[go: up one dir, main page]

US20100267698A1 - Benzimidazole and Pyridylimidazole Derivatives - Google Patents

Benzimidazole and Pyridylimidazole Derivatives Download PDF

Info

Publication number
US20100267698A1
US20100267698A1 US12/652,435 US65243510A US2010267698A1 US 20100267698 A1 US20100267698 A1 US 20100267698A1 US 65243510 A US65243510 A US 65243510A US 2010267698 A1 US2010267698 A1 US 2010267698A1
Authority
US
United States
Prior art keywords
alkyl
cycloalkyl
mono
amino
alkoxy
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US12/652,435
Inventor
Guiying Li
Pamela Albaugh
Kevin S. Currie
Guolin Cai
Linda M. Gustavson
Kyungae Lee
Alan Hutchison
George D. Maynard
Jun Yuan
Ling Hong Xie
Manuka Ghosh
Nian Liu
George P. Luke
Scott Mitchell
Martin Patrick Allen
Spiros Liras
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Neurogen Corp
Original Assignee
Neurogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Neurogen Corp filed Critical Neurogen Corp
Priority to US12/652,435 priority Critical patent/US20100267698A1/en
Publication of US20100267698A1 publication Critical patent/US20100267698A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/04—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/04—Centrally acting analgesics, e.g. opioids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/18—Antipsychotics, i.e. neuroleptics; Drugs for mania or schizophrenia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/20—Hypnotics; Sedatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/22—Anxiolytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/24—Antidepressants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/26—Psychostimulants, e.g. nicotine, cocaine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D233/00—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings
    • C07D233/04—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member
    • C07D233/20—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member with substituted hydrocarbon radicals, directly attached to ring carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/14—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
    • C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
    • C07D403/04—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
    • C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
    • C07D403/06—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
    • C07D403/14—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/14—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D409/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms
    • C07D409/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D413/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings
    • C07D413/06—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D413/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
    • C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
    • C07D417/04—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
    • C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
    • C07D417/06—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
    • C07D417/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
    • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
    • C07D471/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D473/00—Heterocyclic compounds containing purine ring systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
    • C07D491/02—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains two hetero rings
    • C07D491/08—Bridged systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
    • C07D491/02—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains two hetero rings
    • C07D491/10—Spiro-condensed systems

Definitions

  • This invention relates to benzimidazole and pyridylimidazole derivatives, and, more specifically, to such derivatives that bind with high selectively and/or high affinity to the benzodiazepine site of GABA A receptors.
  • This invention also relates to pharmaceutical compositions comprising such compounds and to the use of such compounds in the treatment of central nervous system (CNS) diseases.
  • CNS central nervous system
  • the GABA A receptor superfamily represents one of the classes of receptors through which the major inhibitory neurotransmitter, ⁇ -aminobutyric acid, or GABA, acts. Widely, although unequally, distributed throughout the mammalian brain, GABA mediates many of its actions through a complex of proteins called the GABA A receptor, which causes alteration in chloride conductance and membrane polarization. In addition to being the site of neurotransmitter action, a number of drugs including the anxiolytic and sedating benzodiazepines bind to this receptor.
  • the GABA A receptor comprises a chloride channel that generally, but not invariably, opens in response to GABA, allowing chloride to enter the cell. This, in turn, effects a slowing of neuronal activity through hyperpolarization of the cell membrane potential.
  • GABA A receptors are composed of five protein subunits. A number of cDNAs for these GABA A receptor subunits have been cloned and their primary structures determined. While these subunits share a basic motif of 4 membrane-spanning helices, there is sufficient sequence diversity to classify them into several groups. To date at least 6 ⁇ , 3 ⁇ , 3 ⁇ , 1 ⁇ , 1 ⁇ and 2 ⁇ subunits have been identified. Native GABA A receptors are typically composed of 2 ⁇ , 2 ⁇ , and 1 ⁇ .
  • the GABA A receptor binding sites for GABA (2 per receptor complex) are formed by amino acids from the ⁇ and ⁇ subunits. Amino acids from the ⁇ and ⁇ subunits together form one benzodiazepine site per receptor. Benzodiazepines exert their pharmacological actions by interacting with the benzodiazepine binding sites associated with the GABA A receptor. In addition to the benzodiazepine site (sometimes referred to as the benzodiazepine or BDZ receptor), the GABA A receptor contains sites of interaction for several other classes of drugs. These include a steroid binding site, a picrotoxin site, and a barbiturate site.
  • the benzodiazepine site of the GABA A receptor is a distinct site on the receptor complex that does not overlap with the site of interaction for other classes of drugs that bind to the receptor or for GABA (see, e.g., Cooper, et al., The Biochemical Basis of Neuropharmacology, 6 th ed., 1991, pp. 145-148, Oxford University Press, New York).
  • GABA A receptor antagonists In a classic allosteric mechanism, the binding of a drug to the benzodiazepine site increases the affinity of the GABA receptor for GABA.
  • Benzodiazepines and related drugs that enhance the ability of GABA to open GABA A receptor channels are known as agonists or partial agonists depending on the level of GABA enhancement.
  • Other classes of drugs, such as ⁇ -carboline derivatives, that occupy the same site and negatively modulate the action of GABA are called inverse agonists.
  • a third class of compounds exists which occupy the same site as both the agonists and inverse agonists and yet have little or no effect on GABA activity. These compounds will, however, block the action of agonists or inverse agonists and are thus referred to as GABA A receptor antagonists.
  • GABA A selective ligands may also act to potentiate the effects of certain other CNS active compounds.
  • selective serotonin reuptake inhibitors SSRIs
  • SSRIs selective serotonin reuptake inhibitors
  • This invention provides benzimidazole and pyridylimidazole derivatives that bind to the benzodiazepine site of GABA A receptors, including human GABA A receptors.
  • Preferred compounds of the invention bind with high selectivity and/or high affinity to GABA A receptors.
  • Preferred compounds act as agonists, antagonists or inverse agonists of such receptors. As such, they are useful in the treatment of various CNS disorders.
  • the invention provides compounds of Formula I (shown below), and pharmaceutical compositions comprising compounds of Formula I.
  • the invention provides methods for synthesizing compounds of Formula I.
  • the invention further provides methods of treating patients suffering from certain CNS disorders with an effective amount of a compound of the invention.
  • the patient may be a human or other mammal.
  • Treatment of humans, domesticated companion animals (pets) or livestock animals suffering from certain CNS disorders with an effective amount of a compound of the invention is encompassed by the invention.
  • the invention provides methods of potentiating the actions of other CNS active compounds. These methods comprise administering an effective amount of a compound of the invention in conjunction with the administration of another CNS active compound.
  • this invention relates to the use of compounds of Formula I as probes for the localization of GABA A receptors in tissue sections.
  • the invention provides compounds of Formula I
  • Z 1 is nitrogen or CR 1 ;
  • Z 2 is nitrogen or CR 2 ;
  • Z 3 is nitrogen or CR 3 ; and
  • Z 4 is nitrogen or CR 4 ; provided that no more than two of Z 1 , Z 2 , Z 3 , and Z 4 are nitrogen.
  • R 1 , R 2 , R 3 , and R 4 are independently selected from
  • G is a bond, alkyl, —O—, —C( ⁇ O)—, or —CH 2 C( ⁇ O)—
  • R A is a saturated, partially unsaturated, or aromatic carbocycle, consisting of 1 ring or 2 fused, pendant, or spiro rings, each ring containing 0, 1, or 2 heteroatoms independently chosen from N, S, and O, said saturated, partially unsaturated, or aromatic carbocycle is optionally substituted with 1, 2, 3, or 4 of R 20 ,
  • R B and R C are independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, alkoxy, cycloalkyl, (cycloalkyl)alkyl, heterocycloalkyl, aryl, arylalkyl, alkanoyl, heteroaryl, and mono and dialkylaminoalkyl, each of which is optionally substituted by 1 or 2 substituents independently chosen from halogen, hydroxy, cyano, amino, nitro, alkoxy, and alkyl, R B and R C and the atom to which they are attached form a 4- to 10-membered monocyclic or bicyclic ring, which may contain:
  • R E and R F are independently selected at each occurrence from alkyl, cycloalkyl, heterocycloalkyl, alkoxy, mono- or dialkylamino, aryl, or heteroaryl each of which is optionally substituted by 1, 2, or 3 of R 30 .
  • R 20 in this aspect of the invention, is independently selected at each occurrence from the group consisting of: halogen; hydroxy; nitro; cyano; amino; alkyl; alkoxy optionally substituted with amino or mono- or dialkylamino; cycloalkyl; cycloalkylalkyl; cycloalkylalkoxy; alkenyl; alkynyl; haloalkyl; oxo; haloalkoxy; mono- and dialkylamino; aminoalkyl; and mono- and dialkylaminoalkyl.
  • R 30 is independently selected at each occurrence from halogen, hydroxy, nitro, cyano, amino, alkyl, alkoxy optionally substituted with amino or mono- or dialkylamino, cycloalkyl, cycloalkylalkyl, cycloalkylalkoxy, heterocycloalkyl, alkenyl, alkynyl, haloalkyl, haloalkoxy, oxo, mono- and dialkylamino, aminoalkyl, and mono- and dialkylaminoalkyl.
  • R 5 represents hydrogen or haloalkyl
  • R 5 represents alkyl, cycloalkyl, or (cycloalkyl)alkyl, each of which may contain one or more double or triple bonds, and each of which is optionally substituted with 1, 2, or 3 of R 30 , or
  • R 5 represents aryl, arylalkyl, heteroaryl, or heteroarylalkyl each of which is optionally substituted with 1, 2, or 3 substituents selected from the group consisting of haloalkyl, amino, —NH(R 10 ), —N(R 10 )(R 11 ), carboxamido, (R 10 )NHcarbonyl, (R 10 )(R 11 )Ncarbonyl, halogen, hydroxy, nitro, cyano, amino, alkyl, alkoxy optionally substituted with amino or mono- or dialkylamino, cycloalkyl, cycloalkylalkyl, cycloalkylalkoxy, heterocycloalkyl, alkenyl, alkynyl, haloalkyl, haloalkoxy, aminoalkyl, and mono- and dialkylaminoalkyl.
  • substituents selected from the group consisting of haloalkyl, amino, —NH(R 10 ),
  • Q represents —C(R 6 )(R 7 ) or oxygen, with the proviso that Q is not oxygen when X 2 is nitrogen.
  • R 6 and R 7 independently represent hydrogen, fluorine, or alkyl.
  • R is independently chosen at each occurrence from hydrogen, halogen, amino, alkyl, alkenyl, alkynyl, alkoxy, cycloalkyl, (cycloalkyl)alkyl, haloalkyl, haloalkoxy, carboxamido, and 3- to 7-membered carbocyclic or heterocyclic groups which are saturated, unsaturated, or aromatic, which may be further substituted with one or more substituents independently selected from halogen, oxo, hydroxy, alkyl, and alkoxy.
  • R′ is independently chosen at each occurrence from alkyl, hydrogen, cycloalkyl, cycloalkyl(alkyl), and 3- to 7-membered carbocyclic or heterocyclic groups which are saturated, unsaturated, or aromatic, which 3- to 7-membered carbocyclic or heterocyclic groups are optionally substituted with one or more substituents independently selected from halogen, oxo, hydroxy, alkyl, and alkoxy.
  • X 1 and X 2 independently represent nitrogen, carbon or CH.
  • Y is nitrogen, oxygen, carbon, —CH—, —CH 2 —, or absent.
  • W represents aryl or heteroaryl, wherein the aryl or heteroaryl group is optionally substituted with up to 4 groups independently selected from R 30 , —CO 2 H, —C( ⁇ O)OR E , —C( ⁇ O)NHR E , —C( ⁇ O)NR E R F , —C(O)R E , and —S(O) m R E , —OR E , where R 30 and R E are as defined above and m is 0, 1, or 2.
  • R 1 , R 2 , R 3 , and R 4 are independently selected from
  • G is (C 1 -C 6 )alkyl, —O—, —C( ⁇ O)—, or —CH 2 C( ⁇ O)—, and
  • R A is a saturated, partially unsaturated, or aromatic carbocycle, consisting of 1 ring or 2 fused, pendant, or spiro rings, each ring consisting of from 3 to 8 ring atoms, and each ring containing 0, 1, or 2 heteroatoms independently chosen from N, S, and O; said saturated, partially unsaturated, or aromatic carbocycle is optionally substituted with 1, 2, 3, or 4 of R 20 ,
  • J is N, CH, or C—(C 1 -C 6 )alkyl and R B and R C are independently selected from the group consisting of hydrogen, (C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, (C 1 -C 6 )alkoxy, (C 3 -C 8 )cycloalkyl, (C 3 -C 8 cycloalkyl) (C 1 -C 4 )alkyl, heterocycloalkyl, aryl, aryl(C 1 -C 4 )alkyl, (C 1 -C 6 )alkanoyl, heteroaryl, and mono and di(C 1 -C 6 )alkylamino(C 1 -C 6 )alkyl, each of which is optionally substituted by 1 or 2 substituents independently chosen from halogen, hydroxy, cyano, amino, nitro, C 1 -C 6 alk
  • R 20 is independently selected at each occurrence from the group consisting of halogen; hydroxy; nitro; cyano; amino; (C 1 -C 6 )alkyl; (C 1 -C 6 )alkoxy optionally substituted with amino or mono- or di(C 1 -C 6 )alkylamino; (C 3 -C 8 )cycloalkyl; (C 3 -C 8 )cycloalkyl(C 1 -C 4 )alkyl; (C 3 -C 8 )cycloalkyl(C 1 -C 4 )alkoxy; (C 2 -C 6 )alkenyl; (C 2 -C 6 )alkynyl; halo(C 1 -C 6 )alkyl; halo(C 1 -C 6 )alkoxy; oxo; mono- and di(C 1 -C 6 )alkylamino; amino(C 1 -C 6 )alkyl; and mono- and
  • R 30 is independently selected at each occurrence from halogen, hydroxy, nitro, cyano, amino, (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy optionally substituted with amino or mono- or di(C 1 -C 6 )alkylamino, (C 3 -C 8 )cycloalkyl, (C 3 -C 8 )cycloalkyl(C 1 -C 4 )alkyl, (C 3 -C 8 )cycloalkyl(C 1 -C 4 )alkoxy, heterocycloalkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, halo(C 1 -C 6 )alkyl, halo(C 1 -C 10 )alkoxy, oxo, mono- and di(C 1 -C 6 )alkylamino, amino(C 1 -C 6 )alkyl, and mono
  • R 5 represents hydrogen or halo(C 1 -C 6 )alkyl
  • R 5 represents (C 1 -C 6 )alkyl, (C 3 -C 8 )cycloalkyl, or (C 3 -C 8 cycloalkyl) (C 1 -C 4 )alkyl, each of which may contain one or more double or triple bonds, and each of which is optionally substituted with 1, 2, or 3 of R 30 , or
  • R 5 represents aryl, aryl(C 1 -C 4 )alkyl, heteroaryl, or heteroaryl(C 1 -C 4 )alkyl each of which is optionally substituted with 1, 2, or 3 substituents selected from the group consisting of: halo(C 1 -C 6 )alkyl, amino, NH(R 10 ), N(R 10 )(R 11 ), carboxamido, NH(R 10 )carbonyl, N(R 10 )(R 11 )carbonyl, halogen, hydroxy, nitro, cyano, amino, (C 1 -C 6 )alkyl, (C 1 -C 8 )alkoxy optionally substituted with amino or mono- or di(C 1 -C 10 )alkylamino, (C 3 -C 10 )cycloalkyl, (C 3 -C 8 )cycloalkyl(C 1 -C 4 )alkyl, (C 3 -C 10 )cycl
  • Q represents —C(R 6 )(R 7 ) or oxygen, with the proviso that Q is not oxygen when X 2 is nitrogen;
  • R 6 and R 7 independently represent hydrogen, fluorine, or C 1 -C 6 alkyl
  • R is independently chosen at each occurrence from hydrogen, halogen, amino, C 1 -C 6 alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, C 1 -C 6 alkoxy, (C 3 -C 8 )cycloalkyl, (C 3 -C 8 cycloalkyl)(C 1 -C 4 )alkyl, halo(C 1 -C 6 )alkyl, haloalkoxy, carboxamido, and 3- to 7-membered carbocyclic or heterocyclic groups which are saturated, unsaturated, or aromatic, which may be further substituted with one or more substituents independently selected from halogen, oxo, hydroxy, C 1-4 alkyl, and —O(C 1-4 alkyl);
  • R′ is independently chosen at each occurrence from hydrogen, C 1 -C 6 alkyl, C 3 -C 8 cycloalkyl, C 3 -C 8 cycloalkyl(C 1 -C 4 alkyl), and 3- to 7-membered carbocyclic or heterocyclic groups which are saturated, unsaturated, or aromatic, which 3- to 7-membered carbocyclic or heterocyclic groups are optionally substituted with one or more substituents independently selected from halogen, oxo, hydroxy, C 1-4 alkyl, and —O(C 1-4 alkyl); and
  • X 1 , X 2 , W, and Y are as defined for Formula I, above.
  • Such compounds will be referred to as compounds of Formula IA.
  • a particular aspect of the invention is directed to compounds and pharmaceutically acceptable salts of Formula II
  • the variables Z 1 , Z 2 , Z 3 , Z 4 , R 5 , Q, X 1 , X 2 , and W carry the definition set forth for Formula I, or more preferably, for Formula IA;
  • X 3 and X 4 are independently selected from the group consisting of carbon, CR, N, O, S, NH, and N(C 1 -C 6 )alkyl; provided that at least one of X 1 , X 2 , X 3 , and X 4 is carbon or CR; and
  • R is independently chosen at each occurrence from hydrogen, halogen, amino, (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 3 -C 8 )cycloalkyl, (C 3 -C 8 )cycloalkyl(C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, halo(C 1 -C 6 )alkyl, halo(C 1 -C 6 )alkoxy, carboxamido, and 3- to 7-membered carbocyclic or heterocyclic groups which are saturated, unsaturated, or aromatic, which may be further substituted with one or more substituents independently selected from halogen, oxo, hydroxy, C 1-4 alkyl, and —O(C 1-4 alkyl).
  • the invention is particularly directed to compounds of Formula I, Formula IA, and Formula II, in which Z 1 is CR 1 , Z 2 is CR 2 , Z 3 is CR 3 , and Z 4 is CR 4 .
  • the invention is also directed to compounds of Formula I, Formula IA, and Formula II, in which one, and only one, of Z 1 , Z 2 , Z 3 , and Z 4 is nitrogen.
  • Another particular aspect of the invention provides compounds of Formula I, Formula IA, and Formula II, in which Z 1 is CR 1 , Z 4 is CR 4 , and only one, of Z 2 and Z 3 is nitrogen.
  • the invention is further directed to compounds of Formula I, Formula IA, and Formula II wherein:
  • X 2 is carbon; and Q is oxygen;
  • X 2 is carbon; and Q is C(R 6 )(R 7 );
  • X 1 is carbon; X 2 is N; and Q is C(R 6 )(R 7 )
  • X 1 is nitrogen; X 2 is carbon; and Q is C(R 5 ) (R 7 ); or
  • Q is C(R 6 )(R 7 )
  • preferred compounds are those where Z 1 is CR 1 , Z 2 is CR 2 , Z 3 is CR 3 , and Z 4 is CR 4 .
  • preferred compounds are those in which one, and only one, of Z 1 , Z 2 , Z 3 , and Z 4 is nitrogen.
  • compounds in which Z 1 is CR 1 , and only one, of Z 2 and Z 3 is nitrogen are particularly preferred.
  • the invention provides compounds of Formula III and Formula IV:
  • R, R 1 , R 2 , R 3 , R 4 , R 5 , Q, and W carry the definitions set forth for Formula I, or more preferably for Formula IA.
  • Particular compounds of Formula III included in the invention are those wherein Q is C(R 6 )(R 7 ).
  • Preferred compounds of Formula IV include those where R 6 and R 7 are hydrogen, methyl or fluoro and the other is ethyl, or where one of R 6 and R 7 is hydrogen, methyl or fluoro and the other is ethyl.
  • R 1 , R 2 , R 3 , and R 4 are independently selected from hydrogen, halogen, hydroxy, nitro, cyano, amino, (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 3 -C 8 )cycloalkyl, (C 3 -C 8 )cycloalkyl(C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, heterocycloalkyl, halo(C 1 -C 6 )alkyl, halo(C 1 -C 6 )alkoxy, mono or di(C 1 -C 6 )alkylamino, amino(C 1 -C 6 )alkyl, and mono- and di(C 1 -C 6 )alkylamino(C 1 -C 6 )alkyl;
  • R 5 represents hydrogen, (C 1 -C 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 3 -C 8 )cycloalkyl(C 1 -C 6 )alkyl, phenyl, benzyl, thiophenyl, thiazoyl, pyridyl, imidazolyl, pyrazolyl, or pyrimidinyl;
  • R 6 and R 7 independently represent hydrogen, fluorine, or C 1 -C 6 alkyl
  • W represents phenyl, thienyl, thiazoyl, pyridyl, imidazolyl, oxazolyl, triazolyl, tetrazolyl, pyrazolyl, isoxazolyl, or pyrimidinyl, each of which is optionally substituted with up to 4 R 30 groups, where R 30 carries the definition set forth for Formula I, or more preferably R 30 carries the definition set forth for Formula IA.
  • R, R 1 , R 2 , R 3 , R 4 , R 5 , Q, and W carry the definition set forth for Formula I, or more preferably for Formula IA, and
  • W represents a 6-membered aryl or heteroaryl groups, wherein the 6-membered aryl or heteroaryl group is optionally substituted with up to 4 groups independently selected from R 30 , —CO 2 H, —C( ⁇ O)OR E , —C( ⁇ O)NHR E , —C( ⁇ O)NR E R F , —C(O)R E , —S(O) m R E , and —OR E or wherein
  • W represents a 5-membered heteroaryl group, wherein the 5-membered heteroaryl group is optionally substituted with up to 4 groups independently selected from R 30 , —CO 2 H, —C( ⁇ O)OR E , —C( ⁇ O)NHR E , —C( ⁇ O)NR E R F , —C(O)R E , —S(O) n R E , and —OR E .
  • m is 0, 1, or 2
  • R E carries the definition set forth for Formula I, or more preferably R E carries the definition set forth for Formula IA and R 30 carries the definition set forth above with respect to Formula IA.
  • the compounds of Formula III or Formula IV are those in which one of R 2 or R 3 carries the definition set forth for Formula I, or more preferably for Formula IA.
  • R is independently selected at each occurrence from the group consisting of hydrogen, halogen, and (C 1 -C 2 )alkyl;
  • R 1 , R 4 , and the other of R 2 and R 3 are independently selected from hydrogen, halogen, hydroxy, nitro, cyano, amino, (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 3 -C 8 )cycloalkyl, (C 3 -C 8 )cycloalkyl(C 1 -C 6 )alkyl, halo(C 1 -C 6 )alkyl, halo(C 1 -C 6 )alkoxy, mono or di(C 1 -C 6 )alkylamino, amino(C 1 -C 6 )alkyl, and mono- and di(C 1 -C 6 )alkylamino(C 1 -C 6 )alkyl;
  • R 5 represents (C 1 -C 6 )alkyl
  • W represents phenyl, furanyl, thienyl, thiazoyl, pyridyl, imidazolyl, oxazolyl, triazolyl, tetrazolyl, pyrazolyl, isoxazolyl, pyrimidinyl, benzimidazolyl, quinolinyl, isoquinolinyl each of which is optionally substituted with up to 4 R 30 groups, where R 30 carries the definition set forth for Formula I, or more preferably R 30 carries the definition set forth for Formula IA.
  • Still other preferred W groups are 4-pyrimidinyl, 5-halo-2-pyrimidinyl, 3,6-dihalopyrimidin-2-yl, and 2,6-, 4,6-, and 5,6-dihalopyridin-2-yl.
  • Other preferred W groups are phenyl substituted with one or two independently selected C 1 -C 2 alkyl, C 1 -C 2 alkoxy, amino, halogen, trifluoromethyl, or cyano groups.
  • Still other preferred W groups are 2-thiazolyl groups carrying one or two independently selected C 1 -C 2 alkyl, amino, (C 1 -C 3 ) alkyl, hydroxy, (C 1 -C 3 )alkyl, or trifluoromethyl groups.
  • Another aspect of the invention includes compounds of Formula III or Formula IV wherein
  • R is independently selected at each occurrence from the group consisting of hydrogen, halogen, and (C 1 -C 2 )alkyl;
  • R 1 , R 4 , and one of R 2 and R 3 are independently selected from hydrogen, halogen, trifluoromethyl, C 1 -C 2 alkyl, and cyano;
  • R 2 and R 3 carries the definition set forth for Formula I, or more preferably for Formula IA;
  • R 5 represents (C 1 -C 6 )alkyl, and preferably C 2 -C 4 alkyl.
  • R 1 and R 4 are hydrogen;
  • Preferred R groups are independently selected from hydrogen and C 1 -C 3 alkyl, more preferably hydrogen and methyl, and most preferably are hydrogen.
  • R 5 groups are ethyl and n-propyl.
  • W is phenyl, pyridyl, or thiazolyl, each which is optionally substituted by one or more substituents independently chosen from halogen, cyano, hydroxy, oxo, C 1 -C 2 haloalkyl, C 1 -C 2 alkyl, and C 1 -C 2 alkoxy, or more preferably
  • W is 2-thiazolyl, 2-pyrimidinyl, 3-fluorophenyl, or 6-fluoro-2-pyridinyl. Such compounds will be referred to as compounds of Formula III-A and Formula IV-A.
  • a particular aspect of the invention provides compounds of Formula III-A and Formula IV-A, wherein:
  • R 2 and R 3 is chosen from hydrogen, halogen, hydroxy, nitro, cyano, amino, (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 3 -C 8 )cycloalkyl, (C 3 -C 8 )cycloalkyl(C 1 -C 6 )alkyl, halo(C 1 -C 6 )alkyl, halo(C 1 -C 6 )alkoxy, mono or di(C 1 -C 6 )alkylamino, amino(C 1 -C 6 )alkyl, and mono- and di(C 1 -C 6 )alkylamino(C 1 -C 6 )alkyl;
  • preferred compounds of Formula III-A and IV-A include those where one of R 2 and R 3 is hydrogen, halogen, hydroxy, nitro, cyano, amino, C 1 -C 3 alkyl, C 1 -C 2 alkoxy, cyclopropyl, cyclopropylmethyl, trifluoromethyl, or mono- or di(C 1 -C 2 )alkylamino, and the other is hydrogen, halogen, or C 1 -C 3 alkyl, preferably hydrogen or methyl.
  • More preferred compounds of Formula IV-A include those where R 2 is hydrogen, halogen, more preferably fluoro or chloro, cyano, amino, C 1 -C 2 alkyl or C 1 -C 2 alkoxy and R 3 is hydrogen or methyl.
  • R 2 is hydrogen, methyl, or ethyl
  • R 3 is hydrogen, halogen, preferably fluoro or chloro, cyano, amino, or C 1 -C 3 alkoxy.
  • Another aspect of the invention provides compounds of Formula III-A and Formula IV-A, wherein:
  • R 2 and R 3 is chosen from hydrogen, halogen, hydroxy, nitro, cyano, amino, (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 3 -C 8 )cycloalkyl, (C 3 -C 8 )cycloalkyl(C 1 -C 6 )alkyl, halo(C 1 -C 6 )alkyl, halo(C 1 -C 6 )alkoxy, mono or di(C 1 -C 6 )alkylamino, amino(C 1 -C 6 )alkyl, and mono- and di(C 1 -C 6 )alkylamino(C 1 -C 6 )alkyl;
  • J is N, CH, or C— (C 1 -C 6 )alkyl
  • R 20 carries the definition set forth with respect to Formula I, or more preferably R 20 carries the definition set forth for R 20 Formula IA.
  • the invention also provides compounds of Formula III-A and Formula IV-A, wherein:
  • R 2 and R 3 is chosen from hydrogen, halogen, hydroxy, nitro, cyano, amino, (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 3 -C 8 )cycloalkyl, (C 3 -C 8 )cycloalkyl(C 1 -C 6 )alkyl, halo(C 1 -C 6 )alkyl, halo(C 1 -C 6 )alkoxy, mono or di(C 1 -C 6 )alkylamino, amino(C 1 -C 6 )alkyl, and mono- and di(C 1 -C 6 )alkylamino(C 1 -C 6 )alkyl;
  • R 2 and R 3 is a group of the formula:
  • G is a bond or C 1 -C 2 alkyl
  • R A is a saturated, partially unsaturated, or aromatic carbocycle, consisting of 1 ring or 2 fused, pendant, or spiro rings, each ring containing 0, 1, or 2 heteroatoms independently chosen from N, S, and O, said saturated, partially unsaturated, or aromatic carbocycle is optionally substituted with 1, 2, 3, or 4 of R 20 ;
  • R 20 carries the definition set forth for R a in Formula I, or more preferably R 20 carries the definition set forth for R 20 in Formula IA R 20 .
  • R A is chosen from phenyl, pyrrolyl, pyrazolyl, thiazolyl, isoxazolyl, triazolyl, tetrazolyl, oxadiazolyl, and oxazolyl each of which is optionally substituted with 1, 2, 3, or 4 of R 20 .
  • R 2 and R 3 is chosen from hydrogen, halogen, hydroxy, nitro, cyano, amino, (C 1 -C 6 )alkyl, (C 1 -C 5 )alkoxy, (C 3 -C 8 )cycloalkyl, (C 3 -C 8 )cycloalkyl(C 1 -C 6 )alkyl, halo(C 1 -C 6 )alkyl, halo(C 1 -C 6 )alkoxy, mono or di(C 1 -C 6 )alkylamino, amino(C 1 -C 6 )alkyl, and mono- and di(C 1 -C 6 )alkylamino(C 1 -C 6 )alkyl; and
  • R 2 and R 3 is —HC ⁇ N—OH or —HC ⁇ N(C 1 -C 6 alkoxy).
  • Another aspect of the invention is directed to compounds of Formula I, Formula IA and Formula II wherein
  • Z 1 is CR 1
  • Z 4 is CR 4
  • either Z 2 or Z 3 is nitrogen
  • W represents a 5-membered heteroaryl group, and the 5-membered heteroaryl group is optionally substituted with up to 4 groups independently selected from R 30 , —CO 2 H, —C( ⁇ O)OR E , —C( ⁇ O)NHR E , —C( ⁇ O)NR E R F , —C(O)R E , and —S(O) n R E , —OR E ; or
  • W represents a 6-membered aryl or heteroaryl group, wherein the 6-membered aryl or heteroaryl group is optionally substituted with up to 4 groups independently selected from R 30 , —CO 2 H, —C( ⁇ O)OR E , —C( ⁇ O)NHR E , —C( ⁇ O)NR E R F , —C(O)R E , and —S(O) n R E , —OR E ;
  • R 30 and R E carry the definitions set forth for those groups in Formula I, or preferably for Formula IA, and m is 0, 1, or 2.
  • the invention includes compounds represented by Formula V and Formula VI
  • R, R 1 , R 2 , R 3 , R 4 , R 5 , and Q carry the definitions set forth for Formula I, or more preferably for Formula IA, and W is a 5-membered heteroaryl group as described above.
  • the invention further includes compounds of Formula V and Formula VI wherein
  • R is independently selected at each occurrence from the group consisting of
  • R 1 , R 2 , R 3 , and R 4 are independently selected from hydrogen, halogen, hydroxy, nitro, cyano, amino, (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 3 -C 8 )cycloalkyl, (C 3 -C 8 )cycloalkyl(C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, heterocycloalkyl, halo(C 1 -C 6 )alkyl, halo(C 1 -C 6 )alkoxy, mono or di(C 1 -C 6 )alkylamino, amino(C 1 -C 6 )alkyl, and mono- and di(C 1 -C 6 )alkylamino(C 1 -C 6 )alkyl;
  • R 5 represents hydrogen, (C 1 -C 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 3 -C 8 )cycloalkyl(C 1 -C 6 )alkyl, phenyl, benzyl, thiophenyl, thiazoyl, pyridyl, imidazolyl, pyrazolyl, or pyrimidinyl;
  • R 6 and R 7 independently represent hydrogen, fluorine, or C 1 -C 6 alkyl
  • W represents either a 5-membered heteroaryl group chosen from thienyl, thiazolyl, imidazolyl, oxazolyl, triazolyl, tetrazolyl, pyrazolyl, or isoxazolyl each of which is optionally substituted with up to 4 R 30 groups; or
  • W represents a 6-membered aryl or heteroaryl group chosen from phenyl, pyrimidinyl, pyridyl, pyridizinyl, or pyrazinyl, each of which is optionally substituted with up to 4 R 30 groups;
  • R 30 is as defined for Formula I, or preferably as defined for Formula IA.
  • Preferred compounds of Formula V an VI include those where R 2 and R 3 independently represent hydrogen, halogen, preferably fluoro or chloro, C 1 -C 3 alkyl, cyclopropyl, cyclopropylmethyl, C 1 -C 3 alkoxy, trifluoromethyl, nitro, cyano, amino, or mono- or di(C 1 -C 3 )alkylamino.
  • R 2 and R 3 groups are mono- or di(C 1 -C 3 )alkylamino(C 2 -C 3 )alkoxy, morpholinyl(C 2 -C 3 )alkoxy, piperidin-1-yl(C 2 -C 3 )alkoxy, and piperazin-1-yl(C 2 -C 3 )alkoxy.
  • the invention is directed to compounds of Formula V and Formula VI wherein:
  • R is independently selected at each occurrence from the group consisting of hydrogen, halogen, and (C 1 -C 2 )alkyl;
  • R 1 and R 4 are independently selected from hydrogen, halogen, hydroxy, nitro, cyano, amino, (C 1 -C 8 )alkyl, (C 1 -C 6 )alkoxy, (C 3 -C 8 )cycloalkyl, (C 3 -C 8 )cycloalkyl(C 1 -C 6 )alkyl, halo(C 1 -C 6 )alkyl, halo(C 1 -C 6 )alkoxy, mono or di(C 1 -C 6 )alkylamino, amino(C 1 -C 6 )alkyl, and mono- and di(C 1 -C 6 )alkylamino(C 1 -C 6 )alkyl.
  • R 2 (Formula V) and R 3 (Formula VI) carry the definitions set forth with respect to Formula I, or more preferably for Formula IA;
  • R 5 represents (C 1 -C 10 )alkyl, preferably ethyl or n-propyl;
  • R 6 and R 7 are hydrogen
  • W represents a 5-membered heteroaryl group chosen from furanyl, thienyl, thiazoyl, imidazolyl, oxazolyl, triazolyl, tetrazolyl, pyrazolyl, or isoxazolyl, each of which is optionally substituted with up to 4 R 30 groups, or
  • W represents a 6-membered aryl or heteroaryl group chosen from phenyl, pyrimidinyl, pyridyl, pyridizinyl, or pyrazinyl each of which is optionally substituted with up to 4 R 30 groups;
  • R 30 is as defined for Formula I, or more preferably for Formula IA.
  • Preferred R groups are hydrogen and C 1 -C 3 alkyl, more preferably hydrogen and methyl, and most preferably hydrogen.
  • R 1 and R 4 groups for this aspect of the invention include hydrogen, halogen, trifluoromethyl, C 1 -C 2 alkyl, and cyano.
  • R 1 and R 4 are hydrogen. More preferably R, R 1 , and R 4 are all hydrogen.
  • Preferred compounds of this aspect of the invention in which W is a 5-membered heteroaryl group include those wherein W is thiazolyl which is optionally substituted by one or more substituents independently chosen from halogen, cyano, hydroxy, oxo, C 1 -C 2 haloalkyl, C 1 -C 2 alkyl, and C 1 -C 2 alkoxy.
  • Other preferred compounds are those wherein W is 2-thiazolyl.
  • W is a 6-membered heteroaryl group
  • W is phenyl or pyridyl, each of which is optionally substituted by one or more substituents independently chosen from halogen, cyano, hydroxy, oxo, C 1 -C 2 haloalkyl, C 1 -C 2 alkyl, and C 1 -C 2 alkoxy.
  • W is 2-pyrimidinyl, 3-fluorophenyl, or 6-fluoro-2-pyridinyl.
  • Still other preferred W groups are 4-pyrimidinyl, 5-halo-2-pyrimidinyl, 3,6-dihalopyrimidin-2-yl, and 2,6-, 4,6-, and 5,6-dihalopyridin-2-yl.
  • Other preferred W groups are phenyl substituted with one or two independently selected C 1 -C 2 alkyl, C 1 -C 2 alkoxy, amino, halogen, trifluoromethyl, or cyano groups.
  • Still other preferred W groups are 2-thiazolyl groups carrying one or two independently selected C 1 -C 2 alkyl, amino, (C 1 -C 3 ) alkyl, hydroxy, (C 1 -C 3 )alkyl, or trifluoromethyl groups.
  • R 2 (for Formula V-A) or R 3 (for Formula VI-A) is chosen from
  • R 2 for Formula V-A
  • R 3 for Formula VI-A
  • R 2 is chosen from hydrogen, halogen, hydroxy, nitro, cyano, amino, halo(C 1 -C 6 )alkyl, and halo(C 1 -C 6 )alkoxy.
  • J is N, CH, or C—(C 1 -C 10 )alkyl
  • R B and R C are independently selected from the group consisting of hydrogen, (C 1 -C 6 )alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, C 3 -C 8 )cycloalkyl, and (C 3 -C 8 cycloalkyl) (C 1 -C 4 )alkyl; or
  • R B and R C and the atom to which they are attached form a 4- to 10-membered monocyclic or bicyclic ring, which may contain a) one or more double bonds, b) one or more of oxo, O, S, SO, SO 2 , and N—R D wherein R D is hydrogen or (C 1 -C 6 )alkyl; and/or c) one or more substituents R 20 , where R 20 carries the definition set forth for compounds of Formula I, or more preferably that set forth for compounds of Formula IA.
  • R 2 for Formula V-A
  • R 3 for Formula VI-A
  • G is a bond or C 1 -C 2 alkyl
  • R A is a saturated, partially unsaturated, or aromatic carbocycle consisting of 1 ring or 2 fused, pendant, or spiro rings, each ring containing 0, 1, or 2 heteroatoms independently selected from N, S, and O, where the saturated, partially unsaturated, or aromatic carbocycle is optionally substituted with 1, 2, 3, or 4 of R 20 .
  • Preferred compounds of this class are those where R A is chosen from phenyl, pyrrolyl, pyrazolyl, thiazolyl, isoxazolyl, triazolyl, tetrazolyl, oxadiazolyl, and oxazolyl each of which is optionally substituted with 1, 2, 3, or 4 of R 20 .
  • R 20 carries the definition set forth for compounds of Formula I, or more preferably that set forth for compounds of Formula IA.
  • the invention also includes compounds of Formula V-A and Formula VI-A wherein R 2 (for Formula V-A) or R 3 (for Formula VI-A) is —HC ⁇ N—OH or —HC ⁇ N(C 1 -C 6 alkoxy).
  • Particularly embodied in the invention are compounds of Formulae X-XVIII wherein Q (when present) is C(R 6 )(R 7 ).
  • Q when present is C(R 6 )(R 7 ).
  • R 6 and R 7 are hydrogen.
  • w represents a 5-membered heteroaryl group
  • the 5-membered heteroaryl group is optionally substituted with up to 4 groups independently selected from R 30 , —CO 2 H, —C( ⁇ O)OR E , —C( ⁇ O)NHR E , —C( ⁇ O)NR E R F , —C(O)R E , and —S(O) n R E , —OR E , where R 30 and R E are as defined above and m is 0, 1, or 2.
  • Preferred compounds of this class are compounds wherein wherein Z 1 is CR 1 , Z 2 is CR 2 , Z 3 is CR 3 , and Z 4 is CR 4 or wherein one and only one Z 1 , Z 2 , Z 3 , Z 4 is nitrogen are also preferred; compounds of this class wherein one and only one Z 1 , Z 2 , Z 3 , Z 4 is nitrogen, and either Z 2 or Z 3 is nitrogen are particularly preferred.
  • This invention provides benzimidazole and pyridylimidazole derivatives, preferred examples of which bind with high affinity to the benzodiazepine site of GABA A receptors, including human GABA A receptors.
  • the affinity of compounds of Formula I for the benzodiazepine site may be determined using a GABA A receptor binding assay, such as the assay presented in Example 53.
  • Preferred compounds of Formula I that bind with high affinity to the benzodiazepine site of the GABA A receptor exhibit K i values of less than 1 ⁇ M in that assay
  • Very high affinity compounds of the invention exhibit K i values of less than 100 nM or more preferably less than 10 nM in the assay presented in Example 53.
  • Benzimidazole and pyridylimidazole derivatives that bind with high selectivity to the benzodiazepine site of GABA A receptors, including human GABA A receptors are also included in this invention.
  • Preferred compounds of Formula I which exhibit high selectivity (or high specificity) exhibit affinity for the benzodiazepine site of the GABA receptor that is at least 10-fold greater, and preferably 100-fold greater, than the affinity exhibited at any other membrane-bound receptor which is a known drug target. More preferred compounds of Formula I do not exhibit a binding affinity at any other membrane-bound receptor which is a known drug target that is less than 1 micromolar.
  • Membrane-bound receptors that are known drug targets include, but are not limited to dopamine receptors, CRF receptors, bradykinin receptors, NPY receptors, beta-adrenergic receptors, capsaicin receptors, galanin receptors, MCH receptors, melanocortin receptors, and neurokinin receptors. Binding affinities for membrane-bound receptors which are known drug targets may be determined via radioligand binding assays which are generally well known in the art.
  • the invention further comprises methods of treating patients in need of such treatment with an amount of a compound of the invention sufficient to alter the symptoms of a CNS disorder.
  • Compounds of the inventions that act as agonists at ⁇ 2 ⁇ 3 ⁇ 2 and ⁇ 3 ⁇ 3 ⁇ 2 receptor subtypes are useful in treating anxiety disorders such as panic disorder, obsessive compulsive disorder and generalized anxiety disorder; stress disorders including post-traumatic stress, and acute stress disorders.
  • Compounds of the inventions that act as agonists at ⁇ 2 ⁇ 3 ⁇ 2 and ⁇ 3 ⁇ 3 ⁇ 2 receptor subtypes are also useful in treating depressive or bipolar disorders and in treating sleep disorders.
  • Compounds of the invention that act as inverse agonists at the ⁇ 5 ⁇ 3 ⁇ 2 receptor subtype or ⁇ 1 ⁇ 2 ⁇ 2 and ⁇ 5 ⁇ 3 ⁇ 2 receptor subtypes are useful in treating cognitive disorders including those resulting from Down Syndrome, neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease, and stroke related dementia.
  • Compounds of the invention that act as inverse agonists at the ⁇ 5 ⁇ 3 ⁇ 2 are particularly useful in treating cognitive disorders through the enhancement of memory, and particularly short-term memory, in memory-impaired patients.
  • Compounds of the invention that act as agonists at the ⁇ 1 ⁇ 2 ⁇ 2 receptor subtype are useful in treating convulsive disorders such as epilepsy.
  • Compounds that act as antagonists at the benzodiazepine site are useful in reversing the effect of benzodiazepine overdose and in treating drug and alcohol addiction.
  • the diseases and/or disorders that can also be treated using compounds and compositions according to the invention include:
  • Depression e.g. depression, atypical depression, bipolar disorder, depressed phase of bipolar disorder.
  • Anxiety e.g. general anxiety disorder (GAD), agoraphobia, panic disorder +/ ⁇ agoraphobia, social phobia, specific phobia, Post traumatic stress disorder, obsessive compulsive disorder (OCD), dysthymia, adjustment disorders with disturbance of mood and anxiety, separation anxiety disorder, anticipatory anxiety acute stress disorder, adjustment disorders, cyclothymia.
  • Sleep disorders e.g. sleep disorders including primary insomnia, circadian rhythm sleep disorder, dyssomnia NOS, parasomnias, including nightmare disorder, sleep terror disorder, sleep disorders secondary to depression and/or anxiety or other mental disorders, substance induced sleep disorder.
  • Cognition Impairment e.g. cognition impairment, memory impairment, short-term memory impairment, Alzheimer's disease, Parkinson's disease, mild cognitive impairment (MC1), age-related cognitive decline (ARCD), stroke, traumatic brain injury, AIDS associated dementia, and dementia associated with depression, anxiety or psychosis.
  • Attention Deficit Disorder e.g. attention deficit disorder (ADD), and attention deficit and hyperactivity disorder (ADHD).
  • Speech disorders e.g. stuttering, including motor tic, clonic stuttering, dysfluency, speech blockage, dysarthria, Tourete syndrome or logospasm.
  • Psychosis e.g. schizophrenia, hallucinatory disorders
  • the invention also provides pharmaceutical compositions comprising one or more compounds of the invention together with a pharmaceutically acceptable carrier or excipient, for treating disorders responsive to GABA A receptor modulation, e.g., treatment of anxiety, depression, sleep disorders or cognitive impairment by GABA A receptor modulation.
  • Pharmaceutical compositions include packaged pharmaceutical compositions comprising a container holding a therapeutically effective amount of at least one GABA A receptor modulator as described supra and instructions (e.g., labeling) indicating the contained GABA A receptor ligand is to be used for treating a disorder responsive to GABA A receptor modulation in the patient.
  • the invention provides a method of potentiating the actions of other CNS active compounds, which comprises administering an effective amount of a compound of the invention in combination with another CNS active compound.
  • CNS active compounds include, but are not limited to the following: for anxiety, serotonin receptor (e.g. 5-HT 1A ) agonists and antagonists; for anxiety and depression, neurokinin receptor antagonists or corticotropin releasing factor receptor (CRF 1 ) antagonists; for sleep disorders, melatonin receptor agonists; and for neurodegenerative disorders, such as Alzheimer's dementia, nicotinic agonists, muscarinic agents, acetylcholinesterase inhibitors and dopamine receptor agonists.
  • SSRIs selective serotonin reuptake inhibitors
  • Combination administration can be carried out in a fashion analogous to that disclosed in Da-Rocha, et al., J. Psychopharmacology (1997) 11(3) 211-218; Smith, et al., Am. J. Psychiatry (1998) 155(10) 1339-45; or Le, et al., Alcohol and Alcoholism (1996) 31 Suppl. 127-132.
  • the present invention also pertains to methods of inhibiting the binding of benzodiazepine compounds, such as Ro15-1788, or GABA to the GABA A receptors which methods involve contacting a solution containing compound of the invention with cells expressing GABA A receptors, wherein the compound is present at a concentration sufficient to inhibit benzodiazepine binding or GABA binding to GABA A receptors in vitro.
  • This method includes inhibiting the binding of benzodiazepine compounds to GABA A receptors in vivo, e.g., in a patient given an amount of a compound of Formula I that would be sufficient to inhibit the binding of benzodiazepine compounds or GABA to GABA A receptors in vitro.
  • such methods are useful in treating benzodiazepine drug overdose.
  • the amount of a compound that would be sufficient to inhibit the binding of a benzodiazepine compound to the GABA A receptor may be readily determined via a GABA A receptor binding assay, such as the assay described in Example 53.
  • the GABA A receptors used to determine in vitro binding may be obtained from a variety of sources, for example from preparations of rat cortex or from cells expressing cloned human GABA A receptors.
  • the invention also provides methods for altering the signal-transducing activity, particularly the chloride ion conductance of GABA A receptors, said method comprising exposing cells expressing such receptors to an effective amount of a compound of the invention.
  • This method includes altering the signal-transducing activity of GABA A receptors in vivo, e.g., in a patient given an amount of a compound of Formula I that would be sufficient to alter the signal-transducing activity of GABA A receptors in vitro.
  • the amount of a compound that would be sufficient to alter the signal-transducing activity of GABA A receptors may be determined via a GABA A receptor signal transduction assay, such as the assay described in Example 54.
  • the cells expressing the GABA receptors in vivo may be, but are not limited to, neuronal cells or brain cells. Such cells may be contacted with compounds of the invention through contact with a body fluid containing the compound, for example through contact with cerebrospinal fluid. Alteration of the signal-transducing activity of GABA A receptors in vitro may be determined from a detectable change in the electrophysiology of cells expressing GABA A receptors, when such cells are contacted with an compound of the invention in the presence of GABA. For example, a change in the electrophysiology of cells expressing GABA A receptors may be detected using a voltage-clamp assay performed on oocytes injected with GABA A receptor mRNA. Such an assay is shown in Example 54.
  • Intracellular recording or patch-clamp recording may be used to quantitate changes in electrophysiology of cells.
  • a reproducible change in behavior of an animal given a compound of the invention may also be used to indicate that changes in the electrophysiology of the animal's cells expressing GABA A receptors has occurred.
  • the GABA A receptor ligands provided by this invention and labeled derivatives thereof are also useful as standards and reagents in determining the ability of a potential pharmaceutical to bind to the GABA A receptor.
  • Radiolabeled derivatives the GABA A receptor ligands provided by this invention are also useful as radiotracers for positron emission tomography (PET) imaging or for single photon emission computerized tomography (SPECT).
  • PET positron emission tomography
  • SPECT single photon emission computerized tomography
  • More particularly compounds of the invention may be used for demonstrating the presence of GABA A receptors in cell or tissue samples. This may be done by preparing a plurality of matched cell or tissue samples, at least one of which is prepared as an experimental sample and at least one of which is prepared as a control sample.
  • the experimental sample is prepared by contacting (under conditions that permit binding of RO15-1788 to GABA A receptors within cell and tissue samples) at least one of the matched cell or tissue samples that has not previously been contacted with any compound or salt of the invention with an experimental solution comprising the detectably-labeled preparation of the selected compound or salt at the first measured molar concentration.
  • the control sample is prepared in the same manner as the experimental sample and also contains an unlabelled preparation of the same compound or salt of the invention at a greater molar concentration.
  • the experimental and control samples are then washed to remove unbound detectably-labeled compound.
  • the amount of remaining bound detectably-labeled compound is then measured and the amount of detectably-labeled compound in the experimental and control samples is compared.
  • a comparison that indicates the detection of a greater amount of detectable label in the at least one washed experimental sample than is detected in any of control samples demonstrates the presence of GABA A receptors in that experimental sample.
  • the detectably-labeled compound used in this procedure may be labeled with a radioactive label or a directly or indirectly luminescent label.
  • tissue sections are used in this procedure and the detectably-labeled compound is radiolabeled, the bound, labeled compound may be detected autoradiographically to generate an autoradiogram.
  • the amount of detectable label in an experimental or control sample may be measured by viewing the autoradiograms and comparing the exposure density of the autoradiograms.
  • the invention provides a method for preparing a compound of Formula A
  • Z 1 , Z 2 , Z 3 , Z 4 , and R 5 carry the definitions forth for Formula I, or more preferably Z 1 , Z 2 , Z 3 , Z 4 , and R 5 carry the definitions forth for Formula Ia.
  • R 6 and R 7 independently represent hydrogen, fluorine, or alkyl.
  • R in Formula B is independently chosen at each occurrence from hydrogen, halogen, amino, C 1 -C 6 alkyl, (C 2 -C 6 )alkenyl, (C 2 -C 6 )alkynyl, C 1 -C 6 alkoxy, (C 3 -C 8 )cycloalkyl, (C 3 -C 8 cycloalkyl) (C 1 -C 4 )alkyl, halo(C 1 -C 6 )alkyl, haloalkoxy, carboxamido, and 3- to 7-membered carbocyclic or heterocyclic groups which are saturated, unsaturated, or aromatic, which may be further substituted with one or more substituents independently selected from halogen, oxo, hydroxy, C 1-4 alkyl, and —O(C 1-4 alkyl).
  • W in Formula B represents aryl or heteroaryl, wherein the aryl or heteroaryl group is optionally substituted with up to 4 groups independently selected from R 30 , —CO 2 H, C( ⁇ O)OR E , —C( ⁇ O)NHR E , —C( ⁇ O)NR E R F , —C(O)R E , and —S(O) m R E , —OR E , where R 30 and R E are as for Formula I or preferably as defined for Formula Ia and m is 0, 1, or 2. This process will be referred to as Process 1.
  • the invention includes a process of preparing a compound of Formula A as described above wherein: Z 1 is CR 1 , Z 2 is CR 2 , Z 3 is CR 3 , and Z 4 is CR 4 .
  • R is independently selected at each occurrence from the group consisting of hydrogen, halogen, and (C 1 -C 2 )alkyl;
  • R 1 , R 4 , and one of R 2 and R 3 are independently selected from hydrogen, halogen, hydroxy, nitro, cyano, amino, (C 1 -C 5 )alkyl, (C 1 -C 6 )alkoxy, (C 3 -C 8 )cycloalkyl, (C 3 -C 8 )cycloalkyl(C 1 -C 6 )alkyl, halo(C 1 -C 6 )alkyl, halo(C 1 -C 6 )alkoxy, mono or di(C 1 -C 6 )alkylamino, amino(C 1 -C 6 )alkyl, and mono- and di(C 3 -C 6 )alkylamino(C 1 -C 6 )alkyl.
  • R 2 and R 3 carries the definition set forth for Formula I, or preferably that set forth for Formula Ia, or in certain preferred embodiments this group is chosen from
  • R, R 1 , and R 4 are all hydrogen.
  • R 5 represents (C 1 -C 6 )alkyl. Preferred definitions of R 5 include ethyl and n-propyl.
  • R 6 and R 7 are hydrogen.
  • W represents phenyl, furanyl, thienyl, thiazolyl, imidazolyl, oxazolyl, triazolyl, tetrazolyl, pyrazolyl, isoxazolyl, pyrimidinyl, benzimidazolyl, quinolinyl, isoquinolinyl each of which is optionally substituted with up to 4 R 30 groups, where R 30 is as defined in the above process.
  • Preferred W groups include, 2-thiazolyl, 2-pyrimidinyl, 3-fluorophenyl, or 6-fluoro-2-pyridinyl.
  • the invention is directed to a process, as described as for Process 1, wherein Z 1 is CR 1 ; one and only one of Z 2 or Z 2 is nitrogen; Z 4 is CR 4 .
  • R 1 and R 4 may carry the definition set forth in Process 1.
  • Preferred definitions of R 1 and R 4 include hydrogen, halogen, trifluoromethyl, C 1 -C 2 alkyl, and cyano.
  • R, R 1 , and R 4 are all hydrogen.
  • R 2 or R 3 (for whichever one of Z 2 or Z 3 is CR 2 or CR 3 ) is chosen from
  • R 5 represents (C 1 -C 6 )alkyl. Preferred definitions of R 5 include ethyl and n-propyl.
  • R 6 and R 7 are hydrogen.
  • W represents a 5-membered heteroaryl group, the 5-membered heteroaryl group is optionally substituted with up to 4 groups independently selected from R 30 , —CO 2 H, —C( ⁇ O)OR E , —C( ⁇ O)NHR E , —C( ⁇ O)NR E R F , —C(O)R E , and —S(O) m R E , —OR E , where R 30 and R E are as defined above and m is 0, 1, or 2; or
  • W represents a 6-membered aryl or heteroaryl group, wherein the 6-membered aryl or heteroaryl group is optionally substituted with up to 4 groups independently selected from R 30 , —CO 2 H, —C( ⁇ O)OR E , —C( ⁇ O)NHR E , —C( ⁇ O)NR E R F , —C(O)R E , and —S(O) m R E , —OR E , where R 30 and R E are as defined above and m is 0, 1, or 2.
  • W represents a 5-membered heteroaryl group
  • W is preferably thiazolyl, thienyl, imidazolyl, oxazolyl, triazolyl, tetrazolyl, pyrazolyl, or isoxazolyl, each of which is optionally substituted by one or more substituents independently chosen from halogen, cyano, hydroxy, oxo, C 1 -C 2 haloalkyl, C 1 -C 2 alkyl, and C 1 -C 2 alkoxy.
  • Unsubstituted 2-thiazolyl is a particularly preferred W group.
  • W represents a 6-membered aryl or heteroaryl group
  • W is preferably phenyl, pyrimidinyl, pyridyl, pyrazinyl, or pyridizinyl, each of which is optionally substituted by one or more substituents independently chosen from halogen, cyano, hydroxy, oxo, C 1 -C 2 haloalkyl, C 1 -C 2 alkyl, and C 1 -C 2 alkoxy.
  • Particularly preferred W groups include 2-pyrimidinyl, 3-fluorophenyl, or 6-fluoro-2-pyridinyl.
  • the reactants B and C are generally combined in a polar aprotic solvent, such as THF, DMF, or 1,4-dioxane, at temperatures ranging from 0-100 degrees C.
  • a reducing agent such as NaH or other base, for example sodium hydroxide, potassium butoxide, potassium carbonate, or cesium carbonate, is then added, and the reaction is allowed to proceed.
  • Choice of solvent, reaction temperature, and reducing agent will depend on the identity of the reactants B and C, but will be readily determined by a worker of ordinary skill in the art of chemical synthesis.
  • Scheme I, step 4 provides further illustration of this process.
  • Formula I includes, but is not limited to the subformulae exemplified as Formula Ia, Formulae II-VI and Formulae X-XVIII and their pharmaceutically acceptable acid and base addition salts.
  • phrases “pharmaceutically acceptable” is employed herein to refer to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings or animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio.
  • pharmaceutically acceptable salts refer to derivatives of the disclosed compounds wherein the parent compound is modified by making acid or base salts thereof. Those skilled in the art will recognize a wide variety of non-toxic pharmaceutically acceptable addition salts.
  • Examples of pharmaceutically acceptable salts include, but are not limited to, mineral or organic acid salts of basic residues such as amines; alkali or organic salts of acidic residues such as carboxylic acids; and the like.
  • the pharmaceutically acceptable salts include the conventional non-toxic salts or the quaternary ammonium salts of the parent compound formed, for example, from non-toxic inorganic or organic acids.
  • such conventional non-toxic salts include those derived from inorganic acids such as hydrochloric, hydrobromic, hydroiodic, sulfuric, sulfamic, sulfinic, phosphoric, nitric and the like; and the salts prepared from organic acids such as alkanoic such as acetic, HOOC—(CH 2 ) n -ACOOH where n is 0-4, and the like, tartaric, maleic, propionic, succinic, glycolic, stearic, lactic, malic, tartaric, citric, ascorbic, pamoic, malefic, hydroxymaleic, phenylacetic, glutamic, benzoic, salicylic, sulfanilic, 2-acetoxybenzoic, fumaric, toluenesulfonic, methanesulfonic, ethane disulfonic, oxalic, isethionic, HOOC—(CH 2 ) n —COOH where n
  • the pharmaceutically acceptable salts of the present invention can be synthesized from the parent compound which contains a basic or acidic moiety by conventional chemical methods.
  • such salts can be prepared by reacting the free acid or base forms of these compounds with a stoichiometric amount of the appropriate base or acid in water or in an organic solvent, or in a mixture of the two; generally, nonaqueous media like ether, ethyl acetate, ethanol, isopropanol, or acetonitrile are preferred. Lists of suitable salts are found in Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, Pa., p. 1418 (1985).
  • the invention includes hydrates of compounds of Formula I.
  • the invention includes all crystalline forms of the compounds of Formula I. Certain crystalline forms may be preferred.
  • the present invention also encompasses the acylated prodrugs of the compounds of Formula I.
  • acylated prodrugs of the compounds of Formula I Those skilled in the art will recognize various synthetic methodologies that may be employed to prepare non-toxic pharmaceutically acceptable addition salts and acylated prodrugs of the compounds encompassed by Formula I.
  • the invention further encompasses all enantiomers and diastereomers of the disclosed compounds. Those of ordinary skill in the art will readily recognize methods by which mixtures of enantiomers and diasteromers may be resolved.
  • the definition of Formula I as used in herein include possible isomers, such as tautomers and rotamers.
  • the compounds herein described may have one or more asymmetric centers or planes.
  • Compounds of the present invention containing an asymmetrically substituted atom may be isolated in optically active or racemic forms. It is well known in the art how to prepare optically active forms, such as by resolution of racemic forms (racemates), by asymmetric synthesis, or by synthesis from optically active starting materials. Resolution of the racemates can be accomplished, for example, by conventional methods such as crystallization in the presence of a resolving agent, or chromatography, using, for example a chiral HPLC column. Many geometric isomers of olefins, C ⁇ N double bonds, and the like can also be present in the compounds described herein, and all such stable isomers are contemplated in the present invention.
  • Cis and trans geometric isomers of the compounds of the present invention are described and may be isolated as a mixture of isomers or as separated isomeric forms. All chiral (enantiomeric and diastereomeric), and racemic forms, as well as all geometric isomeric forms of a structure are intended, unless the specific stereochemistry or isomeric form is specifically indicated.
  • any variable occurs more than one time in any constituent or formula for a compound, its definition at each occurrence is independent of its definition at every other occurrence.
  • R* indicates any variable group such as R
  • said group may optionally be substituted with up to three R* groups and R* at each occurrence is selected independently from the definition of R*.
  • combinations of substituents and/or variables are permissible only if such combinations result in stable compounds.
  • any group such as an aryl group, heteroaryl group, carbocyclic group, heterocyclic group, or monocylic or bicyclic ring is said to be “optionally substituted by one or more substituents” that group may contain 0 or from 1 to the maximum number of substituents allowable without exceeding the valency of the atoms of the substituted group.
  • substituents such groups are substituted with 0 or from 1 to 4 substituents, and more preferably such groups are substituted with 0 or from 1 to 3 substituents.
  • such groups are not substituted with more that one oxo substituent.
  • a dash “-” that is not between two letters or symbols is used to indicate a point of attachment for a substituent.
  • —C( ⁇ O)NH 2 is attached through the carbon atom.
  • alkyl is intended to include both branched and straight-chain aliphatic hydrocarbon groups, having the specified number of carbon atoms. Alkyl groups of 2 or more carbon atoms may contain double or triple bonds. Examples of alkyl include, but are not limited to, methyl, ethyl, n-propyl, i-propyl, n-butyl, s-butyl, t-butyl, n-pentyl, and s-pentyl. Preferred alkyl groups are C 1 -C 6 alkyl groups. “C 1 -C 6 alkyl” indicates alkyl groups having from 1 to about 6 carbon atoms.
  • alkoxy represents an alkyl group as defined above with the indicated number of carbon atoms attached through an oxygen bridge.
  • alkoxy include, but are not limited to, methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy, 2-butoxy, t-butoxy, n-pentoxy, 2-pentoxy, 3-pentoxy, isopentoxy, neopentoxy, n-hexoxy, 2-hexoxy, 3-hexoxy, and 3-methylpentoxy.
  • C 1 -C 6 alkoxy indicates alkoxy groups having from 1 to about 6 carbon atoms.
  • Alkenyl is intended to include hydrocarbon chains of either a straight or branched configuration comprising one or more unsaturated carbon-carbon bonds which may occur in any stable point along the chain, such as ethenyl and propenyl. Alkenyl groups typically will have 2 to about 8 carbon atoms, more typically 2 to about 6 carbon atoms.
  • Alkynyl is intended to include hydrocarbon chains of either a straight or branched configuration comprising one or more triple carbon-carbon bonds which may occur in any stable point along the chain, such as ethynyl and propynyl. Alkynyl groups typically will have 2 to about 8 carbon atoms, more typically 2 to about 6 carbon atoms.
  • Aryl refers to aromatic groups having 1 or more rings, wherein the members of the aromatic ring or rings are carbon. When indicated such groups may be substituted. Preferred aryl groups include optionally substituted phenyl and optionally substituted naphthyl.
  • Cycloalkyl is intended to include saturated ring groups, having the specified number of carbon atoms, such as cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl. Cycloalkyl groups typically will have 3 to about 8 ring members.
  • (cycloalkyl)alkyl Cycloalkyl and alkyl are as defined above and the point of attachment is on the alkyl group. This term encompasses, but is not limited to, cyclopropylmethyl, cyclohexylmethyl, cyclohexylmethyl.
  • haloalkyl include, but are not limited to, trifluoromethyl, difluoromethyl, trichloromethyl, pentafluoroethyl, and pentachloroethyl.
  • haloalkoxy indicates a haloalkyl group as defined above with the indicated number of carbon atoms attached through an oxygen bridge.
  • haloalkoxy groups include, but are not limited to, trifluoromethoxy and trichloromethoxy.
  • heteroaryl is intended to mean a stable 5- to 7-membered monocyclic or 7- to 10-membered bicyclic heterocyclic aromatic ring which consists of carbon atoms and from 1 to 4 heteroatoms independently selected from the group consisting of N, O and S. It is preferred that the total number of S and O atoms in the heteroaryl group is not more than 1.
  • heteroaryl groups include, but are not limited to, pyrimidinyl, pyridyl, quinolinyl, benzothienyl, indolyl, pryidazinyl, pyazinyl, isoindolyl, isoquinolyl, quinazolinyl, quinoxalinyl, phthalazinyl, imidazolyl, isoxazolyl, pyrazolyl, oxazolyl, thienyl, thiazolyl, indolizinyl, indazolyl, benzothiazolyl, benzimidazolyl, benzofuranyl, benzoisoxolyl, dihydro-benzodioxinyl, furanyl, pyrrolyl, oxadiazolyl, thiadiazolyl, triazolyl, tetrazolyl, oxazolopyridinyl, imidazopyridinyl, isothi
  • Preferred heteroaryl groups include imidazolyl, pyrrolyl, pyridyl, thiazolyl, pyrazolyl, thiazolyl, isoxazolyl, triazolyl, tetrazolyl, oxadiazolyl, pyrimidinyl, and oxazolyl.
  • heterocycloalkyl is intended to include saturated ring groups having at least 1 heteroatom.
  • Heterocycloalkyl groups typically include 3 to 8 ring atoms, preferably 5 to 7 ring atoms.
  • Heterocycloalkyl groups typically have from 1 to 3 heteroatoms selected from N, S, and O with remaining ring atoms being carbon. Preferably not more than one S atom and one O atom is present in a heterocycloalkyl group.
  • Preferred heterocycloalkyl groups include morpholinyl, piperidinyl, piperazinyl, thiomorpholinyl, and pyrrolidinyl.
  • monocyclic or bicyclic ring refers to saturated, partially unsaturated, or aromatic rings or ring systems, which optionally contain from 1 to 4 heteroatoms independently chosen from N, S, and O with remaining ring members being carbon.
  • Preferred monocyclic and bicyclic rings are saturated and partially unsaturated rings or ring systems.
  • oxo indicates a carbonyl group. When an oxo group appears as a substituent the allowed valence of the substituted position is not exceeded.
  • the compounds of general Formulas I may be administered orally, topically, parenterally, by inhalation or spray or rectally in dosage unit formulations containing conventional non-toxic pharmaceutically acceptable carriers, adjuvants and vehicles. Oral administration in the form of a pill, capsule, elixir, syrup, lozenge, troche, or the like is particularly preferred.
  • parenteral as used herein includes subcutaneous injections, intradermal, intravascular (e.g., intravenous), intramuscular, spinal, intrathecal injection or like injection or infusion techniques.
  • a pharmaceutical formulation comprising a compound of general Formula I and a pharmaceutically acceptable carrier.
  • One or more compounds of general Formula I may be present in association with one or more non-toxic pharmaceutically acceptable carriers and/or diluents and/or adjuvants and if desired other active ingredients.
  • the pharmaceutical compositions containing compounds of general Formula I may be in a form suitable for oral use, for example, as tablets, troches, lozenges, aqueous or oily suspensions, dispersible powders or granules, emulsion, hard or soft capsules, or syrups or elixirs.
  • compositions intended for oral use may be prepared according to any method known to the art for the manufacture of pharmaceutical compositions and such compositions may contain one or more agents selected from the group consisting of sweetening agents, flavoring agents, coloring agents and preserving agents in order to provide pharmaceutically elegant and palatable preparations.
  • Tablets contain the active ingredient in admixture with non-toxic pharmaceutically acceptable excipients that are suitable for the manufacture of tablets.
  • excipients may be for example, inert diluents, such as calcium carbonate, sodium carbonate, lactose, calcium phosphate or sodium phosphate; granulating and disintegrating agents, for example, corn starch, or alginic acid; binding agents, for example starch, gelatin or acacia, and lubricating agents, for example magnesium stearate, stearic acid or talc.
  • the tablets may be uncoated or they may be coated by known techniques to delay disintegration and absorption in the gastrointestinal tract and thereby provide a sustained action over a longer period.
  • a time delay material such as glyceryl monosterate or glyceryl distearate may be employed.
  • Formulations for oral use may also be presented as hard gelatin capsules wherein the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or as soft gelatin capsules wherein the active ingredient is mixed with water or an oil medium, for example peanut oil, liquid paraffin or olive oil.
  • an inert solid diluent for example, calcium carbonate, calcium phosphate or kaolin
  • water or an oil medium for example peanut oil, liquid paraffin or olive oil.
  • Aqueous suspensions contain the active materials in admixture with excipients suitable for the manufacture of aqueous suspensions.
  • excipients are suspending agents, for example sodium carboxymethylcellulose, methylcellulose, hydropropylmethylcellulose, sodium alginate, polyvinylpyrrolidone, gum tragacanth and gum acacia; dispersing or wetting agents may be a naturally-occurring phosphatide, for example, lecithin, or condensation products of an alkylene oxide with fatty acids, for example polyoxyethylene stearate, or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethyleneoxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and a hexitol such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol anhydrides, for example polyethylene sorbitan monooleate
  • the aqueous suspensions may also contain one or more preservatives, for example ethyl, or n-propyl p-hydroxybenzoate, one or more coloring agents, one or more flavoring agents, and one or more sweetening agents, such as sucrose or saccharin.
  • preservatives for example ethyl, or n-propyl p-hydroxybenzoate
  • coloring agents for example ethyl, or n-propyl p-hydroxybenzoate
  • flavoring agents for example ethyl, or n-propyl p-hydroxybenzoate
  • sweetening agents such as sucrose or saccharin.
  • Oily suspensions may be formulated by suspending the active ingredients in a vegetable oil, for example arachis oil, olive oil, sesame oil or coconut oil, or in a mineral oil such as liquid paraffin.
  • the oily suspensions may contain a thickening agent, for example beeswax, hard paraffin or cetyl alcohol. Sweetening agents such as those set forth above, and flavoring agents may be added to provide palatable oral preparations. These compositions may be preserved by the addition of an anti-oxidant such as ascorbic acid.
  • Dispersible powders and granules suitable for preparation of an aqueous suspension by the addition of water provide the active ingredient in admixture with a dispersing or wetting agent, suspending agent and one or more preservatives.
  • a dispersing or wetting agent e.g., glycerol, glycerol, glycerol, glycerol, glycerol, glycerol, glycerin, glycerin, glycerin, glycerin, glycerin, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, glycerol, glycerol, glycerol, glycerol, glycerol, glycerol, glycerol, glycerol, glycerol
  • compositions of the invention may also be in the form of oil-in-water emulsions.
  • the oily phase may be a vegetable oil, for example olive oil or arachis oil, or a mineral oil, for example liquid paraffin or mixtures of these.
  • Suitable emulsifying agents may be naturally-occurring gums, for example gum acacia or gum tragacanth, naturally-occurring phosphatides, for example soy bean, lecithin, and esters or partial esters derived from fatty acids and hexitol, anhydrides, for example sorbitan monoleate, and condensation products of the said partial esters with ethylene oxide, for example polyoxyethylene sorbitan monoleate.
  • the emulsions may also contain sweetening and flavoring agents.
  • Syrups and elixirs may be formulated with sweetening agents, for example glycerol, propylene glycol, sorbitol or sucrose. Such formulations may also contain a demulcent, a preservative and flavoring and coloring agents.
  • the pharmaceutical compositions may be in the form of a sterile injectable aqueous or oleaginous suspension. This suspension may be formulated according to the known art using those suitable dispersing or wetting agents and suspending agents which have been mentioned above.
  • the sterile injectable preparation may also be sterile injectable solution or suspension in a non-toxic parentally acceptable diluent or solvent, for example as a solution in 1,3-butanediol.
  • Suitable vehicles and solvents that may be employed are water, Ringer's solution and isotonic sodium chloride solution.
  • sterile, fixed oils are conventionally employed as a solvent or suspending medium.
  • any bland fixed oil may be employed including synthetic mono- or diglycerides.
  • fatty acids such as oleic acid find use in the preparation of injectables.
  • the compounds of general Formulas I may also be administered in the form of suppositories, e.g., for rectal administration of the drug.
  • suppositories e.g., for rectal administration of the drug.
  • These compositions can be prepared by mixing the drug with a suitable non-irritating excipient that is solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum to release the drug.
  • suitable non-irritating excipient that is solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum to release the drug.
  • Such materials are cocoa butter and polyethylene glycols.
  • Compounds of general Formulas I may be administered parenterally in a sterile medium.
  • the drug depending on the vehicle and concentration used, can either be suspended or dissolved in the vehicle.
  • adjuvants such as local anesthetics, preservatives and buffering agents can be dissolved in the vehicle.
  • the composition may also be added to the animal feed or drinking water. It will be convenient to formulate these animal feed and drinking water compositions so that the animal takes in an appropriate quantity of the composition along with its diet. It will also be convenient to present the composition as a premix for addition to the feed or drinking water.
  • Dosage levels of the order of from about 0.1 mg to about 140 mg per kilogram of body weight per day are useful in the treatment of the above-indicated conditions (about 0.5 mg to about 7 g per patient per day).
  • the amount of active ingredient that may be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated and the particular mode of administration. Dosage unit forms will generally contain between from about 1 mg to about 500 mg of an active ingredient.
  • Frequency of dosage may also vary depending on the compound used and the particular disease treated. However, for treatment of most disorders, a dosage regimen of 4 times daily or less is preferred. For the treatment of anxiety, depression, or cognitive impairment a dosage regimen of 1 or 2 times daily is particularly preferred. For the treatment of sleep disorders a single dose that rapidly reaches effective concentrations is desirable.
  • the specific dose level for any particular patient will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, and rate of excretion, drug combination and the severity of the particular disease undergoing therapy.
  • Preferred compounds of the invention will have certain pharmacological properties. Such properties include, but are not limited to high solubility (preferably 500 ng/ml or more) in aqueous solutions, oral bioavailability, low toxicity, low serum protein binding, lack of clinically relevant EKG effects, and desirable in vitro and in vivo half-lifes. Penetration of the blood brain barrier for compounds used to treat CNS disorders is necessary, while low brain levels of compounds used to treat periphereal disorders are often preferred.
  • Assays may be used to predict these desirable pharmacological properties. Assays used to predict bioavailability include transport across human intestinal cell monolayers, including Caco-2 cell monolayers. Toxicity to cultured hepatocycles may be used to predict compound toxicity. Penetration of the blood brain barrier of a compound in humans may be predicted from the brain levels of the compound in laboratory animals given the compound intravenously.
  • Serum protein binding may be predicted from albumin binding assays. Such assays are described in a review by Oravcová, et al. (Journal of Chromatography B (1996) volume 677, pages 1-27).
  • Compound half-life is inversely proportional to the frequency of dosage of a compound.
  • In vitro half-lifes of compounds may be predicted from assays of microsomal half-life as described by Kuhnz and Gieschen (Drug Metabolism and Disposition, (1998) volume 26, pages 1120-1127).
  • Scheme I illustrates a route to selected compounds of Formula 6 via coupling of chloromethyl compounds 4 and aryl imidazoles 5.
  • aryl and heteroaryl halides of formula 1 are reacted with appropriate amines in the presence of base to obtain amino adducts of formula 2.
  • reduction of the nitro group in compounds of formula 2 yields diamines 3.
  • diamines of formula 3 are reacted with 2-chloro-acetimidic acid methyl ester hydrochloride or a similar electrophile such as 2-chloro-1,1,1-trimethoxy-ethane or chloroacetic acid anhydride.
  • Step 4 chloromethyl compounds of formula 4 are reacted with aryl and heteroaryl imidazoles of formula 5 in the presence of base and solvent to obtain compounds of formula 6.
  • aryl and heteroaryl imidazoles of formula 5 in the presence of base and solvent to obtain compounds of formula 6.
  • a stronger or weaker base may be selected to facilitate the reaction in Step 4.
  • Scheme II illustrates the synthesis of compounds of formula 10 from diamines 3.
  • reaction of malonyl dichloride with ethyl vinyl ether provides 7.
  • Step 2 treatment of 7 with triethyl orthoformate in the presence of acid yields 8.
  • Compound 8 is reacted in Step 3 with a variety of aryl and heteroaryl hydrazines to obtain compounds of formula 9 as a mixture with the undesired regioisomer.
  • compounds of formula 9 can be hydrolyzed to the corresponding acids and coupled with compounds of formula 3 to obtain, following cyclization in refluxing acetic acid, compounds of formula 10.
  • compounds of formula 9 may also be directly coupled to compounds of formula 3 in the presence of trimethylaluminum.
  • cyclization may occur without need for heating in acetic acid as described in Step 5.
  • Scheme III illustrates a method for preparing compounds of formula 21 and 22.
  • Step 1 encompasses hydrolysis of compounds of formula 11 to the corresponding acids followed by dimerization in the presence of a suitable coupling reagent such as 1,1′-carbonyldiimidazole to form compounds of formula 12.
  • Step 2 deacylation of compounds of formula 12 is accomplished by heating with concentrated sulfuric acid to obtain compounds of formula 13. Heating of compounds of formula 13 with ammonium hydroxide in Step 3 results in formation of compounds of formula 14.
  • Nitration of compounds of formula 14 in Step 4 is accomplished using nitric acid to obtain compounds of formula 15.
  • Compounds of formula 15 are converted to the corresponding chlorides 16 in Step 5 by heating with phosphorous oxychloride.
  • Step 6 chlorides 16 are reacted with ammonia followed by heating with phosphorous oxychloride to obtain 2-chloropyridines 17, which are subsequently reduced to diamines 18 in Step 7.
  • diamines 18 are reacted with esters of formula 19 in the presence of trimethylaluminum followed by heating in acetic acid to obtain compounds of formula 20.
  • cyclization may occur without need for heating in acetic acid.
  • Step 9 illustrates alkylation of compounds of formula 20 with ethyl iodide in the presence of base to obtain a mixture of compounds of formula 21 and 22. Those skilled in the art will realize that alternate alkylating agents may be employed to obtain similar compounds bearing different R 5 groups.
  • Scheme IV illustrates a variation of Scheme III for specifically preparing compounds of formula 22.
  • compounds of formula 16 are reacted with ethyl amine to form the amino adducts which are subsequently converted to 2-chloropyridines 23 by reaction with phosphorous oxychloride.
  • Those skilled in the art will realize that numerous other suitable amines of formula R 5 NH 2 may be employed in Step 1 to yield other variants of Formula I.
  • Steps 2 and 3 hydrogenation of compounds of formula 23 to diamines of formula 24 followed by trimethylaluminum-facilitated coupling and cyclization in acetic acid provides compounds of formula 22.
  • cyclization may occur without need for heating in acetic acid
  • Scheme V illustrates a route employing a protecting group strategy for preparing pyrazole compounds of formula 29.
  • pyrazole 25 is reacted with di-tert-butyldicarbonate in the presence of 4-dimethylaminopyridine to obtain 26.
  • Reaction with glyoxal and ammonium hydroxide provides 27.
  • Reaction of 27 with chloromethyl compounds of formula 4 in the presence of base provides compounds of formula 28.
  • Deprotection of compounds of formula 28 with acid in Step 4 provides pyrazoles of formula 29.
  • Scheme VI provides a route for preparing thiazole compounds of formula 33.
  • Step 1 involves bromination of ketoesters of formula 30 to form ⁇ -bromoketones of formula 31.
  • compounds of formula 31 are reacted with thioformamide to obtain thiazoles of formula 32.
  • Condensation of 32 with 3 in Step 3 in the presence of trimethylaluminum provides compounds of formula 33.
  • the product mixture may require heating in a suitable solvent such as acetic acid to enhance formation of 33.
  • Scheme VII provides routes to several heterocyclic systems via common intermediate 35.
  • Step X compounds of formula 34 are reacted with compounds of formula 4 at low temperature in the presence of a suitable base such as lithium diisopropylamide to form compounds of formula 35.
  • R ⁇ n 1 illustrates conversion of ketones of formula 35 to isoxazole derivatives of formula 36 by reaction of compounds of formula 35 with tris(dimethylamino)methane followed by treatment with hydroxylamine.
  • R ⁇ n 2 compounds of formula 35 are reacted with tris(dimethylamino)methane followed by treatment with hydrazine acetate to obtain pyrazoles 37.
  • R ⁇ n 3 and 4 compounds of formula 35 are brominated to form ⁇ -bromoketones 38 that are subsequently reacted with thioformamide to obtain thiazoles of formula 39.
  • R ⁇ n 5 illustrates the synthesis of pyrazoles of formulas 40 and 41 by reaction of compounds of formula 35 with tris(dimethylamino)methane followed by treatment with methyl hydrazine.
  • Scheme VIII provides a route for preparation of tetrazoles of formula 44.
  • aryl and heteroaryl tetrazoles of formula 42 are heated with bis(tributyltin) oxide to form stannanes of formula 43.
  • Step 2 heating compounds of formula 43 with compounds of formula 4 in a suitable solvent such as toluene gives compounds of formula 44.
  • Scheme IX illustrates the synthesis of triazoles of formula 50.
  • aryl and heteroaryl hydrazines are reacted with 1,3,5-triazine 45 to obtain triazoles of formula 46.
  • heating compounds of formula 46 with formaldehyde provides alcohols of formula 47.
  • alcohols of formula 47 are converted to the corresponding chlorides by treatment with thionyl chloride.
  • the chlorides are subsequently converted to nitrites 48 by the action of tetraethyl ammonium cyanide.
  • Cyanides 48 are hydrolyzed in Step 4 to carboxylic acids 49.
  • carboxylic acids of formula 49 are coupled with diamines 3 in the presence of EDCI [1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride] or other suitable coupling reagents followed by heating in acetic acid to complete cyclization of the intermediate amino amides to compounds of formula 50.
  • EDCI 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride
  • Scheme X illustrates two routes for the synthesis of imidazoles of formula 52, which are intermediates in the synthesis of selected compounds of Formula I.
  • aryl and heteroaryl aldehydes are treated with glyoxal and ammonium hydroxide to form imidazoles of formula 52.
  • imidazole 53 is treated with butyl lithium followed by tri-n-butyltin chloride to obtain compounds of formula 54, which must be handled with care to avoid decomposition.
  • compounds of formula 54 are utilized in palladium cross-coupling reactions with aryl and heteroaryl halides to obtain compounds of formula 55. Subsequent treatment of compounds of formula 55 with acid in Step 3′ provides compounds of formula 52.
  • the starting materials and various intermediates may be obtained from commercial sources, prepared from commercially available organic compounds, or prepared using known synthetic methods. Representative examples of methods suitable for preparing intermediates of the invention are set forth below.
  • chloroacetylchloride rather than ethyl chloroacetimidate can by used: To a solution of 3-amino-4-n-propylaminobenzonitrile (5.15 g, 29.4 mmol) and triethylamine (4.51 mL) in ethyl acetate (52 mL) at room temperature, chloroacetyl chloride (2.57 mL) is added slowly. After stirring the reaction mixture for 30 minutes at room temperature, acetic acid (5 mL) is added and the reaction mixture heated to reflux. After heating for 20 h, the reaction mixture is cooled to room temperature and diluted with water (50 mL).
  • the organic solution is washed twice with 1.0 M sodium hydroxide (2 ⁇ 50 mL), then washed with an aqueous solution of 0.25 M KH 2 PO 4 (50 mL), followed by brine (50 mL).
  • the organic layer is dried (sodium sulfate), concentrated in vacuo, and the solid recrystallized by heating in ethyl acetate (20 mL), adding hexane (40 mL) and cooling to room temperature with stirring to afford 1-n-Propyl-2-chloromethyl-5-cyanobenzimidazole as brown crystals.
  • Trimethylaluminum (0.54 mL of 2.0M in toluene, 1.09 mmol) is added dropwise to a solution of 3-Amino-4-propylaminobenzonitrile (152 mg; 0.87 mmol) in dichloromethane (10 mL), and the mixture was stirred at room temperature for 1 hr.
  • a solution of [2-(2-Fluoropyridin-6-yl)-imidazol-1-yl]acetic acid methyl ester (102 mg; 0.43 mmol) in dichloromethane (5 mL) is added all at once, and the mixture heated at reflux for 16 hr.
  • the brown solution is cooled to room temperature and treated dropwise with methanol (1 mL) then water (2 mL) and stirred at room temperature for 15 min.
  • Anhydrous sodium sulfate is added until the gel becomes solid, the mixture is diluted with dichloromethane (100 mL) and filtered through celite. The filtrate is concentrated to give a brown oil which is dissolved in acetic acid (7 mL) and heated at 100° for 72 hr. The mixture is cooled to room temperature and concentrated.
  • 1-(3-Fluorophenyl)-5-hydroxymethyl-1,2,4-triazole (1.7 g) is treated with thionyl chloride (10 mL) in dichloromethane (20 mL) at room temperature overnight. The solvent is then removed. To the residue is added acetonitrile (20 mL), tetraethylammonium cyanide (2.75 g, 17.6 mmol), and triethylamine (2.5 mL, 17.6 mmol). The mixture is stirred at room temperature for 2 h.
  • aqueous layer is adjusted to pH 10 with 3 N NaOH, then extracted with ethyl acetate (3 ⁇ ), and the combined organic extracts washed with water (2 ⁇ ) then saturated aqueous sodium chloride, dried (MgSO 4 ) and concentrated to give 1-Ethyl-2- ⁇ [2-(2,5-difluorophenyl)-1H-imidazol-1-yl]methyl ⁇ -5-amino-1H-benzimidazole.
  • reaction mixture is concentrated to 1 ⁇ 3 volume, water (10 mL) is added and then mixture is then extracted with ethyl acetate (3 ⁇ ). The combined organic layers are washed with aqueous sodium bicarbonate (2 ⁇ ), then washed with saturated aqueous sodium chloride, dried (MgSO 4 ), and concentrated to give 641 mg of crude 1-Ethyl-2- ⁇ [2-(2,5-difluorophenyl)-1H-imidazol-1-yl]methyl ⁇ -5-azido-1H-benzimidazole.
  • Oxalyl chloride (2.5 eq of 2M in dichloromethane) is added dropwise to a solution of 1-ethyl-2- ⁇ [2-(2,5-difluorophenyl)-pyrazol-3-yl]methyl ⁇ -1H-benzimidazole-5-carboxylate (368 mg) in DMF (5 drops) and dichlormethane (30 mL) at 0° C. The mixture is stirred at 0° C. for 0.5 h, then at room temperature for 1 h. The solution is concentrated, the residue taken up in DMF (30 ml), excess propargylamine added, and the mixture stirred for 6 h.
  • 2-Fluoro-6-methylpyridine (14.4 g, 0.13 mol) and tert-butoxybis(dimethylamino)methane (Bredereck's reagent; 34.9 g, 0.20 mol) are heated at 140° C. for 24 h.
  • the reaction is cooled and diluted with THF (100 mL).
  • Sodium periodate (75 g) in water (400 mL) is added at 0-5° C., and the reaction mixture is then stirred for 24 h at room temperature.
