Classifications

• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
  • A01N65/08—Magnoliopsida [dicotyledons]
  • A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
  • A61K36/18—Magnoliophyta (angiosperms)
  • A61K36/185—Magnoliopsida (dicotyledons)
  • A61K36/75—Rutaceae (Rue family)
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/10—Antimycotics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P33/00—Antiparasitic agents
  • A61P33/10—Anthelmintics
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
  • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
  • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
  • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

• the present invention is related to how to obtain an extract from Swingles glutinosa leaves, and uses of the extract.
• Swingles glutinosa derived compounds had been described to have multiple beneficial uses.
• Weniger B. et al. discloses possible uses of acridone alkaloids from Swingles glutinosa against Plasmodium falciparum, a protozoan which is the main cause of malaria disease (see Weniger B. et al., Bireactive Acridone Alkaloids from Swinglea glutinosa, J. Nat. Prod., 2001, 64(9):1221-3).
• Braga P. A. C. et al. teaches the citotoxicity activity of derivatives of Swingles glutinosa against cancer cell lines (see Braga P. A. C. et al., In Vitro Citotoxicity Activity on Several Cancer Cell Lines of Acridone Alkaloids and N-Phenylethyl-Benzamide derivatives from Swingles glutinosa , Natural Product Research, 2007, 21(1):47-55).
• Bueno-Sanchez J. G. et al. describes the potential of essential oils from Swingles glutinosa against Mycobacterium tuberculosis, the tuberculosis causing agent (see Bueno-Sanchez J. G. et al., Assessment of Antimycobacterial Activity of Several Aromatic and Medicinal Plants that Grow in Colombia, Memoirs of VI National Encounter of Investigation in Infectious Diseases, Colombian Association of Infectology, 2008, page 96).
• the invention of the present Application provides a standardized method to obtain an extract and the extract from leaves of Swingles glutinosa , wherein the method yields an amount of extract that is about 60% the weight of the Swinglea glutinosa leaves.
• the present Application invention provides an extract isolated from Swingles glutinosa leaves, wherein said extract is obtained by a method comprising:
• the extract obtained by the method of the present Application invention, is used to kill fungi.
• the extract obtained by the method of the present Application invention, is used to kill insects and mites.
• the extract obtained by the method of the present Application invention, is used to repel insects and mites.
• the present Application invention provides a method for the production of an extract derived from Swingles glutinosa plant, wherein said method comprises:
• the present invention provides an extract isolated from Swingles glutinosa leaves, wherein said extract is obtained by a method comprising:
• the leaves are protected in any way possible from direct exposure to sunlight.
• the leaves that turn yellowish instead of opaque green are discarded.
• the period that the leaves take to turn into brittle leaves, and to turn from bright green leaves into opaque green leaves, depends on the optimal exposure to an air flow (aeration).
• aeration occurs at 25° C., although changes in temperature may change the aeration period, e.g., small increases in temperature may shorten the aeration period.
• the leaves must not be broken into leaf fragments that are less than 0.05 mm, since smaller fragments would tend to become a single mass which will become a limitation for the optimal extraction with a solvent.
• the leaf fragments are exposed to contact to the solvent by re-circulating the solvent throughout the bed where the leaf fragments are.
• the leaf fragments could be the ones moving throughout a container with solvent.
• the vaporized solvent is re-liquefied and reused for a new cycle. There are as many cycles as necessary, until when there is not more extract obtained to keep. According to the method of the present invention, this is achieved when the remaining weight of the fragments leaf mass left is approximately 40% with respect to the weight of the leaf fragments before the first exposure to the solvent.
• the extract obtained constitutes approximately 60% of the initial leaf fragments weight
• the solvent that can be used comprises ethanol, methanol, hexane, propanol, isopropanol, CO 2 , acetone, water, ethyl-acetate, nitrile-acetate, toluene, tetrahydrofurane, Chloroform, dichloromethane, and others.
• the extract obtained by the method of the present Application invention, is used to kill fungi.
• the extract obtained by the method of the present Application invention, is used to kill insects and mites.
• the extract obtained by the method of the present Application invention, is used to repel insects and mites.
• the present Application invention provides a method for the production of an extract derived from Swingles glutinosa plant, wherein said method comprises:
• the extract of can be used to kill fungi, wherein susceptible fungi comprises:
• the extract can be used to kill insects and mites, wherein susceptible insects and mites comprise:
• the extract can be used to repel insects and mites, wherein susceptible insects and mites comprise:
• the extract of the present invention can be combined with camphor and oils that have been described to kill fungi, kill and or repel insects and mites, wherein the oils would enhance the effects of the Swingles glutinosa extract against fungi, insects, and mites, and wherein the oils can be derived from garlic, orange, lemon, lime, Cymbopogon sp., Eugenia caryophyllata, Eucalyptus sp., Melaleuca alternifolia, Citrus simensis, other citrus sp., cinnamon, and others
• Branches with dark bright green leaves of Swinglea glutinosa tree were collected and put under a roof at approximately 25° C., in a place where there was a natural air flow.
• the branches with leaves were periodically moved to guarantee exposition all leaves to the air flow.
• the leaves that were dry and turned opaque green were collected from the branches, and said dry opaque green leaves were broken into fragments of no more than 0.05 mm with a blade mill.
• About 30 Kilograms of leaf fragments were put into a steel sieve with 0.05 mm net-apertures.
• the container holding the steel sieve holding the leaf fragments was filled with a mix of 320 liters ethanol and 70 liters of water for about 45 minutes.
• the ethanol-water mix was withdrawn and the ethanol-water was vaporized by heating. The remaining extract was kept, and the vaporized ethanol-water was turned into liquid again in a separate closed spaced with a condenser. The ethanol-water mix was reused to fill the container holding the steel sieve with leaf fragments for six more times, each time from 25 minutes to 35 minutes. The final amount of extract obtained was about 18 liters, wherein said extract was a dark green viscous material.

