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WO1986003841A1 - Procede immunologique de mesure d'analytes instables par l'utilisation d'anticorps a reaction croisee - Google Patents

Procede immunologique de mesure d'analytes instables par l'utilisation d'anticorps a reaction croisee Download PDF

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Publication number
WO1986003841A1
WO1986003841A1 PCT/US1985/002561 US8502561W WO8603841A1 WO 1986003841 A1 WO1986003841 A1 WO 1986003841A1 US 8502561 W US8502561 W US 8502561W WO 8603841 A1 WO8603841 A1 WO 8603841A1
Authority
WO
WIPO (PCT)
Prior art keywords
analyte
penicillin
test
fluid
interest
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1985/002561
Other languages
English (en)
Inventor
Vipin D. Shah
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
International Immunoassay Laboratories Inc
Original Assignee
International Immunoassay Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by International Immunoassay Laboratories Inc filed Critical International Immunoassay Laboratories Inc
Publication of WO1986003841A1 publication Critical patent/WO1986003841A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9446—Antibacterials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms

Definitions

  • B highly purified analyte conjugated to a high molecular weight substance, or.
  • C a highly purified fragment of the analyte of interest as such or as a conjugated molecule
  • beta-lactam ring of penicillin and other such antibiotics is very reactive.
  • the native penicillin may undergo changes, particularity through opening of the beta-lactam ring.
  • Such conversion depends upon the environmental conditions such as pH, temperature, presence of sugars, proteins etc. to which the antibiotic is exposed.
  • the degree of conversion may be different when it is injected into animals to treat infection, from when it is present in milk or from when it is present in fermentation broth.
  • the process of this invention can be practiced to measure a family of analytes which contain a common labile group which gets similarly altered in the sample environment and on conjugation. Such is the case with * the family of beta-lactam antibiotics.
  • reaction mixture is put on a Sephadex column G25 (K26/40 - Pharmacia) equilibrated with 0.15M NaCl in 0.01 M phosphate buffer (pH7.4). Elution is done with the same buffer at laboratory temperature. Fractions of 0.5 ml are recovered and tested by ultraviolet spectroscopy at 280 nm. The penicilloyl-BGG conjugate is thus entirely separated from the excess free penicillin. An alternative method is next described.
  • silanizing and succinylating is carried out according to known procedures.
  • silanizing is accomplished generally by treating the inorganic metal oxide material with an aqeous solution (e.g., about 10%) of the triethoxyaminopropylsilane at a pH of about 4.
  • succinylating is accomplished by treating the material with either an aqueous solution of succinic anhydride at a pH of about 6, or an aqueous solution of succinic acid and carbodiimide.
  • test data shows that Pen.RSA conjugate could be used instead of penicillin conjugation.
  • test results show the use of the sequential assay technique.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Dans un procédé d'essais immunologiques d'analytes instables, on utilise des anticorps à réaction croisée, la structure moléculaire de l'analyte étant modifiée pour devenir spécifique à l'anticorps. Le procédé peut être utilisé dans des essais immunologiques compétitifs, séquentiels, immunométriques à deux sites. Une application particulière concerne le dépistage qualitatif et quantitatif d'antibiotiques bêta-lactam dans des fluides, notamment le dépistage et la mesure de la penicilline.
PCT/US1985/002561 1984-12-20 1985-12-20 Procede immunologique de mesure d'analytes instables par l'utilisation d'anticorps a reaction croisee Ceased WO1986003841A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US68434184A 1984-12-20 1984-12-20
US684,341 1984-12-20

Publications (1)

Publication Number Publication Date
WO1986003841A1 true WO1986003841A1 (fr) 1986-07-03

Family

ID=24747658

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1985/002561 Ceased WO1986003841A1 (fr) 1984-12-20 1985-12-20 Procede immunologique de mesure d'analytes instables par l'utilisation d'anticorps a reaction croisee

Country Status (3)

Country Link
EP (1) EP0207979A4 (fr)
AU (1) AU5357886A (fr)
WO (1) WO1986003841A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0309299A1 (fr) * 1987-09-24 1989-03-29 Cambridge Life Sciences Plc Procédé pour la production des conjugaisons antigéniques protéine-haptène et leurs anticorps correspondants
EP0256551A3 (fr) * 1986-08-19 1990-03-14 AGRITECH SYSTEMS, Inc. Conjugènes haptène-macromolécule utiles dans des essais à haptène
US5273909A (en) * 1991-06-03 1993-12-28 Quantix Systems, L.P. Immunoassay for the detection of small aliphatic organic compounds

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4288538A (en) * 1979-01-31 1981-09-08 Baxter Travenol Laboratories, Inc. Test method and composition therefor
US4347312A (en) * 1980-03-20 1982-08-31 Research Triangle Institute Detection of antibiotics in milk
US4478934A (en) * 1981-07-15 1984-10-23 Yamasa Shoyu Kabushiki Kaisha Determination of adenosine by immunoassay involving acylation of the adenosine

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4288538A (en) * 1979-01-31 1981-09-08 Baxter Travenol Laboratories, Inc. Test method and composition therefor
US4347312A (en) * 1980-03-20 1982-08-31 Research Triangle Institute Detection of antibiotics in milk
US4478934A (en) * 1981-07-15 1984-10-23 Yamasa Shoyu Kabushiki Kaisha Determination of adenosine by immunoassay involving acylation of the adenosine

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
European Journal of Immunology Vol. 6, issued 1976, (Federal Republic of Germany), A.M. ISETTA et al., "Affinity and Specificity of Pencillin-Antibody Interaction Determined by an Enzyme (E. Coli beta- Galactosidase) Immunoasay", 737-742, See page 740 Section 3.5 and figure 6, and page 741 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0256551A3 (fr) * 1986-08-19 1990-03-14 AGRITECH SYSTEMS, Inc. Conjugènes haptène-macromolécule utiles dans des essais à haptène
US5166078A (en) * 1986-08-19 1992-11-24 Idexx Laboratories, Inc. Hapten-macromolecule conjugates useful in hapten assays
EP0309299A1 (fr) * 1987-09-24 1989-03-29 Cambridge Life Sciences Plc Procédé pour la production des conjugaisons antigéniques protéine-haptène et leurs anticorps correspondants
WO1989003040A1 (fr) * 1987-09-24 1989-04-06 Cambridge Life Sciences Plc Procede de production de conjugues proteine-haptene atigeniques, et anticorps leur correspondant
GB2210372A (en) * 1987-09-24 1989-06-07 Cambridge Life Sciences Method for the production of antigenic protein-hapten conjugates, and antibodies corresponding thereto
US5273909A (en) * 1991-06-03 1993-12-28 Quantix Systems, L.P. Immunoassay for the detection of small aliphatic organic compounds

Also Published As

Publication number Publication date
EP0207979A4 (fr) 1989-04-12
EP0207979A1 (fr) 1987-01-14
AU5357886A (en) 1986-07-22

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