  • the precipitate is filtered through celite, and the filtrate extracted 5 ⁇ with diethyl ether.
  • the combined ether layers are washed with water, brine, and dried (MgSO 4 ). Most of the solvent is removed by concentration at 0° C. (by keeping ice in the bath) to provide 2-Fluoropyridine-6-carboxaldehyde.
  • 2-Fluoropyridine-6-carboxaldehyde can also be prepared as follows: To a solution of diisopropylamine (6.54 mL, 1.2 equiv) in 30 mL of THF at 0° C. a solution of n-butyllithium (17.1 mL, 2.5M in hexanes) is added dropwise. Stirring is continued for 15 minutes at 0° C., the reaction is then cooled to ⁇ 78° C. 2-Fluoro-6-methylpyridine (4.00 mL, 38.9 mmol) is added dropwise to the cold solution. The reaction mixture is stirred at ⁇ 78° C.
  • the organic layer is washed with brine (20 mL), diluted with hexanes (15 mL), passed through a plug of silica gel (1 ⁇ 4 inch deep ⁇ 11 ⁇ 4 inch diameter), and the plug washed with more 2:1 ethyl acetate/hexanes (20 mL).
  • the combined eluents are concentrated in vacuo to yield crude 2-Fluoro-6-(1H-imidazol-2-yl)-pyridine.
  • Aqueous hydrogen peroxide (30 mL of 30%) is added dropwise to an ice cold mixture of 3-chloropyridine (6.0 g, 53 mmol) in acetic anhydride (30 mL). The mixture is allowed to stir at ambient temperature for 24 h, water is then added and the mixture concentrated. The residue is taken up in concentrated sulfuric acid (10 mL) and fuming sulfuric acid (5 mL), and then cooled to 0° C. Concentrated nitric acid (24 mL) is added slowly, and the ice bath removed. The reaction is then heated at reflux for 2 h, cooled, and poured into ice water. Ammonium bicarbonate is added carefully until pH 8 is achieved, the solution is then extracted with dichloromethane.
  • n-BuLi (12.0 mL, 19.2 mmol) is slowly added to a solution of 1-ethoxymethyl-1H-imidazole [available via the procedure outlined in Tang, C. C.; Davalian, D.; Huang, P.; Breslow, R. J. Am. Chem. Soc. 1978, 100, 3918] (2.20 g, 17.4 mmol) in THF (30 mL) at ⁇ 78° C. under N 2 . The reaction mixture is stirred at ⁇ 78° C. for 20 min. whereupon tributyltin chloride (5.7 mL, 20.9 mmol) is slowly added. The reaction mixture is stirred at ⁇ 78° C. for 10 min.
  • the compound of this example can be prepared essentially according to the procedures described in Example 21.
  • Trimethylaluminum (11 ml of 2.0M in toluene, 22.4 mmol) is added dropwise to a solution of N-propyl-pyridine-3,4-diamine (1.70 g, 11.2 mmol) in dichloromethane (50 ml), and the mixture is stirred for 30 minutes at room temperature.
  • a solution of [2-(6-fluoro-pyridin-2-yl)-imidazol-1-yl]-acetic acid methyl ester (1.32 g, 5.61 mmol) in dichloromethane (10 ml)
  • the mixture is heated at reflux for 18 hours.
  • the resulting brown mixture is poured into ice-water and filtered through celite.
  • Aqueous 30% H 2 O 2 (60 mL) is added dropwise to a magnetically stirred solution of 3-chloro-pyridine (12 g, 105 mmol) in acetic anhydride (60 mL) under cold conditions (0 to 10° C.). The resulting mixture is allowed to warm up to room temperature slowly and then stirred overnight at room temperature. The reaction mixture is quenched with water (50 mL), diluted with toluene and concentrated to obtain the crude N-oxide as an oil in near quantitative yield.
  • H 2 SO 4 25 mL
  • HNO 3 fluoride, 90%, 60 mL
  • the resulting mixture is then heated at 120° C. for 4 h with stirring, cooled, poured into ice-cold water, and extracted with CHCl 3 .
  • the mixture is further diluted with 0.5 N aq NaOH (10 mL) and then stirred vigorously for 30 min.
  • the mixture is then extracted thrice with CH 2 Cl 2 containing some ( ⁇ 5%) MeOH, and the combined extracts are dried over K 2 CO 3 and concentrated.
  • the mixture is diluted with CH 2 Cl 2 and stirred vigorously for 15 min. It is then filtered through a pad of Celite, and the filtrate concentrated.
  • the crude material is partially purified by preparative thin layer chromatography, developing with 15:1 CHCl 3 -MeOH (+1% Et 3 N).
  • the band containing the desired N 3 -ethyl-6-propyl-pyridine-3,4-diamine does not separate well from the approximately 10% of the slightly more polar, unreacted N-(4-amino-6-propyl-pyridin-3-yl)-acetamide. Both bands are collected, and the mixture is carried into the next reaction.
  • N 3 -ethyl-6-propyl-pyridine-3,4-diamine (82 mg, ⁇ 0.45 mmol, contaminated with ⁇ 10% of N-(4-amino-6-propyl-pyridin-3-yl)-acetamide) in 1,2-dichloroethane (4 mL) at room temperature under N 2 is treated with 2.0 M AlMe 3 (0.69 mL, 1.37 mmol).
  • 2-Chloromethyl-3-ethyl-6-trifluoromethyl-3H-imidazo[4,5-c]pyridine is made from N 3 -Ethyl 6-Trifluoromethyl-pyridine-3,4-diamine following the procedure described previously for the preparation of 2-Chloromethyl-1-ethyl-6-trifluoromethyl-1H-imidazo[4,5-c]pyridine.
  • 3-ethyl-2-[(2-pyrimidin-2-yl-1H-imidazol-1-yl)methyl]-3H-imidazo[4,5-c]pyridine-4-carbonitrile is obtained from 2-chloromethyl-3-ethyl-3H-imidazo[4,5-c]pyridine-4-carbonitrile by nucleophilic displacement with 2-(1H-Imidazol-2-yl)-pyrimidine, as described previously, followed by usual work up.
  • a mixture of thiazol-2-ylhydrazine (2.2 g, 19.1 mmol) and 3,5,5-triethoxy-pent-2-enoic acid ethyl ester (70% purity, 7.1 g, 19.1 mmol) is refluxed in acetic acid overnight. Acetic acid is removed. To the residue is added NaHCO 3 (aq.) (40 mL) and ethyl acetate (100 mL). The insoluble material is filtered. The organic layer is dried and solvent removed. The crude is subjected to column separation (hexane/ethyl acetate 3:1) to give (2-thiazol-2-yl-2H-pyrazol-3-yl)-acetic acid ethyl ester as an oil.
  • Trimethylaluminum (2M in toluene) (1.4 mL, 2.85 mmol) is added dropwise under N 2 to a solution of N 4 -Ethyl-pyridine-3,4-diamine (156 mg, 1.14 mmol) in DCM (5 mL). The mixture is stirred at room temperature for 1 hour. A solution of thiazol-2-yl-2H-pyrazol-3-yl)-acetic acid ethyl ester (270 mg, 1.14 mmol) in DCM (2 mL) is added. The mixture is reluxed for 25 hours. On cooling, the reaction is quenched with water added dropwise and DCM (20 mL) is added.
  • Trimethylaluminum (2N in toluene)(0.5 mL, 1 mmol)) is added dropwise under N 2 to a solution of N 4 -Ethyl-pyridine-3,4-diamine (44 mg, 0.32 mmol) in DCM (5 mL). The mixture is stirred at room temperature for 1 hour. A solution of thiazol-2-yl-2H-pyrazol-3-yl)-propionic acid ethyl ester in DCM (2 mL) is added. The mixture is reluxed for 3 days. On cooling, the reaction is quenched with water added dropwise and DCM (20 mL) is added.
  • Phenyltrimethylammonium tribromide (3.4 g, 8.91 mmol) in DCM (20 mL) is added dropwise to a solution of 4-oxo-4-thiazol-2-yl-butyric acid ethyl ester (1.9 g, 8.91 mmol) in DCM (30 mL. The mixture is stirred overnight at room temperature. 5% NaHCO 3 (40 mL) is added to the mixture. The organic layer is separated and the aqueous layer is extracted with DCM (2 ⁇ 40 mL). The combined organic layers are dried and solvent removed. The residue is purified by column (hexanes/ethyl acetate 3:1) to give 3-bromo-4-oxo-4-thiazol-2-yl-butyric acid ethyl ester.
  • Trimethylaluminum (2M in toluene)(2.2 mL, 4.4 mmol) is added dropwise under N 2 yo a solution of N 4 -Ethyl-pyridine-3,4-diamine (237 mg, 1.73 mmol) in DCM (5 mL). The mixture is stirred at room temperature for 1 hour. A solution of [2,4′]bithiazolyl-5′-yl-acetic acid ethyl ester (440 mg, 1.73 mmol) in DCM (2 mL) is added. The mixture is reluxed for 3 days. On cooling, the reaction is quenched with water added dropwise and DCM (40 mL) is added.
  • a mixture of 3-(5-Bromo-1-ethyl-1H-benzoimidazol-2-yl)-1-(3-fluoro-phenyl)-propan-1-one (0.1 g, 0.27 mmol) and tris(dimethylamino)methane (0.077 g, 0.54 mmol) is heated at 60° C. in a sealed tube for 6 hours. The volatile material is removed in vacuo. EtOR (5 mL) and methyl hydrazine (1.1 mmol) are added to the residue. The mixture is heated at 120° C. for 2 hours. The solvent is removed. NaHCO 3 (aq.) (10 mL) and DCM (30 mL) are added to the residue.
  • R 5 in compound number (hereinafter “Cmp. #”) 112 is an ethyl group
  • R 5 in compound 134 is a cyclopropylmethyl group
  • W 1 in compound 132 is a 3-chlorophenyl group.
  • LC-MS data is provided for a number of the compounds in tables 1-5.
  • the following HPLC method was used to obtain this data: YMC-pack pro C 18 column, 33 ⁇ 4.6 mm(L ⁇ ID) 5 ⁇ m particle size. 3 min gradient from 5% to 95% B with 0.5 min hold at 95% B.
  • Solvent A 95% H 2 O-5% MeOH-0.05% TFA;
  • Solvent B 95% MeOH-5% H 2 O-0.05% TFA).
  • Flow rate 2.0 ml/min.
  • Injection volume 1 ⁇ l.
  • MS (ES + ) m/e 360 [MH] + .
  • the LC data is given as HPLC retention times.
  • the compounds of the invention are prepared as radiolabeled probes by carrying out their synthesis using precursors comprising at least one atom that is a radioisotope.
  • the radioisotope is preferably selected from of at least one of carbon (preferably 14 C), hydrogen (preferably 3 H), sulfur (preferably 35 S), or iodine (preferably 125 I).
  • Such radiolabeled probes are conveniently synthesized by a radioisotope supplier specializing in custom synthesis of radiolabeled probe compounds. Such suppliers include Amersham Corporation, Arlington Heights, Ill.; Cambridge Isotope Laboratories, Inc.
  • Tritium labeled probe compounds are also conveniently prepared catalytically via platinum-catalyzed exchange in tritiated acetic acid, acid-catalyzed exchange in tritiated trifluoroacetic acid, or heterogeneous-catalyzed exchange with tritium gas. Such preparations are also conveniently carried out as a custom radiolabeling by any of the suppliers listed in the preceding paragraph using the compound of the invention as substrate. In addition, certain precursors may be subjected to tritium-halogen exchange with tritium gas, tritium gas reduction of unsaturated bonds, or reduction using sodium borotritide, as appropriate.
  • Receptor autoradiography (receptor mapping) is carried out in vitro as described by Kuhar in sections 8.1.1 to 8.1.9 of Current Protocols in Pharmacology (1998) John Wiley & Sons, New York, using radiolabeled compounds of the invention prepared as described in the preceding Example.
  • Rat cortical tissue is dissected and homogenized in 25 volumes (w/v) of Buffer A (0.05 M Tris HCl buffer, pH 7.4 at 4° C.).
  • Buffer A 0.05 M Tris HCl buffer, pH 7.4 at 4° C.
  • the tissue homogenate is centrifuged in the cold (4° C.) at 20,000 ⁇ g for 20 minutes.
  • the supernatant is decanted, the pellet rehomogenized in the same volume of buffer, and centrifuged again at 20,000 ⁇ g.
  • the supernatant of this centrifugation step is decanted.
  • the resulting pellet may be stored at ⁇ 20° C. overnight.
  • the pellet is then thawed and resuspended in 25 volumes of Buffer A (original wt/vol), centrifuged at 20,000 ⁇ g and the supernatant decanted. This wash step is repeated once.
  • the pellet is finally resuspended in 50 volumes of Buffer A.
  • Incubations contain 100 ⁇ l of tissue homogenate, 100 ⁇ l of radioligand, (0.5 nM 3 H-Ro15-1788 [ 3 H-Flumazenil], specific activity 80 Ci/mmol), and test compound or control (see below), and are brought to a total volume of 500 ⁇ l with Buffer A. Incubations are carried for 30 minutes at 4° C. and then rapidly filtered through Whatman GFE filters to separate free and bound ligand. Filters are washed twice with fresh Buffer A and counted in a liquid scintillation counter. Nonspecific binding (control) is determined by displacement of 3 H Ro15-1788 with 10 ⁇ M Diazepam (Research Biochemicals International, Natick, Mass.). Data are collected in triplicate, averaged, and percent inhibition of total specific binding (Total Specific Binding Total—Nonspecific) is calculated for each compound.
  • a competition binding curve may obtained with up to 11 points spanning the compound concentration range from 10 ⁇ 12 M to 10 ⁇ 5 M obtained per curve by the method described above for determining percent inhibition.
  • K i values are calculated according the Cheng-Prussof equation. Each of the compounds disclosed in Tables 6-8 was tested in this fashion and each was found to have a K i of ⁇ 4 ⁇ M.
  • Preferred compounds of the invention exhibit K i values of less than 100 nM and more preferred compounds of the invention exhibit K i values of less than 10 nM.
  • the following assay can be used to determine if a compound of the invention acts as an agonist, an antagonist, or an inverse agonist at the benzodiazepine site of the GABA A receptor.
  • Assays are carried out as described in white and Gurley (NeuroReport 6: 1313-1316, 1995) and White, Gurley, Hartnett, Stirling, and Gregory (Receptors and Channels 3: 1-5, 1995) with modifications. Electrophysiological recordings are carried out using the two electrode voltage-clamp technique at a membrane holding potential of ⁇ 70 mV. Xenopus Laevis oocytes are enzymatically isolated and injected with non-polyadenylated cRNA mixed in a ratio of 4:1:4 for ⁇ , ⁇ and ⁇ subunits, respectively. Of the nine combinations of ⁇ , ⁇ and ⁇ subunits described in the White et al.
  • preferred combinations are ⁇ 1 ⁇ 2 ⁇ 2 , ⁇ 2 ⁇ 3 ⁇ 2 , ⁇ 3 ⁇ 3 ⁇ 2 , and ⁇ 5 ⁇ 3 ⁇ 2 .
  • Preferably all of the subunit cRNAs in each combination are human clones or all are rat clones.
  • the sequence of each of these cloned subunits is available from GENBANK, e.g., human ⁇ 1 , GENBANK accession no. X 14766 , human ⁇ 2 , GENBANK accession no. A28100; human ⁇ 3 , GENBANK accession no. A28102; human ⁇ 5 , GENBANK accession no.
  • Test compound efficacy is calculated as a percent-change in current amplitude: 100*((Ic/I) ⁇ 1), where Ic is the GABA evoked current amplitude observed in the presence of test compound and I is the GABA evoked current amplitude observed in the absence of the test compound.
  • test compound for the benzodiazepine site Specificity of a test compound for the benzodiazepine site is determined following completion of a concentration/effect curve. After washing the oocyte sufficiently to remove previously applied test compound, the oocyte is exposed to GABA+1 ⁇ M RO15-1788, followed by exposure to GABA+1 ⁇ M RO15-1788+test compound. Percent change due to addition of compound is calculated as described above. Any percent change observed in the presence of RO15-1788 is subtracted from the percent changes in current amplitude observed in the absence of 1 ⁇ M RO15-1788. These net values are used for the calculation of average efficacy and EC 50 values by standard methods. To evaluate average efficacy and EC 50 values, the concentration/effect data are averaged across cells and fit to the logistic equation.

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Psychiatry (AREA)
  • Pain & Pain Management (AREA)
  • Psychology (AREA)
  • Anesthesiology (AREA)
  • Hospice & Palliative Care (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)

Abstract

This invention relates to benzimidazoles, pyridylimidazoles and related bicyclic heteroaryl compounds, all of which may be described by of Formula I
Figure US20100267698A1-20101021-C00001
The invention is particularly related to such compounds that bind with high selectivity and high affinity to the benzodiazepine site of GABAA receptors. This invention also relates to pharmaceutical compositions comprising such compounds and to the use of such compounds in treatment of certain central nervous system (CNS) diseases. Novel processes for preparing compounds of Formula I are disclosed.
This invention also relates to the use of benzimidazoles, pyridylimidazoles and related bicyclic heteroaryl compounds of Formula I in combination with one or more other CNS agents to potentiate the effects of the other CNS agents. Additionally this invention relates to the use such compounds as probes for the localization of GABAA receptors in tissue sections.

Description

    BACKGROUND OF THE INVENTION
  • 1. Field of the Invention
  • This invention relates to benzimidazole and pyridylimidazole derivatives, and, more specifically, to such derivatives that bind with high selectively and/or high affinity to the benzodiazepine site of GABAA receptors. This invention also relates to pharmaceutical compositions comprising such compounds and to the use of such compounds in the treatment of central nervous system (CNS) diseases.
  • 2. Description of the Related Art
  • The GABAA receptor superfamily represents one of the classes of receptors through which the major inhibitory neurotransmitter, γ-aminobutyric acid, or GABA, acts. Widely, although unequally, distributed throughout the mammalian brain, GABA mediates many of its actions through a complex of proteins called the GABAA receptor, which causes alteration in chloride conductance and membrane polarization. In addition to being the site of neurotransmitter action, a number of drugs including the anxiolytic and sedating benzodiazepines bind to this receptor. The GABAA receptor comprises a chloride channel that generally, but not invariably, opens in response to GABA, allowing chloride to enter the cell. This, in turn, effects a slowing of neuronal activity through hyperpolarization of the cell membrane potential.
  • GABAA receptors are composed of five protein subunits. A number of cDNAs for these GABAA receptor subunits have been cloned and their primary structures determined. While these subunits share a basic motif of 4 membrane-spanning helices, there is sufficient sequence diversity to classify them into several groups. To date at least 6α, 3β, 3γ, 1ε, 1δ and 2ρ subunits have been identified. Native GABAA receptors are typically composed of 2α, 2β, and 1γ. Various lines of evidence (such as message distribution, genome localization and biochemical study results) suggest that the major naturally occurring receptor combinations are α1β2γ2, α2β3γ2, α3β3γ2, and α5β3γ2 (Mohler et al. Neuroch. Res. 1995; 20(S):631-36).
  • The GABAA receptor binding sites for GABA (2 per receptor complex) are formed by amino acids from the α and β subunits. Amino acids from the α and γ subunits together form one benzodiazepine site per receptor. Benzodiazepines exert their pharmacological actions by interacting with the benzodiazepine binding sites associated with the GABAA receptor. In addition to the benzodiazepine site (sometimes referred to as the benzodiazepine or BDZ receptor), the GABAA receptor contains sites of interaction for several other classes of drugs. These include a steroid binding site, a picrotoxin site, and a barbiturate site. The benzodiazepine site of the GABAA receptor is a distinct site on the receptor complex that does not overlap with the site of interaction for other classes of drugs that bind to the receptor or for GABA (see, e.g., Cooper, et al., The Biochemical Basis of Neuropharmacology, 6th ed., 1991, pp. 145-148, Oxford University Press, New York).
  • In a classic allosteric mechanism, the binding of a drug to the benzodiazepine site increases the affinity of the GABA receptor for GABA. Benzodiazepines and related drugs that enhance the ability of GABA to open GABAA receptor channels are known as agonists or partial agonists depending on the level of GABA enhancement. Other classes of drugs, such as β-carboline derivatives, that occupy the same site and negatively modulate the action of GABA are called inverse agonists. A third class of compounds exists which occupy the same site as both the agonists and inverse agonists and yet have little or no effect on GABA activity. These compounds will, however, block the action of agonists or inverse agonists and are thus referred to as GABAA receptor antagonists.
  • The important allosteric modulatory effects of drugs acting at the benzodiazepine site were recognized early, and the distribution of activities at different subtype receptors has been an area of intense pharmacological discovery. Agonists that act at the benzodiazepine site are known to exhibit anxiolytic, sedative, and hypnotic effects, while compounds that act as inverse agonists at this site elicit anxiogenic, cognition enhancing, and proconvulsant effects. While benzodiazepines have enjoyed long pharmaceutical use as anxiolytics, these compounds are known to exhibit a number of unwanted side effects. These may include cognitive impairment, sedation, ataxia, potentiation of ethanol effects, and a tendency for tolerance and drug dependence.
  • GABAA selective ligands may also act to potentiate the effects of certain other CNS active compounds. For example, there is evidence that selective serotonin reuptake inhibitors (SSRIs) may show greater antidepressant activity when used in combination with GABAA selective ligands than when used alone.
    • SUMMARY OF THE INVENTION
  • This invention provides benzimidazole and pyridylimidazole derivatives that bind to the benzodiazepine site of GABAA receptors, including human GABAA receptors. Preferred compounds of the invention bind with high selectivity and/or high affinity to GABAA receptors. Preferred compounds act as agonists, antagonists or inverse agonists of such receptors. As such, they are useful in the treatment of various CNS disorders.
  • The invention provides compounds of Formula I (shown below), and pharmaceutical compositions comprising compounds of Formula I.
  • The invention provides methods for synthesizing compounds of Formula I.
  • The invention further provides methods of treating patients suffering from certain CNS disorders with an effective amount of a compound of the invention. The patient may be a human or other mammal. Treatment of humans, domesticated companion animals (pets) or livestock animals suffering from certain CNS disorders with an effective amount of a compound of the invention is encompassed by the invention.
  • In a separate aspect, the invention provides methods of potentiating the actions of other CNS active compounds. These methods comprise administering an effective amount of a compound of the invention in conjunction with the administration of another CNS active compound.
  • Additionally this invention relates to the use of compounds of Formula I as probes for the localization of GABAA receptors in tissue sections.
  • In a first aspect, the invention provides compounds of Formula I
  • Figure US20100267698A1-20101021-C00002
  • and the pharmaceutically acceptable salts thereof.
  • In this aspect, Z1 is nitrogen or CR1; Z2 is nitrogen or CR2; Z3 is nitrogen or CR3; and Z4 is nitrogen or CR4; provided that no more than two of Z1, Z2, Z3, and Z4 are nitrogen. Further in Formula I,
  • R1, R2, R3, and R4 are independently selected from
  • i) hydrogen, halogen, hydroxy, nitro, cyano, amino, haloalkyl, and haloalkoxy,
  • ii) alkyl, alkoxy, cycloalkyl, alkenyl, alkynyl, (cycloalkyl)alkyl, —NH(R10), —N(R10)(R11), hydroxyalkyl, aminoalkyl, (R10)NHalkyl-, (R10)(R11)Nalkyl-; alkanoyl, alkoxycarbonyl, (heterocycloalkyl)alkyl, alkylsulfonyl, alkylthio, mono- or dialkylaminocarbonyl, heterocycloalkyl, aryl, and heteroaryl, each of which is optionally substituted with 1, 2, 3, or 4 of R20, wherein R10 and R11, are independently selected from the group consisting of alkyl, alkenyl, alkynyl, alkoxy, cycloalkyl, (cycloalkyl)alkyl, aryl, arylalkyl, alkanoyl, and mono and dialkylaminoalkyl; and iii) a group of the formula:
  • Figure US20100267698A1-20101021-C00003
  • where G is a bond, alkyl, —O—, —C(═O)—, or —CH2C(═O)—, and RA is a saturated, partially unsaturated, or aromatic carbocycle, consisting of 1 ring or 2 fused, pendant, or spiro rings, each ring containing 0, 1, or 2 heteroatoms independently chosen from N, S, and O, said saturated, partially unsaturated, or aromatic carbocycle is optionally substituted with 1, 2, 3, or 4 of R20,
  • iv) a group of the formula
  • Figure US20100267698A1-20101021-C00004
  • where J is N, CH, or C-alkyl, and RB and RC are independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, alkoxy, cycloalkyl, (cycloalkyl)alkyl, heterocycloalkyl, aryl, arylalkyl, alkanoyl, heteroaryl, and mono and dialkylaminoalkyl, each of which is optionally substituted by 1 or 2 substituents independently chosen from halogen, hydroxy, cyano, amino, nitro, alkoxy, and alkyl, RB and RC and the atom to which they are attached form a 4- to 10-membered monocyclic or bicyclic ring, which may contain:
      • a) one or more double bonds,
      • b) one or more of oxo, O, S, SO, SO2, or N—RD wherein RD is hydrogen, Ar1, alkyl, cycloalkyl, heterocycloalkyl, or Ar1alkyl; wherein Ar1 is aryl or heteroaryl, each of which is optionally substituted by 1 or 2 substituents independently chosen from halogen, hydroxy, cyano, amino, nitro, alkoxy, and alkyl,
      • c) one or more substituents R20;
  • v) —OC(═O)RE, —C(═O)ORE, —C(═O)NH2, —C(═O)NHRE, —C(═O)NRERF, —S(O)nRE, —S(O)nNH2, —S(O)nNHRE, —S(O)nNRERF, —NHC(═O)RE, —C(═NRE)RF, —HC═N—OH, —HC═N(alkoxy), —HC═N(alkyl), —NREC(═O)RF, —NHS(O)nRE, and —NRES(O)mRF, where m is 0, 1 or 2, and
  • RE and RF are independently selected at each occurrence from alkyl, cycloalkyl, heterocycloalkyl, alkoxy, mono- or dialkylamino, aryl, or heteroaryl each of which is optionally substituted by 1, 2, or 3 of R30.
  • R20, in this aspect of the invention, is independently selected at each occurrence from the group consisting of: halogen; hydroxy; nitro; cyano; amino; alkyl; alkoxy optionally substituted with amino or mono- or dialkylamino; cycloalkyl; cycloalkylalkyl; cycloalkylalkoxy; alkenyl; alkynyl; haloalkyl; oxo; haloalkoxy; mono- and dialkylamino; aminoalkyl; and mono- and dialkylaminoalkyl.
  • R30 is independently selected at each occurrence from halogen, hydroxy, nitro, cyano, amino, alkyl, alkoxy optionally substituted with amino or mono- or dialkylamino, cycloalkyl, cycloalkylalkyl, cycloalkylalkoxy, heterocycloalkyl, alkenyl, alkynyl, haloalkyl, haloalkoxy, oxo, mono- and dialkylamino, aminoalkyl, and mono- and dialkylaminoalkyl.
  • R5 represents hydrogen or haloalkyl; or
  • R5 represents alkyl, cycloalkyl, or (cycloalkyl)alkyl, each of which may contain one or more double or triple bonds, and each of which is optionally substituted with 1, 2, or 3 of R30, or
  • R5 represents aryl, arylalkyl, heteroaryl, or heteroarylalkyl each of which is optionally substituted with 1, 2, or 3 substituents selected from the group consisting of haloalkyl, amino, —NH(R10), —N(R10)(R11), carboxamido, (R10)NHcarbonyl, (R10)(R11)Ncarbonyl, halogen, hydroxy, nitro, cyano, amino, alkyl, alkoxy optionally substituted with amino or mono- or dialkylamino, cycloalkyl, cycloalkylalkyl, cycloalkylalkoxy, heterocycloalkyl, alkenyl, alkynyl, haloalkyl, haloalkoxy, aminoalkyl, and mono- and dialkylaminoalkyl.
  • Q represents —C(R6)(R7) or oxygen, with the proviso that Q is not oxygen when X2 is nitrogen.
  • R6 and R7 independently represent hydrogen, fluorine, or alkyl.
  • The group:
  • Figure US20100267698A1-20101021-C00005
  • represents a 5 to 7 membered heteroaryl or heterocycloalkyl ring containing up to 4 heteroatoms selected from nitrogen, sulfur, and oxygen, said 5 to 7 membered heteroaryl or heterocycloalkyl ring is substituted at each carbon atom by R, and substituted at each nitrogen atom available for substitution by R′.
  • R is independently chosen at each occurrence from hydrogen, halogen, amino, alkyl, alkenyl, alkynyl, alkoxy, cycloalkyl, (cycloalkyl)alkyl, haloalkyl, haloalkoxy, carboxamido, and 3- to 7-membered carbocyclic or heterocyclic groups which are saturated, unsaturated, or aromatic, which may be further substituted with one or more substituents independently selected from halogen, oxo, hydroxy, alkyl, and alkoxy.
  • R′ is independently chosen at each occurrence from alkyl, hydrogen, cycloalkyl, cycloalkyl(alkyl), and 3- to 7-membered carbocyclic or heterocyclic groups which are saturated, unsaturated, or aromatic, which 3- to 7-membered carbocyclic or heterocyclic groups are optionally substituted with one or more substituents independently selected from halogen, oxo, hydroxy, alkyl, and alkoxy.
  • X1 and X2 independently represent nitrogen, carbon or CH.
  • Y is nitrogen, oxygen, carbon, —CH—, —CH2—, or absent.
  • W represents aryl or heteroaryl, wherein the aryl or heteroaryl group is optionally substituted with up to 4 groups independently selected from R30, —CO2H, —C(═O)ORE, —C(═O)NHRE, —C(═O)NRERF, —C(O)RE, and —S(O)mRE, —ORE, where R30 and RE are as defined above and m is 0, 1, or 2.
    • DETAILED DESCRIPTION
  • In addition to the compounds and salts of Formula I, described above, the invention further provides compounds of Formula I wherein
  • R1, R2, R3, and R4 are independently selected from
  • i) hydrogen, halogen, hydroxy, nitro, cyano, amino, halo(C1-C6)alkyl, and halo(C1-C6)alkoxy,
  • ii) (C1-C6)alkyl, (C1-C6)alkoxy, (C3-C8)cycloalkyl, (C2-C6)alkenyl, alkynyl, ((C3-C8)cycloalkyl) (C1-C4)alkyl, —NH(R10), —N(R10)(R11), hydroxy(C1-C6)alkyl, amino(C1-C6)alkyl, (R10)NH(C1-C6)alkyl, (R10)(R11)N(C3-C6)alkyl, (C1-C6)alkanoyl, (C1-C6)alkoxycarbonyl, (C1-C6)alkylsulfonyl, (C1-C6)alkylthio, mono- or di(C1-C6)alkylaminocarbonyl, heterocycloalkyl, (heterocycloalkyl)C1-C4alkyl, aryl, and heteroaryl, each of which is optionally substituted with 1, 2, 3, or 4 of R20, wherein R10 and R11 are independently selected from the group consisting of (C1-C6)alkyl, (C2-C6)alkenyl, (C1-C6)alkoxy, (C3-C8)cycloalkyl, (C3-C8)cycloalkylalkyl, aryl, aryl(C1-C6)alkyl, (C1-C6)alkanoyl, and mono and di(C1-C6)alkylaminoalkyl;
  • iii) a group of the formula:
  • Figure US20100267698A1-20101021-C00006
  • where G is (C1-C6)alkyl, —O—, —C(═O)—, or —CH2C(═O)—, and
  • RA is a saturated, partially unsaturated, or aromatic carbocycle, consisting of 1 ring or 2 fused, pendant, or spiro rings, each ring consisting of from 3 to 8 ring atoms, and each ring containing 0, 1, or 2 heteroatoms independently chosen from N, S, and O; said saturated, partially unsaturated, or aromatic carbocycle is optionally substituted with 1, 2, 3, or 4 of R20,
  • iv) a group of the formula
  • Figure US20100267698A1-20101021-C00007
  • where J is N, CH, or C—(C1-C6)alkyl and RB and RC are independently selected from the group consisting of hydrogen, (C1-C6)alkyl, (C2-C6)alkenyl, (C2-C6)alkynyl, (C1-C6)alkoxy, (C3-C8)cycloalkyl, (C3-C8cycloalkyl) (C1-C4)alkyl, heterocycloalkyl, aryl, aryl(C1-C4)alkyl, (C1-C6)alkanoyl, heteroaryl, and mono and di(C1-C6)alkylamino(C1-C6)alkyl, each of which is optionally substituted by 1 or 2 substituents independently chosen from halogen, hydroxy, cyano, amino, nitro, C1-C6alkoxy, and C1-C6alkyl; or RB and RC and the atom to which they are attached form a 4- to 10-membered monocyclic or bicyclic ring, which may contain
      • a) one or more double bonds
      • b) one or more of oxo, O, S, SO, SO2, and N—RD wherein RD is hydrogen, Ar1, (C1-C6)alkyl, (C3-C8)cycloalkyl, heterocycloalkyl, or Ar1(C1-C5)alkyl; wherein Ar1 is aryl or heteroaryl, each of which is optionally substituted by 1 or 2 substituents independently chosen from halogen, hydroxy, cyano, amino, nitro, C1-C6alkoxy, and C1-C6alkyl;
      • c) one or more substituents R20;
  • v) —OC(═O)RE, —C(═O)ORE, —C(═O)NH2, —C(═O)NHRE, —C(═O)NRERF, —S(O)nRE, —S(O)nNH2, —S(O)nNHRE, —S(O)nNRERF, —NHC(═O)RE, —C(═NRE)RF, —HC═N—OH, —HC═N(C1-C6alkoxy), —HC═N(C1-C6alkyl) —NREC(═O)RF, —NHS(O)RE, and —NRES(O)mRF, where m is 0, 1 or 2, and RE and RF are independently selected at each occurrence from (C1-C6)alkyl, (C3-C8)cycloalkyl, heterocycloalkyl, (C1-C6)alkoxy, mono- and di(C1-C6)alkylamino, aryl, and heteroaryl each of which is optionally substituted by 1, 2, or 3 of R30.
  • R20 is independently selected at each occurrence from the group consisting of halogen; hydroxy; nitro; cyano; amino; (C1-C6)alkyl; (C1-C6)alkoxy optionally substituted with amino or mono- or di(C1-C6)alkylamino; (C3-C8)cycloalkyl; (C3-C8)cycloalkyl(C1-C4)alkyl; (C3-C8)cycloalkyl(C1-C4)alkoxy; (C2-C6)alkenyl; (C2-C6)alkynyl; halo(C1-C6)alkyl; halo(C1-C6)alkoxy; oxo; mono- and di(C1-C6)alkylamino; amino(C1-C6)alkyl; and mono- and di(C1-C6)alkylamino(C1-C6)alkyl.
  • R30 is independently selected at each occurrence from halogen, hydroxy, nitro, cyano, amino, (C1-C6)alkyl, (C1-C6)alkoxy optionally substituted with amino or mono- or di(C1-C6)alkylamino, (C3-C8)cycloalkyl, (C3-C8)cycloalkyl(C1-C4)alkyl, (C3-C8)cycloalkyl(C1-C4)alkoxy, heterocycloalkyl, (C2-C6)alkenyl, (C2-C6)alkynyl, halo(C1-C6)alkyl, halo(C1-C10)alkoxy, oxo, mono- and di(C1-C6)alkylamino, amino(C1-C6)alkyl, and mono- and di(C1-C6)alkylamino(C1-C6)alkyl.
  • R5 represents hydrogen or halo(C1-C6)alkyl; or
  • R5 represents (C1-C6)alkyl, (C3-C8)cycloalkyl, or (C3-C8cycloalkyl) (C1-C4)alkyl, each of which may contain one or more double or triple bonds, and each of which is optionally substituted with 1, 2, or 3 of R30, or
  • R5 represents aryl, aryl(C1-C4)alkyl, heteroaryl, or heteroaryl(C1-C4)alkyl each of which is optionally substituted with 1, 2, or 3 substituents selected from the group consisting of: halo(C1-C6)alkyl, amino, NH(R10), N(R10)(R11), carboxamido, NH(R10)carbonyl, N(R10)(R11)carbonyl, halogen, hydroxy, nitro, cyano, amino, (C1-C6)alkyl, (C1-C8)alkoxy optionally substituted with amino or mono- or di(C1-C10)alkylamino, (C3-C10)cycloalkyl, (C3-C8)cycloalkyl(C1-C4)alkyl, (C3-C10)cycloalkyl(C1-C4)alkoxy, heterocyclo(C1-C4)alkyl, (C2-C6)alkenyl, (C2-C6)alkynyl, halo(C1-C6)alkyl, halo(C1-C6)alkoxy, amino(C1-C6)alkyl, and mono- and di(C1-C10)alkylamino(C1-C6)alkyl;
  • Q represents —C(R6)(R7) or oxygen, with the proviso that Q is not oxygen when X2 is nitrogen;
  • R6 and R7 independently represent hydrogen, fluorine, or C1-C6alkyl; and
  • the group:
  • Figure US20100267698A1-20101021-C00008
  • represents a 5 to 7 membered heteroaryl or heterocycloalkyl ring containing up to 4 heteroatoms selected from nitrogen, sulfur, and oxygen, said 5 to 7 membered heteroaryl or heterocycloalkyl ring is substituted at each carbon atom by R, and is substituted at each nitrogen atom available for substitution by R′;
  • R is independently chosen at each occurrence from hydrogen, halogen, amino, C1-C6alkyl, (C2-C6)alkenyl, (C2-C6)alkynyl, C1-C6alkoxy, (C3-C8)cycloalkyl, (C3-C8cycloalkyl)(C1-C4)alkyl, halo(C1-C6)alkyl, haloalkoxy, carboxamido, and 3- to 7-membered carbocyclic or heterocyclic groups which are saturated, unsaturated, or aromatic, which may be further substituted with one or more substituents independently selected from halogen, oxo, hydroxy, C1-4alkyl, and —O(C1-4alkyl);
  • R′ is independently chosen at each occurrence from hydrogen, C1-C6alkyl, C3-C8cycloalkyl, C3-C8cycloalkyl(C1-C4alkyl), and 3- to 7-membered carbocyclic or heterocyclic groups which are saturated, unsaturated, or aromatic, which 3- to 7-membered carbocyclic or heterocyclic groups are optionally substituted with one or more substituents independently selected from halogen, oxo, hydroxy, C1-4alkyl, and —O(C1-4alkyl); and
  • X1, X2, W, and Y are as defined for Formula I, above.
  • Such compounds will be referred to as compounds of Formula IA.
  • A particular aspect of the invention is directed to compounds and pharmaceutically acceptable salts of Formula II
  • Figure US20100267698A1-20101021-C00009
  • In Formula II, the variables Z1, Z2, Z3, Z4, R5, Q, X1, X2, and W carry the definition set forth for Formula I, or more preferably, for Formula IA;
  • X3 and X4 are independently selected from the group consisting of carbon, CR, N, O, S, NH, and N(C1-C6)alkyl; provided that at least one of X1, X2, X3, and X4 is carbon or CR; and
  • R is independently chosen at each occurrence from hydrogen, halogen, amino, (C1-C6)alkyl, (C1-C6)alkoxy, (C3-C8)cycloalkyl, (C3-C8)cycloalkyl(C1-C6)alkyl, (C2-C6)alkenyl, (C2-C6)alkynyl, halo(C1-C6)alkyl, halo(C1-C6)alkoxy, carboxamido, and 3- to 7-membered carbocyclic or heterocyclic groups which are saturated, unsaturated, or aromatic, which may be further substituted with one or more substituents independently selected from halogen, oxo, hydroxy, C1-4alkyl, and —O(C1-4alkyl).
  • The invention is particularly directed to compounds of Formula I, Formula IA, and Formula II, in which Z1 is CR1, Z2 is CR2, Z3 is CR3, and Z4 is CR4.
  • The invention is also directed to compounds of Formula I, Formula IA, and Formula II, in which one, and only one, of Z1, Z2, Z3, and Z4 is nitrogen.
  • Another particular aspect of the invention provides compounds of Formula I, Formula IA, and Formula II, in which Z1 is CR1, Z4 is CR4, and only one, of Z2 and Z3 is nitrogen.
  • The invention is further directed to compounds of Formula I, Formula IA, and Formula II wherein:
  • i) X2 is carbon; and Q is oxygen;
  • ii) X2 is N; and Q is C(R6)(R7)
  • iii) X2 is carbon; and Q is C(R6)(R7);
  • iv) X1 is carbon; X2 is N; and Q is C(R6)(R7)
  • v) X1 is nitrogen; X2 is carbon; and Q is C(R5) (R7); or
  • wherein
  • vi) Q is C(R6)(R7)
  • For each of i) through vi) preferred compounds are those where Z1 is CR1, Z2 is CR2, Z3 is CR3, and Z4 is CR4. For each of i) through vi) preferred compounds are those in which one, and only one, of Z1, Z2, Z3, and Z4 is nitrogen. For each of i) through vi) compounds in which Z1 is CR1, and only one, of Z2 and Z3 is nitrogen are particularly preferred.
  • In another aspect, the invention provides compounds of Formula III and Formula IV:
  • Figure US20100267698A1-20101021-C00010
  • wherein R, R1, R2, R3, R4, R5, Q, and W carry the definitions set forth for Formula I, or more preferably for Formula IA.
  • Particular compounds of Formula III included in the invention are those wherein Q is C(R6)(R7).
  • Preferred compounds of Formula IV include those where R6 and R7 are hydrogen, methyl or fluoro and the other is ethyl, or where one of R6 and R7 is hydrogen, methyl or fluoro and the other is ethyl.
  • Other compounds of the invention include compounds of Formula III or Formula IV, wherein R is independently selected at each occurrence from the group consisting of:
    • i) hydrogen, halogen, (C1-C6)alkyl, (C3-C8)cycloalkyl, (C3-C8)cycloalkyl(C1-C6)alkyl, (C1-C6)alkoxy, halo(C1-C6)alkyl, halo(C1-C6)alkoxy, and
    • ii) phenyl and pyridyl each of which is optionally substituted with up to 3 substituents independently chosen from halogen, hydroxy, C1-4alkyl, and —O(C1-4alkyl).
    • Q (in Formula III) is C(R6)(R7).
  • In Formula III and IV, R1, R2, R3, and R4 are independently selected from hydrogen, halogen, hydroxy, nitro, cyano, amino, (C1-C6)alkyl, (C1-C6)alkoxy, (C3-C8)cycloalkyl, (C3-C8)cycloalkyl(C1-C6)alkyl, (C2-C6)alkenyl, (C2-C6)alkynyl, heterocycloalkyl, halo(C1-C6)alkyl, halo(C1-C6)alkoxy, mono or di(C1-C6)alkylamino, amino(C1-C6)alkyl, and mono- and di(C1-C6)alkylamino(C1-C6)alkyl;
  • R5 represents hydrogen, (C1-C6)alkyl, (C3-C8)cycloalkyl, (C3-C8)cycloalkyl(C1-C6)alkyl, phenyl, benzyl, thiophenyl, thiazoyl, pyridyl, imidazolyl, pyrazolyl, or pyrimidinyl;
  • R6 and R7 independently represent hydrogen, fluorine, or C1-C6 alkyl; and
  • W represents phenyl, thienyl, thiazoyl, pyridyl, imidazolyl, oxazolyl, triazolyl, tetrazolyl, pyrazolyl, isoxazolyl, or pyrimidinyl, each of which is optionally substituted with up to 4 R30 groups, where R30 carries the definition set forth for Formula I, or more preferably R30 carries the definition set forth for Formula IA.
  • Particularly included in the invention are compounds of Formula III and Formula IV in which wherein R, R1, R2, R3, R4, R5, Q, and W carry the definition set forth for Formula I, or more preferably for Formula IA, and
  • W represents a 6-membered aryl or heteroaryl groups, wherein the 6-membered aryl or heteroaryl group is optionally substituted with up to 4 groups independently selected from R30, —CO2H, —C(═O)ORE, —C(═O)NHRE, —C(═O)NRERF, —C(O)RE, —S(O)mRE, and —ORE or wherein
  • W represents a 5-membered heteroaryl group, wherein the 5-membered heteroaryl group is optionally substituted with up to 4 groups independently selected from R30, —CO2H, —C(═O)ORE, —C(═O)NHRE, —C(═O)NRERF, —C(O)RE, —S(O)nRE, and —ORE.
  • In these embodiments of the invention m is 0, 1, or 2, and RE carries the definition set forth for Formula I, or more preferably RE carries the definition set forth for Formula IA and R30 carries the definition set forth above with respect to Formula IA.
  • In another aspect, the compounds of Formula III or Formula IV are those in which one of R2 or R3 carries the definition set forth for Formula I, or more preferably for Formula IA.
  • In this aspect of the invention,
  • R is independently selected at each occurrence from the group consisting of hydrogen, halogen, and (C1-C2)alkyl;
  • R1, R4, and the other of R2 and R3 are independently selected from hydrogen, halogen, hydroxy, nitro, cyano, amino, (C1-C6)alkyl, (C1-C6)alkoxy, (C3-C8)cycloalkyl, (C3-C8)cycloalkyl(C1-C6)alkyl, halo(C1-C6)alkyl, halo(C1-C6)alkoxy, mono or di(C1-C6)alkylamino, amino(C1-C6)alkyl, and mono- and di(C1-C6)alkylamino(C1-C6)alkyl;
  • R5 represents (C1-C6)alkyl; and
  • Q (in Formula III) is CH2, and R6 and R7 in Formula IV are hydrogen; and
  • W represents phenyl, furanyl, thienyl, thiazoyl, pyridyl, imidazolyl, oxazolyl, triazolyl, tetrazolyl, pyrazolyl, isoxazolyl, pyrimidinyl, benzimidazolyl, quinolinyl, isoquinolinyl each of which is optionally substituted with up to 4 R30 groups, where R30 carries the definition set forth for Formula I, or more preferably R30 carries the definition set forth for Formula IA.
  • Still other preferred W groups are 4-pyrimidinyl, 5-halo-2-pyrimidinyl, 3,6-dihalopyrimidin-2-yl, and 2,6-, 4,6-, and 5,6-dihalopyridin-2-yl. Other preferred W groups are phenyl substituted with one or two independently selected C1-C2 alkyl, C1-C2 alkoxy, amino, halogen, trifluoromethyl, or cyano groups. Still other preferred W groups are 2-thiazolyl groups carrying one or two independently selected C1-C2 alkyl, amino, (C1-C3) alkyl, hydroxy, (C1-C3)alkyl, or trifluoromethyl groups.
  • Another aspect of the invention includes compounds of Formula III or Formula IV wherein
  • R is independently selected at each occurrence from the group consisting of hydrogen, halogen, and (C1-C2)alkyl;
  • R1, R4, and one of R2 and R3 are independently selected from hydrogen, halogen, trifluoromethyl, C1-C2 alkyl, and cyano;
  • the other of R2 and R3 carries the definition set forth for Formula I, or more preferably for Formula IA; and
  • R5 represents (C1-C6)alkyl, and preferably C2-C4 alkyl. Preferably R1 and R4 are hydrogen;
  • Preferred R groups are independently selected from hydrogen and C1-C3 alkyl, more preferably hydrogen and methyl, and most preferably are hydrogen.
  • More preferred R5 groups are ethyl and n-propyl.
  • In this aspect,
  • Q (in Formula III) is CH2, and R6 and R7 in Formula IV are hydrogen; and
  • W, is phenyl, pyridyl, or thiazolyl, each which is optionally substituted by one or more substituents independently chosen from halogen, cyano, hydroxy, oxo, C1-C2haloalkyl, C1-C2alkyl, and C1-C2 alkoxy, or more preferably
  • W is 2-thiazolyl, 2-pyrimidinyl, 3-fluorophenyl, or 6-fluoro-2-pyridinyl. Such compounds will be referred to as compounds of Formula III-A and Formula IV-A.
  • A particular aspect of the invention provides compounds of Formula III-A and Formula IV-A, wherein:
  • one of R2 and R3 is chosen from hydrogen, halogen, hydroxy, nitro, cyano, amino, (C1-C6)alkyl, (C1-C6)alkoxy, (C3-C8)cycloalkyl, (C3-C8)cycloalkyl(C1-C6)alkyl, halo(C1-C6)alkyl, halo(C1-C6)alkoxy, mono or di(C1-C6)alkylamino, amino(C1-C6)alkyl, and mono- and di(C1-C6)alkylamino(C1-C6)alkyl;
  • the other of R2 and R3 is chosen from
    • i) hydrogen, halogen, hydroxy, nitro, cyano, amino, halo(C1-C6)alkyl, and halo(C1-C6)alkoxy, and
    • ii) C1-C6alkyl, C1-C6alkoxy, C3-C8cycloalkyl, C2-C6alkenyl, C2-C6alkynyl, (C3-C8cycloalkyl)C1-C4alkyl, —NH(R10), —N(R10)(R11) (R10)NH(C1-C6)alkyl, (R10)(R11) N(C1-C6)alkyl, (heterocycloalkyl)alkyl, and heterocycloalkyl, each of which is optionally substituted with 1, 2, 3, or 4 of R20; and
    • R20 carries the definition set forth for Formula I, or more preferably R20 carries the definition set forth with respect to Formula IA.
  • In this aspect, preferred compounds of Formula III-A and IV-A include those where one of R2 and R3 is hydrogen, halogen, hydroxy, nitro, cyano, amino, C1-C3 alkyl, C1-C2 alkoxy, cyclopropyl, cyclopropylmethyl, trifluoromethyl, or mono- or di(C1-C2)alkylamino, and the other is hydrogen, halogen, or C1-C3 alkyl, preferably hydrogen or methyl. More preferred compounds of Formula IV-A include those where R2 is hydrogen, halogen, more preferably fluoro or chloro, cyano, amino, C1-C2 alkyl or C1-C2 alkoxy and R3 is hydrogen or methyl. Other more preferred compounds of Formula IV-A include those where R2 is hydrogen, methyl, or ethyl, and R3 is hydrogen, halogen, preferably fluoro or chloro, cyano, amino, or C1-C3 alkoxy.
  • Another aspect of the invention provides compounds of Formula III-A and Formula IV-A, wherein:
  • one of R2 and R3 is chosen from hydrogen, halogen, hydroxy, nitro, cyano, amino, (C1-C6)alkyl, (C1-C6)alkoxy, (C3-C8)cycloalkyl, (C3-C8)cycloalkyl(C1-C6)alkyl, halo(C1-C6)alkyl, halo(C1-C6)alkoxy, mono or di(C1-C6)alkylamino, amino(C1-C6)alkyl, and mono- and di(C1-C6)alkylamino(C1-C6)alkyl;
  • the other of R2 and R3 is chosen from
  • Figure US20100267698A1-20101021-C00011
  • where J is N, CH, or C— (C1-C6)alkyl and
    • RB and RC are independently selected from the group consisting of hydrogen, (C1-C6)alkyl, (C2-C6)alkenyl, (C2-C6)alkynyl, C3-C8)cycloalkyl, and (C3-C8cycloalkyl) (C1-C4)alkyl; or
      • RB and RC and the atom to which they are attached form a 4- to 10-membered monocyclic or bicyclic ring, which may contain
      • a) one or more double bonds,
      • b) one or more of oxo, O, S, SO, SO2, and N—RD wherein RD is hydrogen or (C1-C6)alkyl;
      • c) one or more of R20; and
  • R20 carries the definition set forth with respect to Formula I, or more preferably R20 carries the definition set forth for R20 Formula IA.
  • The invention also provides compounds of Formula III-A and Formula IV-A, wherein:
  • one of R2 and R3 is chosen from hydrogen, halogen, hydroxy, nitro, cyano, amino, (C1-C6)alkyl, (C1-C6)alkoxy, (C3-C8)cycloalkyl, (C3-C8)cycloalkyl(C1-C6)alkyl, halo(C1-C6)alkyl, halo(C1-C6)alkoxy, mono or di(C1-C6)alkylamino, amino(C1-C6)alkyl, and mono- and di(C1-C6)alkylamino(C1-C6)alkyl;
  • the other of R2 and R3 is a group of the formula:
  • Figure US20100267698A1-20101021-C00012
  • where G is a bond or C1-C2alkyl;
    RA is a saturated, partially unsaturated, or aromatic carbocycle, consisting of 1 ring or 2 fused, pendant, or spiro rings, each ring containing 0, 1, or 2 heteroatoms independently chosen from N, S, and O, said saturated, partially unsaturated, or aromatic carbocycle is optionally substituted with 1, 2, 3, or 4 of R20; and
  • R20 carries the definition set forth for Ra in Formula I, or more preferably R20 carries the definition set forth for R20 in Formula IA R20.
  • Preferably RA is chosen from phenyl, pyrrolyl, pyrazolyl, thiazolyl, isoxazolyl, triazolyl, tetrazolyl, oxadiazolyl, and oxazolyl each of which is optionally substituted with 1, 2, 3, or 4 of R20.
  • Other compounds of the invention are compounds of Formula III-A and Formula IV-A, wherein:
  • one of R2 and R3 is chosen from hydrogen, halogen, hydroxy, nitro, cyano, amino, (C1-C6)alkyl, (C1-C5)alkoxy, (C3-C8)cycloalkyl, (C3-C8)cycloalkyl(C1-C6)alkyl, halo(C1-C6)alkyl, halo(C1-C6)alkoxy, mono or di(C1-C6)alkylamino, amino(C1-C6)alkyl, and mono- and di(C1-C6)alkylamino(C1-C6)alkyl; and
  • the other of R2 and R3 is —HC═N—OH or —HC═N(C1-C6alkoxy).
  • Another aspect of the invention is directed to compounds of Formula I, Formula IA and Formula II wherein
  • one and only one of Z1 is CR1, Z4 is CR4, either Z2 or Z3 is nitrogen; and
  • i) W represents a 5-membered heteroaryl group, and the 5-membered heteroaryl group is optionally substituted with up to 4 groups independently selected from R30, —CO2H, —C(═O)ORE, —C(═O)NHRE, —C(═O)NRERF, —C(O)RE, and —S(O)nRE, —ORE; or
  • ii) W represents a 6-membered aryl or heteroaryl group, wherein the 6-membered aryl or heteroaryl group is optionally substituted with up to 4 groups independently selected from R30, —CO2H, —C(═O)ORE, —C(═O)NHRE, —C(═O)NRERF, —C(O)RE, and —S(O)nRE, —ORE; where
  • R30 and RE carry the definitions set forth for those groups in Formula I, or preferably for Formula IA, and m is 0, 1, or 2.
  • Thus, the invention includes compounds represented by Formula V and Formula VI
  • Figure US20100267698A1-20101021-C00013
  • wherein R, R1, R2, R3, R4, R5, and Q carry the definitions set forth for Formula I, or more preferably for Formula IA, and W is a 5-membered heteroaryl group as described above.
  • The invention further includes compounds of Formula V and Formula VI wherein
  • R is independently selected at each occurrence from the group consisting of
    • i) hydrogen, halogen, (C1-C6)alkyl, (C3-C8)cycloalkyl, (C3-C8)cycloalkyl(C1-C6)alkyl, (C1-C6)alkoxy, halo(C3-C8)alkyl, halo(C1-C6)alkoxy, and
    • ii) phenyl and pyridyl each of which is optionally substituted with up to 3 substituents independently chosen from halogen, hydroxy, C1-4alkyl, and —O(C1-4alkyl);
  • R1, R2, R3, and R4, are independently selected from hydrogen, halogen, hydroxy, nitro, cyano, amino, (C1-C6)alkyl, (C1-C6)alkoxy, (C3-C8)cycloalkyl, (C3-C8)cycloalkyl(C1-C6)alkyl, (C2-C6)alkenyl, (C2-C6)alkynyl, heterocycloalkyl, halo(C1-C6)alkyl, halo(C1-C6)alkoxy, mono or di(C1-C6)alkylamino, amino(C1-C6)alkyl, and mono- and di(C1-C6)alkylamino(C1-C6)alkyl;
  • R5 represents hydrogen, (C1-C6)alkyl, (C3-C8)cycloalkyl, (C3-C8)cycloalkyl(C1-C6)alkyl, phenyl, benzyl, thiophenyl, thiazoyl, pyridyl, imidazolyl, pyrazolyl, or pyrimidinyl;
  • R6 and R7 independently represent hydrogen, fluorine, or C1-C6 alkyl;
  • W represents either a 5-membered heteroaryl group chosen from thienyl, thiazolyl, imidazolyl, oxazolyl, triazolyl, tetrazolyl, pyrazolyl, or isoxazolyl each of which is optionally substituted with up to 4 R30 groups; or
  • W represents a 6-membered aryl or heteroaryl group chosen from phenyl, pyrimidinyl, pyridyl, pyridizinyl, or pyrazinyl, each of which is optionally substituted with up to 4 R30 groups; and
  • R30 is as defined for Formula I, or preferably as defined for Formula IA.
  • Preferred compounds of Formula V an VI include those where R2 and R3 independently represent hydrogen, halogen, preferably fluoro or chloro, C1-C3 alkyl, cyclopropyl, cyclopropylmethyl, C1-C3 alkoxy, trifluoromethyl, nitro, cyano, amino, or mono- or di(C1-C3)alkylamino. Other preferred R2 and R3 groups are mono- or di(C1-C3)alkylamino(C2-C3)alkoxy, morpholinyl(C2-C3)alkoxy, piperidin-1-yl(C2-C3)alkoxy, and piperazin-1-yl(C2-C3)alkoxy.
  • In another aspect, the invention is directed to compounds of Formula V and Formula VI wherein:
  • R is independently selected at each occurrence from the group consisting of hydrogen, halogen, and (C1-C2)alkyl; and
  • R1 and R4 are independently selected from hydrogen, halogen, hydroxy, nitro, cyano, amino, (C1-C8)alkyl, (C1-C6)alkoxy, (C3-C8)cycloalkyl, (C3-C8)cycloalkyl(C1-C6)alkyl, halo(C1-C6)alkyl, halo(C1-C6)alkoxy, mono or di(C1-C6)alkylamino, amino(C1-C6)alkyl, and mono- and di(C1-C6)alkylamino(C1-C6)alkyl.
  • In this aspect of the invention,
  • R2 (Formula V) and R3 (Formula VI) carry the definitions set forth with respect to Formula I, or more preferably for Formula IA;
  • R5 represents (C1-C10)alkyl, preferably ethyl or n-propyl;
  • R6 and R7 are hydrogen;
  • W represents a 5-membered heteroaryl group chosen from furanyl, thienyl, thiazoyl, imidazolyl, oxazolyl, triazolyl, tetrazolyl, pyrazolyl, or isoxazolyl, each of which is optionally substituted with up to 4 R30 groups, or
  • W represents a 6-membered aryl or heteroaryl group chosen from phenyl, pyrimidinyl, pyridyl, pyridizinyl, or pyrazinyl each of which is optionally substituted with up to 4 R30 groups; and
  • R30 is as defined for Formula I, or more preferably for Formula IA.
  • Compounds of this aspect of the invention will be referred to as compounds of Formula V-A and Formula VI-A.
  • Preferred R groups are hydrogen and C1-C3 alkyl, more preferably hydrogen and methyl, and most preferably hydrogen.
  • Preferred R1 and R4 groups for this aspect of the invention include hydrogen, halogen, trifluoromethyl, C1-C2 alkyl, and cyano. Preferably R1 and R4 are hydrogen. More preferably R, R1, and R4 are all hydrogen.
  • Preferred compounds of this aspect of the invention in which W is a 5-membered heteroaryl group include those wherein W is thiazolyl which is optionally substituted by one or more substituents independently chosen from halogen, cyano, hydroxy, oxo, C1-C2haloalkyl, C1-C2alkyl, and C1-C2 alkoxy. Other preferred compounds are those wherein W is 2-thiazolyl.
  • Preferred compounds of this aspect in which W is a 6-membered heteroaryl group include those wherein W is phenyl or pyridyl, each of which is optionally substituted by one or more substituents independently chosen from halogen, cyano, hydroxy, oxo, C1-C2haloalkyl, C1-C2alkyl, and C1-C2 alkoxy. Also preferred are compounds wherein W is 2-pyrimidinyl, 3-fluorophenyl, or 6-fluoro-2-pyridinyl.
  • Still other preferred W groups are 4-pyrimidinyl, 5-halo-2-pyrimidinyl, 3,6-dihalopyrimidin-2-yl, and 2,6-, 4,6-, and 5,6-dihalopyridin-2-yl. Other preferred W groups are phenyl substituted with one or two independently selected C1-C2 alkyl, C1-C2 alkoxy, amino, halogen, trifluoromethyl, or cyano groups. Still other preferred W groups are 2-thiazolyl groups carrying one or two independently selected C1-C2 alkyl, amino, (C1-C3) alkyl, hydroxy, (C1-C3)alkyl, or trifluoromethyl groups.
  • Other aspects of the invention include compounds of Formula V-A and Formula VI-A wherein
  • R2 (for Formula V-A) or R3 (for Formula VI-A) is chosen from
  • i) hydrogen, halogen, hydroxy, nitro, cyano, amino, halo(C1-C6)alkyl, and halo(C1-C6)alkoxy,
  • ii) C1-C6alkyl, C1-C6alkoxy, C3-C8cycloalkyl, C2-C6alkenyl, C1-C6alkynyl, (C3-C8cycloalkyl)C1-C4alkyl, —NH(R10), —N(R10)(R11), (R10)NH(C1-C6)alkyl, (R10)(R11)N(C1-C6)alkyl, (heterocycloalkyl) C1-C4alkyl, and heterocycloalkyl, each of which is optionally substituted with 1, 2, 3, or 4 of R20, where R20 carries the definition set forth for compounds of Formula I, or preferably for compounds of Formula IA.
  • Another aspect of the invention is directed to compounds of Formula V-A and Formula VI-A wherein R2 (for Formula V-A) or R3 (for Formula VI-A) is chosen from hydrogen, halogen, hydroxy, nitro, cyano, amino, halo(C1-C6)alkyl, and halo(C1-C6)alkoxy.
  • Further aspects of the invention include compounds of Formula V-A and Formula VI-A wherein R2 (for Formula V-A) or R3 (for Formula VI-A) is a group of the formula
  • Figure US20100267698A1-20101021-C00014
  • where J is N, CH, or C—(C1-C10)alkyl and
  • RB and RC are independently selected from the group consisting of hydrogen, (C1-C6)alkyl, (C2-C6)alkenyl, (C2-C6)alkynyl, C3-C8)cycloalkyl, and (C3-C8cycloalkyl) (C1-C4)alkyl; or
  • RB and RC and the atom to which they are attached form a 4- to 10-membered monocyclic or bicyclic ring, which may contain a) one or more double bonds, b) one or more of oxo, O, S, SO, SO2, and N—RD wherein RD is hydrogen or (C1-C6)alkyl; and/or c) one or more substituents R20, where R20 carries the definition set forth for compounds of Formula I, or more preferably that set forth for compounds of Formula IA.
  • Also provided by the invention are compounds of Formula V-A and Formula VI-A wherein R2 (for Formula V-A) or R3 (for Formula VI-A) is a group of the formula
  • Figure US20100267698A1-20101021-C00015
  • where G is a bond or C1-C2alkyl; and RA is a saturated, partially unsaturated, or aromatic carbocycle consisting of 1 ring or 2 fused, pendant, or spiro rings, each ring containing 0, 1, or 2 heteroatoms independently selected from N, S, and O, where the saturated, partially unsaturated, or aromatic carbocycle is optionally substituted with 1, 2, 3, or 4 of R20. Preferred compounds of this class are those where RA is chosen from phenyl, pyrrolyl, pyrazolyl, thiazolyl, isoxazolyl, triazolyl, tetrazolyl, oxadiazolyl, and oxazolyl each of which is optionally substituted with 1, 2, 3, or 4 of R20. R20 carries the definition set forth for compounds of Formula I, or more preferably that set forth for compounds of Formula IA.
  • The invention also includes compounds of Formula V-A and Formula VI-A wherein R2 (for Formula V-A) or R3 (for Formula VI-A) is —HC═N—OH or —HC═N(C1-C6alkoxy).
  • Other benzimidazole and pyridylimidazole compounds of the invention are represented by Formula X-Formula XVIII, below.
  • Figure US20100267698A1-20101021-C00016
    Figure US20100267698A1-20101021-C00017
  • The variables Z1, Z2, Z3, Z4, R, Q, and W, which appear in Formulae X-XVIII carry the definition set forth for Formula I, or more preferably for Formula IA.
  • Compounds of Formulae X-XVIII wherein Z1 is CR1, Z2 is CR2, Z3 is CR3, and Z4 is OR4 are preferred. Compounds of Formulae X-XVIII wherein one and only one Z1, Z2, Z3, Z4 is nitrogen are also preferred. Compounds of Formulae X-XVIII wherein one and only one Z1, Z2, Z3, Z4 is nitrogen, and either Z2 or Z3 is nitrogen are particularly preferred.
  • Particularly embodied in the invention are compounds of Formulae X-XVIII wherein Q (when present) is C(R6)(R7). Preferably R6 and R7 are hydrogen.
  • Other embodiments of the invention are directed to compounds of Formulae X-XVIII wherein w represents a 5-membered heteroaryl group, and the 5-membered heteroaryl group is optionally substituted with up to 4 groups independently selected from R30, —CO2H, —C(═O)ORE, —C(═O)NHRE, —C(═O)NRERF, —C(O)RE, and —S(O)nRE, —ORE, where R30 and RE are as defined above and m is 0, 1, or 2. Preferred compounds of this class are compounds wherein wherein Z1 is CR1, Z2 is CR2, Z3 is CR3, and Z4 is CR4 or wherein one and only one Z1, Z2, Z3, Z4 is nitrogen are also preferred; compounds of this class wherein one and only one Z1, Z2, Z3, Z4 is nitrogen, and either Z2 or Z3 is nitrogen are particularly preferred.
  • This invention provides benzimidazole and pyridylimidazole derivatives, preferred examples of which bind with high affinity to the benzodiazepine site of GABAA receptors, including human GABAA receptors. The affinity of compounds of Formula I for the benzodiazepine site may be determined using a GABAA receptor binding assay, such as the assay presented in Example 53. Preferred compounds of Formula I that bind with high affinity to the benzodiazepine site of the GABAA receptor exhibit Ki values of less than 1 μM in that assay, Very high affinity compounds of the invention exhibit Ki values of less than 100 nM or more preferably less than 10 nM in the assay presented in Example 53. Without wishing to be bound to any particular theory, it is believed that the interaction of the compounds of Formula I with the benzodiazepine site results in the pharmaceutical utility of these compounds.
  • Benzimidazole and pyridylimidazole derivatives that bind with high selectivity to the benzodiazepine site of GABAA receptors, including human GABAA receptors, are also included in this invention. Preferred compounds of Formula I which exhibit high selectivity (or high specificity) exhibit affinity for the benzodiazepine site of the GABA receptor that is at least 10-fold greater, and preferably 100-fold greater, than the affinity exhibited at any other membrane-bound receptor which is a known drug target. More preferred compounds of Formula I do not exhibit a binding affinity at any other membrane-bound receptor which is a known drug target that is less than 1 micromolar. Membrane-bound receptors that are known drug targets include, but are not limited to dopamine receptors, CRF receptors, bradykinin receptors, NPY receptors, beta-adrenergic receptors, capsaicin receptors, galanin receptors, MCH receptors, melanocortin receptors, and neurokinin receptors. Binding affinities for membrane-bound receptors which are known drug targets may be determined via radioligand binding assays which are generally well known in the art.
  • The invention further comprises methods of treating patients in need of such treatment with an amount of a compound of the invention sufficient to alter the symptoms of a CNS disorder. Compounds of the inventions that act as agonists at α2β3γ2 and α3β3γ2 receptor subtypes are useful in treating anxiety disorders such as panic disorder, obsessive compulsive disorder and generalized anxiety disorder; stress disorders including post-traumatic stress, and acute stress disorders. Compounds of the inventions that act as agonists at α2β3γ2 and α3β3γ2 receptor subtypes are also useful in treating depressive or bipolar disorders and in treating sleep disorders. Compounds of the invention that act as inverse agonists at the α5β3γ2 receptor subtype or α1β2γ2 and α5β3γ2 receptor subtypes are useful in treating cognitive disorders including those resulting from Down Syndrome, neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease, and stroke related dementia. Compounds of the invention that act as inverse agonists at the α5β3γ2 are particularly useful in treating cognitive disorders through the enhancement of memory, and particularly short-term memory, in memory-impaired patients. Compounds of the invention that act as agonists at the α1β2γ2 receptor subtype are useful in treating convulsive disorders such as epilepsy. Compounds that act as antagonists at the benzodiazepine site are useful in reversing the effect of benzodiazepine overdose and in treating drug and alcohol addiction.
  • The diseases and/or disorders that can also be treated using compounds and compositions according to the invention include:
  • Depression, e.g. depression, atypical depression, bipolar disorder, depressed phase of bipolar disorder.
    Anxiety, e.g. general anxiety disorder (GAD), agoraphobia, panic disorder +/− agoraphobia, social phobia, specific phobia, Post traumatic stress disorder, obsessive compulsive disorder (OCD), dysthymia, adjustment disorders with disturbance of mood and anxiety, separation anxiety disorder, anticipatory anxiety acute stress disorder, adjustment disorders, cyclothymia.
    Sleep disorders, e.g. sleep disorders including primary insomnia, circadian rhythm sleep disorder, dyssomnia NOS, parasomnias, including nightmare disorder, sleep terror disorder, sleep disorders secondary to depression and/or anxiety or other mental disorders, substance induced sleep disorder.
    Cognition Impairment, e.g. cognition impairment, memory impairment, short-term memory impairment, Alzheimer's disease, Parkinson's disease, mild cognitive impairment (MC1), age-related cognitive decline (ARCD), stroke, traumatic brain injury, AIDS associated dementia, and dementia associated with depression, anxiety or psychosis.
    Attention Deficit Disorder, e.g. attention deficit disorder (ADD), and attention deficit and hyperactivity disorder (ADHD).
    Speech disorders, e.g. stuttering, including motor tic, clonic stuttering, dysfluency, speech blockage, dysarthria, Tourete syndrome or logospasm.
    Psychosis e.g. schizophrenia, hallucinatory disorders
  • The invention also provides pharmaceutical compositions comprising one or more compounds of the invention together with a pharmaceutically acceptable carrier or excipient, for treating disorders responsive to GABAA receptor modulation, e.g., treatment of anxiety, depression, sleep disorders or cognitive impairment by GABAA receptor modulation. Pharmaceutical compositions include packaged pharmaceutical compositions comprising a container holding a therapeutically effective amount of at least one GABAA receptor modulator as described supra and instructions (e.g., labeling) indicating the contained GABAA receptor ligand is to be used for treating a disorder responsive to GABAA receptor modulation in the patient.
  • In a separate aspect, the invention provides a method of potentiating the actions of other CNS active compounds, which comprises administering an effective amount of a compound of the invention in combination with another CNS active compound. Such CNS active compounds include, but are not limited to the following: for anxiety, serotonin receptor (e.g. 5-HT1A) agonists and antagonists; for anxiety and depression, neurokinin receptor antagonists or corticotropin releasing factor receptor (CRF1) antagonists; for sleep disorders, melatonin receptor agonists; and for neurodegenerative disorders, such as Alzheimer's dementia, nicotinic agonists, muscarinic agents, acetylcholinesterase inhibitors and dopamine receptor agonists. Particularly the invention provides a method of potentiating the antidepressant activity of selective serotonin reuptake inhibitors (SSRIs) by administering an effective amount of a GABA agonist compound of the invention in combination with an SSRI.
  • Combination administration can be carried out in a fashion analogous to that disclosed in Da-Rocha, et al., J. Psychopharmacology (1997) 11(3) 211-218; Smith, et al., Am. J. Psychiatry (1998) 155(10) 1339-45; or Le, et al., Alcohol and Alcoholism (1996) 31 Suppl. 127-132. Also see, the discussion of the use of the GABAA receptor ligand 3-(5-methylisoxazol-3-yl)-6-(1-methyl-1,2,3-triazol-4-yl)methyloxy-1,2,4-triazolo[3,4-a]phthalzine in combination with nicotinic agonists, muscarinic agonists, and acetylcholinesterase inhibitors, in PCT International publications Nos. WO 99/47142, WO 99/47171, and WO 99/47131, respectively. Also see in this regard PCT International publication No. WO 99/37303 for its discussion of the use of a class of GABAA receptor ligands, 1,2,4-triazolo[4,3-b]pyridazines, in combination with SSRIs.
  • The present invention also pertains to methods of inhibiting the binding of benzodiazepine compounds, such as Ro15-1788, or GABA to the GABAA receptors which methods involve contacting a solution containing compound of the invention with cells expressing GABAA receptors, wherein the compound is present at a concentration sufficient to inhibit benzodiazepine binding or GABA binding to GABAA receptors in vitro. This method includes inhibiting the binding of benzodiazepine compounds to GABAA receptors in vivo, e.g., in a patient given an amount of a compound of Formula I that would be sufficient to inhibit the binding of benzodiazepine compounds or GABA to GABAA receptors in vitro. In one embodiment, such methods are useful in treating benzodiazepine drug overdose. The amount of a compound that would be sufficient to inhibit the binding of a benzodiazepine compound to the GABAA receptor may be readily determined via a GABAA receptor binding assay, such as the assay described in Example 53. The GABAA receptors used to determine in vitro binding may be obtained from a variety of sources, for example from preparations of rat cortex or from cells expressing cloned human GABAA receptors.
  • The invention also provides methods for altering the signal-transducing activity, particularly the chloride ion conductance of GABAA receptors, said method comprising exposing cells expressing such receptors to an effective amount of a compound of the invention. This method includes altering the signal-transducing activity of GABAA receptors in vivo, e.g., in a patient given an amount of a compound of Formula I that would be sufficient to alter the signal-transducing activity of GABAA receptors in vitro. The amount of a compound that would be sufficient to alter the signal-transducing activity of GABAA receptors may be determined via a GABAA receptor signal transduction assay, such as the assay described in Example 54. The cells expressing the GABA receptors in vivo may be, but are not limited to, neuronal cells or brain cells. Such cells may be contacted with compounds of the invention through contact with a body fluid containing the compound, for example through contact with cerebrospinal fluid. Alteration of the signal-transducing activity of GABAA receptors in vitro may be determined from a detectable change in the electrophysiology of cells expressing GABAA receptors, when such cells are contacted with an compound of the invention in the presence of GABA. For example, a change in the electrophysiology of cells expressing GABAA receptors may be detected using a voltage-clamp assay performed on oocytes injected with GABAA receptor mRNA. Such an assay is shown in Example 54.
  • Intracellular recording or patch-clamp recording may be used to quantitate changes in electrophysiology of cells. A reproducible change in behavior of an animal given a compound of the invention may also be used to indicate that changes in the electrophysiology of the animal's cells expressing GABAA receptors has occurred.
  • The GABAA receptor ligands provided by this invention and labeled derivatives thereof are also useful as standards and reagents in determining the ability of a potential pharmaceutical to bind to the GABAA receptor. Radiolabeled derivatives the GABAA receptor ligands provided by this invention are also useful as radiotracers for positron emission tomography (PET) imaging or for single photon emission computerized tomography (SPECT).
  • More particularly compounds of the invention may be used for demonstrating the presence of GABAA receptors in cell or tissue samples. This may be done by preparing a plurality of matched cell or tissue samples, at least one of which is prepared as an experimental sample and at least one of which is prepared as a control sample. The experimental sample is prepared by contacting (under conditions that permit binding of RO15-1788 to GABAA receptors within cell and tissue samples) at least one of the matched cell or tissue samples that has not previously been contacted with any compound or salt of the invention with an experimental solution comprising the detectably-labeled preparation of the selected compound or salt at the first measured molar concentration. The control sample is prepared in the same manner as the experimental sample and also contains an unlabelled preparation of the same compound or salt of the invention at a greater molar concentration.
  • The experimental and control samples are then washed to remove unbound detectably-labeled compound. The amount of remaining bound detectably-labeled compound is then measured and the amount of detectably-labeled compound in the experimental and control samples is compared. A comparison that indicates the detection of a greater amount of detectable label in the at least one washed experimental sample than is detected in any of control samples demonstrates the presence of GABAA receptors in that experimental sample.
  • The detectably-labeled compound used in this procedure may be labeled with a radioactive label or a directly or indirectly luminescent label. When tissue sections are used in this procedure and the detectably-labeled compound is radiolabeled, the bound, labeled compound may be detected autoradiographically to generate an autoradiogram. The amount of detectable label in an experimental or control sample may be measured by viewing the autoradiograms and comparing the exposure density of the autoradiograms.
  • The invention provides a method for preparing a compound of Formula A
  • Figure US20100267698A1-20101021-C00018
  • which comprises reacting a compound of Formula B
  • Figure US20100267698A1-20101021-C00019
  • with a compound of Formula C
  • Figure US20100267698A1-20101021-C00020
  • In Formula A and C, above Z1, Z2, Z3, Z4, and R5 carry the definitions forth for Formula I, or more preferably Z1, Z2, Z3, Z4, and R5 carry the definitions forth for Formula Ia.
  • R6 and R7 independently represent hydrogen, fluorine, or alkyl.
  • R in Formula B, above, is independently chosen at each occurrence from hydrogen, halogen, amino, C1-C6alkyl, (C2-C6)alkenyl, (C2-C6)alkynyl, C1-C6alkoxy, (C3-C8)cycloalkyl, (C3-C8cycloalkyl) (C1-C4)alkyl, halo(C1-C6)alkyl, haloalkoxy, carboxamido, and 3- to 7-membered carbocyclic or heterocyclic groups which are saturated, unsaturated, or aromatic, which may be further substituted with one or more substituents independently selected from halogen, oxo, hydroxy, C1-4alkyl, and —O(C1-4alkyl).
  • W in Formula B represents aryl or heteroaryl, wherein the aryl or heteroaryl group is optionally substituted with up to 4 groups independently selected from R30, —CO2H, C(═O)ORE, —C(═O)NHRE, —C(═O)NRERF, —C(O)RE, and —S(O)mRE, —ORE, where R30 and RE are as for Formula I or preferably as defined for Formula Ia and m is 0, 1, or 2. This process will be referred to as Process 1.
  • In particular embodiments the invention includes a process of preparing a compound of Formula A as described above wherein: Z1 is CR1, Z2 is CR2, Z3 is CR3, and Z4 is CR4.
  • R is independently selected at each occurrence from the group consisting of hydrogen, halogen, and (C1-C2)alkyl;
  • R1, R4, and one of R2 and R3 are independently selected from hydrogen, halogen, hydroxy, nitro, cyano, amino, (C1-C5)alkyl, (C1-C6)alkoxy, (C3-C8)cycloalkyl, (C3-C8)cycloalkyl(C1-C6)alkyl, halo(C1-C6)alkyl, halo(C1-C6)alkoxy, mono or di(C1-C6)alkylamino, amino(C1-C6)alkyl, and mono- and di(C3-C6)alkylamino(C1-C6)alkyl.
  • The other of R2 and R3 carries the definition set forth for Formula I, or preferably that set forth for Formula Ia, or in certain preferred embodiments this group is chosen from
    • i) hydrogen, halogen, hydroxy, nitro, cyano, amino, halo(C1-C6)alkyl, and halo(C1-C6)alkoxy,
    • ii) C1-C6alkyl, C1-C6alkoxy, C3-C8cycloalkyl, C2-C6alkenyl, C2-C6alkynyl, (C3-C8cycloalkyl) C1-C4alkyl, —NH(R10), —N(R10)(R11), (R10)NH(C1-C6)alkyl, (R10)(R11)N(C1-C6)alkyl, (heterocycloalkyl)C1-C4alkyl, and heterocycloalkyl, each of which is optionally substituted with 1, 2, 3, or 4 of R20.
  • In certain preferred embodiments R, R1, and R4 are all hydrogen.
  • R5 represents (C1-C6)alkyl. Preferred definitions of R5 include ethyl and n-propyl.
  • R6 and R7 are hydrogen.
  • W represents phenyl, furanyl, thienyl, thiazolyl, imidazolyl, oxazolyl, triazolyl, tetrazolyl, pyrazolyl, isoxazolyl, pyrimidinyl, benzimidazolyl, quinolinyl, isoquinolinyl each of which is optionally substituted with up to 4 R30 groups, where R30 is as defined in the above process. Preferred W groups include, 2-thiazolyl, 2-pyrimidinyl, 3-fluorophenyl, or 6-fluoro-2-pyridinyl.
  • In other preferred embodiments the invention is directed to a process, as described as for Process 1, wherein Z1 is CR1; one and only one of Z2 or Z2 is nitrogen; Z4 is CR4.
  • R1 and R4 may carry the definition set forth in Process 1. Preferred definitions of R1 and R4 include hydrogen, halogen, trifluoromethyl, C1-C2 alkyl, and cyano.
  • In certain preferred embodiments R, R1, and R4 are all hydrogen.
  • R2 or R3 (for whichever one of Z2 or Z3 is CR2 or CR3) is chosen from
  • i) hydrogen, halogen, hydroxy, nitro, cyano, amino, halo(C1-C6)alkyl, and halo(C1-C6)alkoxy,
  • ii) C1-C6alkyl, C1-C6alkoxy, C3-C8cycloalkyl, C2-C6alkenyl, C2-C6alkynyl, (C3-C8cycloalkyl) C1-C4alkyl, —NH(R10), —N(R10)(R11) (R10)NH(C1-C6)alkyl, (R10)(R11)N(C3-C6)alkyl, (heterocycloalkyl)C1-C4alkyl, and heterocycloalkyl, each of which is optionally substituted with 1, 2, 3, or 4 of R20.
  • R5 represents (C1-C6)alkyl. Preferred definitions of R5 include ethyl and n-propyl.
  • R6 and R7 are hydrogen.
  • W represents a 5-membered heteroaryl group, the 5-membered heteroaryl group is optionally substituted with up to 4 groups independently selected from R30, —CO2H, —C(═O)ORE, —C(═O)NHRE, —C(═O)NRERF, —C(O)RE, and —S(O)mRE, —ORE, where R30 and RE are as defined above and m is 0, 1, or 2; or
  • W represents a 6-membered aryl or heteroaryl group, wherein the 6-membered aryl or heteroaryl group is optionally substituted with up to 4 groups independently selected from R30, —CO2H, —C(═O)ORE, —C(═O)NHRE, —C(═O)NRERF, —C(O)RE, and —S(O)mRE, —ORE, where R30 and RE are as defined above and m is 0, 1, or 2.
  • When W represents a 5-membered heteroaryl group W is preferably thiazolyl, thienyl, imidazolyl, oxazolyl, triazolyl, tetrazolyl, pyrazolyl, or isoxazolyl, each of which is optionally substituted by one or more substituents independently chosen from halogen, cyano, hydroxy, oxo, C1-C2haloalkyl, C1-C2alkyl, and C1-C2 alkoxy. Unsubstituted 2-thiazolyl is a particularly preferred W group.
  • When W represents a 6-membered aryl or heteroaryl group, W is preferably phenyl, pyrimidinyl, pyridyl, pyrazinyl, or pyridizinyl, each of which is optionally substituted by one or more substituents independently chosen from halogen, cyano, hydroxy, oxo, C1-C2haloalkyl, C1-C2alkyl, and C1-C2 alkoxy. Particularly preferred W groups include 2-pyrimidinyl, 3-fluorophenyl, or 6-fluoro-2-pyridinyl.
  • In this process the reactants B and C are generally combined in a polar aprotic solvent, such as THF, DMF, or 1,4-dioxane, at temperatures ranging from 0-100 degrees C. A reducing agent such as NaH or other base, for example sodium hydroxide, potassium butoxide, potassium carbonate, or cesium carbonate, is then added, and the reaction is allowed to proceed. Choice of solvent, reaction temperature, and reducing agent will depend on the identity of the reactants B and C, but will be readily determined by a worker of ordinary skill in the art of chemical synthesis. Scheme I, step 4, provides further illustration of this process.
    • Chemical Description and Terminology
  • Formula I includes, but is not limited to the subformulae exemplified as Formula Ia, Formulae II-VI and Formulae X-XVIII and their pharmaceutically acceptable acid and base addition salts.
  • The phrase “pharmaceutically acceptable” is employed herein to refer to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings or animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio. As used herein, “pharmaceutically acceptable salts” refer to derivatives of the disclosed compounds wherein the parent compound is modified by making acid or base salts thereof. Those skilled in the art will recognize a wide variety of non-toxic pharmaceutically acceptable addition salts.
  • Examples of pharmaceutically acceptable salts include, but are not limited to, mineral or organic acid salts of basic residues such as amines; alkali or organic salts of acidic residues such as carboxylic acids; and the like. The pharmaceutically acceptable salts include the conventional non-toxic salts or the quaternary ammonium salts of the parent compound formed, for example, from non-toxic inorganic or organic acids. For example, such conventional non-toxic salts include those derived from inorganic acids such as hydrochloric, hydrobromic, hydroiodic, sulfuric, sulfamic, sulfinic, phosphoric, nitric and the like; and the salts prepared from organic acids such as alkanoic such as acetic, HOOC—(CH2)n-ACOOH where n is 0-4, and the like, tartaric, maleic, propionic, succinic, glycolic, stearic, lactic, malic, tartaric, citric, ascorbic, pamoic, malefic, hydroxymaleic, phenylacetic, glutamic, benzoic, salicylic, sulfanilic, 2-acetoxybenzoic, fumaric, toluenesulfonic, methanesulfonic, ethane disulfonic, oxalic, isethionic, HOOC—(CH2)n—COOH where n is 0-4, and the like. The pharmaceutically acceptable salts of the present invention can be synthesized from the parent compound which contains a basic or acidic moiety by conventional chemical methods. Generally, such salts can be prepared by reacting the free acid or base forms of these compounds with a stoichiometric amount of the appropriate base or acid in water or in an organic solvent, or in a mixture of the two; generally, nonaqueous media like ether, ethyl acetate, ethanol, isopropanol, or acetonitrile are preferred. Lists of suitable salts are found in Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, Pa., p. 1418 (1985).
  • The invention includes hydrates of compounds of Formula I.
  • The invention includes all crystalline forms of the compounds of Formula I. Certain crystalline forms may be preferred.
  • The present invention also encompasses the acylated prodrugs of the compounds of Formula I. Those skilled in the art will recognize various synthetic methodologies that may be employed to prepare non-toxic pharmaceutically acceptable addition salts and acylated prodrugs of the compounds encompassed by Formula I. The invention further encompasses all enantiomers and diastereomers of the disclosed compounds. Those of ordinary skill in the art will readily recognize methods by which mixtures of enantiomers and diasteromers may be resolved. The definition of Formula I as used in herein include possible isomers, such as tautomers and rotamers.
