WO1990002167A1 - Kit for the reactivation of dehydrated microbial cultures, particularly of bacteria - Google Patents
Kit for the reactivation of dehydrated microbial cultures, particularly of bacteria Download PDFInfo
- Publication number
- WO1990002167A1 WO1990002167A1 PCT/EP1989/000930 EP8900930W WO9002167A1 WO 1990002167 A1 WO1990002167 A1 WO 1990002167A1 EP 8900930 W EP8900930 W EP 8900930W WO 9002167 A1 WO9002167 A1 WO 9002167A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- container
- kit
- kit according
- reactivation
- stirrer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/03—Means for pre-treatment of biological substances by control of the humidity or content of liquids; Drying
Definitions
- the present invention refers to a kit for the re-activation of dehydrated microbial cultures, particularly of bacteria-
- the object of the invention is to avoid the above said drawbacks by providing a kit for the reactivation of dehydrated microbial cultures, allowing the operations to be carried out in conditions of absolute sterility and the manteinance of a perfect Constance of the temperature.
- the kit according to the invention subtantially comprises a stiff container in plastic material, preferably polythene, with the corresponding cap, a thermosealed protective film, preferably of polythene for alimentary use, sterilized by gamma rays, an inertial thermostat in foamed material, for instance of foamed polystyrene, a stirrer in tear-proof plastic material, for instance of PVC, and optionally a thermometer and a pH measurement paper strip and an anhydrous dose of a specific microbial culture.
- the thermostat in foamed material preferably comprises an envelope containing the stiff conatiner and it is divided in two halves.
- kits are extremely simple and economic and it may be used by untrained personell.
- the reactivation of the microbial culture by means of the kit of the invention is carried out in the following way:
- the stiff container is inserted in one of the two halves of the thermostat in foamed material; - one of the two thermosealed edges of the sterile polythene bag is cut and the open bag is inserted in the stiff container with the opening towards the upper side;
- the bag is closed, for instance by means of a rubber band and the container by its cap;
- kits for the reactivation of microbial cultures according to the invention will be hereinafter described with reference to the annexed figure, showing in perspective view the different components.
- the kit according to the invention comprises a stiff container 1, preferably in polythene, substantially cylindrical, with the corresponding cap 2, an inertial thermostat in foamed polystyrene 3 , forming a covering envelope of the container 1 and divided in two identical halves 4 and 5, a rod stirred 6, a thermometer 7, a pH measuring paper strip 9 and a thermosealed protective film for alimentary use, sterilized by gamma rays, which is not shown in the enclosed figure.
- the stirrer consists in an holed washer having along its circumpherence a groove provided to contain an O-ring in soft material, acting as schock absorber, the washer itself being fixed into a rod having lenght so as to prevent the direct contact of the washer with the bottom of the container, so as to avoid the tearing of the polythene bag during stirring. .
- thermosealed edge of the sterile polythene bag (not shown) is cut and inserted into the stiff container 1 with the opening toward the upper side; - a certain amount of warm tap water (about 30 1 at a temperature of about 30-35°C) is introduced into the container 1;
- the anhydrous dose 9 is introduced as a powder into the container 1; - the mixture is stirred by the stirrer 6 up to complete dissolution of the powder;
- the correct pH value is checked by means of the pH measurement strip.
- the kit of the invention By means of the kit of the invention, allowing to work in conditions of absolute sterility and at perfectly constant temperature, even in environments where it is not normally possible to secure these conditions, excellent results are obtained in the reactivation process of microbial cultures; the microorganisms turn out to be much more active and numerically increased so as to allow working yields even ten times higher with the same dose of used microbial culture. It should be pointed out that the apparatuses comprising the kit are particularly cheap and easy to use also by untrained personell.
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A kit comprises a stiff container (1) in plastic material, preferably polythene, with the correspondig cap (2), a thermosealed protective film, sterilized by gamma rays, an inertial thermostat (3) in foamed material, a stirrer (6) in tear-proof plastic material, and optionally a thermometer (7) and a pH measurement paper strip (8) and an anhydrous dose of a specific microbial culture (9) is described.
