US20250089723A1 - Biocontrol agent and method for reducing the occurrence and impacts of aphanomyces euteiches - Google Patents
Biocontrol agent and method for reducing the occurrence and impacts of aphanomyces euteiches Download PDFInfo
- Publication number
- US20250089723A1 US20250089723A1 US18/291,944 US202218291944A US2025089723A1 US 20250089723 A1 US20250089723 A1 US 20250089723A1 US 202218291944 A US202218291944 A US 202218291944A US 2025089723 A1 US2025089723 A1 US 2025089723A1
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- plant
- euteiches
- griseoviridis
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- present disclosure
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/28—Streptomyces
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
Definitions
- the present disclosure relates to a biological control agent for reducing the occurrence and impacts or for controlling root rot of plants such as root rot caused by the fungus Aphanomyces euteiches.
- Legumes are very important to agriculture as they fix nitrogen in association with rhizobia, thereby reducing the need for fertilizer inputs. They are valued worldwide as a sustainable and inexpensive meat alternative and are considered the second most important food source after cereals. However, many pathogens cause significant yield losses in legumes. Soilborne oomycete pathogens attack roots of legumes, limit nutrient uptake and cause the damping-off and/or root rot disease complex which results in plant death.
- Aphanomyces root rot is one of the major limitations to several legume production, such as pea, lentil, fava bean and alfalfa production worldwide with very few means of treatments. It can infect the host plant at any time throughout the growing season. This disease is caused by Aphanomyces euteiches Drechs. ( A. euteiches ), which is distinguished from most other soil-borne pathogens by the formation of thick-walled oospores. These oospores are able to remain dormant for several years, even without the presence of the host. It has been reported in most legume, such as lentil and pea growing areas of North America, northern Europe, Australia, New Zealand and Japan. It can cause severe root damage, wilting, and considerable yield losses under wet soil conditions.
- the present disclosure relates to the use of microorganisms of the species Streptomyces griseoviridis which have shown to display antagonistic effects against A. euteiches.
- the present disclosure relates to a method or a use for protecting a plant from a pathogen Aphanomyces euteiches or for inhibiting the development of the pathogen A. euteiches comprising the steps of contacting the pathogen, the plant or plant part during a stage of the growth of said plant, the soil surrounding the plant or a seed of said plant with a strain of Streptomyces griseoviridis which has suppressive activity against A. euteiches .
- the present disclosure further provides a use of S. griseoviridis for protecting a plant from Aphanomyces euteiches or for inhibiting the development of A. euteiches.
- the strain of S. griseoviridis is the S. griseoviridis strain K61 (deposited under accession number DSM 7206).
- the plant is a legume plant such as pea, lentil, fava bean or alfalfa and the concentration of said S. griseoviridis is between about 10 2 to about 10 11 CFU/g or ml of carrier.
- FIG. 1 illustrates PDA plates after 51-54 hours of incubation with A. euteiches (small circles). Biological and chemical agents (in the center) were added to the plates 48 hours prior to introducing A. euteiches.
- the present disclosure provides a biological control agent and method for inhibiting the development of A. euteiches or for controlling Aphanomyces root rot in plants.
- the present disclosure is particularly directed to Streptomyces griseoviridis that is effective in controlling the soil-borne oomycete A. euteiches in legume fields, such as lentils or peas. More particularly, it has been demonstrated that Streptomyces griseoviridis strain K61 exhibits pronounced antagonist effects against A. euteiches . Accordingly, Streptomyces griseoviridis strain K61 is herewith provided as a biocontrol agent suitable for use against Aphanomyces root rot in legume fields, such as lentils or pea.
- biological control is defined as control of a pathogen and/or insect and/or an acarid and/or a nematode by the use of a second organism.
- Known mechanisms of biological control include bacteria that control root rot by out-competing fungi for space or nutrients on the surface of the root.
- Biological control agent as used herein may also encompass microorganisms having a beneficial effect on plant health, growth, vigor, stress response or yield.
- Application routes include spray application soil application and seed treatment.
- inhibiting or ‘inhibit” the development of a fungal pathogen means the ability of the biological control agent to retard, stabilize, reverse, slow or delay progression of an infection by the fungal pathogen. In other words, these terms are used herein to mean reduce the growth and/or development of fungi compared to where inhibiting biocontrol agent is not present.
- the present disclosure provides an alternative for inhibiting the development or colonization, treating and controlling Aphanomyces root rot in legume fields, such as lentils or peas.
- the biological control agent used in the present disclosure is known as Streptomyces griseoviridis strain K61 (or also referred to as Streptomyces sp. K61) (which can be isolated from the product Mycostop®, Lallemand). More particularly, Streptomyces griseoviridis K61 was deposited on 8 Aug. 1992 according to the Budapest Treaty under accession number DSM 7206 at the
- the antagonistic activity or the suppressive activity of Streptomyces sp. K61 against A. euteiches may be determined using the dual-culture technique.