  • The compounds herein described may have one or more asymmetric centers or planes. Compounds of the present invention containing an asymmetrically substituted atom may be isolated in optically active or racemic forms. It is well known in the art how to prepare optically active forms, such as by resolution of racemic forms (racemates), by asymmetric synthesis, or by synthesis from optically active starting materials. Resolution of the racemates can be accomplished, for example, by conventional methods such as crystallization in the presence of a resolving agent, or chromatography, using, for example a chiral HPLC column. Many geometric isomers of olefins, C═N double bonds, and the like can also be present in the compounds described herein, and all such stable isomers are contemplated in the present invention. Cis and trans geometric isomers of the compounds of the present invention are described and may be isolated as a mixture of isomers or as separated isomeric forms. All chiral (enantiomeric and diastereomeric), and racemic forms, as well as all geometric isomeric forms of a structure are intended, unless the specific stereochemistry or isomeric form is specifically indicated.
  • When any variable occurs more than one time in any constituent or formula for a compound, its definition at each occurrence is independent of its definition at every other occurrence. Thus, for example, if a group is shown to be substituted with 0 to 3 R*, (where R* indicates any variable group such as R) then said group may optionally be substituted with up to three R* groups and R* at each occurrence is selected independently from the definition of R*. Also, combinations of substituents and/or variables are permissible only if such combinations result in stable compounds.
  • When any group, such as an aryl group, heteroaryl group, carbocyclic group, heterocyclic group, or monocylic or bicyclic ring is said to be “optionally substituted by one or more substituents” that group may contain 0 or from 1 to the maximum number of substituents allowable without exceeding the valency of the atoms of the substituted group. Preferably such groups are substituted with 0 or from 1 to 4 substituents, and more preferably such groups are substituted with 0 or from 1 to 3 substituents. Preferably such groups are not substituted with more that one oxo substituent.
  • A dash “-” that is not between two letters or symbols is used to indicate a point of attachment for a substituent. For example —C(═O)NH2 is attached through the carbon atom.
  • As used herein, “alkyl” is intended to include both branched and straight-chain aliphatic hydrocarbon groups, having the specified number of carbon atoms. Alkyl groups of 2 or more carbon atoms may contain double or triple bonds. Examples of alkyl include, but are not limited to, methyl, ethyl, n-propyl, i-propyl, n-butyl, s-butyl, t-butyl, n-pentyl, and s-pentyl. Preferred alkyl groups are C1-C6 alkyl groups. “C1-C6 alkyl” indicates alkyl groups having from 1 to about 6 carbon atoms.
  • As used herein, “alkoxy” represents an alkyl group as defined above with the indicated number of carbon atoms attached through an oxygen bridge. Examples of alkoxy include, but are not limited to, methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy, 2-butoxy, t-butoxy, n-pentoxy, 2-pentoxy, 3-pentoxy, isopentoxy, neopentoxy, n-hexoxy, 2-hexoxy, 3-hexoxy, and 3-methylpentoxy. “C1-C6 alkoxy” indicates alkoxy groups having from 1 to about 6 carbon atoms.
  • “Alkenyl” is intended to include hydrocarbon chains of either a straight or branched configuration comprising one or more unsaturated carbon-carbon bonds which may occur in any stable point along the chain, such as ethenyl and propenyl. Alkenyl groups typically will have 2 to about 8 carbon atoms, more typically 2 to about 6 carbon atoms.
  • “Alkynyl” is intended to include hydrocarbon chains of either a straight or branched configuration comprising one or more triple carbon-carbon bonds which may occur in any stable point along the chain, such as ethynyl and propynyl. Alkynyl groups typically will have 2 to about 8 carbon atoms, more typically 2 to about 6 carbon atoms.
  • “Aryl” refers to aromatic groups having 1 or more rings, wherein the members of the aromatic ring or rings are carbon. When indicated such groups may be substituted. Preferred aryl groups include optionally substituted phenyl and optionally substituted naphthyl.
  • The term “Cycloalkyl” is intended to include saturated ring groups, having the specified number of carbon atoms, such as cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl. Cycloalkyl groups typically will have 3 to about 8 ring members.
  • In the term “(cycloalkyl)alkyl”, Cycloalkyl and alkyl are as defined above and the point of attachment is on the alkyl group. This term encompasses, but is not limited to, cyclopropylmethyl, cyclohexylmethyl, cyclohexylmethyl.
  • As used herein, “haloalkyl” is intended to include both branched and straight-chain saturated aliphatic hydrocarbon groups having the specified number of carbon atoms, substituted with 1 or more halogen atoms (for example —CvFW where v=1 to 3 and w=1 to (2v+1). Examples of haloalkyl include, but are not limited to, trifluoromethyl, difluoromethyl, trichloromethyl, pentafluoroethyl, and pentachloroethyl.
  • As used herein, “haloalkoxy” indicates a haloalkyl group as defined above with the indicated number of carbon atoms attached through an oxygen bridge. Examples of haloalkoxy groups include, but are not limited to, trifluoromethoxy and trichloromethoxy.
  • As used herein the term “heteroaryl” is intended to mean a stable 5- to 7-membered monocyclic or 7- to 10-membered bicyclic heterocyclic aromatic ring which consists of carbon atoms and from 1 to 4 heteroatoms independently selected from the group consisting of N, O and S. It is preferred that the total number of S and O atoms in the heteroaryl group is not more than 1.
  • Examples of heteroaryl groups include, but are not limited to, pyrimidinyl, pyridyl, quinolinyl, benzothienyl, indolyl, pryidazinyl, pyazinyl, isoindolyl, isoquinolyl, quinazolinyl, quinoxalinyl, phthalazinyl, imidazolyl, isoxazolyl, pyrazolyl, oxazolyl, thienyl, thiazolyl, indolizinyl, indazolyl, benzothiazolyl, benzimidazolyl, benzofuranyl, benzoisoxolyl, dihydro-benzodioxinyl, furanyl, pyrrolyl, oxadiazolyl, thiadiazolyl, triazolyl, tetrazolyl, oxazolopyridinyl, imidazopyridinyl, isothiazolyl, naphthyridinyl, cinnolinyl, carbazolyl, beta-carbolinyl, isochromanyl, chromanonyl, chromanyl, tetrahydroisoquinolinyl, isoindolinyl, isobenzotetrahydrofuranyl, isobenzotetrahydrothienyl, isobenzothienyl, benzoxazolyl, pyridopyridinyl, benzotetrahydrofuranyl, benzotetrahydrothienyl, purinyl, benzodioxolyl, triazinyl, phenoxazinyl, phenothiazinyl, pteridinyl, benzothiazolyl, imidazopyridinyl, imidazothiazolyl, dihydrobenzisoxazinyl, benzisoxazinyl, benzoxazinyl, dihydrobenzisothiazinyl, benzopyranyl, benzothiopyranyl, coumarinyl, isocoumarinyl, chromanyl, tetrahydroquinolinyl, dihydroquinolinyl, dihydroquinolinonyl, dihydroisoquinolinonyl, dihydrocoumarinyl, dihydroisocoumarinyl, isoindolinonyl, benzodioxanyl, benzoxazolinonyl, pyrrolyl N-oxide, pyrimidinyl N-oxide, pyridazinyl N-oxide, pyrazinyl N-oxide, quinolinyl N-oxide, indolyl N-oxide, indolinyl N-oxide, isoquinolyl N-oxide, quinazolinyl N-oxide, quinoxalinyl N-oxide, phthalazinyl N-oxide, imidazolyl N-oxide, isoxazolyl N-oxide, oxazolyl N-oxide, thiazolyl N-oxide, indolizinyl N-oxide, indazolyl N-oxide, benzothiazolyl N-oxide, benzimidazolyl N-oxide, pyrrolyl N-oxide, oxadiazolyl N-oxide, thiadiazolyl N-oxide, triazolyl N-oxide, tetrazolyl N-oxide, benzothiopyranyl S-oxide, and benzothiopyranyl S,S-dioxide.
  • Preferred heteroaryl groups include imidazolyl, pyrrolyl, pyridyl, thiazolyl, pyrazolyl, thiazolyl, isoxazolyl, triazolyl, tetrazolyl, oxadiazolyl, pyrimidinyl, and oxazolyl.
  • The term “heterocycloalkyl” is intended to include saturated ring groups having at least 1 heteroatom. Heterocycloalkyl groups typically include 3 to 8 ring atoms, preferably 5 to 7 ring atoms. Heterocycloalkyl groups typically have from 1 to 3 heteroatoms selected from N, S, and O with remaining ring atoms being carbon. Preferably not more than one S atom and one O atom is present in a heterocycloalkyl group. Preferred heterocycloalkyl groups include morpholinyl, piperidinyl, piperazinyl, thiomorpholinyl, and pyrrolidinyl.
  • The phrase “monocyclic or bicyclic ring” refers to saturated, partially unsaturated, or aromatic rings or ring systems, which optionally contain from 1 to 4 heteroatoms independently chosen from N, S, and O with remaining ring members being carbon. Preferred monocyclic and bicyclic rings are saturated and partially unsaturated rings or ring systems.
  • The term “oxo” indicates a carbonyl group. When an oxo group appears as a substituent the allowed valence of the substituted position is not exceeded.
    • Pharmaceutical Compositions
  • The compounds of general Formulas I may be administered orally, topically, parenterally, by inhalation or spray or rectally in dosage unit formulations containing conventional non-toxic pharmaceutically acceptable carriers, adjuvants and vehicles. Oral administration in the form of a pill, capsule, elixir, syrup, lozenge, troche, or the like is particularly preferred. The term parenteral as used herein includes subcutaneous injections, intradermal, intravascular (e.g., intravenous), intramuscular, spinal, intrathecal injection or like injection or infusion techniques. In addition, there is provided a pharmaceutical formulation comprising a compound of general Formula I and a pharmaceutically acceptable carrier. One or more compounds of general Formula I may be present in association with one or more non-toxic pharmaceutically acceptable carriers and/or diluents and/or adjuvants and if desired other active ingredients. The pharmaceutical compositions containing compounds of general Formula I may be in a form suitable for oral use, for example, as tablets, troches, lozenges, aqueous or oily suspensions, dispersible powders or granules, emulsion, hard or soft capsules, or syrups or elixirs.
  • Compositions intended for oral use may be prepared according to any method known to the art for the manufacture of pharmaceutical compositions and such compositions may contain one or more agents selected from the group consisting of sweetening agents, flavoring agents, coloring agents and preserving agents in order to provide pharmaceutically elegant and palatable preparations. Tablets contain the active ingredient in admixture with non-toxic pharmaceutically acceptable excipients that are suitable for the manufacture of tablets. These excipients may be for example, inert diluents, such as calcium carbonate, sodium carbonate, lactose, calcium phosphate or sodium phosphate; granulating and disintegrating agents, for example, corn starch, or alginic acid; binding agents, for example starch, gelatin or acacia, and lubricating agents, for example magnesium stearate, stearic acid or talc. The tablets may be uncoated or they may be coated by known techniques to delay disintegration and absorption in the gastrointestinal tract and thereby provide a sustained action over a longer period. For example, a time delay material such as glyceryl monosterate or glyceryl distearate may be employed.
  • Formulations for oral use may also be presented as hard gelatin capsules wherein the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or as soft gelatin capsules wherein the active ingredient is mixed with water or an oil medium, for example peanut oil, liquid paraffin or olive oil.
  • Aqueous suspensions contain the active materials in admixture with excipients suitable for the manufacture of aqueous suspensions. Such excipients are suspending agents, for example sodium carboxymethylcellulose, methylcellulose, hydropropylmethylcellulose, sodium alginate, polyvinylpyrrolidone, gum tragacanth and gum acacia; dispersing or wetting agents may be a naturally-occurring phosphatide, for example, lecithin, or condensation products of an alkylene oxide with fatty acids, for example polyoxyethylene stearate, or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethyleneoxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and a hexitol such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol anhydrides, for example polyethylene sorbitan monooleate. The aqueous suspensions may also contain one or more preservatives, for example ethyl, or n-propyl p-hydroxybenzoate, one or more coloring agents, one or more flavoring agents, and one or more sweetening agents, such as sucrose or saccharin.
  • Oily suspensions may be formulated by suspending the active ingredients in a vegetable oil, for example arachis oil, olive oil, sesame oil or coconut oil, or in a mineral oil such as liquid paraffin. The oily suspensions may contain a thickening agent, for example beeswax, hard paraffin or cetyl alcohol. Sweetening agents such as those set forth above, and flavoring agents may be added to provide palatable oral preparations. These compositions may be preserved by the addition of an anti-oxidant such as ascorbic acid.
  • Dispersible powders and granules suitable for preparation of an aqueous suspension by the addition of water provide the active ingredient in admixture with a dispersing or wetting agent, suspending agent and one or more preservatives. Suitable dispersing or wetting agents and suspending agents are exemplified by those already mentioned above. Additional excipients, for example sweetening, flavoring and coloring agents, may also be present.
  • Pharmaceutical compositions of the invention may also be in the form of oil-in-water emulsions. The oily phase may be a vegetable oil, for example olive oil or arachis oil, or a mineral oil, for example liquid paraffin or mixtures of these. Suitable emulsifying agents may be naturally-occurring gums, for example gum acacia or gum tragacanth, naturally-occurring phosphatides, for example soy bean, lecithin, and esters or partial esters derived from fatty acids and hexitol, anhydrides, for example sorbitan monoleate, and condensation products of the said partial esters with ethylene oxide, for example polyoxyethylene sorbitan monoleate. The emulsions may also contain sweetening and flavoring agents.
  • Syrups and elixirs may be formulated with sweetening agents, for example glycerol, propylene glycol, sorbitol or sucrose. Such formulations may also contain a demulcent, a preservative and flavoring and coloring agents. The pharmaceutical compositions may be in the form of a sterile injectable aqueous or oleaginous suspension. This suspension may be formulated according to the known art using those suitable dispersing or wetting agents and suspending agents which have been mentioned above. The sterile injectable preparation may also be sterile injectable solution or suspension in a non-toxic parentally acceptable diluent or solvent, for example as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil may be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid find use in the preparation of injectables.
  • The compounds of general Formulas I may also be administered in the form of suppositories, e.g., for rectal administration of the drug. These compositions can be prepared by mixing the drug with a suitable non-irritating excipient that is solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum to release the drug. Such materials are cocoa butter and polyethylene glycols.
  • Compounds of general Formulas I may be administered parenterally in a sterile medium. The drug, depending on the vehicle and concentration used, can either be suspended or dissolved in the vehicle. Advantageously, adjuvants such as local anesthetics, preservatives and buffering agents can be dissolved in the vehicle.
  • For administration to non-human animals, the composition may also be added to the animal feed or drinking water. It will be convenient to formulate these animal feed and drinking water compositions so that the animal takes in an appropriate quantity of the composition along with its diet. It will also be convenient to present the composition as a premix for addition to the feed or drinking water.
  • Dosage levels of the order of from about 0.1 mg to about 140 mg per kilogram of body weight per day are useful in the treatment of the above-indicated conditions (about 0.5 mg to about 7 g per patient per day). The amount of active ingredient that may be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated and the particular mode of administration. Dosage unit forms will generally contain between from about 1 mg to about 500 mg of an active ingredient.
  • Frequency of dosage may also vary depending on the compound used and the particular disease treated. However, for treatment of most disorders, a dosage regimen of 4 times daily or less is preferred. For the treatment of anxiety, depression, or cognitive impairment a dosage regimen of 1 or 2 times daily is particularly preferred. For the treatment of sleep disorders a single dose that rapidly reaches effective concentrations is desirable.
  • It will be understood, however, that the specific dose level for any particular patient will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, and rate of excretion, drug combination and the severity of the particular disease undergoing therapy.
  • Preferred compounds of the invention will have certain pharmacological properties. Such properties include, but are not limited to high solubility (preferably 500 ng/ml or more) in aqueous solutions, oral bioavailability, low toxicity, low serum protein binding, lack of clinically relevant EKG effects, and desirable in vitro and in vivo half-lifes. Penetration of the blood brain barrier for compounds used to treat CNS disorders is necessary, while low brain levels of compounds used to treat periphereal disorders are often preferred.
  • Assays may be used to predict these desirable pharmacological properties. Assays used to predict bioavailability include transport across human intestinal cell monolayers, including Caco-2 cell monolayers. Toxicity to cultured hepatocycles may be used to predict compound toxicity. Penetration of the blood brain barrier of a compound in humans may be predicted from the brain levels of the compound in laboratory animals given the compound intravenously.
  • Serum protein binding may be predicted from albumin binding assays. Such assays are described in a review by Oravcová, et al. (Journal of Chromatography B (1996) volume 677, pages 1-27).
  • Compound half-life is inversely proportional to the frequency of dosage of a compound. In vitro half-lifes of compounds may be predicted from assays of microsomal half-life as described by Kuhnz and Gieschen (Drug Metabolism and Disposition, (1998) volume 26, pages 1120-1127).
    • EXAMPLES Preparation of Compounds
  • Representative procedures suitable for the preparation of compounds of Formula I are outlined in Schemes I-X, which are not to be construed as limiting the invention in scope or spirit to the specific reagents and conditions shown in them. Those having skill in the art will recognize that the reagents and conditions may be varied and additional steps employed to produce compounds encompassed by the present invention. In some cases, protection of reactive functionalities may be necessary to achieve the desired transformations. In general, such need for protecting groups, as well as the conditions necessary to attach and remove such groups, will be apparent to those skilled in the art of organic synthesis. Unless otherwise stated in the schemes below, the variables, e.g., Z1, Z2, Z3, Z4, R5, R2, R3 and W, are as defined in Formula I.
  • Figure US20100267698A1-20101021-C00021
  • Scheme I illustrates a route to selected compounds of Formula 6 via coupling of chloromethyl compounds 4 and aryl imidazoles 5. In Step 1, aryl and heteroaryl halides of formula 1 are reacted with appropriate amines in the presence of base to obtain amino adducts of formula 2. In Step 2, reduction of the nitro group in compounds of formula 2 yields diamines 3. In Step 3, diamines of formula 3 are reacted with 2-chloro-acetimidic acid methyl ester hydrochloride or a similar electrophile such as 2-chloro-1,1,1-trimethoxy-ethane or chloroacetic acid anhydride. In Step 4, chloromethyl compounds of formula 4 are reacted with aryl and heteroaryl imidazoles of formula 5 in the presence of base and solvent to obtain compounds of formula 6. Depending on the particular nature of 5, a stronger or weaker base may be selected to facilitate the reaction in Step 4.
  • Figure US20100267698A1-20101021-C00022
  • Scheme II illustrates the synthesis of compounds of formula 10 from diamines 3. In Step 1, reaction of malonyl dichloride with ethyl vinyl ether provides 7. In Step 2, treatment of 7 with triethyl orthoformate in the presence of acid yields 8. Compound 8 is reacted in Step 3 with a variety of aryl and heteroaryl hydrazines to obtain compounds of formula 9 as a mixture with the undesired regioisomer. As illustrated in Steps 4 and 5, compounds of formula 9 can be hydrolyzed to the corresponding acids and coupled with compounds of formula 3 to obtain, following cyclization in refluxing acetic acid, compounds of formula 10. As described in subsequent schemes and examples, compounds of formula 9 may also be directly coupled to compounds of formula 3 in the presence of trimethylaluminum. Depending on the particular example and reaction conditions selected, cyclization may occur without need for heating in acetic acid as described in Step 5.
  • Figure US20100267698A1-20101021-C00023
    Figure US20100267698A1-20101021-C00024
  • Scheme III illustrates a method for preparing compounds of formula 21 and 22. Step 1 encompasses hydrolysis of compounds of formula 11 to the corresponding acids followed by dimerization in the presence of a suitable coupling reagent such as 1,1′-carbonyldiimidazole to form compounds of formula 12. In Step 2, deacylation of compounds of formula 12 is accomplished by heating with concentrated sulfuric acid to obtain compounds of formula 13. Heating of compounds of formula 13 with ammonium hydroxide in Step 3 results in formation of compounds of formula 14. Nitration of compounds of formula 14 in Step 4 is accomplished using nitric acid to obtain compounds of formula 15. Compounds of formula 15 are converted to the corresponding chlorides 16 in Step 5 by heating with phosphorous oxychloride. In Step 6, chlorides 16 are reacted with ammonia followed by heating with phosphorous oxychloride to obtain 2-chloropyridines 17, which are subsequently reduced to diamines 18 in Step 7. In Step 8, diamines 18 are reacted with esters of formula 19 in the presence of trimethylaluminum followed by heating in acetic acid to obtain compounds of formula 20. Depending on the particular example and reaction conditions selected, cyclization may occur without need for heating in acetic acid. Step 9 illustrates alkylation of compounds of formula 20 with ethyl iodide in the presence of base to obtain a mixture of compounds of formula 21 and 22. Those skilled in the art will realize that alternate alkylating agents may be employed to obtain similar compounds bearing different R5 groups.
  • Figure US20100267698A1-20101021-C00025
  • Scheme IV illustrates a variation of Scheme III for specifically preparing compounds of formula 22. In Step 1, compounds of formula 16 are reacted with ethyl amine to form the amino adducts which are subsequently converted to 2-chloropyridines 23 by reaction with phosphorous oxychloride. Those skilled in the art will realize that numerous other suitable amines of formula R5NH2 may be employed in Step 1 to yield other variants of Formula I. In Steps 2 and 3, hydrogenation of compounds of formula 23 to diamines of formula 24 followed by trimethylaluminum-facilitated coupling and cyclization in acetic acid provides compounds of formula 22. Depending on the particular example and reaction conditions selected, cyclization may occur without need for heating in acetic acid
  • Figure US20100267698A1-20101021-C00026
  • Scheme V illustrates a route employing a protecting group strategy for preparing pyrazole compounds of formula 29. In Step 1, pyrazole 25 is reacted with di-tert-butyldicarbonate in the presence of 4-dimethylaminopyridine to obtain 26. Reaction with glyoxal and ammonium hydroxide provides 27. Reaction of 27 with chloromethyl compounds of formula 4 in the presence of base provides compounds of formula 28. Deprotection of compounds of formula 28 with acid in Step 4 provides pyrazoles of formula 29.
  • Figure US20100267698A1-20101021-C00027
  • Scheme VI provides a route for preparing thiazole compounds of formula 33. Step 1 involves bromination of ketoesters of formula 30 to form α-bromoketones of formula 31. In Step 2, compounds of formula 31 are reacted with thioformamide to obtain thiazoles of formula 32. Condensation of 32 with 3 in Step 3 in the presence of trimethylaluminum provides compounds of formula 33. Depending on the particular reactants and conditions employed in Step 3, the product mixture may require heating in a suitable solvent such as acetic acid to enhance formation of 33.
  • Figure US20100267698A1-20101021-C00028
  • Scheme VII provides routes to several heterocyclic systems via common intermediate 35. In Step X, compounds of formula 34 are reacted with compounds of formula 4 at low temperature in the presence of a suitable base such as lithium diisopropylamide to form compounds of formula 35. R×n 1 illustrates conversion of ketones of formula 35 to isoxazole derivatives of formula 36 by reaction of compounds of formula 35 with tris(dimethylamino)methane followed by treatment with hydroxylamine. In R×n 2, compounds of formula 35 are reacted with tris(dimethylamino)methane followed by treatment with hydrazine acetate to obtain pyrazoles 37. In R×n 3 and 4, compounds of formula 35 are brominated to form α-bromoketones 38 that are subsequently reacted with thioformamide to obtain thiazoles of formula 39. R×n 5 illustrates the synthesis of pyrazoles of formulas 40 and 41 by reaction of compounds of formula 35 with tris(dimethylamino)methane followed by treatment with methyl hydrazine.
  • Figure US20100267698A1-20101021-C00029
  • Scheme VIII provides a route for preparation of tetrazoles of formula 44. In Step 1, aryl and heteroaryl tetrazoles of formula 42 are heated with bis(tributyltin) oxide to form stannanes of formula 43. In Step 2, heating compounds of formula 43 with compounds of formula 4 in a suitable solvent such as toluene gives compounds of formula 44.
  • Figure US20100267698A1-20101021-C00030
  • Scheme IX illustrates the synthesis of triazoles of formula 50. In Step 1, aryl and heteroaryl hydrazines are reacted with 1,3,5-triazine 45 to obtain triazoles of formula 46. In Step 2, heating compounds of formula 46 with formaldehyde provides alcohols of formula 47. In Step 31 alcohols of formula 47 are converted to the corresponding chlorides by treatment with thionyl chloride. The chlorides are subsequently converted to nitrites 48 by the action of tetraethyl ammonium cyanide. Cyanides 48 are hydrolyzed in Step 4 to carboxylic acids 49. In Step 5, carboxylic acids of formula 49 are coupled with diamines 3 in the presence of EDCI [1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride] or other suitable coupling reagents followed by heating in acetic acid to complete cyclization of the intermediate amino amides to compounds of formula 50.
  • Figure US20100267698A1-20101021-C00031
  • Scheme X illustrates two routes for the synthesis of imidazoles of formula 52, which are intermediates in the synthesis of selected compounds of Formula I. In Step 1, aryl and heteroaryl aldehydes are treated with glyoxal and ammonium hydroxide to form imidazoles of formula 52. In Step 1′, imidazole 53 is treated with butyl lithium followed by tri-n-butyltin chloride to obtain compounds of formula 54, which must be handled with care to avoid decomposition. In Step 2′, compounds of formula 54 are utilized in palladium cross-coupling reactions with aryl and heteroaryl halides to obtain compounds of formula 55. Subsequent treatment of compounds of formula 55 with acid in Step 3′ provides compounds of formula 52.
  • The invention is illustrated further by the following examples, which are not to be construed as limiting the invention in scope or spirit to the specific procedures described in them. Additional compounds encompassed by this invention beyond the accompanying Examples may be prepared using methods known to those skilled in the art of chemical synthesis. Those having skill in the art will recognize that the starting materials may be varied and additional steps employed to produce compounds encompassed by the present inventions, as demonstrated by the following examples. In some cases, protection of reactive functionalities may be necessary to achieve some the desired transformations. In general, such need for protecting groups, as well as the conditions necessary to attach and remove such groups, will be apparent to those skilled in the art of organic synthesis.
    • Preparation of Starting Materials and Intermediates
  • The starting materials and various intermediates may be obtained from commercial sources, prepared from commercially available organic compounds, or prepared using known synthetic methods. Representative examples of methods suitable for preparing intermediates of the invention are set forth below.
    • Example 1 Synthesis of 1-Propyl-2-{[2-(2-fluoropyrid-6-yl)-1H-imidazol-1-yl]methyl}-5-cyano-1H-benzimidazole
  • Figure US20100267698A1-20101021-C00032
    • 1. Preparation of 4-n-Propylamino-3-nitrobenzonitrile
  • To a stirring suspension of 4-chloro-3-nitrobenzonitrile (7.30 g, 40 mmol) in isopropanol (30 mL) is added n-propylamine (9.87 mL, 120 mmol). The mixture is stirred at room temperature for 5 h, and the solid is then collected by filtration to give 4-n-propylamino-3-nitrobenzonitrile as a yellow solid. 1H NMR (CDCl3) δ 8.51 (1H, q), 8.42 (1H, br s), 7.60 (1H, m), 6.91 (1H, d), 3.33 (2H, q), 1.84-1.74 (2H, m), 1.07 (3H, t). LRMS 206.3 (MH+).
    • 2. Preparation of 3-Amino-4-n-propylaminobenzonitrile
  • To a Parr bottle containing 4-n-propylamino-3-nitrobenzonitrile (7.63 g, 37.2 mmol) in ethyl acetate (38 mL) is added 5% Pd/C (50% wet, 633 mg). The Parr bottle is sealed in a mechanical shaker, evacuated, and then purged with nitrogen followed by hydrogen. The system is pressurized to 50 PSI of hydrogen at room temperature and mechanical shaking engaged. After 2 hours, shaking is stopped, and the system purged with nitrogen prior to opening the vessel. The reaction mixture is filtered through celite, concentrated in vacuo, and the obtained solid recrystallized by dissolving in ethyl acetate (15 mL), heating, and adding hexanes (15 mL), to give 3-Amino-4-n-propylaminobenzonitrile as gray crystals. 1H NMR (CDCl3) δ 7.14 (1H, dd), 6.92 (1H, d), 6.56 (1H, d), 3.98 (1H, br s), 3.30 (2H, br s), 3.12 (2H, t), 1.75-1.65 (2H, m), 1.03 (3H, t). LRMS calcd 175.23. found 176.2 (MH+).
    • 3. Preparation of 1-Propyl-2-{[2-(2-fluoropyrid-6-yl)-1H-imidazol-1-yl]methyl}-5-cyano-1H-benzimidazole Method A (A) Preparation of 1-n-Propyl-2-chloromethyl-5-cyanobenzimidazole hydrochloride
  • i) A solution of 3-amino-4-n-propylaminobenzonitrile (7.38 g, 42.1 mmol) and ethyl chloroacetimidate hydrochloride (9.92 g, 63.2 mmol) in ethanol (100 mL) is heated at reflux for 17 h, then cooled and concentrated to give 1-n-Propyl-2-chloromethyl-5-cyanobenzimidazole hydrochloride. Prior to use in the next step, this material is converted to free base by adding aqueous bicarbonate and extracting with dichloromethane, drying (Na2SO4), and concentrating.
  • ii) Alternatively, chloroacetylchloride rather than ethyl chloroacetimidate can by used: To a solution of 3-amino-4-n-propylaminobenzonitrile (5.15 g, 29.4 mmol) and triethylamine (4.51 mL) in ethyl acetate (52 mL) at room temperature, chloroacetyl chloride (2.57 mL) is added slowly. After stirring the reaction mixture for 30 minutes at room temperature, acetic acid (5 mL) is added and the reaction mixture heated to reflux. After heating for 20 h, the reaction mixture is cooled to room temperature and diluted with water (50 mL). The organic solution is washed twice with 1.0 M sodium hydroxide (2×50 mL), then washed with an aqueous solution of 0.25 M KH2PO4 (50 mL), followed by brine (50 mL). The organic layer is dried (sodium sulfate), concentrated in vacuo, and the solid recrystallized by heating in ethyl acetate (20 mL), adding hexane (40 mL) and cooling to room temperature with stirring to afford 1-n-Propyl-2-chloromethyl-5-cyanobenzimidazole as brown crystals. 1H NMR (CDCl3): δ 8.08 (d, J=0.8 Hz, 1H), 7.57 (dd, J=1.65, 8.52 Hz, 1H), 7.45 (d, J=8.24 Hz, 1H), 7.26 (s, 1H), 4.84 (s, 2H) 4.24 (t, J=7.6 Hz, 2H), 1.94 (pentet, J=7.4. 7.6 Hz, 2H), 1.03 (t, S=7.4 Hz, 3H).
    • (B) Preparation of 1-Propyl-2-{[2-(2-fluoropyrid-6-yl)-1H-imidazol-1-yl]methyl}-5-cyano-1H-benzimidazole
  • To a stirring suspension of sodium hydride (2.25 g of 60% in oil) in DMF (10 mL) at 0° a solution of 2-Fluoro-6-(1H-imidazol-2-yl)-pyridine (7.7 g, 47.2 mmol) in DMF (20 mL) is added. After stirring for 5 minutes, a solution of 1-n-Propyl-2-chloromethyl-5-cyanobenzimidazole (11 g, 47.2 mmol) and sodium iodide (20 mg) in DMF (80 mL) is added. The reaction mixture is stirred for 6 h, gradually warmed to room temperature. The reaction mixture is cooled, water added, the solid collected, rinsed with water and dried to afford the title compound, 1-Propyl-2-{[2-(2-fluoropyrid-6-yl)-1H-imidazol-1-yl]methyl}-5-cyano-1H-benzimidazole.
  • Method B
  • Trimethylaluminum (0.54 mL of 2.0M in toluene, 1.09 mmol) is added dropwise to a solution of 3-Amino-4-propylaminobenzonitrile (152 mg; 0.87 mmol) in dichloromethane (10 mL), and the mixture was stirred at room temperature for 1 hr. A solution of [2-(2-Fluoropyridin-6-yl)-imidazol-1-yl]acetic acid methyl ester (102 mg; 0.43 mmol) in dichloromethane (5 mL) is added all at once, and the mixture heated at reflux for 16 hr. The brown solution is cooled to room temperature and treated dropwise with methanol (1 mL) then water (2 mL) and stirred at room temperature for 15 min. Anhydrous sodium sulfate is added until the gel becomes solid, the mixture is diluted with dichloromethane (100 mL) and filtered through celite. The filtrate is concentrated to give a brown oil which is dissolved in acetic acid (7 mL) and heated at 100° for 72 hr. The mixture is cooled to room temperature and concentrated. The residue is dissolved in ethyl acetate (15 mL), washed with saturated aqueous NaHCO3 (1×50 mL), then brine (1×50 mL), dried (MgSO4), and concentrated, and the residue purified by preparative thin layer chromatography to give 1-n-Propyl-2-{[2-(2-fluoropyrid-6-yl)-1H-imidazol-1-yl]methyl}-5-cyano-1H-benzimidazole as a light brown semi-solid (57 mg).
    • Method C
  • A solution of [2-(6-Fluoropyridin-2-yl)-imidazol-1-yl]acetic acid hydrochloride (274 mg; 1.06 mmol) and triethylamine (0.15 mL; 1.06 mmol) in dichloromethane (10 mL) is treated dropwise with oxalyl chloride (0.64 mL of a 2M solution in dichloromethane; 1.27 mmol), and the resulting suspension stirred at room temperature for 2 hr.
  • The mixture is concentrated and the residue suspended in dichloromethane (10 mL). A solution of 3-Amino-4-propylaminobenzonitrile (185 mg; 1.06 mmol) in dichloromethane (5 mL) is added and the mixture stirred at room temperature for 16 hr, then concentrated. The residue is dissolved in acetic acid (10 mL) and heated at 1000 for 1 hr. The mixture is cooled to room temperature, concentrated, taken up in ethyl acetate (150 mL), washed with saturated aqueous NaHCO3 (50 mL) and then washed with brine (50 mL), dried (MgSO4), and concentrated to give the crude product as a waxy brown solid (338 mg). This crude product is slurried with diethyl ether (ca. 4 mL) and a few drops of methanol, then filtered to give 1-n-Propyl-2-{[2-(2-fluoropyrid-6-yl)-1H-imidazol-1-yl]methyl}-5-cyano-1H-benzimidazole as a pale brown solid (230 mg).
  • 1H NMR (399.96 MHz, CDCl3): δ 8.17 (dd, J=2.0, 7.6 Hz, 1H, H-18), 8.05 (s, 1H, H-4), 7.88 (q, J=8.0 Hz, 1H, H-19), 7.52 (d, J=8.4 Hz, 1H, H-6), 7.41 (d, J=8.4 Hz, 1H, H-7), 7.21 (s, 1H, H-14), 7.18 (s, 1H, H-15), 6.28 (s, 2H, H-13), 4.28 (t, J=7.6 Hz, 2H, H-10), 1.68 (dt, J=7.6 Hz, 2H, H-11), 0.84 (t, J=7.6 Hz, 3H, H-12).
  • 13C NMR (100.57 MHz, CDCl3, 1H decoupled at 399.957 MHz): δ, 162.36 (d, JC-F=239.6 Hz), 152.53, 148.69 (d, JC-F=13.0 Hz), 142.36 (d, JC-F=7.6 Hz), 142.31, 142.28, 138.42, 129.96, 126.55, 125.31, 124.23, 120.27 (d, JC-F=3.8 Hz), 119.96, 111.23, 108.52 (d, JC-F=35.9 Hz), 105.70, 46.10, 44.57, 23.43, 11.27.
    • Example 2 Synthesis of 1-Ethyl-2-{[2-(3-fluorophenyl)-pyrazol-3-yl]methyl}-5-cyano-1H-benzimidazole
  • Figure US20100267698A1-20101021-C00033
    • 1. Preparation of 1-(3-Fluorophenyl)-5-carboxymethylpyrazole
  • To a stirring solution of ethyl vinyl ether (340 ml, 3.55 mol) in diethyl ether (200 mL), cooled in an ice bath, is added dropwise a solution of malonyl dichloride (69 mL, 0.71 mol) in ether (20 mL). Stirring is continued at 0° C. for 2 h, then a solution of triethylamine (196 mL) and ethanol (350 mL) in ether (210 mL) is added with cooling. More ether is added to further precipitate triethylamine hydrochloride, the mixture is then filtered, and the filtrate concentrated. The residue is taken up in ethanol (710 mL), then triethyl orthoformate (177 mL, 1.07 mol) and concentrated HCl (5 mL) is added. The mixture is stirred overnight then concentrated. To the residue is added ethanol (500 mm) and 3-Fluorophenylhydrazine hydrochloride (27.6 g, 0.17 mol). The mixture is heated at reflux for 2 h, then cooled and concentrated. Ethyl acetate is added, the mixture is washed with aqueous bicarbonate, then with water, dried (Na2SO4), concentrated, and the residue purified by silica gel chromatography. Ethanol (20 mL) and 1 N NaOH (100 mL) are added, and heated at reflux for 1 h. The mixture is cooled and washed with ethyl acetate. The aqueous layer is cooled and acidified, the solid collected by filtration, rinsed well with water and dried to give 1-(3-Fluorophenyl)-5-carboxymethylpyrazole as a gold solid. 1H NMR (CDCl3): δ 7.68 (d, J=1.8 Hz, 1H), 7.41-7.48 (m, 1H), 7.10-7.26 (m, 3H), 6.44 (d, J=2.1 Hz, 1H), 3.77 (s, 2H). LCMS: 221.2 (MH+), 219.2 (MH−).
    • 2. Preparation of 1-Ethyl-2-{[2-(3-fluorophenyl)-pyrazol-3-yl]methyl}-5-cyano-1H-benzimidazole
  • To a stirring solution of 3-amino-4-ethylaminobenzonitrile (887 mg, 5.5 mmol) and 1-(3-Fluorophenyl)-5-carboxymethylpyrazole (1.10 g, 5 mmol) in pyridine (5 mL) is added 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (1.15 g, 6 mmol). The mixture is stirred at room temperature for 17.5 h, then concentrated. The residue is cooled in an ice bath, aqueous HCl is added with stirring, the precipitate is collected by filtration, rinsed well with water and dried. The solid is added to acetic acid (75 mL) and heated at reflux for 5.5 h. The mixture is cooled, concentrated, and purified by silica gel chromatography to give 1-Ethyl-2-{[2-(3-fluorophenyl)-pyrazol-3-yl]methyl}-5-cyano-1H-benzimidazole. The product is converted to the mesylate salt in acetone. 1H NMR (CDCl3): δ 8.17 (s, 1H), 7.89 (d, J=8.52 Hz, 1H), 7.68-7.75 (m 1H), 7.44-7.54 (m, 2H), 7.37-7.40 (m, 1H), 7.21-7.28 (m, 1H), 6.35 (d, J=1.65 Hz, 1H), 4.65 (s, 2H), 4.29 (q, J=7.14 Hz, 2H), 2.34 (s, 3H), 1.19 (t, J=7.14 Hz, 3H). LCMS 346.0 (MH+), 344.4 (MH−).
    • Example 3 Synthesis of 1-Ethyl-2-{[1-(3-fluorophenyl)-1,2,4-triazol-5-yl]methyl}-5-cyano-1H-benzimidazole
  • Figure US20100267698A1-20101021-C00034
    • 1. Preparation of 1-(3-Fluorophenyl)-1,2,4-triazole
  • A mixture of 1,3,5-triazine (1 g, 12.3 mmol) and 3-Fluorophenylhydrazine hydrochloride (2 g, 12.3 mmol) in ethanol (20 mL) is heated at reflux overnight. After concentrating, ethyl acetate is added, the mixture is washed with aqueous bicarbonate followed by saturated aqueous sodium chloride, dried (MgSO4), and concentrated to give 1.8 g of crude 1-(3-Fluorophenyl)-1,2,4-triazole. 1H NMR (CDCl3): δ 8.57 (s, 1H), 8.12 (s, 1H), 7.46-7.51 (m, 3H), 7.08-7.15 (m, 1H). LCMS 164.1 (MH+).
    • 2. Preparation of 1-(3-Fluorophenyl)-5-hydroxymethyl-1,2,4-triazole
  • A mixture of the crude 1-(3-Fluorophenyl)-1,2,4-triazole and formaldehyde (10 mL of 37 wt % in water) is heated at 150° C. in a sealed tube for 48 h. After cooling the reaction vessel, the reaction mixture is extracted with dichloromethane, dried (MgSO4), concentrated, and the residue purified using silica gel chromatography to afford 1-(3-Fluorophenyl)-5-hydroxymethyl 1,2,4-triazole. 1H NMR (CDCl3): δ 8.02 (s, 1H), 7.42-7.53 (m, 3H), 7.22-7.29 (m, 1H), 4.81 (s, 2H). LCMS 194.2 (MH−).
    • 3. Preparation of 1-(3-Fluorophenyl)-5-cyanomethyl-1,2,4-triazole
  • 1-(3-Fluorophenyl)-5-hydroxymethyl-1,2,4-triazole (1.7 g) is treated with thionyl chloride (10 mL) in dichloromethane (20 mL) at room temperature overnight. The solvent is then removed. To the residue is added acetonitrile (20 mL), tetraethylammonium cyanide (2.75 g, 17.6 mmol), and triethylamine (2.5 mL, 17.6 mmol). The mixture is stirred at room temperature for 2 h. The mixture is diluted with ethyl acetate, washed with aqueous bicarbonate, and then with saturated aqueous sodium chloride, dried (Na2SO4), concentrated and the residue purified by silica gel chromatography to give crude 1-(3-Fluorophenyl)-5-cyanomethyl-1,2,4-triazole. 1H NMR (CDCl3): δ 8.06 (s, 1H), 7.52-7.62 (m, 1H), 7.20-7.30 (m, 3H), 3.98 (s, 2H). LCMS 203.0 (MH+), 201.2 (MH−).
    • 4. Preparation of 1-(3-Fluorophenyl)-5-carboxymethyl-1,2,4-triazole
  • Crude 1-(3-Fluorophenyl)-5-cyanomethyl-1,2,4-triazole (0.9 g) is taken up in ethanol (50 mL) and cooled in an ice bath. HCl gas is bubbled through for 0.5 h. Water (10 mL) is added and the mixture heated at 65° C. for 2 h. After cooling, most of the ethanol is removed on a roto-evaporator, 3 N NaOH (25 mL) and ethanol (25 mL) are then added. The solution is heated at reflux for 2 h, cooled, and then extracted with diethyl ether (3×). The aqueous layer is acidified to pH 2, extracted with ethyl acetate. The organic layer is washed with saturated aqueous sodium chloride, dried (Na2SO4), and concentrated to give 1-(3-Fluorophenyl)-5-carboxymethyl-1,2,4-triazole. 1H NMR (CDCl3): δ 8.08 (s, 1H), 7.50-7.57 (m, 1H), 7.22-7.32 (m, 3H), 4.01 (s, 2H).
    • 5. Preparation of 1-Ethyl-2-{[1(3-fluorophenyl)-1,2,4-triazol-5-yl]methyl}-5-cyano-1H-benzimidazole
  • Using the procedure described in Example 2, Step 2, 1-(3-fluorophenyl)-5-carboxymethyl-1,2,4-triazole is converted to 1-Ethyl-2-{[1-(3-fluorophenyl)-1,2,4-triazol-5-yl]methyl}-5-cyano-1H-benzimidazole. 1H NMR (CDCl3): δ 8.03 (s, 1H); 8.01 (s, 1H); 7.42-7.56 (m, 5H); 7.22 (m, 1H); 4.51 (s, 2H); 4.43 (q, 2H); 1.45 (t, 3H). LCMS 347.3 (MH+).
    • Example 4 Synthesis of 1-Propyl-2-[(5-phenyl-1H-tetrazol-1-yl)methyl]-1H-benzimidazole
  • Figure US20100267698A1-20101021-C00035
    • 1. Preparation of 2-(Tri-n-butyltin)-5-phenyl-1 h-tetrazole
  • A mixture of 5-phenyl-1H-tetrazole (200 mg, 1.22 mmol) and bis(tri-n-butyltin)oxide (0.31 mL, 0.61 mmol) in ethanol (2 mL) is heated at reflux for 10 minutes. The mixture is cooled, concentrated, and the crude 2-(Tri-n-butyltin)-5-phenyl-1H-tetrazole used directly.
    • 2. Preparation of 1-Propyl-2-[5-phenyl-1H-tetrazol-1-yl)methyl]-1H-benzimidazole
  • Crude 2-(tri-n-butyltin)-5-phenyl-1H-tetrazole (2.09 g, 4.8 mmol) and 1-propyl-2-chloromethylbenzimidazole (1.0 g, 4.8 mmol) are heated in toluene (5 mL) at reflux overnight. The solvent is removed in vacuo, the residue washed with hexane, and then purified by preparative chromatography to give 1-Propyl-2-[(5-phenyl-1H-tetrazol-1-yl)methyl]-1H-benzimidazole as the minor isomer. 1H NMR (CDCl3): δ 8.05-8.12 (m, 2H); 7.77 (m, 1H); 7.58-7.62 (m, 2H); 7.29-7.43 (m, 3H); 5.86 (5, 2H); 4.40 (t, 2 h); 1.85 (m, 2H); 1.01 (t, 3H).
    • Example 5 Synthesis of 1-Ethyl-2-{[2-(3-fluorophenyl)-pyrazol-3-yl]methyl}-5-acetyl-1H-benzimidazole
  • Figure US20100267698A1-20101021-C00036
  • A mixture of 1-Ethyl-2-{[2-(3-fluorophenyl)-pyrazol-3-yl]methyl}-5-bromo-1H-benzimidazole (200 mg, 0.5 mmol), tributyl(1-ethoxyvinyl)tin (0.34 ml, 1.0 mmol), and tetrakis(triphenylphosphine)palladium(0) (29 mg) in toluene (10 mL) is heated at reflux for 1 h under argon. The solvent is removed in vacuo, the residue is then dissolved in 10% HCl (5 mL) and THF (5 mL). The mixture is stirred at room temperature for 0.5 h, then extracted with ethyl acetate. The aqueous layer is adjusted to pH 9, extracted with dichloromethane, and the organic layer dried (Na2SO4), concentrated, and the residue purified by preparative silica gel chromatography to give 1-Ethyl-2-{[2-(3-fluorophenyl)-pyrazol-3-yl]methyl}-5-acetyl-1H-benzimidazole. 1H NMR (CDCl3): δ 8.36 (d, 1H); 7.99 (dd, 1H); 7.645 (d, 1H); 7.25-7.49 (m, 4H); 7.14 (m, 1H); 6.21 (d, 1 h); 4.36 (s, 2H); 4.02 (g, 2H); 2.68 (s, 3H); 1.24 (t, 3H).
    • Example 6 Synthesis of 1-Ethyl-2-{[2-(3-fluorophenyl)-pyrazol-3-yl]methyl}-5-[1-(methoxyimino)ethyl]-1H-benzimidazole
  • Figure US20100267698A1-20101021-C00037
  • A mixture of 1-Ethyl-2-{[2-(3-fluorophenyl)-pyrazol-3-yl]methyl}-5-acetyl-1H-benzimidazole (23 mg), methoxylamine hydrochloride (15 mg, 3 eq.), and sodium acetate (15 mg, 3 eq.) in methanol (1 mL) is stirred at room temperature overnight. The solvent is removed in vacuo and aqueous sodium bicarbonate added to pH 9, then extracted with ethyl acetate. The organic layer is dried (Na2SO4) and concentrated to give 1-Ethyl-2-{[2-(3-fluorophenyl)-pyrazol-3-yl]methyl}-5-[1-(methoxyimino)ethyl]-1H-benzimidazole. 1H NMR (CDCl3): δ 7.94 (d, 1H); 7.73 (dd 1H); 7.73 (dd, 1H); 7.63 (d, 1H); 7.52 (m, 1H); 7.38-7.40 (m, 3H); 7.27 (m, 1H); 6.19 (d, 1H); 4.32 (s, 2H); 3.87-4.10 (m, 5H); 2.30 (s, 3H); 1.22 (t, 3H).
    • Example 7 Synthesis of 1-Ethyl-2-{[2-(2,5-difluorophenyl)-pyrazol-3-yl]methyl}-5-[5-(4-fluorophenyl)-1,3,4-oxadiazol-2-yl]-1H-benzimidazole
  • Figure US20100267698A1-20101021-C00038
  • A mixture of 1-Ethyl-2-{[2-(2,5-difluorophenyl)-pyrazol-3-yl]methyl}-1H-benzimadol-5-carboxylate (152 mg) and 4-fluorobenzhydrazide (1.05 eq.) in phosphorous oxychloride (6 mL) is heated at reflux for 1.5 h. The mixture is cooled and concentrated, and then water (5 mL) is added to the residue. After adjusting to pH >7 with saturated aqueous bicarbonate, the solution is extracted with ethyl acetate (3×), and the combined organic layers are washed with water (2×) then with saturated aqueous sodium chloride, dried (MgSO4), concentrated, and the residue purified by preparative silica gel chromatography to give 1-Ethyl-2-{[2-(2,5-difluorophenyl)-pyrazol-3-yl]methyl}-5-[5-(4-fluorophenyl)-1,3,4-oxadiazol-2-yl]-1H-benzimidazole. LCMS MH+ 501.068.
    • Example 8 Synthesis of 1-Ethyl-2-{[2-(3-fluorophenyl)-1H-imidazol-1-yl]methyl}-5-(1,3,4-oxadiazol-2-yl)-1H-benzimidazole
  • Figure US20100267698A1-20101021-C00039
  • A mixture of 1-ethyl-2-{[2-(3-fluorophenyl)-1H-imidazol-1-yl]methyl}-1H-benzimadol-5-hydrazide (177 my), triethylorthoformate (8 mL) and acetic acid (2 mL) is heated at reflux for 5 h. The reaction is cooled, concentrated, and the residue purified by preparative silica gel chromatography to give 1-Ethyl-2-{[2-(3-fluorophenyl)-1H-imidazol-1-yl]methyl}-5-(1,3,4-oxadiazol-2-yl)-1H-benzimidazole, which was converted to the hydrochloride salt in ethyl acetate. 1H NMR (d6 DMSO): δ 9.32 (s, 1H), 8.19 (s, 1H), 8.03 (d, 1H), 7.97-7.95 (m, 2Hm), 7.87 (d, 1H), 7.72 (m, 1H), 7.66-7.60 (m, 2H), 7.54-7.50 (m, 1H), 5.99 (s, 2H), 4.34 (q, 2H), 1.31 (t, 3H), LCMS MH+ 389.4
    • Example 9 Synthesis of 1-Ethyl-2-{[2-(2,5-difluorophenyl)-1H-imidazol-1-yl]methyl}-5-amino-1H-benzimidazole
  • Figure US20100267698A1-20101021-C00040
    • 1. Preparation of 4-Fluoro-3-nitroacetanilide
  • 4-Fluoro-3-nitroaniline (5.2 g) is treated with acetic anhydride (1.1 eq.) in dichloromethane at room temperature for 1 h. The reaction is concentrated, the residue taken up in dichloromethane and washed with aqueous sodium bicarbonate (2×) then with saturated aqueous sodium chloride, dried (MgSO4), and concentrated to give 4.7 g of crude 4-Fluoro-3-nitroacetanilide.
    • 2. Preparation of 3-Nitro-4-(ethylamino)acetanilide
  • A mixture of crude 4-Fluoro-3-nitroacetanilide (4.6 g), ethylamine (23 mL of 2M in THF), and potassium carbonate (3.5 g) in DMF (100 mL) is stirred at room temperature for 4 h, then heated at 60° C. for 5 h. The reaction is allowed to cool, water (150 mL) is added, and extracted with ethyl acetate (3×). The combined organic extracts are washed with water (3×), then washed with saturated aqueous sodium chloride, dried (MgSO4), and concentrated to give crude 3-Nitro-4-(ethylamino)acetanilide.
    • 3. Preparation of N-[3-Amino-4-(ethylamino)phenyl]acetamide
  • Crude 3-Nitro-4-(ethylamino)acetanilide (4.8 g), 10% Pd/C (0.5 g), methanol (50 mL) and ethyl acetate (200 mL) are placed in a Paar apparatus under hydrogen at 50 psi for 5 h. The mixture is filtered through Celite and concentrated to give 4 g of crude N-[3-Amino-4-(ethylamino)phenyl]acetamide
    • 4. Preparation of N-{2-{[2-(2,5-Difluorophenyl)-1H-imidazol-1-yl]methyl}-1-ethyl-1H-benzimidazol-5-yl}acetamide
  • Using the procedure described in Example 1 Method A, crude N-[3-amino-4-(ethylamino)phenyl]acetamide is converted to N-{2-{[2-(2,5-Difluorophenyl)-1H-imidazol-1-yl]methyl}-1-ethyl-1H-benzimidazol-5-yl}acetamide.
    • 5. Preparation of 1-Ethyl-2-({[2-(2,5-difluorophenyl)-1H-imidazol-1-yl]methyl}-5-amino-1H-benzimidazole
  • Crude N-{1-Ethyl-2-{[2-(2,5-difluorophenyl)-1H-imidazol-1-yl]methyl}-1H-benzimidazol-5-yl}acetamide is treated with 10% aqueous HCl (20 mL) in methanol (10 mL) at reflux for 1.5 h. After cooling, the methanol is removed in vacuo, and the aqueous layer washed with ethyl acetate. The aqueous layer is adjusted to pH 10 with 3 N NaOH, then extracted with ethyl acetate (3×), and the combined organic extracts washed with water (2×) then saturated aqueous sodium chloride, dried (MgSO4) and concentrated to give 1-Ethyl-2-{[2-(2,5-difluorophenyl)-1H-imidazol-1-yl]methyl}-5-amino-1H-benzimidazole. LCMS MH+ 354.4.
    • Example 10 Synthesis of 1-Ethyl-2-{[2-(3-fluorophenyl)-pyrazol-3-yl]methyl}-5-(1,2,4-triazol-1-yl)-1H-benzimidazole
  • Figure US20100267698A1-20101021-C00041
  • 1-Ethyl-2-{[2-(3-fluorophenyl)-pyrazol-3-yl]methyl}-5-amino-1H-benzimidazole (274 mg) is treated with N,N-dimethylformamide azine hydrochloride (1 eq) in toluene (10 mL) and methoxyethanol (10 mL) and refluxed for 6 h. After cooling and concentrating, the residue is treated with water and extracted with dichloromethane (2×). The aqueous layer is adjusted to pH 8 with aqueous sodium bicarbonate and extracted with dichloromethane (2×). The combined organic extracts are washed with saturated aqueous sodium chloride, dried (MgSO4), concentrated, purified by preparative silica gel chromatography, and triturated with ether to afford 1-Ethyl-2-{[2-(3-fluorophenyl)-pyrazol-3-yl]methyl}-5-(1,2,4-triazol-1-yl)-1H-benzimidazole, which is converted to the hydrochloride salt in ethyl acetate. LCMS MH+ 406.2.
    • Example 11 Synthesis of 1-ethyl-2-{[2-(2,5-difluorophenyl)-1H-imidazol-1-yl]methyl}-5-(1,2,3-triazol-1-yl-4-carboxylate)-1H-benzimidazole
  • Figure US20100267698A1-20101021-C00042
    • 1. Preparation of 1-Ethyl-2-{[2-(2,5-difluorophenyl)-1H-imidazol-1-yl]methyl}-5-azido-1H-benzimidazole
  • To an ice-cold solution of 1-ethyl-2-{[2-(2,5-difluorophenyl)-1H-imidazol-1-yl]methyl}-5-amino-1H-benzimidazole (466 mg) in acetic acid (8 mL) a solution of sodium nitrite (1.1 eq) in water (4 mL) is added dropwise. After stirring at 0° C. for 1 h, a solution of sodium azide (1.3 eq) in water (5 mL) is added dropwise. Stirring is continued for 0.5 h at 0° C., and then at room temperature for 0.5 h. The reaction mixture is concentrated to ⅓ volume, water (10 mL) is added and then mixture is then extracted with ethyl acetate (3×). The combined organic layers are washed with aqueous sodium bicarbonate (2×), then washed with saturated aqueous sodium chloride, dried (MgSO4), and concentrated to give 641 mg of crude 1-Ethyl-2-{[2-(2,5-difluorophenyl)-1H-imidazol-1-yl]methyl}-5-azido-1H-benzimidazole.
    • 2. Preparation of 1-{2-[2-(2,5-Difluoro-phenyl)-imidazol-1-ylmethyl]-1-ethyl-1H-benzoimidazol-5-yl}-1H-[1,2,3]triazole-4-carboxylic acid ethyl ester
  • A mixture of crude 1-ethyl-2-{[2-(2,5-difluorophenyl)-1H-imidazol-1-yl]methyl}-5-azido-1H-benzimidazole (143 mg) and ethyl propiolate (1 eq.) in ethanol (10 mL) is heated at reflux for 6 h. The mixture is cooled, concentrated, and the residue purified by preparative silica gel chromatography to give 1-{2-[2-(2,5-Difluoro-phenyl)-imidazol-1-ylmethyl]-1-ethyl-1H-benzoimidazol-5-yl}-1H-[1,2,3]triazole-4-carboxylic acid ethyl ester. 1H NMR (CDCl3): δ 8.51 s, (1H), 8.05 (s, 1H), 7.74 (m, 1H), 7.50-7.39 (m, 4H), 7.21-7.17 (m, 2H), 7.06 (s, 1H), 5.54 (s, 2H), 4.47 (q, 2H), 3.86 (q, 2H), 1.43 (t, 3H), 1.04 (t, 3H), LCMS MH+ 460.6.
    • Example 12 Synthesis of 1-ethyl-2-{[2-(2,5-difluorophenyl)-pyrazol-3-yl]methyl}-5-(1,2,3,4-tetrazol-1-yl)-1H-benzimidazole
  • Figure US20100267698A1-20101021-C00043
  • 1-Ethyl-2-{[2-(2,5-difluorophenyl)-pyrazol-3-yl]methyl}-5-amino-1H-benzimidazole (104 mg) is treated with triethylorthoformate (4 eq) in acetic acid (10 mL) at reflux for 4 h. After concentrating the solution, acetic acid is re-added, sodium azide (4 eq) added, and the mixture heated at 70° C. for 3 h. After cooling, water (15 mL) is added and the mixture concentrated. The residue is taken up in ethyl acetate, washed with aqueous sodium bicarbonate (2×) then saturated aqueous sodium chloride, dried (MgSO4), concentrated, and purified by preparative silica gel chromatography to give 1-ethyl-2-{[2-(2,5-difluorophenyl)-pyrazol-3-yl]methyl}-5-(1,2,3,4-tetrazol-1-yl)-1H-benzimidazole, which is converted to the hydrochloride salt in ethyl acetate. LCMS MH+ 395.018
    • Example 13 Synthesis of 1-Ethyl-2-{[2-(2,5-difluorophenyl)-pyrazol-3-yl]methyl}-5-(5-methyl-oxazol-2-yl)-1H-benzimidazole
  • Figure US20100267698A1-20101021-C00044
    • 1. Preparation of N-propargyl 1-ethyl-2-{[2-(2,5-difluorophenyl)-pyrazol-3-yl]methyl}-1H-benzimidazole-5-carboxamide
  • Oxalyl chloride (2.5 eq of 2M in dichloromethane) is added dropwise to a solution of 1-ethyl-2-{[2-(2,5-difluorophenyl)-pyrazol-3-yl]methyl}-1H-benzimidazole-5-carboxylate (368 mg) in DMF (5 drops) and dichlormethane (30 mL) at 0° C. The mixture is stirred at 0° C. for 0.5 h, then at room temperature for 1 h. The solution is concentrated, the residue taken up in DMF (30 ml), excess propargylamine added, and the mixture stirred for 6 h. Dilute aqueous sodium bicarbonate is added then extracted with dichloromethane (3×), the combined organic layers washed with saturated aqueous sodium chloride, dried (MgSO4), and concentrated to give 462 my of crude N-propargyl 1-ethyl-2-{[2-(2,5-difluorophenyl)-pyrazol-3-yl]methyl}-1H-benzimidazole-5-carboxamide.
    • 2. Preparation of 1-Ethyl-2-{[2-(2,5-difluorophenyl)-pyrazol-3-yl]methyl}-5-(5-methyl-oxazol-2-yl)-1H-benzimidazole
  • A mixture of crude N-propargyl 1-ethyl-2-{[2-(2,5-difluorophenyl)-pyrazol-3-yl]methyl}-1H-benzimidazole-5-carboxamide (450 mg) and mercury(II) acetate (1 eq) is heated at reflux in acetic acid (15 mL) for 6 h. The mixture is concentrated, saturated aqueous potassium carbonate added and extracted with ethyl acetate (3×). The combined organic layers were washed with water then with saturated aqueous sodium chloride, dried (MgSO4), concentrated, and triturated with ether to give 1-ethyl-2-{[2-(2,5-difluorophenyl)-pyrazol-3-yl]methyl}-5-(5-methyl-oxazol-2-yl)-1H-benzimidazole, which is converted to the hydrochloride salt in ethyl acetate. 1H NMR (d6 DMSO): δ 8.16 (s, 1H), 8.09-9.01 m. (2H), 7.78 (m, 1H), 7.62 (m, 1H), 7.49-7.45 (m, 3H), 7.04 (m, 1H), 6.56 (s, 1H), 4.70 (s, 2H), 4.36 (q, 2H), 2.41 (s, 3H), 1.21 (t, 3H). LCMS MH+ 420.5.
    • Example 14 Synthesis of 2-{[2-(3-Fluorophenyl)-1H-imidazol-1-yl]methyl}-1-ethyl-5-(1,2,4-oxadiazol-3-yl)-1H-benzimidazole
  • Figure US20100267698A1-20101021-C00045
    • 1. Preparation of 1-Ethyl-2-{[2-(2,5-difluorophenyl)-1H-imidazol-1-yl]methyl-1H-benzimidazol}-5-carboxamide oxime
  • 1-Ethyl-2-{[2-(3-fluorophenyl)-1H-imidazol-1-yl]methyl}-5-cyano-1H-benzimidazole (340 mg) is added to a solution of hydroxylamine hydrochloride (137 mg, 2 eq) and triethylamine (0.3 mL, 2.2 eq) in methanol (3 mL), then heated at reflux for 2.5 h. The reaction is cooled, concentrated, water added, the solid collected and rinsed well with water and dried to give 305 mg of 1-Ethyl-2-{[2-(2,5-difluorophenyl)-1H-imidazol-1-yl]methyl-1H-benzimidazol}-5-carboxamide oxime.
    • 2. Preparation of 1-Ethyl-2-{[2-(2,5-difluorophenyl)-1H-imidazol-1-yl]methyl-1H-benzimidazol}-5-carboxamide oxime
  • 1-Ethyl-2-{[2-(3-fluorophenyl)-1H-imidazol-1-yl]methyl-1H-benzimidazol}-5-carboxamide oxime (161 mg) is treated with triethylorthoformate (3 mL) and boron trifluoride-THF complex (0.1 mL) at 100° C. for 2.5 h. 1N HCl (0.5 mL) is added to the hot mixture; the reaction is then cooled and concentrated. After cooling in an ice bath, aqueous sodium hydroxide is added. The solid is collected, rinsed with water, then ether and dried to give 1-Ethyl-2-{[2-(2,5-difluorophenyl)-1H-imidazol-1-yl]methyl-1H-benzimidazol}-5-carboxamide oxime, which is converted to the methanesulfonate salt in acetone. Mp 183-186° C.
    • Example 15 Synthesis of 4-amino-3-ethylaminobenzonitrile
  • Figure US20100267698A1-20101021-C00046
    • 1. 4-nitro-3-chlorobenzonitrile
  • To an ice cold solution of 4-amino-3-chlorobenzonitrile (1 g, 6.6 mmol) in concentrated HCl (2.5 mL) plus water (2.5 mL) a chilled solution of sodium nitrite (0.74 g, 1.62 eq) in water (3.6 mL) is added dropwise to maintain the reaction temperature <0° C. After stirring at 0° C. for 10 min, the mixture is added portion-wise to an ice cold solution of sodium nitrite (3.29 g, 7.22 eq) and copper(I) oxide (349 mg, 0.37 eq) in water 14.5 mL). Stirring is continued at 0° C. for 40 min, and then at room temperature for 0.5 h. The reaction mixture is extracted with dichloromethane (2×), the combined organic layers washed with saturated aqueous sodium chloride, dried (MgSO4), concentrated, and purified by silica gel chromatography to give 4-nitro-3-chlorobenzonitrile. 1H NMR (CDCl3): δ 7.96 (d, J=8.24 Hz, 1 h), 7.88 (d, T=1.65 Hz, 1H), 7.63 (dd, J=1.65, 8.24 Hz, 1H.
    • 2. Preparation of 4-Ethylamino-3-chloro benzonitrile
  • A mixture of 4-nitro-3-chlorobenzonitrile (0.74 g, 4.1 mmol), potassium carbonate (1.68 g, 12.2 mmol), and ethylamine (4 mL, 2M in THF) in DMF (2 mL) is stirred at ambient temperature for 3 h. Additional ethylamine (2.1 mL, 2M in THF) is added, the flask stoppered, and stirring continued an additional 15.5 h. Water (50 mL) is added and extracted 2× with ethyl acetate (50 mL), and the combined organic layers are washed with water, then brine, dried (MgSO4), and concentrated to give a mixture of 3-Ethylamine-4-nitrobenzonitrile and 4-Ethylamino-3-chloro benzonitrile which was carried forward without purification.
    • 3. Preparation of 4-Amino-3-ethylaminobenzonitrile
  • To a suspension of crude 4-ethylamino-3-chloro benzonitrile (above) in conc. HCl (4 mL) tin(II) chloride dihydrate (3.16 g) is added. After stirring at ambient temperature for 1.5 h, the mixture is poured onto ice and the solution mad alkaline with 10 N aq. NaOH. The aqueous solution is extracted 2× with dichloromethane, and the combined organic layers washed with brine, dried (MgSO4), and concentrated. Trituration with ether affords 4-Amino-3-ethylaminobenzonitrile as a white solid. 1H NMR (CDCl3): δ 7.00 (dd, J=1.65, 7.97 Hz, 1H), 6.83 (d, J=1.65 Hz, 1H), 6.67 (d, J=7.97 Hz, 1 h), 3.13 (q, J=7.14 Hz, 1.31 (t, J=7.14 Hz, 3H).
    • Example 16 Synthesis of 2-Fluoro-6-(1H-imidazol-2-yl)-pyridine
  • Figure US20100267698A1-20101021-C00047
    • 1. Preparation of 2-Fluoropyridine-6-carboxaldehyde Method A
  • 2-Fluoro-6-methylpyridine (14.4 g, 0.13 mol) and tert-butoxybis(dimethylamino)methane (Bredereck's reagent; 34.9 g, 0.20 mol) are heated at 140° C. for 24 h. The reaction is cooled and diluted with THF (100 mL). Sodium periodate (75 g) in water (400 mL) is added at 0-5° C., and the reaction mixture is then stirred for 24 h at room temperature. The precipitate is filtered through celite, and the filtrate extracted 5× with diethyl ether. The combined ether layers are washed with water, brine, and dried (MgSO4). Most of the solvent is removed by concentration at 0° C. (by keeping ice in the bath) to provide 2-Fluoropyridine-6-carboxaldehyde.
    • Method B
  • 2-Fluoropyridine-6-carboxaldehyde can also be prepared as follows: To a solution of diisopropylamine (6.54 mL, 1.2 equiv) in 30 mL of THF at 0° C. a solution of n-butyllithium (17.1 mL, 2.5M in hexanes) is added dropwise. Stirring is continued for 15 minutes at 0° C., the reaction is then cooled to −78° C. 2-Fluoro-6-methylpyridine (4.00 mL, 38.9 mmol) is added dropwise to the cold solution. The reaction mixture is stirred at −78° C. for 1 h and then quenched with DMF (4.52 mL, 1.5 equiv). The reaction is maintained at −78° C. for 30 minutes and then warmed to 0° C. The cold solution is added to a mixture of sodium periodate (24.9 g) in 120 mL of water at 0° C. The reaction mixture is allowed to gradually warm to room temperature over 1 h and then stirred at room temperature for 24 h. The reaction mixture is filtered through a plug of celite to remove the precipitate and the plug is washed with ether. The organic layer is separated, washed with aqueous sodium bicarbonate (1×40 mL), then with 0.25M KH2PO4 (1×40 mL) and then brine (1×40 mL). The organic solution is dried (NaSO4) and concentrated in vacuo.
    • 2. Preparation of 2-Fluoro-6-(1H-imidazol-2-yl)-pyridine
  • To a solution of the crude aldehyde from step 1, Method B (above) in methanol (12 mL) aqueous glyoxal (6.21 mL, 40 wt. % in water) is added dropwise. The solution is cooled to 0° C. and aqueous ammonium hydroxide (6.0 mL, 28 wt. % in water) is added. The reaction is allowed to warm to room temperature gradually over about an hour and then stirred another 3 h at room temperature. Most of the methanol is removed in vacuo, the reaction mixture diluted with water (10 mL) and extracted with ethyl acetate (30 mL). The organic layer is washed with brine (20 mL), diluted with hexanes (15 mL), passed through a plug of silica gel (¼ inch deep×1¼ inch diameter), and the plug washed with more 2:1 ethyl acetate/hexanes (20 mL). The combined eluents are concentrated in vacuo to yield crude 2-Fluoro-6-(1H-imidazol-2-yl)-pyridine.
    • Example 17 Synthesis of 2-Fluoro-6-(1H-imidazol-2-yl)-pyridine acetic acid 1. Preparation of [2-(6-fluoropyridin-2-yl)-imidazol-1-yl]acetic acid tert-butyl ester
  • A mixture of 2-Fluoro-6-(1H-imidazol-2-yl)pyridine (410 mg; 2.51 mmol), tert-butyl bromoacetate (539 mg; 2.76 mmol), potassium carbonate (520 mg; 3.77 mmol), and N,N-dimethylformamide (20 mL) is stirred at room temperature for 16 h. Water (60 mL) is added and the mixture extracted with ethyl acetate (3×70 mL). The organic extracts are washed with water (3×40 mL) and brine (1×40 mL), dried (MgSO4), and concentrated to give [2-(6-Fluoropyridin-2-yl)-imidazol-1-yl]acetic acid tert-butyl ester as an orange oil (630 mg). 1H NMR (CDCl3) δ 8.12 (dd, 1H), 7.82 (q, 1H), 7.16 (s, 1H), 6.98 (s, 1H), 6.81 (dd, 1H), 5.14 (s, 2H), 1.44 s, (9H), LCMS: 276.1 (M−).
    • 2. Preparation of 2-Fluoro-6-(1H-imidazol-2-yl)-pyridine acetic acid
  • A solution of [2-(6-fluoropyridin-2-yl)-imidazol-1-yl]acetic acid tert-butyl ester (630 mg; 2.27 mmol) and trifluoroacetic acid (4 mL) in dichloromethane (4 mL) is stirred at room temperature for 5 hr. The reaction mixture is concentrated. The residue is treated with 3M HCl in ethyl acetate (10 mL) and the suspension stirred at room temperature for 1 hr. The precipitate is collected, washed with ethyl acetate, and dried to give 2-Fluoro-6-(1H-imidazol-2-yl)-pyridine acetic acid as a tan solid (581 mg), 1H NMR (d6DMSO) δ 8.35-8.26 (m, 2H) 7.83 (s, 1H), 7.76 (s, 1H), 7.44-7.41 (m. 1H) 5.40 (s, 2H). LCMS: 222.1 (MH+).
    • Example 18 Synthesis of 2-{[2-(2,5-di-fluorophenyl)-1H-imidazol-1-yl]methyl}-ethyl-1H-imidazo[4,5-b]pyridine
  • Figure US20100267698A1-20101021-C00048
    • 1. Preparation of 3-ethylamino-2-nitro-pyridine
  • Ice cold ethylamine (0.55 mL) was added to a solution of 3-fluoro-2-nitropyridine (400 mg, 2.8 mmol; prepared according to N. Plé and G. Queguiner, J. Heterocyclic Chem, 1989, 26, 475-476) in 2:1 DMF:THF (6 mL) at ambient temperature, and the mixture stirred for 0.75 h. Water was added, then extracted 2× with ethyl acetate. The combined organic layers were washed with water then with saturated aqueous sodium chloride, dried (MgSO4), and concentrated to give 3-ethylamino-2-nitro-pyridine as a gold solid. 1H NMR (CDCl3): δ 7.87 (d, 1 h0, 7.43 (dd, 1H), 7.35 (d, 1H), 3.38 (pentet, 2H), 1.4 (t, 3H). LCMS: 121.1 (MH+—NO2).
    • 2. Preparation of 2-Amino-3-ethylaminopyridine
  • A mixture of 3-ethylamino-2-nitro-pyridine (400 mg), 10% palladium on carbon (40 mg) and 1:1 ethyl acetate:methanol (30 mL) were placed in a Parr apparatus under hydrogen (50 psi) for 50 min. The mixture was filtered through Celite, concentrated, and the product triturated with hexane to give 2-Amino-3-ethylaminopyridine as a brown solid. More material was recovered from the filtrate. 1H NMR (CDCl3): δ 7.59 (d, J=4.94 Hz, 1H), 6.81 (d, J=6.32 Hz, 1H), 6.71 (dd, J=4.94, 7.69 Hz, 1H), 3.12 (q, J=7.14 Hz, 2 h), 1.31 (t, J=7.14 Hz, 3H).
    • 3. Preparation of 2-{[2-(2,5-Difluorophenyl)-1H-imidazol-1-yl]methyl}-1-ethyl-1H-imidazo[4,5-b]pyridine
  • 2-Amino-3-ethylaminopyridine (60 mg, 0.43 mmol) and methyl 1-carboxymethyl-2-(2,5-difluorophenyl)-imidazole (193 mg, 0.76 mmol) were used according to Example 1 Method B to prepare 2-{[2-(2,5-Difluorophenyl)-1H-imidazol-1-yl]methyl}-1-ethyl-1H-imidazo[4,5-b]pyridine, which is converted to the hydrochloride salt in ethyl acetate. m.p. 214-216° C. LCMS MH+ 322.2, MH− 320.3.
    • Example 19 Synthesis of 2-{[2-(3-Fluorophenyl)-1H-imidazol-1-yl]methyl}-1-ethyl-3H-imidazo[4,5-c]pyridine
  • Figure US20100267698A1-20101021-C00049
    • 1. Preparation of 3-Chloro-4-nitropyridine N-oxide
  • Aqueous hydrogen peroxide (30 mL of 30%) is added dropwise to an ice cold mixture of 3-chloropyridine (6.0 g, 53 mmol) in acetic anhydride (30 mL). The mixture is allowed to stir at ambient temperature for 24 h, water is then added and the mixture concentrated. The residue is taken up in concentrated sulfuric acid (10 mL) and fuming sulfuric acid (5 mL), and then cooled to 0° C. Concentrated nitric acid (24 mL) is added slowly, and the ice bath removed. The reaction is then heated at reflux for 2 h, cooled, and poured into ice water. Ammonium bicarbonate is added carefully until pH 8 is achieved, the solution is then extracted with dichloromethane. The organic layer is washed with water, dried (NaSO4), and concentrated to give 3-Chloro-4-nitropyridine N-oxide as a yellow solid. 1H NMR (CDCl3). δ 8.47 (d, J=4 Hz, H), 8.35 (dd, J=5, 12 Hz, 1H), 7.73 (ddd, J=4, 9, 12 Hz, 1H).
    • 2. Preparation of 3-Ethylamino-4-nitro-3-pyridine N-oxide
  • To an ice cold solution of 3-chloro-4-nitropyridine N-oxide (2.93 g, 16.8 mmol) in ethanol (30 mL) ethylamine (25 mL of 2M in THF) is added. The mixture is allowed to stir overnight at ambient temperature, then concentrated in vacuo to afford crude 3-Ethylamino-4-nitro-3-pyridine N-oxide as a yellow oil. 1H NMR (CDCl3): δ 8.01 (d, J=7 Hz, H), 7.91 (s, 1H), 7.45 (d, J=7 Hz, 1H), 3.31 (q, J=7 Hz, 2H), 1.40 (t, J=7 Hz, 3H).
    • 3. Preparation of 3-Ethylamino-4-aminopyridine
  • Crude 3-ethylamino-4-nitro-3-pyridine N-oxide and 10% palladium on carbon (1 g) in methanol (30 mL) are placed in a Parr apparatus under hydrogen (60 psi) for 2 days. Additional 10% palladium on carbon (880 mg) is added and returned under hydrogen (60 psi) for 2 days. The mixture was filtered through Celite, concentrated, and the product triturated with hexane-ether to afford 1.3 g of 3-Ethylamino-4-aminopyridine. 1H NMR (d6-DMSO): δ 7.63 (d, J=6 Hz, 1H), 7.47 (s, 1H), 6.57 (d, J=6 Hz, 1H), 3.05 (q, J=7 Hz, 2H), 1.21 (t, J=7 Hz, 3H).
    • 4. Preparation of 2-{[2-(3-Fluorophenyl)-1H-imidazol-1-yl]methyl}-1-ethyl-3H-imidazo[4,5-c]pyridine
  • 3-Ethylamino-4-aminopyridine (548 mg, 3.99 mmol) and methyl 1-carboxymethyl-2-(3-fluorophenyl)-imidazole (467 mg, 1.99 mmol) were used according to Example 1 Method B to prepare 2-{[2-(3-Fluorophenyl)-1H-imidazol-1-yl]methyl}-1-ethyl-3H-imidazo[4,5-c]pyridine. 1H NMR (CDCl3): δ 1.08 (t, J=7 Hz, 3H), 3.89 (q, J=7 Hz, 2H), 5.52 (s, 2H), 7.04 (s, 1H), 7.16-7.20 (m, 2H), 7.37-7.50 (m, 3H), 7.68 (d, J=6 Hz, 1H), 8.47 (d, J=6 Hz, 1H), 8.78 (s, 1H).
    • Example 20 Synthesis of 3-fluoro-(1H-imidazol-2-yl)benzene
  • Figure US20100267698A1-20101021-C00050
  • Saturated ammonium hydroxide solution (30 mL) is slowly added to a solution of 3-fluorobenzaldehyde (12.4 g, 100 mmol) and glyoxal (17.5 mL of 40% wt in water, 120 mmol) in methanol (100 mL) at ambient temperature. After stirring for 24 h, most of the solvent is removed at reduced pressure. Benzene is added and evaporated to remove residual water. The resulting dark oil is purified by chromatography on silica gel (2% MeOH/CH2Cl2) to obtain a tan solid. Trituration with ether/hexane provides 3-fluoro-(1H-imidazol-2-yl)benzene as a white solid. LRMS m/z (M+1) 163.2.
    • Example 21 Synthesis of 3-chloro-4-fluoro-(1H-imidazol-2-yl)benzene
  • Figure US20100267698A1-20101021-C00051
  • A mixture of 3-chloro-4-fluoro-benzaldehyde (0.032 mol), glyoxal (40% in water, 0.038 mol) and ammonium hydroxide (28% in water, 0.16 mol) in MeOH (60 mL) is stirred at room temperature overnight. Solvent is removed in vacuo and the residue is partitioned between water and CH2Cl2. The organic layer is washed with brine, dried (Na2SO4), and concentrated. The residue is purified by column chromatography on silica gel eluting with CH2Cl2/MeOH (95/5) to afford 3-chloro-4-fluoro-(1H-imidazol-2-yl)benzene as a yellow solid. 1H NMR (CDCl3) δ 7.88 (dd, 1H), 7.70 (m, 1H), 7.19 (t, 1H), 7.17 (s, 2H). LRMS m/z (M+1) 197.0.
    • Example 22 Synthesis of 2,3,4-trifluoro-(1H-imidazol-2-yl)benzene
  • Figure US20100267698A1-20101021-C00052
  • Saturated ammonium hydroxide solution (26 mL) is slowly added to a solution of 2,3,4-trifluorobenzaldehyde (5.0 g, 31.2 mmol) and glyoxal (10.75 mL of 40% wt in water, 93.7 mmol) in methanol (100 mL) at ambient temperature. After stirring for 24 h, most of the solvent is removed at reduced pressure. Benzene is added and evaporated to remove residual water. The resulting dark oil is purified by chromatography on silica gel (5% MeOH/CH2Cl2) to obtain a tan solid. Trituration with ether/hexane provides 2,3,4-trifluoro-(1H-imidazol-2-yl)benzene as a white solid. LRMS m/z (M+1) 199.10.
    • Example 23 Synthesis of 2-(1H-imidazol-2-yl)-thiazole
  • Figure US20100267698A1-20101021-C00053
    • 1. Preparation of 1-ethoxymethyl-2-tributylstannanyl-1H-imidazole
  • Figure US20100267698A1-20101021-C00054
  • 1.6 M n-BuLi (12.0 mL, 19.2 mmol) is slowly added to a solution of 1-ethoxymethyl-1H-imidazole [available via the procedure outlined in Tang, C. C.; Davalian, D.; Huang, P.; Breslow, R. J. Am. Chem. Soc. 1978, 100, 3918] (2.20 g, 17.4 mmol) in THF (30 mL) at −78° C. under N2. The reaction mixture is stirred at −78° C. for 20 min. whereupon tributyltin chloride (5.7 mL, 20.9 mmol) is slowly added. The reaction mixture is stirred at −78° C. for 10 min. and then warmed to room temperature. After stirring at room temperature for 1.5 h, the reaction mixture is concentrated in vacuo. The residue is triturated with hexanes and filtered, and the filtrate is concentrated in vacuo. The residue is again triturated with hexanes and filtered, and the filtrate concentrated in vacuo. The 1H NMR of the resulting oil indicates a 2:1 mixture of 1-ethoxymethyl-2-tributylstannanyl-1H-imidazole: 1-ethoxymethyl-1H-imidazole. This material is used in the next reaction without further purification. Selected 1H NMR resonances (400 MHz, CDCl3) δ 7.21 (s, 1H), 7.14 (s, 1H), 5.24 (s, 2H) ppm.
    • 2. Preparation of 2-(1-ethoxymethyl-1H-imidazol-2-yl)-thiazole
  • Figure US20100267698A1-20101021-C00055
  • A solution of crude 1-ethoxymethyl-2-tributylstannanyl-1H-imidazole (previous experimental), 2-bromothiazole (1.05 mL, 11.6 mmol, 1.0 eq based on integration of 1H NMR of crude 1-ethoxymethyl-2-tributylstannanyl-1H-imidazole), and Pd(PPh3)4 (0.67 g, 0.58 mmol) in toluene (20 mL) is stirred at 80° C. for 18 h. After cooling to room temperature, the reaction mixture is poured into saturated aqueous NaHCO3 and extracted twice with CH2Cl2. The combined extracts are dried over Na2SO4 and concentrated in vacuo. The residue is purified by flash chromatography on silica gel, eluting with 2:1 hexanes-EtOAc (+0.5% Et3N). Fractions containing product are concentrated and resubjected to flash chromatography on silica gel. Elution with 2:1 hexanes-EtOAc (+0.5% Et3N) affords (26%) of 2-(1-ethoxymethyl-1H-imidazol-2-yl)-thiazole as a bright yellow oil. 1H NMR (400 MHz, CDCl3) δ 7.82 (d, J=3.2 Hz, 1H), 7.33 (d, J=3.2 Hz, 1H), 7.20 (d, J=1.2 Hz, 1H), 7.15 (d, J=1.2 Hz, 1H), 6.03 (s, 2H), 3.56 (q, J=7.2 Hz, 2H), 1.17 (t, J=7.2 Hz, 3H) ppm.
    • 3. Preparation of 2-(1H-imidazol-2-yl)-thiazole
  • Figure US20100267698A1-20101021-C00056
  • Concentrated HCl (10 ml) is added to a solution of 2-(1-ethoxymethyl-1H-imidazol-2-yl)-thiazole (940 mg, 4.49 mmol) in 24 mL of 1:1 EtOH—H2O at room temperature. The solution is stirred at reflux for 3 h. The reaction mixture is then cooled to 0° C. and made basic by the addition of about 12 mL of 10 N aqueous NaOH. The mixture is back titrated to approximately pH 4 using concentrated HCl. Solid NaHCO3 is added to the point of saturation and approximately pH 8. The mixture is then extracted twice using a mixture of THF and EtOAc. The combined extracts are dried over Na2SO4 and concentrated to an oily solid, which is triturated with a small amount of CH2Cl2. The solid is collected by filtration. The filtrate is concentrated, and the oily solid triturated once more with CH2Cl2. The second resultant solid is collected by filtration and combined with the solid first obtained. The product, 2-(1H-imidazol-2-yl)-thiazole, is obtained as a slightly off-white solid. 1H NMR (400 MHz, DMSO-d6) δ 13.04 (br, 1H), 7.87 (d, J=3.2 Hz, 1H), 7.70 (d, J=3.2 Hz, 1H), 7.14 (br, 2H) ppm.
  • The compound of this example can be prepared essentially according to the procedures described in Example 21.
    • Example 24 Synthesis of 2-(1H-Imidazol-2-yl)-pyrimidine hydrochloride
  • Figure US20100267698A1-20101021-C00057
  • A mixture of 2-Cyanopyrimidine (8.0 g, 76 mmol, prepared according to Liebigs Ann. Chem. 2, 1981, 333-341) and Aminoacetaldehyde dimethyl acetal (8 g, 76 mmol) is heated at 100° C. for 4 hours, cooled, and 100 mL of MeOH and 5 mL of concentrated HCl are added. The mixture is heated at reflux with stirring for 30 hours, cooled and evaporated to dryness in vacuo. 50 mL of i-ProH is added to the residue and the mixture is heated at reflux with stirring for 30 minutes and cooled. The crystals are collected by filtration, washed with ether, and dried to give the title compound. 1H NMR (DMSO): δ 9.08 (d, 2H), 7.87 (s, 2H), 7.75 (t, 1H). LRMS calcd 146. found 147 (MH+).
    • Example 25 Synthesis of 2-(1H-Imidazol-2-yl)-thiazole-4-carbonitrile
  • Figure US20100267698A1-20101021-C00058
    • 1) Preparation of 1-Dimethylsulfamoyl-1H-imidazole-2-carbothioic acid methoxymethyl-amide
  • To a solution of 1-Dimethylsulfamoyl-1-imidazole (2.0 g, 11.4 mmol) in 10 mL of anhydrous THF at −78° C. is added a solution of n-BuLi in Hexane (2.5 M, 4.6 mL) dropwise under N2. After the mixture is stirred at the same temperature for about one hour, Methoxymethyl isocyanate (1.2 g, 11.4 mmol) is added dropwise at −78° C. The reaction mixture is stirred at −78° C. for about 6 hours and slowly warmed to room temperature. After quenched with a saturated aqueous NH4Cl solution (20 mL), the mixture is extracted with Ethyl acetate (20 mL×2). The extract is washed with water, dried and concentrated. The residue is purified on a silica gel column with 3% MeOH in CH2Cl2 as eluent to give the title compound. 1H NMR (CDCl3): δ 8.60 (br, 1H), 7.32 (d, 1H), 7.01 (d, 1H), 5.22 (d, 2H), 3.51 (s, 3H), 3.00 (d, 6H). LRMS calcd 278. found 279 (MH+).