Description
KIT FOR THE REACTIVATION OF DEHYDRATED MICROBIAL CULTURES, PARTICULARLY OF BACTERIA
The present invention refers to a kit for the re-activation of dehydrated microbial cultures, particularly of bacteria-
The reactivation of a bacterial culture which has been dehydrated for conservation purposes is necessary to allow the full metabolic functionality of the microorganisms and particularly to guarantee an high fermentative ability for the final substrates thereof.
In order to obtain satisfactory results. it is necessary that the preparation is carried out in conditions of absolute bacteriologic sterility and at constant temperature for a prefixed time, the better parameters being usually reported on the product packaging and changing acccording to formulations. Usually, the commercial anhydrous microbial cultures are reconεituted, i.e. brought again to the liquid status, either by simply suspending them in water or by directly adding them to the substrate which they must ferment. These operations are carried out in somewhat uncomfortable conditions by means of apparatuses not specifically designed for the intended use, with consequent difficulties in securing the above said conditions , with a consequent low yield in the use thereof.
The object of the invention is to avoid the above said drawbacks by providing a kit for the reactivation of
dehydrated microbial cultures, allowing the operations to be carried out in conditions of absolute sterility and the manteinance of a perfect Constance of the temperature. The kit according to the invention subtantially comprises a stiff container in plastic material, preferably polythene, with the corresponding cap, a thermosealed protective film, preferably of polythene for alimentary use, sterilized by gamma rays, an inertial thermostat in foamed material, for instance of foamed polystyrene, a stirrer in tear-proof plastic material, for instance of PVC, and optionally a thermometer and a pH measurement paper strip and an anhydrous dose of a specific microbial culture. The thermostat in foamed material preferably comprises an envelope containing the stiff conatiner and it is divided in two halves.
Said kit is extremely simple and economic and it may be used by untrained personell. The reactivation of the microbial culture by means of the kit of the invention is carried out in the following way:
- the stiff container is inserted in one of the two halves of the thermostat in foamed material; - one of the two thermosealed edges of the sterile polythene bag is cut and the open bag is inserted in the stiff container with the opening towards the upper side;
- about 30 liters of warm (about 30-35°C) tap water are introduced in the bag;
- the content of an anhydrous dose is poured therein;
- the mixture is stirred up to the complete dissolution of the powder;
- further tap water is added up to the container edge, checking the temperature by means of the thermometer;
- the bag is closed, for instance by means of a rubber band and the container by its cap;
- the other half of the thermostat in foamed material is placed so as to hold the container, keeping the kit in these conditions for the. specified time;
- the correct pH value is checked by means of the pH measurement paper strip.
A preferred embodiment of the kit for the reactivation of microbial cultures according to the invention will be hereinafter described with reference to the annexed figure, showing in perspective view the different components.
With reference to said figure, the kit according to the invention comprises a stiff container 1, preferably in polythene, substantially cylindrical, with the corresponding cap 2, an inertial thermostat in foamed polystyrene 3 , forming a covering envelope of the container 1 and divided in two identical halves 4 and 5, a rod stirred 6, a thermometer 7, a pH measuring paper strip 9 and a thermosealed protective film for alimentary use, sterilized by gamma rays, which is not shown in the enclosed figure.
According to a preferred embodiment, the stirrer consists in an holed washer having along its circumpherence a groove provided to contain an O-ring in
soft material, acting as schock absorber, the washer itself being fixed into a rod having lenght so as to prevent the direct contact of the washer with the bottom of the container, so as to avoid the tearing of the polythene bag during stirring. .
The reactivation of the microbial culture using the kit according to the invention is carried out in the following way:
- the stiff container 1 is inserted into one of the two halves of the thermostatic envelope 3;
- one of the two thermosealed edge of the sterile polythene bag (not shown) is cut and inserted into the stiff container 1 with the opening toward the upper side; - a certain amount of warm tap water (about 30 1 at a temperature of about 30-35°C) is introduced into the container 1;
- the anhydrous dose 9 is introduced as a powder into the container 1; - the mixture is stirred by the stirrer 6 up to complete dissolution of the powder;
- further tap water is added up to the container upper edge, checking the temperature of the mixture by the thermometer 7; — the sterile bag is closed, for instance by means of a rubber band, and the cap 2 is applied on the container l;
- the other half of the polystirene thermostatic envelope is inserted, and the whole kit is kept in these conditions for the time specified in the instructions
enclosed to the different kinds of cultures.
