USRE16162E - aiexandes - Google Patents

Description

Sept. 8, 1 925.

E. R. ALEXANDER APPARATUS FOR TREATING SERUM Ofiginal Filed Feb, 17,

' INVENTOR Eli/claw) ATTORNEY .tion, Serial E le, 224N335, filed December Reissued Sept. 8 1925s E'LLKGT ALEXANDER, D3? KAE'SAS $27333 fiYElPiiBATUS ETJK ERMJEKN 214 Original-"Ho. 1,539,1(352, late-fl may 26, 19535,

I); all whom it may concern:

"fBe it known that I,ELL1OT R. ALEXANDER? aeitizen of the United States, residing at Kansas City, in the county of Jackson and State of Missouri, have invented ocrtaiunew and useful Improvements in Apparatus for Treating Serum; and 1 (lo LlQClfilQ the fol lowing to be a full, clear, and exact clescription oi the invention, such as will enable others skilled in the art to which it upper-- tains to make and use the some, reference being bad to the accompanying drawings, and to the figures of reference marked thereon, Which form a part of this specificstion.

This invention relates to apparatus for preparing serum incl particularly for ti e treatment oi antiliog cholera serum in. which the serum rendered most potent for the purpose intended in on eflioieirt url expeditious mu'nnor, the present an;

being a division oi my O-Ul51lillllg opu It has generally been the pi'uotice to pro luce serum by procuring heulthy, immune ogs 21ml hyperimmunizing them by injceting virulent blood into their ear vein, the end of about ten (lays these hogs are bled and after the first lGlGSi iug, the bleeding is repealed at intervals of about serum. This crude blood is then. reliiieil by placing it in containers carried by powerful centrifw gal machines lCYOiVlIllg at oruie suiiioienlz to throw down all the blood corpusclos, bum teria and solid inert material, leaving only the clear, pure, refined serum. The um should then be sterilized m5 2iv tempermure sullicient to kill all germs or haul as Well as to coagulate or solidify the soi )le fibrin. It is then subjected to a second sen i'lfu'ging operation so as to brecipitute fibrin. leaving a clear ember colored liquid. it is then bottl d and tested further for sterility and potency. The Bureau of Animal 1nclustry of the United States now recognizes that a clear serum is very much more concentrated and stronger in potency than blood serum and as a result of investigation, the Department of Agriculture has madeit mandatory that blood serum, that is, serum which has not been defibrinetedymust be used in considerably larger doses than clear serum. The potency of clear serum, bowever can be rendered quite inefficient by All eriafl 1% $153,539 1' reissue filled July 2Q, 2825. Seriil improper treatment method. of deli Tlllflllli of high power cent par tion of the serum ,i unnecessarily merit i'uvoli/el phi lzainers the centr steriliuiugg it in the con suit "that the machine stood period of: sierilizutiou In. view fact [I mau ines speeais go "outed Gi lei? J ri. i,

must be cons? tonal see loroze in great 9 tb oeoo Aocorliiig placed in may be re is then i )ou i the co: miner turers of the o y islzeu t snugl host 1 trainers s thst on "re the receptacle or member is speceu from the outer receptacle sufficiently to prevent adhesion under high centrifugal pressure and the Wall of he outer receptacle is sul ficiently close to prevent permanent Iii" tion of the inner receptacle when iiie s? subjeui-ed to such high centrifugal pr" The product is again su .cl'ecl to of ihe centrifugal ins:

cipitution of the fibrin whereupon the clear serum may be bottled, ready for use.

According to my invent-ion, the blood serum may he introduced into the receptacle and the receptacle sealed, it l'ieing unnecessar to open it or pour out the contents una'l ter the sterilization takes place, this being an important step in advance oi known methods since the serum need not be subjected to contamination between the time that the hog is bled and the time the finished product bottled.

The receptacle and container constitute substantially double Walled receptacle, the inner all of which is relatively thin to permit in sterilization, and the outer Wall of which is relatively thick to resist the tendency to warp or disrupt under the action of the centrifugal force when the mucliiiie is operating, it being importunt that the two Walls lie close together so that therewill be no opportunity for the inner wall to bulge or otherwise distort.

From the foregoing it will be apparent that according to the method described, the liability of contamination. during the luiu-- dling oi the serum will be eliminated and it Will also be apparent that the method may be concluded in less time than ordinarily employed for a similar result and that the machine may he in practically continuous operation since a. number of rccepi soles our be provided for each container so that when defibrinzrting of the serum in one set of receptacles has been finished, the receptacles may be removed and euother set introduced, permitting the machine to produce the deiibriuirtion in one set oi receptacles While. the serum in the other set is being sterilized and since the recepteclesmey he provided with covers, the blood contained therein waiting to be conveyed. to the centrifuging machine will be sealed from contamination from an exterior source.

As illustrative of a mechanism for currying out the method, 1 have shown a. known form of centrifuging machine sufiiciently modified to perform the functions intended.

In. the drawings,

Fig. 1 is side elevationel View of centrifuging machine constructed n accordance with my invention.

a detail view of one or the con taiuers, sho 'ing the receptacle or inner ii 11-- inc; ertielly withdrawn, and

Fig. is 1?. vertical, long-itudinel sectional "view through the double walled container.

The centrifuging muchir is illustrated 2.: consisting of a. shaft 1 driven by bolt 2 from a. suitable source of power by passing uroundthe pulle 3. The shaft '1 is in been ings land 5 and at its upper end it is proided with radial arms 6 having hooked c;- ticiuities "4" to receive the truniuions 8 of the outer wali or container 9. The container l, is provided with relatively thick walls withstand the centrifugal lore. and serving as reinforcing member {or t o0 inner wall or receptacle 10 which contains the blood serum. to be trezatcrh the receptacle LU being provided with a lid or cover 11.

.l't will be apparent that when the parts are assembled as shown in Fig, l and the centril. ing machine is operating, the memhers 9 will assume substantially lmrizontul positions due to the centrifugal force gem eluted and. that (lviihiinuting will who piece. After it suitable time the inner wall or receptucle 10 may he removed and conveyed to ustcrilizer. substitute rccepi'zucles being introduced into the members 9 so that a substantially continuous operation may he meintaincd.

Nhst I claim and desire to secure by Letters Patent is 1.. A double Walled centrifugal separator container, the outer wall of which consists otun outerreceptacle lu1.viug substantially thick walls, and the inner wall of which 7 consists of a receptacle having substantially thin wells adapted to bulge under excessive pressure, the inner receptacle conforming suhstuutially throughout to the inner surface of the outer receptacle with its outer surface in such proximity to the inner sur' face of the outer receptucle us to prevent peruuuuuu' distortion. of the inner receptacle. there being suf'iicient space between the inner and outer walls to prevent :ulhcsion under high centrifugal pressure. i

A. double walled ccutrit'ugul sepurutm contsiuer, the outer wall of which consists of a substantially cylindriel outer inen'ihcr having relatively thick walls and provided with opposite trulini'ons for pirotally 'mouutiug the container'in centrifugal inucliine and the inner wall; of the said container consisting of areceptucle having substentiolly thin Walls adapted to bulge under excessive pressure, the inner recerltecie conforming substantially throughout to the cylindrical outer member with its outer surface in such proximity to the inner surface of the hollow member as to prevent per mu ncnt distortion of the inner rccel'itucle, iI-llflil being sufficient space hetweerrtho inner and outer walls to prevent adhesion under high centrifugal pressure.

In 'tcstimon whereof I my signature.

*JLLIGT R. ALEXANDER.

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