    • 2) Preparation of 1H-Imidazole-2-carbothioic acid amide
  • A solution of 1-Dimethylsulfamoyl-1H-imidazole-2-carbothioic acid methoxymethyl-amide (1.0 g, 3.6 mmol) in Acetic acid (10 mL) and water (2 mL) is stirred at 50° C. overnight. After the volatiles are evaporated in vacuo, the residue is partitioned between Ethyl acetate (40 mL) and aqueous NaHCO3 (10 mL). The organic layer is separated, washed with water, dried and concentrated in vacuo to a solid (250 mg). 1H NMR (DMSO): δ 12.7 (s, 1H), 9.65 (s, 1H), 9.42 (s, 1H), 7.24 (s, 1H), 7.15 (s, 1H.
    • 3) Preparation of 2-(1H-Imidazole-2-yl)-thiazole-4-carboxylic acid ethyl ester
  • A mixture of 1H-Imidazole-2-carbothioic acid amide (200 mg, 1.57 mmol) and Ethyl bromopyruvate (340 mg, 1.57 mmol) is heated at 75° C. for about 5 hours. After the volatiles are evaporated in vacuo, the residue is partitioned between Ethyl acetate (40 mL) and aqueous NaHCO3 (10 mL). The organic layer is separated, washed with water, dried and concentrated. The residue is purified on a silica gel column with 3% MeOH in CH2Cl2 as eluent to give 200 mg of the titled compound. 1H NMR (CDCl3): δ 8.15 (s, 1H), 7.20 (br, 1H), 7.10 (br, 1H), 4.33 (q, 2H), 1.23 (t, 3H). LRMS calcd 223. found 224 (MH+).
    • 4) Preparation of 2-(1H-Imidazole-2-yl)-thiazole-4-carboxylic acid amide
  • A solution of 2-(1H-Imidazole-2-yl)-thiazole-4-carboxylic acid ethyl ester (190 mg) in 5 mL of MeOH is saturated with NH3 gas, and heated at 60° C. in a sealed tube overnight. After cooled, the reaction mixture is transferred to a round bottle flask and evaporated in vacuo to a solid. LRMS calcd 194. found 195 (MH+).
    • 5) Preparation of 2-(H-Imidazole-2-yl)-thiazole-4-carbonitrile
  • To a solution of 2-(1H-Imidazole-2-yl)-thiazole-4-carboxylic acid amide (120 mg) in 2 mL of anhydrous pyridine is added 0.25 mL of POCl3 dropwise at 0° C. The reaction mixture is stirred at the same temperature for 2 hours, poured into ice water, neutralized with solid NaHCO3, and extracted with 5% MeOH in CH2Cl2. The extract is washed with brine, dried and concentrated to a solid. 1H NMR (DMSO): δ 13.45 (br, 1H), 8.82 (s, 1H), 7.38 (s, 1H), 7.12 (s, 1H). LRMS calcd 176. found 177 (MH+).
    • Example 26 Synthesis of 2-[2-(6-fluoro-pyridin-2-yl)-imidazol-1-ylmethyl]-1-propyl-1-H-imidazo[4,5-c]pyridine
  • Figure US20100267698A1-20101021-C00059
    • 1. Preparation of (3-nitro-pyridin-4-yl)-propyl-amine
  • To a solution of 4-chloro-3-nitro-pyridine (18.0 g, 114 mmol) in EtOH(300 ml) is added n-propylamine (46.9 ml, 568 mmol) slowly at 0° C. and the resulting yellow mixture is warmed to room temp. After stirring for 2 h, the reaction is concentrated in vacuo. The residue is purified by flash chromatography (eluent 50% Hex/EtOAc) to give (3-nitro-pyridin-4-yl)-propyl-amine as a yellow solid. 1H NMR (CDCl3) δ 9.16 (s, 1H), 8.25 (d, J=6 Hz, 1H), 8.15 (br s, 1H), 6.68 (d, J=6 Hz, 1H), 3.29 (q, J=6.8 Hz, 2H), 1.74 (sextet, J=7.2 Hz, 2H), 1.02 (t, J=7.6 Hz, 3H). LRMS calcd 181.19. found 182.5 (MH+).
    • 2. Preparation of N-propyl-pyridine-3,4-diamine
  • A solution of (3-nitro-pyridin-4-yl)-propyl-amine (19.7 g, 109 mmol) in abs. MeOH(150 ml) is hydrogenated at room temp. and 50 psi in the presence of 10% palladium on carbon (1.9 g) for 15 h. Removal of catalyst, concentration of filtrate, and recrystallization the residue in dichloromethae-hexane yields N-propyl-pyridine-3,4-diamine as a tan solid. 1H NMR (DMSO-d6) δ 7.59 (s, 1H), 7.55 (d, J=5.4 Hz, 1H), 6.30 (d, J=5.4 Hz, 1H), 5.26 (br s, 1H), 4.55 (br s, 2H), 3.02 (q, J=6.6 Hz, 2H), 1.56 (sextet, J=7.1 Hz, 2H), 0.92 (t, 7.2 Hz, 3H). LRMS calcd 151.21. found 152.4 (MH+).
    • 3. Preparation of 2-[2-(6-fluoro-pyridin-2-yl)-imidazol-1-ylmethyl]-1-propyl-1-H-imidazo[4,5-c]pyridine
  • Trimethylaluminum (11 ml of 2.0M in toluene, 22.4 mmol) is added dropwise to a solution of N-propyl-pyridine-3,4-diamine (1.70 g, 11.2 mmol) in dichloromethane (50 ml), and the mixture is stirred for 30 minutes at room temperature. To this is added a solution of [2-(6-fluoro-pyridin-2-yl)-imidazol-1-yl]-acetic acid methyl ester (1.32 g, 5.61 mmol) in dichloromethane (10 ml) The mixture is heated at reflux for 18 hours. The resulting brown mixture is poured into ice-water and filtered through celite. The filtrate is extracted with dichloromethane and the combined extracts are washed with saturated brine, dried over anhydrous Na2SO4, filtered, concentrated in vacuo, and chromatographed on silica gel to afford 2-[2-(6-fluoro-pyridin-2-yl)-imidazol-1-ylmethyl]-1-propyl-1-H-imidazo[4,5-c]pyridine as a pale yellow solid. 1H NMR (CDCl3) δ 9.06 (s, 1H), 8.42 (d, J=5.6 Hz, 1H), 8.18 (dd, J=2.1, 7.6 Hz, 1H), 7.88 (dd, J=7.6, 8.4 Hz, 1H), 7.29 (d, J=5.6 Hz, 1H), 7.22 (s, 1H), 7.18 (s, 1H), 6.88 (dd, J=2.4, 8.4 Hz, 1H), 6.29 (s, 2H), 4.26 (t, J=7.6 Hz, 2H), 1.68 (sextet, J=7.2 Hz, 2H), 0.84 (t, J=7.2 Hz, 3H). LRMS calcd 336.37. found 337.6 (MH+).
    • Example 27 Synthesis of 3-Ethyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-3H-imidazo[4,5-c]pyridine
  • Figure US20100267698A1-20101021-C00060
    • 1. Preparation of 3-Chloro-4-nitro-pyridine-1-oxide
  • Aqueous 30% H2O2 (60 mL) is added dropwise to a magnetically stirred solution of 3-chloro-pyridine (12 g, 105 mmol) in acetic anhydride (60 mL) under cold conditions (0 to 10° C.). The resulting mixture is allowed to warm up to room temperature slowly and then stirred overnight at room temperature. The reaction mixture is quenched with water (50 mL), diluted with toluene and concentrated to obtain the crude N-oxide as an oil in near quantitative yield.
  • Fuming H2SO4 (25 mL) is added dropwise to a solution of crude 3-chloro-pyridine-1-oxide in concentrated H2SO4 (25 mL) under cold conditions (0° C.) with stirring. HNO3 (fuming, 90%, 60 mL) is added carefully to the above mixture with caution to keep the offset of any exotherm under control, and then allowed to warm to room temperature slowly. The resulting mixture is then heated at 120° C. for 4 h with stirring, cooled, poured into ice-cold water, and extracted with CHCl3. The combined organic phase is washed successively with saturated aqueous NaHCO3, water, brine, dried over Na2SO4, and concentrated in vacuo to afford 3-Chloro-4-nitro-pyridine-1-oxide as an yellow solid. 1H NMR (300 MHz, CDCl3): δ 8.31 (d, J=1.5 Hz, 1H), 8.13 (dd, J=1.5, 5.4 Hz, 1H), 7.95 (d, J=7.2 Hz, 1H).
    • 2. Preparation of 3-Ethylamino-4-nitro-pyridine-1-oxide
  • Anhydrous K2CO3 (19 g, 144.5 mmol) is suspended in a solution of 3-Chloro-4-nitro-pyridine-1-oxide (10 g, 57.8 mmol) in anhydrous acetonitrle (100 mL). Excess diethyl amine (2.0 M) in THF is added to the above suspension under cold conditions (ice-bath). After complete addition of diethyl amine, the reaction mixture is allowed to warm to room temperature and left stirring overnight. The reaction mixture is filtered to remove K2CO3 and the filtrate evaporated under reduced pressure to remove volatile solvents. The organic residue is subjected to chromatography, eluting with 30% EtOAc-hexanes to afford 3-Ethylamino-4-nitro-pyridine-1-oxide as an orange solid. 1H NMR (300 MHz, CDCl3): δ8.0 1 (d, J=7.5 Hz, 1H), 7.91 (s, 1H), 7.8 (brs, NH), 7.44 (d, J=7.2 Hz, 1H), 3.30 (t, J=7.2 Hz, 2H), 1.39 (t, J=7.2 Hz, 3H), m/z 184 [M+1]
    • 3. Preparation of N3-Ethyl-pyridine-3,4-diamine
  • A solution of 3-ethylamino-4-nitro-pyridine 1-oxide (1.5 g, 8.19 mmol), in methanol (30 mL) is hydrogenated over 10% Pd—C (1.5 g) at 50-60 psi for 48 h. The catalyst is removed by filtration through a pad of celite, and the solvent is evaporated under vacuum to afford N3-Ethyl-pyridine-3,4-diamine as an white solid. 1H NMR (300 MHz, CDCl3): δ 7.59 (s, 1H), 7.57 (d, J=2.4 Hz, 1H), 6.56 (d, J=5.4 Hz, 1H), 3.20 (t, J=7.2 Hz, 2H), 1.29 (t, J=7.2 Hz, 3H), m/z 138 [M+1]
    • 4. Preparation of N-(3-Ethylamino-pyridin-4-yl)-2-(2-thiazol-2-yl-imidazol-1-yl)-acetamide
  • A solution (2.0 M) of Me3Al (4.8 mL, 9.6 mmol) in toluene is added to a solution of the N3-ethyl-pyridine-3,4-diamine (1.06 g, 7.70 mmol) in anhydrous dichloromethane (40 mL) slowly under nitrogen, and the resulting mixture is stirred at room temperature for 1 h. (2-Thiazol-2-yl-imidazol-1-yl)-acetic acid methyl ester (860 mg, 3.85 mmol) is added to the above reaction mixture. The resulting suspension is refluxed overnight and then cooled to room temperature. The reaction mixture is decomposed with a few drops of MeOH. The resulting brown precipitate is suspended in 15% MeOH in CH2Cl2 (200 mL), subjected to sonication for 25 min, filtered through a pad of celite, and concentrated to afford a mixture of 3-Ethyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-3H-imidazo[4,5-c]pyridine and N-(3-ethylamino-pyridin-4-yl)-2-(2-thiazol-2-yl-imidazol-1-yl)-acetamide in a 2:1 ratio which is carried over to the next step without further purification. A portion of the crude residue chromatographed over silica gel eluting with 7% MeOH—CH2Cl2 containing few drops of NH4OH to obtain an analytical sample of N-(3-ethylamino-pyridin-4-yl)-2-(2-thiazol-2-yl-imidazol-1-yl)-acetamide for characterization purposes. 1H NMR (300 MHz, CD3OD): δ 7.95 (s, 1H) 7.87 (d, J=3.3 Hz, 1H), 7.79 (d, J=5.4 Hz, 1H), 7.61 (d, J=3.3 Hz, 1H), 7.42 (d, J=5.1 Hz, 1H), 7.33 (d, J=1.5 Hz, 1H), 7.12 (d, J=1.2 Hz, 1H), 5.51 (s, 2H), 3.2o (t, J=6.9 Hz, 2H) 1.23 (t, J=6.9 Hz, 3H); LRMS Calcd 328.38. found 329
    • 5. Preparation of 3-Ethyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-3H-imidazo[4,5-c]pyridine
  • The mixture (2:1) of the cyclized 3-Ethyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-3H-imidazo[4,5-c]pyridine and the intermediate N-(3-ethylamino-pyridin-4-yl)-2-(2-thiazol-2-yl-imidazol-1-yl)-acetamide obtained from the above Me3Al-mediated coupling conditions is dissolved in 30 mL of acetic acid and heated at 115° C. overnight with stirring. The next day, most of the AcOH is evaporated off under reduced pressure. The residue is diluted with 100 mL of CH2Cl2, washed with NaHCO3, water, brine, dried over Na2SO4, and concentrated. The crude product is purified by chromatography, eluting with 3% MeOH—CH2Cl2 containing few drops of NH4OH to afford 3-ethyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-3H-imidazo[4,5-c]pyridine as an white solid. 1H NMR (300 MHz, CD3OD) δ 8.87 (s, 1H), 8.30 (d, J=5.7 Hz, 1H), 7.75 (d, J=3.3 Hz, 1H), 7.57 (d, J=4.8 Hz, 1H), 7.52 (d, J=3.3 Hz, 1H), 7.39 (d, J=1.5 Hz, 1H), 7.18 (d, J=1.5 Hz, 1H), 6.29 (s, 2H), 4.52 (q, J=7.5 Hz, 2H), 1.44 (t, J=7.5 Hz, 3H); LRMS Calcd 310.38 found 311; Anal. Calcd for CHNS: C, 58.05; H, 4.55; N, 27.08. Found C, 57.79; H, 4.38; N, 27.04.
    • Example 28 Synthesis of 3-ethyl-6-isopropyl-2-{[2-(1,3-thiazol-2-yl)-H-imidazol-1-yl]methyl}-3H-imidazo[4,5-c]pyridine and 1-ethyl-6-isopropyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-1H-imidazo[4,5-c]pyridine
  • Figure US20100267698A1-20101021-C00061
    • 1. Preparation of isobutyrylacetic acid
  • A mixture of ethyl isobutyrylacetate (20.47 g, 129 mmol) and 1.5 M aq NaOH (250 mL) is stirred at room temperature overnight. The solution is then cooled to 0° C. and acidified to pH 1-2 by the slow addition of conc. HCl (˜35 mL). The solution is then saturated with NaCl and extracted thrice with EtOAc and once with CHCl3. The combined extracts are dried over Na2SO4 and concentrated to 14.46 g (86%) of isobutyrylacetic acid as an oil. 1H NMR (CDCl3, 300 MHz) reveals a mixture of keto and enol forms (˜4:1), in favor of the keto form. Keto form: δ 3.57 (s, 2H), 2.73 (sept, J=6.9 Hz, 1H), 1.16 (d, J=6.9 Hz, 6H) ppm. Enol form: 11.85 (s, 1H), 5.03 (s, 1H), 2.44 (sept, J=6.9 Hz, 1H) 1.10 (d, J=6.9 Hz, 6H) ppm.
    • 2. Preparation of 4-hydroxy-3-isobutyryl-6-isopropyl-pyran-2-one
  • To a solution of the isobutyrylacetic acid (14.45 g, 111 mmol) in THF (200 mL) at room temperature under N2 is added CDI (19.8 g, 122 mmol) in one portion. The yellow solution is stirred at room temperature for 20 h and then concentrated in vacuo. The residue is dissolved in CH2Cl2 and washed with 10% aq HCl (100 mL) followed by H2O (50 mL). The aqueous washes are reextracted once with CH2Cl2, and the combined extracts are dried over Na2SO4 and concentrated to provide 4-hydroxy-3-isobutyryl-6-isopropyl-pyran-2-one as a yellow oil. This material is sufficiently pure to be used without further purification in the next reaction. 1H NMR (CDCl3, 300 MHz) δ 17.02 (s, 1H), 5.92 (s, 1H), 3.94 (sept, J=6.9 Hz, 1H), 2.72 (sept, J=6.9 Hz, 1H), 1.25 (d, J=6.9 Hz, 6H), 1.16 (d, J=6.9 Hz, 6H) ppm.
    • 3. Preparation of 4-hydroxy-6-isopropyl-pyran-2-one
  • A solution of 4-hydroxy-3-isobutyryl-6-isopropyl-pyran-2-one (10.26 g, 45.8 mmol) in conc. H2SO4 (40 mL) is stirred at 130° C. for 15 min. The dark reaction mixture is then cooled to 0° C., and crushed ice (˜200 g) is added with stirring. The resulting solution is extracted thrice with Et2O, and the combined extracts are dried over Na2SO4 and concentrated. The crude material is purified by flash chromatography on silica gel. Gradient elution with 3:2 hexanes-EtOAc, 1:1 EtOAc-hexanes, 2:1 EtOAc-hexanes, and 3:1 EtOAc-hexanes affords 4-hydroxy-6-isopropyl-pyran-2-one as a light yellow solid. 1H NMR (CDCl3, 300 MHz) δ 11.2 (br, 1H), 6.00 (d, J=1.8 Hz, 1H), 5.58 (d, J=1.8 Hz, 1H), 2.73 (sept, J=6.9 Hz, 1H), 1.22 (d, J=6.9 Hz, 1H) ppm.
    • 4. Preparation of 4-hydroxy-6-isopropyl-1H-pyridin-2-one
  • A mixture of 4-hydroxy-6-isopropyl-pyran-2-one (4.82 g, 31.3 mmol) in conc. NH4OH (15 mL) is stirred at 100° C. in a sealed tube for 4 h. The solution is then transferred to a recovery flask and concentrated in vacuo. Toluene is used to azeotropically remove any remaining water. 4-Hydroxy-6-isopropyl-1H-pyridin-2-one is obtained as a tan powder. 1H NMR (DMSO-d6, 300 MHz) δ 10.96 (br s, 1H), 10.36 (br s, 1H), 5.59 (d, J=2.1 Hz, 1H), 5.33 (d, J=2.1 Hz, 1H), 2.64 (sept, J=6.9 Hz, 1H), 1.11 (d, J=6.9 Hz, 6H) ppm.
    • 5. Preparation of 4-hydroxy-6-isopropyl-3-nitro-1H-pyridin-2-one
  • A solution of 4-hydroxy-6-isopropyl-1H-pyridin-2-one (1.76 g, 11.5 mmol) in 55-60 mL of 50% aq HNO3 is stirred at 70° C. for 2.5 h. After cooling, the reaction mixture is poured into ice-cold H2O (˜100 mL). The resulting solution is allowed to sit in the refrigerator for 3.5 days. The solid that precipitates is collected by filtration, washed with water, and dried to afford 4-hydroxy-6-isopropyl-3-nitro-1H-pyridin-2-one as yellow crystals. 1H NMR (DMSO-d6, 300 MHz) δ 12.30 (br s, 1H), 11.82 (br s, 1H), 5.82 (d, J=1.2 Hz, 1H), 2.71 (sept, J=6.9 Hz, 1H), 1.13 (d, J=6.9 Hz, 6H) ppm.
    • 6. Preparation of 4-chloro-6-isopropyl-3-nitro-1H-pyridin-2-one
  • To a suspension of 4-hydroxy-6-isopropyl-3-nitro-1H-pyridin-2-one (3.02 g, 15.2 mmol) in CH3CN (60 mL) at room temperature is added benzyltriethylammonium chloride (13.88 g, 61.0 mmol). The mixture is stirred at room temperature for 5-10 min, and then POCl3 (6.25 mL, 67.1 mmol) is added. The reaction mixture is stirred at room temperature for 15 min and then at reflux for 1 h. After cooling, the reaction mixture is concentrated in vacuo. The flask is then placed in an ice bath, and the residue treated with H2O (˜60 mL). The mixture is stirred for 4 h while slowly warming to rt. The solid that forms over this period of time is collected by filtration and then washed with H2O and a small amount of hexanes. 4-Chloro-6-isopropyl-3-nitro-1H-pyridin-2-one is obtained as a yellow solid. 1H NMR (CDCl3, 300 MHz) δ 13.13 (br s, 1H), 6.24 (s, 1H), 2.89 (sept, J=6.9 Hz, 1H), 1.31 (d, J=6.9 Hz, 6H) ppm.
    • 7. Preparation of 4-amino-6-isopropyl-3-nitro-1H-pyridin-2-one
  • A mixture of 4-chloro-6-isopropyl-3-nitro-1H-pyridin-2-one (700 mg, 3.23 mmol) in 6-7 μL of 7 N NH3 in MeOH is stirred at 100° C. in a sealed tube for 1.5 h. After cooling, the reaction mixture is concentrated in vacuo. The residue is suspended in H2O, filtered, washed with H2O, and dried, yielding 4-amino-6-isopropyl-3-nitro-1H-pyridin-2-one. 1H NMR (DMSO-d6, 300 MHz) δ 10.96 (br s, 1H), 8.15 (br s, 2H), 5.70 (d, J=0.9 Hz, 1H), 2.58 (sept, J=6.9 Hz, 1H), 1.11 (d, J=6.9 Hz, 6H) ppm.
    • 8. Preparation of 2-chloro-6-isopropyl-pyridin-4-ylamine
  • A mixture of 4-amino-6-isopropyl-3-nitro-1H-pyridin-2-one (555 mg, 2.81 mmol) in POCl3 (10 mL) is stirred at reflux under N2 for 1.5 h. After cooling, the solution is concentrated in vacuo. The reaction flask is then placed in an ice bath, and crushed ice (˜20 g) is added to the residue. The mixture is swirled vigorously for several minutes and then stirred at room temperature for 30 min. The mixture is then extracted with EtOAc. The extract is washed with an additional 20 mL of H2O. The aqueous washes are reextracted once with EtOAc, and the combined extracts are dried over Na2SO4 and concentrated, yielding 2-chloro-6-isopropyl-pyridin-4-ylamine as an orange oil. This material is used without further purification, 1H NMR (DMSO-d6, 300 MHz) δ 7.27 (br s, 2H), 6.65 (s, 1H), 2.77 (sept, J=6.9 Hz, 1H), 1.13 (d, J=6.9 Hz, 6H) ppm.
    • 9. Preparation of 6-isopropyl-pyridine-3,4-diamine hydrochloride
  • A solution of 2-chloro-6-isopropyl-pyridin-4-ylamine (684 mg, 3.17 mmol) in MeOH (10-15 mL) containing 10% Pd/C (˜70 mg) is stirred under an atmosphere of H2 (double stuffed balloon) for 5 h. The reaction mixture is then filtered through a pad of Celite using MeOH, and the filtrate is concentrated in vacuo. The residue is dissolved in a small amount of MeOH and further diluted with toluene. The solution is then concentrated in vacuo. This is repeated once more, affording 6-isopropyl-pyridine-3,4-diamine hydrochloride as an orange-yellow solid. 1H NMR (DMSO-d6, 300 MHz) δ 13.26 (br, 1H), 7.45, 7.40 (br m, 3H), 6.59 (s, 1H), 5.48 (br, 2H), 2.94 (sept, J=6.9 Hz, 1H), 1.19 (d, J=6.9 Hz, 6H) ppm.
    • 10. Preparation of 6-isopropyl-2-(2-thiazol-2-yl-imidazol-1-ylmethyl)-1H-imidazo[4,5-c]pyridine
  • To a mixture of 6-isopropyl-pyridine-3,4-diamine hydrochloride (265 mg, 1.41 mmol) in 1,2-dichloroethane (10 mL) at room temperature under N2 is added 2.0 M AlMe3 in toluene (2.12 mL, 4.24 mmol). The mixture is stirred at room temperature for 45 min and then treated with a solution of (2-thiazol-2-yl-imidazol-1-yl)-acetic acid methyl ester (252 mg, 1.13 mmol) in 1,2-dichloroethane (2 mL) via cannula, followed by a 1 mL rinse. The reaction mixture is then stirred at reflux for 20 h. After cooling, the reaction mixture is diluted with some MeOH (˜2 mL), followed by some water and then saturated aq NaHCO3. The resulting mixture is stirred vigorously for 15 min. The mixture is then extracted thrice with CHCl3 containing some (˜5) MeOH. The combined extracts are dried over Na2SO4 and concentrated. The crude residue is then triturated with CHCl3, and the resulting mixture filtered. The solid is washed with a small amount of CHCl3 and dried, affording N-(4-amino-6-isopropyl-pyridin-3-yl)-2-(2-thiazol-2-yl-imidazol-1-yl)-acetamide as an off-white solid. 1H NMR (DMSO-d6, 300 MHz) δ 9.52 (s, 1H), 7.86 (s, 1H), 7.85 (d, J=3.3 Hz, 1H), 7.75 (d, J=3.6 Hz, 1H)/, 7.39 (s, 1H), 7.04 (d, J=0.9 Hz, 1H), 6.45 (s, 1H), 5.65 (s, 2H), 5.38 (s, 2H), 2.74 (sept, J=6.9 Hz, 1H), 1.13 (d, J=6.9 Hz, 6H) ppm. The filtrate is concentrated and further purified by flash chromatography on silica gel. Elution with 20:1 CHCl3-MeOH followed by 15:1 CHCl3-MeOH affords 6-isopropyl-2-(2-thiazol-2-yl-imidazol-1-ylmethyl)-1H-imidazo[4,5-c]pyridine (160 mg, 44%). 1H NMR (CDCl3, 300 MHz) δ 12.23 (br, 1H), 8.97 (br, 1H), 7.94 (d, J=2.7 Hz, 1H), 7.43 (d, J=3.3 Hz, 1H), 7.23 (br, 1H), 7.20 (d, J=0.9 Hz, 1H), 7.04 (s, 1H), 5.82 (s, 2H), 3.14 (sept, J=6.9 Hz, 1H), 1.30 (d, J=6.9 Hz, 6H) ppm.
    • 11. Preparation of 3-ethyl-6-isopropyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-3H-imidazo[4,5-c]pyridine and 1-ethyl-6-isopropyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-1H-imidazo[4,5-c]pyridine
  • To a solution of 6-isopropyl-2-(2-thiazol-2-yl-imidazol-1-ylmethyl)-1H-imidazo[4,5-c]pyridine (160 mg, 0.493 mmol) in DMF (1.0 mL) at room temperature under N2 is added Cs2CO3 (168 mg, 0.518 mmol) followed by iodoethane (0.047 mL, 0.592 mmol). The mixture is stirred at room temperature for 1 h and then diluted with water and extracted twice with CH2Cl2. The combined extracts are dried over K2CO3 and concentrated. The residue is purified by preparative TLC, developing with 15:1 CHCl3-MeOH (+0.5% Et3N). The less polar (top) band affords 3-ethyl-6-isopropyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-3H-imidazo[4,5-c]pyridine. 1H NMR (CDCl3, 300 MHz) δ 8.70 (d, J=1.2 Hz, 1H), 7.83 (d, J=3.0 Hz, 1H), 7.53 (d, J=0.9 Hz, 1H), 7.39 (d, J=3.0 Hz, 1H), 7.18 (d, J=0.9 Hz, 1H), 7.14 (d, J=0.9 Hz, 1H), 6.32 (s, 2H), 4.32 (q, J=7.2 Hz, 2H), 3.18 (sept, J=6.9 Hz, 1H), 1.34 (d, J=6.9 Hz, 6H), 1.17 (t, J=7.2 Hz, 3H) ppm. The more polar (bottom) band affords 74 mg (43%) of 1-ethyl-6-isopropyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-1H-imidazo[4,5-c]pyridine. 1H NMR (CDCl3, 300 MHz) δ 9.01 (s, 1H), 7.83 (d, J=3.2 Hz, 1H), 7.38 (d, J=3.2 Hz, 1H), 7.15 (s, 1H), 7.12 (s, 1H), 7.09 (s, 1H), 6.32 (s, 2H), 4.21 (q, J=7.2 Hz, 2H), 3.17 (sept, J=6.8 Hz, 1H), 1.34 (d, J=6.8 Hz, 6H), 1.10 (t, J=7.2 Hz, 3H) ppm.
    • Example 29 Synthesis of 1-ethyl-6-isopropyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-1H-imidazo[4,5-c]pyridine
  • Figure US20100267698A1-20101021-C00062
    • 1. Preparation of 4-ethylamino-6-isopropyl-3-nitro-1H-pyridin-2-one
  • To a mixture of 4-chloro-6-isopropyl-3-nitro-1H-pyridin-2-one (Example 28, step 6) (500 mg, 2.31 mmol) in CH3CN at room temperature is added EtNH2.HCl (565 mg, 6.92 mmol) followed by Et3N (1.29 mL, 9.23 mmol). The reaction mixture is then stirred in a sealed tube at 100° C. for 1.5 h. After cooling, the reaction mixture is diluted with CH2Cl2 and washed with H2O (2×25 mL). The aqueous washes are reextracted once with CH2Cl2, and the combined extracts are dried over Na2SO4 and concentrated to provide 4-ethylamino-6-isopropyl-3-nitro-1H-pyridin-2-one as a yellow-orange solid. The material is sufficiently pure to be used without further purification. 1H NMR (DMSO-d6, 400 MHz) δ 11.1 (br, 1H), 8.93 (m, 1H), 5.73 (s, 1H), 3.37 (dq, J=7.4, 6.8 Hz, 2H), 2.67 (sept, J=6.8 Hz, 1H), 1.17 (t, J=7.4 Hz, 3H), 1.15 (d, J=6.8 Hz, 6H) ppm.
    • 2. Preparation of (2-chloro-6-isopropyl-3-nitro-pyridin-4-yl)-ethyl-amine
  • A mixture of 4-ethylamino-6-isopropyl-3-nitro-1H-pyridin-2-one (520 mg, 2.31 mmol) in POCl3 is stirred at reflux for 1.5 h. After cooling, the solution is concentrated in vacuo. The reaction flask is then placed in an ice bath and crushed ice is added to the residue. The mixture is swirled vigorously for a few minutes and then stirred at 0° C. for 1 h. The mixture is then extracted with EtOAc. The extract is washed with additional H2O (20 mL) and brine (20 mL). The extract is dried over Na2SO4 and concentrated to afford (2-chloro-6-isopropyl-3-nitro-pyridin-4-yl)-ethyl-amine as a yellow solid. The material is sufficiently pure to be used without further purification. 1H NMR (CDCl2, 400 MHz) δ 6.62 (br, 1H), 6.44 (s, 1H), 3.31 (m, 2H), 2.91 (sept, J=6.8 Hz, 1H), 1.33 (t, J=7.4 Hz, 3H), 1.26 (d, J=6.8 Hz, 6H) ppm.
    • 3. Preparation of N4-ethyl-6-isopropyl-pyridine-3,4-diamine hydrochloride
  • A solution of (2-chloro-6-isopropyl-3-nitro-pyridin-4-yl)-ethyl-amine (0.56 g, 2.30 mmol) in MeOH (20 mL) containing 10% Pd/C (˜50 mg) is stirred under and atmosphere of 12 for 3.5 h. The reaction mixture is then filtered through a pad of Celite using MeOH. The filtrate is concentrated in vacuo. Toluene is added to the residue and then removed in vacuo. This is repeated once more, yielding N4-ethyl-6-isopropyl-pyridine-3,4-diamine hydrochloride as a dark solid. 1H NMR (CD3OD, 400 MHz) δ 7.46 (s, 1H), 6.60 (s, 1H), 3.43 (q, J=7.4 Hz, 2H), 3.03 (sept, J=6.8 Hz, 1H), 1.35 (t, J=7.4 Hz, 3H), 1.33 (d, J=6.8 Hz, 6H) ppm.
    • 4. Preparation of 1-ethyl-6-isopropyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-1H-imidazo[4,5-c]pyridine
  • To a suspension of N4-ethyl-6-isopropyl-pyridine-3,4-diamine hydrochloride (126 mg, 0.582 mmol) in CH2Cl2 (5 mL) at room temperature under N2 is added 2.0 M AlMe3 in toluene (0.35 mL, 0.728 mmol). The dark solution is then stirred at room temperature for 45 min. Next, a solution of (2-Thiazol-2-yl-imidazol-1-yl)-acetic acid methyl ester (65 mg, 0.291 mmol) in CH2Cl2 (2 mL) is added via cannula followed by a 1 mL rinse. The reaction mixture is stirred at reflux overnight. After cooling, it is treated with MeOH (˜0.5 mL) and then diluted with 0.5 N NaOH and additional CH2Cl2. The mixture is stirred vigorously for 15 min and then extracted thrice with CH2Cl2 containing some (˜5%) MeOH. The combined extracts are dried over K2CO3 and concentrated to 156 mg of a crude mixture of the desired 1-ethyl-6-isopropyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-1H-imidazo[4,5-c]pyridine and N-(4-amino-6-isopropyl-pyridin-3-yl)-2-(2-thiazol-2-yl-imidazol-1-yl)-acetamide. This material is then dissolved in AcOH (6 mL), and the resulting solution is stirred at reflux for 5 h. After cooling, the reaction mixture is concentrated in vacuo. The residue is dissolved in CH2Cl2 and washed with 0.5 N NaOH. The aqueous layer is reextracted twice with CH2Cl2, and the combined extracts are dried over K2CO3 and concentrated. The crude material is purified by preparative TLC. Developing with 15:1 CHCl3 yields 1-ethyl-6-isopropyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-1H-imidazo[4,5-c]pyridine. 1H NMR (CDCl3, 400 MHz) δ 9.01 (s, 1H), 7.83 (d, J=3.2 Hz, 1H), 7.38 (d, J=3.2 Hz, 1H), 7.15 (s, 1H), 7.12 (s, 1H), 7.09 (s, 1H), 6.32 (s, 2H), 4.21 (q, J=7.2 Hz, 2H), 3.17 (sept, J=6.8 Hz, 1H), 1.34 (d, J=6.8 Hz, 6H), 1.10 (t, J=7.2 Hz, 3H) ppm.
    • Example 30 Synthesis of 1-ethyl-2-{[2-(1,3-thiazol-2-yl)-4-(trifluoromethyl)-1H-imidazol-1-yl]methyl}-1H-imidazo[4,5-c]pyridine
  • Figure US20100267698A1-20101021-C00063
    • 1. Preparation of 2-(4-trifluoromethyl-1H-imidazol-2-yl)-thiazole
  • Analogous to the published procedure (J. Med. Chem. 2000, 43, 2165-2175), 1,1-dibromo-3,3,3-trifluoroacetone (4.47 g, 16.6 mmol) is added to a solution of NaOAc (2.72 g, 33.1 mmol) in H2O (15 mL). The resulting solution is stirred at 100° C. for 30 min and then allowed to cool to rt. Next, a solution of 2-thiazolecarboxaldehyde (1.5 g, 13.3 mmol) in MeOH (15 mL), containing conc. NH4OH (5 mL), is added. The reaction mixture is then stirred at room temperature for 20 h. It is then filtered, the solid washed with H2O, and dried. 2-(4-Trifluoromethyl-1H-imidazol-2-yl)-thiazole is obtained as an off-white fluffy solid. 1H NMR (DMSO-d6, 400 MHz) δ 13.81 (br, 1H), 7.97 (d, J=3.2 Hz, 1H), 7.94 (s, 1H), 7.87 (d, J=3.2 Hz, 1H) ppm.
    • 2. Preparation of (2-thiazol-2-yl-4-trifluoromethyl-imidazol-1-yl)-acetic acid methyl ester
  • To a suspension of NaH (109 mg of a 60% dispersion in mineral oil, 2.74 mmol) in THF (10 mL) at 0° C. under N2 is added a solution of 2-(4-trifluoromethyl-1H-imidazol-2-yl)-thiazole (500 mg, 2.28 mmol) in THF (4 mL) via cannula, followed by a 1 mL rinse. The mixture is stirred at 0° C. for 30 min and then treated with methyl bromoacetate (0.28 mL, 2.97 mmol). The reaction mixture is stirred overnight while slowly warming to rt. It is then diluted with H2O (10 mL) and the resulting mixture stirred vigorously for 5 min. The mixture is further diluted with some brine and then extracted twice with EtOAc. The combined extracts are dried over Na2SO4 and concentrated. The crude material is purified by flash chromatography on silica gel. Elution with 4:1 hexanes-EtOAc followed by 3:1 hexanes-EtOAc affords unreacted 2-(4-trifluoromethyl-1H-imidazol-2-yl)-thiazole as well as (2-thiazol-2-yl-4-trifluoromethyl-imidazol-1-yl)-acetic acid methyl ester. 1H NMR (CDCl3, 400 MHz) δ 7.82 (d, J=3.2 Hz, 1H), 7.40 (d, J=3.2 Hz, 1H), 7.35 (s, 1H), 5.41 (s, 2H), 3.77 (s, 3H) ppm.
    • 3. Preparation of 1-ethyl-2-[{2-(1,3-thiazol-2-yl)-4-(trifluoromethyl)-1H-imidazol-1-yl]methyl}-1H-imidazo[4,5-c]pyridine
  • To a mixture of N4-ethyl-pyridine-3,4-diamine (94 mg, 0.687 mmol) in CH2Cl2 (5 mL) at room temperature under N2 is added 2.0 M AlMe3 in toluene (0.43 mL, 0.858 mmol), and the resulting mixture is stirred at room temperature for 1 h. Next, a solution of (2-thiazol-2-yl-4-trifluoromethyl-imidazol-1-yl)-acetic acid methyl ester (100 mg, 0.343 mmol) in CH2Cl2 is added via cannula. The reaction mixture is then stirred at reflux overnight. After cooling to rt, it is treated with MeOH (˜1 mL). The mixture is further diluted with 0.5 N aq NaOH (10 mL) and then stirred vigorously for 30 min. The mixture is then extracted thrice with CH2Cl2 containing some (˜5%) MeOH, and the combined extracts are dried over K2CO3 and concentrated. The crude mixture of 1-ethyl-2-{[2-(1,3-thiazol-2-yl)-4-(trifluoromethyl)-1H-imidazol-1-yl]methyl}-1H-imidazo[4,5-c]pyridine and N-(4-amino-pyridin-3-yl)-2-(2-thiazol-2-yl-4-trifluoromethyl-imidazol-1-yl)-acetamide is dissolved in AcOH (6 mL), and the resulting solution stirred at reflux overnight. The reaction mixture is then concentrated, and the residue is dissolved in CH2Cl2 and washed with 0.5 N aq NaOH. The aqueous layer is reextracted once with CH2Cl2, and the combined extracts are dried over K2CO3 and concentrated. The crude residue is purified by preparative TLC. Developing with 15:1 CHCl3-MeOH affords 1-ethyl-2-{[2-(1,3-thiazol-2-yl)-4-(trifluoromethyl)-1H-imidazol-1-yl]methyl}-1H-imidazo[4,5-c]pyridine. 1H NMR (CDCl3, 400 MHz) δ 9.10 (s, 1H), 8.47 (d, J=6.0 Hz, 1H), 7.88 (d, J=3.2 Hz, 1H), 7.58 (s, 1H), 7.48 (d, J=3.2 Hz, 1H), 7.31 (d, J=5.6 Hz, 1H), 6.37 (s, 2H), 4.31 (q, J=7.2 Hz, 2H), 1.24 (t, J=7.2 Hz, 3H) ppm.
    • Example 31 Synthesis of 3-ethyl-2-{[2-(6-fluoropyridin-2-yl)-1H-imidazol-1-yl]methyl}-6-propyl-3H-imidazo[4,5-c]pyridine
  • Figure US20100267698A1-20101021-C00064
    • 1. Preparation of N-(4-amino-6-propyl-pyridin-3-yl)-acetamide
  • To a mixture of 6-propyl-pyridine-3,4-diamine hydrochloride (prepared from ethyl butyrylacetate as described in Example 28, steps 1-9) (187 mg, 1.00 mmol) in CH2Cl2 (4 mL) at 0° C. under N2 is added pyridine (0.24 mL, 3.00 mmol) followed by DMF (2 mL) (for solubility). Next, AC20 (0.104 mL, 1.1 mmol) is added. The reaction mixture is stirred for 3 h while slowly warming to rt. It is then poured into saturated aq NaHCO3 and extracted thrice with CH2Cl2. The combined extracts are dried over K2CO3 and concentrated to 114 mg of crude N-(4-amino-6-propyl-pyridin-3-yl)-acetamide, which was used without further purification. 1H NMR (CDCl3, 300 MHz) δ 8.73 (br, 1H), 8.00 (s, 1H), 8.43 (s, 1H), 4.63 (br, 2H), 2.54 (m, 2H), 2.13 (s, 3H), 1.63 (sextet, J=7.2 Hz, 2H), 0.89 (t, J=7.2 Hz, 3H) ppm. Electrospray MS: m/z 194 [M+1].
    • 2. Preparation of N3-ethyl-6-propyl-pyridine-3,4-diamine
  • A solution of the crude N-(4-amino-6-propyl-pyridin-3-yl)-acetamide (114 mg, 0.59 mmol) in THF (2.5 mL) at 0° C. under N2 is treated with 0.5 M EtNMe2.AlH3 in toluene (2.4 mL, 1.18 mmol). The reaction mixture is stirred for 4 h while slowly warming to rt, whereupon it is treated with additional 0.5 M EtNMe2.AlH3 in toluene (2.4 mL, 1.18 mmol). After stirring at room temperature overnight, the reaction mixture is cooled to 0° C. and treated with moist Na2CO3. The mixture is diluted with CH2Cl2 and stirred vigorously for 15 min. It is then filtered through a pad of Celite, and the filtrate concentrated. The crude material is partially purified by preparative thin layer chromatography, developing with 15:1 CHCl3-MeOH (+1% Et3N). The band containing the desired N3-ethyl-6-propyl-pyridine-3,4-diamine does not separate well from the approximately 10% of the slightly more polar, unreacted N-(4-amino-6-propyl-pyridin-3-yl)-acetamide. Both bands are collected, and the mixture is carried into the next reaction. 1H NMR (CDCl3, 300 MHz) δ 7.74 (s, 1H), 6.42 (s, 1H), 4.10 (br, 1H), 3.67 (m, 1H), 3.10 (q, J=7.2 Hz, 2H), 2.56 (m, 2H), 1.67 (sextet, J=7.2 Hz, 2H), 1.25 (t, J=7.2 Hz, 3H), 0.91 (t, J=7.2 Hz, 3H) ppm. Electrospray MS: m/z 180 [M+1].
    • 3. Preparation of 3-ethyl-2-{[2-(6-fluoropyridin-2-yl)-1H-imidazol-1-yl]methyl}-6-propyl-3H-imidazo[4,5-c]pyridine
  • A solution of N3-ethyl-6-propyl-pyridine-3,4-diamine (82 mg, ˜0.45 mmol, contaminated with ˜10% of N-(4-amino-6-propyl-pyridin-3-yl)-acetamide) in 1,2-dichloroethane (4 mL) at room temperature under N2 is treated with 2.0 M AlMe3 (0.69 mL, 1.37 mmol). The resulting solution is stirred at room temperature for 30 min and then treated with a solution of [2-(6-fluoro-pyridin-2-yl)-imidazol-1-yl]-acetic acid methyl ester (97 mg, 0.41 mmol) in 1,2-dichloroethane via cannula, followed by a 1 ml rinse. The reaction mixture is then stirred at reflux overnight. After cooling, it is treated with MeOH (˜2 mL) and then further diluted with saturated aq NaHCO3 (˜10 mL) and some H2O. The mixture is stirred vigorously for 15 min and then extracted thrice with CHCl3 containing some (˜5%) MeOH. The combined extracts are dried over K2CO3 and concentrated. The crude material is purified by preparative thin layer chromatography. Developing with 15:1 CHCl3-MeOH yields 3-ethyl-2-{[2-(6-fluoropyridin-2-yl)-1H-imidazol-1-yl]methyl}-6-propyl-3H-imidazo[4,5-c]pyridine as an off-white solid. 1H NMR (CDCl3, 300 MHz) δ 8.70 (d, J=0.9 Hz, 1H), 8.17 (dd, J=8.0, 2.6 Hz, 1H), 7.89 (q, J=7.8 Hz, 1H), 7.47 (s, 1H), 7.22 (d, J=0.9 Hz, 1H), 7.18 (d, J=1.5 Hz, 1H), 6.88 (dd, J=8.6, 2.9 Hz, 1H), 6.27 (s, 2H), 4.42 (q, J=7.2 Hz, 2H), 2.86 (m, 2H), 1.78 (sextet, J=7.2 Hz, 2H), 1.30 (t, J=7.2 Hz, 3H), 0.97 (t, J=7.2 Hz, 3H) ppm. Electrospray MS: m/z 365 [M+1].
    • Example 32 Synthesis of 1-ethyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-6-(trifluoromethyl)-1H-imidazo[4,5-c]pyridine
  • Figure US20100267698A1-20101021-C00065
    • 1. Preparation of 5-Nitro-2-trifluoromethyl-pyridin-4-ol
  • 25 mL of fuming H2SO4 under cold conditions (0° C.) is added dropwise to a stirred solution of 4-hydroxy-2-trifluoromethylpyridine [Chemistry of Heterocyclic Compounds 1997, 33, 995-996] (4 g, 24.5 mmol) in concentrated H2SO4 (10 mL). HNO3 (fuming, 90%, 25 mL) is added carefully to the above mixture with caution to keep the offset of any exotherm under control, and the reaction is allowed to warm to room temperature slowly. After heating at 120° C. for 6 h with stirring, the resulting mixture is cooled to room temperature, poured into ice-cold water maintaining the pH around 1 with the addition of 10N NaOH solution, and then extracted with CHCl3. The combined organic phase is washed successively with saturated aqueous NaHCO3, water, brine, dried over Na2SO4, and concentrated in vacuo to afford 5-nitro-2-trifluoromethyl-pyridin-4-ol as an yellow solid. 1H NMR (300 MHz, CDCl3) δ 9.33 (s, 1H), 7.50 (s, 1H).
    • 2. Preparation of 4-Chloro-5-nitro-2-trifluoromethyl-pyridine
  • The crude 5-nitro-2-trifluoromethyl-pyridin-4-ol (3.4 g, 16.3 mmol) was treated with PCl5 (5.2 g, 24.97 mmol) and POCl3 (7 mL, 24.49 mmol). The resulting mixture is heated at 80° C. for 18 h, cooled and poured into ice-cold water, and extracted with CH2Cl2. The combined organic phase is washed successively with saturated aqueous NaHCO3, water, brine, dried over Na2SO4, and concentrated in vacuo to afford 4-Chloro-5-nitro-2-trifluoromethyl-pyridine as an yellow oil. 1H NMR (300 MHz, CDCl3): δ 9.17 (s, 1H) 7.9 (s, 1H).
    • 3. Preparation of Ethyl-(5-nitro-2-trifluoromethyl-pyridin-4-yl)-amine
  • K2CO3 (4.15 g, 30.01 mmol), and 2.0 M solution of EtNH2 (15 mL, 30.01 mmol) in THF are added to a solution of 4-chloro-5-nitro-2-trifluoromethyl-pyridine (3.4 g, 15.0 mmol) in acetonitrile (30 mL) under cold conditions. The reaction is complete after stirring at 0° C. for 2 h. Usual work up affords ethyl-(5-nitro-2-trifluoromethyl-pyridin-4-yl)-amine as an yellow-orange viscous oil. 1H NMR (300 MHz, CDCl3) δ 9.24 (s, 1H) 7.09 (s, 1H), 3.45 (t, J=7.2 Hz, 2H), 1.42 (t, J=7.2 Hz, 3H).
    • 4. Preparation of N4-Ethyl-6-trifluoromethyl-pyridine-3,4-diamine
  • Ethyl-(5-nitro-2-trifluoromethyl-pyridin-4-yl)-amine is hydrogenated under usual conditions to afford N-ethyl-6-trifluoromethyl-pyridine-3,4-diamine. 1H NMR (300 MHz, CD3OD): δ 7.71 (s, 1H), 6.75 (s, 1H), 3.28 (t, J=6.9 Hz, 2H), 1.31 (t, J=6.9 Hz, 3H).
    • 5. Preparation of 2-Chloromethyl-1-ethyl-6-trifluoromethyl-1H-imidazo[4,5-c]pyridine
  • The HCl salt of ethyl 2-chloro-acetimidate (3.90 g, 24.58 mmol) is added to a solution of N-ethyl-6-trifluoromethyl-pyridine-3,4-diamine (1.44 g, 7.02 mmol) in EtOH (20 mL) and refluxed for 3 h. The reaction mixture is cooled to room temperature and concentrated under reduced pressure. The residue is diluted with CH2Cl2, washed with NaHCO3 solution, dried over Na2SO4, and concentrated to obtain 2-chloromethyl-1-ethyl-6-trifluoromethyl-1H-imidazo[4,5-c]pyridine. 1H NMR (300 MHz, CDCl3): δ 9.14 (s, 1H), 7.66 (s, 1H), 7.26 (s, 1H), 4.87 (s, 2H), 4.40 (t, J=7.5 Hz, 2H), 1.58 (t, J=7.5 Hz, 3H).
    • 6. Preparation of 1-ethyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-6-(trifluoromethyl)-1H-imidazo[4,5-c]pyridine
  • A solution of 2-Chloromethyl-1-ethyl-6-trifluoromethyl-1H imidazo[4,5-c]pyridine (1 g, 3.8 mmol) in anhydrous DMF (15 mL) were added K2CO3 (2.68 g, 19 mmol), and 2-(1H-imidazol-2-yl)-thiazole (555 mg, 3.8 mmol) and stirred at room temperature for 48 h. The crude residue after usual work up was purified eluting with 5% MeOH—CH2Cl2 containing few drops of NH4OH to afford 1-ethyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-6-(trifluoromethyl)-1H-imidazo[4,5-c]pyridine (800 mg, 80%); 1H NMR (CD3OD): δ 8.83 (s, 1H), 8.14 (s, 1H), 7.71 (d, J=2.7 Hz, 1H), 7.53 (d, J=2.7 Hz, 1H), 7.45 (d, J=0.9 Hz, 1H), 7.19 (d, J=0.9 Hz, 1H), 6.30 (s, 2H), 4.54 (q, J=5.4 Hz, 2H), 1.46 (t, J=5.4 Hz, 3H); m/z 379 [M+1]
    • Example 33 Synthesis of 3-ethyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-6-(trifluoromethyl)-3H-imidazo[4,5-c]pyridine
  • Figure US20100267698A1-20101021-C00066
    • 1. Preparation of 5-Nitro-2-trifluoromethyl-pyridin-4-ylamine
  • Ammonia gas is bubbled through a solution of 2-trifluoromethyl-4-chloro-5-nitro-pyridine (4.12 g, 18.23 mmol) in anhydrous THF at room temperature for 3 h. Removal of solvent under reduced pressure affords 5-nitro-2-trifluoromethyl-pyridin-4-ylamine (3.6 g, 96%); 1H NMR (300 MHz, CDCl3) δ 7.15 (s, 1H), 9.15 (s, 1H).
    • 2. Preparation of 6-Trifluoromethyl-pyridine-3,4-diamine
  • Hyrogenation as described in the previous examples provides 6-trifluoromethyl-pyridine-3,4-diamine. 1H NMR (300 MHz, CDCl3) δ 6.95 (s, 1H), 8.01 (s, 1H).
    • 3. Preparation of N3-ethyl-6-trifluoromethyl-pyridine-3,4-diamine
  • Cesium carbonate (3.1 g, 26.88 mmol) and ethyl iodide (1.76 g, 10.75 mmol) is added to a solution of 6-trifluoromethyl-pyridine-3,4-diamine (1.05 g, 8.96 mmol) in anhydrous DMF (12 mL). The mixture is heated in a sealed tube for 18 h, then cooled to room temperature, and diluted with EtOAc and water. Usual work up and purification by column chromatography, eluting with EtOAc-hexanes provides pure N-Ethyl-6-trifluoromethyl-pyridine-3,4-diamine. 1H NMR (300 MHz, CDCl3) δ 7.95 (s, 1H); 6.95 (s, 1H), 3.21 (q, J=5.4 Hz, 2H), 1.35 (t, J=5.4 Hz, 3H).
    • 4. Preparation of 2-Chloromethyl-3-ethyl-6-trifluoromethyl-3H-imidazo[4,5-c]pyridine
  • 2-Chloromethyl-3-ethyl-6-trifluoromethyl-3H-imidazo[4,5-c]pyridine is made from N3-Ethyl 6-Trifluoromethyl-pyridine-3,4-diamine following the procedure described previously for the preparation of 2-Chloromethyl-1-ethyl-6-trifluoromethyl-1H-imidazo[4,5-c]pyridine. 1H NMR (300 MHz, CDCl3) δ 8.89 (s, 1H), 8.04 (s, 1H), 4.87 (s, 2H), 4.45 (t, J=7.2 Hz, 2H), 1.59 (t, J=7.2 Hz, 3H)
    • 5. Preparation of 3-ethyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-6-(trifluoromethyl)-3H-imidazo[4,5-c]pyridine
  • As described previously for the preparation of 1-Ethyl-2-(2-thiazol-2-yl-imidazol-1-ylmethyl)-6-trifluoromethyl-1H-imidazo[4,5-c]pyridine, nucleophilic displacement of the 2-chloromethyl-3-ethyl-6-trifluoromethyl-3H-imidazo[4,5-c]pyridine with the same 2-(1H-imidazol-2-yl)-thiazole followed by usual work provides 3-ethyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-6-(trifluoromethyl)-3H-imidazo[4,5-c]pyridine. 1H NMR (CD3OD) δ 9.02 (s, 1H), 7.89 (s, 1H), 7.70 (d, J=3 Hz, 1H), 7.52 (d, J=3.3 Hz, 1H), 7.46 (d, J=1.5 Hz, 1H), 7.19 (d, J=1.5 Hz, 1H), 6.29 (s, 2H), 4.56 (q, J=7.5 Hz, 2H), 1.52 (t, J=7.5 Hz, 3H) m/z 379 [M+1]
    • Example 34 Synthesis of 3-ethyl-2-[(2-pyrimidin-2-yl-1H-imidazol-1-yl)methyl]-3H-imidazo[4,5-c]pyridine-4-carbonitrile
  • Figure US20100267698A1-20101021-C00067
    • 1. Preparation of 3-Ethylamino-4-nitro-pyridine-2-carbonitrile
  • TMSCN (1.1 ml, 8.33 mmol) and dimethyl amino carbamoyl chloride (0.76 mL, 8.33 mmoL) are added to a solution of 3-ethylamino-4-nitro-pyridine-1-oxide (1 g, 6.94 mmol) in anhydrous DMF (10 mL) were added. The reaction mixture is stirred overnight at 80° C., cooled to room temperature, and diluted with CH2Cl2 and water. The combined organic layer is washed with water, brine, dried over Na2SO4, and concentrated. The residue is chromatographed eluting with 40% EtOAc-hexanes to afford 3-ethylamino-4-nitro-pyridine-2-carbonitrile as a yellow solid. 1H NMR (300 MHz, CD3OD): δ 7.82 (d, J=4.5 Hz, 1H), 7.50 (d, J=4.5 Hz, 1H), 3.70 (q, J=7.2 Hz, 2H), 1.39 (t, J=7.2 Hz, 3H).
    • 2. Preparation of 4-Amino-3-ethylamino-pyridine-2-carbonitrile
  • Hydrogenaton of 3-Ethylamino-4-nitro-pyridine-2-carbonitrile under conditions previously described provides 4-Amino-3-ethylamino-pyridine-2-carbonitrile. 1H NMR (300 MHz, CD3OD): δ 7.75 (d, J=5.4 Hz, 1H), 6.66 (d, J=5.4 Hz, 1H), 3.35 (q, J=7.2 Hz, 2H), 1.29 (t, J=7.2 Hz, 3H).
    • 3. Preparation of 2-Chloromethyl-3-ethyl-3H-imidazo[4,5-c]pyridine-4-carbonitrile
  • 2-Chloromethyl-3-ethyl-3H-imidazo[4,5-c]pyridine-4-carbonitrile is prepared from the reaction of 4-amino-3-ethylamino-pyridine-2-carbonitrile and 2-chloro acetimidate following the previously described procedure. 1H NMR (300 MHz, CDCl3) δ 8.55 (d, J=5.4 Hz, 1H), 7.84 (d, J=5.4 Hz, 1H), 4.98 (s, 2H), 3.41 (q, J=7.5 Hz, 2H), 1.23 (t, J=7.5 Hz, 3H).
    • 4. Preparation of 3-ethyl-2-[(2-pyrimidin-2-yl-1H-imidazol-1-yl)methyl]-3H-imidazo[4,5-c]pyridine-4-carbonitrile
  • 3-ethyl-2-[(2-pyrimidin-2-yl-1H-imidazol-1-yl)methyl]-3H-imidazo[4,5-c]pyridine-4-carbonitrile is obtained from 2-chloromethyl-3-ethyl-3H-imidazo[4,5-c]pyridine-4-carbonitrile by nucleophilic displacement with 2-(1H-Imidazol-2-yl)-pyrimidine, as described previously, followed by usual work up. 1H NMR (300 MHz, CD3OD): δ 8.70 (d, J=5.1 Hz, 2H), 8.41 (d, J=5.4 Hz, 1H), 7.73 (d, J=5.1 Hz, 1H), 7.52 (d, J=1.2 Hz, 1H), 7.31 (d, J=1.2 Hz, 1H), 7.27 (t, J=4.5 Hz, 1H), 6.35 (s, 2H), 4.75 (q, J=7.5 Hz, 2H), 1.63 (t, J=7.2 Hz, 3H); m/z 331 [M+1].
    • Example 35 Synthesis of 1-(2-{[2-(6-fluoropyridin-2-yl)-1H-imidazol-1-yl]methyl}-1-propyl-1H-imidazo[4,5-c]pyridin-4-yl)ethanone
  • Figure US20100267698A1-20101021-C00068
  • To the mixture of H2O (5 mL) and CH2Cl2 (5 mL) is added 1-Propyl-2-{[2-(2-fluoropyrid-6-yl)-1H-imidazol-1-yl]methyl}-5-aza-1H-benzimidazole (168 mg, 0.5 mmol), pyruvic acid (132 mg, 1.5 mmol), silver nitrate (7 mg, 0.04 mmol), (NH4)2S2O8 (342 mg, 1.5 mmol), and sulfuric acid (98%, 100 mg, 1.0 mmol). The mixture is heated to 40° C. for 2 hr, then cooled to room temperature. The aqueous solution is neutralized to pH 8 with saturated sodium bicarbonate solution and extracted with CH2Cl2. The combined organic layers are dried over Na2SO4 and solvent is removed to give a brown solid. Purification by preparative TLC provides ‘1-(2-({[2-(6-fluoropyridin-2-yl)-1H-imidazol-1-yl]methyl}-1-propyl-1H-imidazo[4,5-c]pyridin-4-yl)ethanone as white solid. 1H NMR (CDCl3) δ 8.48 (d, 1H), 8.12 (dd, 1H), 7.88 (q, 1H), 7.44 (d, 1H), 7.23 (d, 1H), 7.11 (d, 1H), 6.88 (dd, 1H), 6.40 (s, 2H), 4.22 (q, 2H), 2.84 (s, 3H), 1.58 (m, 2H), 0.74 (t, 3H).
    • Example 36 Synthesis of 1-(3-ethyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-3H-imidazo[4,5-c]pyridin-6-yl)ethanone
  • Figure US20100267698A1-20101021-C00069
    • 1. Preparation of 2,5-Dichloro-pyridine 1-oxide
  • A mixture of 2,5-Dichlropyridine (30 g, 0.2 mmol) and 3-Chloroperoxybenzoic acid (70%, 50 g, 0.2 mmol) in 500 mL of Dichloroethane is heated at 50° C. overnight. After cooling to −30° C., NH3 gas is bubbled into the reaction mixture for 5 minutes. The mixture is filtered, the solid is washed with dichloroethane (50 mL), and the filtrate is concentrated to a solid. The solid is mixed with 200 mL of Hexane and heated at reflux for 30 minutes. After cooling, the precipitate is collected by filtration, and dried to give the titled compound as a tan solid. 1H NMR (CDCl3): δ 8.40 (d, 1H), 7.44 (d, 1H), 7.22 (t, 1H).
    • 2. Preparation of 2,5-dichloro-4-nitro-pyridine 1-oxide
  • At 0° C., 30% oleum (13 mL) is added slowly to fuming HNO3 (22 mL) and the resulting mixture is added slowly to the solution of 2,5-dichloro-pyridine 1-oxide (5.2 g, 31 mmol) in concentrated H2SO4. After addition, the reaction mixture is warmed to room temperature over 1 hr, then heated to 80° C. for 2 hr. After cooling to room temperature, the mixture is poured into ice (120 g) and precipitate is formed gradually. The solid is collected by filtration and washed with water. The product is obtained as yellow solid. 1H NMR (CDCl3) δ 8.46 (s, 1H), 8.25 (s, 1).
    • 3. Preparation of (6-Chloro-4-nitro-1-oxy-pyridin-3-yl)-ethylamine
  • To a solution of 2,5-Dichloro-4-nitro-pyridine 1-oxide (7 g, 33 mmol) in 100 mL of THF is added a solution of Ethylamine (2M, 50 mL) dropwise at ° C. The reaction mixture is warmed to room temperature and stirred overnight. The volatiles are evaporated in vacuo. The residue is purified on a silica gel column with 1% CH2Cl2 as eluent to give the title compound as an orange solid. 1H NMR (CDCl3): δ 8.24 (s, 1H), 8.06 (s, 1H), 7.62 (br, 1H), 3.25 (q, 2H), 1.38 (t, 3H).
    • 4. Preparation of 1-(5-ethylamino-4-nitro-pyridin-2-yl)-ethanone
  • (6-Chloro-4-nitro-1-oxy-pyridin-3-yl)ethyl-amine (700 mg, 3.2 mmol), tributyl-(1-ethoxy-vinyl)-stannane (1.75 g, 4.9 mmol), PdCl2(PPh3)2 (350 mg, 0.5 mmol), and toluene (35 mL)o are added to a sealed tube; the mixture is degassed for 30 min. The reaction is heated to 75° C. for 16 hr and then cooled to room temperature. 6N HCl solution (20 mL) is added to the mixture is added and the reaction is stirred at room temperature for 1 hr. The reaction mixture is filtered through celite and the aqueous layer is neutralized to basic with saturated NaHCO3. The aqueous layer is extracted with ethyl acetate and the combined organic layers are dried over Na2SO4. The solvent is removed in vacuo. Purification by flash column provides the product as orange solid. 1H NMR (CDCl3) δ 8.70 (s, 1H), 8.48 (s, 1H), 7.96 (br, 1H), 3.55 (m, 2H), 2.65 (s, 3H), 0.92 (t, 3H). LRMS 210.1 (MH+).
    • 5. Preparation of 1-(4-amino-5-ethylamino-pyridin-2-yl)-ethanone
  • A mixture of 1-(5-ethylamino-4-nitro-pyridin-2-yl)-ethanone (310 mg, 1.5 mmol) and 10% Pd/C (50 mg) in ethanol (10 mL) is stirred under hydrogen for 1 hr. The mixture is filtered through Celite and concentrated in vacuo to give the product as yellow solid. 1H NMR (CD3OD) δ 7.61 (s, 1H), 7.30 (s, 1H), 3.26 (q, 2H), 2.53 (s, 3H), 0.90 (t, 3H). LRMS 180.5 (MH+).
    • 6. Preparation of 1-(3-ethyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-3H-imidazo[4,5-c]pyridin-6-yl)ethanone
  • A mixture of 1-(4-amino-5-ethylamino-pyridin-2-yl)-ethanone (54 mg, 0.3 mmol), (2-thiazol-2-yl-imidazol-1-yl)-acetic acid (61 mg, 0.3 mmol), EDCI (60 mg, 0.3 mmol) and pyridine (2 mL) is stirred at room temperature for 72 hr and then poured into water. The aqueous layer is extracted with ethyl acetate and the combined organic layers are dried over Na2SO4. Solvent is evaporated in vacuo and the residue is taken up in acetic acid (5 mL). The mixture is heated to reflux for 16 hr and saturated NaHCO3 solution is added to neutralize the reaction. The aqueous solution is extracted with ethyl acetate and the combined organic layers are dried over Na2SO4. Solvent is removed in vacuo and purification by preparative TLC provides the product as white solid. 1H NMR (CDCl3) δ 8.79 (d, 2H), 8.47 (d, 1H), 7.83 (d, 1H), 7.40 (d, 1H), 7.19 (d, 1H), 7.15 (d, 1H), 6.39 (s, 2H), 4.02 (q, 2H), 2.77 (s, 3H), 1.86 (t, 3H). LRMS 353.2 (MH+).
    • Example 37 Synthesis of 1-Ethyl-2-(2-thiazol-2-yl-2H-pyrazol-3-ylmethyl)-1H-imidazo[4,5-c]pyridine
  • Figure US20100267698A1-20101021-C00070
    • 1. Preparation of (2-Thiazol-2-yl-2H-pyrazol-3-yl)-acetic acid ethyl ester
  • A mixture of thiazol-2-ylhydrazine (2.2 g, 19.1 mmol) and 3,5,5-triethoxy-pent-2-enoic acid ethyl ester (70% purity, 7.1 g, 19.1 mmol) is refluxed in acetic acid overnight. Acetic acid is removed. To the residue is added NaHCO3 (aq.) (40 mL) and ethyl acetate (100 mL). The insoluble material is filtered. The organic layer is dried and solvent removed. The crude is subjected to column separation (hexane/ethyl acetate 3:1) to give (2-thiazol-2-yl-2H-pyrazol-3-yl)-acetic acid ethyl ester as an oil.
    • 2. Preparation of 1-Ethyl-2-(2-thiazol-2-yl-2H-pyrazol-3-ylmethyl)-1H-imidazo[4,5-c]pyridine
  • Trimethylaluminum (2M in toluene) (1.4 mL, 2.85 mmol) is added dropwise under N2 to a solution of N4-Ethyl-pyridine-3,4-diamine (156 mg, 1.14 mmol) in DCM (5 mL). The mixture is stirred at room temperature for 1 hour. A solution of thiazol-2-yl-2H-pyrazol-3-yl)-acetic acid ethyl ester (270 mg, 1.14 mmol) in DCM (2 mL) is added. The mixture is reluxed for 25 hours. On cooling, the reaction is quenched with water added dropwise and DCM (20 mL) is added. The organic layer is separated and the aqueous layer is extracted with DCM (3×30 mL). The combined organic layers are dried and solvent removed. Acetic acid (10 ml) is added to the residue. The solution is refluxed for 3.5 h. Acetic acid is removed in vacuo and the residue is purified by PTLC (10% methanol in DCM) to give 1-Ethyl-2-(2-thiazol-2-yl-2H-pyrazol-3-ylmethyl)-1H-imidazo[4,5-c]pyridine as a solid. 1H NMR (CDCl3) δ 9.00 (s, 1H), 8.43 (d, 1H), 7.63 (d, 1H), 7.43 (d, 1H), 7.15 (d, 1H) 7.06 (d, 1H), 6.25 (d, 1H), 4.99 (s, 2H), 4.25 (q, 2H), 1.36 (t, 3H).
    • Example 38 Synthesis of 1-Ethyl-2-[1-(2-thiazol-2-yl-2H-pyrazol-3-yl)-ethyl]-1H-imidazo[4,5-c]pyridine
  • Figure US20100267698A1-20101021-C00071
    • 1. Preparation of 2-(2-Thiazol-2-yl-2H-pyrazol-3-yl)-propionic acid ethyl ester
  • A mixture of (2-thiazol-2-yl-2H-pyrazol-3-yl)-acetic acid ethyl ester (100 mg, 0.42 mmol), methyl iodide (66 mg, 0.46 mmol) and cesium carbonate (151 mg, 0.46 mmol) in DMF (6 mL) is stirred at room temperature overnight. The reaction is quenched with NH4Cl (aq.) (6 mL) and ethyl acetate (30 mL). The organic layer is separated and the aqueous layer is extracted with ethyl acetate (3×30 mL). The combined organic layers are dried and the solvent removed. The crude is purified by PTLC (hexane/ethyl acetate 3:1) to give 2-(2-thiazol-2-yl-2H-pyrazol-3-yl)-propionic acid ethyl ester as an oil.
    • 2. Preparation of 1-Ethyl-2-[1-(2-thiazol-2-yl-2H-pyrazol-3-yl)-ethyl]-1H-imidazo[4,5-c]pyridine
  • Trimethylaluminum (2N in toluene)(0.5 mL, 1 mmol)) is added dropwise under N2 to a solution of N4-Ethyl-pyridine-3,4-diamine (44 mg, 0.32 mmol) in DCM (5 mL). The mixture is stirred at room temperature for 1 hour. A solution of thiazol-2-yl-2H-pyrazol-3-yl)-propionic acid ethyl ester in DCM (2 mL) is added. The mixture is reluxed for 3 days. On cooling, the reaction is quenched with water added dropwise and DCM (20 mL) is added. The organic layer is separated and the aqueous layer is extracted with DCM (3×30 mL). The combined organic layers are dried and the solvent removed. The residue is purified by PTLC (10% methanol in DCM) to give 1-ethyl-2-[1-(2-thiazol-2-yl-2H-pyrazol-3-yl)-ethyl]-1H-imidazo[4,5-c]pyridine as a solid. 1H NMR (CDCl3) δ 9.05 (s, 1H), 8.39 (d, 1H), 7.59 (d, 1H), 7.49 (d, 1H), 7.25 (d, 1H), 7.09 (d, 1H), 6.28 (d, 1H), 5.85 (q, 1H), 4.19 (q, 2H), 1.85 (d, 3H), 1.29 (t, 3H).
    • Example 39 Synthesis of 2-[2,4′]Bithiazolyl-5′-ylmethyl-1-ethyl-1H-imidazo[4,5-c]pyridine
  • Figure US20100267698A1-20101021-C00072
    • 1. Preparation of 4-Oxo-4-thiazol-2-yl-butyric acid ethyl ester
  • To a stirred solution of 2-trimethylsilanyl-thiazole (2.6 g, 16.53 mmol) in DCM (30 mL) is added a solution of 3-chlorocarbonyl-propionic acid ethyl ester (5.4 g, 32.81 mmol) in DCM (10 mL). The mixture is stirred overnight at room temperature. To the mixture is added 5% NaHCO3 (30 mL) and mixture is stirred at room temperature for 20 minutes. The organic layer is separated and the aqueous lay is extracted with DCM (2×50 mL). The combined organic layers are dried and the solvent removed. The residue is separated by column (methylene chloride) to give 4-oxo-4-thiazol-2-yl-butyric acid ethyl ester.
    • 2. Preparation of 3-Bromo-4-oxo-4-thiazol-2-yl-butyric acid ethyl ester
  • Phenyltrimethylammonium tribromide (3.4 g, 8.91 mmol) in DCM (20 mL) is added dropwise to a solution of 4-oxo-4-thiazol-2-yl-butyric acid ethyl ester (1.9 g, 8.91 mmol) in DCM (30 mL. The mixture is stirred overnight at room temperature. 5% NaHCO3 (40 mL) is added to the mixture. The organic layer is separated and the aqueous layer is extracted with DCM (2×40 mL). The combined organic layers are dried and solvent removed. The residue is purified by column (hexanes/ethyl acetate 3:1) to give 3-bromo-4-oxo-4-thiazol-2-yl-butyric acid ethyl ester.
    • 3. Preparation of [2,4′]Bithiazolyl-5′-yl-acetic acid ethyl ester
  • To a solution of 3-bromo-4-oxo-4-thiazol-2-yl-butyric acid ethyl ester (2.2 g, 7.53 mmol) in 1,4-dioxane (40 mL) is added thioformamide (freshly made from formamide and P2S5 in 1,4-dioxane) (22.59 mmol). The mixture is refluxed overnight. The solvent is removed and NaHCO3 (aq.) (40 mL) and DCM (100 mL) are added to the residue. The organic layer is separated and the aqueous layer is extracted with DCM (2×40 mL). The combined organic layers are dried and solvent removed. The crude is purified by column (1% methanol n DCM) to give [2,4′]bithiazolyl-5′-yl-acetic acid ethyl ester.
    • 4. Preparation of 2-[2,4′]Bithiazolyl-5′-ylmethyl-1-ethyl-1H-imidazo[4,5-c]pyridine
  • Trimethylaluminum (2M in toluene)(2.2 mL, 4.4 mmol) is added dropwise under N2 yo a solution of N4-Ethyl-pyridine-3,4-diamine (237 mg, 1.73 mmol) in DCM (5 mL). The mixture is stirred at room temperature for 1 hour. A solution of [2,4′]bithiazolyl-5′-yl-acetic acid ethyl ester (440 mg, 1.73 mmol) in DCM (2 mL) is added. The mixture is reluxed for 3 days. On cooling, the reaction is quenched with water added dropwise and DCM (40 mL) is added. The organic layer is separated and the aqueous layer is extracted with DCM (3×30 mL). The combined organic layers are dried and solvent removed. The residue is purified by PTLC (10% methanol in DCM) to give 2-[2,4′]bithiazolyl-5′-ylmethyl-1-ethyl-1H-imidazo[4,5-c]pyridine as a solid. 1H NMR (CDCl3) δ 9.05 (s, 1H0), 8.76 (s, 1H), 8.41 (d, 1H), 7.88 (d, 1H), 7.41 (d, 1H), 7.25 (d, 1H), 5.35 (s, 2H), 4.26 (q, 2H), 1.25 (t, 3H).
    • Example 40 Synthesis of 5-Bromo-1-ethyl-2-[3-(3-fluoro-phenyl)-isoxazol-4-ylmethyl]-1-benzoimidazole
  • Figure US20100267698A1-20101021-C00073
    • 1. Preparation of 3-(5-Bromo-1-ethyl-1H-benzoimidazol-2-yl)-1-(3-fluoro-phenyl)-propan-1-one
  • To a solution of 1-(3-fluoro-phenyl)-ethanone (0.55 g, 4.0 mmol) in THF (30 mL) at −78° C. under N2 is added LDA (2M in hexanes, 2.0 mL, 4.0 mmol). After 10 minutes, 5-bromo-2-chloromethyl-1-ethyl-1H-benzoimidazole (1 g, 3.65 mmol) in THF (5 mL) is added. The mixture is stirred at this temperature for an additional 1 hour and is gradually warmed to room temperature. The reaction is then quenched with saturated NH4Cl. The mixture is extracted with ethyl acetate (3×50 mL). Upon drying, the organic solvent is removed to give 3-(5-Bromo-1-ethyl-1H-benzoimidazol-2-yl)-1-(3-fluoro-phenyl)-propan-1-one as a yellow oil.
    • 2. Preparation of 5-Bromo-1-ethyl-2-[3-(3-fluoro-phenyl)-isoxazol-4-ylmethyl]-1H-benzoimidazole
  • A mixture of 3-(5-Bromo-1-ethyl-1H-benzoimidazol-2-yl)-1-(3-fluoro-phenyl)-propan-1-one (0.1 g, 0.27 mmol) and tris(dimethylamino)methane (0.077 g, 0.54 mmol) is heated at 60° C. in a sealed tube for 6 hours. The volatile material is removed in vacuo. To the residue is added EtOH (5 mL) and hydroxylamine (1.1 mmol). The mixture is heated at 120° C. for 2 hours. The solvent is removed. To the residue is added NaHCO3 (aq.) (10 mL) and DCM (30 mL). The organic layer is separated and the aqueous layer is extracted with DCM (2×15 mL). The combined organic layers are dried and the solvent removed. The crude is purified by PTLC (ethyl acetate/Hexanes 1:1) to give 5-Bromo-1-ethyl-2-[3-(3-fluoro-phenyl)-isoxazol-4-ylmethyl]-1H-benzoimidazole as a solid. 1H NMR (CDCl3) δ 8.40 (s, 1H), 7.88 (s, 1H), 7.31-7.52 (m, 4H), 7.18-7.22 (m, 2H), 4.14 (s, 2H), 4.04 (q, 2H), 1.18 (t, 3H).
    • Example 41 Synthesis of 5-Bromo-1-ethyl-2-[3-(3-fluoro-phenyl)-1H-pyrazol-4-ylmethyl]-1H-benzoimidazole
  • Figure US20100267698A1-20101021-C00074
  • A mixture of 3-(5-Bromo-1-ethyl-1H-benzoimidazol-2-yl)-1-(3-fluoro-phenyl)-propan-1-one (0.1 g, 0.27 mmol) and tris(dimethylamino)methane (0.077 g, 0.54 mmol) is heated at 60° C. in a sealed tube for 6 hours. The volatile material is removed in vacuo. EtOH (5 mL) and hydrazine acetate (1.1 mmol) are added to the residue. The mixture is heated at 120° C. for 2 hours. The solvent is removed. NaHCO3 (aq.) (10 mL) and DCM (30 mL) are added to the residue. The organic layer is separated and the aqueous layer is extracted with DCM (2×15 mL). The combined organic layers are dried and the solvent removed. The crude is purified by PTLC (ethyl acetate/Hexanes 1:1) to give 5-bromo-1-ethyl-2-[3-(3-fluoro-phenyl)-1H-pyrazol-4-ylmethyl]-1H-benzoimidazole as a solid. LRMS: calcd 399.3. found [M+1] 401.0.
    • Example 42 Synthesis of 5-Bromo-1-ethyl-2-[3-(3-fluoro-phenyl)-1-methyl-1H-pyrazol-4-ylmethyl]-1H-benzoimidazole and 5-Bromo-1-ethyl-2-[5-(3-fluoro-phenyl)-1-methyl-1H-pyrazol-4-ylmethyl]-1H-benzoimidazole
  • Figure US20100267698A1-20101021-C00075
  • A mixture of 3-(5-Bromo-1-ethyl-1H-benzoimidazol-2-yl)-1-(3-fluoro-phenyl)-propan-1-one (0.1 g, 0.27 mmol) and tris(dimethylamino)methane (0.077 g, 0.54 mmol) is heated at 60° C. in a sealed tube for 6 hours. The volatile material is removed in vacuo. EtOR (5 mL) and methyl hydrazine (1.1 mmol) are added to the residue. The mixture is heated at 120° C. for 2 hours. The solvent is removed. NaHCO3 (aq.) (10 mL) and DCM (30 mL) are added to the residue. The organic layer is separated and the aqueous layer is extracted with DCM (2×15 mL). The combined organic layers are dried and the solvent removed. The crude is purified by PTLC (ethyl acetate/Hexanes 1:1) to give 5-bromo-1-ethyl-2-[3-(3-fluoro-phenyl)-1-methyl-1H-pyrazol-4-ylmethyl]-1H-benzoimidazole and 5-bromo-1-ethyl-2-[5-(3-fluoro-phenyl)-1-methyl-1H-pyrazol-4-ylmethyl]-1H-benzoimidazole. 5-bromo-1-ethyl-2-[3-(3-fluoro-phenyl)-1-methyl-1H-pyrazol-4-ylmethyl]-1H-benzoimidazole: 1H NMR (CDCl3) δ 7.88 (s, 1H), 7.27-7.42 (m, 4H), 7.00-7.20 (m, 3H), 4.21 (s, 2H), 3.95 (t, 2H), 3.83 (s, 3H), 1.15 (t, 3H). 5-bromo-1-ethyl-2-[5-(3-fluoro-phenyl)-1-methyl-1H-pyrazol-4-ylmethyl]-1H-benzoimidazole: 1H NMR (CDCl3) δ 7.81 (s, 1H), 7.38-7.45 (m, 2H), 7.32 (dd, 1H), 7.00-7.18 (m, 4H), 4.00 (s, 2H), 3.90 (q, 2H), 3.78 (s, 3H), 1.15 (t, 3H).
    • Example 43 Synthesis of 5-Bromo-1-ethyl-2-[4-(3-fluoro-phenyl)-thiazol-5-ylmethyl]-1H-benzoimidazole
  • Figure US20100267698A1-20101021-C00076
  • A mixture of 3-(5-bromo-1-ethyl-1H-benzoimidazol-2-yl)-1-(3-fluoro-phenyl)-propan-1-one (0.2 g, 0.53 mmol) and bromine (0.1 g, 0.62 mmol) is refluxed in 1,4-dioxane for 2 hours. Thioformamide (freshly made from formamide and P2S5 in 1,4-dioxane) (1.3 mmol) are added to the solution. The mixture is refluxed overnight. The solvent is removed and NaHCO3 (aq.) (15 mL) and DCM (40 mL) are added to the residue. The organic layer is separated and the aqueous layer is extracted with DCM (2×30 mL). The combined organic layers are dried and solvent removed. The crude is purified by PTLC (5% methanol in DCM) to give 5-bromo-1-ethyl-2-[4-(3-fluoro-phenyl)-thiazol-5-ylmethyl]-1H-benzoimidazole. 1H NMR (CDCl3) δ 8.78 (s, 1H), 7.91 (s, 1H), 7.38-7.48 (m, 4H), 7.11-7.19 (m, 2H), 4.59 (s, 2H), 3.90 (q, 2H), 1.12 (t, 3H).
    • Example 44 Synthesis of 1-[3-Ethyl-2-(2-thiazol-2-yl-imidazol-1-ylmethyl)-3H-benzoimidazol-5-yl]-ethanone
  • Figure US20100267698A1-20101021-C00077
    • 1. Preparation of (5-Chloro-2-nitro-phenyl)-ethyl-amine
  • A mixture of 4-cloro-2-fluoronitrobenzene (5 g, 28.48 mmol), ethylamine (2M in THF, 24 mL, 48 mmol), and K2CO3 (7.9 g, 57 mmol) in acetonitrile (30 mL) is stirred at room temperature overnight. Solvent is removed. Ethyl acetate (80 mL) and brine (40 mL) are added to the residue. The organic layer is separated and the aqueous layer is extracted with ethyl acetate (2×50 mL). The combined organic layers are dried and solvent removed to give 5-chloro-2-nitro-phenyl)-ethyl-amine.
    • 2. Preparation of 1-(3-Ethylamino-4-nitro-phenyl)-ethanone
  • A mixture of 5-chloro-2-nitro-phenyl)-ethyl-amine (1 g, 4.98 mmol), tributyl-(1-ethoxyvinyl)stannane (2.7 g, 7.48 mmol) and dichlorobis(triphenylphosphine)palladium (II) (175 mg, 0.25 mmol) in toluene (80 mL) is heated at 100° C. in a sealed tube for 5 hours. On cooling, water (30 mL) and HCl (con. 30 mL) are added and the mixture is stirred at room temperature for 2 hours. The mixture is neutralized with NaOH (2 N) and extracted with DCM (3×50 ml). The combined organic layers are dried and solvent removed. The residue is purified by column (hexanes/ethyl acetate 3:1) to give 1-(3-ethylamino-4-nitro-phenyl)-ethanone.
    • 3. Preparation of 1-(4-Amino-3-ethylamino-phenyl)-ethanone
  • 10% Pd/C (100 mg) is added to a Parr bottle containing 1-(3-ethylamino-4-nitro-phenyl)-ethanone (0.98 g, 4.7 mmol) in ethanol (40 mL). The Parr bottle is sealed in a mechanical shaker, evacuated, and then purged with nitrogen followed by hydrogen. The system is pressurized to 50 PSI of hydrogen at room temperature and mechanical shaking engaged. After 2 hours, shaking is stopped, and the system purged with nitrogen prior to opening the vessel. The reaction mixture is filtered through celite, concentrated in vacuo, to give 1-(4-amino-3-ethylamino-phenyl)-ethanone.
    • 4. Preparation of 1-(2-Chloromethyl-3-ethyl-3H-benzoimidazol-5-yl)-ethanone
  • A mixture of 1-(4-amino-3-ethylamino-phenyl)-ethanone (300 mg, 1.69 mmol) and 2-chloroacetamidine hydrochloride (804 mg, 5.1 mmol) in acetonitrile (20 mL) is refluxed for 3 hours. Solvent is removed and the residue is treated with NaHCO3 (5%, 20 mL) and DCM (50 mL). The organic layer is separated and the aqueous layer is extracted with DCM (2×40 mL). The combined organic layers are dried and solvent removed to give 1-(2-Chloromethyl-3-ethyl-3H-benzoimidazol-5-yl)-ethanone.
    • 5. Preparation of 1-[3-Ethyl-2-(2-thiazol-2-yl-imidazol-1-ylmethyl)-3H-benzoimidazol-5-yl]-ethanone
  • A mixture of 1-(2-Chloromethyl-3-ethyl-3H-benzoimidazol-5-yl)-ethanone (150 mg, 0.63 mmol), 2-(1H-Imidazol-2-yl)-thiazole (95 mg, 0.63 mmol) and K2CO3 (262 mg, 1.9 mmol) in DMF (10 mL) is stirred at room temperature for 48 hours. Brine (6 mL) and DCM (20 mL) are added. The organic layer is separated and the aqueous layer is extracted with DCM (3×29 mL). The combined organic layers are dried and solvent removed. The residue is purified by PTLC (10% methanol in DCM) to give 1-[3-Ethyl-2-(2-thiazol-2-yl-imidazol-1-ylmethyl)-3H-benzoimidazol-5-yl]-ethanone as a solid. 1H NMR (CDCl3) δ 8.05 (d, 1H), 7.92 (dd, 1H), 7.87 (dd, 1H), 7.81 (dd, 1H), 7.40 (d, 1H), 7.20 (d, 1H), 7.14 (d, 1H), 6.17 (s, 2H), 4.3.3 (q, 2H), 2.67 (s, 3H), 1.13 (t, 3H).
    • Example 45 Synthesis of 1-Ethyl-2-[(2-(1H-pyrazol-3-yl)-imidazol-1-ylmethyl]-1H-benzoimidazole-5-carbonitrile
  • Figure US20100267698A1-20101021-C00078
    • 1. Preparation of 3-(H-Imidazol-2-yl)-pyrazole-1-carboxylic acid tert-butyl ester
  • A mixture of 1H-Pyrazole-3-carbaldehyde (1 g, 10.4 mmol) di-tert-butyl dicarbonate (11.4 mmol) and N,N-dimethylpyridine (20 mg) in DCM (15 mL) and methanol (5 mL) is stirred at room temperature overnight. Solvent is removed. Glyoxal (6 mL) and ammonium hydroxid (8 mL) are added to the residue and the mixture is stirred at room temperature for 1.5 hours. Acetic acid is added dropwise to the mixture to pH ˜7. Solvent is removed and DCM (50 mL) and brine (30 mL) are added to the residue. The organic layer is separated and the aqueous layer is extracted with DCM (2×40 mL). The combined organic layers are dried and solvent removed to give 3-(1H-imidazol-2-yl)-pyrazole-1-carboxylic acid tert-butyl ester.
    • 2. Preparation of 1-Ethyl-2-[2-(1H-pyrazol-3-yl)-imidazol-1-ylmethyl]-1H-benzoimidazole-5-carbonitrile
  • As described previously, nucleophilic displacement of 2-Chloromethyl-1-ethyl-1H-benzoimidazole-5-carbonitrile with 3-(1H-Imidazol-2-yl)-pyrazole-1-carboxylic acid tert-butyl ester followed by usual work-up provides 1-Ethyl-2-[2-(1H-pyrazol-3-yl)-imidazol-1-ylmethyl]-1H-benzoimidazole-5-carbonitrile; 1H NMR (CD3OD) δ 7.97 (s, 1H), 7.53 (brs, 1H), 7.46 (d, J=8.7 Hz, 1H), 7.35 (d, J=8.1 Hz, 1H), 6.97 (d, J=6.3 Hz, 2H), 6.79 (br s, 1H), 6.10 (, 2H), 4.22 (q, J=7.2 Hz, 2H), 0.88 (t, J=7.2 Hz, 3H); m/z 318 [M+1].