The correct pH value is checked by means of the pH measurement strip.
When the reactivation is ended, the product is ready to use.
By means of the kit of the invention, allowing to work in conditions of absolute sterility and at perfectly constant temperature, even in environments where it is not normally possible to secure these conditions, excellent results are obtained in the reactivation process of microbial cultures; the microorganisms turn out to be much more active and numerically increased so as to allow working yields even ten times higher with the same dose of used microbial culture. It should be pointed out that the apparatuses comprising the kit are particularly cheap and easy to use also by untrained personell.
Of course the invention is not limited to the particular embodiment above described and shown in the enclosed figure, and several changes may be made to it without departing from the scope of the invention.
Claims
1. A kit for the reactivation of dehydrated microbial culture, particularly of bacteria, comprising: - a stiff container 1 with the corresponding cap (2);
- a thermosealed protective film', sterilized by gamma rays, suited to coat the inner walls of the container (1) during the use;
- an envelope in foamed material (3) covering the container (1) and acting as an inertial thermostat;
- an anhydrous dose of a specific microbial culture (9) to be mixed with water in the container (1);
- a rod stirrer (6), and optionally a thermometer (7) and pH measuring paper strips (8).
2. A kit according to claim 1, characterized in that the thermostatic envelope (3) consists of two halves (4 and 5) .
3. A kit according to claim 1, characterized in that said container (1) is of cylindrical shape.
4. A kit according to any one of claims 1 to 3, characterized in that the container (1), the corresponding cap (2) and the protective film are made of polyethylene and the stirrer (6) is made of PVC.
5. A kit according to any one oc claims 1-4, characterized in that the envelope acting as inertial thermostat is made of foamed polystyrene.
6. A kit according to claims 1—5, characterized in that the stirrer consists of an holed washer having along its circumpherence a groove having an O-ring of soft material, said washer being fixed onto a rod having a lenght so as to avoid the direct contact of the washer with the bottom of the container.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT21731A/88 | 1988-08-23 | ||
| IT8821731A IT1226750B (en) | 1988-08-23 | 1988-08-23 | KIT FOR THE REACTIVATION OF DEHYDRATED MICROBIAL CULTURES, IN PARTICULAR OF BACTERIA. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO1990002167A1 true WO1990002167A1 (en) | 1990-03-08 |
Family
ID=11186066
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/EP1989/000930 Ceased WO1990002167A1 (en) | 1988-08-23 | 1989-08-07 | Kit for the reactivation of dehydrated microbial cultures, particularly of bacteria |
Country Status (3)
| Country | Link |
|---|---|
| AU (1) | AU3984389A (en) |
| IT (1) | IT1226750B (en) |
| WO (1) | WO1990002167A1 (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0085681B1 (en) * | 1981-03-20 | 1985-08-07 | A-Betong Ab/Sabema Material Ab | A concrete foot-structure for posts or a concrete-post plinth having a fracture mechanism cast therein; and a method for manufacturing such a foot structure or plinth |
| GB2305936A (en) * | 1995-10-06 | 1997-04-23 | Jonathan William Lewis | Bacterial culture vessel having a lid able to hold encapsulated culture supplements |
| FR2767141A1 (en) * | 1997-08-08 | 1999-02-12 | Sanofi Elf | DEVICE FOR THE CULTURE OF LACTIC BACTERIA |
| US5935843A (en) * | 1992-02-13 | 1999-08-10 | Osprey Biotechnics, Inc. | Method and apparatus for waste degradation |
| US6245555B1 (en) * | 1998-09-01 | 2001-06-12 | The Penn State Research Foundation | Method and apparatus for aseptic growth or processing of biomass |