- the bacterial strain is streaked down the centers of plates comprising a medium which will support the growth of A. euteiches .
- a mycelial plug of A. euteiches from a culture grown on potato dextrose agar is placed on both sides of the streaked line of each dish containing the bacterial streaks.
- the plates are incubated according to a required time and temperature and the inhibitory effect of the bacterial strain is determined by measuring the inhibition zone of mycelial growth. The inhibitory effect is scored as positive where the A. euteiches growth stops on or before the bacterial streak line.
- Streptomyces griseoviridis strain K61 of the present disclosure is known and can be cultivated and caused to sporulate using methods well known in the art. Harvesting of spores is further conducted using also methods well known in the art. The spore material is then dried by a suitable process such as air drying, freeze drying or desiccation with a suitable desiccant and can be reformulated by addition of inert filler to provide a suitable number of spores per unit amount of product.
- a suitable process such as air drying, freeze drying or desiccation with a suitable desiccant and can be reformulated by addition of inert filler to provide a suitable number of spores per unit amount of product.
- the bacteria may be utilized in the form of cultures, such as a suspension in a whole broth culture, to prepare appropriate compositions for ground treatment, plant treatment, soil and/or growing media treatment, or seed treatment.
- compositions containing the bacteria as the sole active ingredient, or as a combination with one or more other active ingredients are prepared in known manner.
- the strain e.g. the spores
- the carrier used in the context of the present disclosure is an “agriculturally acceptable carrier” which includes any liquid or solid carrier to which the bacteria of the present disclosure can be added and that is not harmful to the bacteria of the present disclosure or the plants to which it is being applied.
- An agriculturally acceptable carrier is any liquid or solid that can be combined with the S. griseoviridis of the present disclosure and that assists in the application of the S.
- griseoviridis strain of the present disclosure to the soil so that the S. griseoviridis strain of the present disclosure grow and colonize the soil and roots of field peas.
- agriculturally acceptable carriers include talc, starch, sucrose, lactose, and other carbohydrates, polysaccharides, milk/skim milk, cellulose, water, a buffered solution, methylcellulose, clay, sand, peat, vermiculite, diatomaceous earth, a cereal grain flour meal, cotton meal, rice, or seeds, and liquid media.
- a carrier can also be a polymeric porous material, such as polyvinyl alcohol (PVA), polyethylene glycol (PEG), polyacrylic acid (PAA), poly(lactic acid) (PLA), poly(glycolic acid) (PGA), copolymer of poly(lactic-co-glycolic acid) (PLGA), poly(c-caprolactic acid) (PCLA), poly(f3-hydroxybutyric acid), poly(f3-hydroxyvaleric acid), polydioxanone, poly(ethylene oxide), poly(malic acid), poly(tartronic acid), polyphosphazene, polyethylene (PE), polystyrene (PS), agar (alginate) or other polysaccharides, gelatin, or combinations thereof.
- PVA polyvinyl alcohol
- PEG polyethylene glycol
- PAA polyacrylic acid
- PLA poly(lactic acid)
- PGA poly(glycolic acid)
- PCLA poly(f3-hydroxybutyric acid)
- PCLA poly(f3
- the carrier should not kill the bacteria of the present disclosure. Also, the carrier should release the bacteria of the present disclosure into the soil after application to the soil or onto plants after application onto the plants. Such release of the bacteria may occur after a period of rain or irrigation or other type of application of water to the soil and/or plant. Any carrier that permits the bacteria of the present disclosure to be delivered to the soil and/or target plant in a manner such that the bacteria remains viable may be employed in the composition so long as the carrier does not harm native plants and crops.
- the S. griseoviridis of the present disclosure can be combined with other biocontrol agents and applied sequentially or together. Further, the S. griseoviridis of the present disclosure may also be used in combination with biologically compatible pesticidal active agents as for example, herbicides, nematocides, fungicides, insecticides, and the like. They can also be used in combination with plant growth affecting substances, such as fertilizers, plant growth regulators, and the like, provided that such compounds or substances are biologically compatible.
- the active constituents are used in a concentration sufficient to inhibit targeted plant pathogen development when applied to the plants.
- typical concentrations are those higher than 1 ⁇ 10 2 CFU (“Colony Forming Unit”)/g or ml of carrier.
- Preferred concentrations range from about 1 ⁇ 10 4 to about 1 ⁇ 10 9 CFU/g or ml of carrier, such as the concentrations ranging from 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU/g or ml of carrier.
- the S. griseoviridis of the present disclosure may be applied to the plants using any known conventional methods such as dusting, injecting, rubbing, rolling, dipping, drenching, spraying, or brushing, or any other appropriate technique which does not injury the plants to be treated.