    • Example 46 Synthesis of 4-(1-ethyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-1H-benzimidazol-5-yl)-2-methylbutan-2-ol
  • Figure US20100267698A1-20101021-C00079
    • 1. Preparation of 4-(1-ethyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-1H-benzimidazol-5-yl)-2-methyl-1-but-3-yn-2-ol
  • Pd (PPh3)4 (15 mg, 0.013 mmol), CuI (5 mg, 0.026 mmol), and 2-methyl-but-3-yn-2-ol (250 μL, 2.6 mmol) are added to the solution of 1-ethyl-5-bromo-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-1H-benzimidazole (100 mg, 0.26 mmol) in i-Pr2NH (5 mL). The resulting mixture is heated to 100° C. for 20 hr, and then diluted with water. The aqueous layer is extracted with ethyl acetate. The combined organic layers are dried over Na2SO4 and solvent is removed to give a brown oil. Purification by preparative TLC provides 40 mg of product as yellow solid. LCMS 392.37 (MH+).
    • 2. Preparation of 4-(1-ethyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-1H-benzimidazol-5-yl)-2-methylbutan-2-ol
  • To a Parr bottle containing 4-(1-ethyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-1H-benzimidazol-5-yl)-2-methyl-1-but-3-yn-2-ol (40 mg, 0.1 mmol) in ethanol (10 mL) is added 10% Pd/C (50 mg). The Parr bottle is sealed in a mechanical shaker, evacuated, and then purged with nitrogen followed by hydrogen. The system is pressurized to 40 PSI of hydrogen at room temperature and mechanical shaking engaged. After 2 hours, shaking is stopped, and the system purged with nitrogen prior to opening the vessel. The reaction mixture is filtered through celite and concentrated in vacuo. The product is obtained as white solid. 1H NMR (CDCl3) δ 7.85 (d, 1H), 7.60 (s, 1H), 7.38 (d, 1H), 7.22 (m, 1H) 7.15 (m, 2H), 7.09 (s, 1H), 6.31 (s, 2H), 4.17 (q, 2H), 2.80 (m, 2H), 1.81 (m, 2H), 1.22 (d, 6H), 1.01 (t, 3H). LCMS 396.38 (MH+).
    • Example 47 Synthesis of 1-(1-ethyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1yl]methyl}-1H-benzimidazol-5-yl)-4-hydroxypentan-1-one
  • Figure US20100267698A1-20101021-C00080
  • To the bi-layer system of 3 N HCl (5 mL) and CH2Cl2 is added 5-(1-ethyl-2-{[2-(1,3-thiazol-2-yl)-1H-imidazol-1-yl]methyl}-1H-benzimidazol-5-yl)-pent-4-yn-2-ol (107 mg, 0.27 mmol), PdCl2 (5 mg, 0.028 mmol), and n-Bu4NCl (17 mg, 0.056 mmol). The resulting mixture is stirred at room temperature for 72 hr, and then poured into saturated NaHCO3 solution. The aqueous layer is extracted with CH2Cl2 and the combined organic layers are dried over Na2SO4. Solvent is removed and purification by preparative TLC provides the product as white solid. 1H NMR (CDCl3) δ 8.44 (d, 1H), 7.80 (dd, 1H), 7.85 (d, 1H), 7.40 (d, 1H), 7.36 (d, 1H), 7.18 (d, 1H), 7.14 (d, 1H), 6.36 (s, 2H), 4.27 (q, 2H), 3.86 (m, 1H), 3.19 (t, 2H), 1.93 (m, 2H), 1.25 (d, 3H), 1.09 (t, 3H). LCMS 410.2 (MH+).
    • Example 48 Synthesis of 8-{[2-(6-Fluoropyridin-2-yl)-1H-imidazol-1-yl]methyl}-2,9-dimethyl-9H-purine
  • Figure US20100267698A1-20101021-C00081
    • 1) Preparation of 6-Amino-4-chloro-2,N-dimethyl-5-nitropyrimidine
  • A solution of Methylamine (15.4 mL 1M in THF, diluted with 10 mL of hexane) is added dropwise to a solution of 4,6-Dichloro-2-methyl-5-nitropyrimidine (3.2 g, 15.4 mmol) in Hexane (30 mL). After addition, the reaction mixture is stirred at room temperature for one hour, and concentrated in vacuo to a solid. After the solid is dissolved in 50 mL of CH2Cl2, the resultant solution is washed with 0.1 N HCl (20 mL) and water (20 mL), dried over Na2SO4 and concentrated to a yellow solid. 1H NMR (CDCl3): δ 7.7 (bs, 1H), 3.10 (d, 3H), 2.55 (s, 5H).
    • 2) Preparation of 2,N-Dimethyl-4,5-diaminopyrimidine
  • A mixture of 6-Amino-4-chloro-2,N-dimethyl-5-nitropyrimidine (1.0 g, 0.5 mmol), NaOH (40 mg, 1 mmol) and 1.0 g 10% Pd/C in 50 mL of 2% aqueous THF is hydrogenated at 50 PSI of hydrogen at room temperature overnight. The reaction mixture is filtered through celite, concentrated in vacuo, and the obtained solid is purified on a silica gel column eluting with 10/1/0.1 CH2Cl2/MeOH/NH4OH to give the title compound. 1H NMR (CDCl3) δ 7.64 (s, 1H), 4.95 (bs, 1H), 3.02 (d, 3H), 2.45 (s, 3H), 1.90 (bs, 2H).
    • 3) Preparation of 8-chloromethyl-2,9-dimethyl-9H-purine
  • A solution of 2,N-Dimethyl-4,5-diaminopyrimidine (180 mg, 1.3 mmol) and ethyl chloroacetimidate hydrochloride (310 g, 2.0 mmol) in dichloroethane (10 mL) is heated at reflux for 17 h and cooled. The mixture is washed with aqueous NaHCO3, water, dried and concentrated to give 8-Chloromethyl-2,9-dimethyl-9H-purine.
    • 4) Preparation 8-{[2-(6-fluoropyridin-2-yl)-1H-imidazol-1-yl]methyl}-2,9-dimethyl-9H-purine
  • A mixture of 8-Chloromethyl-2,9-dimethyl-9H-purine (40 mg, 0.2 mmol), 2-Fluoro-6-(1H-imidazol-2-yl)-pyridine (40 mg, 0.25 mmol) and K2CO3 (55 mg, 0.4 mmol) in 2 mL of DMF is stirred at room temperature for four hours. The mixture is diluted with water (10 mL), and extracted with ethyl acetate three times. The combined extract is washed with brine, dried and concentrated. The residue is purified by preparative thin layer chromatography to give the title compound. 1H NMR (CDCl3): δ 8.92 (s, 1H), 8.18 (dd, 1H), 7.88 (q, 1H), 7.25 (s, 1H), 7.20 (s, 1H), 7.11 (s, 1H), 6.83 (dd, 1H), 6.22 (s, 2H), 3.89 (s, 3H), 2.8 (s, 3H). LRMS calcd 323. found 324 (MH+).
    • Example 49
  • The following compounds are prepared essentially according to the procedures in the previous examples.
    • a) 3-Methyl-2-(2-oxazol-2-yl-imidazol-1-ylmethyl)-3H-imidazo[4,5-c]pyridine
    • b) 3-Ethyl-2-(2-[1,3,4]oxadiazol-2-yl-imidazol-1-ylmethyl)-3H-imidazo[4,5-c]pyridine
    • c) 3-Ethyl-2-[2-(3-methyl-pyridin-2-yl)-imidazol-1-ylmethyl]-3H-imidazo[4,5-c]pyridine
    Example 50
  • The compounds listed in tables 1-8 are prepared essentially according to the procedures set forth above in Schemes I-X and the preceding examples.
  • In tables 1-5 the designations X1, X2, W1, X5, W6, etc., on the substituents WI, R5, etc., indicate the point of attachment of the substituent to the parent structural formula. For example, R5 in compound number (hereinafter “Cmp. #”) 112 is an ethyl group; R5 in compound 134 is a cyclopropylmethyl group; and W1 in compound 132 is a 3-chlorophenyl group.
  • LC-MS data is provided for a number of the compounds in tables 1-5. The following HPLC method was used to obtain this data: YMC-pack pro C18 column, 33×4.6 mm(L×ID) 5 μm particle size. 3 min gradient from 5% to 95% B with 0.5 min hold at 95% B. Solvent A: 95% H2O-5% MeOH-0.05% TFA; Solvent B: 95% MeOH-5% H2O-0.05% TFA). Flow rate=2.0 ml/min. Injection volume=1 μl. MS (ES+) m/e 360 [MH]+. The LC data is given as HPLC retention times.
  • TABLE 1
    Figure US20100267698A1-20101021-C00082
    GC/FID
    Cmp. # R5 W1 MW RT Mass or LC/MS
    100
    Figure US20100267698A1-20101021-C00083
    Figure US20100267698A1-20101021-C00084
         		302.3792 1.60 303.1 LC/MS
    101
    Figure US20100267698A1-20101021-C00085
    Figure US20100267698A1-20101021-C00086
         		316.406 1.80 317.1 LC/MS
    102
    Figure US20100267698A1-20101021-C00087
    Figure US20100267698A1-20101021-C00088
         		316.406 1.80 317.1 LC/MS
    103
    Figure US20100267698A1-20101021-C00089
    Figure US20100267698A1-20101021-C00090
         		320.3693 5.06 GC/FID
    104
    Figure US20100267698A1-20101021-C00091
    Figure US20100267698A1-20101021-C00092
         		334.3961 5.07 GC/FID
    105
    Figure US20100267698A1-20101021-C00093
    Figure US20100267698A1-20101021-C00094
         		334.3961 5.17 GC/FID
    106
    Figure US20100267698A1-20101021-C00095
    Figure US20100267698A1-20101021-C00096
         		306.3425 5.12 GC/FID
    107
    Figure US20100267698A1-20101021-C00097
    Figure US20100267698A1-20101021-C00098
         		320.3693 5.11 GC/FID
    108
    Figure US20100267698A1-20101021-C00099
    Figure US20100267698A1-20101021-C00100
         		334.3961 5.10 GC/FID
    109
    Figure US20100267698A1-20101021-C00101
    Figure US20100267698A1-20101021-C00102
         		334.3961 5.20 GC/FID
    110
    Figure US20100267698A1-20101021-C00103
    Figure US20100267698A1-20101021-C00104
         		334.3961 5.21 GC/FID
    111
    Figure US20100267698A1-20101021-C00105
    Figure US20100267698A1-20101021-C00106
         		324.3326 4.95 GC/FID
    112
    Figure US20100267698A1-20101021-C00107
    Figure US20100267698A1-20101021-C00108
         		338.3594 4.95 GC/FID
    113
    Figure US20100267698A1-20101021-C00109
    Figure US20100267698A1-20101021-C00110
         		352.3862 4.95 GC/FID
    114
    Figure US20100267698A1-20101021-C00111
    Figure US20100267698A1-20101021-C00112
         		352.3862 5.05 GC/FID
    115
    Figure US20100267698A1-20101021-C00113
    Figure US20100267698A1-20101021-C00114
         		354.8144 5.43 GC/FID
    116
    Figure US20100267698A1-20101021-C00115
    Figure US20100267698A1-20101021-C00116
         		368.8412 5.42 GC/FID
    117
    Figure US20100267698A1-20101021-C00117
    Figure US20100267698A1-20101021-C00118
         		368.8412 5.51 GC/FID
    118
    Figure US20100267698A1-20101021-C00119
    Figure US20100267698A1-20101021-C00120
         		308.4074 5.27 GC/FID
    119
    Figure US20100267698A1-20101021-C00121
    Figure US20100267698A1-20101021-C00122
         		322.4342 5.26 GC/FID
    120
    Figure US20100267698A1-20101021-C00123
    Figure US20100267698A1-20101021-C00124
         		322.4342 5.37 GC/FID
    121
    Figure US20100267698A1-20101021-C00125
    Figure US20100267698A1-20101021-C00126
         		356.3495 4.99 GC/FID
    122
    Figure US20100267698A1-20101021-C00127
    Figure US20100267698A1-20101021-C00128
         		370.3763 4.98 GC/FID
    123
    Figure US20100267698A1-20101021-C00129
    Figure US20100267698A1-20101021-C00130
         		384.4031 4.98 GC/FID
    124
    Figure US20100267698A1-20101021-C00131
    Figure US20100267698A1-20101021-C00132
         		384.4031 5.07 GC/FID
    125
    Figure US20100267698A1-20101021-C00133
    Figure US20100267698A1-20101021-C00134
         		367.2485 5.68 GC/FID
    126
    Figure US20100267698A1-20101021-C00135
    Figure US20100267698A1-20101021-C00136
         		381.2753 5.67 GC/FID
    127
    Figure US20100267698A1-20101021-C00137
    Figure US20100267698A1-20101021-C00138
         		395.3021 5.65 GC/FID
    128
    Figure US20100267698A1-20101021-C00139
    Figure US20100267698A1-20101021-C00140
         		395.3021 5.74 GC/FID
    129
    Figure US20100267698A1-20101021-C00141
    Figure US20100267698A1-20101021-C00142
         		322.7975 1.67 323.1 LC/MS
    130
    Figure US20100267698A1-20101021-C00143
    Figure US20100267698A1-20101021-C00144
         		336.8243 1.87 337.3 LC/MS
    131
    Figure US20100267698A1-20101021-C00145
    Figure US20100267698A1-20101021-C00146
         		350.8511 2.00 351.1 LC/MS
    132
    Figure US20100267698A1-20101021-C00147
    Figure US20100267698A1-20101021-C00148
         		350.8511 2.07 351.3 LC/MS
    133
    Figure US20100267698A1-20101021-C00149
    Figure US20100267698A1-20101021-C00150
         		330.4328 2.00 331.3 LC/MS
    134
    Figure US20100267698A1-20101021-C00151
    Figure US20100267698A1-20101021-C00152
         		328.417 1.87 329.2 LC/MS
    135
    Figure US20100267698A1-20101021-C00153
    Figure US20100267698A1-20101021-C00154
         		348.4229 5.15 GC/FID
    136
    Figure US20100267698A1-20101021-C00155
    Figure US20100267698A1-20101021-C00156
         		346.4071 5.28 GC/FID
    137
    Figure US20100267698A1-20101021-C00157
    Figure US20100267698A1-20101021-C00158
         		348.4229 5.18 GC/FID
    138
    Figure US20100267698A1-20101021-C00159
    Figure US20100267698A1-20101021-C00160
         		362.4497 5.32 GC/FID
    139
    Figure US20100267698A1-20101021-C00161
    Figure US20100267698A1-20101021-C00162
         		346.4071 5.31 GC/FID
    140
    Figure US20100267698A1-20101021-C00163
    Figure US20100267698A1-20101021-C00164
         		346.4071 5.31 GC/FID
    141
    Figure US20100267698A1-20101021-C00165
    Figure US20100267698A1-20101021-C00166
         		366.413 5.04 GC/FID
    142
    Figure US20100267698A1-20101021-C00167
    Figure US20100267698A1-20101021-C00168
         		380.4398 5.18 GC/FID
    143
    Figure US20100267698A1-20101021-C00169
    Figure US20100267698A1-20101021-C00170
         		364.3972 5.16 GC/FID
    144
    Figure US20100267698A1-20101021-C00171
    Figure US20100267698A1-20101021-C00172
         		382.868 5.47 GC/FID
    145
    Figure US20100267698A1-20101021-C00173
    Figure US20100267698A1-20101021-C00174
         		380.8522 5.59 GC/FID
    146
    Figure US20100267698A1-20101021-C00175
    Figure US20100267698A1-20101021-C00176
         		336.461 5.33 GC/FID
    147
    Figure US20100267698A1-20101021-C00177
    Figure US20100267698A1-20101021-C00178
         		334.4452 5.46 GC/FID
    148
    Figure US20100267698A1-20101021-C00179
    Figure US20100267698A1-20101021-C00180
         		398.4299 5.04 GC/FID
    149
    Figure US20100267698A1-20101021-C00181
    Figure US20100267698A1-20101021-C00182
         		396.4141 5.17 GC/FID
    150
    Figure US20100267698A1-20101021-C00183
    Figure US20100267698A1-20101021-C00184
         		409.3289 5.68 GC/FID
    151
    Figure US20100267698A1-20101021-C00185
    Figure US20100267698A1-20101021-C00186
         		423.3557 5.80 GC/FID
    152
    Figure US20100267698A1-20101021-C00187
    Figure US20100267698A1-20101021-C00188
         		407.3131 5.81 GC/FID
    153
    Figure US20100267698A1-20101021-C00189
    Figure US20100267698A1-20101021-C00190
         		364.8779 2.20 365.2 LC/MS
    154
    Figure US20100267698A1-20101021-C00191
    Figure US20100267698A1-20101021-C00192
         		378.9047 2.40 379.3 LC/MS
    155
    Figure US20100267698A1-20101021-C00193
    Figure US20100267698A1-20101021-C00194
         		362.8621 2.07 363.3 LC/MS
  • TABLE 2
    Figure US20100267698A1-20101021-C00195
    Cmp. GC/FID or
    # R2 R5 W6 RT LC/MS
    156
    Figure US20100267698A1-20101021-C00196
    Figure US20100267698A1-20101021-C00197
    Figure US20100267698A1-20101021-C00198
         		9.18 GC/FID
    157
    Figure US20100267698A1-20101021-C00199
    Figure US20100267698A1-20101021-C00200
    Figure US20100267698A1-20101021-C00201
         		8.67 GC/FID
    158
    Figure US20100267698A1-20101021-C00202
    Figure US20100267698A1-20101021-C00203
    Figure US20100267698A1-20101021-C00204
         		10.56 GC/FID
    159
    Figure US20100267698A1-20101021-C00205
    Figure US20100267698A1-20101021-C00206
    Figure US20100267698A1-20101021-C00207
         		9.39 GC/FID
    160
    Figure US20100267698A1-20101021-C00208
    Figure US20100267698A1-20101021-C00209
    Figure US20100267698A1-20101021-C00210
         		8.87 GC/FID
    161
    Figure US20100267698A1-20101021-C00211
    Figure US20100267698A1-20101021-C00212
    Figure US20100267698A1-20101021-C00213
         		10.91 GC/FID
    162
    Figure US20100267698A1-20101021-C00214
    Figure US20100267698A1-20101021-C00215
    Figure US20100267698A1-20101021-C00216
         		9.12 GC/FID
    163
    Figure US20100267698A1-20101021-C00217
    Figure US20100267698A1-20101021-C00218
    Figure US20100267698A1-20101021-C00219
         		8.61 LC/MS
    164
    Figure US20100267698A1-20101021-C00220
    Figure US20100267698A1-20101021-C00221
    Figure US20100267698A1-20101021-C00222
         		10.46 GC/FID
    165
    Figure US20100267698A1-20101021-C00223
    Figure US20100267698A1-20101021-C00224
    Figure US20100267698A1-20101021-C00225
         		8.98 GC/FID
    166
    Figure US20100267698A1-20101021-C00226
    Figure US20100267698A1-20101021-C00227
    Figure US20100267698A1-20101021-C00228
         		8.49 GC/FID
    167
    Figure US20100267698A1-20101021-C00229
    Figure US20100267698A1-20101021-C00230
    Figure US20100267698A1-20101021-C00231
         		10.28 GC/FID
    168
    Figure US20100267698A1-20101021-C00232
    Figure US20100267698A1-20101021-C00233
    Figure US20100267698A1-20101021-C00234
         		9.77 GC/FID
    169
    Figure US20100267698A1-20101021-C00235
    Figure US20100267698A1-20101021-C00236
    Figure US20100267698A1-20101021-C00237
         		9.17 GC/FID
    170
    Figure US20100267698A1-20101021-C00238
    Figure US20100267698A1-20101021-C00239
    Figure US20100267698A1-20101021-C00240
         		11.41 LC/MS
    171
    Figure US20100267698A1-20101021-C00241
    Figure US20100267698A1-20101021-C00242
    Figure US20100267698A1-20101021-C00243
         		8.75 GC/FID
    172
    Figure US20100267698A1-20101021-C00244
    Figure US20100267698A1-20101021-C00245
    Figure US20100267698A1-20101021-C00246
         		8.31 GC/FID
    173
    Figure US20100267698A1-20101021-C00247
    Figure US20100267698A1-20101021-C00248
    Figure US20100267698A1-20101021-C00249
         		9.97 GC/FID
    174
    Figure US20100267698A1-20101021-C00250
    Figure US20100267698A1-20101021-C00251
    Figure US20100267698A1-20101021-C00252
         		9.04 GC/FID
    175
    Figure US20100267698A1-20101021-C00253
    Figure US20100267698A1-20101021-C00254
    Figure US20100267698A1-20101021-C00255
         		8.55 GC/FID
    176
    Figure US20100267698A1-20101021-C00256
    Figure US20100267698A1-20101021-C00257
    Figure US20100267698A1-20101021-C00258
         		10.36 GC/FID
    177
    Figure US20100267698A1-20101021-C00259
    Figure US20100267698A1-20101021-C00260
    Figure US20100267698A1-20101021-C00261
         		9.81 GC/FID
    178
    Figure US20100267698A1-20101021-C00262
    Figure US20100267698A1-20101021-C00263
    Figure US20100267698A1-20101021-C00264
         		9.21 GC/FID
    179
    Figure US20100267698A1-20101021-C00265
    Figure US20100267698A1-20101021-C00266
    Figure US20100267698A1-20101021-C00267
         		11.49 GC/FID
    180
    Figure US20100267698A1-20101021-C00268
    Figure US20100267698A1-20101021-C00269
    Figure US20100267698A1-20101021-C00270
         		9.64 GC/FID
    181
    Figure US20100267698A1-20101021-C00271
    Figure US20100267698A1-20101021-C00272
    Figure US20100267698A1-20101021-C00273
         		9.05 GC/FID
    182
    Figure US20100267698A1-20101021-C00274
    Figure US20100267698A1-20101021-C00275
    Figure US20100267698A1-20101021-C00276
         		11.22 LC/MS
    183
    Figure US20100267698A1-20101021-C00277
    Figure US20100267698A1-20101021-C00278
    Figure US20100267698A1-20101021-C00279
         		10.86 GC/FID
    184
    Figure US20100267698A1-20101021-C00280
    Figure US20100267698A1-20101021-C00281
    Figure US20100267698A1-20101021-C00282
         		10.08 GC/FID
    185
    Figure US20100267698A1-20101021-C00283
    Figure US20100267698A1-20101021-C00284
    Figure US20100267698A1-20101021-C00285
         		12.97 GC/FID
    186
    Figure US20100267698A1-20101021-C00286
    Figure US20100267698A1-20101021-C00287
    Figure US20100267698A1-20101021-C00288
         		9.85 GC/FID
    187
    Figure US20100267698A1-20101021-C00289
    Figure US20100267698A1-20101021-C00290
    Figure US20100267698A1-20101021-C00291
         		9.23 GC/FID
    188
    Figure US20100267698A1-20101021-C00292
    Figure US20100267698A1-20101021-C00293
    Figure US20100267698A1-20101021-C00294
         		11.52 GC/FID
  • TABLE 3
    Figure US20100267698A1-20101021-C00295
    GC/FID or
    Cpd. # R2 R5 W1 RT LC/MS
    189
    Figure US20100267698A1-20101021-C00296
    Figure US20100267698A1-20101021-C00297
    Figure US20100267698A1-20101021-C00298
         		6.17 GC/FID
    190
    Figure US20100267698A1-20101021-C00299
    Figure US20100267698A1-20101021-C00300
    Figure US20100267698A1-20101021-C00301
         		6.02 GC/FID
    191
    Figure US20100267698A1-20101021-C00302
    Figure US20100267698A1-20101021-C00303
    Figure US20100267698A1-20101021-C00304
         		6.32 GC/FID
    192
    Figure US20100267698A1-20101021-C00305
    Figure US20100267698A1-20101021-C00306
    Figure US20100267698A1-20101021-C00307
         		6.15 GC/FID
    193
    Figure US20100267698A1-20101021-C00308
    Figure US20100267698A1-20101021-C00309
    Figure US20100267698A1-20101021-C00310
         		6.37 GC/FID
    194
    Figure US20100267698A1-20101021-C00311
    Figure US20100267698A1-20101021-C00312
    Figure US20100267698A1-20101021-C00313
         		6.20 GC/FID
    195
    Figure US20100267698A1-20101021-C00314
    Figure US20100267698A1-20101021-C00315
    Figure US20100267698A1-20101021-C00316
         		6.40 GC/FID
    196
    Figure US20100267698A1-20101021-C00317
    Figure US20100267698A1-20101021-C00318
    Figure US20100267698A1-20101021-C00319
         		1.32 LC/MS
    197
    Figure US20100267698A1-20101021-C00320
    Figure US20100267698A1-20101021-C00321
    Figure US20100267698A1-20101021-C00322
         		6.52 GC/FID
    198
    Figure US20100267698A1-20101021-C00323
    Figure US20100267698A1-20101021-C00324
    Figure US20100267698A1-20101021-C00325
         		6.36 GC/FID
    199
    Figure US20100267698A1-20101021-C00326
    Figure US20100267698A1-20101021-C00327
    Figure US20100267698A1-20101021-C00328
         		6.77 GC/FID
    200
    Figure US20100267698A1-20101021-C00329
    Figure US20100267698A1-20101021-C00330
    Figure US20100267698A1-20101021-C00331
         		6.60 GC/FID
    201
    Figure US20100267698A1-20101021-C00332
    Figure US20100267698A1-20101021-C00333
    Figure US20100267698A1-20101021-C00334
         		6.37 GC/FID
    202
    Figure US20100267698A1-20101021-C00335
    Figure US20100267698A1-20101021-C00336
    Figure US20100267698A1-20101021-C00337
         		6.22 GC/FID
    203
    Figure US20100267698A1-20101021-C00338
    Figure US20100267698A1-20101021-C00339
    Figure US20100267698A1-20101021-C00340
         		1.40 LC/MS
    204
    Figure US20100267698A1-20101021-C00341
    Figure US20100267698A1-20101021-C00342
    Figure US20100267698A1-20101021-C00343
         		6.50 GC/FID
    205
    Figure US20100267698A1-20101021-C00344
    Figure US20100267698A1-20101021-C00345
    Figure US20100267698A1-20101021-C00346
         		6.66 GC/FID
    206
    Figure US20100267698A1-20101021-C00347
    Figure US20100267698A1-20101021-C00348
    Figure US20100267698A1-20101021-C00349
         		6.51 GC/FID
    207
    Figure US20100267698A1-20101021-C00350
    Figure US20100267698A1-20101021-C00351
    Figure US20100267698A1-20101021-C00352
         		7.15 GC/FID
    208
    Figure US20100267698A1-20101021-C00353
    Figure US20100267698A1-20101021-C00354
    Figure US20100267698A1-20101021-C00355
         		6.91 GC/FID
    209
    Figure US20100267698A1-20101021-C00356
    Figure US20100267698A1-20101021-C00357
    Figure US20100267698A1-20101021-C00358
         		7.13 GC/FID
    210
    Figure US20100267698A1-20101021-C00359
    Figure US20100267698A1-20101021-C00360
    Figure US20100267698A1-20101021-C00361
         		6.91 GC/FID
    211
    Figure US20100267698A1-20101021-C00362
    Figure US20100267698A1-20101021-C00363
    Figure US20100267698A1-20101021-C00364
         		5.64 GC/FID
    212
    Figure US20100267698A1-20101021-C00365
    Figure US20100267698A1-20101021-C00366
    Figure US20100267698A1-20101021-C00367
         		5.47 GC/FID
    213
    Figure US20100267698A1-20101021-C00368
    Figure US20100267698A1-20101021-C00369
    Figure US20100267698A1-20101021-C00370
         		5.92 GC/FID
    214
    Figure US20100267698A1-20101021-C00371
    Figure US20100267698A1-20101021-C00372
    Figure US20100267698A1-20101021-C00373
         		5.75 GC/FID
    215
    Figure US20100267698A1-20101021-C00374
    Figure US20100267698A1-20101021-C00375
    Figure US20100267698A1-20101021-C00376
         		1.13 LC/MS
    216
    Figure US20100267698A1-20101021-C00377
    Figure US20100267698A1-20101021-C00378
    Figure US20100267698A1-20101021-C00379
         		5.71 GC/FID
    217
    Figure US20100267698A1-20101021-C00380
    Figure US20100267698A1-20101021-C00381
    Figure US20100267698A1-20101021-C00382
         		5.91 GC/FID
    218
    Figure US20100267698A1-20101021-C00383
    Figure US20100267698A1-20101021-C00384
    Figure US20100267698A1-20101021-C00385
         		5.74 GC/FID
    219
    Figure US20100267698A1-20101021-C00386
    Figure US20100267698A1-20101021-C00387
    Figure US20100267698A1-20101021-C00388
         		6.05 GC/FID
    220
    Figure US20100267698A1-20101021-C00389
    Figure US20100267698A1-20101021-C00390
    Figure US20100267698A1-20101021-C00391
         		5.89 GC/FID
    221
    Figure US20100267698A1-20101021-C00392
    Figure US20100267698A1-20101021-C00393
    Figure US20100267698A1-20101021-C00394
         		5.99 GC/FID
    222
    Figure US20100267698A1-20101021-C00395
    Figure US20100267698A1-20101021-C00396
    Figure US20100267698A1-20101021-C00397
         		5.82 GC/FID
    223
    Figure US20100267698A1-20101021-C00398
    Figure US20100267698A1-20101021-C00399
    Figure US20100267698A1-20101021-C00400
         		6.11 GC/FID
    224
    Figure US20100267698A1-20101021-C00401
    Figure US20100267698A1-20101021-C00402
    Figure US20100267698A1-20101021-C00403
         		5.96 GC/FID
    225
    Figure US20100267698A1-20101021-C00404
    Figure US20100267698A1-20101021-C00405
    Figure US20100267698A1-20101021-C00406
         		6.09 GC/FID
    226
    Figure US20100267698A1-20101021-C00407
    Figure US20100267698A1-20101021-C00408
    Figure US20100267698A1-20101021-C00409
         		5.94 GC/FID
    227
    Figure US20100267698A1-20101021-C00410
    Figure US20100267698A1-20101021-C00411
    Figure US20100267698A1-20101021-C00412
         		6.11 GC/FID
    228
    Figure US20100267698A1-20101021-C00413
    Figure US20100267698A1-20101021-C00414
    Figure US20100267698A1-20101021-C00415
         		5.96 GC/FID
    229
    Figure US20100267698A1-20101021-C00416
    Figure US20100267698A1-20101021-C00417
    Figure US20100267698A1-20101021-C00418
         		6.33 GC/FID
    230
    Figure US20100267698A1-20101021-C00419
    Figure US20100267698A1-20101021-C00420
    Figure US20100267698A1-20101021-C00421
         		6.18 GC/FID
    231
    Figure US20100267698A1-20101021-C00422
    Figure US20100267698A1-20101021-C00423
    Figure US20100267698A1-20101021-C00424
         		6.33 GC/FID
    232
    Figure US20100267698A1-20101021-C00425
    Figure US20100267698A1-20101021-C00426
    Figure US20100267698A1-20101021-C00427
         		6.19 GC/FID
    233
    Figure US20100267698A1-20101021-C00428
    Figure US20100267698A1-20101021-C00429
    Figure US20100267698A1-20101021-C00430
         		6.21 GC/FID
    234
    Figure US20100267698A1-20101021-C00431
    Figure US20100267698A1-20101021-C00432
    Figure US20100267698A1-20101021-C00433
         		6.07 GC/FID
    235
    Figure US20100267698A1-20101021-C00434
    Figure US20100267698A1-20101021-C00435
    Figure US20100267698A1-20101021-C00436
         		5.81 GC/FID
    236
    Figure US20100267698A1-20101021-C00437
    Figure US20100267698A1-20101021-C00438
    Figure US20100267698A1-20101021-C00439
         		5.64 GC/FID
    237
    Figure US20100267698A1-20101021-C00440
    Figure US20100267698A1-20101021-C00441
    Figure US20100267698A1-20101021-C00442
         		6.29 GC/FID
    238
    Figure US20100267698A1-20101021-C00443
    Figure US20100267698A1-20101021-C00444
    Figure US20100267698A1-20101021-C00445
         		6.13 GC/FID
    239
    Figure US20100267698A1-20101021-C00446
    Figure US20100267698A1-20101021-C00447
    Figure US20100267698A1-20101021-C00448
         		5.97 GC/FID
    240
    Figure US20100267698A1-20101021-C00449
    Figure US20100267698A1-20101021-C00450
    Figure US20100267698A1-20101021-C00451
         		5.82 GC/FID
    241
    Figure US20100267698A1-20101021-C00452
    Figure US20100267698A1-20101021-C00453
    Figure US20100267698A1-20101021-C00454
         		6.51 GC/FID
    242
    Figure US20100267698A1-20101021-C00455
    Figure US20100267698A1-20101021-C00456
    Figure US20100267698A1-20101021-C00457
         		6.36 GC/FID
    243
    Figure US20100267698A1-20101021-C00458
    Figure US20100267698A1-20101021-C00459
    Figure US20100267698A1-20101021-C00460
         		6.42 GC/FID
    244
    Figure US20100267698A1-20101021-C00461
    Figure US20100267698A1-20101021-C00462
    Figure US20100267698A1-20101021-C00463
         		6.26 GC/FID
    245
    Figure US20100267698A1-20101021-C00464
    Figure US20100267698A1-20101021-C00465
    Figure US20100267698A1-20101021-C00466
         		6.52 GC/FID
    246
    Figure US20100267698A1-20101021-C00467
    Figure US20100267698A1-20101021-C00468
    Figure US20100267698A1-20101021-C00469
         		6.38 GC/FID
    247
    Figure US20100267698A1-20101021-C00470
    Figure US20100267698A1-20101021-C00471
    Figure US20100267698A1-20101021-C00472
         		6.34 GC/FID
    248
    Figure US20100267698A1-20101021-C00473
    Figure US20100267698A1-20101021-C00474
    Figure US20100267698A1-20101021-C00475
         		6.19 GC/FID
    249
    Figure US20100267698A1-20101021-C00476
    Figure US20100267698A1-20101021-C00477
    Figure US20100267698A1-20101021-C00478
         		6.45 GC/FID
    250
    Figure US20100267698A1-20101021-C00479
    Figure US20100267698A1-20101021-C00480
    Figure US20100267698A1-20101021-C00481
         		6.32 GC/FID
    251
    Figure US20100267698A1-20101021-C00482
    Figure US20100267698A1-20101021-C00483
    Figure US20100267698A1-20101021-C00484
         		6.71 GC/FID
    252
    Figure US20100267698A1-20101021-C00485
    Figure US20100267698A1-20101021-C00486
    Figure US20100267698A1-20101021-C00487
         		2.18 LC/MS
    253
    Figure US20100267698A1-20101021-C00488
    Figure US20100267698A1-20101021-C00489
    Figure US20100267698A1-20101021-C00490
         		6.28 GC/FID
    254
    Figure US20100267698A1-20101021-C00491
    Figure US20100267698A1-20101021-C00492
    Figure US20100267698A1-20101021-C00493
         		6.12 GC/FID
    255
    Figure US20100267698A1-20101021-C00494
    Figure US20100267698A1-20101021-C00495
    Figure US20100267698A1-20101021-C00496
         		7.38 GC/FID
    256
    Figure US20100267698A1-20101021-C00497
    Figure US20100267698A1-20101021-C00498
    Figure US20100267698A1-20101021-C00499
         		7.11 GC/FID
    257
    Figure US20100267698A1-20101021-C00500
    Figure US20100267698A1-20101021-C00501
    Figure US20100267698A1-20101021-C00502
         		1.48 LC/MS
    258
    Figure US20100267698A1-20101021-C00503
    Figure US20100267698A1-20101021-C00504
    Figure US20100267698A1-20101021-C00505
         		7.95 GC/FID
    259
    Figure US20100267698A1-20101021-C00506
    Figure US20100267698A1-20101021-C00507
    Figure US20100267698A1-20101021-C00508
         		6.34 GC/FID
    260
    Figure US20100267698A1-20101021-C00509
    Figure US20100267698A1-20101021-C00510
    Figure US20100267698A1-20101021-C00511
         		6.18 GC/FID
    261
    Figure US20100267698A1-20101021-C00512
    Figure US20100267698A1-20101021-C00513
    Figure US20100267698A1-20101021-C00514
         		6.47 GC/FID
    262
    Figure US20100267698A1-20101021-C00515
    Figure US20100267698A1-20101021-C00516
    Figure US20100267698A1-20101021-C00517
         		6.32 GC/FID
    263
    Figure US20100267698A1-20101021-C00518
    Figure US20100267698A1-20101021-C00519
    Figure US20100267698A1-20101021-C00520
         		6.10 GC/FID
    264
    Figure US20100267698A1-20101021-C00521
    Figure US20100267698A1-20101021-C00522
    Figure US20100267698A1-20101021-C00523
         		5.95 GC/FID
    265
    Figure US20100267698A1-20101021-C00524
    Figure US20100267698A1-20101021-C00525
    Figure US20100267698A1-20101021-C00526
         		6.15 GC/FID
    266
    Figure US20100267698A1-20101021-C00527
    Figure US20100267698A1-20101021-C00528
    Figure US20100267698A1-20101021-C00529
         		6.00 GC/FID
    267
    Figure US20100267698A1-20101021-C00530
    Figure US20100267698A1-20101021-C00531
    Figure US20100267698A1-20101021-C00532
         		7.43 GC/FID
    268
    Figure US20100267698A1-20101021-C00533
    Figure US20100267698A1-20101021-C00534
    Figure US20100267698A1-20101021-C00535
         		7.15 GC/FID
    269
    Figure US20100267698A1-20101021-C00536
    Figure US20100267698A1-20101021-C00537
    Figure US20100267698A1-20101021-C00538
         		7.74 GC/FID
    270
    Figure US20100267698A1-20101021-C00539
    Figure US20100267698A1-20101021-C00540
    Figure US20100267698A1-20101021-C00541
         		7.41 GC/FID
    271
    Figure US20100267698A1-20101021-C00542
    Figure US20100267698A1-20101021-C00543
    Figure US20100267698A1-20101021-C00544
         		6.65 GC/FID
    272
    Figure US20100267698A1-20101021-C00545
    Figure US20100267698A1-20101021-C00546
    Figure US20100267698A1-20101021-C00547
         		6.50 GC/FID
    273
    Figure US20100267698A1-20101021-C00548
    Figure US20100267698A1-20101021-C00549
    Figure US20100267698A1-20101021-C00550
         		6.23 GC/FID
    274
    Figure US20100267698A1-20101021-C00551
    Figure US20100267698A1-20101021-C00552
    Figure US20100267698A1-20101021-C00553
         		6.08 GC/FID
    275
    Figure US20100267698A1-20101021-C00554
    Figure US20100267698A1-20101021-C00555
    Figure US20100267698A1-20101021-C00556
         		6.30 GC/FID
    276
    Figure US20100267698A1-20101021-C00557
    Figure US20100267698A1-20101021-C00558
    Figure US20100267698A1-20101021-C00559
         		6.15 GC/FID
  • TABLE 4
    Figure US20100267698A1-20101021-C00560
    GC/FID
    Cmp. or
    # R2 R5 W1 RT Mass LC/MS
    277
    Figure US20100267698A1-20101021-C00561
    Figure US20100267698A1-20101021-C00562
    Figure US20100267698A1-20101021-C00563
         		7.12 GC/FID
    278
    Figure US20100267698A1-20101021-C00564
    Figure US20100267698A1-20101021-C00565
    Figure US20100267698A1-20101021-C00566
         		6.77 GC/FID
    279
    Figure US20100267698A1-20101021-C00567
    Figure US20100267698A1-20101021-C00568
    Figure US20100267698A1-20101021-C00569
         		7.17 GC/FID
    280
    Figure US20100267698A1-20101021-C00570
    Figure US20100267698A1-20101021-C00571
    Figure US20100267698A1-20101021-C00572
         		6.82 GC/FID
    281
    Figure US20100267698A1-20101021-C00573
    Figure US20100267698A1-20101021-C00574
    Figure US20100267698A1-20101021-C00575
         		7.10 GC/FID
    282
    Figure US20100267698A1-20101021-C00576
    Figure US20100267698A1-20101021-C00577
    Figure US20100267698A1-20101021-C00578
         		6.77 GC/FID
    283
    Figure US20100267698A1-20101021-C00579
    Figure US20100267698A1-20101021-C00580
    Figure US20100267698A1-20101021-C00581
         		7.30 GC/FID
    284
    Figure US20100267698A1-20101021-C00582
    Figure US20100267698A1-20101021-C00583
    Figure US20100267698A1-20101021-C00584
         		6.92 GC/FID
    285
    Figure US20100267698A1-20101021-C00585
    Figure US20100267698A1-20101021-C00586
    Figure US20100267698A1-20101021-C00587
         		7.53 GC/FID
    286
    Figure US20100267698A1-20101021-C00588
    Figure US20100267698A1-20101021-C00589
    Figure US20100267698A1-20101021-C00590
         		7.07 GC/FID
    287
    Figure US20100267698A1-20101021-C00591
    Figure US20100267698A1-20101021-C00592
    Figure US20100267698A1-20101021-C00593
         		7.79 GC/FID
    288
    Figure US20100267698A1-20101021-C00594
    Figure US20100267698A1-20101021-C00595
    Figure US20100267698A1-20101021-C00596
         		7.28 GC/FID
    289
    Figure US20100267698A1-20101021-C00597
    Figure US20100267698A1-20101021-C00598
    Figure US20100267698A1-20101021-C00599
         		7.29 GC/FID
    290
    Figure US20100267698A1-20101021-C00600
    Figure US20100267698A1-20101021-C00601
    Figure US20100267698A1-20101021-C00602
         		6.92 GC/FID
    291
    Figure US20100267698A1-20101021-C00603
    Figure US20100267698A1-20101021-C00604
    Figure US20100267698A1-20101021-C00605
         		7.86 GC/FID
    292
    Figure US20100267698A1-20101021-C00606
    Figure US20100267698A1-20101021-C00607
    Figure US20100267698A1-20101021-C00608
         		7.34 GC/FID
    293
    Figure US20100267698A1-20101021-C00609
    Figure US20100267698A1-20101021-C00610
    Figure US20100267698A1-20101021-C00611
         		7.77 GC/FID
    294
    Figure US20100267698A1-20101021-C00612
    Figure US20100267698A1-20101021-C00613
    Figure US20100267698A1-20101021-C00614
         		7.29 GC/FID
    295
    Figure US20100267698A1-20101021-C00615
    Figure US20100267698A1-20101021-C00616
    Figure US20100267698A1-20101021-C00617
         		1.44 472.4 LC/MS
    296
    Figure US20100267698A1-20101021-C00618
    Figure US20100267698A1-20101021-C00619
    Figure US20100267698A1-20101021-C00620
         		1.56 508.4 LC/MS
    297
    Figure US20100267698A1-20101021-C00621
    Figure US20100267698A1-20101021-C00622
    Figure US20100267698A1-20101021-C00623
         		2.02 468.5 LC/MS
    298
    Figure US20100267698A1-20101021-C00624
    Figure US20100267698A1-20101021-C00625
    Figure US20100267698A1-20101021-C00626
         		7.84 GC/FID
    299
    Figure US20100267698A1-20101021-C00627
    Figure US20100267698A1-20101021-C00628
    Figure US20100267698A1-20101021-C00629
         		6.41 GC/FID
    300
    Figure US20100267698A1-20101021-C00630
    Figure US20100267698A1-20101021-C00631
    Figure US20100267698A1-20101021-C00632
         		1.32 410.3 LC/MS
    301
    Figure US20100267698A1-20101021-C00633
    Figure US20100267698A1-20101021-C00634
    Figure US20100267698A1-20101021-C00635
         		6.59 GC/FID
    302
    Figure US20100267698A1-20101021-C00636
    Figure US20100267698A1-20101021-C00637
    Figure US20100267698A1-20101021-C00638
         		6.35 GC/FID
    303
    Figure US20100267698A1-20101021-C00639
    Figure US20100267698A1-20101021-C00640
    Figure US20100267698A1-20101021-C00641
         		6.54 GC/FID
    304
    Figure US20100267698A1-20101021-C00642
    Figure US20100267698A1-20101021-C00643
    Figure US20100267698A1-20101021-C00644
         		6.29 GC/FID
    305
    Figure US20100267698A1-20101021-C00645
    Figure US20100267698A1-20101021-C00646
    Figure US20100267698A1-20101021-C00647
         		6.61 GC/FID
    306
    Figure US20100267698A1-20101021-C00648
    Figure US20100267698A1-20101021-C00649
    Figure US20100267698A1-20101021-C00650
         		6.37 GC/FID
    307
    Figure US20100267698A1-20101021-C00651
    Figure US20100267698A1-20101021-C00652
    Figure US20100267698A1-20101021-C00653
         		6.76 GC/FID
    308
    Figure US20100267698A1-20101021-C00654
    Figure US20100267698A1-20101021-C00655
    Figure US20100267698A1-20101021-C00656
         		6.50 GC/FID
    309
    Figure US20100267698A1-20101021-C00657
    Figure US20100267698A1-20101021-C00658
    Figure US20100267698A1-20101021-C00659
         		6.58 GC/FID
    310
    Figure US20100267698A1-20101021-C00660
    Figure US20100267698A1-20101021-C00661
    Figure US20100267698A1-20101021-C00662
         		6.34 GC/FID
    311
    Figure US20100267698A1-20101021-C00663
    Figure US20100267698A1-20101021-C00664
    Figure US20100267698A1-20101021-C00665
         		6.72 GC/FID
    312
    Figure US20100267698A1-20101021-C00666
    Figure US20100267698A1-20101021-C00667
    Figure US20100267698A1-20101021-C00668
         		6.47 GC/FID
    313
    Figure US20100267698A1-20101021-C00669
    Figure US20100267698A1-20101021-C00670
    Figure US20100267698A1-20101021-C00671
         		6.74 GC/FID
    314
    Figure US20100267698A1-20101021-C00672
    Figure US20100267698A1-20101021-C00673
    Figure US20100267698A1-20101021-C00674
         		6.50 GC/FID
    315
    Figure US20100267698A1-20101021-C00675
    Figure US20100267698A1-20101021-C00676
    Figure US20100267698A1-20101021-C00677
         		6.78 GC/FID
    316
    Figure US20100267698A1-20101021-C00678
    Figure US20100267698A1-20101021-C00679
    Figure US20100267698A1-20101021-C00680
         		6.52 GC/FID
    317
    Figure US20100267698A1-20101021-C00681
    Figure US20100267698A1-20101021-C00682
    Figure US20100267698A1-20101021-C00683
         		7.05 GC/FID
    318
    Figure US20100267698A1-20101021-C00684
    Figure US20100267698A1-20101021-C00685
    Figure US20100267698A1-20101021-C00686
         		6.74 GC/FID
    319
    Figure US20100267698A1-20101021-C00687
    Figure US20100267698A1-20101021-C00688
    Figure US20100267698A1-20101021-C00689
         		7.13 GC/FID
    320
    Figure US20100267698A1-20101021-C00690
    Figure US20100267698A1-20101021-C00691
    Figure US20100267698A1-20101021-C00692
         		2.14 480.5 LC/MS
    321
    Figure US20100267698A1-20101021-C00693
    Figure US20100267698A1-20101021-C00694
    Figure US20100267698A1-20101021-C00695
         		6.96 GC/FID
    322
    Figure US20100267698A1-20101021-C00696
    Figure US20100267698A1-20101021-C00697
    Figure US20100267698A1-20101021-C00698
         		6.67 GC/FID
    323
    Figure US20100267698A1-20101021-C00699
    Figure US20100267698A1-20101021-C00700
    Figure US20100267698A1-20101021-C00701
         		6.49 GC/FID
    324
    Figure US20100267698A1-20101021-C00702
    Figure US20100267698A1-20101021-C00703
    Figure US20100267698A1-20101021-C00704
         		6.26 GC/FID
    325
    Figure US20100267698A1-20101021-C00705
    Figure US20100267698A1-20101021-C00706
    Figure US20100267698A1-20101021-C00707
         		7.13 GC/FID
    326
    Figure US20100267698A1-20101021-C00708
    Figure US20100267698A1-20101021-C00709
    Figure US20100267698A1-20101021-C00710
         		6.77 GC/FID
    327
    Figure US20100267698A1-20101021-C00711
    Figure US20100267698A1-20101021-C00712
    Figure US20100267698A1-20101021-C00713
         		6.65 GC/FID
    328
    Figure US20100267698A1-20101021-C00714
    Figure US20100267698A1-20101021-C00715
    Figure US20100267698A1-20101021-C00716
         		6.42 GC/FID
    329
    Figure US20100267698A1-20101021-C00717
    Figure US20100267698A1-20101021-C00718
    Figure US20100267698A1-20101021-C00719
         		7.39 GC/FID
    330
    Figure US20100267698A1-20101021-C00720
    Figure US20100267698A1-20101021-C00721
    Figure US20100267698A1-20101021-C00722
         		7.00 GC/FID
    331
    Figure US20100267698A1-20101021-C00723
    Figure US20100267698A1-20101021-C00724
    Figure US20100267698A1-20101021-C00725
         		7.26 GC/FID
    332
    Figure US20100267698A1-20101021-C00726
    Figure US20100267698A1-20101021-C00727
    Figure US20100267698A1-20101021-C00728
         		6.90 GC/FID
    333
    Figure US20100267698A1-20101021-C00729
    Figure US20100267698A1-20101021-C00730
    Figure US20100267698A1-20101021-C00731
         		1.99 456.5 LC/MS
    334
    Figure US20100267698A1-20101021-C00732
    Figure US20100267698A1-20101021-C00733
    Figure US20100267698A1-20101021-C00734
         		2.06 492.5 LC/MS
    335
    Figure US20100267698A1-20101021-C00735
    Figure US20100267698A1-20101021-C00736
    Figure US20100267698A1-20101021-C00737
         		7.00 GC/FID
    336
    Figure US20100267698A1-20101021-C00738
    Figure US20100267698A1-20101021-C00739
    Figure US20100267698A1-20101021-C00740
         		6.71 GC/FID
    337
    Figure US20100267698A1-20101021-C00741
    Figure US20100267698A1-20101021-C00742
    Figure US20100267698A1-20101021-C00743
         		7.31 GC/FID
    338
    Figure US20100267698A1-20101021-C00744
    Figure US20100267698A1-20101021-C00745
    Figure US20100267698A1-20101021-C00746
         		2.45 545.6 LC/MS
    339
    Figure US20100267698A1-20101021-C00747
    Figure US20100267698A1-20101021-C00748
    Figure US20100267698A1-20101021-C00749
         		7.86 GC/FID
    340
    Figure US20100267698A1-20101021-C00750
    Figure US20100267698A1-20101021-C00751
    Figure US20100267698A1-20101021-C00752
         		2.65 550.7 LC/MS
    341
    Figure US20100267698A1-20101021-C00753
    Figure US20100267698A1-20101021-C00754
    Figure US20100267698A1-20101021-C00755
         		7.14 GC/FID
    342
    Figure US20100267698A1-20101021-C00756
    Figure US20100267698A1-20101021-C00757
    Figure US20100267698A1-20101021-C00758
         		6.81 GC/FID
    343
    Figure US20100267698A1-20101021-C00759
    Figure US20100267698A1-20101021-C00760
    Figure US20100267698A1-20101021-C00761
         		9.51 GC/FID
    344
    Figure US20100267698A1-20101021-C00762
    Figure US20100267698A1-20101021-C00763
    Figure US20100267698A1-20101021-C00764
         		8.54 GC/FID
    345
    Figure US20100267698A1-20101021-C00765
    Figure US20100267698A1-20101021-C00766
    Figure US20100267698A1-20101021-C00767
         		11.94 GC/FID
    346
    Figure US20100267698A1-20101021-C00768
    Figure US20100267698A1-20101021-C00769
    Figure US20100267698A1-20101021-C00770
         		1.48 547.7 LC/MS
    347
    Figure US20100267698A1-20101021-C00771
    Figure US20100267698A1-20101021-C00772
    Figure US20100267698A1-20101021-C00773
         		7.22 GC/FID
    348
    Figure US20100267698A1-20101021-C00774
    Figure US20100267698A1-20101021-C00775
    Figure US20100267698A1-20101021-C00776
         		6.83 GC/FID
    349
    Figure US20100267698A1-20101021-C00777
    Figure US20100267698A1-20101021-C00778
    Figure US20100267698A1-20101021-C00779
         		7.44 GC/FID
    350
    Figure US20100267698A1-20101021-C00780
    Figure US20100267698A1-20101021-C00781
    Figure US20100267698A1-20101021-C00782
         		7.04 GC/FID
    351
    Figure US20100267698A1-20101021-C00783
    Figure US20100267698A1-20101021-C00784
    Figure US20100267698A1-20101021-C00785
         		6.83 GC/FID
    352
    Figure US20100267698A1-20101021-C00786
    Figure US20100267698A1-20101021-C00787
    Figure US20100267698A1-20101021-C00788
         		6.56 GC/FID
    353
    Figure US20100267698A1-20101021-C00789
    Figure US20100267698A1-20101021-C00790
    Figure US20100267698A1-20101021-C00791
         		6.84 GC/FID
    354
    Figure US20100267698A1-20101021-C00792
    Figure US20100267698A1-20101021-C00793
    Figure US20100267698A1-20101021-C00794
         		6.58 GC/FID
    355
    Figure US20100267698A1-20101021-C00795
    Figure US20100267698A1-20101021-C00796
    Figure US20100267698A1-20101021-C00797
         		9.63 GC/FID
    356
    Figure US20100267698A1-20101021-C00798
    Figure US20100267698A1-20101021-C00799
    Figure US20100267698A1-20101021-C00800
         		8.64 GC/FID
    357
    Figure US20100267698A1-20101021-C00801
    Figure US20100267698A1-20101021-C00802
    Figure US20100267698A1-20101021-C00803
         		1.05 497.6 LC/MS
    358
    Figure US20100267698A1-20101021-C00804
    Figure US20100267698A1-20101021-C00805
    Figure US20100267698A1-20101021-C00806
         		9.16 GC/FID
    359
    Figure US20100267698A1-20101021-C00807
    Figure US20100267698A1-20101021-C00808
    Figure US20100267698A1-20101021-C00809
         		7.61 GC/FID
    360
    Figure US20100267698A1-20101021-C00810
    Figure US20100267698A1-20101021-C00811
    Figure US20100267698A1-20101021-C00812
         		7.17 GC/FID
    361
    Figure US20100267698A1-20101021-C00813
    Figure US20100267698A1-20101021-C00814
    Figure US20100267698A1-20101021-C00815
         		7.02 GC/FID
    362
    Figure US20100267698A1-20101021-C00816
    Figure US20100267698A1-20101021-C00817
    Figure US20100267698A1-20101021-C00818
         		6.72 GC/FID
    363
    Figure US20100267698A1-20101021-C00819
    Figure US20100267698A1-20101021-C00820
    Figure US20100267698A1-20101021-C00821
         		1.05 459.5 LC/MS
    364
    Figure US20100267698A1-20101021-C00822
    Figure US20100267698A1-20101021-C00823
    Figure US20100267698A1-20101021-C00824
         		6.79 GC/FID
  • TABLE 5
    Figure US20100267698A1-20101021-C00825
    GC/FID
    Cpd. or
    # R2 R5 W1 MW RT Mass LC/MS
    365
    Figure US20100267698A1-20101021-C00826
    Figure US20100267698A1-20101021-C00827
    Figure US20100267698A1-20101021-C00828
         		413.5223 NA
    366
    Figure US20100267698A1-20101021-C00829
    Figure US20100267698A1-20101021-C00830
    Figure US20100267698A1-20101021-C00831
         		431.5124 1.73 432.1 LC/MS
    367
    Figure US20100267698A1-20101021-C00832
    Figure US20100267698A1-20101021-C00833
    Figure US20100267698A1-20101021-C00834
         		449.5025 1.67 450.4 LC/MS
    368
    Figure US20100267698A1-20101021-C00835
    Figure US20100267698A1-20101021-C00836
    Figure US20100267698A1-20101021-C00837
         		447.9674 1.87 448.3 LC/MS
    369
    Figure US20100267698A1-20101021-C00838
    Figure US20100267698A1-20101021-C00839
    Figure US20100267698A1-20101021-C00840
         		427.5491 1.87 428.4 LC/MS
    370
    Figure US20100267698A1-20101021-C00841
    Figure US20100267698A1-20101021-C00842
    Figure US20100267698A1-20101021-C00843
         		445.5392 1.94 446.4 LC/MS
    371
    Figure US20100267698A1-20101021-C00844
    Figure US20100267698A1-20101021-C00845
    Figure US20100267698A1-20101021-C00846
         		463.5293 1.87 464.4 LC/MS
    365
    Figure US20100267698A1-20101021-C00847
    Figure US20100267698A1-20101021-C00848
    Figure US20100267698A1-20101021-C00849
         		413.5223 NA
    366
    Figure US20100267698A1-20101021-C00850
    Figure US20100267698A1-20101021-C00851
    Figure US20100267698A1-20101021-C00852
         		431.5124 1.73 432.1 LC/MS
    367
    Figure US20100267698A1-20101021-C00853
    Figure US20100267698A1-20101021-C00854
    Figure US20100267698A1-20101021-C00855
         		449.5025 1.67 450.4 LC/MS
    368
    Figure US20100267698A1-20101021-C00856
    Figure US20100267698A1-20101021-C00857
    Figure US20100267698A1-20101021-C00858
         		447.9674 1.87 448.3 LC/MS
    369
    Figure US20100267698A1-20101021-C00859
    Figure US20100267698A1-20101021-C00860
    Figure US20100267698A1-20101021-C00861
         		427.5491 1.87 428.4 LC/MS
    370
    Figure US20100267698A1-20101021-C00862
    Figure US20100267698A1-20101021-C00863
    Figure US20100267698A1-20101021-C00864
         		445.5392 1.94 446.4 LC/MS
    371
    Figure US20100267698A1-20101021-C00865
    Figure US20100267698A1-20101021-C00866
    Figure US20100267698A1-20101021-C00867
         		463.5293 1.87 464.4 LC/MS
    372
    Figure US20100267698A1-20101021-C00868
    Figure US20100267698A1-20101021-C00869
    Figure US20100267698A1-20101021-C00870
         		461.9942 2.07 462.4 LC/MS
    373
    Figure US20100267698A1-20101021-C00871
    Figure US20100267698A1-20101021-C00872
    Figure US20100267698A1-20101021-C00873
         		429.5213 2.87 430 LC/MS
    374
    Figure US20100267698A1-20101021-C00874
    Figure US20100267698A1-20101021-C00875
    Figure US20100267698A1-20101021-C00876
         		447.5114 1.53 448.3 LC/MS
    375
    Figure US20100267698A1-20101021-C00877
    Figure US20100267698A1-20101021-C00878
    Figure US20100267698A1-20101021-C00879
         		465.5015 1.47 466.5 LC/MS
    376
    Figure US20100267698A1-20101021-C00880
    Figure US20100267698A1-20101021-C00881
    Figure US20100267698A1-20101021-C00882
         		463.9664 1.67 464.4 LC/MS
    377
    Figure US20100267698A1-20101021-C00883
    Figure US20100267698A1-20101021-C00884
    Figure US20100267698A1-20101021-C00885
         		441.5759 2.07 442.6 LC/MS
    378
    Figure US20100267698A1-20101021-C00886
    Figure US20100267698A1-20101021-C00887
    Figure US20100267698A1-20101021-C00888
         		459.566 2.13 460.3 LC/MS
    379
    Figure US20100267698A1-20101021-C00889
    Figure US20100267698A1-20101021-C00890
    Figure US20100267698A1-20101021-C00891
         		477.5561 NA
    380
    Figure US20100267698A1-20101021-C00892
    Figure US20100267698A1-20101021-C00893
    Figure US20100267698A1-20101021-C00894
         		441.5759 2.00 442.6 LC/MS
    381
    Figure US20100267698A1-20101021-C00895
    Figure US20100267698A1-20101021-C00896
    Figure US20100267698A1-20101021-C00897
         		459.566 2.07 460.3 LC/MS
    382
    Figure US20100267698A1-20101021-C00898
    Figure US20100267698A1-20101021-C00899
    Figure US20100267698A1-20101021-C00900
         		445.5883 1.73 446.4 LC/MS
    383
    Figure US20100267698A1-20101021-C00901
    Figure US20100267698A1-20101021-C00902
    Figure US20100267698A1-20101021-C00903
         		463.5784 1.80 464.4 LC/MS
    384
    Figure US20100267698A1-20101021-C00904
    Figure US20100267698A1-20101021-C00905
    Figure US20100267698A1-20101021-C00906
         		481.5685 1.73 482.2 LC/MS
    385
    Figure US20100267698A1-20101021-C00907
    Figure US20100267698A1-20101021-C00908
    Figure US20100267698A1-20101021-C00909
         		480.0334 NA
    386
    Figure US20100267698A1-20101021-C00910
    Figure US20100267698A1-20101021-C00911
    Figure US20100267698A1-20101021-C00912
         		455.6027 2.13 456.4 LC/MS
    387
    Figure US20100267698A1-20101021-C00913
    Figure US20100267698A1-20101021-C00914
    Figure US20100267698A1-20101021-C00915
         		473.5928 NA
    388
    Figure US20100267698A1-20101021-C00916
    Figure US20100267698A1-20101021-C00917
    Figure US20100267698A1-20101021-C00918
         		491.5829 2.13 492.4 LC/MS
    389
    Figure US20100267698A1-20101021-C00919
    Figure US20100267698A1-20101021-C00920
    Figure US20100267698A1-20101021-C00921
         		490.0478 2.27 490.3 LC/MS
    390
    Figure US20100267698A1-20101021-C00922
    Figure US20100267698A1-20101021-C00923
    Figure US20100267698A1-20101021-C00924
         		455.6027 2.13 456.4 LC/MS
    391
    Figure US20100267698A1-20101021-C00925
    Figure US20100267698A1-20101021-C00926
    Figure US20100267698A1-20101021-C00927
         		473.5928 NA
    392
    Figure US20100267698A1-20101021-C00928
    Figure US20100267698A1-20101021-C00929
    Figure US20100267698A1-20101021-C00930
         		491.5829 2.13 492.4 LC/MS
    393
    Figure US20100267698A1-20101021-C00931
    Figure US20100267698A1-20101021-C00932
    Figure US20100267698A1-20101021-C00933
         		490.0478 2.27 490.5 LC/MS
    394
    Figure US20100267698A1-20101021-C00934
    Figure US20100267698A1-20101021-C00935
    Figure US20100267698A1-20101021-C00936
         		483.6563 2.40 484.5 LC/MS
    395
    Figure US20100267698A1-20101021-C00937
    Figure US20100267698A1-20101021-C00938
    Figure US20100267698A1-20101021-C00939
         		501.6464 2.47 502.3 LC/MS
    396
    Figure US20100267698A1-20101021-C00940
    Figure US20100267698A1-20101021-C00941
    Figure US20100267698A1-20101021-C00942
         		519.6365 NA
    397
    Figure US20100267698A1-20101021-C00943
    Figure US20100267698A1-20101021-C00944
    Figure US20100267698A1-20101021-C00945
         		518.1014 NA
    398
    Figure US20100267698A1-20101021-C00946
    Figure US20100267698A1-20101021-C00947
    Figure US20100267698A1-20101021-C00948
         		485.5849 1.73 486.3 LC/MS
    399
    Figure US20100267698A1-20101021-C00949
    Figure US20100267698A1-20101021-C00950
    Figure US20100267698A1-20101021-C00951
         		503.575 1.73 504.3 LC/MS
    400
    Figure US20100267698A1-20101021-C00952
    Figure US20100267698A1-20101021-C00953
    Figure US20100267698A1-20101021-C00954
         		521.5651 NA
    401
    Figure US20100267698A1-20101021-C00955
    Figure US20100267698A1-20101021-C00956
    Figure US20100267698A1-20101021-C00957
         		520.03 1.87 520.2 LC/MS
    402
    Figure US20100267698A1-20101021-C00958
    Figure US20100267698A1-20101021-C00959
    Figure US20100267698A1-20101021-C00960
         		481.6405 2.34 482.5 LC/MS
    403
    Figure US20100267698A1-20101021-C00961
    Figure US20100267698A1-20101021-C00962
    Figure US20100267698A1-20101021-C00963
         		499.6306 2.40 500.4 LC/MS
    404
    Figure US20100267698A1-20101021-C00964
    Figure US20100267698A1-20101021-C00965
    Figure US20100267698A1-20101021-C00966
         		517.6207 2.34 518 LC/MS
    405
    Figure US20100267698A1-20101021-C00967
    Figure US20100267698A1-20101021-C00968
    Figure US20100267698A1-20101021-C00969
         		516.0856 2.47 516.4 LC/MS
    406
    Figure US20100267698A1-20101021-C00970
    Figure US20100267698A1-20101021-C00971
    Figure US20100267698A1-20101021-C00972
         		387.4845 1.47 388.5 LC/MS
    407
    Figure US20100267698A1-20101021-C00973
    Figure US20100267698A1-20101021-C00974
    Figure US20100267698A1-20101021-C00975
         		405.4746 1.53 406.3 LC/MS
    408
    Figure US20100267698A1-20101021-C00976
    Figure US20100267698A1-20101021-C00977
    Figure US20100267698A1-20101021-C00978
         		423.4647 1.47 424.2 LC/MS
    409
    Figure US20100267698A1-20101021-C00979
    Figure US20100267698A1-20101021-C00980
    Figure US20100267698A1-20101021-C00981
         		413.5223 1.80 414.4 LC/MS
    410
    Figure US20100267698A1-20101021-C00982
    Figure US20100267698A1-20101021-C00983
    Figure US20100267698A1-20101021-C00984
         		431.5124 1.87 432.1 LC/MS
    411
    Figure US20100267698A1-20101021-C00985
    Figure US20100267698A1-20101021-C00986
    Figure US20100267698A1-20101021-C00987
         		449.5025 1.73 450.3 LC/MS
    412
    Figure US20100267698A1-20101021-C00988
    Figure US20100267698A1-20101021-C00989
    Figure US20100267698A1-20101021-C00990
         		447.9674 1.93 448.5 LC/MS
    413
    Figure US20100267698A1-20101021-C00991
    Figure US20100267698A1-20101021-C00992
    Figure US20100267698A1-20101021-C00993
         		415.5381 1.80 416.4 LC/MS
    414
    Figure US20100267698A1-20101021-C00994
    Figure US20100267698A1-20101021-C00995
    Figure US20100267698A1-20101021-C00996
         		433.5282 1.87 434.5 LC/MS
    415
    Figure US20100267698A1-20101021-C00997
    Figure US20100267698A1-20101021-C00998
    Figure US20100267698A1-20101021-C00999
         		451.5183 1.80 452.4 LC/MS
    416
    Figure US20100267698A1-20101021-C01000
