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WO1983001111A1 - Appareil de mesure de la fluorescence - Google Patents

Appareil de mesure de la fluorescence Download PDF

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Publication number
WO1983001111A1
WO1983001111A1 PCT/FI1982/000036 FI8200036W WO8301111A1 WO 1983001111 A1 WO1983001111 A1 WO 1983001111A1 FI 8200036 W FI8200036 W FI 8200036W WO 8301111 A1 WO8301111 A1 WO 8301111A1
Authority
WO
WIPO (PCT)
Prior art keywords
measurement
monochromator
detector
light
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FI1982/000036
Other languages
English (en)
Inventor
Oy Eflab
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of WO1983001111A1 publication Critical patent/WO1983001111A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6473—In-line geometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/04—Batch operation; multisample devices
    • G01N2201/0446—Multicell plate, sequential

Definitions

  • the subject of the present invention is an apparatus for the measurement of fluorescence out of a liquid sample, the said apparatus comprising a source of light, from which the light of measurement is arranged so as to be passed vertically through optics, a monochromator, and through the sample placed in a pit plate to a measurement monochromator and a detector.
  • the samples have been dosed into small cuvettes, which may also be arranged in the form of a line or plate-
  • the measurement takes place in the longitudinal axial direction of the cuvette so that the cuvette is placed in the apparatus in the space between the source of light required and the detector.
  • a monochromatic excitation light and a detector measuring the fluorescence radi ⁇ ation emitted from the sample are required.
  • a monochromatic excitation light and a detector measuring the fluorescence radi ⁇ ation emitted from the sample are required.
  • a monochromator such as an interference filter or a grid. Since fluorescence radiation is emitted uniformly in all directions, in principle it can be measured from any direction whatsoever. A factor disturbing the measurement is the strong excitation light, wherefore attempts have been made to minimize its effect on the measurement result by means of various arrangements.
  • the commonest mode of measurement in use is the so-called 90° measurement geometry.
  • the directions of the excitation light and of the measure- ment are at an angle of 90 in relation to each other. In this way the access of direct excitation light to the detector is eliminated.
  • the procedure in itself is simple, but it imposes considerable limitations on the geometry of the object to be measured.
  • the sample cuvettes can be treated only one by one or in the line form.
  • It is also possible to measure the fluorescence radiation emitted by the surface by means of a particular arrangement. In this case, the direc ⁇ tions of excitation and measurement form a rather small ( ⁇ 10 to 20°) angle.
  • Such a measurement geometry is usually used when the sample absorbs either the exci- tation radiation or the emitted fluorescence radiation strongly.
  • the apparatus for the measurement of fluore ⁇ scence in accordance with the invention is mainly char ⁇ acterized in that, for each process of measurement, the source of light, the optics, the monochromator, the sample to be measured, the measurement monochromator, and the detector are arranged so that they can be brought onto the same vertical straight line in relation to each other, in this sequence.
  • the invention comes out more closely from the following description and from the attached draw ⁇ ings, wherein
  • Figure 1 is a schematical side view of the apparatus for the measurement of fluorescence and Figure 2 shows a section at A-A in Fig. 1.
  • the source 1 of light and the detector 6 are placed at opposite sides of the sample to be measured (Fig. 1) .
  • the monochromator 5 in front of the detector 6 has been selected so that it is penetrable by the desired measurement wave-length ⁇ i only, but not by the excitation radiation ⁇ o producing the fluorescence.
  • Such a measurement geometry is highly advantageous when such samples are. supposed to be measured as are dosed in a so-called pit plate 4.
  • the most important requirement regarding the source 1 of light is a sufficient light intensity at the wave-length ⁇ o. Suitable sources of light are xenon or mercury gas-discharge lamps. Laser may also be used in some applications. In such a case, no monochromator 3 is required. As a detector 6, it is favourable to use a photomultiplier tube. As monochromators 3 and 5, it is possible to use interference filters or grids. The monochromator 3 and/or the measurement monochromator 5 are preferably exchangeable or removable out of the line of measurement.

Landscapes

  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Appareil de mesure de la fluorescence émise par un échantillon liquide, cet appareil comprenant une source de lumière (1) à partir de laquelle on fait passer la lumière de mesure verticalement au travers d'un système optique (2), d'un monochromateur (3) et au travers de l'échantillon placé dans un plateau à cuvette (4) puis le rayon lumineux de mesure arrive sur un monochromateur de mesure (5) et un détecteur (6). Selon l'invention, pour chaque processus de mesure, la source lumineuse (1), le système optique (2), le monochromateur (3), l'échantillon à mesurer, le monochromateur de mesure (5) et le détecteur (6) peuvent être amenés sur la même ligne droite verticale les uns par rapport aux autres et dans cette séquence.
PCT/FI1982/000036 1981-09-21 1982-09-20 Appareil de mesure de la fluorescence Ceased WO1983001111A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI812933A FI812933L (fi) 1981-09-21 1981-09-21 Anordning foer maetning av fluorescens
FI812933810921 1981-09-21

Publications (1)

Publication Number Publication Date
WO1983001111A1 true WO1983001111A1 (fr) 1983-03-31