| US6245552B1 (en) * | 1992-02-13 | 2001-06-12 | Osprey Biotechnics, Inc. | Method for waste degradation |
| GB2429525A (en) * | 2005-03-11 | 2007-02-28 | Colman Group Inc | Test strip dispenser and thermometer holder |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR1078976A (en) * | 1951-11-03 | 1954-11-24 | Method and apparatus for the fermentation of milk and the like | |
| FR1389044A (en) * | 1963-01-23 | 1965-02-12 | S B Penick & Company | Apparatus for fermentation |
| FR1601395A (en) * | 1968-12-27 | 1970-08-17 | Continuous yeast culture in liquid to be - seeded | |
| GB1299075A (en) * | 1969-03-25 | 1972-12-06 | Nat Res Dev | Sampling device |
| FR2296010A1 (en) * | 1974-12-29 | 1976-07-23 | Spofa Usines Pharma | DEVICE FOR THE CULTURE OF MICROORGANISMS |
| FR2563531A1 (en) * | 1984-04-30 | 1985-10-31 | Hickinbotham Winemakers | PROCESS AND APPARATUS FOR FERMENTATION |
| GB2202549A (en) * | 1987-03-20 | 1988-09-28 | Philip John Whitney | Foldable fermenter |
-
1988
- 1988-08-23 IT IT8821731A patent/IT1226750B/en active
-
1989
- 1989-08-07 AU AU39843/89A patent/AU3984389A/en not_active Abandoned
- 1989-08-07 WO PCT/EP1989/000930 patent/WO1990002167A1/en not_active Ceased
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR1078976A (en) * | 1951-11-03 | 1954-11-24 | Method and apparatus for the fermentation of milk and the like | |
| FR1389044A (en) * | 1963-01-23 | 1965-02-12 | S B Penick & Company | Apparatus for fermentation |
| FR1601395A (en) * | 1968-12-27 | 1970-08-17 | Continuous yeast culture in liquid to be - seeded | |
| GB1299075A (en) * | 1969-03-25 | 1972-12-06 | Nat Res Dev | Sampling device |
| FR2296010A1 (en) * | 1974-12-29 | 1976-07-23 | Spofa Usines Pharma | DEVICE FOR THE CULTURE OF MICROORGANISMS |
| FR2563531A1 (en) * | 1984-04-30 | 1985-10-31 | Hickinbotham Winemakers | PROCESS AND APPARATUS FOR FERMENTATION |
| GB2202549A (en) * | 1987-03-20 | 1988-09-28 | Philip John Whitney | Foldable fermenter |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0085681B1 (en) * | 1981-03-20 | 1985-08-07 | A-Betong Ab/Sabema Material Ab | A concrete foot-structure for posts or a concrete-post plinth having a fracture mechanism cast therein; and a method for manufacturing such a foot structure or plinth |
| US5935843A (en) * | 1992-02-13 | 1999-08-10 | Osprey Biotechnics, Inc. | Method and apparatus for waste degradation |
| US6245552B1 (en) * | 1992-02-13 | 2001-06-12 | Osprey Biotechnics, Inc. | Method for waste degradation |
| GB2305936A (en) * | 1995-10-06 | 1997-04-23 | Jonathan William Lewis | Bacterial culture vessel having a lid able to hold encapsulated culture supplements |
| GB2305936B (en) * | 1995-10-06 | 1997-09-03 | Jonathan William Lewis | Sterile, disposable culture vessels for the rapid growth of bacteria |
| FR2767141A1 (en) * | 1997-08-08 | 1999-02-12 | Sanofi Elf | DEVICE FOR THE CULTURE OF LACTIC BACTERIA |
| WO1999007825A1 (en) * | 1997-08-08 | 1999-02-18 | Sanofi Sante Nutrition Animale | Device for lactic bacteria culture |
| US6245555B1 (en) * | 1998-09-01 | 2001-06-12 | The Penn State Research Foundation | Method and apparatus for aseptic growth or processing of biomass |
| US6709862B2 (en) | 1998-09-01 | 2004-03-23 | The Penn State Research Foundation | Growing cells in a reservoir formed of a flexible sterile plastic liner |
| GB2429525A (en) * | 2005-03-11 | 2007-02-28 | Colman Group Inc | Test strip dispenser and thermometer holder |
| US7455451B2 (en) | 2005-03-11 | 2008-11-25 | The Colman Group, Inc. | Test strip dispenser and thermometer holder |
Also Published As
| Publication number | Publication date |
|---|---|
| IT8821731A0 (en) | 1988-08-23 |
| AU3984389A (en) | 1990-03-23 |
| IT1226750B (en) | 1991-02-05 |
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