    Figure US20100267698A1-20101021-C01001
    Figure US20100267698A1-20101021-C01002
         		449.9832 2.00 450.3 LC/MS
    417
    Figure US20100267698A1-20101021-C01003
    Figure US20100267698A1-20101021-C01004
    Figure US20100267698A1-20101021-C01005
         		415.5381 1.80 416.4 LC/MS
    418
    Figure US20100267698A1-20101021-C01006
    Figure US20100267698A1-20101021-C01007
    Figure US20100267698A1-20101021-C01008
         		433.5282 1.87 434.5 LC/MS
    419
    Figure US20100267698A1-20101021-C01009
    Figure US20100267698A1-20101021-C01010
    Figure US20100267698A1-20101021-C01011
         		451.5183 1.80 452.4 LC/MS
    420
    Figure US20100267698A1-20101021-C01012
    Figure US20100267698A1-20101021-C01013
    Figure US20100267698A1-20101021-C01014
         		449.9832 2.00 450.3 LC/MS
    421
    Figure US20100267698A1-20101021-C01015
    Figure US20100267698A1-20101021-C01016
    Figure US20100267698A1-20101021-C01017
         		429.5649 2.00 430.2 LC/MS
    422
    Figure US20100267698A1-20101021-C01018
    Figure US20100267698A1-20101021-C01019
    Figure US20100267698A1-20101021-C01020
         		447.555 2.07 448.6 LC/MS
    423
    Figure US20100267698A1-20101021-C01021
    Figure US20100267698A1-20101021-C01022
    Figure US20100267698A1-20101021-C01023
         		465.5451 2.00 466.3 LC/MS
    424
    Figure US20100267698A1-20101021-C01024
    Figure US20100267698A1-20101021-C01025
    Figure US20100267698A1-20101021-C01026
         		464.01 2.20 464.4 LC/MS
    425
    Figure US20100267698A1-20101021-C01027
    Figure US20100267698A1-20101021-C01028
    Figure US20100267698A1-20101021-C01029
         		429.5649 1.93 430.2 LC/MS
    426
    Figure US20100267698A1-20101021-C01030
    Figure US20100267698A1-20101021-C01031
    Figure US20100267698A1-20101021-C01032
         		447.555 2.00 448.6 LC/MS
    427
    Figure US20100267698A1-20101021-C01033
    Figure US20100267698A1-20101021-C01034
    Figure US20100267698A1-20101021-C01035
         		465.5451 1.93 466.3 LC/MS
    428
    Figure US20100267698A1-20101021-C01036
    Figure US20100267698A1-20101021-C01037
    Figure US20100267698A1-20101021-C01038
         		464.01 2.13 464.4 LC/MS
    429
    Figure US20100267698A1-20101021-C01039
    Figure US20100267698A1-20101021-C01040
    Figure US20100267698A1-20101021-C01041
         		439.5601 2.00 440.7 LC/MS
    430
    Figure US20100267698A1-20101021-C01042
    Figure US20100267698A1-20101021-C01043
    Figure US20100267698A1-20101021-C01044
         		457.5502 2.07 458.4 LC/MS
    431
    Figure US20100267698A1-20101021-C01045
    Figure US20100267698A1-20101021-C01046
    Figure US20100267698A1-20101021-C01047
         		475.5403 NA
    432
    Figure US20100267698A1-20101021-C01048
    Figure US20100267698A1-20101021-C01049
    Figure US20100267698A1-20101021-C01050
         		474.0052 NA
    433
    Figure US20100267698A1-20101021-C01051
    Figure US20100267698A1-20101021-C01052
    Figure US20100267698A1-20101021-C01053
         		443.5917 2.13 444.6 LC/MS
    434
    Figure US20100267698A1-20101021-C01054
    Figure US20100267698A1-20101021-C01055
    Figure US20100267698A1-20101021-C01056
         		461.5818 2.20 462.4 LC/MS
    435
    Figure US20100267698A1-20101021-C01057
    Figure US20100267698A1-20101021-C01058
    Figure US20100267698A1-20101021-C01059
         		479.5719 NA
    436
    Figure US20100267698A1-20101021-C01060
    Figure US20100267698A1-20101021-C01061
    Figure US20100267698A1-20101021-C01062
         		478.0368 NA
    437
    Figure US20100267698A1-20101021-C01063
    Figure US20100267698A1-20101021-C01064
    Figure US20100267698A1-20101021-C01065
         		443.5917 2.13 444.6 LC/MS
    438
    Figure US20100267698A1-20101021-C01066
    Figure US20100267698A1-20101021-C01067
    Figure US20100267698A1-20101021-C01068
         		461.5818 2.20 462.4 LC/MS
    439
    Figure US20100267698A1-20101021-C01069
    Figure US20100267698A1-20101021-C01070
    Figure US20100267698A1-20101021-C01071
         		479.5719 NA
    440
    Figure US20100267698A1-20101021-C01072
    Figure US20100267698A1-20101021-C01073
    Figure US20100267698A1-20101021-C01074
         		478.0368 NA
    441
    Figure US20100267698A1-20101021-C01075
    Figure US20100267698A1-20101021-C01076
    Figure US20100267698A1-20101021-C01077
         		455.6027 2.20 456.4 LC/MS
    442
    Figure US20100267698A1-20101021-C01078
    Figure US20100267698A1-20101021-C01079
    Figure US20100267698A1-20101021-C01080
         		473.5928 NA
    443
    Figure US20100267698A1-20101021-C01081
    Figure US20100267698A1-20101021-C01082
    Figure US20100267698A1-20101021-C01083
         		491.5829 NA
    444
    Figure US20100267698A1-20101021-C01084
    Figure US20100267698A1-20101021-C01085
    Figure US20100267698A1-20101021-C01086
         		490.0478 2.34 490.3 LC/MS
    445
    Figure US20100267698A1-20101021-C01087
    Figure US20100267698A1-20101021-C01088
    Figure US20100267698A1-20101021-C01089
         		457.6185 2.34 458.4 LC/MS
    446
    Figure US20100267698A1-20101021-C01090
    Figure US20100267698A1-20101021-C01091
    Figure US20100267698A1-20101021-C01092
         		475.6086 NA
    447
    Figure US20100267698A1-20101021-C01093
    Figure US20100267698A1-20101021-C01094
    Figure US20100267698A1-20101021-C01095
         		593.5987 2.34 494.5 LC/MS
    448
    Figure US20100267698A1-20101021-C01096
    Figure US20100267698A1-20101021-C01097
    Figure US20100267698A1-20101021-C01098
         		492.0636 2.47 492.4 LC/MS
  • TABLE 6
    Cpd# STRUCTURE IUPAC NAME NMR MS (m/z)
    449
    Figure US20100267698A1-20101021-C01099
         		2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-1- propyl-1H-benzimidazole 1H NMR (CDCl3): 7.80 (m, 1H), 7.41-7.53 (m, 3H), 7.29-7.31 (m, 3H), 7.14-7.22 (m, 2H), 7.06 (d, 1H), 5.51 (s, 2H), 3.69 (t, 2H,), 1.43 (m, 2H), 0.74 (t, 3H)
    450
    Figure US20100267698A1-20101021-C01100
         		2-{[2-(2-fluorophenyl)-1H- imidazol-1-yl]methyl}-1- propyl-1H-benzimidazole 7.78 (m, 1H), 7.66 (m, 1H), 7.51 (m, 1H), 7.23-7.37 (m, 5H), 7.17 (d, 1H), 7.03 (d, 1H), 5.35 (s, 2H), 3.68 (t, 2H), 1.40 (m, 2H), 0.72 (t, 3H)
    451
    Figure US20100267698A1-20101021-C01101
         		2-{[2-(4-fluorophenyl)-1H- imidazol-1-yl]methyl}-1- propyl-1H-benzimidazole 1H NMR (CDCl3): 7.80 (m, 1H), 7.65-7.69 (m, 2H), 7.18-7.32 (m, 5H), 7.13 (d, 1H), 7.04 (d, 1H), 5.47 (s, 2H), 3.68 (t, 2H,), 1.42 (m, 2H), 0.74 (t, 3H)
    452
    Figure US20100267698A1-20101021-C01102
         		2-{[2-(3-chloro-4-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1- propyl-1H-benzimidazole 1H NMR (CDCl3): 7.76-7.81 (m, 2H), 7.56 (m, 1H), 7.25-7.32 (m, 4H), 7.14 (d, 1H), 7.05 (d, 1H), 5.46 (s, 2H), 3.74 (t, 2H,), 1.47 (m, 2H), 0.78 (t, 3H)
    453
    Figure US20100267698A1-20101021-C01103
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-1- propyl-1H-benzimidazole 7.78 (m, 1H), 7.38 (m, 1H), 7.17- 7.31 (m, 6H), 7.03 (d, 1H), 5.36 (s, 2H), 3.73 (t, 2H), 1.44 (m, 2H), 0.76 (t, 3H)
    454
    Figure US20100267698A1-20101021-C01104
         		6-chloro-2-{[2-(2-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1- propyl-1H-benzimidazole 1H NMR (CDCl3): 7.62-7.69 (m, 2H), 7.51 (m, 1H), 7.22-7.34 (m, 4H), 7.18 (d, 1H), 7.02 (d, 1H), 5.34 (s, 2H), 3.64 (t, 2H,), 1.40 (m, 2H), 0.73 (t, 3H)
    455
    Figure US20100267698A1-20101021-C01105
         		6-chloro-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1- propyl-1H-benzimidazole 1H NMR (CDCl3): 7.69 (d, 1H), 7.38-7.52 (m, 3H), 7.15-7.29 (m, 4H), 7.04 (d, 1H), 5.48 (s, 2H), 3.65 (t, 2H), 1.42 (m, 2H), 0.75 (t, 3H)
    456
    Figure US20100267698A1-20101021-C01106
         		6-chloro-2-{[2-(4-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1- propyl-1H-benzimidazole 1H NMR (CDCl3): 7.62-7.70 (m, 3H), 7.13-7.28 (m, 5H), 7.02 (d, 1H), 3.64 (t, 2H), 1.41 (m, 2H), 0.75 (t, 3H)
    457
    Figure US20100267698A1-20101021-C01107
         		6-chloro-2-{[2-(3-chloro-4- fluorophenyl)-1H-imidazol-1- yl]methyl}-1-propyl-1H- benzimidazole 1H NMR (CDCl3): 7.75 (dd, 1H), 7.69 (dd, 1H), 7.55 (m, 1H), 7.24- 7.30 (m, 3H), 7.15 (d, 1H), 7.03 (d, 1H), 5.43 (s, 2H,), 3.71 (t, 2H), 1.47 (m, 2H), 0.79 (t, 3H)
    458
    Figure US20100267698A1-20101021-C01108
         		6-chloro-2-{[2-(2,5- difluorophenyl)-1H-imidazol-1- yl]methyl}-1-propyl-1H- benzimidazole 1H NMR (CDCl3): 7.67 (d, 1H), 7.37 (m, 1H), 7.17-7.29 (m, 5H), 7.02 (d, 1H), 5.34 (s, 2H), 3.69 (t, 2H), 1.43 (m, 2H), 0.77 (t, 3H)
    459
    Figure US20100267698A1-20101021-C01109
         		2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-3-propyl- 3H-imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.40 (dd, 1H), 8.06 (dd, 1H), 7.39-7.50 (m, 3H), 7.15-7.29 (m, 3H), 7.08 (d, 1H), 5.52 (s, 2H), 3.88 (t, 2H,), 1.51 (m, 2H), 0.77 (t, 3H)
    460
    Figure US20100267698A1-20101021-C01110
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-3-propyl- 3H-imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.40 (dd, 1H), 8.05 (dd, 1H), 7.38 (m, 1H), 7.17- 7.28 (m, 4H), 7.08 (d, 1H), 5.39 (s, 2H), 3.89 (t, 2H), 1.50 (m, 2H), 0.79 (t, 3H)
    461
    Figure US20100267698A1-20101021-C01111
         		6-chloro-1-(cyclopropylmethyl)- 2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-1H- benzimidazole 1H NMR (CDCl3): 7.68 (d, 1H), 7.38 (m, 1H), 7.32 (d, 1H), 7.19- 7.28 (m, 4H), 7.04 (d, 1H), 5.36 (d, 2H), 3.62 (d, 2H), 0.65 (m, 1H), 0.46-0.51 (m, 2H), 0.07-0.11 (m, 2H)
    462
    Figure US20100267698A1-20101021-C01112
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-3- isobutyl-3H-imidazo[4,5- b]pyridine 1H NMR (CDCl3): 8.38 (dd, 1H), 8.04 (dd, 1H), 7.38 (m, 1H), 7.16- 7.27 (m, 4H), 7.09 (d, 1H), 5.38 (s, 2H), 3.73 (d, 2H), 1.98 (m, 1H), 0.72 (d, 6H)
    463
    Figure US20100267698A1-20101021-C01113
         		6-chloro-2-{[2-(2,5- difluorophenyl)-1H-imidazol-1- yl]methyl}-1-ethyl-1H- benzimidazole 1H NMR (CDCl3): 7.67 (d, 1H), 7.37 (m, 1H), 7.17-7.30 (m, 5H), 7.01 (d, 1H), 5.35 (s, 2H), 3.79 (q, 2H), 0.99 (t, 3H)
    464
    Figure US20100267698A1-20101021-C01114
         		6-chloro-1-ethyl-2-{[2-(3- fluorophenyl)-1H-imidazol-1- yl]methyl}-1H-benzimidazole 1H NMR (CDCl3): 7.69 (d, 1H), 7.39-7.51 (m, 3H), 7.16-7.30 (m, 4H), 7.03 (d, 1H), 5.49 (s, 2H), 3.75 (q, 2H), 0.98 (t, 3H)
    465
    Figure US20100267698A1-20101021-C01115
         		2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-3-methyl- 3H-imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.40 (dd, 1H), 8.05 (dd, 1H), 7.40-7.51 (m, 3H), 7.13-7.29 (m, 3H), 7.02 (d, 1H), 5.53 (s, 2H), 3.51 (s, 3H)
    466
    Figure US20100267698A1-20101021-C01116
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-3-methyl- 3H-imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.40 (dd, 1H), 8.03 (dd, 1H), 7.36 (m, 1H), 7.15- 7.27 (m, 4H), 7.03 (d, 1H), 5.41 (s, 2H), 3.51 (s, 3H)
    467
    Figure US20100267698A1-20101021-C01117
         		2-{[2-(3-chloro-4-fluorophenyl)- 1H-imidazol-1-yl]methyl}-3- methyl-3H-imidazo[4,5-b]pyridine
    468
    Figure US20100267698A1-20101021-C01118
         		3-(cyclopropylmethyl)-2-{[2-(2,5- difluorophenyl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine 1H NMR (CDCl3): 8.37 (dd, 1H), 8.04 (dd, 1H), 7.38 (m, 1H), 7.09- 7.28 (m, 4H), 7.09 (d, 1H), 5.42 (s, 2H), 3.83 (d, 2H), 0.68 (m, 1H), 0.38-0.43 (m, 2H), 0.24-0.29 (m, 2H)
    469
    Figure US20100267698A1-20101021-C01119
         		6-chloro-2-{[2-(3-chlorophenyl)- 1H-imidazol-1-yl]methyl}-1- ethyl-1H-benzimidazole 1H NMR (CDCl3): 7.67-7.71 (m, 2H), 7.45-7.56 (m, 3H), 7.24-7.30 (m, 2H), 7.16 (d, 2H), 7.04 (d, 1H), 5.47 (s, 2H), 3.76 (q, 2H), 0.99 (t, 3H)
    470
    Figure US20100267698A1-20101021-C01120
         		2-{[2-(3-chlorophenyl)-1H- imidazol-1-yl]methyl}-3- (cyclopropylmethyl)-3H- imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.39 (dd, 1H), 8.06 (dd, 1H), 7.68 (m, 1H), 7.40- 7.68 (m, 3H), 7.27 (m, 1H), 7.18 (d, 1H), 7.10 (d, 1H), 5.53 (s, 2H), 3.83 (d, 2H), 0.73 (m, 1H), 0.40-0.46 (m, 2H), 0.24-0.28 (m, 2H)
    471
    Figure US20100267698A1-20101021-C01121
         		6-chloro-2-{[2-(3-chlorophenyl)- 1H-imidazol-1-yl]methyl}-1- (cyclopropylmethyl)-1H- benzimidazole 1H NMR (CDCl3): 7.67-7.71 (m, 2H), 7.42-7.56 (m, 3H), 7.24-7.33 (m, 2H), 7.15 (d, 1H), 7.06 (d, 1H), 5.47 (s, 2H), 3.58 (d, 2H), 0.69 (m, 1H), 0.47-0.53 (m, 2H), 0.03-0.09 (m, 2H)
    472
    Figure US20100267698A1-20101021-C01122
         		2-{[2-(3-chlorophenyl)-1H- imidazol-1-yl]methyl}-1-propyl- 1H-benzimidazole 1H NMR (CDCl3): 7.79 (m, 1H), 7.69 (m, 1H), 7.44-7.58 (m, 3H), 7.27-7.30 (m, 3H), 7.14 (d, 1H), 7.07 (d, 1H), 5.49 (s, 2H), 3.69 (t, 2H), 1.42 (m, 2H), 0.75 (t, 3H)
    473
    Figure US20100267698A1-20101021-C01123
         		2-{[2-(3-chlorophenyl)-1H- imidazol-1-yl]methyl}-3-ethyl- 3H-imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.40 (dd, 1H), 8.06 (dd, 1H), 7.67 (m, 1H), 7.43- 7.57 (m, 3H), 7.26 (m, 1H), 7.19 (d, 1H), 7.08 (d, 1H), 5.51 (s, 2H), 4.00 (q, 2H), 1.09 (t, 3H)
    474
    Figure US20100267698A1-20101021-C01124
         		2-{[2-(3-chlorophenyl)-1H- imidazol-1-yl]methyl}-3-propyl- 3H-imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.40 (dd, 1H), 8.06 (dd, 1H), 7.68 (m, 1H), 7.43- 7.56 (m, 3H), 7.26 (m, 1H), 7.18 (d, 1H), 7.09 (d, 1H), 5.50 (s, 2H), 3.89 (t, 2H), 1.52 (m, 2H), 0.79 (t, 3H)
    475
    Figure US20100267698A1-20101021-C01125
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1H- benzimidazole-5-carbonitrile 346.3 [M + 1], 344.2 [M − 1]
    476
    Figure US20100267698A1-20101021-C01126
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-5- (trifluoromethyl)-1H- benzimidazole 389.2 [M + 1]
    477
    Figure US20100267698A1-20101021-C01127
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl-5- (trifluoromethyl)-1H- benzimidazole 407.3 [M + 1]
    478
    Figure US20100267698A1-20101021-C01128
         		2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-3-(2,2,2- trifluoroethyl)-3H-imidazo[4,5- b]pyridine 376.2 [M + 1]
    479
    Figure US20100267698A1-20101021-C01129
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-3-(2,2,2- trifluoroethyl)-3H-imidazo[4,5- b]pyridine 394.2 [M + 1]
    480
    Figure US20100267698A1-20101021-C01130
         		2-{[2-(3-chlorophenyl)-1H- imidazol-1-yl]methyl}-3-(2,2,2- trifluoroethyl)-3H-imidazo[4,5- b]pyridine 392.2 [M + 1]
    481
    Figure US20100267698A1-20101021-C01131
         		1-ethyl-5-(trifluoromethyl)-2-({2- [3-(trifluoromethyl)phenyl]-1H- imidazol-1-yl}methyl)-1H- benzimidazole 1H NMR (CDCl3): 8.09 (d, 1H), 7.37-7.95 (m, 6H), 7.19 (d, 1H), 7.05 (d, 1H), 5.49 (s, 2H), 3.85 (q, 2H), 1.02 (t, 3H)
    482
    Figure US20100267698A1-20101021-C01132
         		5-chloro-3-propyl-2-({2-[3- (trifluoromethyl)phenyl]-1H- imidazol-1-yl}methyl)-3H- imidazo[4,5-b]pyridine 1H NMR (CDCl3): 7.60-7.97 (m, 5H), 7.22-7.27 (m, 2H), 7.09 (d, 1H), 5.45 (s, 2H), 3.85 (t, 2H), 1.51 (m, 2H), 0.76 (t, 3H)
    483
    Figure US20100267698A1-20101021-C01133
         		3-(2,2,2-trifluoroethyl)-2-({2-[3- (trifluoromethyl)phenyl]-1H- imidazol-1-yl}methyl)-3H- imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.44 (dd, 1H), 8.07 (dd, 1H), 7.54-7.89 (m, 4H), 7.24-7.34 (m, 2H), 7.12 (d, 1H), 5.51 (s, 2H), 4.51 (q, 2H)
    484
    Figure US20100267698A1-20101021-C01134
         		3-(cyclopropylmethyl)-2-({2-[3- (trifluoromethyl)phenyl]-1H- imidazol-1-yl}methyl)-3H- imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.39 (dd, 1H), 7.60-8.03 (m, 5H), 7.21-7.26 (m, 2H), 7.11 (d, 1H), 5.51 (s, 2H), 3.84 (d, 2H), 0.70 (m, 1H), 0.44 (m, 2H), 0.24 (m, 2H)
    485
    Figure US20100267698A1-20101021-C01135
         		3-isobutyl-2-({2-[3- (trifluoromethyl)phenyl]-1H- imidazol-1-yl}methyl)-3H- imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.39 (dd, 1H), 7.60-8.07 (m, 5H), 7.21-7.26 (m, 2H), 7.12 (d, 1H), 5.49 (s, 2H), 3.71 (d, 2H), 1.98 (m, 1H), 0.69 (d, 6H)
    486
    Figure US20100267698A1-20101021-C01136
         		3-propyl-2-({2-[3- (trifluoromethyl)phenyl]-1H- imidazol-1-yl}methyl)-3H- imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.40 (dd, 1H), 7.60-8.07 (m, 5H), 7.21-7.26 (m, 2H), 7.11 (d, 1H), 5.49 (s, 2H), 3.89 (t, 2H), 1.51 (m, 2H), 0.76 (t, 3H)
    487
    Figure US20100267698A1-20101021-C01137
         		1-propyl-2-({2-[3- (trifluoromethyl)phenyl]-1H- imidazol-1-yl}methyl)-1H- benzimidazole 1H NMR (CDCl3): 7.61-8.00 (m, 5H), 7.09-7.31 (m, 5H), 5.47 (s, 2H), 3.69 (t, 2H), 1.43 (m, 2H), 0.73 (t, 3H)
    488
    Figure US20100267698A1-20101021-C01138
         		6-chloro-1-(cyclopropylmethyl)- 2-({2-[3- (trifluoromethyl)phenyl]-1H- imidazol-1-yl}methyl)-1H- benzimidazole 1H NMR (CDCl3): 7.60-7.96 (m, 5H), 7.05-7.31 (m, 4H), 5.45 (s, 2H), 3.58 (d, 2H), 0.65 (m, 1H), 0.47 (m, 2H), 0.05 (m, 2H)
    489
    Figure US20100267698A1-20101021-C01139
         		3-ethyl-2-({2-[3- (trifluoromethyl)phenyl]-1H- imidazol-1-yl}methyl)-3H- imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.40 (dd, 1H), 7.60-8.06 (m, 5H), 7.21-7.28 (m, 2H), 7.09 (d, 1H), 5.49 (s, 2H), 4.01 (q, 2H), 1.09 (t, 3H)
    490
    Figure US20100267698A1-20101021-C01140
         		ethyl 2-{[2-(3-chlorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl- 1H-benzimidazole-5-carboxylate
    491
    Figure US20100267698A1-20101021-C01141
         		(2-{[2-(3-chlorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl- 1H-benzimidazol-5-yl)methanol
    492
    Figure US20100267698A1-20101021-C01142
         		2-{[2-(3-chlorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl-5- [(4-methylpiperidin-1-yl)methyl]- 1H-benzimidazole
    493
    Figure US20100267698A1-20101021-C01143
         		2-{[2-(3-chlorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl-5- (morpholin-4-ylmethyl)-1H- benzimidazole
    494
    Figure US20100267698A1-20101021-C01144
         		2-{[2-(3-chlorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl- 1H-benzimidazole-5-carboxylic acid
    495
    Figure US20100267698A1-20101021-C01145
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-5- (morpholin-4-ylmethyl)-1H- benzimidazole 420.4 [M + 1]; 418.2 [M − 1]
    496
    Figure US20100267698A1-20101021-C01146
         		2-{[2-(3-chlorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl- 1H-benzimidazole-5- carboxamide
    497
    Figure US20100267698A1-20101021-C01147
         		2-{[2-(3-chlorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl-N- methyl-1H-benzimidazole-5- carboxamide
    498
    Figure US20100267698A1-20101021-C01148
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1H- benzimidazole-5-carboxylic acid 365.1 [M + 1]
    499
    Figure US20100267698A1-20101021-C01149
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1H- benzimidazole-5-carboxamide
    500
    Figure US20100267698A1-20101021-C01150
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl- 1H-benzimidazole-5-carboxylic acid 383.2 [M + 1]
    501
    Figure US20100267698A1-20101021-C01151
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-5-[(4- methylpiperidin-1-yl)methyl]-1H- benzimidazole 432.4 [M + 1]
    502
    Figure US20100267698A1-20101021-C01152
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-N-[2- (dipropylamino)ethyl]-1-ethyl-1H- benzimidazole-5-carboxamide 509.5 [M + 1]
    503
    Figure US20100267698A1-20101021-C01153
         		5-fluoro-2-{[2-(4-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1- propyl-1H-benzimidazole 1H NMR(CDCl3): 7.65-7.71 (m, 2H), 7.47 (d, 1H), 7.03-7.23 (m, 6H), 5.47 (s, 2H), 3.65 (t, 2H), 1.41 (h, 2H), 0.71 (t, 3H)
    504
    Figure US20100267698A1-20101021-C01154
         		2-{[2-(3-chlorophenyl)-1H- imidazol-1-yl]methyl}-5-fluoro- 1-propyl-1H-benzimidazole 1H NMR(CDCl3): 7.65 (s, 1H), 7.41-7.53 (m, 3H), 7.06-7.24 (m, 5H), 5.47 (s, 2H), 3.65 (t, 2H), 1.47 (h, 2H), 0.71 (t, 3H)
    505
    Figure US20100267698A1-20101021-C01155
         		5-fluoro-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1- propyl-1H-benzimidazole 1H NMR(CDCl3): 7.35-7.47 (m, 4H), 7.0-7.1 (m, 5H), 5.47 (s, 2H), 3.65 (t, 2H), 1.35 (h, 2H), 0.71 (t, 3H)
    506
    Figure US20100267698A1-20101021-C01156
         		5-chloro-2-{[2-(2,5- difluorophenyl)-1H-imidazol-1- yl]methyl}-1-ethyl-1H- benzimidazole 1H NMR (CDCl3): 7.75 (d, 1H), 7.37 (m, 1H), 7.20-7.27 (m, 5H), 7.02 (d, 1H), 5.36 (s, 2H), [0.99 T, 3H), 3.82 (q, 2H)
    507
    Figure US20100267698A1-20101021-C01157
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl-5- fluoro-1H-benzimidazole 1H NMR (CDCl3): 7.43 (m, 1H), 7.37 (m, 1H), 7.19-7.25 (m, 4H), 7.03-7.10 (m, 2H), 5.35 (s, 2H), 3.82 (q, 2H), 1.00 (t, 3H)
    508
    Figure US20100267698A1-20101021-C01158
         		5-chloro-2-{[2-(3-chlorophenyl)- 1H-imidazol-1-yl]methyl}-1- ethyl-1H-benzimidazole (L)-Tartrate salt 1H NMR (CD3OD): 7.24-7.59 (m, 9H), 5.66 (s, 2H), 4.51 (s, 2H), 4.12 (q, 2H), 1.28 (t, 3H)
    509
    Figure US20100267698A1-20101021-C01159
         		2-{[2-(3-chlorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl- 1H-benzimidazole-5-carbonitrile 8.00 (s, 1H), 7.38-7.72 (m, 7H), 7.24 (s, 1H), 5.71 (s, 2H), 4.50 (s, 2H), 4.20 (q, 2H), 1.22 (t, 3H)
    510
    Figure US20100267698A1-20101021-C01160
         		2-{[2-(3-chlorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl-5- fluoro-1H-benzimidazole (L)-Tartrate salt 1H NMR (CD3OD): 7.08-7.60 (m, 9H), 5.66 (s, 2H), 4.51 (s, 2H), 4.12 (s, 2H), 1.18 (t, 3H)
    511
    Figure US20100267698A1-20101021-C01161
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl-5- (4H-1,2,4-triazol-4-yl)-1H- benzimidazole Hydrochloride 1H NMR (d6 DMSO): 9.57(2H, s), 8.11(1H, m), 8.01-7.99(1H, m), 7.86(1H, d), 7.78-7.74(1H, m), 7.66- 7.52(3H, m), 5.95(2H, s), 4.31(2H, q), 1.26(3H, t) 406.5 [M + 1]
    512
    Figure US20100267698A1-20101021-C01162
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl- 1H-benzimidazol-5-amine 354.4 [M + 1]
    513
    Figure US20100267698A1-20101021-C01163
         		1-ethyl-5-fluoro-2-{[2-(2- fluorophenyl)-1H-imidazol-1- yl]methyl}-1H-benzimidazole (L)-Tartrate salt 1H NMR (CD3OD): 7.07-7.58 (m, 9H), 5.53 (s, 2H), 4.51 (s, 2H), 4.03 (q, 2H), 1.07 (t, 3H)
    514
    Figure US20100267698A1-20101021-C01164
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1H- benzimidazol-5-amine 1H NMR(CDCl3): 7.38-7.5 (m, 4h), 7.15 (t, 3H), 7.1 (s, 1H), 7.05 (t, 2H), 6.7 (d, 1H), 5.4 (s, 2H), 3.70 (q, 2H), 0.9 (t, 3H)
    515
    Figure US20100267698A1-20101021-C01165
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-5- (4H-1,2,4-triazol-4-yl)- 1H-benzimidazole 388.2 [M + 1]
    516
    Figure US20100267698A1-20101021-C01166
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-5- (2H-tetraazol-5-yl)-1H- benzimidazole 389.2 [M + 1]
    517
    Figure US20100267698A1-20101021-C01167
         		5-bromo-1-ethyl-2-{[2-(3- fluorophenyl)-1H-imidazol-1- yl]methyl}-1H-benzimidazole 1H NMR (CDCl3): 7.93 (d, 1H), 7.39-7.52 (m, 4H), 7.15-7.22 (m, 3H), 7.025 (d, 1H), 5.49 (s, 2H), 3.76 (q, 2H), 0.97 (t, 3H)
    518
    Figure US20100267698A1-20101021-C01168
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl- 1H-benzimidazole-5- carbonitrile 1H NMR (CDCl3): 8.09 (d, 1H), 7.56 (m, 1H), 7.33-7.40 (m, 2H), 7.16-7.24 (m, 3H), 7.04 (d, 1H), 5.41 (s, 2H), 3.89 (q, 2H), 1.03 (t, 3H)
    519
    Figure US20100267698A1-20101021-C01169
         		2-{[2-(3-bromophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl- 5-fluoro-1H-benzimidazole Hydrochloride 1H NMR (d6- DMSO): 7.41-8.00 (m, 8H), 7.18 (m, 1H), 5.92 (s, 2H), 4.28 (q, 2H), 1.27 (t, 3H)
    520
    Figure US20100267698A1-20101021-C01170
         		2-{[2-(3-bromophenyl)-1H- imidazol-1-yl]methyl}-5-chloro- 1-ethyl-1H-benzimidazole Hydrochloride 1H NMR (d6- DMSO): 7.32-8.00 (m, 9H), 5.95 (s, 2H), 4.29 (q, 2H), 1.27 (t, 3H)
    521
    Figure US20100267698A1-20101021-C01171
         		3-{1-[(1-ethyl-5-fluoro-1H- benzimidazol-2-yl)methyl]-1H- imidazol-2-yl}benzonitrile 346.11 [M + 1]
    522
    Figure US20100267698A1-20101021-C01172
         		1-ethyl-5-fluoro-2-{[2-(3- methylphenyl)-1H-imidazol-1- yl]methyl}-1H-benzimidazole
    523
    Figure US20100267698A1-20101021-C01173
         		1-ethyl-5-fluoro-2-{[2-(3- methoxyphenyl)-1H-imidazol-1- yl]methyl}-1H-benzimidazole 351.15 [M + 1]
    524
    Figure US20100267698A1-20101021-C01174
         		4-{1-[(1-ethyl-5-fluoro-1H- benzimidazol-2-yl)methyl]-1H- imidazol-2-yl}-2-fluorophenol 355.12 [M + 1]
    525
    Figure US20100267698A1-20101021-C01175
         		2-{[2-(3-bromophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl- 1H-benzimidazole-5-carbonitrile 1H NMR (CDCl3): 8.11 (d, 1H), 7.81 (s, 1H), 7.55-7.67 (m, 3H), 7.35-7.41 (m, 2H), 7.19 (m, 1H), 7.04 (d, 1H), 5.51 (s, 2H), 3.84 (q, 2H), 1.04 (t, 3H)
    526
    Figure US20100267698A1-20101021-C01176
         		2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-1-propyl- 1H-benzimidazole-5-carbonitrile 360.2 [M + 1]; 358.2 [M − 1]
    527
    Figure US20100267698A1-20101021-C01177
         		2-{[2-(3-chlorophenyl)-1H- imidazol-1-yl]methyl}-1-propyl- 1H-benzimidazole-5-carbonitrile 376.2 [M + 1]; 374.2 [M − 1]
    528
    Figure US20100267698A1-20101021-C01178
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-propyl- 1H-benzimidazole-5-carbonitrile 378.2 [M + 1]
    529
    Figure US20100267698A1-20101021-C01179
         		2-{[2-(3-chloro-4-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1- propyl-1H-benzimidazole-5- carbonitrile 394.4 [M + 1]; 392.1 [M − 1]
    530
    Figure US20100267698A1-20101021-C01180
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl-5- (methylsulfonyl)-1H- benzimidazole
    531
    Figure US20100267698A1-20101021-C01181
         		1-ethyl-5-(3-fluorophenyl)-2-{[2- (3-fluorophenyl)-1H-imidazol-1- yl]methyl}-1H-benzimidazole 1H NMR (CDCl3): 7.98 (m, 1H), 7.27-7.54 (m, 8H), 7.16-7.22 (m, 2H), 7.01-7.07 (m, 2H), 5.53 (s, 2H), 3.82 (q, 2H), 1.02 (t, 3H)
    532
    Figure US20100267698A1-20101021-C01182
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-5- thien-3-yl-1H-benzimidazole 1H NMR (CDCl3): 8.0 (m, 1H), 7.16-7.59 (m, 10H), 7.07 (d, 1H), 5.52 (s, 2H), 3.81 (q, 2H), 1.01 (t, 3H)
    533
    Figure US20100267698A1-20101021-C01183
         		5-bromo-2-{[2-(3-chlorophenyl)- 1H-imidazol-2-yl]methyl}-1- ethyl-1H-benzimidazole 1H NMR (CDCl3): 7.93 (d, 1H), 7.68 (m, 1H), 7.40-7.57 (m, 2H), 7.15-7.19 (m, 2H), 7.03 (d, 1H), 5.48 (s, 2H), 3.77 (q, 2H), 0.98 (t, 3H)
    534
    Figure US20100267698A1-20101021-C01184
         		2-{[2-(3-cyanophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl- 1H-benzimidazole-5-carbonitrile 353.15 [M + 1]
    535
    Figure US20100267698A1-20101021-C01185
         		3-{1-[(1-ethyl-5-fluoro-1H- benzimidazol-2-yl)methyl]-1H- imidazol-2-yl}benzoic acid 364.10 [M + 1]
    536
    Figure US20100267698A1-20101021-C01186
         		1-ethyl-5-fluoro-2-{[2-(3-pyridin- 4-ylphenyl)-1H-imidazol-1- yl]methyl}-1H-benzimidazole 398.18 [M + 1]
    537
    Figure US20100267698A1-20101021-C01187
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl-5- nitro-1H-benzimidazole Hydrochloride 1H NMR (d6 DMSO): 8.42(1H, d), 8.17(1H, dd), 8.00(1H, s), 7.90(1H, s), 7.85(1H, d), 7.70(1H, m), 7.56- 7.51(2H, m), 5.90 (2H, s), 4.31(2H, q), 1.24(3H, t) 384.1 [M + 1]
    538
    Figure US20100267698A1-20101021-C01188
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-5- nitro-1H-benzimidazole 1H NMR (d6 DMSO): 8.47(1H, d), 8.19(1H, dd), 8.00(1H, m), 7.96(1H, m), 7.88(1H, d), 7.71- 7.50(4H, m), 5.98(2H, s), 4.35(2H, q), 1.30(3H, t) 366.2 [M + 1]
    539
    Figure US20100267698A1-20101021-C01189
         		2-{[2-(3-chlorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl-5- nitro-1H-benzimidazole 381.95 [M + 1]
    540
    Figure US20100267698A1-20101021-C01190
         		3-(1-{[1-ethyl-5-(methylsulfonyl)- 1H-benzimidazol-2-yl]methyl}- 1H-imidazol-2-yl)benzonitrile 406.3  [M + 1]; 404.3  [M − 1]
    541
    Figure US20100267698A1-20101021-C01191
         		3-{1-[(1-ethyl-5-fluoro-1H- benzimidazol-2-yl)methyl]-1H- imidazol-2-yl}phenol 337.01 [M + 1]
    542
    Figure US20100267698A1-20101021-C01192
         		2-{[2-(3,4-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl- 1H-benzimidazole-5- carbonitrile 379.11 [M + 1]
    543
    Figure US20100267698A1-20101021-C01193
         		2-{[2-(5-bromo-2-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1- ethyl-1H-benzimidazole-5- carbonitrile 425.98 [M + 1]
    544
    Figure US20100267698A1-20101021-C01194
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl- 5-(trifluoromethoxy)-1H- benzimidazole 1H NMR (CDCl3): 7.65 (s, 1H), 7.18-7.41 (m, 6H), 7.06 (s, 1H), 5.35 (s, 2H), 3.82 (q, 2H), 1.0 (t, 3H)
    545
    Figure US20100267698A1-20101021-C01195
         		5-chloro-1-ethyl-2-{[2-(3- fluorophenyl)-1H-imidazol-1- yl]methyl}-1H-benzimidazole 1H NMR (CDCl3): 7.22-7.60 (m, 9H), 5.67 (s, 2H), 4.49 (s, 2H), 4.11 (q, 2H), 1.16 (t, 3H)
    546
    Figure US20100267698A1-20101021-C01196
         		3-{1-[(5-bromo-1-ethyl-1H- benzimidazol-2-yl)methyl]-1H- imidazol-2-yl}benzonitrile Hydrochloride 1H NMR (CD3OD): 7.71-8.25 (m, 9H), 6.18 (s, 2H), 4.50 (br s, 2H), 1.50 (br s, 3H)
    547
    Figure US20100267698A1-20101021-C01197
         		1-ethyl-2-{[2-(3-nitrophenyl)-1H- imidazol-1-yl]methyl}-1H- benzimidazole-5-carbonitrile 373.15 [M + 1]
    548
    Figure US20100267698A1-20101021-C01198
         		6-chloro-1-ethyl-2-{[2-(3- fluorophenyl)-1H-imidazol-1- yl]methyl}-1H-benzimidazole-5- carbonitrile 380.4  [M + 1]; 378.2  [M − 1]
    549
    Figure US20100267698A1-20101021-C01199
         		5-(3,5-difluorophenyl)-1-ethyl-2- {[2-(3-fluorophenyl)-1H-imidazol- 1-yl]methyl}-1H-benzimidazole 1H NMR (CDCl3): 7.96 (d, 1H), 7.35-7.70 (m, 6H), 7.06-7.23 (m, 4H), 6.78 (m, 1H), 5.52 (s, 2H), 3.81 (q, 2H), 1.00 (t, 3H)
    550
    Figure US20100267698A1-20101021-C01200
         		3-(1-{[5-(3,5-difluorophenyl)-1- ethyl-1H-benzimidazol-2- yl]methyl}-1H-imidazol-2- yl)benzonitrile 1H NMR (CDCl3): 7.93-8.02 (m, 2H), 7.37-7.77 (m, 6H), 7.08-7.19 (m, 3H), 6.77 (m, 1H), 5.49 (s, 2H), 3.89 (q, 2H), 1.10 (t, 3H)
    551
    Figure US20100267698A1-20101021-C01201
         		1-(1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1H- benzimidazol-5-yl)ethanone 1H NMR (CDCl3): 8.40 (s, 1H), 8.00 (dd, 1H), 7.33-7.53 (m, 4H), 7.17-7.22 (m, 2H), 7.05 (d, 1H), 5.53 (s, 2H), 3.82 (q, 2H), 2.67 (s, 3H), 1.01 (t, 3H)
    552
    Figure US20100267698A1-20101021-C01202
         		5-(3,5-dimethylisoxazol-4-yl)-1- ethyl-2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-1H- benzimidazole 1H NMR (CDCl3): 7.66 (d, 1H), 7.34-7.54 (m, 4H), 7.15-7.22 (m, 3H), 7.06 (d, 1H), 5.52 (s, 2H), 3.83 (q, 2H), 2.41 (s, 3H), 2.28 (s, 3H), 1.02 (s, 3H)
    553
    Figure US20100267698A1-20101021-C01203
         		1-ethyl-2-({2-[3- (trifluoromethoxy)phenyl]-1H- imidazol-1-yl}methyl)-1H- benzimidazole-5-carbonitrile 411.96 [M + 1]
    554
    Figure US20100267698A1-20101021-C01204
         		(1E)-1-(1-ethyl-2-{[2-(3- fluorophenyl)-1H-imidazol-1- yl]methyl}-1H-benzimidazol-5- yl)ethanone O-methyloxime Syn and anti 1H NMR (CDCl3): 7.98 (d, 1H), 7.94 (d, 1H1), 7.75 (dd, 1H + H1), 7.14-7.22 (m, 2H + H1), 7.32-7.52 (m, 4H + H1), [7.03 (d, 1H), 7.01 (d, 1H1)], 5.49 (s, 2H + H1), [4.00 (s, 2H), 3.86 (s, 2H1)], 3.79 (q, 2H + H1), [2.30 (s, 3H), 2.26 (s, 3H1)], 0.98 (t, 3H + H1)
    555
    Figure US20100267698A1-20101021-C01205
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl- 5-[5-(trifluoromethyl)-1H- tetraazol-1-yl]-1H- benzimidazole 475.1  [M + 1]
    556
    Figure US20100267698A1-20101021-C01206
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1H- benzimidazole-5,6-dicarbonitrile 371.0  [M + 1]
    557
    Figure US20100267698A1-20101021-C01207
         		1-ethyl-2-[(2-pyridin-3-yl-1H- imidazol-1-yl)methyl]-1H- benzimidazole-5-carbonitrile Dihydrochloride 1H NMR (d6 DMSO): 8.96(1H, s), 8.82 (1H, d), 8.24(1H, d), 8.14(1H, s), 8.05(1H, d), 7.98(1H, d), 7.85(1H, d), 7.69-7.65(2H, m), 6.01(2H, s), 4.33(2H, q), 1.29(3H, t)
    558
    Figure US20100267698A1-20101021-C01208
         		1-ethyl-2-[(2-pyridin-2-yl-1H- imidazol-1-yl)methyl]-1H- benzimidazole-5-carbonitrile Dihydrochloride 1H NMR (d6 DMSO): 8.55(1H, d), 8.41 (1H, d), 8.10-7.98(4H, m), 7.85(1H, d), 7.64(1H, d), 7.54-7.51(1H, m), 6.39 (2H, s), 4.45(2H, q), 1.41(3H, t) 329.4  [M + 1]
    559
    Figure US20100267698A1-20101021-C01209
         		1-ethyl-5-fluoro-2-{[2-(3- nitrophenyl)-1H-imidazol-1- yl]methyl}-1H-benzimidazole 365.99 [M + 1]
    560
    Figure US20100267698A1-20101021-C01210
         		1-ethyl-2-{[2-(3-methylphenyl)- 1H-imidazol-1-yl]methyl}-1H- benzimidazole-5-carbonitrile 343.06 [M + 1]
    561
    Figure US20100267698A1-20101021-C01211
         		1-(3-{1-[(1-ethyl-5-fluoro-1H- benzimidazol-2-yl)methyl]-1H- imidazol-2-yl}phenyl)ethanone 363.05 [M + 1]
    562
    Figure US20100267698A1-20101021-C01212
         		3-(1-{[5-(3,5-dimethylisoxazol-4- yl)-1-ethyl-1H-benzimidazol-2- yl]methyl}-1H-imidazol-2- yl)benzonitrile 1H NMR (CDCl3): 7.94-8.01 (m, 2H), 7.75 (m, 1H), 7.61-7.66 (m, 2H), 7.38 (dd, 1H), 7.18-7.22 (m, 2H), 7.10 (d, 1H), 5.49 (s, 2H), 3.90 (q, 2H), 2.42 (s, 3H), 2.29 (s, 3H), 1.12 (t, 3H)
    563
    Figure US20100267698A1-20101021-C01213
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl-5- (1H-imidazol-2-yl)-1H- benzimidazole Dihydrochloride 1H NMR (d6 DMSO): 8.43(1H, s), 8.13- 8.11(2H, m), 7.99(1H, d), 7.89(1H, d), 7.79-7.75(3H, m), 7.60-7.51(3H, m), 5.95(2H, s), 4.31(2H, q), 1.26 (3H, t) 405.3  [M + 1]
    564
    Figure US20100267698A1-20101021-C01214
         		1-ethyl-5-nitro-2-[(2-pyridin-2-yl- 1H-imidazol-1-yl)methyl]-1H- benzimidazole 349.1  [M + 1]
    565
    Figure US20100267698A1-20101021-C01215
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl-5- (1-ethyl-1H-imidazol-2-yl)-1H- benzimidazole 433.2  [M + 1]
    566
    Figure US20100267698A1-20101021-C01216
         		3-{1-[(5-acetyl-1-ethyl-1H- benzimidazol-2-yl)methyl]-1H- imidazol-2-yl}benzonitrile 1H NMR (CDCl3): 8.40 (d, 1H), 7.93-8.05 (m, 3H), 7.76 (m, 1H), 7.63 (m, 1H), 7.37 (dd, 1H), 7.21 (d, 1H), 7.09 (d, 1H), 5.50 (s, 2H), 3.91 (q, 2H), 2.68 (s, 3H), 1.11 (t, 3H)
    567
    Figure US20100267698A1-20101021-C01217
         		1-ethyl-2-({2-[3- (trifluoromethyl)phenyl]-1H- imidazol-1-yl}methyl)-1H- benzimidazole-5-carbonitrile 396.06 [M + 1]
    568
    Figure US20100267698A1-20101021-C01218
         		1-ethyl-2-[(2-thien-2-yl-1H- imidazol-1-yl)methyl]-1H- benzimidazole-5-carbonitrile 334.04 [M + 1]
    569
    Figure US20100267698A1-20101021-C01219
         		3-(1-{[1-ethyl-5- (trifluoromethoxy)-1H- benzimidazol-2-yl]methyl}-1H- imidazol-2-yl)benzonitrile 412.2  [M + 1]; 410.2  [M − 1]
    570
    Figure US20100267698A1-20101021-C01220
         		3-{1-[(1-ethyl-5-fluoro-1H- benzimidazol-2-yl)methyl]-1H- imidazol-2-yl}benzaldehyde 349.17 [M + 1]
    571
    Figure US20100267698A1-20101021-C01221
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-N- methoxy-N-methyl-1H- benzimidazole-5-carboxamide 1H NMR (CDCl3): 8.19 (d, 1H), 7.71 (dd, 1H), 7.40-7.55 (m, 3H), 7.32 (dd, 1H), 7.16-7.23 (m, 2H), 7.05 (d, 1H), 5.53 (s, 2H), 3.80 (q, 2H), 3.59 (s, 3H), 3.41 (s, 3H), 1.00 (t, 3H)
    572
    Figure US20100267698A1-20101021-C01222
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-5- (1,3,4-oxadiazol-2-yl)-1H- benzimidazole
    573
    Figure US20100267698A1-20101021-C01223
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-5- (5-methyl-1,3,4-oxadiazol-2-yl)- 1H-benzimidazole Hydrochloride 1H NMR (d6 DMSO): 8.11 (1H, s), 8.00(1H, d), 7.95(1H, d), 7.90-7.82(2H, m), 7.74-7.72(1H, m), 7.64- 7.58(2H, m), 7.53-7.49(1H, m), 5.96(2H, s), 4.329(2H, q), 2.56(3H, s), 1.30(3H, t) 403.3  [M + 1]
    574
    Figure US20100267698A1-20101021-C01224
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-5- (5-methyl-1,2,4-oxadiazol-3-yl)- 1H-benzimidazole 403.8  [M + 1]; 401.4  [M − 1]
    575
    Figure US20100267698A1-20101021-C01225
         		1-ethyl-2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-5- [(trifluoromethyl)sulfonyl]-1H- benzimidazole 453.8  [M + 1]; 451.2  [M − 1]
    576
    Figure US20100267698A1-20101021-C01226
         		1-(2-{[2-(3-chlorophenyl)- 1H-imidazol-1-yl]methyl}-1-ethyl- 1H-benzimidazol-5-yl)ethanone 1H NMR (CDCl3): (8.40 (d, 1H), 8.01 (dd, 1H), 7.68 (m, 1H), 7.55 (m, 1H), 7.45-7.52 (m, 2H), 7.35 (d, 1H), 7.17 (d, 1H), 7.06 (d, 1H), 5.52 (s, 2H), 3.85 (q, 2H), 2.68 (s, 3H), 1.03 (t, 3H)
    577
    Figure US20100267698A1-20101021-C01227
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1H- benzimidazole-6-carbonitrile 346.2  [M + 1]; 344.3  [M − 1]
    578
    Figure US20100267698A1-20101021-C01228
         		methyl 3-{1-[(5-cyano-1-ethyl- 1H-benzimidazol-2-yl)methyl]- 1H-imidazol-2-yl}benzoate 386.17 [M + 1]
    579
    Figure US20100267698A1-20101021-C01229
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1H- imidazo[4,5-c]pyridine 1H NMR (CDCl3): 9.10 (s, 1H), 8.44 (d, 1H), 7.38-7.52 (m, 3H), 7.18-7.20 (m, 2H), 7.16 (s, 1H), 7.04 (d, 1H), 5.53 (s, 2H), 3.79 (q, 2H), 1.00 (t, 3H)
    580
    Figure US20100267698A1-20101021-C01230
         		379.05 [M + 1]
    581
    Figure US20100267698A1-20101021-C01231
         		2-{[2-(5-aminothien-3-yl)-1H- imidazol-1-yl]methyl}-1-ethyl- 1H-benzimidazole-5-carbonitrile 397.12 [M + 1]
    582
    Figure US20100267698A1-20101021-C01232
         		1-ethyl-2-{[2-(3-nitrophenyl)-1H- imidazol-1-yl]methyl}-1H- benzimidazole-5-carboxamide 391.17 [M + 1]
    583
    Figure US20100267698A1-20101021-C01233
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-5- (5-methyl-1,3-oxazole-2-yl)-1H- benzimidazole Hydrochloride 1H NMR (d6 DMSO): 8.05(1H, s), 7.99(1H, d), 7.97(1H, s), 7.88-7.86(1H, m), 7.76-7.72(2H, m), 7.65- 7.59(2H, m), 7.54-7.51(1H, m), 6.99(1H, s), 5.9392H, s), 4.31(2H, q), 2.36(3H, s), 1.29(3H, t). 402.5  [M + 1]
    584
    Figure US20100267698A1-20101021-C01234
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-5- (5-pyridin-3-yl-1,3,4-oxadiazol- 2-yl)-1H-benzimidazole Hydrochloride 1H NMR (d6 DMSO): 9.36(1H, d), 8.84(1H, dd), 8.57(1H, m), 8.44(1H, s), 8.10(1H, dd), 8.03(1H, d), 7.98(1H, d), 7.90(1H, d), 7.75- 7.51(5H, m), 5.99(2H, s), 4.35(2H, q), 1.33(3H, t). 466.5  [M + 1]
    585
    Figure US20100267698A1-20101021-C01235
         		(1E)-1-(2-{[2-(3-chlorophenyl)- 1H-imidazol-1-yl]methyl}-1- ethyl-1H-benzimidazol-5- yl)ethanone oxime 1H NMR (CDCl3): 11.03 (s, 1H), 7.81 (s, 1H), 7.52-7.67 (m, 4H), 7.40-7.45 (m, 2H), 7.31 (s, 1H), 7.07 (s, 1H), 5.67 (s, 2H), 4.17 (q, 2H), 2.18 (s, 3H), 1.16 (t, 3H)
    586
    Figure US20100267698A1-20101021-C01236
         		(1E)-1-(2-{[2-(3-chlorophenyl)- 1H-imidazol-1-yl]methyl}-1- ethyl-1H-benzimidazol-5- yl)ethanone O-methyloxime Syn and anti 1H NMR (CDCl3): 7.99 (dd, 1H), 7.95 (dd, 1H1), 7.76 (dd, 1H + H1), 7.65 (m, 1H + H1), 7.44-7.57 (m, 3H + H1), 7.27 (d, 1H + H1), 7.15 (d, 1H + H1), 7.04 (d, 1H + H1), 5.49 (s, 2H + H1), 4.01 (s, 3H), 3.87 (s, 3H1), 3.80 (q, 2H + H1), [2.30 (s, 3H), 2.27 (s, 3H1)], 1.00 (t, 3H + H1)
    587
    Figure US20100267698A1-20101021-C01237
         		(1E)-1-(2-{[2-(3-chlorophenyl)- 1H-imidazol-1-yl]methyl}-1- ethyl-1H-benzimidazol-5- yl)ethanone O-ethyloxime Syn and anti 1H NMR (CDCl3): 8.00 (dd, 1H + H1), 7.76 (dd, 1H + H1), 7.69 (m, 1H + H1), 7.44- 7.57 (m, 3H + H1), 7.27 (dd, 1H + H1), 7.15 (d, 1H + H1), 7.04 (d, 1H), 7.03 (d, 1H1), 5.49 (s, 2H + H1), 4.26 (q, 2H), 4.13 (q, 2H1), 3.80 (q, 2H + H1), 2.27 (s, 3H1), 1.34 (t, 3H), 1.25 (t, 3H1), 1.00 (t, 3H + H1)
    588
    Figure US20100267698A1-20101021-C01238
         		1-ethyl-2-{[2-(6-fluoropyridin-2- yl)-1H-imidazol-1-yl]methyl}- 1H-benzimidazole-5-carbonitrile 347.07 [M + 1]
    589
    Figure US20100267698A1-20101021-C01239
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-5- (1,2,4-oxadiazol-3-yl)-1H- benzimidazole
    590
    Figure US20100267698A1-20101021-C01240
         		1-ethyl-2-{[2-(6- methoxypyridin-2-yl)-1H- imidazol-1-yl]methyl}-1H- benzimidazole-5-carbonitrile 359.08 [M + 1]
    591
    Figure US20100267698A1-20101021-C01241
         		(1E)-1-(1-ethyl-2-{[2-(3- fluorophenyl)-1H-imidazol-1- yl]methyl}-1H-benzimidazol-5- yl)ethanone oxime Hydrochloride 1H NMR (d6- DMSO): 11.10 s, 1H), 7.52-7.99 (m, 9H + H1), 5.923 (s, 2H + H1): 4.30 (q, 2H + H1), 2.18 (s, 3H), 2.14 (s, 3H1), 1.29 (t, 3H + H1)
    592
    Figure US20100267698A1-20101021-C01242
         		(1E)-1-(1-ethyl-2-{[2-(3- fluorophenyl)-1H-imidazol-1- yl]methyl}-1H-benzimidazol-5- yl)ethanone O-ethyloxime Hydrochloride 1H NMR (d6- DMSO): 7.52-7.99 (m, 9H), 5.93 (s, 2H), 4.28 (q, 2H), 4.14 (q, 2H), 2.20 (s, 3H), 1.23-1.29 (m, 6H)
    593
    Figure US20100267698A1-20101021-C01243
         		1-(1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1H- benzimidazol-5-yl)propan-1-one 1H NMR (CDCl3): 8.42 (d, 1H), 8.02 (dd, 1H), 7.32-7.54 (m, 4H), 7.17-7.24 (m, 2H), 7.06 (d, 1H), 5.53 (s, 2H), 3.83 (q, 2H), 3.09 (q, 2H), 1.27 (t, 3H), 1.02 (t, 3H)
    594
    Figure US20100267698A1-20101021-C01244
         		ethyl 1-(1-ethyl-2-{[2-(3- fluorophenyl)-1H-imidazol-1- yl]methyl}-1H-benzimidazol-5- yl)-1H-1,2,3-triazole-4- carboxylate
    595
    Figure US20100267698A1-20101021-C01245
         		3-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-3H- imidazo[4,5-c]pyridine
    596
    Figure US20100267698A1-20101021-C01246
         		1-(1-ethyl-2-{[2-(6- fluoropyridin-2-yl)-1H-imidazol- 1-yl]methyl}-1H-benzimidazol- 5-yl)ethanone 364.2  [M + 1]; 362.6  [M − 1]
    597
    Figure US20100267698A1-20101021-C01247
         		1-ethyl-2-{[2-(6-fluoropyridin-2- yl)-1H-imidazol-1-yl]methyl}-5- (trifluoromethyl)-1H- benzimidazole 390.2  [M + 1]; 388.2  [M − 1]
    598
    Figure US20100267698A1-20101021-C01248
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-propyl- 1H-benzimidazole-5-carbonitrile 1H NMR (399.96 MHz, CDCl3): d 8.17 (dd, J = 2.0, 7.6 Hz, 1H), 8.05 (s, 1H), 7.88 (q, J = 8.0 Hz, 1H,), 7.52 (d, J = 8.4 Hz, 1H), 7.41(d, J = 8.4 Hz, 1H), 7.21 (s, 1H), 7.18 (s, 1H), 6.28 (s, 2H), 4.28 (t, J = 7.6 Hz, 2H), 1.68 (dt, J = 7.6 Hz, 2H), 0.84 (t, J = 7.6 Hz, 3H) 361.2  [M + 1]; 359.2  [M − 1]
    599
    Figure US20100267698A1-20101021-C01249
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1H- imidazo[4,5-b]pyridine 322.2 [M + 1]; 320.3  [M − 1]
    600
    Figure US20100267698A1-20101021-C01250
         		2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-1-propyl- 1H-imidazo[4,5-c]pyridine 1H NMR (CDCl3): 9.11 (s, 1H), 8.45 (d, 1H), 7.38-7.52 (m, 3H), 7.16-7.26 (m, 3H) 7.06 (s, 1H), 5.53 (s, 2H), 3.71 (t, 2H), 1.43 (q 2H), 0.75 (t, 3H)
    601
    Figure US20100267698A1-20101021-C01251
         		2-{[2-(3-chlorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl-5- isoxazol-5-yl-1H-benzimidazole 1H NMR (CDCl3): 8.30 (d, 1H), 8.21 (dd, 1H), 7.78 (dd, 1H), 7.69 (m, 1H), 7.56 (m, 1H), 7.45-7.48 (m, 2H), 7.39 (dd, 1H), 7.17 (d, 1H), 7.07 (d, 1H), 6.53 (d, 1HP, 5.52 (s, 2H), 3.83 (q, 2H), 1.03 (t, 3H)
    602
    Figure US20100267698A1-20101021-C01252
         		1-(2-fluoroethyl)-2-{[2-(3- fluorophenyl)-1H-imidazol-1- yl]methyl}-1H-benzimidazole 339.2  [M + 1]
    603
    Figure US20100267698A1-20101021-C01253
         		2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-1-propyl- 1H-imidazo[4,5-b]pyridine 336.2  [M + 1]; 334.2 [M − 1]
    604
    Figure US20100267698A1-20101021-C01254
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl- 1H-imidazo[4,5-b]pyridine
    605
    Figure US20100267698A1-20101021-C01255
         		1-ethyl-2-[(2-{5- [hydroxy(oxido)amino]thien-3- yl}-1H-imidazol-1-yl)methyl]- 1H-benzimidazole-5-carbonitrile
    606
    Figure US20100267698A1-20101021-C01256
         		3-propyl-2-[(2-pyrimidin-2-yl- 1H-imidazol-1-yl)methyl]-3H- imidazo[4,5-b]pyridine (CDCl3) 8.46 (d, 1H), 8.39 (dd, 1H), 8.03 (dd, 1H), 7.27 (m, 5H), 6.37 (s, 2H), 4.27 (t, 2H), 1.70 (p, 2H), 0.82 (t, 3H) 320.4  [M + 1]
    607
    Figure US20100267698A1-20101021-C01257
         		3-ethyl-2-{[2-(2,3,4- trifluorophenyl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine (CDCl3) 8.40 (dd, 1H), 8.02 (dd, 1H), 7.36 (dq, 1H), 7.25 (dd, 1H), 7.22 (d, 1H), 7.01 (m, 2H), 5.38 (s, 2H), 4.03 (q, 2H), 1.10 (t, 3H) 358.2  [M + 1]
    608
    Figure US20100267698A1-20101021-C01258
         		3-propyl-2-[(2-thien-2-yl-1H- imidazol-1-yl)methyl]-3H- imidazo[4,5-b]pyridine (CDCl3) 8.40 (dd, 1H), 8.05 (dd, 1H), 7.46 (dd, 1H), 7.25 (dd, 1H), 7.15 (m, 2H), 7.03 (d, 1H), 5.61 (s, 2H), 3.96 (t, 2H), 1.55 (p, 2H), 0.80 (t, 3H) 324.3  [M + 1]
    609
    Figure US20100267698A1-20101021-C01259
         		3-ethyl-2-{[2-(1-methyl-1H- pyrazol-3-yl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine (CDCl3) 8.39 (dd, 1H), 8.05 (dd, 1H), 7.43 (dd, 1H), 7.23 (dd, 1H), 7.10 (dd, 1H), 7.04 (dd, 1H), 6.91 (dd, 1H), 6.21 (s, 2H), 4.33 (q, 2H), 3.97 (3, 3H), 1.13 (t, 3H) m/z 308.3 [M + 1]
    610
    Figure US20100267698A1-20101021-C01260
         		3-ethyl-2-{[2-(2-fluoro-5- methylphenyl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine (CDCl3) 8.39 (dd, J = 4.8, 1.5 Hz, 1H), 8.05 (dd, J = 8.4, 1.5 Hz, 1H), 7.44 (dd, J = 6.6, 2.1 Hz, 1H), 7.25-7.30 (m, 2H), 7.19 (d, J = 1.5 Hz, 1H), 7.12 (dd, J = 9.9, 8.7 Hz, 1H), 7.04 (d, J = 1.5 Hz, 1H), 5.38 (s, 2H), 3.96 (q, J = 7.2 Hz, 2H), 2.37 (s, 3H), 1.03 (t, J = 7.2 Hz, 3H) 336.2  [M + 1]
    611
    Figure US20100267698A1-20101021-C01261
         		3-ethyl-2-{[2-(5-fluoro-2- methylphenyl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine (CDCl3) 8.39 (dd, J = 4.6, 1.5 Hz, 1H), 8.04 (dd, J = 8.0, 1.5 Hz, 1H), 7.19-7.32 (m, 3H), 7.07- 7.13 (m, 3H), 5.26 (s, 2H), 3.98 (q, J = 7.2 Hz, 2H), 2.24 (s, 3H), 1.11 (t, J = 7.2 Hz, 3H) m/z 336.2 [M + 1]
    612
    Figure US20100267698A1-20101021-C01262
         		2-{[2-(3-chloro-2,6- difluorophenyl)-1H-imidazol-1- yl]methyl}-3-ethyl-3H- imidazo[4,5-b]pyridine (CDCl3) 8.40 (dd, J = 4.7, 1.5 Hz, 1H), 8.01 (dd, J = 8.0, 1.5 Hz, 1H), 7.52 (m, 1H), 7.23-7.30 (m, 2H), 7.11 (d, J = 1.5 Hz, 1H), 7.03 (m, 1H), 5.34 (s, 2H), 4.03 (q, J = 7.2 Hz, 2H), 1.10 (t, J = 7.2 Hz, 3H) m/z 374.2 [M + 1]
    613
    Figure US20100267698A1-20101021-C01263
         		3-ethyl-2-{[2-(2-methoxyphenyl)- 1H-imidazol-1-yl]methyl}-3H- imidazo[4,5-b]pyridine (CDCl3) 8.38 (dd, J = 4.8, 1.5 Hz, 1H), 8.03 (dd, J = 8.1, 1.5 Hz, 1H), 7.46-7.57 (m, 2H), 7.25 (m, 1H), 7.16 (d, J = 1.2 Hz, 1H), 7.13 (dt, J = 7.5, 1.2 Hz, 1H), 7.04 (d, J = 8.4 Hz, 1H), 7.00 (d, J = 1.2 Hz, 1H), 5.29 (s, 2H), 3.90 (q, J = 7.2 Hz, 2H), 3.84 (s, 3H), 0.98 (t, J = 7.2 Hz, 3H) m/z 334.2 [M + 1]
    614
    Figure US20100267698A1-20101021-C01264
         		3-ethyl-2-{[2-(2,4,5- trifluorophenyl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine (CDCl3) 8.40 (dd, J = 4.6, 1.4 Hz, 1H), 8.04 (dd, J = 8.2, 1.5 Hz, 1H), 7.50 (m, 1H), 7.25 (dd, J = 7.8, 5.1 Hz, 1H), 7.21 (d, J = 1.5 Hz, 1H), 7.14 (m, 1H), 7.06 (d, J = 1.5 Hz, 1H), 5.37 (s, 2H), 4.02 (q, J = 7.2 Hz, 2H), 1.11 (t, J = 7.2 Hz, 3H) m/z 358.2 [M + 1]
    615
    Figure US20100267698A1-20101021-C01265
         		4-chloro-2-{[2-(6-fluoropyridin- 2-yl)-1H-imidazol-1-yl]methyl}- 6-methyl-1-propyl-1H- imidazo[4,5-c]pyridine (CDCl3) 8.19(dd, J = 6, 1.2 Hz, 1H), 7.93(q, J = 6 Hz, 1H), 7.21(d, J = 1 Hz, 1H), 7.14(d, J = 1 Hz, 1H), 7.05(s, 1H), 6.93(dd, J = 6, 2 Hz, 1H), 6.38(s, 2H), 4.18(t, J = 5.6 Hz, 2H), 2.63(s, 3H), 1.57(sextet, J = 5.4 Hz, 2H), 0.77(t, J = 5.4 Hz, 3H) m/z 385 [M + 1}
    616
    Figure US20100267698A1-20101021-C01266
         		4-chloro-6-methyl-1-propyl-2- [(2-pyridin-2-yl-1H-imidazol-1- yl)methyl]-1H-imidazo[4,5- c]pyridine (CD3OD) 8.02-7.98(m, 3H), 7.34(d, J = 2 Hz, 1H), 7.14(d, J = 1.8 Hz, 1H), 7.01(d, J = 2 Hz, 1H), 6.47(s, 1H), 6.12(s, 2H), 4.21(t, J = 5.2 Hz, 2H), 2.33(s, 3H), 1.68(sextet, J = 5.4 Hz, 2H), 0.86(t, J = 5.4 Hz, 3H) m/z 367 [M + 1]
    617
    Figure US20100267698A1-20101021-C01267
         		3-ethyl-2-{[2-(5-fluoro-2- methoxyphenyl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine (CDCl3) 8.39 (dd, J = 4.6, 1.4 Hz, 1H), 8.04 (dd, J = 8.0, 1.4 Hz, 1H), 7.15-7.32 (m, 4H), 7.01 (d, J = 1.5 Hz, 1H), 6.96 (dd, J = 9.0, 4.5 Hz, 1H), 5.30 (s, 2H), 3.95 (q, J = 7.2 Hz, 2H), 3.80 (s, 3H), 1.02 (t, J = 7.2 Hz, 3H) m/z 352.3 [M + 1]
    618
    Figure US20100267698A1-20101021-C01268
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-5-methyl- 3-propyl-3H-imidazo[4,5- b]pyridine (CDCl3) 8.18 (dd, J = 7.8, 2.1 Hz, 1H), 7.84-7.92 (m, 2H), 7.21 (d, J = 1.2 Hz, 1H), 7.18 (d, J = 1.2 Hz, 1H), 7.06 (d, J = 7.8 Hz, 1H), 6.89 (dd, J = 8.1, 2.7 Hz, 1H), 6.29 (s, 2H), 4.29 (t, J = 7.5 Hz, 2H), 2.64 (s, 3H), 1.72 (m, 2H), 0.83 (t, J = 7.5 Hz, 3H)
    619
    Figure US20100267698A1-20101021-C01269
         		3-ethyl-2-{[2-(2,3,5- trifluorophenyl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine (CDCl3) 8.40 (dd, J = 4.8, 1.2 Hz, 1H), 8.04 (dd, J = 7.8, 1.2 Hz, 1H), 7.05-7.28 (m, 5H), 5.40 (s, 2H), 4.04 (q, J = 7.3 Hz, 2H), 1.10 (t, J = 7.3 Hz, 3H)
    620
    Figure US20100267698A1-20101021-C01270
         		2-{[2-(1H-benzimidazol-5-yl)-1H- imidazol-1-yl]methyl}-3-propyl- 3H-imidazo[4,5-b]pyridine (CDCl3): 8.38 (d, 1H), 8.02 (m, 2H), 7.96 (s, 1H), 7.66 (s, 1H), 7.42 (d, 1H), 7.25 (m, 2H), 5.56 (s, 2H), 5.29 (1H), 3.87 (t, 2H), 1.48 (m, 2H), 0.68 (t, 3H) m/z 358 [M + 1]
    621
    Figure US20100267698A1-20101021-C01271
         		3-{1-[(3-ethyl-3H-imidazo[4,5- b]pyridin-2-yl)methyl]-1H- imidazol-2-yl}-4- fluorobenzonitrile (CDCl3): 8.40 (d, 1H), 7.96 (m, 2H), 7.75 (m, 1H), 7.31 (m, 3H), 7.10 (s, 1H), 5.38 (s, 2H), 4.03 (q, 2H), 1.11 (t, 3H)
    622
    Figure US20100267698A1-20101021-C01272
         		2-{[2-(2,6-difluoro-3- methylphenyl)-1H-imidazol-1- yl]methyl}-1-ethyl-1H- benzimidazole-5-carbonitrile (CDCl3) 8.06 (s, 1H), 7.55 (m, 1H), 7.37 (dd, J = 8.0, 1.2 Hz, 1H), 7.32-7.26 (m, 2H), 7.04 (s, 1H), 6.94 (t, J = 8.8 Hz, 1H), 5.34 (s, 2H), 3.88 (q, J = 7.1 Hz, 2H), 2.25 (S, 3H), 1.03 (t, J = 7.1 Hz, 3H) m/z 378 [M + 1]
    623
    Figure US20100267698A1-20101021-C01273
         		1-{1-[(3-ethyl-3H-imidazo[4,5- b]pyridin-2-yl)methyl]-1H- imidazol-2-yl}isoquinoline (CDCl3) 9.11 (d, 1H), 8.58 (d, 1H), 8.37 (dd, 1H), 8.02 (dd, 1H), 7.86 (dd, 1H), 7.51 (m, 3H), 7.32 (d, 1H), 7.22 (m, 2H), 6.11 (s, 2H), 4.36 (q, 2H), 1.11 (t, 3H) m/z 355.2 [M + 1]
    624
    Figure US20100267698A1-20101021-C01274
         		2-{[2-(2-fluoro-5- methylphenyl)-1H-imidazol-1- yl]methyl}-1-propyl-1H- imidazo[4,5-c]pyridine (CDCl3) 9.07(s, 1H), 8.41(d, J = 4.5 Hz, 1H), 8.12(dd, J = 5.1, 1.5 Hz, 1H), 7.28-7.24(m, 2H), 7.21(d, J = 4.2 Hz, 1H), 7.10(t, J = 6.6 Hz, 1H), 7.01(s, 1H), 5.37(s, 2H), 3.69(t, J = 5.7 Hz, 2H), 2.34(s, 3H), 1.40(sextet, J = 5.7 Hz, 2H), 0.74(t, J = 5.7 Hz, 3H) m/z 350 [M + 1]
    625
    Figure US20100267698A1-20101021-C01275
         		1-ethyl-2-{[2-(2-fluoro-5- methylphenyl)-1H-imidazol-1- yl]methyl}-1H-imidazo[4,5- c]pyridine (CDCl3) 9.09(s, 1H), 8.44(d, J = 4.8 Hz, 1H), 7.42(d, J = 5.0 Hz, 1H), 7.29-7.23(m, 3H), 7.12(t, J = 6.3 Hz, 1H), 7.00(s, 1H), 5.39(s, 2H), 3.81(q, J = 5.4 Hz, 2H), 2.36(s, 3H), 0.99(t, J = 5.1 Hz, 3H) m/z 336 [M + 1]
    626
    Figure US20100267698A1-20101021-C01276
         		2-{[2-(3-chloro-2,6- difluorophenyl)-1H-imidazol-1- yl]methyl}-1-ethyl-1H- imidazo[4,5-c]pyridine D6dmso) 9.42 (s, 1H), 8.69 (d, J = 6.4 Hz, 1H), 8.36 (d, J = 6.4 Hz, 1H), 7.99-7.91 (m, 2H), 7.75 (s, 1H), 7.39 (t, J = 8 Hz, 1H), 5.95 (s, 2H), 4.43 (q, J = 7.2 Hz, 2H), 1.25 (t, J = 7.2 Hz, 3H) m/z 374 [M + 1]
    627
    Figure US20100267698A1-20101021-C01277
         		1-ethyl-2-(6-{1-[(1-ethyl-1H- imidazo[4,5-c]pyridin-2- yl)methyl]-1H-imidazol-2- yl}pyridin-2-yl)-1H- imidazo[4,5-c]pyridine (D6dmso) 9.57 (s, 1H), 9.34 (s, 1H), 8.74 (d, J = 6.8 Hz, 1H), 8.63 (d, J = 6.4 Hz, 1H), 8.42 (d, J = 6.8 Hz, 1H), 8.33 (d, J = 6.4 Hz, 1H), 8.25 (t, J = 8 Hz, 1H), 8.17 (d, J = 6.8 Hz, 1H), 8.07 (d, J = 7.6 Hz, 1H), 7.70 (s, 1H), 7.46 (s, 1H), 6.32 (s, 2H), 4.24-4.16 (m, 4H), 0.97 (t, J = 7.2 Hz, 3H), 0.89 (t, J = 7.2 Hz, 3H)
    628
    Figure US20100267698A1-20101021-C01278
         		2-{[2-(2,6-difluoro-3- methylphenyl)-1H-imidazol-1- yl]methyl}-1-ethyl-1H- imidazo[4,5-c]pyridine (d6 DMSO) 9.42 (s, 1H), 8.69 (d, J = 6.4 Hz, 1H), 8.37 (d, J = 6.8 Hz, 1H), 8.08 (s, 1H), 7.90 (s, 1H), 7.67-7.61 (m, 1H), 7.24 (t, J = 8.8 Hz, 1H), 5.98 (s, 2H), 4.44 (q, J = 7.2 Hz, 2H), 2.19 (s, 3H), 1.27 (t, J = 7.2 Hz, 3H)
    629
    Figure US20100267698A1-20101021-C01279
         		3-{1-[(3-ethyl-3H-imidazo[4,5- b]pyridin-2-yl)methyl]-1H- imidazol-2-yl}pyridin-2(1H)-one (d6 DMSO) 12.00 (br s, 1H), 8.28 (dd, J = 5.2, 1.2 Hz, 1H), 7.93 (dd, J = 8, 1.2 Hz, 1H), 7.56 (dd, J = 6.8, 2 Hz, 1H), 7.45 (dd, J = 6.8, 2 Hz, 1H), 7.25 (s, 1H), 7.20 (dd, J = 8, 4.8 Hz, 1H), 6.98 (s, 1H), 6.19 (t, J = 5.2 Hz, 1H), 5.64 (s, 2H), 4.14 (q, J = 7.2 Hz, 2H), 1.07 (t, J = 7.2 Hz, 3H) m/z 319 [M − H]
    630
    Figure US20100267698A1-20101021-C01280
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-3,5- dimethyl-3H-imidazo[4,5- b]pyridine (CDCl3) 8.16 (dd, 1H), 7.88(m, 2H), 7.16 (m, 2H), 7.06 (d, 1H), 6.85 (dd, 1H), 6.24 (s, 2H), 3.85 (s, 3H), 2.62 (s, 3H)
    631
    Figure US20100267698A1-20101021-C01281
         		8-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-2,9- dimethyl-9H-purine (CDCl3) 8.92 (s, 1H), 8.18 (dd, 1H), 7.88 (q, 1H), 7.25 (s, 1H), 7.20 (s, 1H), 7.11 (s, 1H), 6.83 (dd, 1H), 6.22 (s, 2H), 3.89 (s, 3H), 2.8 (s, 3H) m/z 324 [M + 1]
    632
    Figure US20100267698A1-20101021-C01282
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1,6- dimethyl-1H-imidazo[4,5- c]pyridine (CDCl3) 8.91 (s, 1H), 8.16 (dd, J = 7.6, 2.0 Hz, 1H), 7.85 (q, J = 8.0 Hz, 1H), 7.20 (s, 1H), 7.18 (s, 1H), 7.11 (s, 1H), 6.84 (dd, J = 8.0, 2.8 Hz, 1H), 6.22 (s, 2H), 3.80 (s, 3H), 2.66 (s, 3H) m/z 323 [M + 1]
    633
    Figure US20100267698A1-20101021-C01283
         		6-chloro-1-ethyl-2-{[2-(6- fluoropyridin-2-yl)-1H-imidazol- 1-yl]methyl}-1H-benzimidazole (CDCl3) 8.17 (dd, 1H), 7.89 (q, 1H), 7.25 (s, 1H), 7.75 (s, 1H), 7.26 (m, 2H), 7.19 (s, 1H), 7.17 (s, 1H), 6.89 (dd, 1H), 6.28 (s, 2H), 4.33 (q, 2H), 1.20 (s, 3H) m/z 356 [M + 1]
    634
    Figure US20100267698A1-20101021-C01284
         		1-ethyl-2-{[2-(6-fluoropyridin-2- yl)-1H-imidazol-1-yl]methyl}-6- (trifluoromethyl)-1H- benzimidazole (CDCl3) 8.20 (m, 1H), 7.87(m, 2H), 7.63 (s, 1H), 7.48 (d, 1H), 7.20 (d, 2H), 6.85 (dd, 1H), 6.25 (s, 2H), 4.38 (q, 2H), 1.24 (t, 3H)
    635
    Figure US20100267698A1-20101021-C01285
         		1-ethyl-2-{[2-(3-fluoropyridin-2- yl)-1H-imidazol-1-yl]methyl}-6- (trifluoromethyl)-1H- benzimidazole (CDCl3) 8.46 (m, 1H), 7.84(d, 1H), 7.60 (m, 2H), 7.52 (d, 1H), 7.38 (m, 1H), 7.25 (s, 1H), 7.10 (s, 1H), 6.14 (s, 2H), 4.35 (q, 2H), 1.14 (t, 3H)
    636
    Figure US20100267698A1-20101021-C01286
         		1-ethyl-2-{[2-(3-fluoropyridin-2- yl)-1H-imidazol-1-yl]methyl}-6- methyl-1H-benzimidazole (CDCl3) 8.48 (dt, 1H), 7.59-7.66 (m, 2H), 7.36 (m, 1H), 7.20 (s, 1H), 7.08-7.11 (m, 3H), 6.01 (s, 2H), 4.18 (q, 2H), 2.48 (s, 3H), 1.07 (s, 3H)
    637
    Figure US20100267698A1-20101021-C01287
         		6-chloro-1-ethyl-2-{[2-(3- fluoropyridin-2-yl)-1H-imidazol-1- yl]methyl}-1H-benzimidazole (CDCl3) 8.48 (dt, 1H), 7.74 (s, 1H), 7.61 (t, 1H), 7.37 (m, 1H), 7.20 (s, 1H), 7.23-7.26 (m, 3H), 7.08 (s, 1H), 6.01 (s, 2H), 4.25 (q, 2H), 1.07 (s, 3H)
    638
    Figure US20100267698A1-20101021-C01288
         		2-{[2-(3-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1,6- dimethyl-1H-imidazo[4,5- c]pyridine (CDCl3) 8.95 (s, 1H), 8.45 (dt, J = 5.8, 1.8 Hz, 1H), 7.61 (m, 1H), 7.36 (m, 1H), 7.27 (s, 1H), 7.10 (s, 1H), 7.06 (s, 1H), 6.06 (s, 2H), 3.66 (s, 3H), 2.67 (s, 3H) m/z 323 [M + 1]
    639
    Figure US20100267698A1-20101021-C01289
         		2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-1H- benzimidazole m/z 293 [M + 1]
    640
    Figure US20100267698A1-20101021-C01290
         		1-benzyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1H- benzimidazole m/z 383 [M + 1]
    641
    Figure US20100267698A1-20101021-C01291
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-methyl- 6-(trifluoromethyl)-1H- benzimidazole (CDCl3) 8.20 (m, 1H), 7.87(m, 2H), 7.63 (s, 1H), 7.48 (d, 1H), 7.20 (s, 2H), 6.84 (dd, 1H), 6.25 (s, 2H), 3.92 (s, 3H)
    642
    Figure US20100267698A1-20101021-C01292
         		4-(2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-1H- benzimidazol-1-yl)-2- methylbutan-2-ol m/z 379 [M + 1]
    643
    Figure US20100267698A1-20101021-C01293
         		1-ethyl-2-{[2-(3-fluoropyridin-2- yl)-1H-imidazol-1-yl]methyl}-6- methyl-1H-imidazo[4,5- c]pyridine (CDCl3) 8.96 (s, 1H), 8.46 (dt, 4.8, 1.4 Hz, 1H), 7.61 (m, 1H), 7.37 (m, 1H), 7.24 (d, J = 1.2 Hz, 1H), 7.10 (s, 1H), 7.09 (d, J = 1.2 Hz, 1H), 6.06 (s. 2H), 4.23 (q, J = 7.2 Hz, 2H), 2.66 (s, 3H), 1.10 (t, J = 7.2 Hz, 3H) m/z 337 [M + 1]
    644
    Figure US20100267698A1-20101021-C01294
         		2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-1-methyl- 6-(trifluoromethyl)-1H- benzimidazole m/z 375 [M + 1]
    645
    Figure US20100267698A1-20101021-C01295
         		2-{[2-(3-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-methyl- 6-(trifluoromethyl)-1H- benzimidazole m/z 376 [M + 1]
    646
    Figure US20100267698A1-20101021-C01296
         		2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-1,6- dimethyl-1H-imidazo[4,5- c]pyridine (CDCl3) 8.95 (s, 1H), 7.49-7.37 (m, 3H), 7.16 (m, 2H), 7.06 (s, 1H), 6.97 (s, 1H), 5.50 (s, 2H), 3.33 (s, 3H), 2.66 (s, 3H) m/z 322 [M + 1]
    647
    Figure US20100267698A1-20101021-C01297
         		1-ethyl-2-{[2-(6-fluoropyridin-2- yl)-1H-imidazol-1-yl]methyl}-6- methyl-1H-benzimidazole (CDCl3) 8.15 (dd, 1H), 7.87 (q, 1H), 7.63 (d, 1H), 7.08-7.17 (m, 4H), 6.88 (dd, 1H), 6.26 (s, 1H), 4.27 (q, 2H), 2.46 (s, 1H), 1.16 (t, 3H)
    648
    Figure US20100267698A1-20101021-C01298
         		1-(1-ethyl-2-{[2-(3- fluoropyridin-2-yl)-1H-imidazol- 1-yl]methyl}-1H-benzimidazol- 5-yl)ethanone (CDCl3) 8.45 (d, 1H), 8.38 (s, 1H), 7.98 (dd, 1H), 7.61 (t, 1H), 7.37 (m, 2H), 7.24 (s, 1H), 7.21 (s, 1H), 6.08 (s, 2H), 4.29 (q, 2H), 2.66 (s, 3H), 1.14 (t, 3H)
