888827158A external-priority patent/GB8827158D0/en
Priority claimed from GB888827159A external-priority patent/GB8827159D0/en
Application filed by Dynal AS filed Critical Dynal AS
Priority to EP90900183A priority Critical patent/EP0444119B1/fr
Priority to DE68922743T priority patent/DE68922743T2/de
Publication of WO1990006044A2 publication Critical patent/WO1990006044A2/fr
Publication of WO1990006044A3 publication Critical patent/WO1990006044A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Luminescent Compositions (AREA)
  • Saccharide Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

Le procédé comprend les étapes consistant: a) à mettre un liquide contenant de l'ARNm en contact avec un support insoluble auquel sont fixées des sondes d'ADN par leur terminaison 5' de sorte que l'ARNm est hybridé auxdites sondes et donc audit support; b) à éliminer ledit liquide; et c) à ajouter des enzymes et des nucléotides en solution de sorte que la sonde fonctionne comme amorce pour produire de l'ADNc sur les gabarits d'ARNm. La sonde d'ADN est oligo-dT ou une séquence d'ADN spécifique pouvant correspondre à une région conservée dans une classe de molécules d'ARNm. Le support insoluble peut se composer de particules magnétiques qui sont monodispersées et/ou supraparamagnétiques.
PCT/EP1989/001418 1988-11-21 1989-11-21 PROCEDE DE PRODUCTION D'ADNc Ceased WO1990006044A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP90900183A EP0444119B1 (fr) 1988-11-21 1989-11-21 PROCEDE DE PRODUCTION D'ADNc
DE68922743T DE68922743T2 (de) 1988-11-21 1989-11-21 Verfahren zur herstellung von cdns.

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB8827158.0 1988-11-21
GB888827158A GB8827158D0 (en) 1988-11-21 1988-11-21 Process for producing cdna
GB8827159.8 1988-11-21
GB888827159A GB8827159D0 (en) 1988-11-21 1988-11-21 Subtraction cdna library construction

Publications (2)

Publication Number Publication Date
WO1990006044A2 WO1990006044A2 (fr) 1990-06-14
WO1990006044A3 true WO1990006044A3 (fr) 1990-11-01

Family

ID=26294647

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1989/001418 Ceased WO1990006044A2 (fr) 1988-11-21 1989-11-21 PROCEDE DE PRODUCTION D'ADNc

Country Status (6)

Country Link
EP (1) EP0444119B1 (fr)
JP (1) JP2960776B2 (fr)
AT (1) ATE122721T1 (fr)
CA (1) CA2003500C (fr)
DE (1) DE68922743T2 (fr)
WO (1) WO1990006044A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7329496B2 (en) 1990-12-06 2008-02-12 Affymetrix, Inc. Sequencing of surface immobilized polymers utilizing microflourescence detection

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992018647A1 (fr) * 1991-04-15 1992-10-29 Sumitomo Electric Industries, Ltd. Procede de separation ou d'extraction d'acide nucleique a partir d'un echantillon contenant celui-ci
US6316182B1 (en) * 1992-01-15 2001-11-13 Nen Life Science Products, Inc. Detection of reverse transcriptase by DNA hybridization assay
JPH08500722A (ja) * 1992-01-29 1996-01-30 日立化成工業株式会社 ポリヌクレオチド固定化担体
US6300058B1 (en) 1992-01-29 2001-10-09 Hitachi Chemical Research Center, Inc. Method for measuring messenger RNA
US5580971A (en) * 1992-07-28 1996-12-03 Hitachi Chemical Company, Ltd. Fungal detection system based on rRNA probes
CA2140763A1 (fr) * 1992-07-28 1994-02-03 Masato Mitsuhashi Systeme de detection genique
US5591841A (en) * 1993-01-14 1997-01-07 Ji; Huamin Rapid purification of circular DNA by triplex-mediated affinity capture
US5610287A (en) * 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
US6100079A (en) * 1996-02-25 2000-08-08 Precision System Science Co., Ltd. Method for treating biopolymers, microorganisms or materials by using more than one type of magnetic particles
US5712127A (en) * 1996-04-29 1998-01-27 Genescape Inc. Subtractive amplification
EP0981535A4 (fr) * 1997-05-12 2000-11-29 Life Technologies Inc Procedes permettant de produire et de purifier les molecules d'acide nucleique
US6399334B1 (en) 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
US6083727A (en) * 1999-06-30 2000-07-04 Incyte Pharmaceuticals Inc. Methods and compositions for producing 5' enriched cDNA libraries
ATE329060T1 (de) * 2000-03-24 2006-06-15 Micromet Ag Mrna amplifizierung
JP4735926B2 (ja) * 2004-05-27 2011-07-27 アイシン精機株式会社 核酸の高効率回収方法
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US11732253B2 (en) 2020-02-03 2023-08-22 Tecan Genomics, Inc. Repurposing beads in sample cleanup
GB2621159B (en) * 2022-08-04 2025-08-13 Wobble Genomics Ltd Methods of preparing processed nucleic acid samples and detecting nucleic acids and devices therefor