- seeds e.g. pea Pisum sativum L. seeds
- a preferred method for coating the seeds is to combine the bacterial strain with a biologically non-interfering liquid carrier for application onto the seeds.
- a carrier shall be deemed “biologically non-interfering” if it does not prevent the bacterial strains from growing.
- A. euteiches was subcultured onto potato dextrose agar (PDA) plates and incubated in the dark for 48-72 hours at 23° C. Streptomyces sp. K61 was isolated from MYCOSTOP®. The product was resuspended in water and streaked onto TSA plates followed by incubation at 28° C. for 48 hours.
- INTEGO® Solo is a seed-applied fungicide that contains the active ingredient ethaboxam (34.2% by weight) and is the only chemical treatment that is registered to suppress A. euteiches . Once all the cultures and materials were prepared, the following protocol was completed.
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Dentistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US18/291,944 US20250089723A1 (en) | 2021-08-04 | 2022-07-29 | Biocontrol agent and method for reducing the occurrence and impacts of aphanomyces euteiches |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163229228P | 2021-08-04 | 2021-08-04 | |
| US18/291,944 US20250089723A1 (en) | 2021-08-04 | 2022-07-29 | Biocontrol agent and method for reducing the occurrence and impacts of aphanomyces euteiches |
| PCT/IB2022/057093 WO2023012631A1 (fr) | 2021-08-04 | 2022-07-29 | Agent de lutte biologique et procédé pour réduire l'apparition et les impacts d'aphanomyces euteiches |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| US20250089723A1 true US20250089723A1 (en) | 2025-03-20 |
Family
ID=82932683
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US18/291,944 Pending US20250089723A1 (en) | 2021-08-04 | 2022-07-29 | Biocontrol agent and method for reducing the occurrence and impacts of aphanomyces euteiches |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20250089723A1 (fr) |
| EP (1) | EP4380370A1 (fr) |
| CA (1) | CA3228170A1 (fr) |
| WO (1) | WO2023012631A1 (fr) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160165889A1 (en) * | 2013-07-24 | 2016-06-16 | Bayer Cropscience Ag | Binary fungicidal composition |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5527526A (en) * | 1993-06-30 | 1996-06-18 | Idaho Research Foundation, Inc. | Use of streptomyces bacteria to control plant pathogens |
| US5968503A (en) * | 1993-06-30 | 1999-10-19 | Idaho Research Foundation, Inc. | Use of streptomyces bacteria to control plant pathogens and degrade turf thatch |
| CA3163901A1 (fr) * | 2019-12-05 | 2021-06-10 | Danstar Ferment Ag | Formulation comprenant des streptomyces spp. destinee a etre utilisee dans le traitement de semences |
-
2022
- 2022-07-29 WO PCT/IB2022/057093 patent/WO2023012631A1/fr not_active Ceased
- 2022-07-29 CA CA3228170A patent/CA3228170A1/fr active Pending
- 2022-07-29 EP EP22754953.2A patent/EP4380370A1/fr active Pending
- 2022-07-29 US US18/291,944 patent/US20250089723A1/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160165889A1 (en) * | 2013-07-24 | 2016-06-16 | Bayer Cropscience Ag | Binary fungicidal composition |
Also Published As
| Publication number | Publication date |
|---|---|
| EP4380370A1 (fr) | 2024-06-12 |
| WO2023012631A1 (fr) | 2023-02-09 |
| CA3228170A1 (fr) | 2023-02-09 |
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Owner name: LALLEMAND INC., CANADA Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:LALLEMAND SPECIALTIES INC.;REEL/FRAME:066259/0381 Effective date: 20230113 Owner name: DANSTAR FERMENT AG, SWITZERLAND Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:LALLEMAND INC.;REEL/FRAME:066259/0270 Effective date: 20230116 Owner name: LALLEMAND SPECIALTIES INC., WISCONSIN Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:KRAUSE, MATTHEW;REEL/FRAME:066259/0230 Effective date: 20221222 Owner name: DANSTAR FERMENT AG, SWITZERLAND Free format text: ASSIGNMENT OF ASSIGNOR'S INTEREST;ASSIGNOR:LALLEMAND INC.;REEL/FRAME:066259/0270 Effective date: 20230116 Owner name: LALLEMAND SPECIALTIES INC., WISCONSIN Free format text: ASSIGNMENT OF ASSIGNOR'S INTEREST;ASSIGNOR:KRAUSE, MATTHEW;REEL/FRAME:066259/0230 Effective date: 20221222 Owner name: LALLEMAND INC., CANADA Free format text: ASSIGNMENT OF ASSIGNOR'S INTEREST;ASSIGNOR:LALLEMAND SPECIALTIES INC.;REEL/FRAME:066259/0381 Effective date: 20230113 |
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