    649
    Figure US20100267698A1-20101021-C01299
         		1-ethyl-2-{[2-(3-fluoropyridin- 2-yl)-1H-imidazol-1-yl]methyl}- 1H-imidazo[4,5-c]pyridine (CDCl3) 9.12 (s, 1H), 8.45-8.49 (m, 2H), 7.620 (t, 1H), 7.35-7.41 (m, 1H), 7.30 (d, 1H), 7.27 (s, 1H), 7.11 (s, 1H), 6.13 (s, 2H), 4.30 (q, 2H), 1.12 (t, 3H)
    650
    Figure US20100267698A1-20101021-C01300
         		1-ethyl-2-{[2-(3-fluoropyridin- 2-yl)-1H-imidazol-1-yl]methyl}- 1H-benzimidazole-5-carbonitrile (CDCl3) 8.45 (d, 1H), 8.09 (s, 1H), 7.62 (t, 1H), 7.58 (d, 1H), 7.42 (s, 1H), 7.35-7.40 (m, 2H), 7.09 (s, 1H), 6.10 (s, 2H), 4.32 (q, 2H), 1.12 (t, 3H)
    651
    Figure US20100267698A1-20101021-C01301
         		5-chloro-1-ethyl-2-{[2-(3- fluoropyridin-2-yl)-1H-imidazol- 1-yl]methyl}-1H-benzimidazole m/z 356 [M + 1]
    652
    Figure US20100267698A1-20101021-C01302
         		1-ethyl-5-fluoro-2-{[2-(3- fluoropyridin-2-yl)-1H-imidazol- 1-yl]methyl}-1H-benzimidazole m/z 340 [M + 1]
    653
    Figure US20100267698A1-20101021-C01303
         		5-bromo-1-ethyl-2-{[2-(3- fluoropyridin-2-yl)-1H-imidazol- 1-yl]methyl}-1H-benzimidazole (CDCl3) 8.46 (s, 1H), 7.90 (s, 1H), 7.62 (t, 1H), 7.32-7.42 (m, 2H), 7.17-7.27 (m, 2H), 7.06 (s, 1H), 6.02 (s, 2H), 4.22 (q, 2H), 1.06 (t, 3H)
    654
    Figure US20100267698A1-20101021-C01304
         		9-ethyl-8-{[2-(3-fluoropyridin- 2-yl)-1H-imidazol-1-yl]methyl}- 2-methyl-9H-purine (CDCl3) 8.96 (s, 1H), 8.40 (d, 1H), 7.59 (m, 1H), 7.30 (m, 2H), 7.12 (s, 1H), 6.07 (s, 2H), 4.30 (q, 2H), 2.78 (s, 3H), 1.20 (t, 3H)
    655
    Figure US20100267698A1-20101021-C01305
         		3-ethyl-2-{[2-(6-fluoropyridin- 2-yl)-1H-imidazol-1-yl]methyl}- 3H-imidazo[4,5-c]pyridine (CDCl3) 8.84 (s, 1H), 8.47 (d, J = 5.7 Hz, 1H), 8.20 (dd, J = 7.8, 3.0 Hz, 1H), 7.91 (q, J = 8.1 Hz, 1H), 7.28 (s, 1H), 7.25 (d, J = 9.9 Hz, 2H), 6.91 (dd, J = 5.1, 3 Hz, 1H), 6.35 (s, 2H), 4.39 (q, J = 5.4 Hz, 2H), 1.49 (t, J = 5.4 Hz, 3H) m/z 323 [M + 1]
    656
    Figure US20100267698A1-20101021-C01306
         		6-ethyl-2-{[2-(6-fluoropyridin- 2-yl)-1H-imidazol-1-yl]methyl}- 1-methyl-1H-imidazo[4,5- c]pyridine (CDCl3) 8.96 (s, 1H), 8.17 (br d, J = 7.6 Hz, 1H), 7.87 (q, J = 7.8 Hz, 1H), 7.21 (s, 1H), 7.19 (s, 1H), 7.12 (s, 1H), 6.87 (m, 1H), 6.24 (s, 2H), 3.83 (s, 3H), 2.95 (q, J = 7.2 Hz, 2H), 1.36 (t, J = 7.2 Hz, 3H) m/z 337 [M + 1]
    657
    Figure US20100267698A1-20101021-C01307
         		9-ethyl-8-{[2-(6-fluoropyridin- 2-yl)-1H-imidazol-1-yl]methyl}- 2-methyl-9H-purine (CDCl3) 8.96 (s, 1H), 8.17 (br d, J = 7.6 Hz, 1H), 7.87 (q, J = 7.8 Hz, 1H), 7.21 (s, 1H), 7.19 (s, 1H), 7.12 (s, 1H), 6.87 (m, 1H), 6.24 (s, 2H), 3.83 (s, 3H), 2.95 (q, J = 7.2 Hz, 2H), 1.36 (t, J = 7.2 Hz, 3H) m/z 337 [M + 1]
    658
    Figure US20100267698A1-20101021-C01308
         		1-ethyl-2-{[2-(1,3-thiazol-2- yl)-1H-imidazol-1-yl]methyl}- 1H-benzimidazole-5-carbonitrile (DMSO) 8.05 (s, 1H), 7.80 (d, 1H), 7.73 (d, 1H), 7.65 (d, 1H), 7.60 (d, 1H), 7.48 (s, 1H), 7.10 (s, 1H), 6.20 (s, 2H), 4.40 (q, 2H), 1.35 (t, 3H)
    659
    Figure US20100267698A1-20101021-C01309
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-3-methyl- 3H-imidazo[4,5-c]pyridine (DMSO) 8.95 (s, 1H), 8.22 (d, J = 5.4 Hz, 1H), 7.99 (m, 2H), 7.54 (s, 1H), 7.43 (d, J = 5.4 Hz, 1H), 7.16 (s, 1H), 7.0 (br d, J = 8.1 Hz, 1H), 6.08 (s, 2H), 4.01 (s, 3H) m/z 309 [M + 1]
    660
    Figure US20100267698A1-20101021-C01310
         		3-ethyl-2-{[2-(3-fluoropyridin- 2-yl)-1H-imidazol-1-yl]methyl}- 3H-imidazo[4,5-c]pyridine (CDCl3) 8.81 (s, 1H), 8.46 (m, 2H), 7.64 (m, 2H), 7.36 (m, 1H), 7.25 (s, 1H), 7.12 (s, 1H), 6.11 (s, 2H), 4.38 (q, J = 7.2 Hz, 2H), 1.20 (t, J = 7.2 Hz, 3H) m/z 323 [M + 1]
    661
    Figure US20100267698A1-20101021-C01311
         		1-ethyl-2-{[2-(3-fluoropyridin- 2-yl)-1H-imidazol-1-yl]methyl}- 5-thien-3-yl-1H-benzimidazole 404.3 
    662
    Figure US20100267698A1-20101021-C01312
         		1-(1-propyl-2-{[2-(6- fluoropyridin-2-yl)-1H-imidazol- 1-yl]methyl}-1H-benzimidazol- 5-yl)ethanone (CDCl3) 8.38 (s, 1H), 8.18 (dd, 1H), 8.00 (d, 1H), 7.91 (q, 1H), 7.39 (d, 1H), 7.24 (s, 1H), 7.18 (s, 1H), 6.89 (dd, 1H), 6.31 (s, 2H), 4.28 (t, 2H), 2.67 (s, 3H), 1.69 (q, 2H), 0.88 (t, 3H)
    663
    Figure US20100267698A1-20101021-C01313
         		1-ethyl-2-{[2-(1,3-thiazol-2-yl)- 1H-imidazol-1-yl]methyl}-6- (trifluoromethyl)-1H- benzimidazole (CD3Cl3) 7.87 (m, 2H), 7.62 (s, 1H), 7.55 (d, 1H), 7.41 (d, 1H), 7.18 (s, 1h), 7.15 (s, 1H), 6.37 (s, 2H), 4.30 (q, 2H), 1.14 (t, 3H)
    664
    Figure US20100267698A1-20101021-C01314
         		1-ethyl-2-[(2-pyrimidin-2-yl-1H- imidazol-1-yl)methyl]-6- (trifluoromethyl)-1H- benzimidazole (CDCl3) 8.86 (d, 2H), 7.85 (d, 1H), 7.62 (s, 1H), 7.55 (d, 1H), 7.29 (m, 2H), 7.16 (s, 1H), 6.39 (s, 2H), 4.28 (q, 2H), 1.18 (t, 3H)
    665
    Figure US20100267698A1-20101021-C01315
         		2-({2-[6-(methylamino)pyridin-2- yl]-1H-imidazol-1-yl}methyl)-1- propyl-1H-benzimidazole-5- carbonitrile m/z 372 [M + 1]
    666
    Figure US20100267698A1-20101021-C01316
         		1-ethyl-2-{[2-(2-furyl)-1H- imidazol-1-yl]methyl}-6- (trifluoromethyl)-1H- benzimidazole m/z 361 [M + 1]
    667
    Figure US20100267698A1-20101021-C01317
         		1-ethyl-2-{[2-(1,3-thiazol-2-yl)- 1H-imidazol-1-yl]methyl}-5- (trifluoromethyl)-1H- benzimidazole (CDCl3) 8.14(s, 1H), 7.84 (d, 1H), 7.58 (d, 1H), 7.40 (m, 2H), 7.18 (s, 1H), 7.15 (s, 1H), 6.37 (s, 2H), 4.30 (q, 2H), 1.12 (t, 3H)
    668
    Figure US20100267698A1-20101021-C01318
         		1-ethyl-2-[(2-pyrimidin-2-yl-1H- imidazol-1-yl)methyl]-5- (trifluoromethyl)-1H- benzimidazole (CDCl3) 8.85 (d, 2H), 8.06 (s, 1H) < 7.54 (d, 1H), 7.40(d, 1H), 7.28 (m, 2H), 7.15 (s, 1H), 6.38 (s, 2H), 4.27 (q, 2H), 1.15 (t, 3H)
    669
    Figure US20100267698A1-20101021-C01319
         		5,6-dichloro-1-ethyl-2-{[2-(1,3- thiazol-2-yl)-1H-imidazol-1- yl]methyl}-1H-benzimidazole (CDCl3) 7.84 (m, 2H), 7.44 (s, 1H), 7.34 (d, 1H), 7.14 (m, 2H), 6.31 (s, 2H), 4.20 (q, 2H), 1.06 (t, 3H)
    670
    Figure US20100267698A1-20101021-C01320
         		5,6-dichloro-1-ethyl-2-[(2- pyrimidin-2-yl-1H-imidazol-1- yl)methyl]-1H-benzimidazole (CDCl3) 8.84 (d, 2H), 7.84 (s, 1H), 7.43 (s, 1H), 7.26 (m, 2H), 7.13 (s, 1H), 6.33 (s, 2H), 4.18 (q, 2H), 1.10 (t, 3H)
    671
    Figure US20100267698A1-20101021-C01321
         		1-ethyl-2-{[2-(1H-pyrazol-3-yl)- 1H-imidazol-1-yl]methyl}-1H- benzimidazole-5-carbonitrile (CDCl3) 7.97 (s, 1H), 7.53 (br s, 1H), 7.46 (d, J = 8.7 Hz, 1H), 7.35 (d, J = 8.1 Hz, 1H), 6.97 (d, J = 6.3 Hz, 2H), 6.79 (br s, 1H), 6.10 (s, 2H), 4.22 (q, J = 7.2 Hz, 2H), 0.88 (t, J = 7.2 Hz, 3H) m/z 318 [M + 1]
    672
    Figure US20100267698A1-20101021-C01322
         		1-ethyl-6-methyl-2-{[2-(1,3- thiazol-2-yl)-1H-imidazol-1- yl]methyl}-1H-benzimidazole (CDCl3) 7.86 (d, 1H), 7.65 (d, 1H), 7.39 (d, 1H), 7.14 (d, 1H), 7.11 (m, 3H), 6.31 (s, 2H), 4.16 (q, 2H), 1.02 (t, 3H)
    673
    Figure US20100267698A1-20101021-C01323
         		3-ethyl-2-{[2-(1,3-thiazol-2-yl)- 1H-imidazol-1-yl]methyl}-5- (trifluoromethyl)-3H-imidazo[4,5- b]pyridine (CD3OD) 8.03 (d, J = 8.4 Hz, 1H), 7.72 (d, J = 3.3 Hz, 1H), 7.65 (d, J = 8.1 Hz, 1H), 7.55 (d, J = 3.3 Hz, 1H), 7.46 (d, J = 1.5 Hz, 1H), 7.20 (d, J = 1.5 Hz, 1H), 6.3 (s, 2H), 4.56 (q, J = 6.9 Hz, 2H), 1.49 (t,, J = 6.9 Hz, 3H)
    674
    Figure US20100267698A1-20101021-C01324
         		1-ethyl-2-{[2-(1,3-thiazol-2-yl)- 1H-imidazol-1-yl]methyl}-6- (trifluoromethyl)-1H-imidazo[4,5- c]pyridine (CD3OD) 8.83 (s, 1H), 8.14 (s, 1H), 7.71 (d, J = 2.7 Hz, 1H), 7.53 (d, J = 2.7 Hz, 1H), 7.45 (d, J = 0.9 Hz, 1H), 7.19 (d, J = 0.9 Hz, 1H), 6.30 (s, 2H), 4.54 (q, J = 5.4 Hz, 2H), 1.46 (t, J = 5.4 Hz, 3H) m/z 379 [M + 1]
    675
    Figure US20100267698A1-20101021-C01325
         		3-ethyl-2-{[2-(1,3-thiazol-2-yl)- 1H-imidazol-1-yl]methyl}-6- (trifluoromethyl)-3H-imidazo[4,5- c]pyridine (CD3OD) 9.02 (s, 1H), 7.89 (s, 1H), 7.70 (d, J = 3 Hz, 1H), 7.52 (d, J = 3.3 Hz, 1H), 7.46 (d, J = 1.5 Hz, 1H), 7.19 (d, J = 1.5 Hz, 1H), 6.29 (s, 2H), 4.56 (q, J = 7.5 Hz, 2H), 1.52 (t, J = 7.5 Hz, 3H) m/z 379 [M + 1]
    676
    Figure US20100267698A1-20101021-C01326
         		1-ethyl-5-fluoro-2-{[2-(1,3- thiazol-2-yl)-1H-imidazol-1- yl]methyl}-1H-benzimidazole (CDCl3) 7.88 (d, 1H), 7.45 (dd, 1H), 7.39 (d, 1H), 7.23 (dd, 1H), 7.18 (s, 1H), 7.12 (s, 1H), 7.06 (td, 1H)
    677
    Figure US20100267698A1-20101021-C01327
         		1,6-diethyl-2-{[2-(1,3-thiazol-2- yl)-1H-imidazol-1-yl]methyl}- 1H-imidazo[4,5-c]pyridine (CDCl3) 9.01 (d, J = 1.2 Hz, 1H), 7.85 (d, J = 3.6 Hz, 1H), 7.40 (d, J = 3.0 Hz, 1H), 7.16 (d, J = 1.5 Hz, 1H), 7.14 (d, J = 0.9 Hz, 1H), 7.10 (s, 1H), 6.34 (s, 2H), 4.22 (q, J = 7.2 Hz, 2H), 2.93 (q, J = 7.2 Hz, 2H), 1.35 (t, J = 7.2 Hz, 3H), 1.10 (t, J = 7.2 Hz, 3H) m/z 339 [M + 1]
    678
    Figure US20100267698A1-20101021-C01328
         		1-(2-{[2-(6-fluoropyridin-2-yl)- 1H-imidazol-1-yl]methyl}-1- propyl-1H-imidazo[4,5- c]pyridin-4-yl)ethanone (CDCl3) 9.01 (d, J = 1.2 Hz, 1H), 7.85 (d, J = 3.6 Hz, 1H), 7.40 (d, J = 3.0 Hz, 1H), 7.16 (d, J = 1.5 Hz, 1H), 7.14 (d, J = 0.9 Hz, 1H), 7.10 (s, 1H), 6.34 (s, 2H), 4.22 (q, J = 7.2 Hz, 2H), 2.93 (q, J = 7.2 Hz, 2H), 1.35 (t, J = 7.2 Hz, 3H), 1.10 (t, J = 7.2 Hz, 3H) m/z 339 [M + 1]
    679
    Figure US20100267698A1-20101021-C01329
         		3-ethyl-2-{[2-(1,3-thiazol-2-yl)- 1H-imidazol-1-yl]methyl}-3H- imidazo[4,5-c]pyridine (CD3OD) 8.87 (s, 1H), 8.30 (d, J = 5.7 Hz, 1H), 7.75 (d, J = 3.3 Hz, 1H), 7.57 (d, J = 4.8 Hz, 1H), 7.52 (d, J = 3.3 Hz, 1H), 7.39 (d, J = 1.5 Hz, 1H), 7.18 (d, J = 1.5 Hz, 1H), 6.29 (s, 2H), 4.52 (q, J = 7.5 Hz, 2H), 1.44 (t, J = 7.5 Hz, 3H) m/z 311 [M + 1]
    680
    Figure US20100267698A1-20101021-C01330
         		1-ethyl-5-fluoro-6-methyl-2-{[2- (1,3-thiazol-2-yl)-1H-imidazol-1- yl]methyl}-1H-benzimidazole (CDCl3) 7.86 (d, 1H), 7.40 (m, 2H), 7.15 (d, 1H), 7.11 (d, 1H), 7.07 (d, 1H), 6.30 (s, 2H), 4.17 (q, 2H), 2.39 (d, 3H), 1.03 (t, 3H)
    681
    Figure US20100267698A1-20101021-C01331
         		4-(1-ethyl-2-{[2-(1,3-thiazol-2- yl)-1H-imidazol-1-yl]methyl}- 1H-benzimidazol-5-yl)-2- methylbutan-2-ol (CDCl3) 7.85 (d, 1H), 7.60 (s, 1H), 7.38 (d, 1H), 7.22 (m, 1H), 7.15 (m, 2H), 7.09 (s, 1H), 6.31 (s, 2H), 4.17 (q, 2H), 2.80 (m, 2H), 1.81 (m, 2H), 1.22 (d, 6H), 1.01 (t, 3H) m/z 396.3 [M + 1]
    682
    Figure US20100267698A1-20101021-C01332
         		1-(1-ethyl-2-{[2-(1,3-thiazol-2- yl)-1H-imidazol-1-yl]methyl}- 1H-benzimidazol-5-yl)-4- hydroxypentan-1-one (CDCl3) 8.44 (d, 1H), 7.80 (dd, 1H), 7.85 (d, 1H), 7.40 (d, 1H), 7.36 (d, 1H), 7.18 (d, 1H), 7.14 (d, 1H), 6.36 (s, 2H), 4.27 (q, 2H), 3.86 (m, 1H), 3.19 (t, 2H), 193 (m, 2H), 1.25 (d, 3H), 1.09 (t, 3H) m/z 410.2 [M + 1]
    683
    Figure US20100267698A1-20101021-C01333
         		1-ethyl-6-isobutyl-2-{[2-(1,3- thiazol-2-yl)-1H-imidazol-1- yl]methyl}-1H-imidazo[4,5- c]pyridine (CDCl3) 9.02 (s, 1H), 7.85 (d, 1H), 7.40 (d, 1h), 7.18 (s, 1H), 7.15 (s, 1H), 7.06 (s, 1H), 6.35 (s, 2H), 4.22 (q, 2H), 2.75 (d, 1H), 2.1 (m, 1H), 1.10 (t, 3H), 0.93 (d, 6H)
    684
    Figure US20100267698A1-20101021-C01334
         		1-ethyl-6-isopropyl-2-{[2-(1,3- thiazol-2-yl)-1H-imidazol-1- yl]methyl}-1H-imidazo[4,5- c]pyridine (CDCl3) 9.01 (s, 1H), 7.83 (d, J = 3.2 Hz, 1H), 7.38 (d, J = 3.2 Hz, 1H), 7.15 (s, 1H), 7.12 (s, 1H), 7.09 (s, 1H), 6.32 (s, 2H), 4.21 (q, J = 7.2 Hz, 2H), 3.17 (sept, J = 6.8 Hz, 1H), 1.34 (d, J = 6.8 Hz, 6H), 1.10 (t, J = 7.2 Hz, 3H) m/z 353 [M + 1]
    685
    Figure US20100267698A1-20101021-C01335
         		3-ethyl-6-isobutyl-2-{[2-(1,3- thiazol-2-yl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- c]pyridine (CDCl3) 8.70 (s, 1H), 7.85 (d, 1H), 7.41 (s, 1H), 7.40 (d, 1h), 7.20 (s, 1H), 7.15 (s, 1H), 6.34 (s, 2H), 4.33 (q, 2H), 2.75 (d, 1H), 2.1 (m, 1H), 1.19 (t, 3H), 0.93 (d, 6H)
    686
    Figure US20100267698A1-20101021-C01336
         		1-(1-ethyl-2-{[2-(1,3-thiazol-2- yl)-1H-imidazol-1-yl]methyl}- 1H-benzimidazol-6-yl)ethanone (CDCl3) 8.05 (d, 1H), 7.92 (dd, 1H), 7.87 (dd, 1H), 7.81 (dd, 1H), 7.40 (d, 1H), 7.20 (d, 1H), 7.14 (d, 1H), 6.17 (s, 2H), 4.33 (q, 2H), 2.67 (s, 3H), 1.13 (t, 3H)
    687
    Figure US20100267698A1-20101021-C01337
         		4-(1-ethyl-2-{[2-(1,3-thiazol-2- yl)-1H-imidazol-1-yl]methyl}- 1H-benzimidazol-6-yl)-2- methylbutan-2-ol (CDCl3) 7.88 (d, 1H), 7.68 (d, 1H), 7.39 (d, 1H), 7.12-7.18 (m, 3H), 7.09 (d, 1H), 6.13 (s, 2H), 4.19 (q, 1H), 2.80-2.89 (m, 2H), 1.80-1.89 (m, 2H), 1.31 (s, 6H), 1.06 (t, 3H)
    688
    Figure US20100267698A1-20101021-C01338
         		1-ethyl-2-{[2-(1,3-thiazol-2-yl)- 1H-imidazol-1-yl]methyl}-1H- benzimidazole-6-carbonitrile (CD3OD) (2HCl Salt) 8.38 (s, 1H), 7.98-8.01 (m, 2H), 7.95 (d, 1H), 7.87 (d, 1H), 7.71 (d, 2H), 6.50 (s, 2H), 4.62 (q, 2H), 1.62 (t, 3H)
    689
    Figure US20100267698A1-20101021-C01339
         		1-ethyl-2-[(2-pyrimidin-2-yl-1H- imidazol-1-yl)methyl]-1H- benzimidazole-5-carbonitrile (CD3OD) (2HCl Salt) 8.88 (d, 2H), 8.08 (d, 1H), 7.95 (d, 1H), 7.93 (d, 1H), 7.90 (s, 1H), 7.73 (dd, 1H), 7.56 (t, 1H), 6.60 (s, 2H), 4.61 (q, 2H), 1.63 (t, 3H)
    690
    Figure US20100267698A1-20101021-C01340
         		1-ethyl-2-[(2-pyrimidin-2-yl-1H- imidazol-1-yl)methyl]-1H- benzimidazole-6-carbonitrile (CDCl3) 8.85 (d, 2H), 7.82 (d, 1H), 7.69 (d, 1H), 7.55 (dd, 1H), 7.26-7.30 (m, 2H), 7.18 (d, 1H), 6.39 (s, 2H), 4.29 (q, 2H), 1.09 (t, 3H)
    691
    Figure US20100267698A1-20101021-C01341
         		4-chloro-1-(1-ethyl-2-{[2-(1,3- thiazol-2-yl)-1H-imidazol-1- yl]methyl}-1H-benzimidazol-5- yl)-4-methylpentan-1-one m/z 442.2 [M − 1] 444.1 [M + 1]
    692
    Figure US20100267698A1-20101021-C01342
         		1-ethyl-2-[(2-pyrimidin-2-yl-1H- imidazol-1-yl)methyl]-6- (trifluoromethyl)-1H- imidazo[4,5-c]pyridine (CD3OD) 8.79 (s, 1 H), 8.70 (d, J = 5.1 Hz, 1H), 8.17 (s, 1H), 7.52 (d, J = 1.2 Hz, 1H), 7.31 (d, J = 1.2 Hz, 1H), 7.27 (t, J = 5.1 Hz, 1H), 6.63 (s, 1H), 6.32 (s, 2H), 4.57 (q, J = 7.5 Hz, 2H), 1.50 (t,, J = 7.5 Hz, 3H) m/z 374 [M + 1]
    693
    Figure US20100267698A1-20101021-C01343
         		2-{1-[(5-acetyl-1-ethyl-1H- benzimidazol-2-yl)methyl]-1H- imidazol-2-yl}-1,3-thiazole-4- carbonitrile (CDCl3 + CD3OD) 8.29 (s, 1H), 8.25 (d, 1H), 7.97 (dd, 1H), 7.52 (d, 1H), 7.42 (s, 1H), 7.22 (s, 1H), 6.17 (s, 2H), 4.44 (q, 2H), 2.64 (s, 3H), 1.43 (t, 3H) m/z 377 [M + 1]
    694
    Figure US20100267698A1-20101021-C01344
         		5-bromo-1-ethyl-2-[(2- pyrimidin-2-yl-1H-imidazol-1- yl)methyl]-1H-benzimidazole (CDCl3) 8.85 (d, 2H), 7.91 (d, 1H), 7.39 (dd, 1H), 7.27 (t, 1H), 7.25 (m, 1H), 7.19 (d, 1H), 7.13 (d, 1H), 6.35 (s, 2H), 4.20 (q, 2H), 1.08 (t, 3H)
    695
    Figure US20100267698A1-20101021-C01345
         		1-(3-ethyl-2-{[2-(1,3-thiazol-2- yl)-1H-imidazol-1-yl]methyl}- 3H-imidazo[4,5-c]pyridin-6- yl)ethanone (CDCl3) 8.79 (d, 2H), 8.47 (d, 1H), 7.83 (d, 1H), 7.40 (d, 1H), 7.19 (d, 1H), 7.15 (d, 1H), 6.39 (s, 2H), 4.02 (q, 2H), 2.77 (s, 3H), 1.86 (t, 3H) m/z 353.2 [M + 1]
    696
    Figure US20100267698A1-20101021-C01346
         		3-ethyl-6-methyl-2-{[2-(1,3- thiazol-2-yl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- c]pyridine (CDCl3) 8.67 (s, 1H), 7.83 (d, J = 3.0 Hz, 1H), 7.5 (s, 1H), 7.38 (d, J = 3.0 Hz, 1H), 7.18 (d, J = 1.2 Hz, 1H), 7.14 (d, J = 1.2 Hz, 1H), 6.33 (s, 2H), 4.32 (q, J = 7.5 Hz, 2H), 1.16 (t, J = 7.5 Hz, 3H) m/z 325 [M + 1]
    697
    Figure US20100267698A1-20101021-C01347
         		1-ethyl-5-pyridin-2-yl-2-[(2- pyrimidin-2-yl-1H-imidazol-1- yl)methyl]-1H-benzimidazole m/z 382.2 [M + 1]
    698
    Figure US20100267698A1-20101021-C01348
         		1-ethyl-6-methyl-2-{[2-(1,3- thiazol-2-yl)-1H-imidazol-1- yl]methyl}-1H-imidazo[4,5- c]pyridine (CDCl3) 8.96 (d, J = 0.9 Hz, 1H), 7.84 (d, J = 3.3 Hz, 1H), 7.39 (d, J = 3.0 Hz, 1H), 7.16 (d, J = 0.9 Hz, 1H), 7.13 (d, J = 1.5 Hz, 1H), 7.10 (s, 1H), 6.33 (s, 2H), 4.21 (q, J = 7.2 Hz, 2H), 2.66, (s, 2H), 1.09 (t, J = 7.2 Hz, 3H) m/z 325 [M + 1]
    699
    Figure US20100267698A1-20101021-C01349
         		6-(cyclopentylmethyl)-1-ethyl-2- {[2-(1,3-thiazol-2-yl)-1H- imidazol-1-yl]methyl}-1H- imidazo[4,5-c]pyridine (CDCl3) 9.01 (s, 1H), 7.85 (d, 1H), 7.41 (d, 1H), 7.18 (d, 1H), 7.15 (d, 1H), 7.08 (s, 1H), 6.36 (s, 2H), 4.22 (q, 2H), 2.86 (d, 2H), 2.25-2.40 (m, 1H), 1.46-1.78 (m, 6H), 1.15-1.30 (m, 2H), 1.08 (t, 3H)
    700
    Figure US20100267698A1-20101021-C01350
         		6-(cyclopentylmethyl)-3-ethyl-2- {[2-(1,3-thiazol-2-yl)-1H- imidazol-1-yl]methyl}-3H- imidazo[4,5-c]pyridine (CDCl3) 8.68 (s, 1H), 7.84 (d, 1H), 7.48 (s, 1H), 7.41 (d, 1H), 7.20 (s, 1H), 7.15 (s, 1H), 6.14 (s, 2H), 4.32 (q, 2H), 2.88 (d, 2H), 2.25-2.38 (m, 1H), 1.45-72 (m, 6H), 1.16-1.33 (m, 5H)
    701
    Figure US20100267698A1-20101021-C01351
         		3-ethyl-6-isopropyl-2-{[2-(1,3- thiazol-2-yl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- c]pyridine (CDCl3) 8.70 (d, J = 1.2 Hz, 1H), 7.83 (d, J = 3.0 Hz, 1H), 7.53 (d, J = 0.9 Hz, 1H), 7.39 (d, J = 3.0 Hz, 1H), 7.18 (d, J = 0.9 Hz, 1H), 7.14 (d, J = 0.9 Hz, 1H), 6.32 (s, 2H), 4.32 (q, J = 7.2 Hz, 2H), 3.18 (sept, J = 6.9 Hz, 1H), 1.34 (d, J = 6.9 Hz, 6H), 1.17 (t, J =
    Figure US20100267698A1-20101021-P00899
    		 m/z 353 [M + 1]
    702
    Figure US20100267698A1-20101021-C01352
         		1-ethyl-2-[(2-pyrimidin-2-yl-1H- imidazol-1-yl)methyl]-1H- benzimidazole m/z 305 [M + 1]
    703
    Figure US20100267698A1-20101021-C01353
         		6-chloro-1-ethyl-2-[(2-pyrimidin- 2-yl-1H-imidazol-1-yl)methyl]- 1H-benzimidazole m/z 339 [M + 1]
    704
    Figure US20100267698A1-20101021-C01354
         		1-ethyl-6-methyl-2-[(2-pyrimidin- 2-yl-1H-imidazol-1-yl)methyl]- 1H-benzimidazole m/z 319 [M + 1]
    705
    Figure US20100267698A1-20101021-C01355
         		1-ethyl-5-fluoro-2-[(2-pyrimidin- 2-yl-1H-imidazol-1-yl)methyl]- 1H-benzimidazole m/z 323 [M + 1]
    706
    Figure US20100267698A1-20101021-C01356
         		5-chloro-1-ethyl-2-[(2- pyrimidin-2-yl-1H-imidazol-1- yl)methyl]-1H-benzimidazole m/z 339 [M + 1]
    707
    Figure US20100267698A1-20101021-C01357
         		3-ethyl-6-propyl-2-{[2-(1,3- thiazol-2-yl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- c]pyridine (CDCl3) 8.69 (d, J = 0.9 Hz, 1H), 7.83 (d, J = 3.3 Hz, 1H), 7.49 (d, J = 1.2 Hz, 1H), 7.39 (d, J = 3.6 Hz, 1H), 7.18 (d, J = 0.9 Hz, 1H), 7.14 (d, J = 1.5 Hz, 1H), 6.33 (s, 2H), 4.31 (q, J = 7.2 Hz, 2H), 2.86 (m, 2H), 1.78 (sext, J = 7.2 Hz, 2H), 1.17 (t, J = 7.2 Hz, 3H), 0.97 (t, J = 7.2 Hz, 3H m/z 353 [M + ]
    708
    Figure US20100267698A1-20101021-C01358
         		1-ethyl-6-propyl-2-{[2-(1,3- thiazol-2-yl)-1H-imidazol-1- yl]methyl}-1H-imidazo[4,5- c]pyridine (CDCl3) 9.00 (d, J = 0.9 Hz, 1H), 7.84 (d, J = 3.0 Hz, 1H), 7.39 (d, J = 3.3 Hz, 1H), 7.16 (d, J = 1.5 Hz, 1H), 7.13 (d, J = 1.5 Hz, 1H), 7.09 (s, 1H), 6.33 (s, 2H), 4.22 (q, J = 7.2 Hz, 2H), 2.86 (m, 2H), 1.79 (sext, J = 7.2 Hz, 2H), 1.09 (t, J = 7.2 Hz, 3H), 0.96 (t, J = 7.2 Hz, 3H) m/z 353 [M + 1]
    709
    Figure US20100267698A1-20101021-C01359
         		3,6-diethyl-2-{[2-(1,3-thiazol-2- yl)-1H-imidazol-1-yl]methyl}- 3H-imidazo[4,5-c]pyridine (CDCl3) 8.69 (d, J = 0.9 Hz, 1H), 7.84 (d, J = 3.6 Hz, 1H), 7.51 (s, 1H), 7.39 (d, J = 3.6 Hz, 1H), 7.18 (d, J = 1.5 Hz, 1H), 7.14 (d, J = 0.9 Hz, 1H), 6.33 (s, 2H), 4.32 (q, J = 7.2 Hz, 2H), 2.93 (q, J = 7.2 Hz, 2H), 1.34 (t, J = 7.2 Hz, 3H), 1.17 (t, J = 7.2 Hz, 3H) m/z 339 [M + 1]
    710
    Figure US20100267698A1-20101021-C01360
         		1-ethyl-5-fluoro-6-methyl-2-[(2- pyrimidin-2-yl-1H-imidazol-1- yl)methyl]-1H-benzimidazole m/z 337.4 [M + 1]
    711
    Figure US20100267698A1-20101021-C01361
         		1-ethyl-2-{[2-(2-fluoro-6- methoxyphenyl)-1H-imidazol-1- yl]methyl}-1H-imidazo[4,5- c]pyridine (CDCl3) 9.04 (d, J = 0.9 Hz, 1H), 8.42 (d, J = 5.4 Hz, 1H), 7.46- 7.38 (m, 1H), 7.25-7.22 (m, 2H), 7.00 (d, J = 0.9 Hz, 1H), 6.85- 6.76 (m, 2H), 5.31, 5.22 (AB, J = 15.3 Hz, 2H), 3.85 (q, J = 7.2 Hz, 2H), 3.77 (s, 3H), 1.00 (t, J = 7.2 Hz, 3H) m/z 352 [M + 1]
    712
    Figure US20100267698A1-20101021-C01362
         		1-ethyl-2-[(2-pyrimidin-2-yl-1H- imidazol-1-yl)methyl]-1H- imidazo[4,5-c]pyridine (CDCl3) 9.09 (s, 1H), 8.85 (d, 2H), 8.46 (d, 1H), 7.26-7.30 (m, 3H), 7.16 (1H), 6.39 (s, 2H), 4.25 (q, 2H), 1.15 (t, 3H)
    713
    Figure US20100267698A1-20101021-C01363
         		1-ethyl-6-methyl-2-[(2-pyrimidin- 2-yl-1H-imidazol-1-yl)methyl]- 1H-imidazo[4,5-c]pyridine (CDCl3) 8.95 (d, J = 1.2 Hz, 1H), 8.84 (d, J = 4.8 Hz, 2H), 7.27 (t, J = 5.1 Hz, 1H), 7.25 (d, J = 1.2 Hz, 1H), 7.13 (d, J = 1.2 Hz, 1H), 7.10 (s, 1H), 6.35 (s, 2H), 4.19 (q, J = 7.2 Hz, 2H), 2.65 (s, 3H), 1.12 (t, J = 7.2 Hz, 3H) m/z 320 [M + 1]
    714
    Figure US20100267698A1-20101021-C01364
         		3-ethyl-2-[(2-pyrimidin-2-yl-1H- imidazol-1-yl)methyl]-3H- imidazo[4,5-c]pyridine (DMSO) 8.94 (s, 1 H), 8.71 (d, J = 5.1 Hz, 2H), 8.22 (d, J = 5.7 Hz, 1H), 7.53 (d, J = 1.2 Hz, 1H), 7.43 (d, J = 5.4 Hz, 1H), 7.31 (t, J = 4.5 Hz, 1H), 7.17 (d, J = 1.2 Hz, 1H), 6.22 (s, 2H), 4.46 (q, J = 7.5 Hz, 2H), 1.39 (t,, J = 7.5 Hz, 3H) m/z 306 [M + 1]
    715
    Figure US20100267698A1-20101021-C01365
         		3-ethyl-2-[(2-pyrimidin-2-yl-1H- imidazol-1-yl)methyl]-3H- imidazo[4,5-c]pyridine-4- carbonitrile (CD3OD) 8.70 (d, J = 5.1 Hz, 2 H), 8.41 (d, J = 5.4 Hz, 1H), 7.73 (d, J = 5.1 Hz, 1H), 7.52 (d, J = 1.2 Hz, 1H), 7.31 (d, J = 1.2 Hz, 1H), 7.27 (t, J = 4.5 Hz, 1H), 6.35 (s, 2H), 4.75 (q, J = 7.5 Hz, 2H), 1.63 (t,, J = 7.2 Hz, 3H) m/z 331 [M + 1]
    716
    Figure US20100267698A1-20101021-C01366
         		1-ethyl-6-isopropyl-2-[(2- pyrimidin-2-yl-1H-imidazol-1- yl)methyl]-1H-imidazo[4,5- c]pyridine (CDCl3) 9.01 (d, J = 0.9 Hz, 1H), 8.84 (d, J = 4.8 Hz, 2H), 7.28 (t, J = 4.8 Hz, 1H), 7.25 (d, J = 1.2 Hz, 1H), 7.13 (d, J = 0.9 Hz, 1H), 7.10 (d, J = 0.9 Hz, 1H), 6.35 (s, 2H), 4.21 (q, J = 7.2 Hz, 2H), 3.17 (sept, J = 6.9 Hz, 1H), 1.34 (d, J = 6.9 Hz, 6H), 1.13 (t, J = 7.2 Hz, 3H) m/z 348 [M + 1]
    717
    Figure US20100267698A1-20101021-C01367
         		{1-ethyl-2-[(2-pyrimidin-2-yl- 1H-imidazol-1-yl)methyl]-1H- benzimidazol-5- yl}(phenyl)methanone (CDCl3) 8.84 (d, 2H), 8.20 (s, 1H), 7.80-7.90 (m, 3H), 7.40-7.60 (m, 4H), 7.3 (m, 3H), 7.18 (s, 1H), 6.38 (s, 2H), 4.25 (q, 2H), 1.22 (t, 3H)
    718
    Figure US20100267698A1-20101021-C01368
         		1-{1-ethyl-2-[(2-pyrimidin-2-yl- 1H-imidazol-1-yl)methyl]-1H- benzimidazol-6-yl}ethanone (CDCl3) 8.84 (d, 2H), 8.03 (s, 1H), 7.90 (d, 1H), 7.78 (d, 1H), 7.25-7.28 (m, 2H), 7.18 (s, 1H), 6.38 (s, 2H), 4.48 (q, 2H), 2.66 (s, 3H), 1.08 (t, 3H)
    719
    Figure US20100267698A1-20101021-C01369
         		1-ethyl-6-fluoro-2-[(2-pyrimidin- 2-yl-1H-imidazol-1-yl)methyl]- 1H-benzimidazole (CDCl3) 8.87 (d, J = 5.1 Hz, 1 H), 7.71 (q, J = 4.5 Hz, 1H), 7.27 (m, 3H), 7.14 (s, 1H), 7.03 (m, 2H), 6.34 (s, 2H), 4.18 (q, J = 7.5 Hz, 2H), 1.11 (t, J = 7.5 Hz, 3H) m/z 323 [M + 1]
    720
    Figure US20100267698A1-20101021-C01370
         		1-ethyl-6-fluoro-2-{[2-(1,3- thiazol-2-yl)-1H-imidazol-1- yl]methyl}-1H-benzimidazole (CDCl3) 7.86 (d, J = 3.3 Hz, 1 H), 7.70 (q, J = 4.8 Hz, 1H), 7.40 (d, J = 3 Hz, 1H), 7.25 (s, 1H), 7.16 (d, J = 1.2 Hz, 1H), 7.01 (m, 2H), 6.32 (s, 2H), 4.19 (q, J = 6.9 Hz, 2H), 1.06 (t,, J = 7.5 Hz, 3H) m/z 328 [M + 1]
    721
    Figure US20100267698A1-20101021-C01371
         		1-ethyl-5-phenyl-2-[(2- pyrimidin-2-yl-1H-imidazol-1- yl)methyl]-1H-benzimidazole m/z 381.5 [M + 1]
    722
    Figure US20100267698A1-20101021-C01372
         		6-(cyclopentylmethyl)-1-ethyl-2- [(2-pyrimidin-2-yl-1H-imidazol- 1-yl)methyl]-1H-imidazo[4,5- c]pyridine (CDCl3) 9.00 (s, 1H), 8.86 (d, 2H), 7.25-7.31 (m, 2H), 7.15 (s, 1H), 7.08 (s, 1H), 6.37 (s, 2H), 4.20 (q, 2H), 2.87 (d, 2H), 2.25- 2.40 (m, 1H), 1.42-1.78 (m, 6H), 1.15-1.30 (m, 2H), 1.13 (t, 3H)
    723
    Figure US20100267698A1-20101021-C01373
         		6-(cyclopentylemthyl)-3-ethyl-2- [(2-pyrimidin-2-yl-1H-imidazol- 1-yl)methyl]-3H-imidazo[4,5- c]pyridine (CDCl3) 8.85 (d, 2H), 8.69 (s, 1H), 7.47 (s, 1H), 7.25-7.29 (m, 2H), 7.18 (s, 1H), 6.38 (s, 2H), 4.30 (q, 2H), 2.88 (d, 2H), 2.22- 2.39 (m, 1H), 1.42-1.78 (m, 6H), 1.20-1.33 (m, 5H)
    724
    Figure US20100267698A1-20101021-C01374
         		1-ethyl-5-isopropyl-2-[(2- pyrimidin-2-yl-1H-imidazol-1- yl)methyl]-1H-benzimidazole (CDCl3) 8.85 (d, 2H), 7.63 (d, 1H), 7.24 (m, 2H), 7.21 (m, 2H), 7.13 (d, 1H), 6.33 (s, 2H), 4.14 (q, 2H), 3.02 (m, 1H), 1.29 (d, 6H), 1.08 (t, 3H)
    725
    Figure US20100267698A1-20101021-C01375
         		1-ethyl-6-(2-fluorophenyl)-2-[(2- pyrimidin-2-yl-1H-imidazol-1- yl)methyl]-1H-benzimidazole (CDCl3) 8.87 (d, J = 4.8 Hz, 2H), 7.85 (d, J = 8.4 Hz, 1H), 7.48 (m, 3H), 7.22 (m, 6H), 6.39 (s, 2H), 4.25 (q, J = 7.2 Hz, 2H), 1.16 (t, J = 7.2 Hz, 3H) m/z 399 [M + 1]
    726
    Figure US20100267698A1-20101021-C01376
         		1-ethyl-2-{[2-(1,3-thiazol-2-yl)- 1H-imidazol-1-yl]methyl}-6-[4- (trifluoromethyl)phenyl]-1H- benzimidazole (DMSO) 7.95 (m, 3H), 7.79 (m, 3H), 7.70 (d, J = 3.3 Hz, 1H), 7.59 (d, J = 8.7 Hz, 1H), 7.52 (s, 1H), 7.48 (s, 1H), 6.19 (s, 2H), 4.43 (q, J = 7.2 Hz, 2H), 1.34 (t, J = 7.2 Hz, 3H) m/z 454 [M + 1]
    727
    Figure US20100267698A1-20101021-C01377
         		1-ethyl-2-[(2-pyrimidin-2-yl-1H- imidazol-1-yl)methyl]-6-[4- (trifluoromethyl)phenyl]-1H- benzimidazole (CDCl3) 8.87 (d, J = 4.8 Hz, 2H), 8.00 (s, 1H), 7.86 (d, J = 8.4 Hz, 1H), 7.71 (m, 3H), 7.52 (m, 2H), 7.27 (t, J = 4.8 Hz, 1H), 7.25 (s, 1H), 7.17 (s, 1H), 6.39 (s, 2H), 4.28 (q, J = 6.9 Hz, 2H), 1.16 (t, J = 6.9 Hz, 3H) m/z 449 [M + 1]
    728
    Figure US20100267698A1-20101021-C01378
         		6-isopropyl-3-propyl-2-{[2-(1,3- thiazol-2-yl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- c]pyridine (CDCl3) 8.69 (d, J = 1.2 Hz, 1H), 7.83 (d, J = 3.3 Hz, 1H), 7.52 (s, 1H), 7.39 (d, 3.0 Hz, 1H), 7.21 (d, J = 1.5 Hz, 1H), 7.13 (d, J = 0.9 Hz, 1H), 6.33 (s, 2H), 4.21 (m, 2H), 3.18 (sept, J = 6.9 Hz, 1H), 1.61 (sext, J = 7.2 Hz, 2H), 1.34 (d, J = 6.9 Hz, 6H), 0.77 (t, J = 7.2 Hz, 3H) m/z 367 [M + 1]
    729
    Figure US20100267698A1-20101021-C01379
         		6-isopropyl-1-porpyl-2-{[2-(1,3- thiazol-2-yl)-1H-imidazol-1- yl]methyl}-1H-imidazo[4,5- c]pyridine (CDCl3) 9.01 (d, J = 0.9 Hz, 1H), 7.85 (d, J = 3.6 Hz, 1H), 7.39 (d, J = 3.6 Hz, 1H), 7.18 (d, J = 0.9 Hz, 1H), 7.12 (d, J = 0.9 Hz, 1H), 7.07 (s, 1H), 6.33 (s, 2H), 4.10 (m, 2H), 3.16 (sept, J = 6.9 Hz, 1H), 1.52 (sext, J = 7.2 Hz, 2H), 1.34 (d, J = 6.9 Hz, 6H), 0.75 (t, J = 7.2 Hz, 3H) m/z 367 [M + 1]
    730
    Figure US20100267698A1-20101021-C01380
         		3-ethyl-6-phenyl-2-{[2-(1,3- thiazol-2-yl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- c]pyridine (CDCl3) 8.85 (d, 1H), 8.08 (d, 1H), 8.02 (d, 1H), 7.99 (m, 1H), 7.84 (d, 1H), 7.48 (m, 2H), 7.40 (m, 2H), 7.22 (d, 1H), 7.17 (d, 1H), 6.36 (s, 2H), 4.38 (q, 2H), 1.22 (t, 3H) m/z 387.4 [M + 1]
    731
    Figure US20100267698A1-20101021-C01381
         		3-(2-fluoroethyl)-2-[(2- pyrimidin-2-yl-1H-imidazol-1- yl)methyl]-3H-imidazo[4,5- c]pyridine (CD3OD) 8.91 (s, 1H), 8.69 (d, J = 5.1 Hz, 2H), 8.28 (d, J = 5.7 Hz, 1H), 7.52 (m, 2H), 7.30 (s, 1H), 7.26 (t, J = 4.8 Hz, 1H), 6.25 (s, 2H) m/z 324 [M + 1]
    732
    Figure US20100267698A1-20101021-C01382
         		3-(1-ethyl-2-{[2-(1,3-thiazol-2- yl)-1H-imidazol-1-yl]methyl}- 1H-benzimidazol-6- yl)benzonitrile (DMSO) 8.24 (s, 1H), 8.09 (d, J = 7.8 Hz, 1H), 8.0 (s, 1H), 7.76 (m, 2H), 7.61 (m, 5H), 7.11 (s, 1H), 6.18 (s, 2H), 4.42 (q, J = 7.5 Hz, 2H), 1.34 (t, J = 7.5 Hz, 3H) m/z 411 [M + 1]
    733
    Figure US20100267698A1-20101021-C01383
         		3-{1-ethyl-2-[(2-pyrimidin-2-yl- 1H-imidazol-1-yl)methyl]-1H- benzimidazol-6-yl}benzonitrile (DMSO)
    Figure US20100267698A1-20101021-P00899
    8.24 (s, 1H), 8.07 (d, J = 8.4 Hz, 1H), 7.99 (s, 1H), 7.77 (d, J = 7.8 Hz, 1H), 7.64 (t,, J = 8.1 Hz, 1H), 7.50 (m, 3H), 7.34 (t, J = 4.8 Hz, 1H), 7.17 (s, 1H), 6.17 (s, 2H), 4.42 (q, J = 6.9 Hz, 2H), 1.34 (t,
    Figure US20100267698A1-20101021-P00899
    		 m/z 406 [M + 1]
    734
    Figure US20100267698A1-20101021-C01384
         		1-ethyl-2-{[2-(1,3-thiazol-2-yl)- 1H-imidazol-1-yl]methyl}-5- (trifluoromethoxy)-1H- benzimidazole (CDCl3) 7.86 (d, 1H), 7.66 (s, 1H), 7.40 (d, 1H), 7.33 (d, 1H), 7.26 (d, 2H), 7.14 (s, 1H), 6.34 (s, 2H), 4.26 (q, 2H), 1.10 (t, 3H) m/z 394 [M + 1]
    735
    Figure US20100267698A1-20101021-C01385
         		1-ethyl-2-[(2-pyrimidin-2-yl-1H- imidazol-1-yl)methyl]-5- (trifluoromethoxy)-1H- benzimidazole (CDCl3) 8.84 (d, 2H), 7.63 (s, 1H), 7.25-7.28 (m, 3H), 7.15 (d, 2H), 6.35 (s, 2H), 4.22 (q, 2H), 1.13 (t, 3H)
    736
    Figure US20100267698A1-20101021-C01386
         		3-ethyl-2-{[2-(6-fluoropyridin- 2-yl)-1H-imidazol-1-yl]methyl}- 6-isopropyl-3H-imidazo[4,5- c]pyridine (CDCl3) 8.71 (d, J = 1.2 Hz, 1H), 8.17 (dd, J = 7.7, 1.7 Hz, 1H), 7.88 (q, J = 7.9 Hz, 1H), 7.51 (d, J = 0.9 Hz, 1H), 7.21 (d, J = 0.9 Hz, 1H), 7.18 (d, J = 1.5 Hz, 1H), 6.88 (dd, J = 8.3, 2.0 Hz, 1H), 6.26 (s, 2H), 4.42 (q, J = 7.2 Hz, 2H), 3.17 (sept, J = 6.9 Hz, 1H), 1.33 (d, J = 6.9 Hz, 6H), 1.30 (t, J = 7.2 Hz, 3H) m/z 365 [M + 1]
    737
    Figure US20100267698A1-20101021-C01387
         		1-ethyl-2-{[2-(6-fluoropyridin-2- yl)-1H-imidazol-1-yl]methyl}-6- isopropyl-1H-imidazo[4,5- c]pyridine (CDCl3) 8.99 (d, J = 0.9 Hz, 1H), 8.16 (dd, J = 7.8, 1.5 Hz, 1H), 7.88 (q, J = 7.8 Hz, 1H), 7.18 (d, J = 0.9 Hz, 1H), 7.16 (d, J = 1.5 Hz, 1H), 7.11 (d, 0.9 Hz, 1H), 6.89 (dd, J = 7.8, 2.4 Hz, 1H), 6.27 (s, 2H), 4.33 (q, J = 7.2 Hz, 2H), 3.17 (sept, J = 6.9 Hz, 1H), 1.35 (d, J = 6.9 Hz, 6H), 1.23 (t, J = 7.2 Hz, 3H) m/z 365 [M + 1]
    738
    Figure US20100267698A1-20101021-C01388
         		3-ethyl-2-{[2-(6-fluoropyridin-2- yl)-1H-imidazol-1-yl]methyl}-6- propyl-3H-imidazo[4,5- c]pyridine (CDCl3)
    Figure US20100267698A1-20101021-P00899
    8.17 (dd, J = 8.0, 2.6 Hz, 1H), 7.89 (q, J = 7.8 Hz, 1H), 7.47 (s, 1H), 7.22 (d, J = 0.9 Hz, 1H), 7.18 (d, J = 1.5 Hz, 1H), 6.88 (dd, J = 8.6, 2.9 Hz, 1H), 6.27 (s, 2H), 4.42 (q, J = 7.2 Hz, 2H), 2.86 (m, 2H), 1.78 (sextet, J = z,899     		m/z 365 [M + 1]
    739
    Figure US20100267698A1-20101021-C01389
         		(CD3OD) 8.60 (d, J = 4.5 Hz, 1H), 8.09 (s, 1H), 7.88 (m, 4H), 768 (d, J = 8.4 Hz, 1H), 7.57 (d, J = 3.3 Hz, 1H), 7.34 (m, 2H), 715 (s, 1H), 6.31 (s, 2H), 4.45 (q, J = 7.5 Hz, 2H), 1.32 (t, J = 7.5 Hz, 3H) m/z 387 [M + 1]
    740
    Figure US20100267698A1-20101021-C01390
         		(CD3OD) 8.77 (m, 2H), 8.14 (s, 1H), 7.91 (m, 2H), 7.81 (d, J = 8.4 Hz, 1H), 7.62 (d, J = 8.4 Hz, 1H), 7.45 (s, 1H), 7.32 (m, 4H), 6.30 (s, 2H), 4.50 (q, J = 7.2 Hz, 2H), 1.42 (t, J = 7.2 Hz, 3H) m/z 382 [M + 1]
    741
    Figure US20100267698A1-20101021-C01391
         		(CD3OD) 8.11 (s, 1H), 7.78-7.91 (m, 3H), 7.55-7.62 (m, 4H), 7.42 (d, J = 1.5 Hz, 1H), 6.25 (s, 2H), 4.46 (q, J = 7.5 Hz, 2H), 1.37 (t, J = 7.5 Hz, 3H) m/z 393 [M + 1]
    742
    Figure US20100267698A1-20101021-C01392
         		1-ethyl-2-{[2-(3-fluorophenyl)- 1H-imidazol-1-yl]methyl}-1H- benzimidazole 321.3  [M + 1]
    743
    Figure US20100267698A1-20101021-C01393
         		2-{[2-(6-fluoropyridin-2-yl)- 1H-imidazol-1-yl]methyl}-1- propyl-1H-imidazo[4,5- b]pyridine (HCl salt) H-1 NMR (dmso): 1.05 (t, 3H), 2.03 (h, 2H), 4.62 (t, 2H), 6.58 (s, 2H), 7.24 (d, 1H), 7.82 (t, 1H), 7.95 (s, 1H), 8.05 (m, 2H), 8.25(q, 1H), 8.62(d, 1H), 8.93 (d, 1H 337   [M + 1]
    744
    Figure US20100267698A1-20101021-C01394
         		3-ethyl-5-fluoro-2-{[2-(3- fluorophenyl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine dihydrochloride 1H NMR (d6 DMSO): 8.18(1H, t), 7.99(1H, d), 7.94(1H, d), 7.72-7.69(1H, m), 7.66- 7.61(1H, m), 7.58-7.50(2H, m), 7.03(1H, d), 5.96(2H, s), 4.23(2H, q), 1.31(3H, s) 340.2  [M + 1]
    745
    Figure US20100267698A1-20101021-C01395
         		3-ethyl-2-{[2-(6-fluoropyridin-2- yl)-1H-imidazol-1-yl]methyl}- 3H-imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.39(dd, 1H), 8.19(m, 1H), 8.02(dd, 1H), 7.89(m, 1H), 7.20-7.26(m, 3H), 6.89(m, 1H), 6.32(s, 2H), 4.47(q, 2H), 1.30(t, 3H) 323.3  [M + 1]
    746
    Figure US20100267698A1-20101021-C01396
         		1-ethyl-2-{[2-(6-fluoropyridin-2- yl)-1H-imidazol-1-yl]methyl}- 1H-imidazo[4,5-b]pyridine (HCl salt) H-1 NMR (dmso): 1.63(t, 3H), 4.72(q, 2H), 6.58(s, 2H), 7.30 (d, 1H), 7.82(m, 1H), 7.96(s, 1H), 8.03(m, 2H), 8.24(q, 1H), 8.60(d, 1H), 8.90(d, 1H) 323   [M + 1]
    747
    Figure US20100267698A1-20101021-C01397
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-N,N- dimethyl-1-propyl-1H- benzimidazole-5-carboxamide CDCl3. 8.17 (d, J = 7.42 Hz, 1H), 7.85-7.92 (m, 1H), 7.80 (s, 1H), 7.34-7.42 (m, 2H). 7.21 (s, 1H), 7.15 (s, 1H), 6.89 (d, J = 7.97 Hz, 1H), 6.29 (s, 2H), 4.21-4.26 (m, 2H), 3.04-3.11 (br d, 6H), 1.61-1.69 (m, 2H), 0.80(t, J = 7.42 Hz, 3H).
    748
    Figure US20100267698A1-20101021-C01398
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-5-(5- methyl-1,2,4-oxadiazol-3-yl)-1- propyl-1H-benzimidazole d6-DMSO: 7.94-8.09 (m, 3H), 7.82 (d, 1H), 7.76 (d, 1H), 7.47 (s, 1H), 7.18 (s, 1H), 7.03 (d, 1H), 6.15 (s, 2H), 4.35 (t, 2H), 2.65 (s, 3H), 1.74- 1.82 (m, 2H), 0.94 (t, 3H). 418.3  [M + 1]; 416.2  [M − 1]
    749
    Figure US20100267698A1-20101021-C01399
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-5-(5- methyl-1,3,4-oxadiazol-2-yl)-1- propyl-1H-benzimidazole CDCl3: 8.36 (s, 1H), 8.18 (dd, 1H), 8.03 (dd, 1H), 7.89 (q, 1H), 7.44 (d, 1H), 7.25 (s, 1H), 7.17 (s, 1H), 6.90 (dd, 1H), 6.33 (s, 2H), 4.29 (t, 2H), 2.59 (s, 3H), 1.59-1.74 (m, 2H), 0.82 (t, 3H). 418.3  [M + 1]; 416.2  [M − 1]
    750
    Figure US20100267698A1-20101021-C01400
         		2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-3-ethyl-5- fluoro-3H-imidazo[4,5-b]pyridine hydrochloride 1H NMR (d6 DMSO): 8.15(1H, t), 8.08(1H, d), 7.99(1H, d), 7.76-7.72(1H, m), 7.62- 7.50(2H, m), 7.02(1H, d), 5.92(2H, s), 4.20(2H, q), 1.26(3H, t) 358.2  [M + 1]
    751
    Figure US20100267698A1-20101021-C01401
         		3-{1-[(3-ethyl-5-fluoro-3H- imidazo[4,5-b]pyridin-2- yl)methyl]-1H-imidazol-2- yl}benzonitrile hydrochloride 1H NMR (d6 DMSO): 8.30(1H, s), 8.16(1H, dd), 8.11(1H, d), 8.04(1H, d), 8.00(1H, d), 7.96(1H, d), 7.74(1H, t), 7.03(1H, d), 5.97(2H, s), 4.22(2H, q), 1.30(3H, t) 347.3  [M + 1]
    752
    Figure US20100267698A1-20101021-C01402
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-propyl- 1H-imidazo[4,5-c]pyridine 1H NMR (CDCl3) δ 9.06 (s, 1 H), 8.42 (d, 1 H), 8.18 (d, 1 H), 7.88 (dd, 1 H), 7.28 (d, 1 H), 7.22 (d, 1 H), 7.18 (d, 1 H), 6.88 (d, 1 H), 6.29 (s, 2 H), 4.26 (t, 2 H), 1.69 (q, 2 H), 0.84 (t, 3 H) m/e 337
    753
    Figure US20100267698A1-20101021-C01403
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1- isopropyl-1H-imidazo[4,5- c]pyridine 1H NMR (CDCl3) δ 9.03 (s, 1 H), 8.35 (d, 1 H), 8.14 (d, 1 H), 7.87 (dd, 1 H), 7.42 (d, 1 H), 7.15 (d, 1 H), 7.09 (d, 1 H), 6.87 (d, 1 H), 6.28 (s, 2 H), 5.08 (septet, 1 H), 1.49 (d, 6 H) m/e 337
    754
    Figure US20100267698A1-20101021-C01404
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-isobutyl- 1H-imidazo[4,5-c]pyridine 1H NMR (CDCl3) δ 9.03 (s, 1 H), 8.39 (d, 1 H), 8.15 (d, 1 H), 7.85 (dd, 1 H), 7.27 (d, 1 H), 7.24 (s, 1 H), 7.17 (s, 1 H), 6.85 (d, 1 H), 6.25 (s, 2 H), 4.08 (d, 2 H), 2.09 (septet, 1 H), 0.85 (d, 6 H) m/e 323
    755
    Figure US20100267698A1-20101021-C01405
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-5-(1,2,4- oxadiazol-3-yl)-1-propyl-1H- benzimidazole Formatc d6-DMSO: 9.62 (s, 1H), 7.99-8.09 (m, 3H), 7.90 (d, J = 8.24 Hz, 1H), 7.79 (d, J = 8.79 Hz, 1H), 7.52 (s, 1H), 7.17 (s, 1H), 7.02 (d, J = 7.41 Hz, 1H), 6.16 (s, 2H), 4.34- 4.39 (m, 2H), 1.7-1.84 (m, 2H), 0.92 (t, J = 7.14 Hz, 3H).
    756
    Figure US20100267698A1-20101021-C01406
         		{(2S)-1-[(2-{[2-(6-fluoropyridin- 2-yl)-1H-imidazol-1-yl]methyl}- 1-propyl-1H-benzimidazol-5-yl) carbonyl]pyrrolidin-2- yl}methanol CDCl3: 8.18 (d, J = 7.69 Hz, 1H), 7.86-7.94 (m, 2H), 7.51 (d, J = 8.52 Hz, 1H). 7.38 (d, J = 8.52 Hz, 1H), 7.22 (s, 1H), 7.17 (s, 1H), 6.9 (dd, J = 2.75. 8.24 Hz, 1H), 6.30 (s, 2H), 4.43-4.46 (m, 1H), 4.23-4.28 (m, 2H), 3.76-3.79 (m, 2H), 3.52-3.62 (m, 2H), 2.16-2.19 (m, 1H), 1.25- 1.85 (m, 5H), 0.82 (t, J = 7.23 Hz, 2H).
    757
    Figure US20100267698A1-20101021-C01407
         		3-(cyclopropylmethyl)-2-{[2-(6- fluoropyridin-2-yl)-1H-imidazol- 1-yl]methyl}-3H-imidazo[4,5- b]pyridine 1H NMR, δ ppm (CDCl3): 8.37(dd, 1H), 8.18(dd, 1H), 8.01(dd, 1H), 7.88(m, 1H), 7.20-7.26(m, 3H), 6.87(dd, 1H), 6.31(s, 2H), 4.31(d, 2H), 1.22(m, 1H), 0.42-0.53(m, 4H) 349.3  [M + 1]
    758
    Figure US20100267698A1-20101021-C01408
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-3-propyl- 3H-imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.38(dd, 1H), 8.19(m, 1H), 8.02(dd, 1H), 7.89(m, 1H), 7.20-7.26(m, 3H), 6.88(m, 1H), 6.32(s, 2H), 4.35(t, 2H), 1.74(m, 2H), 0.85(t, 3H) 337.2  [M + 1]
    759
    Figure US20100267698A1-20101021-C01409
         		5-chloro-3-ethyl-2-{[2-(3- fluorophenyl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine 356.2  [M + 1]
    760
    Figure US20100267698A1-20101021-C01410
         		5-chloro-2-{[2-(2,5- difluorophenyl)-1H-imidazol-1- yl]methyl}-3-ethyl-3H- imidazo[4,5-b]pyridine 1H NMR (CDCl3): 7.96(1H, d), 7.38-7.34(1H, m), 7.26-7.17(4H, m), 7.04(1H, d), 5.37(1H, s), 3.96(2H, q), 1.06(3H, t) 374.2  [M + 1]
    761
    Figure US20100267698A1-20101021-C01411
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-5-(1,3,4- oxadiazol-2-yl)-1-propyl-1H- benzimidazole CDCl3: 8.45 9s, 1H), 8.43 (s, 1H), 8.19 (d, J = 7.14 Hz, 1H), 8.10 (d, J = 8.38 Hz, 1H), 7.90(q, J = 7.96 Hz, 1H), 7.48 (d, J = 8.52 Hz, 1H), 7.19 (s, 1H), 6.89-6.92 (m, 1H), 6.33 (s, 2H), 4.3 (t, J = 7.42 Hz, 2H), 1.67- 1.74 9m, 2H), 0.86 (t, J = 7.42 Hz, 3H) 404.6  [M + 1]; 402.3  [M − 1]
    762
    Figure US20100267698A1-20101021-C01412
         		1-ethyl-2-{[2-(6-fluoropyridin-2- yl)-1H-imidazol-1-yl]methyl}- 1H-imidazo[4,5-c]pyridine 1H NMR (CDCl3) δ 8.82 (s, 1 H), 8.45 (d, 1 H), 8.18 (d, 1 H), 7.88 (dd, 1 H), 7.65 (d, 1 H), 7.23 (d, 1 H), 7.20 (d, 1 H), 6.88 (d, 1 H), 6.30 (s, 2 H), 4.47 (q, 2 H), 1.33 (t, 3 H) m/e 323
    763
    Figure US20100267698A1-20101021-C01413
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-(2- methoxyethyl)-1H-imidazo[4,5- c]pyridine 1H NMR (CDCl3) δ 9,.02 (s, 1 H), 8.41 (d, 1 H), 8.16 (d, 1 H), 7.84 (dd, 1 H), 7.36 (d, 1 H), 7.25 (d, 1 H), 7.21 (d, 1 H), 6.83 (d, 1 H), 6.18 (s, 2 H), 4.57 (t, 2 H), 3.56 (t, 2 H), 3.20 (s, 3 H) m/e 353
    764
    Figure US20100267698A1-20101021-C01414
         		1-ethyl-4-fluoro-2-{[2-(3- fluorophenyl)-1H-imidazol-1- yl]methyl}-1H-imidazo[4,5- c]pyridine 1H NMR (CDCl3): 7.97(1H, dd), 7.53-7.48(1H, m), 7.44-7.38(2H. m), 7.22-7.12(3H. m), 7.04(1H, s), 5.55(2H, s), 3.75(2H, q), 0.96(3H, t) 340.1  [M + 1]
    765
    Figure US20100267698A1-20101021-C01415
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-3-isobutyl- 3H-imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.38(dd, 1H), 8.18(dd, 1H), 8.01(dd, 1H), 7.88(m, 1H), 7.20-7.27(m, 3H), 6.87(dd, 1H), 6.29(s, 2H), 4.21(d, 2H), 2.27(m, 1H), 0.87(d, 6H) 351.3  [M + 1]
    766
    Figure US20100267698A1-20101021-C01416
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-3- isopropyl-3H-imidazo[4,5- b]pyridine 1H NMR, δ ppm (CDCl3): 8.36(dd, 1H), 8.18(m, 1H), 8.00(dd, 1H), 7.90(m, 1H), 7.18-7.22(m, 2H), 7.13(d, 1H), 6.89(m, 1H), 6.32(s, 2H), 5.04(m, 1H), 1.63(d, 6H) 337.2  [M + 1]
    767
    Figure US20100267698A1-20101021-C01417
         		5-chloro-3-ethyl-2-{[2-(6- fluoropyridin-2-yl)-1H-imidazol- 1-yl]methyl}-3H-imidazo[4,5- b]pyridine 1H NMR (CDCl3): 8.17(1H, dd), 7.94-7.84(2H, m), 7.23-7.20(3H, m), 6.89-6.85(1H, m), 6.27(2H, s), 4.45(2H, q), 1.31(3H, t) 357.2  [M + 1]
    768
    Figure US20100267698A1-20101021-C01418
         		5-chloro-2-{[2-(4-chloropyridin-2- yl)-1H-imidazol-1-yl]methyl}-3- ethyl-3H-imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.41(1H, d), 8.35(1H, dd), 7.94(1H, d), 7.26- 7.17(4H, m), 6.36(2H, s), 4.36(2H, q), 1.22(3H, t) 373.2 (M)
    769
    Figure US20100267698A1-20101021-C01419
         		5-chloro-2-{[2-(6-chloropyridin-2- yl)-1H-imidazol-1-yl]methyl}-3- ethyl-3H-imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.22(1H, d), 7.94(1H, d), 7.75(1H, t), 7.29- 7.20(4H, m), 6.31(2H, s), 4.45(2H, q), 1.30(3H, t) 373.3 (M)
    770
    Figure US20100267698A1-20101021-C01420
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-isobutyl- 1H-imidazo[4,5-b]pyridine (HCl salt) H-1 NMR (dmso): 1.05 (d, 6H), 2.40 (h, 1H), 4.50 (d, 2H), 6.57 (s, 2H), 7.30 (d, 1H), 7.82 (m, 1H), 7.93 (s, 1H), 8.03(m, 2H), 8.23(q, 1H), 8.62(d, 1H), 8.95(d, 1H) 351   [M + 1]
    771
    Figure US20100267698A1-20101021-C01421
         		1-(cyclopropylmethyl)-2-{[2-(6- fluoropyridin-2-yl)-1H-imidazol- 1-yl]methyl}-1H-imidazo[4,5- b]pyridine (free base) H-1 NMR (CDCl3): 0.30 (m, 2H), 0.57(m, 2H), 1.05 (m, 1H), 4.23(d, 2H), 6.38(s, 2H), 6.80(d, 1H), 7.08(s, 1H), 7.22 (m, 1H), 7.39(s, 1H), 7.73(d, 1H), 7.90(q, 1H), 8.20(d, 1H), 8.58(d, 1H) 349   [M + 1]
    772
    Figure US20100267698A1-20101021-C01422
         		1-allyl-2-{[2-(6-fluoropyridin-2-yl)- 1H-imidazol-1-yl]methyl}-1H- imidazo[4,5-b]pyridine (HCl) salt Melting point: 225-231 (decompose) H-1 NMR (dmso): 5.22-5.53 (m, 4H), 6.21(m, 1H), 6.53 (s, 2H), 7.24(d, 1H), 7.82 (m, 1H), 7.95(s, 1H), 8.05(m, 2H), 8.25(q, 1H), 8.62(d, 1H), 8.82(d, 1H) 335   [M + 1]
    773
    Figure US20100267698A1-20101021-C01423
         		5-fluoro-2-{[2-(6-fluoropyridin-2- yl)-1H-imidazol-1-yl]methyl}-3- propyl-3H-imidazo[4,5-b]pyridine hydrochloride 1H NMR (d6 DMSO) 8.42(1H, d), 8.26(1H, q), 8.10-8.05(2H, m), 7.96(1H, d), 7.36(1H, dd), 6.97(1H, d), 6.33(2H, s), 4.30(2H, t), 1.90-1.83(2H, m), 0.93(3H, t)
    774
    Figure US20100267698A1-20101021-C01424
         		1-(2-{[2-(2,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl-1H- benzimidazol-5-yl)ethanone 1H NMR, δ ppm (CDCl3): 8.39(dd, 1H), 8.01(dd, 1H), 7.34-7.41(m, 2H), 7.18-7.24(m, 3H), 7.04(d, 1H), 5.40(s, 2H), 3.88(q, 2H), 2.69(s, 3H), 1.03(t, 3H) 381.4  [M + 1]
    775
    Figure US20100267698A1-20101021-C01425
         		methyl 2-{[2-(6-fluoropyridin-2-yl)- 1H-imidazol-1-yl]methyl}-3-propyl- 3H-imidazo[4,5-b]pyridine-6- carboxylate 1H NMR (CDCl3): 9.05(1H, d), 8.62(1H, d), 8.19(1H, dd), 7.88(1H, q), 7.26-7.21(2H, m), 6.89-6.85(1H, m), 6.31(2H, s), 4.39(2H, t), 3.96(3H, s), 1.79-1.72(2H, m), 0.87(3H, t) 395.3  [M + 1]
    776
    Figure US20100267698A1-20101021-C01426
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-6-(1,3,4- oxadiazol-2-yl)-3-propyl-3H- imidazo[4,5-b]pyridine (KC 1084-78-2) 405.3  [M + 1]
    777
    Figure US20100267698A1-20101021-C01427
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-6-(1,3,4- oxadiazol-2-yl)-1-propyl-1H- benzimidazole 1H NMR (CDCl3) δ 8.46 (s, 1 H), 8.18-8.14 (m, 2 H), 7.95-7.83 (m, 3 H), 7.24 (d, 1 H), 7.17 (d, 1 H), 6.87 (d, 1 H), 6.31 (s, 2 H), 4.30 (t, 2 H), 1.71 (q, 2 H), 0.85 (t, 3 H) m/e 404
    778
    Figure US20100267698A1-20101021-C01428
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-6-(5-methyl- 1,3,4-oxadiazol-2-yl)-1-propyl-1H- benzimidazole 1NMR (CDCl3) δ 8.17 (d, 1 H), 8.07 (s, 1 H), 7.91-7.80 (m, 3 H), 7.23 (d, 1 H), 7.16 (d, 1 H), 6.87 (d, 1 H), 6.29 (s, 2 H), 4.28 (t, 2 H), 2.61 (s, 3 H), 1.69 (q, 2 H), 0.84 (t, 3 H) m/e 418
    779
    Figure US20100267698A1-20101021-C01429
         		2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-6-(1,2,4- oxadiazol-3-yl)-1-propyl-1H- benzimidazole 1H NMR (CDCl3) δ 8.89 (s, 1 H), 8.15 (s, 1 H), 8.12-8.03 (m, 1 H), 7.81-7.73 (m, 1 H), 7.40-7.02 (m, 6 H), 5.47 (s, 2 H), 3.82 (t, 2 H), 1.55 (q, 2 H), 0.79 (t, 3 H) m/e 403
    780
    Figure US20100267698A1-20101021-C01430
         		2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-6-(5-methyl- 1,3,4-oxadiazol-2-yl)-1-propyl-1H- benzimidazole 1NMR (CDCl3) δ 8.04 (s, 1 H), 7.94-7.84 (m, 2 H), 7.50 (7.39 (m, 3 H), 7.22-7.16 (m, 2 H), 7.08 (d, 1 H), 5.52 (s, 2 H), 3.76 (t, 2 H), 2.63 (s, 3 H), 1.47 (q, 2 H), 0.77 (t, 3 H) m/e 417
    781
    Figure US20100267698A1-20101021-C01431
         		2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-6-(1,3,4- oxadiazol-2-yl)-1-propyl-1H- benzimidazole 1H NMR (CDCl3) δ 8.50 (s, 1 H), 8.15 (d, 1 H), 7.98-7.89 (m, 2 H), 7.56-7.39 (m, 3 H), 7.24-7.05 (m, 3 H), 5.57 (s, 2 H), 3.78 (t, 2 H), 1.47 (q, 2 H), 0.80 (t, 3 H) m/e 403
    782
    Figure US20100267698A1-20101021-C01432
         		2-{[2-(6-chloropyridin-2-yl)-1H- imidazol-1-yl]methyl}-6-(1,3,4- oxadiazol-2-yl)-3-propyl-3H- imidazo[4,5-b]pyridine 1H NMR (CDCl3): 9.14(1H, d), 8.63(1H, d), 8.51(1H, s), 8.26(1H, d), 7.76(1H, t), 7.28-7.24(3H, m), 6.36(2H, s), 4.42(2H, t), 1.81- 1.74(2H, m), 0.88(3H, t) 421.3  [M + 1]
    783
    Figure US20100267698A1-20101021-C01433
         		2-{[2-(6-chloropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-propyl-1H- benzimidazole-5-carbonitrile 1H NMR (d6 DMSO): 8.02 (1H, d, J = 7.7 Hz), 8.08(1H, s), 7.76(1H, t, J = 8 Hz), 7.55(1H, dd, J = 8, 1.4 Hz), 7.428 (1H, d, J = 8.5 Hz), 7.18- 7.32(3H, m), 6.34(1H, s), 4.29(2H, t, J = 8.7 Hz), 1.66(2H, m), 0.81(3H, t, J = 8.7 Hz) 377.3  [M + 1]
    784
    Figure US20100267698A1-20101021-C01434
         		1-propyl-2-[(2-pyridin-2-yl-1H- imidazol-1-yl)methyl]-1H- benzimidazole-5-carbonitrile
    785
    Figure US20100267698A1-20101021-C01435
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-5-{[(2S)-2- (methoxymethyl)pyrrolidin-1- yl]carbonyl}-1-propyl-1H- benzimidazole CDCl3: 8.17 (d, J = 7.42 Hz, 1H), 7.85-7.93 (m, 2H), 7.51-7.53 (m, 1H), 7.35 (d, J = 8.51 Hz, 1H), 7.21 (s, 1H), 7.16 (s, 1H), 6.89 (dd, J = 2.47, 7.97 Hz, 1H), 6.28-6.3 (m, 2H), 4.46 (bs, 1H), 4.21-4.26 (m, 2H), 3.51-3.68 (m, 4H), 3.40 (s, 3H), 1.95-2.05 (m, 4H), 1.62-1.72 (m, 2H), 0.81 (t, J = 7.42 Hz, 3H). 475.2  [M + 1]; 477.2  [M − 1]
    786
    Figure US20100267698A1-20101021-C01436
         		2-{[2-(4-chloropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-propyl-1H- benzimidazole-5-carbonitrile 1H NMR (d6 DMSO): 8.42 (1H, d, J = 5 Hz), 8.35(1H, s), 8.08(1H, s), 7.1-7.65(1H, m), 6.41(1H, s), 4.19(2H, t, J = 7.4 Hz), 1.57(2H, m), 0.78(3H, t, J = 7.4 Hz) 377.3  [M + 1]
    787
    Figure US20100267698A1-20101021-C01437
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-propyl-5- [(2-pyridin-3-ylpiperidin-1- yl)carbonyl]-1H-benzimidazole CDCl3: 8.61 (s, 1H), 8.52 (d, J = 4.67 Hz, 1H), 8.15-8.18 (m, 1H), 7.87-7.93 (m, 1H), 7.85 (s, 1H), 7.66 (d, J = 7.69 Hz, 1H), 7.30-7.45 (m, 3H), 7.21 (s, 1H), 7.16 (s, 1H), 6.88-6.91 (m, 1H), 6.29 (s, 2H), 4.25 (t, J = 7.42 Hz, 2H), 2.82-2.91 524.0 [M + ], 522.4  [M − 1]
    788
    Figure US20100267698A1-20101021-C01438
         		1-(2-{[2-(4-chloropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-ethyl-1H- benzimidazol-5-yl)ethanone 1H NMR, δ ppm (CDCl3): 8.36- 8.47(m, 3H), 8.01(dd, 1H), 7.38(d, 1H), 7.29(dd, 1H), 7.16-7.18(m, 2H), 6.43(s, 2H), 4.30(q, 2H), 2.68(s, 3H), 1.14(t, 3H) 380.3  [M + 1]
    789
    Figure US20100267698A1-20101021-C01439
         		1-(2-{[2-(6-chloropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-ethyl-1H- benzimidazol-5-yl)ethanone 1H NMR, δ ppm (CDCl3): 8.40(d, 1H), 8.23(d, 1H), 8.01(dd, 1H), 7.77(t, 1H), 7.41(d, 1H), 7.30(d, 1H), 7.24(d, 1H), 7.18(d, 1H), 6.36(s, 2H), 4.41(q, 2H), 2.68(s, 3H), 1.25(t, 3H) 380.3  [M + ]
    790
    Figure US20100267698A1-20101021-C01440
         		2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-6-(5-methyl- 1,2,4-oxadiazol-3-yl)-1-propyl-1H- benzimidazole 1H NMR (CDCl3) δ 8.03 (d, 1 H), 8.02 (s, 1 H), 7.85 (d, 1 H), 7.51- 7.40 (m, 3 H), 7.21-7.16 (m, 2 H), 7.07 (d, 1 H), 5.52 (s, 2 H), 3.75 (t, 2 H), 2.67 (s, 3 H), 1.47 (q, 2 H), 0.76 (t, 3 H) m/e 417
    791
    Figure US20100267698A1-20101021-C01441
         		6-(5-ethyl-1,2,4-oxadiazol-3-yl)-2- {[2-(3-fluorophenyl)-1H-imidazol- 1-yl]methyl}-1-propyl-1H- benzimidazole 1H NMR (CDCl3) δ 8.05 (d, 1 H), 8.03 (s, 1 H), 7.85 (d, 1 H), 7.52- 7.41 (m, 3 H), 7.21-7.17 (m, 2 H), 7.08 (d, 1 H), 5.52 (s, 2 H), 3.76 (t, 2 H), 3.00 (q, 2 H), 1.51-1.45 (m, 5 H), 0.77 (t, 3 H) m/e 431
    792
    Figure US20100267698A1-20101021-C01442
         		2-{[2-(6-chloropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-propyl-1H- imidazo[4,5-c]pyridine dihydrochloride 1H NMR (DMSO- d6) δ 9.32 (s, 1 H), 8.68 (d, 1 H), 8.47 (d, 1 H), 8.45 (d, 1 H), 8.09- 8.03 (m, 2H), 7.80 (d, 1 H), 7.54 (d, 1 H), 6.45 (s, 2 H), 4.61 (t, 2 H), 1.88 (q, 2 H), 0.95 (t, 3 H) m/e 352
    793
    Figure US20100267698A1-20101021-C01443
         		2-{[2-(6-chloropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-ethyl-1H- benzimidazole-5-carbonitrile Mesylate d6-DMSO: 8.02-8.14 (m, 3H), 7.97 (s, 1H), 7.86 (d, J = 8.52 Hz, 1H), 7.77 (s, 1H), 7.6 (d, J = 8.51 Hz, 1H, 7.57 (d, J = 7.97 Hz, 1H), 6.26 (s, 2H), 4.44-4.51 (m, 2H), 2.30 (s, 3H), 1.39 (t, J = 7.14 Hz, 3H) 363.2  [M + 1]
    794
    Figure US20100267698A1-20101021-C01444
         		2-{[2-(4-chloropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-propyl-1H- imidazo[4,5-c]pyridine dihydrochloride 1H NMR (DMSO- d6) δ 9.31 (s, 1 H), 8.66 (d, 1 H), 8.45 (s, 1 H), 8.41 (d, 1 H), 8.32 (d, 1 H), 7.86 (d, 1 H), 7.63 (d, 1 H), 7.52 (d, 1 H), 6.42 (s, 2 H), 4.55 (t, 2 H), 1.91 (q, 2 H), 0.97 (t, 3 H) m/e 353
    795
    Figure US20100267698A1-20101021-C01445
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-6-(5-methyl- 1,3,4-oxadiazol-2-yl)-3-propyl-3H- imidazo[4,5-b]pyridine 1H NMR (CDCl3): 9.07(1H, d), 8.56(1H, d), 8.20(1H, dd), 7.88(1H, q), 7.26-7.2292H, m), 6.88(1H, dd), 6.3292H, s), 4.40(2H, t), 2.65(3H, s), 1.81-1.74(2H, m), 0.88(3H, t) 419.3  [M + 1]
    796
    Figure US20100267698A1-20101021-C01446
         		2-{[2-(2-fluoropyridin-4-yl)-1H- imidazol-1-yl]methyl}-1-propyl-1H- benzimidazole-5-carbonitrile 1H NMR (CDCl3): 8.28(1H, d), 8.04(1H, s), 7.54-7.49(2H, m), 7.40(1H, d), 7.25-7.22(2H, m), 7.11(1H, s), 5.55(2H, s), 3.89(2H, t), 1.60-1.53(2H, m), 0.83(3H, t) 361.2  [M + 1]
    797
    Figure US20100267698A1-20101021-C01447
         		2-{[2-(6-ethoxypyridin-2-yl)-1H- imidazol-1-yl]methyl}-N,N- dimethyl-1-propyl-1H- benzimidazole-5-carboxamide 1H NMR (CDCl3): 8.49(1H, d), 8.10(1H, d), 7.88(1H, d), 7.71(1H, t), 7.13(1H, d), 7.00(1H, d), 6.74(1H, d), 6.45(2H, s), 4.27(2H, q), 4.08(2H, t), 3.15-3.06(6H, br d), 1.48-1.35(5H, m), 0.67(3H, t) 434.6  [M + 1]
    798
    Figure US20100267698A1-20101021-C01448
         		2-{[2-(4-chloropyridin-2-yl)-1H- imidazol-1-yl]methyl}-3-ethyl-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.44(dd, 1H), 8.39(m, 1H), 8.03(dd, 1H), 7.18-7.28(m, 5H), 6.41(s, 2H), 4.39(q, 2H), 1.20(t, 3H) 339.2  [M + 1]
    799
    Figure US20100267698A1-20101021-C01449
         		2-{[2-(6-chloropyridin-2-yl)-1H- imidazol-1-yl]methyl}-3-ethyl-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.39(dd, 1H), 8.23(dd, 1H), 8.03(dd, 1H), 7.76(t, 1H), 7.19-7.29(m, 4H), 6.36(s, 2H), 4.48(q, 2H), 1.29(t, 3H) 339.2  [M + 1]
    800
    Figure US20100267698A1-20101021-C01450
         		3-(2-fluoroethyl)-2-{[2-(3- fluorophenyl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine H-1 NMR (CDCl3): 4.26(t, 1H), 4.35(t, 1H), 4.51(t, 1H), 4.67(t, 1H), 5.54(s, 2H), 7.09-7.45 (m, 4H), 8.06(d, 1H), 8.36(d, 1H) 340   [M + 1]
    801
    Figure US20100267698A1-20101021-C01451
         		3-cyclopropyl-2-{[2-(6- fluoropyridin-2-yl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine H-1 NMR (CDCl3): line list provided 1.20-1.24 (m, 4H), 3.35 (m, 1H), 6.29 (s, 2H), 6.79 (d, 1H), 7.15 (m, 3H), 7.82 (q, 1H), 7.92 (d, 1H), 8.15 (d, 1H), 8.37(d, 1H) 335   [M + 1]
    802
    Figure US20100267698A1-20101021-C01452
         		2-{[2-(6-chloropyridin-2-yl)-1H- imidazol-1-yl]methyl}-3-(2- fluoroethyl)-3H-imidazo[4,5- b]pyridine HCl salt H-1 NMR (dmso): line list provided 4.77 (s, 2H), 4.90 (m, 2H), 6.28 (s, 2H), 7.24 (m, 1H), 7.62 (d, 1H), 7.93 (m, 2H), 8.12 (m, 2H), 8.36(m, 2H) 357   [M + 1]
    803
    Figure US20100267698A1-20101021-C01453
         		1-(2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-3-propyl-3H- imidazo[4,5-b]pyridin-6- yl)ethanone 1H NMR (CDCl3): 9.01(1H, d), 8.53(1H, d), 8.20-8.17(1H, m), 7.87(1H, q), 7.24-7.21(2H, m), 6.88- 6.84(1H, m), 6.30(2H, s), 4.40(2H, t), 2.66(3H, s), 1.80-1.73(2H, m), 0.88(3H, t) 379.3  [M + 1]
    804
    Figure US20100267698A1-20101021-C01454
         		5-chloro-2-{[2-(6-fluoropyridin-2- yl)-1H-imidazol-1-yl]methyl}-3- propyl-3H-imidazo[4,5-b]pyridine hydrochloride 1H NMR (d6 DMSO): 8.35(1H, dd), 8.24(1H, q), 8.02(1H, s), 7.95(1H, dd), 7.90(1H, s), 7.33(1H, dd), 7.28(1H, d), 7.26(1H, d), 6.32(2H, s), 4.33(2H, t), 1.90-1.85(2H, m), 0.94(3H, t) 371.3  [M + 1]
    805
    Figure US20100267698A1-20101021-C01455
         		2-{[2-(3-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-3-propyl-3H- imidazo[4,5-b]pyridine dihydrochloride 1H NMR (d6 DMSO): 8.48-8.46(1H, m), 8.30(1H, dd), 8.12-8.03(2H, m), 7.96(1H, d), 7.90(1H, dd), 7.72-7.65(1H, m), 7.22(1H, dd), 6.18(2H, s), 4.27(2H, t), 1.83-1.75(2H, m), 0.87(3H, t) 337.2 
    806
    Figure US20100267698A1-20101021-C01456
         		2-{[2-(3-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-propyl-1H- benzimidazole-5-carbonitrile hydrochloride 1H NMR (d6 DMSO): 8.45(1H, m), 8.08-8.02(3H, m), 7.93(1H, s), 7.83(1H, d), 7.69- 7.62(2H, m), 6.16(2H, s), 4.29(2H, t), 1.72-1.20(2H, m), 0.86(3H, t) 361.2  [M + 1]
    807
    Figure US20100267698A1-20101021-C01457
         		6-{1-[(1-propyl-1H-imidazo[4,5- c]pyridin-2-yl)methyl]-1H- imidazol-2-yl}pyridine-2- carbonitrile 1H NMR (CDCl3) δ 9.03 (s, 1 H), 8.56 (d, 1 H), 8.44 (d, 1 H), 7.91 (dd, 1 H), 7.60 (d, 1 H), 7.32 (d, 1 H), 7.30 (d, 1 H), 7.25 (d, 1 H), 6.25 (s, 2 H), 4.331 (t, 2 H), 1.78 (q, 2 H), 0.90 (t, 3 H) m/e 344
    808
    Figure US20100267698A1-20101021-C01458
         		2-{[2-(4-chloropyridin-2-yl)-1H- imidazol-1-yl]methyl}-3-propyl-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.36- 8.45(m, 3H), 8.03(dd, 1H), 7.18- 7.29(m, 4H), 6.40(s, 2H), 4.28(t, 2H), 1.66(m, 2H), 0.81(t, 3H)
    809
    Figure US20100267698A1-20101021-C01459
         		2-{[2-(6-chloropyridin-2-yl)-1H- imidazol-1-yl]methyl}-3-propyl-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.39(dd, 1H), 8.23(dd, 1H), 8.02(dd, 1H), 7.76(t, 1H), 7.19-7.29(m, 4H), 6.36(s, 2H), 4.34(t, 2H), 1.72(m, 2H), 0.83(t, 3H)
    810
    Figure US20100267698A1-20101021-C01460
         		2-{[2-(4-chloropyridin-2-yl)-1H- imidazol-1-yl]methyl}-3- cyclopropyl-3H-imidazo[4,5- b]pyridine HCl salt H-1 NMR (DMSO): line list provided 1.24 (m, 4H), 3.48 (m, 1H), 6.43 (s, 2H), 7.19 (m, 1H), 7.67 (m, 1H), 7.86 (d, 1H), 7.97 (s, 1H), 8.06(s, 1H), 8.31 (d, 1H), 8.56 (d, 1H), 8.64 (m, 1H) 351   [M + 1]; 349   [M − 1]
    811
    Figure US20100267698A1-20101021-C01461
         		2-{[2-(4-chloropyridin-2-yl)-1H- imidazol-1-yl]methyl}-3-(2- fluoroethyl)-3H-imidazo[4,5- b]pyridine H-1 NMR (CDCl3): 4.60-4.77 (m, 2H), 4.81 (s, 2H), 6.35(s, 2H), 7.20- 7.25 (m, 5H), 8.03 (d, 1H), 8.38 (m, 1H) 357   [M + 1]
    812
    Figure US20100267698A1-20101021-C01462
         		6-{1-[(3-propyl-3H-imidazo[4,5- b]pyridin-2-yl)methyl]-1H- imidazol-2-yl}pyridine-2- carbonitrile 1H NMR, dppm (CDCl3): 8.56(dd, 1H), 8.39(dd, 1H), 8.00(dd, 1H), 7.92(dd, 1H), 7.63(dd, 1H), 7.20- 7.29(m, 3H), 6.32(s, 2H), 4.36(t, 2H), 1.80(m, 2H), 0.89(t, 3H), LC- MS: calcd: 343.39, found 344.1[M + 1]
    813
    Figure US20100267698A1-20101021-C01463
         		6-{1-[(3-propyl-3H-imidazo[4,5- b]pyridin-2-yl)methyl]-1H- imidazol-2-yl}pyridine-2- carboxamide 1H NMR, δ ppm (CDCl3): 8.88(s, 1H), 8.42(dd, 1H), 8.38(dd, 1H), 8.15(dd, 1H), 7.98(dd, 1H), 7.93(d, 1H), 7.22-7.27(m, 3H), 6.18 (s, 2H), 5.67(s, 1H), 4.07(t, 2H), 1.54(m, 2H), 0.83(t, 3H) 362.2  [M + 1]
    814
    Figure US20100267698A1-20101021-C01464
         		3-propyl-2-({2-[6- (trifluoromethyl)pyridin-2-yl]-1H- imidazol-1-yl}methyl)-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.53(d, 1H), 8.39(dd, 1H), 7.96-8.06(m, 2H), 7.64(dd, 1H), 7.20-7.27(m, 3H), 6.42(s, 2H), 4.26(t, 2H), 1.61(m, 2H), 0.72(t, 3H) 387.3  [M + 1]
    815
    Figure US20100267698A1-20101021-C01465
         		3-(2-fluoroethyl)-2-{[2-(3- fluoropyridin-2-yl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine H-1 NMR (CDCl3): line list provided 4.58-4.66 (m, 2H), 4.75 (s, 2H), 6.03 (s, 2H), 7.21-7.35 (m, 4H), 7.57 (m, 1H), 8.04 (d, 1H), 8.41(m, 1H) 341   [M + 1]
    816
    Figure US20100267698A1-20101021-C01466
         		2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-1-phenyl- 1H-benzimidazole H-1 NMR (CDCl3): 5.38(s, 2H), 6.91-7.17(m, 8H), 7.22-7.45 (m, 6H), 7.84 (d, 1H) 369  
    817
    Figure US20100267698A1-20101021-C01467
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-phenyl- 1H-benzimidazole H-1 NMR: 6.22 (s, 2H), 6.78 (m, 1H), 7.03-7.37 (m, 9H), 7.65-7.92 (m, 3H) 368   [M − 1]
    818
    Figure US20100267698A1-20101021-C01468
         		3-allyl-2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-3H- imidazo[4,5-b]pyridine H-1 NMR (CDCl3): 4.59-4.62 (m, 3H), 5.03 (d, 1H), 5.43(s, 2H), 5.70 (m, 1H), 7.03 (s, 1h), 7.17 (m, 1H), 7.25 (m, 2H), 7.43(m, 3H), 8.04 (d, 1H), 8.40 (d, 1H) 334   [M + 1]; 332   [M − 1]
    819
    Figure US20100267698A1-20101021-C01469
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-propyl-5- [(4-pyridin-2-ylpiperazin-1- yl)carbonyl]-1H-benzimidazole CDCl3: 8.15-8.2 (m, 2H), 7.82-7.94 (m, 2H), 7.44-7.53 (m, 1H), 7.35- 7.44 (m, 2H), 7.18 (q, 2H), 6.88 (d, 1H), 6.71 (s, 1H), 6.65 (s, 1H), 6.29 (s, 2H), 4.21-4.29 (m, 2H), 3.53-4.0 (m, 8H), 1.53-1.71 (m, 2H), 0.82 (t, 3H)
    820
    Figure US20100267698A1-20101021-C01470
         		5-[(2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-propyl-1H- benzimidazol-5-yl)carbonyl]-2- oxa-5-azabicyclo[2.2.1]heptane CDCl3: 8.18 (d, 7.97 Hz, 1 Hz), 7.85 7.94 (m, 2H), 7.52-7.54 (m, 1H), 7.38-7.42 (m, 1H), 7.17-7.26 (m, 2H), 6.90 (d, J = 7.96 Hz, 1H), 6.30 (s, 2H), 4.73-5.06 (m, 1H), 4.56 (d, 1H), 4.24-4.35 (m, 2H), 4.04-4.10 (m, 1H), 3.81-3.89 (m, 1H), 3.51-3.7 (m, 2H, 1.96 (s, 1H), 1.86 (s, 1H), 1.64-1.71 (m, 2H), 0.83 (t, J = 7.28 Hz, 3H).