Landscapes

• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• General Health & Medical Sciences (AREA)
• Natural Medicines & Medicinal Plants (AREA)
• Engineering & Computer Science (AREA)
• Mycology (AREA)
• Microbiology (AREA)
• Biotechnology (AREA)
• Veterinary Medicine (AREA)
• Chemical & Material Sciences (AREA)
• Pharmacology & Pharmacy (AREA)
• Medicinal Chemistry (AREA)
• Animal Behavior & Ethology (AREA)
• Public Health (AREA)
• Wood Science & Technology (AREA)
• Environmental Sciences (AREA)
• Agronomy & Crop Science (AREA)
• Plant Pathology (AREA)
• Dentistry (AREA)
• Zoology (AREA)
• Botany (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• General Chemical & Material Sciences (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Organic Chemistry (AREA)
• Epidemiology (AREA)
• Alternative & Traditional Medicine (AREA)
• Medical Informatics (AREA)
• Communicable Diseases (AREA)
• Oncology (AREA)
• Tropical Medicine & Parasitology (AREA)
• Agricultural Chemicals And Associated Chemicals (AREA)
• Medicines Containing Plant Substances (AREA)
• Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
• Inorganic Chemistry (AREA)
• Pest Control & Pesticides (AREA)
• Peptides Or Proteins (AREA)
• Compounds Of Unknown Constitution (AREA)

Priority Applications (21)

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Related Child Applications (5)

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Citations (8)

• 2009
  • 2009-05-15 US US12/466,801 patent/US20100291241A1/en not_active Abandoned
• 2010
  • 2010-05-14 ES ES10774608T patent/ES2772049T3/es active Active
  • 2010-05-14 WO PCT/IB2010/001129 patent/WO2010131109A1/en not_active Ceased
  • 2010-05-14 CN CN2010800209591A patent/CN102791252A/zh active Pending
  • 2010-05-14 CA CA2761508A patent/CA2761508A1/en not_active Abandoned
  • 2010-05-14 MX MX2011012121A patent/MX2011012121A/es active IP Right Grant
  • 2010-05-14 EP EP10774608.3A patent/EP2429488B1/en active Active
  • 2010-05-14 JP JP2012510382A patent/JP2012526794A/ja active Pending
  • 2010-05-14 PE PE2011001943A patent/PE20120904A1/es not_active Application Discontinuation
  • 2010-05-14 AR ARP100101695A patent/AR076874A1/es unknown
  • 2010-05-14 AU AU2010247098A patent/AU2010247098A1/en not_active Abandoned
  • 2010-05-14 BR BRPI1007665A patent/BRPI1007665A2/pt not_active IP Right Cessation
  • 2010-05-14 EA EA201190250A patent/EA201190250A1/ru unknown
  • 2010-05-14 AP AP2011006030A patent/AP2011006030A0/xx unknown
  • 2010-08-22 US US12/860,896 patent/US20100316751A1/en not_active Abandoned
• 2011
  • 2011-11-11 CL CL2011002841A patent/CL2011002841A1/es unknown
  • 2011-12-14 CO CO11172221A patent/CO6470891A2/es not_active Application Discontinuation
• 2016
  • 2016-02-29 US US15/056,303 patent/US10470467B2/en active Active
• 2019
  • 2019-10-15 US US16/653,044 patent/US20200037615A1/en not_active Abandoned

Patent Citations (8)

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