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985004674A1 (fr) * 1984-04-05 1985-10-24 Life Technologies, Inc. Immobilisation d'acides nucleiques
EP0184056A2 (fr) * 1984-11-27 1986-06-11 Molecular Diagnostics, Inc. Production d'ADN à grande échelle
EP0223618A2 (fr) * 1985-07-18 1987-05-27 New York Medical College Procédé automatisable pour le séquençage des nucléotides
EP0265244A2 (fr) * 1986-10-23 1988-04-27 Amoco Corporation Procédés et appareil pour la sélection d'une cible et de son environnement dans les dosages d'affinité
WO1988007585A1 (fr) * 1987-04-02 1988-10-06 Genelabs, Incorporated Composition et procede de transcription a abondance egale
EP0288737A1 (fr) * 1987-04-01 1988-11-02 Miles Inc. Essai d'hybridation rapide utilisant une sonde immobilisée sur latex

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985004674A1 (fr) * 1984-04-05 1985-10-24 Life Technologies, Inc. Immobilisation d'acides nucleiques
EP0184056A2 (fr) * 1984-11-27 1986-06-11 Molecular Diagnostics, Inc. Production d'ADN à grande échelle
EP0223618A2 (fr) * 1985-07-18 1987-05-27 New York Medical College Procédé automatisable pour le séquençage des nucléotides
EP0265244A2 (fr) * 1986-10-23 1988-04-27 Amoco Corporation Procédés et appareil pour la sélection d'une cible et de son environnement dans les dosages d'affinité
EP0288737A1 (fr) * 1987-04-01 1988-11-02 Miles Inc. Essai d'hybridation rapide utilisant une sonde immobilisée sur latex
WO1988007585A1 (fr) * 1987-04-02 1988-10-06 Genelabs, Incorporated Composition et procede de transcription a abondance egale

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Cell, Volume 21, October 1980, MIT, C.R. ZIMMERMAN et al.: "Molecular Cloning and Selection of Genes Regulated in Aspergillus Development", pages 709-715 see the whole article *
Nature, Volume 308, 8 March 1984, S.M. HEDRICK et al.: "Isolation of cDNA Clones Encoding T Cell-Specific Membrane-Associated Proteins", pages 149-153 see the whole article *
Nucleic Acids Research, Volume 15, No. 13, 10 July 1987, IRL Press, (Oxford, GB), T.R. GINGERAS et al.: "Hybridization Properties of Immobilized Nucleic Acids", pages 5373-5389 see "Introduction; "Materials and Methods" *
Nucleic Acids Research, Volume 16, No. 13, 11 July 1988, IRL Press, (Oxford, GB), T. ATKINSON et al.: "A Conventient Procedure for the Synthesis of Oligodeoxyribonucleotide Affinity columns for the Isolation of mRNA", page 6232 see the whole article *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7329496B2 (en) 1990-12-06 2008-02-12 Affymetrix, Inc. Sequencing of surface immobilized polymers utilizing microflourescence detection
US7459275B2 (en) 1990-12-06 2008-12-02 Affymetrix, Inc. Sequencing of surface immobilized polymers utilizing microfluorescence detection

Also Published As

Publication number Publication date
AU627815B2 (en) 1992-09-03
WO1990006044A2 (fr) 1990-06-14
EP0444119A1 (fr) 1991-09-04
CA2003500A1 (fr) 1990-05-21
ATE122721T1 (de) 1995-06-15
AU4758590A (en) 1990-06-26
DE68922743D1 (de) 1995-06-22
CA2003500C (fr) 2000-01-18
DE68922743T2 (de) 1995-10-26
EP0444119B1 (fr) 1995-05-17
JP2960776B2 (ja) 1999-10-12
JPH04501958A (ja) 1992-04-09

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