Family

ID=8514714

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FI1982/000036 Ceased WO1983001111A1 (fr) 1981-09-21 1982-09-20 Appareil de mesure de la fluorescence

Country Status (4)

Country Link
EP (1) EP0088781A1 (fr)
JP (1) JPS58501735A (fr)
FI (1) FI812933L (fr)
WO (1) WO1983001111A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0108524A1 (fr) * 1982-10-12 1984-05-16 Dynatech Laboratories, Incorporated Fluoromètre
EP0135303B1 (fr) * 1983-08-24 1988-11-02 Labsystems Oy Cuvettes
US4810096A (en) * 1986-05-09 1989-03-07 Cambridge Life Sciences, Plc Plate reader

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE364367B (fr) * 1967-05-12 1974-02-18 Rec Scient Centre Nat
DE2451769A1 (de) * 1973-11-14 1975-05-15 Osmo Antero Suovaniemi Verfahren und vorrichtung zum automatischen ablesen und bestimmen von reaktionsergebnissen
DE2104393B2 (de) * 1971-01-30 1976-01-02 Original Hanau Quarzlampen Gmbh, 6450 Hanau Fluoreszenz-Analysen-Vorrichtung
SE383207B (sv) * 1972-08-22 1976-03-01 Atomic Energy Commission Kombinerad dynamisk fotometer och fluormeter av flerstationstyp.
FR2408136A1 (fr) * 1977-11-04 1979-06-01 Colin Bruno Appareil de mesure de densite optique
GB2014300A (en) * 1977-07-29 1979-08-22 Opto Electronic Displays Ltd Opacity measurement apparatus and method
DE2818000A1 (de) * 1978-04-25 1979-11-08 Gunnar Oosterloo Geraet zur vergleichenden fluoreszenzspektroskopie fuer den chemieunterricht
FR2430610A1 (fr) * 1978-07-07 1980-02-01 Pasteur Institut Procede et dispositif pour determiner la sensibilite de bacteries et levures aux produits antibiotiques
EP0012698A1 (fr) * 1978-12-18 1980-06-25 Merck & Co. Inc. Procédé et appareil automatisés pour la lecture de plaques d'essais virologiques
WO1982000359A1 (fr) * 1980-07-24 1982-02-04 Oy Labsystems Ensemble de cuvettes
WO1982000358A1 (fr) * 1980-07-24 1982-02-04 Oy Labsystems Procede de mesure, et cuvette
EP0046430A1 (fr) * 1980-08-14 1982-02-24 Commissariat à l'Energie Atomique Procédé pour la détection et la quantification d'agglutinats en temps réel

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE364367B (fr) * 1967-05-12 1974-02-18 Rec Scient Centre Nat
DE2104393B2 (de) * 1971-01-30 1976-01-02 Original Hanau Quarzlampen Gmbh, 6450 Hanau Fluoreszenz-Analysen-Vorrichtung
SE383207B (sv) * 1972-08-22 1976-03-01 Atomic Energy Commission Kombinerad dynamisk fotometer och fluormeter av flerstationstyp.
DE2451769A1 (de) * 1973-11-14 1975-05-15 Osmo Antero Suovaniemi Verfahren und vorrichtung zum automatischen ablesen und bestimmen von reaktionsergebnissen
GB2014300A (en) * 1977-07-29 1979-08-22 Opto Electronic Displays Ltd Opacity measurement apparatus and method
FR2408136A1 (fr) * 1977-11-04 1979-06-01 Colin Bruno Appareil de mesure de densite optique
DE2818000A1 (de) * 1978-04-25 1979-11-08 Gunnar Oosterloo Geraet zur vergleichenden fluoreszenzspektroskopie fuer den chemieunterricht
FR2430610A1 (fr) * 1978-07-07 1980-02-01 Pasteur Institut Procede et dispositif pour determiner la sensibilite de bacteries et levures aux produits antibiotiques
EP0012698A1 (fr) * 1978-12-18 1980-06-25 Merck & Co. Inc. Procédé et appareil automatisés pour la lecture de plaques d'essais virologiques
WO1982000359A1 (fr) * 1980-07-24 1982-02-04 Oy Labsystems Ensemble de cuvettes
WO1982000358A1 (fr) * 1980-07-24 1982-02-04 Oy Labsystems Procede de mesure, et cuvette
EP0046430A1 (fr) * 1980-08-14 1982-02-24 Commissariat à l'Energie Atomique Procédé pour la détection et la quantification d'agglutinats en temps réel

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0108524A1 (fr) * 1982-10-12 1984-05-16 Dynatech Laboratories, Incorporated Fluoromètre
EP0135303B1 (fr) * 1983-08-24 1988-11-02 Labsystems Oy Cuvettes
US4810096A (en) * 1986-05-09 1989-03-07 Cambridge Life Sciences, Plc Plate reader

Also Published As

Publication number Publication date
FI812933A7 (fi) 1983-03-22
EP0088781A1 (fr) 1983-09-21
FI812933L (fi) 1983-03-22
JPS58501735A (ja) 1983-10-13

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