    821
    Figure US20100267698A1-20101021-C01471
         		2-{[2-(2-fluorophenyl)-1H- imidazol-1-yl]methyl}-3-propyl-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.39(dd, 1H), 8.03(dd, 1H), 7.65(m, 1H), 7.50(m, 1H), 7.20-7.35(m, 3H), 7.07(d, 1H), 5.38 (s, 2H), 3.84(t, 2H), 1.46(m, 2H), 0.74(t, 3H) 336.2  [M + 1]
    822
    Figure US20100267698A1-20101021-C01472
         		2-{[2-(2-chlorophenyl)-1H- imidazol-1-yl]methyl}-3-propyl-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3). 8.38(dd, 1H), 8.02(dd, 1H), 7.33-7.56(m, 4H), 7.19-7.27(m, 2H), 7.09(d, 1H), 5.30 (s, 2H), 3.85(t, 2H), 1.50(m, 2H), 0.76(t, 3H) 352.2  [M + 1]
    823
    Figure US20100267698A1-20101021-C01473
         		3-propyl-2-({2-[2- (trifluoromethyl)phenyl]-1H- imidazol-1-yl}methyl)-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.39(dd, 1H), 8.02(dd, 1H), 7.84 (dd, 1H), 7.53-7.65(m, 2H), 7.45(m, 1H), 7.21- 7.27(m, 2H), 7.09(d, 1H), 5.17 (s, 2H), 3.93(t, 2H), 1.59(m, 2H), 0.80(t, 3H) 386.2  [M + 1]
    824
    Figure US20100267698A1-20101021-C01474
         		3-ethyl-2-{[2-(3-fluoropyridin-2-yl)- 1H-imidazol-1-yl]methyl}-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.47(m, 1H), 8.40(dd, 1H), 8.04(dd, 1H), 7.62(m, 1H), 7.36(m, 1H), 7.23- 7.28(m, 2H), 7.16(d, 1H), 6.10(s, 2H), 4.37(q, 2H), 1.19(t, 3H) 323.3  [M + 1]
    825
    Figure US20100267698A1-20101021-C01475
         		3-ethyl-2-{[2-(3-fluoropyridin-2-yl)- 1H-imidazol-1-yl]methyl}-6-(5- methyl-1,3,4-oxadiazol-2-yl)-3H- imidazo[4,5-b]pyridine 1H NMR (CDCl3): 9.08(1H, d), 8.58(1H, d), 8.45-8.43(1H, m), 7.65- 7.59(1H, m), 7.39-7.30(2H, m), 7.18(1H, s), 6.14(2H, s), 4.43(2H, q), 2.66(3H, s), 1.23(3H, t) 405.3  [M + 1]
    826
    Figure US20100267698A1-20101021-C01476
         		1-allyl-2-{[2-(6-fluoropyridin-2-yl)- 1H-imidazol-1-yl]methyl}-1H- imidazo[4,5-c]pyridine 1H NMR (CDCl3) δ 9.05 (s, 1 H), 8.40 (d, 1 H), 8.14 (d, 1 H), 7.85 (dd, 1 H), 7.26 (d, 1 H), 7.22 (d, 1 H), 7.17 (6.85 (d, 1 H), 6.19 (s, 2 H), 5.82-5.70 (m, 1 H), 5.12 (d, 1 H), 4.99-4.96 (m, 2H), 4.82 (d, 1 H) m/e 335
    827
    Figure US20100267698A1-20101021-C01477
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-prop-2- ynyl-1H-imidazo[4,5-c]pyridine 1H NMR (CDCl3) δ 9.04 (s, 1 H), 8.46 (d, 1 H), 8.15 (d, 1 H), 7.86 (dd, 1 H), 7.41 (d, 1 H), 7.26 (d, 1 H), 7.21 (d, 1 H), 6.85 (d, 1 H), 6.23 (s, 2 H), 5.21 (d, 2 H), 2.33-2.32 (m, 1 H) m/e 333
    828
    Figure US20100267698A1-20101021-C01478
         		2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-3-prop-2- ynyl-3H-imidazo[4,5-b]pyridine H-1 NMR (dmso): 3.47 (s, 1H), 4.78 (s, 2H), 5.65 (s, 2H), 7.07-7.45 (m, 5H), 7.45(m, 2H), 8.07(m, 1H), 8.42(m, 1H) 332   [M + 1]; 330   [M − 1]
    829
    Figure US20100267698A1-20101021-C01479
         		2-{[2-(3-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-3-prop-2- ynyl-3H-imidazo[4,5-b]pyridine HCl salt H-1 NMR (dmso): line list provided 3.51 (s, 1H), 5.25 (s, 2H), 6.25(s, 2H), 7.27(m, 1H), 7.72(m, 1H), 7.94-8.15 (m, 4H), 8.35(m, 1H), 8.50(1H) 333   [M + 1]
    830
    Figure US20100267698A1-20101021-C01480
         		3-cyclopropyl-2-{[2-(3- fluoropyridin-2-yl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine HCl salt H-1 NMR (dmso): line list provided 1.19 (m, 4H), 3.36 (m, 2H), 6.24 (s, 2H), 7.21 (m, 1H), 7.70 (m, 1H), 7.88 (d, 2H), 8.00 (s, 1H), 8.07 (m, 3H), 8.31 (d, 1H), 8.52 (d, 1H) 335   [M + 1]
    831
    Figure US20100267698A1-20101021-C01481
         		3-cyclopropyl-2-{[2-(3- fluorophenyl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine HCl salt H-1 NMR (DMSO): line list provided 1.03-1.22 (m, 4H), 3.37 (m, 1H), 5.96 (s, 2H), 7.22 (m, 1H), 7.45-7.78 (m, 4H), 7.95-8.03 (m, 3H), 8.37 (d, 1H) 334   [M + 1]; 332   [M − 1]
    832
    Figure US20100267698A1-20101021-C01482
         		3-ethyl-2-({2-[6- (trifluoromethyl)pyridin-2-yl]-1H- imidazol-1-yl}methyl)-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.52(d, 1H), 8.39(dd, 1H), 7.90-8.05(m, 2H), 7.64(d, 1H), 7.21-7.27(m, 3H), 6.42(s, 2H), 4.39(q, 2H), 1.17(t, 3H) 373.3  [M + 1]
    833
    Figure US20100267698A1-20101021-C01483
         		1-[1-ethyl-2-({2-[6- (trifluoromethyl)pyridin-2-yl]-1H- imidazol-1-yl}methyl)-1H- benzimidazol-5-yl]ethanone 1H NMR, δ ppm (CDCl3): 8.52(d, 1H), 8.40(d, 1H), 7.99-8.03(m, 2H), 7.64(d, 1H), 7.40(d, 1H), 7.20- 7.22(m, 2H), 6.41(s, 2H), 4.34(q, 2H), 2.68(s, 3H), 1.15(t, 3H) 414.2  [M + 1]
    834
    Figure US20100267698A1-20101021-C01484
         		3-(2-fluoroethyl)-2-({2-[6- (trifluoromethyl)pyridin-2-yl]-1H- imidazol-1-yl}methyl)-3H- imidazo[4,5-b]pyridine H-1 NMR (CDCl3): line list provided 4.60-4.81 (m, 4H), 6.30 (s, 2H), 7.18-7.30 (m, 3H), 7.54 (d, 1H), 7.88-8.00 (m, 2H), 8.33(d, 1H), 8.49(d, 1H) 391   [M + 1]; 389   [M − 1]
    835
    Figure US20100267698A1-20101021-C01485
         		3-allyl-2-{[2-(6-fluoropyridin-2-yl)- 1H-imidazol-1-yl]methyl}-3H- imidazo[4,5-b]pyridine H-1 NMR (CDCl3): line list provided 4.87 (d, 1H), 5.14 (m, 3H), 5.87 (m, 1H), 6.24 (s, 2H), 6.86 (dd, 1H), 7.23 (m, 3H), 7.84 (m, 1H), 8.02 (d, 1H), 8.15 (m, 1H), 8.38 (d, 1H) 335   [M + 1]; 333   [M − 1]
    836
    Figure US20100267698A1-20101021-C01486
         		6-{1-[(3-ethyl-3H-imidazo[4,5- b]pyridin-2-yl)methyl]-1H- imidazol-2-yl}pyridine-2- carbonitrile 1H NMR, δ ppm (CDCl3): 8.56(dd, 1H), 8.40(dd, 1H), 8.01(dd, 1H), 7.93(t, 1H), 7.63(dd, 1H), 7.21- 7.29(m, 3H), 6.33(s, 2H), 4.44(q, 2H), 1.35(t, 3H) 330.1  [M + 1]
    837
    Figure US20100267698A1-20101021-C01487
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-N,N- dimethyl-1-propyl-1H- benzimidazole-6-carboxamide 1H NMR (CDCl3) δ 8.17 (d, 1 H), 7.89 (dd, 1 H), 7.74 (d, 1 H), 7.49 (s, 1 H), 7.30 (d, 1 H), 7.21 (d, 1 H), 7.15 (d, 1 H), 6.89 (d, 1 H), 6.29 (s, 2 H), 4.23 (t, 2 H), 3.07 (br d, 6 H), 1.66 (q, 2 H), 0.81 (t, 3 H) m/e 407
    838
    Figure US20100267698A1-20101021-C01488
         		1-propyl-2-({2-[6- (trifluoromethyl)pyridin-2-yl]-1H- imidazol-1-yl}methyl)-1H- imidazo[4,5-c]pyridine 1H NMR (CDCl3) δ 9.08 (s, 1 H), 8.52 (d, 1 H), 8.44 (d, 1 H), 7.99 (dd, 1 H), 7.65 (d, 1 H), 7.29 (d, 1 H), 7.26 (d, 1 H), 7.21 (d, 1 H), 6.40 (s, 2 H), 4.19 (t, 2 H), 1.54 (q, 2 H), 0.71 (t, 3 H) m/e 387
    839
    Figure US20100267698A1-20101021-C01489
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-6- (morpholin-4-ylcarbonyl)-1-propyl- 1H-benzimidazole 1H NMR (CDCl3) δ 8.17 (d, 1 H), 7.88 (dd, 1 H), 7.75 (d, 1 H), 7.51 (s, 1 H), 7.28-7.25 (m, 1 H), 7.21 (s, 1 H), 7.16 (s, 1 H), 6.89 (d, 1 H), 6.29 (s, 2 H), 4.24 (t, 2 H), 3.69 (br s, 8 H), 1.67 (q, 2 H), 0.82 (t, 3 H) m/e 449
    840
    Figure US20100267698A1-20101021-C01490
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-propyl-6- [(4-pyridin-2-ylpiperazin-1- yl)carbonyl]-1H-benzimidazole 1H NMR (CDCl3) δ 8.19-8.16 (m, 2 H), 7.88 (dd, 1 H), 7.77 (d, 1 H), 7.53 (s, 1 H), 7.48 (d, 1 H), 7.32 (d, 1 H), 7.26 (d, 1 H), 7.17 (d, 1 H), 6.89 (d, 1 H), 6.69-6.65 (m, 2 H), 6.30 (s, 2 H), 4.25 (t, 2 H), 3.59 (br s, 8 H) 1.67 (q, 2 H), 0.82 (t, 3 H) m/e 525
    841
    Figure US20100267698A1-20101021-C01491
         		methyl 2-{[2-(6-fluoropyridin-2-yl)- 1H-imidazol-1-yl]methyl}-1-propyl- 1H-benzimidazole-6-carboxylate 1H NMR (CDCl3) δ 8.18 (d, 1 H), 8.09 (s, 1 H), 7.98-7.87 (m, 2 H), 7.76 (d, 1 H), 7.24 (s, 1 H), 7.17 (s, 1 H), 6.88 (d, 1 H), 6.31 (s, 2 H), 4.29 (t, 2 H), 3.94 (s, 3 H), 1.69 (q, 2 H), 0.85 (t, 3 H)4. m/e 39
    842
    Figure US20100267698A1-20101021-C01492
         		1-(2-fluoroethyl)-2-{[2-(3- fluorophenyl)-1H-imidazol-1- yl]methyl}-1H-imidazo[4,5- c]pyridine H-1 NMR (CDCl3): 4.02 (t, 1H), 4.05 (t, 1H), 4.32 (t, 1H), 4.47 (t, 1H), 5.58 (s, 2H), 7.05-7.33 (m, 5H), 7.44 (m, 2H), 8.47 (d, 1H), 9.10 (s, 1H) 340   [M + 1]
    843
    Figure US20100267698A1-20101021-C01493
         		2-{[2-(4-chloro-1-methyl-1H- pyrazol-3-yl)-1H-imidazol-1- yl]methyl}-3-(2-fluoroethyl)-3H- imidazo[4,5-b]pyridine H-1 NMR (CDCl3): line list provided 3.89 (s, 3H), 4.55 (s, 2H), 4.69 (m, 1H), 4.71 (m, 1H), 5.93 (s, 2H), 7.11 (s, 1H), 7.25 (m, 2H), 7.47 (s, 1H), 8.04 (d, 1H), 8.36 (d, 1H) 360   [M + 1]
    844
    Figure US20100267698A1-20101021-C01494
         		(4S)-5-[(2-{[2-(6-fluoropyridin-2- yl)-1H-imidazol-1-yl]methyl}-1- propyl-1H-benzimidazol-6- yl)carbonyl]-2-oxa-5- azabicyclo[2.2.1]heptane 1H NMR (CDCl3) δ 8.18 (d, 1 H), 7.89 (dd, 1 H), 7.78-7.72 (m, 1 H), 7.62 (s, 1 H), 7.42-7.33 (m, 1 H), 7.22 (s, 1 H), 7.17 (s, 1 H), 6.89 (d, 1 H), 6.30 (s, 2 H), 5.06-4.51 (m, 2 H), 4.25 (t, 2H), 4.11-3.48 (m, 4 H), 1.96-1.87 (m, 2 H), 1.68 (q, 2 H), 0.83 (t, 3 H) m/e 461
    845
    Figure US20100267698A1-20101021-C01495
         		1-propyl-2-[(2-pyrazin-2-yl-1H- imidazol-1-yl)methyl]-1H- imidazo[4,5-c]pyridine 1H NMR (CDCl3) δ 9.58 (s, 1 H), 9.09 (d, 1 H), 8.61-8.42 (m, 3 H), 7.32-7.24 (m, 3 H), 6.33 (s, 2 H), 4.16 (t, 2 H), 1.63 (q, 2 H), 0.81 (t, 3 H) m/e 320
    846
    Figure US20100267698A1-20101021-C01496
         		3-ethyl-2-{[2-(2-methyl-1,3- thiazol-4-yl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine 1H NMR, δ ppm (CDCl3): 8.40(dd, 1H), 8.05(dd, 1H), 7.88(s, 1H), 7.25(m, 1H), 7.11(d, 1H), 7.07(d, 1H), 6.29(s, 2H), 4.35(q, 2H), 2.78(s, 3H), 1.18(t, 3H) 325.2  [M + 1]
    847
    Figure US20100267698A1-20101021-C01497
         		1-(1-ethyl-2-{[2-(2-methyl-1,3- thiazol-4-yl)-1H-imidazol-1- yl]methyl}-1H-benzimidazol-5- yl)ethanone 1H NMR, δ ppm (CDCl3): 8.41(d, 1H), 8.01(dd, 1H), 7.89(s, 1H), 7.38 (d, 1H), 7.10(d, 1H), 7.05(d, 1H), 6.30(s, 2H), 4.27(q, 2H), 2.79(s, 3H), 2.69(s, 3H), 1.12(t, 3H) 366.2  [M + 1]
    848
    Figure US20100267698A1-20101021-C01498
         		2-{[2-(3,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-3-propyl-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.41(dd, 1H), 8.06(dd, 1H), 7.19-7.29(m, 4H), 7.08(d, 1H), 6.93(m, 1H), 5.52(s, 2H), 3.96(t, 2H), 1.57(m, 3H), 0.82(t, 3H) 354.3  [M + 1]
    849
    Figure US20100267698A1-20101021-C01499
         		2-{[2-(3,5-difluorophenyl)-1H- imidazol-1-yl]methyl}-3-ethyl-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.41(dd, 1H), 8.06(dd, 1H), 7.19-7.31(m, 4H), 7.07(d, 1H), 6.93(m, 1H), 5.52(s, 2H), 4.05(q, 2H), 1.14(t, 3H). 340.3  [M + 1]
    850
    Figure US20100267698A1-20101021-C01500
         		3-propyl-2-{[2-(1,3-thiazol-2-yl)- 1H-imidazol-1-yl]methyl}-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.40(dd, 1H), 8.05(dd, 1H), 7.86(d, 1H), 7.40(d, 1H), 7.25(d, 1H), 7.22(d, 1H), 7.15(d, 1H), 6.37(s, 2H), 4.27(t, 2H), 1.62(m, 2H), 0.78(t, 3H). 325.3  [M + 1]
    851
    Figure US20100267698A1-20101021-C01501
         		3-propyl-2-{[2-(2,4,5- trifluorophenyl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine 1H NMR, δ ppm (CDCl3): 8.41(dd, 1H), 8.03(dd, 1H), 7.50(m, 1H), 7.22-7.29(m, 2H), 7.07-7.15(m, 2H), 5.37(s, 2H), 3.93(t, 2H), 1.53(m, 2H), 0.82(t, 3H). 372.2  [M + 1]
    852
    Figure US20100267698A1-20101021-C01502
         		2-{[2-(6-fluoropyridin-3-yl)-1H- imidazol-1-yl]methyl}-3-propyl-3H- imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.53-8.51(1H, m), 8.40(1H, dd), 8.16-8.14(1H, m), 8.03(1H, dd), 7.28-7.23(2H, m), 7.11(1H, d), 7.05(1h, dd), 5.46(2H, s), 4.00(2H, t), 1.65-1.59(2H, m), 0.84(3H, t) 337.1  [M + 1]
    853
    Figure US20100267698A1-20101021-C01503
         		2-{[2-(1,5-dimethyl-1H-pyrazol-3- yl)-1H-imidazol-1-yl]methyl}-3- propyl-3H-imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.37(1H, dd), 8.04(1H, dd), 7.22(1H, dd), 7.06(1H, d), 7.02(1H, d), 6.66(1H, s), 6.20(1H, s), 4.19(2H, t), 3.83(3H, s), 2.33(3H, s), 1.60-1.53(2H, m), 0.76(3H, t) 336.2  [M + 1]
    854
    Figure US20100267698A1-20101021-C01504
         		2-{[2-(3,4-difluorophenyl)-1H- imidazol-1-yl]methyl}-3-propyl-3H- imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.38(1H, dd), 8.04(1H, dd), 7.56-7.50(1H, m), 7.41-7.36(1H, m), 7.30-7.21(2H, m), 7.15(1H, d), 7.04(1H, d), 5.4592H, s), 3.92(2H, t), 1.60-1.50(2H, m), 0.80(3H, t) 354.3  [M + 1]
    855
    Figure US20100267698A1-20101021-C01505
         		3-(2-fluoroethyl)-2-{[2-(3-fluoro-2- methylphenyl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine H-1 NMR (CDCl3): 2.13 (s, 3H), 4.14 (m, 1H), 4.24 (m, 1H), 4.48 (m, 1H), 4.64 (m, 1H), 5.29 (s, 2H), 7.09 7.26 (m, 6H), 8.02 (d, 1H), 8.35 (d, 1H) 354   [M + 1]
    856
    Figure US20100267698A1-20101021-C01506
         		2-{[2-(2,6-difluorophenyl)-1H- imidazol-1-yl]methyl}-3-(2- fluoroethyl)-3H-imidazo[4,5- b]pyridine H-1 NMR (CDCl3): line list provided 4.14 (t, 1H), 4.24 (t, 1H), 4.46 (t, 1H), 4.64 (t, 1H), 5.40 (s, 2H), 6.98 (m, 2H), 7.11 (s, 1H), 7.26 7.40 (m, 3H), 8.01 (d, 1H), 8.34 (d, 1H) 358   [M + 1]
    857
    Figure US20100267698A1-20101021-C01507
         		3-(2-fluoroethyl)-2-{[2-(3-fluoro-4- methylphenyl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine H-1 NMR (CDCl3): line list provided 2.35 (s, 3H), 4.26 (t, 1H), 4.35 (t, 1H), 4.51 (t, 1H), 4.67 (t, 1H), 5.53 (s, 2H), 7.07 (s, 1H), 7.18 (s, 1H), 7.26-7.34 (m, 4H), 8.07 (d, 1H), 8.36 (d, 1H) 354   [M + 1]
    858
    Figure US20100267698A1-20101021-C01508
         		3-ethyl-2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-6-(5-methyl- 1,3,4-oxadiazol-2-yl)-3H- imidazo[4,5-b]pyridine dihydrochloride 1H NMR (d6 DMSO): 9.96(1H, d), 8.50(1H, d), 7.98-7.91(2H, m), 7.72-7.50(4H, m), 6.01(2H, s), 4.35(2H, q), 2.58(3H, s), 1.35(3H, t) 404.3  [M + 1]
    859
    Figure US20100267698A1-20101021-C01509
         		2-{[2-(2-methyl-1,3-thiazol-4-yl)- 1H-imidazol-1-yl]methyl}-3-propyl- 3H-imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.40(dd, 1H), 8.05(dd, 1H), 7.91(s, 1H), 7.24(m, 1H), 7.11(d, 1H), 7.07(d, 1H), 6.30(s, 2H), 4.22(t, 2H), 2.78(s, 3H), 1.62(m, 2H), 0.79(t, 3H) 339.2  [M + 1]
    860
    Figure US20100267698A1-20101021-C01510
         		2-{[2-(2-methoxypyridin-3-yl)-1H- imidazol-1-yl]methyl}-3-propyl-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.38(dd, 1H), 8.30(dd, 1H), 8.03(dd, 1H), 7.87(dd, 1H), 7.19-7.27(m, 2H), 7.01-7.05(m, 2H), 5.33(s, 2H), 3.97(s, 3H), 3.86(t, 2H), 1.47(m, 2H), 0.77(t, 3H) 349.3  [M + 1]
    861
    Figure US20100267698A1-20101021-C01511
         		2-{[2-(2,3-dichlorophenyl)-1H- imidazol-1-yl]methyl}-3-propyl-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.38(dd, 1H), 8.02(dd, 1H), 7.59(dd, 1H), 7.39(dd, 1H), 7.21-7.29(m, 3H), 7.10(d, 1H), 5.29(s, 2H), 3.90 (t, 2H), 1.53 (m, 2H), 0.79(t, 3H). 386.3 
    862
    Figure US20100267698A1-20101021-C01512
         		2-{[2-(2-fluoropyridin-3-yl)-1H- imidazol-1-yl]methyl}-3-propyl-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.39(dd, 1H), 8.35(m, 1H), 8.12(m, 1H), 8.02(dd, 1H), 7.35(m, 1H), 7.22- 7.29(m, 2H), 7.10(d, 1H), 5.43(s, 2H), 3.95 (t, 2H), 1.54 (m, 2H), 0.80(t, 3H). 337.2 
    863
    Figure US20100267698A1-20101021-C01513
         		2-{[2-(2,3-difluorophenyl)-1H- imidazol-1-yl]methyl}-3-ethyl-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.40(dd, 1H), 8.04(dd, 1H), 7.22-7.43(m, 5H), 7.08(d, 1H), 5.40(s, 2H), 3.99 (q, 2H), 1.05(t, 3H). 340.1 
    864
    Figure US20100267698A1-20101021-C01514
         		2-{[2-(2,3-difluorophenyl)-1H- imidazol-1-yl]methyl}-3-propyl-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.39(dd, 1H), 8.03(dd, 1H), 7.22-7.42(m, 5H), 7.09(d, 1H), 5.39(s, 2H), 3.86(t, 2H), 1.50(m, 2H), 0.78(t, 3H) 354.2 
    865
    Figure US20100267698A1-20101021-C01515
         		2-{[2-(4-chloro-1-methyl-1H- pyrazol-3-yl)-1H-imidazol-1- yl]methyl}-3-propyl-3H- imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.38(1H, dd), 8.04(1H, dd), 7.52(1H, s), 7.26- 7.21(2H, m), 7.05(1H, d), 5.98(2H, s), 4.15(2H, t), 3.94(3H, s), 1.62- 1.52(2H, m), 0.80(3H, t) 356.2  [M + 1]
    866
    Figure US20100267698A1-20101021-C01516
         		3-cyclobutyl-2-55 [2-(6- fluoropyridin-2-yl)-1H-imidazol- 1-yl]methyl}-3H-imidazo[4,5- b]pyridine dihydrochloride 1H NMR (d6 DMSO): 8.39(1H, dd), 8.33(1H, dd), 8.25(1H, q), 8.03(1H, d), 7.95(1H, d), 7.87(1H, dd), 7.33(1H, dd), 7.21(1H, dd), 6.34(2H, s), 5.18(1H, m), 3.28-1.20(2H, m), 2.58-2.42(2H, m), 2.00-1.86(2H, m) 349.3  [M + 1]
    867
    Figure US20100267698A1-20101021-C01517
         		2-{1-[(3-propyl-3H-imidazo[4,5- b]pyridin-2-yl)methyl]-1H- imidazol-2-yl}isonicotinonitrile dihydrochloride 1H NMR (d6 DMSO): 8.78(1H, s), 8.72(1H, d), 8.32(1H, dd), 7.99(1H, d), 7.93- 7.86(3H, m), 7.21(1H, dd), 6.34(2H, s), 4.37(2H, t), 1.91-1.82(2H, m), 0.93(3H, t) 344.2  [M + 1]
    868
    Figure US20100267698A1-20101021-C01518
         		2-{1-[(1-propyl-1H-imidazo[4,5- c]pyridin-2-yl)methyl]-1H- imidazol-2-yl}isonicotinonitrile 1H NMR (CDCl3) δ 9.07 (s, 1 H), 8.68 (d, 1 H), 8.60 (s, 1 H), 8.44 (d, 1 H), 7.45 (d, 1 H), 7.29 (d, 1 H), 7.26 (d, 1 H), 7.22 (d, 1 H), 6.37 (s, 2 H), 4.16 (t, 2 H), 1.63 (q, 2 H), 0.81 (t, 3 H) m/e 344
    869
    Figure US20100267698A1-20101021-C01519
         		2-{[2-(2-methoxyphenyl)-1H- imidazol-1-yl]methyl}-3-propyl- 3H-imidazo[4,5-b]pyridine dihydrochloride 1H NMR (d6 DMSO): 8.33(1H, dd), 8.02- 7.99(2H, m), 7.93(1H, d), 7.65- 7.59(1H, m), 7.51-7.48(1H, m), 7.29 7.21(2H, m), 7.11-7.06(1H, m), 5.75(2H, s), 4.12(2H, t), 3.64(3H, s), 1.69-1.61(2H, m), 0.74(3H, t). 348.3  [M + 1]
    870
    Figure US20100267698A1-20101021-C01520
         		2-{[2-(3-methoxyphenyl)-1H- imidazol-1-yl]methyl}-3-propyl-3H- imidazo[4,5-b]pyridine dihydrochloride 1H NMR (d6 DMSO): 8.35(1H, dd), 8.04- 7.98(2H, m), 7.93(1H, d), 7.48(1H, t), 7.35-7.19(4H, m), 5.95(2H, s), 4.22(2H, t), 3.69(3H, s), 1.79- 1.68(2H, m), 0.81(3H, t) 348.39 [M + 1]
    871
    Figure US20100267698A1-20101021-C01521
         		6-(1-{[3-(2-fluoroethyl)-3H- imidazo[4,5-b]pyridin-2-yl]methyl}- 1H-imidazol-2-yl)pyridine-2- carbonitrile H-1 NMR (CDCl3): line list provided 4.74 (m, 1H), 4.84-4.95 (m, 3H), 6.16 (s, 2H), 7.19-7.28 (m, 2H), 7.39 (s, 1H), 7.54 (d, 1H), 7.83 (t, 1H), 7.89 (d, 1H), 8.34 (m, 1H), 8.53 (d, 1H) 348   [M + 1]
    872
    Figure US20100267698A1-20101021-C01522
         		2-{[2-(3,4-difluorophenyl)-1H- imidazol-1-yl]methyl}-3-(2- fluoroethyl)-3H-imidazo[4,5- b]pyridine H-1 NMR (CDCl3): line list provided 4.33 (t, 1H), 4.42 (t, 1H), 4.56 (t, 1H), 4.73 (t, 1H), 5.51 (s, 2H), 7.07 (s, 1H), 7.18-7.26 (m, 2H), 7.39 (m, 1H), 7.55 (m, 1H), 8.07 (d, 1H), 8.37 (s, 1H) 358   [M + 1]
    873
    Figure US20100267698A1-20101021-C01523
         		3-(2-fluoroethyl)-2-{[2-(2,4,5- trifluorophenyl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine H-1 NMR (CDCl3): line list provided 4.23 (t, 1H), 4.31 (t, 1H), 4.50 (t, 1H), 4.66 (t, 1H), 5.40 (s, 2H), 7.01-7.09 (m, 2H), 7.20-7.26 (m, 2H), 7.43 (m, 1H), 7.99 (d, 1H), 8.32 (d, 1H) 376   [M + 1]
    874
    Figure US20100267698A1-20101021-C01524
         		3-(2-fluoroethyl)-2-{[2-(5-fluoro-2- methylphenyl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine H-1 NMR (CDCl3): line list provided 2.16 (s, 3H), 4.19 (t, 1H), 4.26 (t, 1H), 4.49 (t, 1H), 4.57 (t, 1H), 5.28 (s, 2H), 7.02-7.07 (2H), 7.14 (s, 1H), 7.21-7.26 (m, 3H), 8.02 (d, 1H), 8.35 (d, 1H) 354  
    875
    Figure US20100267698A1-20101021-C01525
         		1-propyl-2-{[2-(1,3-thiazol-2-yl)- 1H-imidazol-1-yl]methyl}-1H- imidazo[4,5-c]pyridine 1H NMR (CDCl3) δ 9.07 (s, 1 H), 8.41 (d, 1 H), 7.82 (d, 1 H), 7.38 (d, 1 H), 7.25 (d, 1 H), 7.19 (s, 1 H), 7.12 (s, 1 H), 6.35 (s, 2 H), 4.14 (t, 2 H), 1.53 (q, 2 H), 0.74 (t, 3 H) m/e 325
    876
    Figure US20100267698A1-20101021-C01526
         		1-ethyl-2-{[2-(1,3-thiazol-2-yl)-1H- imidazol-1-yl]methyl}-1H- imidazo[4,5-c]pyridine 1H NMR (CDCl3) δ 9.08 (s, 1 H), 8.43 (d, 1 H), 7.85 (d, 1 H), 7.38 (d, 1 H), 7.38-7.26 (m, 1 H), 7.17 (s, 1 H), 7.14 (s, 1 H), 6.35 (s, 2 H), 4.25 (q, 2 H), 1.11 (t, 3 H) m/e 311
    877
    Figure US20100267698A1-20101021-C01527
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-6-methyl-1- propyl-1H-imidazo[4,5-c]pyridine 1H NMR (CDCl3) δ 8.93 (s, 1 H), 8.16 (d, 1 H), 7.87 (dd, 1 H), 7.20 (d, 1 H), 7.16 (d, 1 H), 7.10 (d, 1 H), 6.87 (d, 1 H), 6.26 (s, 2 H), 4.19 (t, 2 H), 2.65 (s, 3 H), 1.65 (q, 2 H), 0.83 (t, 3 H) m/e 351
    878
    Figure US20100267698A1-20101021-C01528
         		2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-6-methyl-1- propyl-1H-imidazo[4,5-c]pyridine 1H NMR (CDCl3) δ 8.95 (s, 1 H), 7.49-7.36 (m, 3 H), 7.19-7.15 (m, 2 H), 7.04 (m, 2 H), 5.48 (s, 2 H), 3.64 (t, 2 H), 2.64 (s, 3 H), 1.40 (q, 2 H), 0.73 (t, 3 H)
    879
    Figure US20100267698A1-20101021-C01529
         		3-(cyclopropylmethyl)-2-{[2-(1,3- thiazol-2-yl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine dihydrochloride 1H NMR (d6 DMSO): 8.35(1H, dd), 7.95(1H, dd), 7.90-7.87(2H, m), 7.81(1H, s), 7.52(1H, s), 7.27(1H, dd), 6.35(2H, s), 4.31(2H, d), 1.42-1.38(1H, m), 0.55-0.52(4H, m) 335.1  [M − 1]
    880
    Figure US20100267698A1-20101021-C01530
         		2-{1-[(3-propyl-3H-imidazo[4,5- b]pyridin-2-yl)methyl]-1H- imidazol-2-yl}benzonitrile dihydrochloride 1H NMR (d6 DMSO): 8.31(1H, d), 8.15(1H, s), 8.05-8.03(2H, m), 7.94-7.92(2H, m), 7.85-7.77(2H, m), 7.27-7.24(1H, m), 5.97(1H, s), 4.14(2H, t), 1.72- 1.64(2H, m), 0.75(3H, t) 343.3  [M + 1]
    881
    Figure US20100267698A1-20101021-C01531
         		2-{[2-(5-methylisoxazol-3-yl)-1H- imidazol-1-yl]methyl}-3-propyl-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.39(dd, 1H), 8.04(dd, 1H), 7.19-7.27(m, 3H), 6.65(d, 1H), 6.10(s, 2H), 4.26(t, 2H), 1.63(m, 2H), 0.83(t, 3H) 323.2 
    882
    Figure US20100267698A1-20101021-C01532
         		3-isopropyl-2-{[2-(1,3-thiazol-2- yl)-1H-imidazol-1-yl]methyl}-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.37(dd, 1H), .8.02(dd, 1H), 7.84 (d, 1H), 7.39(d, 1H), 7.11-7.23(m, 3H), 6.32(s, 2H), 4.92(m, 1H), 1.56(d, 6H) 325.4 
    883
    Figure US20100267698A1-20101021-C01533
         		1-(1-ethyl-2-{[2-(1,3-thiazol-2-yl)- 1H-imidazol-1-yl]methyl}-1H- benzimidazol-5-yl)ethanone 1H NMR, δ ppm (CDCl3): 8.39(d, 1H), .8.00(dd, 1H) 7.84 (d, 1H), 7.39(d, 1H), 7.37(d, 1H), 7.18(d, 1H), 7.13(d, 1H), 6.35(s, 2H), 4.27(q, 2H), 2.67(s, 3H), 1.12(t, 3H) 352.2 
    884
    Figure US20100267698A1-20101021-C01534
         		2-{[2-(2,3-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl-1H- benzimidazole-5-carbonitrile 1H NMR, δ ppm (CDCl3): 8.09(dd, 1H), 7.55 (dd, 1H), 7.19-7.40(m, 5H), 7.04(d, 1H), 5.40(s, 2H), 3.86(q, 2H), 1.02(t, 3H)
    885
    Figure US20100267698A1-20101021-C01535
         		2-{[2-(5-fluoro-2-methylphenyl)- 1H-imidazol-1-yl]methyl}-3-propyl- 3H-imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.39(dd, 1H), 8.03(dd, 1H), 7.20-7.31(m, 3H), 7.07-7.13(m, 3H), 5.24(s, 2H), 3.88(t, 2H), 2.22(s, 3H), 1.54(m, 2H), 0.78(t, 3H) 350.3 
    886
    Figure US20100267698A1-20101021-C01536
         		2-{[2-(2-fluoro-5-methylphenyl)- 1H-imidazol-1-yl]methyl}-3-propyl- 3H-imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.38(dd, 1H), 8.04(dd, 1H), 7.44(dd, 1H), 7.22-7.30(m, 2H), 7.19(d, 1H), 7.12(dd, 1H), 7.05(d, 1H), 5.37(s, 2H), 3.84(t, 2H), 2.36 (s, 3H), 1.46(m, 2H), 0.76(t, 3H) 350.3 
    887
    Figure US20100267698A1-20101021-C01537
         		2-{[2-(5-fluoro-2-methoxyphenyl)- 1H-imidazol-1-yl]methyl}-3-propyl- 3H-imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.39(dd, 1H), 8.03(dd, 1H), 7.15-7.32(m, 4H), 7.03(d, 1H), 6.96(dd, 1H), 5.30(s, 2H), 3.84(t, 2H), 3.79(s, 3H), 1.46(m, 2H), 0.76(t, 3H) 366.2 
    888
    Figure US20100267698A1-20101021-C01538
         		1-ethyl-2-({2-[3- (trifluoromethyl)phenyl]-1H- imidazol-1-yl}methyl)-1H- imidazo[4,5-c]pyridine dihydrochloride 1H NMR (d6 DMSO): 10.02(1H, s), 8.70(1H, d), 8.37(1H, d), 8.08(1H, s), 7.97- 7.94(3H, m), 7.82(1H, s), 7.75(1H, t), 6.09(2H, s), 4.45(2H, q), 1.32(3H, t).
    889
    Figure US20100267698A1-20101021-C01539
         		1-ethyl-2-{[2-(5-fluoro-2- methylphenyl)-1H-imidazol-1- yl]methyl}-1H-benzimidazole-5- carbonitrile hydrochloride 1H NMR (d6 DMSO): 8.10(1H, s), 8.07(1H, d), 7.95(1H, d), 7.78(1H, d), 7.64(1H, dd), 7.52(1H, dd), 7.38-7.35(2H, m), 5.82(2H, s), 4.23(2H, q), 2.17(3H, s), 1.18(3H, t).
    890
    Figure US20100267698A1-20101021-C01540
         		2-{[2-(2,6-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-propyl-1H- imidazo[4,5-c]pyridine 1H NMR (CDCl3) δ 9.05 (s, 1H), 8.43 (d, 1 H), 7.5-7.4 (m, 1 H), 7.27-7.23 (m, 2 H), 7.07-7.01 (m, 3 H), 5.34 (s, 2 H), 3.75 (t, 2H), 1.47 (q, 2 H), 0.77 (t, 3 H) m/e 354
    891
    Figure US20100267698A1-20101021-C01541
         		2-{[2-(2,6-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl-1H- imidazo[4,5-c]pyridine 1H NMR (CDCl3) δ 9.05 (s, 1 H), 8.43 (d, 1 H), 7.52-7.42 (m, 1 H), 7.27-7.23 (m, 2 H), 7.08-7.01 (m, 3 H), 5.35 (s, 2 H), 3.85 (q, 2 H), 1.02 (t, 3 H) m/e 340
    892
    Figure US20100267698A1-20101021-C01542
         		2-{[2-(3,4-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl-1H- imidazo[4,5-c]pyridine 1H NMR (CDCl3) δ 9.10 (s, 1 H), 8.46 (d, 1 H), 7.56-7.51 (m, 1 H), 7.42-7.30 (m, 1 H), 7.31-7.26 (m, 2 H), 7.16 (d, 1 H), 7.03 (d, 1 H), 5.48 (s, 2 H), 3.83 (q, 2 H), 1.05 (t, 3 H) m/e 340
    893
    Figure US20100267698A1-20101021-C01543
         		2-{[2-(5-fluoro-2-methylphenyl)- 1H-imidazol-1-yl]methyl}-1-propyl- 1H-imidazo[4,5-c]pyridine 1H NMR (CDCl3) δ 9.07 (s, 1 H), 8.43 (d, 1 H), 7.31-7.23 (m, 3 H), 7.12-7.04 (m, 3 H), 5.24 (s, 2 H), 3.73 (t, 2 H), 2.21 (s, 3 H), 1.49 (q, 2 H), 0.75 (t, 3 H) m/e 350
    894
    Figure US20100267698A1-20101021-C01544
         		1-ethyl-2-{[2-(5-fluoro-2- methylphenyl)-1H-imidazol-1- yl]methyl}-1H-imidazo[4,5- c]pyridine 1H NMR (CDCl3) δ 9.08 (s, 1 H), 8.44 (d, 1 H), 7.31-7.24 (m, 3 H), 7.12-7.04 (m, 3 H), 5.25 (s, 1 H), 3.83 (q, 2 H), 2.22 (s, 3 H), 1.06 (t, 3 H) m/e 336
    895
    Figure US20100267698A1-20101021-C01545
         		2-{[2-(1,3-thiazol-2-yl)-1H- imidazol-1-yl]methyl}-3-(2,2,2- trifluoroethyl}-3H-imidazo[4,5- b]pyridine H-1 NMR (CDCl3): line list provided 5.32 (q, 2H), 6.19 (s, 2H), 7.19 (s, 1H), 7.26 (m, 2H), 7.43 (s, 1H), 7.79 (d, 1H), 8.04 (d, 1H), 8.41 (m, 1H) 365   [M + 1]
    896
    Figure US20100267698A1-20101021-C01546
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-3-(2,2,2- trifluoroethyl)-3H-imidazo[4,5- b]pyridine HCl salt H-1 NMR (CD3OD): 5.42 (q, 2H), 6.40 (s, 2H), 77.24 (d, 1H), 7.37 (m, 1H), 7.90 (m, 2H), 8.03 (m, 2H), 8.22 (m, 1H), 8.43 (m, 1H) 377   [M + 1]
    897
    Figure US20100267698A1-20101021-C01547
         		2-{[2-(3,4-difluorophenyl)-1H- imidazol-1-yl]methyl}-3-ethyl-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.39(dd, 1H), 8.04(dd, 1H), 7.54(m, 1H), 7.40(m, 1H), 7.26-7.32(m, 2H), 7.16(d, 1H), 7.04(d, 1H), 5.47(s, 2H), 4.02(q, 2H), 1.09(t, 3H) 340.2 
    898
    Figure US20100267698A1-20101021-C01548
         		2-{[2-(1-methyl-1H-pyrazol-3-yl)- 1H-imidazol-1-yl]methyl}-3-propyl- 3H-imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.38(1H, dd), 8.05(1H, dd), 7.42(1H, d), 7.23(1H, dd), 7.09(1H, d), 7.04(1H, d), 6.91(1H, d), 6.21(2H, s), 4.19(2H, t), 3.97(3H, s), 1.61-1.50(2H, m), 0.76(3H, t) 322.3  [M + 1]
    899
    Figure US20100267698A1-20101021-C01549
         		2-{[2-(2,3-difluorophenyl)-1H- imidazol-1-yl]methyl}-1-ethyl-1H- imidazo[4,5-c]pyridine dihydrochloride 1H NMR (d6 DMSO): 9.44(1H, s), 8.70(1H, d), 8.37(1H, d), 8.04(1H, s), 7.89(1H, s), 7.73(1H, q), 7.49(1H, d), 7.37(1H, t), 6.05(2H, s), 4.44(2H, q), 1.30(3H, t) 340.2 
    900
    Figure US20100267698A1-20101021-C01550
         		1-propyl-2-[(2-quinolin-2-yl-1H- imidazol-1-yl)methyl]-1H- benzimidazole-5-carbonitrile dihydrochloride 1H NMR (d6 DMSO): 8.60(1H, d), 8.3591H, d), 8.02-7.99(2H, m), 7.88-7.85(2H, m), 7.79-7.60(5H, m), 6.54(2H, s), 4.47(2H, t), 1.85-1.77(2H, m), 0.89(3H, t) 393.4  [M + 1]
    901
    Figure US20100267698A1-20101021-C01551
         		2-{[2-(2,4-difluorophenyl)-1H- imidazol-1-yl]methyl}-3-ethyl-3H- imidazo[4,5-b]pyridine dihydrochloride 1H NMR (d6 DMSO): 8.35-8.33(1H, m), 8.07- 8.06(1H, m), 7.99-7.96(2H, m), 7.86- 7.80(1H, m), 7.63-7.57(1H, m), 7.35- 7.32(1H, m), 7.28-7.25(1H, m), 5.89(2H, s), 4.27(2H, q), 1.28(3H, t) 340.2  [M + 1]
    902
    Figure US20100267698A1-20101021-C01552
         		1-Propyl-2-(2-pyrimidin-2-yl- imidazol-1-ylmethyl}-1H- benzoimidazole-5-carbonitrile dihydrochloride 1H NMR (d6 DMSO): 8.92(2H, d), 8.18(1H, d), 8.00-7.99(2H, m), 7.88(1H, dd), 7.65-7.62(2H, m), 6.46(2H, s), 4.42(2H, t), 1.93-1.84(2H, m), 0.97(3H, t) 344.2  [M + 1]
    903
    Figure US20100267698A1-20101021-C01553
         		3-ethyl-2-{[2-(2,3,6- trifluorophenyl)-1H-imidazol-1- yl]methyl}-3H-imidazo[4,5- b]pyridine 1H NMR, δ ppm (CDCl3): 8.40(dd, 1H), 8.02(dd, 1H), 7.24-7.32(m, 3H), 7.11(d, 1H), 7.00(m, 1H), 5.36(s, 2H), 4.02(q, 2H), 1.09(t, 3H) 358.2 
    904
    Figure US20100267698A1-20101021-C01554
         		3-propyl-2-[(2-thien-3-yl-1H- imidazol-1-yl)methyl]-3H- imidazo[4,5-b]pyridine 1H NMR, δ ppm (CDCl3): 8.40(dd, 1H), 8.05(dd, 1H), 7.66(dd, 1H), 7.43-7.49(m, 2H), 7.25(dd, 1H), 7.14(d, 1H), 7.02(d, 1H), 5.55(s, 2H), 3.91(t, 2H), 1.51(m, 2H), 0.79(t, 3H) 324.3 
    905
    Figure US20100267698A1-20101021-C01555
         		1-{1-ethyl-2-[(2-pyrimidin-2-yl- 1H-imidazol-1-yl)methyl]-1H- benzimidazol-5-yl}ethanone 1H NMR, δ ppm (CDCl3): 8.87(d, 2H), .8.41(dd, 1H), 8.02(dd, 1H), 7.40(dd, 1H), 7.26-7.31(m, 2H), 7.16(d, 1H), 6.40(s, 2H), 4.27(q, 2H), 2.69(s, 3H), 1.17(t, 3H)
    906
    Figure US20100267698A1-20101021-C01556
         		1-ethyl-2-{[2-(6-fluoropyridin-2-yl)- 1H-imidazol-1-yl]methyl}-6- methyl-1H-imidazo[4,5-c]pyridine hu 1H NMR (CDCl3) δ 8.93 (s, 1 H), 8.16 (d, 1 H), 7.87 (dd, 1 H), 7.18 (d, 1 H), 7.12 (s, 1 H), 6.87 (d, 1 H), 6.26 (s, 2 H), 4.30 (q, 2 H), 2.65 (s, 3 H), 1.22 (t, 3 H) m/e 337
    907
    Figure US20100267698A1-20101021-C01557
         		1-ethyl-2-{[2-(3-fluorophenyl)-1H- imidazol-1-yl]methyl}-6-methyl- 1H-imidazo[4,5-c]pyridine dihydrochloride 1H NMR (DMSO- d6) δ 9.28 (s, 1 H), 8.24 (s, 1 H), 8.23 (d, 1 H), 7.94 (d, 1 H), 7.69- 7.48 (m, 4 H), 6.12 (s, 2 H), 4.39 (q, 2 H), 2.77 (s, 3 H), 1.35 (t, 3 H); m/e 336
    908
    Figure US20100267698A1-20101021-C01558
         		3-ethyl-2-({2-[2-fluoro-5- (trifluoromethyl)phenyl]-1H- imidazol-1-yl}methyl)-3H- imidazo[4,5-b]pyridine H-1 NMR (CDCl3): line list provided 1.08 (t, 3H), 3.99 (q, 2H), 5.38 9s, 2H), 7.10 (s, 1H), 7.22-7.39 (m, 2H), 7.75 (m, 1H), 7.93 (m, 1H), 8.02 (d, 1H), 8.38 (d, 1H) 390   (M + 1)
    909
    Figure US20100267698A1-20101021-C01559
         		2-{[2-(2-methylphenyl)-1H- imidazol-1-yl]methyl}-3-propyl- 3H-imidazo[4,5-b]pyridine H-1 NMR (CDCl3): line list provided 0.73 (t, 3H), 1.49 (m, 2H), 2.24 (s, 3H), 3.81 (m, 2H), 5.22 (s, 2H), 7.10 (s, 1H), 7.18-7.36 (m, 6H), 8.02 (d, 1H), 8.37 (d, 1H) 332   [M + 1]
    910
    Figure US20100267698A1-20101021-C01560
         		6-{1-[(3-cyclopropyl-3H- imidazo[4,5-b]pyridin-2-yl)methyl]- 1H-imidazol-2-yl}pyridine-2- carbonitrile H-1 NMR (CDCl3): line list provided 1.28 (m, 4H), 3.37 (m, 1H), 3.48 (s, 2H), 6.36 (s, 2H), 7.15- 7.28 (m, 3H), 7.56 (d, 1H), 7.86 (m, 2H), 8.38 (m, 1H), 8.54 (d, 1H) 342   [M + 1]; 340   (M − 1)
    911
    Figure US20100267698A1-20101021-C01561
         		4-fluoro-3-{1-[(3-propyl-3H- imidazo[4,5-b]pyridin-2-yl)methyl]- 1H-imidazol-2-yl}benzonitrile CDCl3: 8.38 (dd, J = 1.48, 4.81 Hz, 1H), 7.98 (dt, J = 1.48, 7.35 Hz, 2H), 7.72-7.78 (m, 1H), 7.35 (t, J = 9.07 Hz, 1H), 7.22-7.26 (m, 2H), 7.11 (d, J = 1.1 Hz, 1H), 5.37 (s, 2H), 3.93 (t, J = 7.69 Hz, 2H), 1.54 (dq, J = 7.42, 7.69 Hz, 2H), 361.2  [M + 1]
    912
    Figure US20100267698A1-20101021-C01562
         		1-propyl-2-({2-[3- (trifluoromethyl)phenyl]-1H- imidazol-1-yl}methyl)-1H- imidazo[4,5-c]pyridine 1H NMR (CDCl3): 9.09(1H, s), 8.43(1H, d), 7.93(1H, s), 7.84(1H, d), 7.72(1H, d), 7.62(1H, t), 7.25(1H, ), 7.19(1H, d), 7.09(1H, d), 5.50(2H, s), 3.63(2H, t), 1.49- 1.39(2H, m), 0.75(3H, t).
    913
    Figure US20100267698A1-20101021-C01563
         		2-{[2-(6-fluoropyridin-2-yl)-1H- imidazol-1-yl]methyl}-1-propyl- 1H-benzimidazole-5- carbohydrazide CD3OD: 8.10-8.29 (m, 6H), 8.03 (S, 1H), 7.29 (d, 1H), 6.4 (s, 2H), 4.59-4.65 (m, 2H), 2.0-2.12 (m, 2H), 1.06-1.07 (m, 3H). 394.3  [M + 1]
    Figure US20100267698A1-20101021-P00899
    indicates data missing or illegible when filed
  • TABLE 7
    Cmp. MS
    # STRUCTURE IUPAC NAME NMR (m/z)
    914
    Figure US20100267698A1-20101021-C01564
         		1-ethyl-2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}- 5-(morpholin- 4-ylmethyl)-1H-benzimidazole 420.3 [M + 1]
    915
    Figure US20100267698A1-20101021-C01565
         		ethyl 1-ethyl-2-{[1-(3- fluorophenyl)-1H-pyrazol-5- yl]methyl}-1H- benzimidazole-5- carboxylate 393.3 [M + 1]
    916
    Figure US20100267698A1-20101021-C01566
         		1-ethyl-2-{[1- (3-fluorophenyl)-1H- pyrazol-5-yl]methyl}-1H- benzimidazole-5- carboxylic acid 365.2 [M + 1]
    917
    Figure US20100267698A1-20101021-C01567
         		N-[2-(dipropylamino)ethyl]- 1-ethyl- 2-{[1-(3-fluorophenyl)- 1H-pyrazol- 5-yl]methyl}- 1H-benzimidazole-5- carboxamide 491.4 [M + 1]
    918
    Figure US20100267698A1-20101021-C01568
         		N,1-diethyl-2-{[1- (3-fluorophenyl)- 1H-pyrazol-5-yl]methyl}-1H- benzimidazole-5-carboxamide 392.3 [M + 1]
    919
    Figure US20100267698A1-20101021-C01569
         		1-ethyl-2-{[1- (3-fluorophenyl)-1H- pyrazol-5-yl]methyl}- N-phenyl-1H- benzimidazole-5-carboxamide 392.3 [M + 1]
    920
    Figure US20100267698A1-20101021-C01570
         		1-ethyl-2-{[1- (3-fluorophenyl)-1H- pyrazol-5-yl]methyl}- 5-[(4- methylpiperazin-1-yl)methyl]-1H- benzimidazole 1H NMR (CDCl3): 7.65 (d, 1H), 7.61 (d, 1H), 7.45 (m, 1H), 7.22-7.31 (m, 4H), 7.11 (m, 1H), 6.16 (d, 1H), 4.31 (s, 2H), 3.96 (q, 2H), 3.62 (s, 2H), 2.40-2.60 (m, 8H), 2.25 (s, 3H), 1.22 (t, 3H)
    921
    Figure US20100267698A1-20101021-C01571
         		1-ethyl-5- [(4-ethylpiperazin-1- yl)methyl]-2-{[1-(3- fluorophenyl)- 1H-pyrazol-5-yl]methyl}-1H- benzimidazole 1H NMR (CDCl3): 7.65 (d, 1H), 7.62 (d, 1H), 7.45 (m, 1H), 7.23-7.30 (m, 4H), 7.13 (m, 1H), 6.16 (d, 1H), 4.31 (s, 2H), 3.96 (q, 2H), 3.62 (s, 2H), 2.40-2.60 (m, 10H), 1.21 (t, 3H), 1.078 (t, 3H)
    922
    Figure US20100267698A1-20101021-C01572
         		5-[(4-cyclopentylpiperazin-1- yl)methyl]-1-ethyl-2-{[1-(3- fluorophenyl)-1H-pyrazol-5- yl]methyl}-1H-benzimidazole 1H NMR (CDCl3): 7.65 (d, 1H), 7.61 (d, 1H), 7.45 (m, 1H), 7.22-7.31 (m, 4H), 7.13 (m, 1H), 6.15 (d, 1H), 4.31 (s, 2H), 3.96 (q, 2H), 3.63 (s, 2H), 2.40-2.60 (m, 9H), 1.31-1.90 (m, 8H), 1.21 (t, 3H.)
    923
    Figure US20100267698A1-20101021-C01573
         		5-[(4-cycloheptylpiperazin-1- yl)methyl]-1-ethyl-2-{[1-(3- fluorophenyl)-1H-pyrazol-5- yl]methyl}-1H-benzimidazole 515.5 [M + 1]
    924
    Figure US20100267698A1-20101021-C01574
         		2-{[1-(3-chlorophenyl)- 1H-pyrazol- 5-yl]methyl}-1- ethyl-5-(morpholin- 4-ylmethyl)-1H- benzimidazole 436.3 [M + 1]; 434.3 [M − 1]
    925
    Figure US20100267698A1-20101021-C01575
         		1′-[(1-ethyl-2-{[1- (3-fluorophenyl)- 1H-pyrazol-5-yl]methyl}-1H- benzimidazol-5-yl) methyl]-1,4′- bipiperidine 1H NMR (CDCl3): 7.63 (d, 1H), 7.61 (d, 1H), 7.44 (m, 1H), 7.2307.31 (m, 4H), 7.13 (m, 1H), 6.16 (d, 1H), 4.31 (s, 2H), 3.96 (q, 2H), 3.61 (s, 2H), 2.96 (m, 2H), 2.50 (m, 4H), 1.40-2.31 (m, 13H), 1.23 (t, 3H)
    926
    Figure US20100267698A1-20101021-C01576
         		1-ethyl-2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}-5-[(4- pyrrolidin-1-ylpiperidin-1- yl)methyl]-1H-benzimidazole
    927
    Figure US20100267698A1-20101021-C01577
         		1-ethyl-2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}-5-[(4- phenylpiperazin-1-yl) methyl]-1- benzimidazole 1H NMR (CDCl3): 7.69 (d, 1H), 7.62 (s, 1H), 7.45 (m, 1H), 7.21-7.33 (m, 6H), 7.13 (m, 1H), 6.82-6.94 (m, 3H), 6.16 (d, 1H), 4.33 (s, 2H), 3.97 (q, 2H), 3.69 (s, 2H), 3.15-3.23 (m, 4H), 2.69-2.67 (m, 4H), 1.21 (t, 3H)
    928
    Figure US20100267698A1-20101021-C01578
         		2-{[1-(3- chlorophenyl)-1H-pyrazol- 5-yl]methyl}-5-[(4- cycloheptylpiperazin-1- yl)carbonyl]-1-ethyl-1H- benzimidazole 545.4 [M + 1]; 543.4 [M − 1]
    929
    Figure US20100267698A1-20101021-C01579
         		1-ethyl-2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}-1H- benzimidazole- 5-carboxamide
    930
    Figure US20100267698A1-20101021-C01580
         		1-ethyl-5-fluoro-2-{[1-(3- fluorophenyl)-1H-pyrazol-5- yl]methyl}-1H-benzimidazole (L)-Tartrate salt 1H NMR (CD3OD): 7.67 (s, 1H), 7.05-7.46 (m, 7H), 6.31 (s, 1 H), 4.53 (s, 2H), 4.47 (s, 2H), 4.14 (q, 2H), 1.21 (t, 3H)
    931
    Figure US20100267698A1-20101021-C01581
         		5-chloro-1-ethyl-2-{[1-(3- fluorophenyl)-1H-pyrazol-5- yl]methyl}-1H-benzimidazole (L)-Tartrate salt 1H NMR (d6- DMSO): 7.65 (s, 1H), 7.21-7.58 (m, 7H), 6.26 (s, 1H), 4.48 (s, 2H), 4.30 (s, 2H), 4.16 (q, 2H), 1.15 (t, 3H)
    932
    Figure US20100267698A1-20101021-C01582
         		5-bromo-1-ethyl-2-{[1-(3- fluorophenyl)-1H-pyrazol-5- yl]methyl)-1H-benzimidazole (L)-Tartrate salt 1H NMR (d6- DMSO): 7.72 (d, 1H), 7.65 (d, 1H), 7.21-7.53 (m, 6H), 6.26 (s, 1H), 4.48 (s, 2H), 4.30 (s, 2H), 4.16 (q, 2H), 1.14 (t, 3H)
    933
    Figure US20100267698A1-20101021-C01583
         		1-ethyl-2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}- 5-phenyl-1H- benzimidazole 1H NMR (CDCl3): 7.96 (d, 1H) 7.27-7.66 (m, 11H), 7.14 (m, 1H), 6.21 (d, 1H), 4.38 (s, 2H), 4.01 (q, 2H), 1.25 (t, 3H)
    934
    Figure US20100267698A1-20101021-C01584
         		2-{[1-(2,5-difluorophenyl)-1H- pyrazol-5-yl]methyl}- 1-ethyl-1H- benzimidazole-5-carbonitrile 364.2 [M + 1]
    935
    Figure US20100267698A1-20101021-C01585
         		2-{[1-(3- chlorophenyl)-1H-pyrazol- 5-yl]methyl}-1-ethyl-1H- benzimidazole-5-carbonitrile 1H NMR (CDCl3): 8.05 (d, 1H), 7.36-7.65 (m, 7H), 6.21 (d, 1H), 4.36 (s, 2H), 4.02 (q, 2H), 1.24 (t, 3H)
    936
    Figure US20100267698A1-20101021-C01586
         		2-{[1-(2,5-difluorophenyl)-1H- pyrazol-5-yl]methyl}- 1-ethyl-1H- benzimidazole-5-amine 354.2 [M + 1]
    937
    Figure US20100267698A1-20101021-C01587
         		2-{[1-(2,5-difluorophenyl)-1H- pyrazol-5-yl]methyl}- 1-ethyl-5-(4H 1,2,4-triazol-4-yl)-1H- benzimidiazole 405.95 [M + 1]
    938
    Figure US20100267698A1-20101021-C01588
         		1-ethyl-2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}-5- (1H-1,2,4- triazol-1-ylmethyl)-1H- benzimidiazole 402.4 [M + 1]
    939
    Figure US20100267698A1-20101021-C01589
         		1-ethyl-2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}-5- (1H-tetraazol-1-ylmethyl)-1H- benzimidiazole 403.2 [M + 1]; 401.4 [M − 1]
    940
    Figure US20100267698A1-20101021-C01590
         		1-ethyl-2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}-5-(2H- tetraazol-2-ylmethyl)-1H- benzimidiazole 403.4 [M + 1]; 401.4 [M − 1]
    941
    Figure US20100267698A1-20101021-C01591
         		1-ethyl-2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}-5- [(methylthio)methyl]-1H- benzimidazole 381.3 [M + 1]; 379.3 [M − 1]
    942
    Figure US20100267698A1-20101021-C01592
         		2-{[1-(2,5- difluorophenyl)-1H- pyrazol-5-yl]methyl}- 1-ethyl-5- nitro-1H-benzimidiazole Mesylate 1H NMR (d6 DMSO) 8.38 (1H, d), 8.15-8.12 (1H, m), 7.77 (lH, d), 7.71 (1H, s), 7.51-7.34 (3H, m), 6.40 (lH, s), 4.21 (2H, q), 2.32 (3H, s), 1.11 (3H, t). 384.2 [M + 1]
    943
    Figure US20100267698A1-20101021-C01593
         		1-ethyl-2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}- 5-(1,3-thiazol 5-yl)-1H-benzimidazole Hydrochloride 1H NMR (CD3OD): 9.725 (s, 1H), 8.67 (s, 1H), 8.13 (s, 1H), 8.01-8.08 (m, 2H), 7.79 (s, 1H), 7.21-7.57 (m, 4H), 6.59 (d, 1H), 4.97 (s, 2H), 4.50 (q, 2H), 1.39 (t, 3H)
    944
    Figure US20100267698A1-20101021-C01594
         		1-ethyl-2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}- N-methoxy- N-methyl-1H- benzimidazole-5- carboxamide 1H NMR (CDCl3): 8.13 (d, 1H), 7.63-7.70 (m, 2H), 7.24-7.49 (m, 4H), 7.13 (m, 1H), 6.19 (d, 1H), 4.36 (s, 2H), 4.00 (q, 2H), 3.60 (s, 3H), 3.40 (s, 3H), 1.23 (t, 3H)
    945
    Figure US20100267698A1-20101021-C01595
         		3-(1-ethyl-2-{[1- (3-fluorophenyl)- 1H-pyrazol-5-yl] methyl}-1H- benzimidazol- 5-yl)benzonitrile 1H NMR (CDCl3): 7.84-7.92 (m, 3H), 7.40-7.64 (m, 6H), 7.26-7.33 (m, 2H), 7.14 (m, 1H), 6.21 (d, 1H), 4.37 (s, 2H), 4.03 (q, 2H), 1.26 (t, 3H)
    946
    Figure US20100267698A1-20101021-C01596
         		N-[3-(1-ethyl-2-{[1-(3- fluorophenyl)-1H-pyrazol-5- yl]methyl}- 1H-benzimidazol-5- yl)phenyl]acetamide 1H NMR (CDCl3): 7.93 (d, 1H), 7.26-7.72 (m, 10H), 7.14 (m, 1H), 6.20 (d, 1H), 4.36 (s, 2H), 4.01 (q, 2H), 2.21 (s, 3H), 1.24 (t, 3H)
    947
    Figure US20100267698A1-20101021-C01597
         		1-ethyl-2-{[1- (3-fluorophenyl)-1H- pyrazol-5-yl]methyl}-5-[4- (trifluoromethoxy) phenyl]-1H- benzimidazole 1H NMR (CDCl3): 7.92 (d, 1H), 7.27-7.65 (m, 10H), 7.14 (m, 1H), 6.20 (d, 1H), 4.37 (s, 2H), 4.08 (q, 2H), 1.25 (t, 3H)
    948
    Figure US20100267698A1-20101021-C01598
         		5-(3-chloro-4- fluorophenyl)-1- ethyl-2-{[1- (3-fluorophenyl)-1H- pyrazol-5-yl]methyl}-1H- benzimidazole 1H NMR (CDCl3): 7.897 (d, 1H) 7.63-7.66 (m, 2H), 7.11-7.50 (m, 8H), 6.21 (d, 1H), 4.36 (s, 2H), 4.02 (q, 2H), 1.25 (t, 3H)
    949
    Figure US20100267698A1-20101021-C01599
         		1-ethyl-5-(3-fluorophenyl)- 2-{[1- (3-fluorophenyl)- 1H-pyrazol-5- yl]methyl}- 1H-benzimidazole 1H NMR (CDCl3): 7.95 (d, 1H), 7.64 (d, 1H), 7.27-7.53 (m, 8H)/ 7.14 (m, 2H), 7.03 (m, 1H), 6.20 (d, 1H), 4.36 (s, 2H), 4.02 (q, 2H), 1.25 (t, 3H)
    950
    Figure US20100267698A1-20101021-C01600
         		1-ethyl-2-{[1- (3-fluorophenyl)-1H- pyrazol-5-yl] methyl}-5-pyridin-3-yl- 1H-benzimidazole 1H NMR (CDCl3): 8.90 (d, 1H), 8.59 (m, 1H), 7.90-7.95 (m, 2H), 7.64 (d, 1H), 7.25-7.53 (m, 6H), 7.14 (m, 1H), 6.21 (d, 1H), 4.37 (s, 2H), 4.03 (q 2H), 1.26 (t, 3H)
    951
    Figure US20100267698A1-20101021-C01601
         		1-ethyl-2-{[1- (3-fluorophenyl)-1H- pyrazol-5-yl] methyl}-5-pyridin-4-yl- 1H-benzimidazole 1H NMR (CDCl3): 8.67 (d, 1H), 8.65 (d, 1H), 8.03 (d, 1H), 7.26-7.65 (m, 8H), 7.15 (m, 1H), 6.21 (d, 1H), 4.37 (s, 2H), 4.03 (q, 2H), 1.26 (t, 3H)
    952
    Figure US20100267698A1-20101021-C01602
         		2-{[1-(2,5- difluorophenyl)-1H- pyrazol-5-yl] methyl}-1-ethyl-5- (methylidyne- lamda~5~-azanyl)- 1H-benzimidazole 1H NMR (CDCl3): 7.70-7.67 (2H, m), 7.31-7.14 (5H, m), 6.16 (1H, s), 4.28 (2H, s), 4.02 (2H, q), 1.22 (3H, t) 364.1 [M + 1]
    953
    Figure US20100267698A1-20101021-C01603
         		N-(2-{[1-(2,5- difluorophenyl)-1H- pyrazol-5-yl]methyl}- 1-ethyl-1H- benzimidazol-5-yl)-2,2,2- trifluoroacetamide 449.96 [M + 1]
    954
    Figure US20100267698A1-20101021-C01604
         		2-{[1-(2,5- difluorophenyl)-1H- pyrazol-5-yl] methyl}-1-ethyl-5-(1H tetraazal-1-yl)- 1H-benzimidazole 1H NMR (DMSO): 10.07 (s, 1H0, 8.07 (s, 1H), 7.86 (d, 1H), 7.79 (d, 1H), 7.71 (s, 1H), 7.36-7.57 (m, 3H), 6.4 (s, 2H), 4.46 (s, 2H), 4.21 (q, 2H0, 1.14 (t, 3H)
    955
    Figure US20100267698A1-20101021-C01605
         		1-(1-ethyl-2-{[1- (3-fluorophenyl)- 1H-pyrazol-5-yl]methyl}-1H- benzimidazol-5-yl)ethanol
    956
    Figure US20100267698A1-20101021-C01606
         		2-{[1-(3- chlorophenyl)-1H-pyrazol- 5-yl]methyl}- 1-ethyl-N-methoxy-N- methyl-1H-benzimidazole-5- carboxamide 1H NMR (CDCl3): 8.13 (d, 1H), 7.63-7.70 (m, 2H), 7.51 (m, 1H), 7.31-7.41 (m, 4H), 6.20 (d, 1H), 4.35 (s, 2H), 4.00 (q, 2H), 3.60 (s, 3H), 3.40 (s, 3H), 1.23 (t, 3H)
    957
    Figure US20100267698A1-20101021-C01607
         		2-{[1- (2,5-difluorophenyl)-1H- pyrazol-5-yl]methyl}- 1-ethyl-5-(5- methyl-1,3,4- oxadiazol-2-yl)-1H- benzimidazole 437.02 [M + 1]
    958
    Figure US20100267698A1-20101021-C01608
         		1-(2-{[1-(3-chlorophenyl)-1H- pyrazol-5-yl] methyl}-1-ethyl-1H- benzimidazol-5-yl)ethanone 1H NMR (CDCl3): 8.37 (d, 1H), 8.03 (dd, 1H), 7.65 (d, 1H), 7.38-7.50 (m, 5H), 6.24 (d, 1H), 4.49 (s, 2H), 4.03 (q, 2H), 2.68 (s, 3H), 1.24 (t, 3H)
    959
    Figure US20100267698A1-20101021-C01609
         		2-{[1- (2,5-difluorophenyl)-1H- pyrazol-5-yl]methyl}- 1-ethyl-5-[5- (trifluoromethyl)- 1H-tetraazol-1- yl]-1H-benzimidazole 474.99 [M + 1]
    960
    Figure US20100267698A1-20101021-C01610
         		5-(3,5- difluorophenyl)-1-ethyl-2- {[1-(3-fluorophenyl)- 1H-pyrazol-5- yl]methyl}-1H-benzimidazole 1H NMR (CDCl3): 7.91 (d, 1H), 7.64 (d, 1H), 7.27-7.50 (m, 5H), 7.10-7.18 (m, 3H), 6.77 (m, 1H), 6.20 (d, 1H), 4.36 (s, 2H), 4.02 (q, 2H), 1.25 (t, 3H)
    961
    Figure US20100267698A1-20101021-C01611
         		(1Z)-1-(2-{[1-(3- chlorophenyl)-1H- pyrazol-5-yl]methyl}- 1-ethyl-1H-benzimidazol- 5-yl)ethanone O- methyloxime 1H NMR (CDCl3): 7.94 (d, 1H), 7.73 (dd, 1H), 7.73 (dd, 1H) 7.63 (d, 1H), 7.52 (m, 1H), 7.38-7.40 (m, 3H), 7.27 (m, 1H), 6.19 (d, 1H), 4.32 (s, 2H), 3.87-4.10 (m, 5H), 2.30 (s, 3H), 1.22 (t, 3H.)
    962
    Figure US20100267698A1-20101021-C01612
         		2-{[1-(2,5- difluorophenyl)-1H- pyrazol-5-yl] methyl}-1-ethyl-5- (1,3,4-oxadiazol-2-yl)-1H- benzimidazole 425.10 [M + 1]
    963
    Figure US20100267698A1-20101021-C01613
         		(1E)-1-(1-ethyl-2-{[1-(3- fluorophenyl)- 1H-pyrazol-5- yl]methyl}-1H- benzimidazol-5- yl)ethanone oxime 1H NMR (CD3OD): 7.81 (d, 1H), 7.64-7.68 (m, 2H), 7.42-7.53 (m, 2H), 7.16-7.32 (m, 3H), 6.28 (d, 1H), 4.44 (s, 2H), 4.12 (q, 2H), 2.30 (s, 3H), 1.23 (t, 3H)
    964
    Figure US20100267698A1-20101021-C01614
         		(1E)-1-(1-ethyl-2-{[1-(3- fluorophenyl)-1H-pyrazol-5- yl]methyl}-1H- benzimidazol-5- yl)ethanone O-ethyloxime Syn and anti 1H NMR (CDCl3): 7.95 (d, 1H + H1), 7.73 (dd, 1H + H1), 7.63 (d, 1H + H1), 7.44 (m, 1H + H1), 7.24-7.33 (m, 3H + H1), [6.18 (d, 1H + H1), 7.13 (d, 1H + H1)], 4.33 (s, 2H), [4.26 (q, 2H), 4.13 (q, 2H1)], 3.98 (q, 2H + 2H1), [2.31 (s, 3H), 2.27 (s, 3H1)], [1.35 (t, 3H), 1.28 (t, 3H1)], 1.22 (t, 3H + H1)]
    965
    Figure US20100267698A1-20101021-C01615
         		2-{[1-(2,5- difluorophenyl)-1H- pyrazol-5-yl]methyl}- 1-ethyl-5-(5- pyridin-3-yl- 1,3,4-oxadiazol-2-yl)- 1H-benzimidazole 1H NMR (d6 DMSO): 9.40 (1H, s), 8.87 (1H, s), 8.63 (1H, d), 8.41 (1H, s), 8.20 (1H, d), 8.01 (1H, d), 7.76 (2H, m), 7.59 (1H, m), 7.48-7.41 (2H, m), 6.50 (1H, s). 4.61 (2H, s), 4.31 (2H, q), 1.19 (3H, t) 484.5 [M + 1]
    966
    Figure US20100267698A1-20101021-C01616
         		2-(1-ethyl-2-{[1- (3-fluorophenyl)- 1H-pyrazol- 5-yl]methyl}-1H- benzimidazol-5-yl)-4- methoxybenzaldehyde 1H NMR (CDCl3): 9.86 (s, 1H), 8.03 (d, 1H), 7.26-7.78 (m, 7H), 6.94-7.18 (m, 3H), 6.22 (dd, 1H), 4.38 (s, 2H), 4.04 (q, 2H), 3.91 (s, 3H), 1.27 (t, 3H)
    967
    Figure US20100267698A1-20101021-C01617
         		1-ethyl-2-{[1- (3-fluorophenyl)-1H- pyrazol-5-yl] methyl}-N,N-dimethyl 1H-benzimidazole-5- carboxamide 1H NMR (CDCl3): 7.78 (s, 1H) 7.63 (s, 1H), 7.10-7.48 (m, 6H), 6.18 (s, 1H), 4.35 (s, 2H), 4.00 (q, 2H), 3.13 (s, 3H), 3.05 (s, 3H), 1.22 (t, 3H)
    968
    Figure US20100267698A1-20101021-C01618
         		5-(3,5- dimethylisoxazol-4-yl)-1- ethyl-2-{[1- (3-fluorophenyl)-1H- pyrazol-5-yl]methyl}-1H- benzimidazole 1H NMR (CDCl3): 7.62-7.64 (m, 2H), 7.25-7.50 (m, 4H), 7.11-7.18 (m, 2H), 6.21 (d, 1H), 4.36 (s, 2H), 4.02 (q, 2H), 2.42 (s, 3H), 2.29 (s, 3H), 1.26 (t, 3H)
    969
    Figure US20100267698A1-20101021-C01619
         		5-bromo-2-{[1- (3-chlorophenyl)- 1H-pyrazol-5- yl]methyl}-1-ethyl- 1H-benzimidazole 1H NMR (CDCl3): 7.67 (d, 1H), 7.63 (d, 1H), 7.50 (m, 1H), 7.35-7.42 (m, 4H), 7.18 (d, 1H), 6.18 (d, 1H), 4.31 (s, 2H), 3.96 (q, 2H), 1.21 (t, 3H)
    970
    Figure US20100267698A1-20101021-C01620
         		2-{[1-(2,5- difluorophenyl)-1H- pyrazol-5-yl] methyl}-1-ethyl-5-(5- pyridin-4-yl-1,3,4- oxadiazol-2-yl)- 1H-benzimidazole Dihydrochloride 1H NMR (d6 DMSO): 8.98 (2H, m), 8.43 (1H, s), 8.35 (2H, m), 8.19 (1H, d), 7.99 (1H, d), 7.75 (1H, d), 7.59-7.569 1H, m), 7.50-7.41 (2H, m), 6.49 (1H, s), 4.58 (2H, s), 4.30 (2H, q), 1.19 (3H, t) 484.5 [M + 1]
    971
    Figure US20100267698A1-20101021-C01621
         		(1E)-1-(2-{[1-(3- chlorophenyl)-1H- pyrazol-5-yl] methyl}-1-ethyl-1H- benzimidazol-5-yl)ethanone oxime 1H NMR (CDCl3): 10.92 (br s, 1H), 8.58 (s, 1H), 7.52-7.64 (m, 3H), 7.29 (d, 1H), 6.20 (d, 1H), 4.36 (s, 2H), 3.97 (q, 2H), 2.38 (s, 3H), 1.21 (t, 3H)
    972
    Figure US20100267698A1-20101021-C01622
         		(1E)-1-(2-{[1-(3- chlorophenyl)-1H- pyrazol-5-yl] methyl}-1-ethyl-1H- benzimidazol-5-yl)ethanone O- ethyloxime Syn and anti 1H NMR (CDCl3): 7.95 (d, 1H + H1), 7.73 (dd, 1H + H1), 7.63 (d, 1H + H1), 7.44 (m, 1H + H1), 7.24-7.33 (m, 3H + H1), [6.18 (d, 1H + H1), 7.13 (d, 1H + H1)], 4.33 (s, 2H), [4.26 (q, 2H), 4.13 (q, 2H1)], 3.98 (q, 2H + 2H1), [2.31 (s, 3H), 2.27 (s, 3H1)], [1.35 (t, 3H), 1.28 (t, 3H1)], 1.22 (t, 3H + H1)]
    973
    Figure US20100267698A1-20101021-C01623
         		(1E)-1-(2-{[1-(3- chlorophenyl)-1H- pyrazol-5-yl] methyl}-1-ethyl-1H- benzimidazol-5- yl)ethanone O- (tert-butyl)oxime Syn and anti 1H NMR (CDCl3): 8.00 (d, 1H + H1), 7.75 (dd, 1H + H1), 7.63 (d, 1H + H1), 7.27 (d, 1H + H1), 7.38-7.40 (m, 3H + H1), 6.20 (d, 1H) [6.18 (d, 1H1)], 4.32 (s, 2H + H1), 3.99 (q, 2H + H1), 2.28 (s, 3H), [2.27 (s, 3H1]), 1.37 (s, 9H), [1.32 (s, 9H1]), 1.22 (t, 3H, t3H)
    974
    Figure US20100267698A1-20101021-C01624
         		(1E)-1-(2-{[1-(3- chlorophenyl)-1H- pyrazol-5-yl] methyl}-1-ethyl-1H- benzimidazol-5-yl) ethanone O- tetrahydro-2H- pyran-2-yloxime 1H NMR (CDCl3): 7.95 (d, 1H), 7.79 (dd, 1H), 7.63 (d, 1H), 7.52 (m, 1H), 7.37-7.40 (m, 3H), 7.26 (dd, 1H), 6.19 (d, 1H), 5.43 (m, 1H), 4.32 (s, 2H), 3.85-4.02 (m, 4H), 3.66 (m, 2H), 1.62-1.92 (m, 4H), 1.22 (t, 3H)
    975
    Figure US20100267698A1-20101021-C01625
         		(1-ethyl-2-{[1- (3-fluorophenyl)-1H- pyrazol-5-yl]methyl}-1H- benzimidazol-5- yl)acetonitrile 7.66 (m, 1H), 7.63 (d, 1H), 7.24-7.49 (m, 5H), 7.13 (m, 1H), 6.17 (d, 1H), 4.34 (s, 2H), 3.99 (q, 2H,), 3.87 (s, 2H), 1.22 (t, 3H)
    976
    Figure US20100267698A1-20101021-C01626
         		1-ethyl-2-{[1- (3-fluorophenyl)-1H- pyrazol-5-yl]methyl}- 5-(1,3,4- oxadiazol-2-yl)- 1H-benzimidazole 1H NMR (d6 DMSO): 8.17 (1H, s), 8.04-7.98 (2H, m), 7.71 (1H, d), 7.55-7.50 (2H, m), 7.45-7.43 (1H, m), 7.29-7.2491H, m), 6.40 (1H, d), 4.77 (2H, s), 4.36 (2H, q), 2.58 (3H, s), 1.24 (3H, t) 403.6 [M + 1]
    977
    Figure US20100267698A1-20101021-C01627
         		1-ethyl-2-{[1- (3-fluorophenyl)-1H- pyrazol-5-yl]methyl}- 5-(5-methyl- 1,3,4-oxadiazol-2- yl)-1H- benzimidazole 1H NMR (CD3OD): 8.79 (s, 1H), 8.31 (d, 1H), 7.69 (s, 1H), 7.60 (d, H), 7.46 (dd, 1H), 7.30 (d, 2H), 7.16-7.20 (m, 1H), 6.37 (s, 1H), 4.55 (s, 2H), 4.20 (q, 2H), 1.24 (t, 3H)
    978
    Figure US20100267698A1-20101021-C01628
         		1-ethyl-2-{[1- (3-fluorophenyl)-1H- pyrazol-5-yl]methyl}-1H- imidazo[4,5-c]pyridine 1H NMR (CD3OD): 8.79 (s, 1H), 8.31 (d, 1H), 7.69 (s, 1H), 7.60 (d, H), 7.46 (dd, 1H), 7.30 (d, 2H), 7.16-7.20 (m, 1H), 6.37 (s, 1 H), 4.55 (s, 2H), 4.20 (q, 2H), 1.24 (t, 3H)
    979
    Figure US20100267698A1-20101021-C01629
         		1-ethyl-2-{[1- (3-fluorophenyl)-1H- pyrazol-5-yl]methyl}-1H- benzimidazole-6- carbonitrile
    980
    Figure US20100267698A1-20101021-C01630
         		1-(1-ethyl-2-{[1- (3-fluorophenyl)-1H- pyrazol-5-yl]methyl}-1H- benzimidazol-5- yl)propan-1-one
    981
    Figure US20100267698A1-20101021-C01631
         		2-{[1-(3- fluorophenyl)-1H-pyrazol- 5-yl]methyl}-1- (2-methoxyethyl)- 1H-benzimidazole- 5-carbonitrile
    982
    Figure US20100267698A1-20101021-C01632
         		2-{[1-(3- fluorophenyl)-1H-pyrazol- 5-yl]methyl}-1- (3-methoxypropyl)- 1H-benzimidazole- 5-carbonitrile 390.1 [M + 1]; 388.3 [M − 1]
    983
    Figure US20100267698A1-20101021-C01633
         		1-(2-cyanoethyl)-2- {[1-(3- fluorophenyl)-1H-pyrazol-5- yl]methyl}-1H- benzimidazole-5- carbonitrile 371.3 [M + 1]
    984
    Figure US20100267698A1-20101021-C01634
         		1-ethyl-5-(5-ethyl- 1,3,4-oxadiazol- 2-yl)-2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}-1H- benzimidazole 1 H NMR (d6DMSO): 8.17 (1H, s), 8.01-7.94 (2H, m), 7.71 (1H, s), 7.53-7.51 (2H, m), 7.44-7.42 (1H, m), 7.28-7.24 (1H, m), 6.39 (1H, s), 4.73 (2H, s), 4.33 (2H, q), 2.94 (2H, q), 1.32 (3H, t), 1.23 (3H, t) 417.5 [M + 1]
    985
    Figure US20100267698A1-20101021-C01635
         		5-(1-ethyl-2-{[1- (3-fluorophenyl)- 1H-pyrazol-5-yl]methyl}-1H- benzimidazol-5-yl)-1,3,4- oxadiazol-2-amine 1 H NMR (d6DMSO): 8.17 (1H, s), 8.01-7.94 (2H, m), 7.71 (1H, s), 7.53-7.51 (2H, m), 7.44-7.42 (1H, m), 7.28-7.24 (1H, m), 6.39 (1H, s), 4.73 (2H, s), 4.33 (2H, q), 2.94 (2H, q), 1.32 (3H, t), 1.23 (3H, t) 417.5 [M + 1]
    986
    Figure US20100267698A1-20101021-C01636
         		2-[[1-(2,5- difluorophenyl)-1H- pyrazol-5-yl] (hydroxy)methyl]-1- ethyl-1H-benzimidazole-5- carbonitrile 1H NMR (CDCl3): 7.93 (d, 1H), 7.67 (d, 1H), 7.55 (dd, 1H), 7.40 (dd, 1H): 7.24 (m, 1H), 7.10-7.15 (m, 2H), 6.20 (d, 1H), 6.01 (s, 1H), 4.98 (br, S, 1H): 4.04 (q, 2H), 1.21 (t, 3H)
    987
    Figure US20100267698A1-20101021-C01637
         		1-ethyl-2-{[1- (3-fluorophenyl)-1H- pyrazol-5-yl]methyl}-1H- imidazo[4,5-b]pyridine 322.3 [M + 1]; 320.3 [M − 1]
    988
    Figure US20100267698A1-20101021-C01638
         		2-[[1-(2,5- difluorophenyl)-1H- pyrazol-5-yl] (fluoro)methyl]-1- ethyl-1H-benzimidazole-5- carbonitrile 1H NMR (CDCl3): 8.07 (d, 1H), 7.77 (d, 1H), 7.60 (dd, 1H), 7.47 (dd, 1H), 7.11-7.23 (m, 3H), 6.85 [d, 1H, J(H, F) = 47.4], 6.50 (t, 1H), 4.27 (m, 2H), 1.36 (t, 3H)
    989
    Figure US20100267698A1-20101021-C01639
         		1-ethyl-2-{[1- (3-fluorophenyl)-1H- pyrazol-5-yl]methyl}-5- (5-pyridin- 3-yl-1,3,4- oxadiazol-2-yl)-1H- benzimidazole 1H NMR (d6DMSO): 9.41 (1H, s), 8.88 (1H, d), 8.67 (1H, d), 8.46 (1H, s), 8.25 (1H, d), 8.10 (1H, d), 7.81-7.72 (2H, m), 7.55-7.46 (3H, m), 7.30-7.26 (1H, m), 6.43 (1H, s), 4.84 (2H, s), 4.40 (2H, q), 1.27 (3H, t)
    990
    Figure US20100267698A1-20101021-C01640
         		1-ethyl-2-{[1- (3-fluorophenyl)-1H- pyrazol-5-yl]methyl}-5- isoxazol-5- yl-1H-benzimidazole 1H NMR (CDCl3): 8.30 (d, 1H), 8.16 (dd, 1H), 7.76 (dd, 1H), 7.64 (d, 1H), 7.38-7.50 (m, 2H), 7.25-7.32 (m, 2H), 7.14 (m, 1H), 6.52 (d, 1H), 6.21 (d, 1H), 4.37 (s, 2H), 4.01 (q, 2H), 1.25 (t, 3H)
    991
    Figure US20100267698A1-20101021-C01641
         		1-ethyl-2-[[1- (3-fluorophenyl)-1H- pyrazol-5-yl] (hydroxy)methyl]-1H- benzimidazole 5-carbonitrile 1H NMR (CDCl3): 8.02 (dd, 1H), 7.47-7.62 (m 5H), 7.39 (d, 1H), 7.18 (m, 1H), 6.12 (d, 1H), 6.00 (s, 1H), 5.05 (brs, 1H), 3.83 (q, 2H), 1.08 (t, 3H)
    992
    Figure US20100267698A1-20101021-C01642
         		1-ethyl-2-{fluoro[1-(3- fluorophenyl)-1H-pyrazol-5- yl]methyl}-1H- benzimidazole-5- carbonitrile 1H NMR (CDCl3): 8.12 (dd, 1H), 7.73 (d, 1H), 7.62 (dd, 1H), 7.44-7.53 (m, 2H), 7.31-7.39 (m, 2H), 7.17 (m, 1H), 6.83 (d, 1H, JH, F = 46.8 MZ), 4.39 (m, 2H), 1.43 (t, 3H)
    993
    Figure US20100267698A1-20101021-C01643
         		methyl 5-[(5-cyano- 1-ethyl-1H- benzimidazol-2- yl)oxy]-1-(3- fluorophenyl)-1H- pyrazole-4- carboxylate 406.3 [M + 1]
    994
    Figure US20100267698A1-20101021-C01644
         		2-[[1-(2,5- difluorophenyl)-1H- pyrazol-5-yl](difluoro) methyl]-1- ethyl-1H-benzimidazole-5- carbonitrile 1H NMR (CDCl3): 8.09 (m, 1H), 7.84 (M, 1H), 7.64 (dd, 1H), 7.48 (dd, 1H), 6.96-7.18 (m, 3H), 6.85 (d, 1H), 4.33 (q, 2H), 1.38 (t, 3H)
    995
    Figure US20100267698A1-20101021-C01645
         		1-ethyl-2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}- 6-(1,3,4- oxadiazol-2-yl)- 1H-benzimidazole 389.2 [M + 1]
    996
    Figure US20100267698A1-20101021-C01646
         		1-ethyl-2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}- 6-(5-methyl- 1,3,4-oxadiazol- 2-yl)-1H- benzimidazole 403.3 [M + 1]
    997
    Figure US20100267698A1-20101021-C01647
         		1-(2-{[1-(3-fluorophenyl)-1H- pyrazol-5-yl]methyl}-1H- benzimidazol-1-yl)acetone 349.3 [M + 1]; 347.2 [M − 1]
    998
    Figure US20100267698A1-20101021-C01648
         		2-{difluoro[1- (3-fluorophenyl)-1H- pyrazol-5-yl] methyl}-1-ethyl-1H- benzimidazole-5-carbonitrile 1H NMR (CDCl3): 8.078 (m, 1H), 7.80 (m, 1H), 7.63 (dd, 1H), 7.48 (dd, 1H), 7.13-7.28 (m, 3H), 6.99 (m, 1H), 6.81 (d, 1H), 4.35 (q, 2H), 1.39 (t, 3H)
    999
    Figure US20100267698A1-20101021-C01649
         		2-{[1-(3-fluorophenyl)- 1H-pyrazol- 5-yl]methyl}-1-(3- fluoropropyl)-1H- benzimidazole 353.2 [M + 1]; 351.2 [M − 1]
    1000
    Figure US20100267698A1-20101021-C01650
         		1-(2-fluoroethyl)-2-{[1-(3- fluorophenyl)-1H-pyrazol-5- yl]methyl}-1H-benzimidazole 339.2 [M + 1]
    1001
    Figure US20100267698A1-20101021-C01651
         		4-(2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl)-1H- benzimidazol-1- yl)butanenitrile 360.2 [M + 1]; 358.3 [M − 1]
    1002
    Figure US20100267698A1-20101021-C01652
         		1-(1-ethyl-2-{[1- (3-fluorophenyl)- 1H-pyrazol-5-yl]methyl}-1H- benzimidazol-5-yl)-2,2,2- trifluoroethanone 417.2 [M + 1]
    1003
    Figure US20100267698A1-20101021-C01653
         		1-(2-{[1- (3-chlorophenyl)- 1H-pyrazol-5-yl] methyl}-1- ethyl-1H- benzimidazol-5-yl)-2,2,2- trifluoroethanone
    1004
    Figure US20100267698A1-20101021-C01654
         		5-chloro-2-[[1-(2,5- difluorophenyl)-1H-pyrazol-5- yl](difluoro) methyl]-1-ethyl-1H- benzimidazole
    1005
    Figure US20100267698A1-20101021-C01655
         		(1E)-1-(1-ethyl-2-{[1-(3- fluorophenyl)-1H- pyrazol-5- yl]methyl}-1H- benzimidazol-5- yl)ethanone O-(2- ethoxyethyl)oxime 1H NMR, δppm (CDC13): 7.95 (m, 1H), 7.72 (dd, 1H), 7.63 (d, 1H), 7.45 (m, 1H), 7.11-7.31 (m, 4H), 6.18 (d, 1H), 4.34-4.37 (m, 4H), 3.98 (q, 2H), 3.77 (t, 2H), 3.58 (q, 2H), 2.33 (s, 3H), 1.18-1.26 (m, 6H)
    1006
    Figure US20100267698A1-20101021-C01656
         		1-ethyl-2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}- N,N-dimethyl- 1H-benzimidazole- 6-carboxamide 1H NMR, δppm (CDC13: 7.72 (dd, 1H), 7.63 (d, 1H), 7.40-7.49 (m, 2H), 7.24-7.34 (m, 3H), 7.13 (d, 1H), 6.18 (d, 1H), 4.35 (s, 2H), 3.99 (q, 2H), 3.13 (s, 2H), 3.07 (s, 3H), 1.23 (t, 3H)
    1007
    Figure US20100267698A1-20101021-C01657
         		3-ethyl-2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}- 3H- imidazo[4,5-b]pyridine
    1008
    Figure US20100267698A1-20101021-C01658
         		2-{[1-(2,5- difluorophenyl)-1H- pyrazol-5-yl]methyl}- 3-ethyl-3H- imidazo[4,5-b]pyridine 1H NMR (CDC13): 8.19 (1H, d), 7.61 (1H, d), 7.48-7.44 (1H, m), 7.28-7.13 (4H, m), 6.14 (1H, m), 4.44 (2H, s), 3.93 (2H, q), 1.17 (3H, t), 356.2 [M + 1]
    1009
    Figure US20100267698A1-20101021-C01659
         		4-chloro-1-ethyl-2-{[1-(3- fluorophenyl)-1H-pyrazol-5- yl]methyl}-1H-imidazo[4,5- c]pyridine 1H nmr (CDCl3): 8.19 (1H, d), 7.61 (1H, d), 7.48-7.44 (1H, m), 7.28-7.13 (4H, m), 6.14 (1H, m), 4.44 (2H, s), 3.93 (2H, q), 1.17 (3H, t) 356.2 [M + 1]
    1010
    Figure US20100267698A1-20101021-C01660
         		3-ethyl-2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}- 5-methoxy- 3H-imidazo[4,5-b] pyridine 392.3 [M + 1]
    1011
    Figure US20100267698A1-20101021-C01661
         		2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}- 3-isopropyl-3H- imidazo[4,5-b]pyridine
    1012
    Figure US20100267698A1-20101021-C01662
         		1-(cyclopropylmethyl)- 2-{[1-(2,5- difluorophenyl)- 1H-pyrazol-5- yl]methyl}-1H-imidazo[4,5- b]pyridine H-1 NMR (CDCl3): 0.22 (m, 2H) 0.54 (m, 2H), 0.95 (m, 1H), 3.84 (d, 2H), 4.32 (s, 2H), 6.18 (s, 2H), 7.13-7.25 (m, 4H), 7.65 (m, 2H), 8.52 (d, 1H) 366 [M + 1]
    1013
    Figure US20100267698A1-20101021-C01663
         		1-(cyclopropylmethyl)- 2-{[1-(3- fluorophenyl)- 1H-pyrazol-5- yl]methyl}-1H-imidazo[4,5- b]pyridine H-1 NMR (CDCl3): 0.22 (m, 2H) 0.54 (m, 2H), 0.95 (m, 1H), 3.84 (d, 2H), 4.32 (s, 2H), 6.18 (s, 2H), 7.13-7.25 (m, 4H), 7.65 (m, 2H), 8.52 (d, 1H) 366 [M + 1]
    1014
    Figure US20100267698A1-20101021-C01664
         		2-{[1-(2,5- difluorophenyl)-1H- pyrazol-5-yl]methyl}- 1-propyl-1H- imidazo[4,5-b]pyridine H-1 NMR (CDCl3): 0.82 (t, 3H), 1.62 (h, 2H), 3.85 (t, 2H), 4.33 (s, 2H), 6.20 (s, 1H), 7.05-7.25 (m, 4H), 7.82 (m, 2H), 8.53 (d, 1H) 354 [M + 1]
    1015
    Figure US20100267698A1-20101021-C01665
         		2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}- 3-propyl-3H- imidazo[4,5-b]pyridine (CDCl3) 8.43 (dd, J = 4.8, 1.5 Hz, 1H), 8.10 (dd, J = 8.0, 1.4 Hz, 1H), 7.64 (d, J = 2.1 Hz, 1H), 7.47 (m, 1H), 7.23-7.34 (m, 3H), 7.12 (m, 1H), 6.31 (d, J = 1.8 Hz, 1H), 4.60 (s, 2H), 4.03 (t, J = 7.7 Hz, 2H), 1.65 (m, 2H), 0.84 (t, J = 7.5 Hz, m/z 366.2 [M + 1]
    1016
    Figure US20100267698A1-20101021-C01666
         		2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}- 5-methyl- 3-propyl-3H- imidazo[4,5-b]pyridine (CDCl3) 7.85 (d, J = 7.8 Hz, 1H), 7.64 (d, J = 1.5 Hz, 1H), 7.43 (m, 1H), 7.24-7.29 (m, 2H), 7.12 (m, 1H), 7.06 (d, J = 8.1 Hz, 1H), 6.22 (d, J = 1.5 Hz, 1H), 4.33 (s, 2H), 4.01 (t, J = 7.5 Hz, 2H), 2.64 (s, 3H), 1.67 (m, 2H), 0.84 (t, J = 7.3 Hz, 3H) m/z 350.3 [M + 1]
    1017
    Figure US20100267698A1-20101021-C01667
         		3-ethyl-2-({1-[3- (trifluoromethyl)phenyl]-1H- pyrazol-5-yl}methyl)-3H- imidazo[4,5-b]pyridine (CDCl3) 8.36 (dd, J = 4.8, 1.3 Hz, 1H), 7.98 (dd, J = 8.0, 1.3 Hz, 1H), 7.78 (m, 1H), 7.56-7.72 (m, 4H), 7.22 (dd, J = 8.1, 4.8 Hz, 1H), 6.29 (d, J = 1.5 Hz, 1H), 4.36 (s, 2H), 4.17 (q, J = 7.2 Hz, 2H), 1.27 (t, J = 7.2 Hz, 3H) m/z 372.2 [M + 1]
    1018
    Figure US20100267698A1-20101021-C01668
         		3-ethyl-2-{[1-(3- fluorophenyl)-1H- pyrazol-5-yl]methyl}- 5-methyl- 3H- imidazo[4,5-b]pyridine (CDCl3) 7.85 (d, J = 8.1 Hz, 1H), 7.64 (d, J = 1.8 Hz, 1H), 7.44 (m, 1H), 7.24-7.29 (m, 2H), 7.13 (m, 1H), 7.06 (d, J = 8.1 Hz, 1H), 6.21 (d, J = 1.5 Hz, 1H), 4.33 (s, 2H), 4.12 (q, J = 7.3 Hz, 2H), 2.65 (s, 3H), 1.25 (t, J = 7.3 Hz, 3H) m/z 336.2 [M + 1]
    1019
    Figure US20100267698A1-20101021-C01669
         		3-ethyl-2-{[1-(6- fluoropyridin-2-yl)- 1H-pyrazol-5-yl]methyl}- 5-methyl- 3H- imidazo[4,5-b]pyridine m/z 337.2 [M + 1]
    1020
    Figure US20100267698A1-20101021-C01670
         		8-{[1-(6- fluoropyridin-2-yl)- 1H-pyrazol-5-yl]methyl}- 2,9-dimethyl- 9H-purine (CDCl3) 8.81 (s, 1H), 7.87 (m, 2H), 7.69 (d, 1H), 6.67 (dd, 1H), 6.33 (s, 1H), 4.86 (s, 2H), 3.90 (s, 3H), 2.79 (s, 3H)
    1021
    Figure US20100267698A1-20101021-C01671
         		2-{[1-(6- fluoropyridin-2-yl)- 1H-pyrazol-5-yl]methyl}- 1,6-dimethyl- 1H-imidazo[4,5-c]pyridine (CDCl3) 8.82 (s, 1H), 7.88 (m, 1H), 7.84 (m, 1H), 7.67 (s, 1H), 7.11 (s, 1H), 6.69 (m, 1H), 6.27, (s, 1H), 4.85 (s, 2H), 3.81 (s, 3H), 2.66 (s, 3H) m/z 323 [M + 1]
    1022
    Figure US20100267698A1-20101021-C01672
         		8-{[1-(3- fluorophenyl)- 1H-pyrazol-5-yl]methyl}- 2,9-dimethyl- 9H-purine (CDCl3) 8.90 (s, 1H), 7.63 (d, 1H), 7.42 (m, 1H), 7.26 (m, 2H), 7.12 (m, 1H), 6.16 (d, 1H), 4.32 (s, 2H), 3.60 (s, 3H), 2.78 (s, 3H)
    1023
    Figure US20100267698A1-20101021-C01673
         		2-{[1-(3- fluorophenyl)- 1H-pyrazol-5-yl]methyl}- 1-methyl-6- (trifluoromethyl)-1H- benzimidazole (CDCl3) 7.79 (d, 1H), 7.59 (d, 2H), 7.54 (m, 2H), 7.30 (m, 2H), 7.16 (m, 1H), 6.12 (d, 1H), 4.38 (s, 2H), 3.61 (s, 3H),
    1024
    Figure US20100267698A1-20101021-C01674
         		9-ethyl-8-{[1-(6- fluoropyridin-2-yl)- 1H-pyrazol-5-yl]methyl}- 2-methyl- 9H-purine (CDCl3) 8.82 (s, 1H), 7.88 (m, 1H), 7.83 (q, 1H), 7.69 (d, 1H), 6.69 (m, 1H), 6.35 (s, 1H), 4.95 (s, 2H), 4.44 (q, 2H), 2.82 (s, 3H), 1.52 (t, 3H)
    1025
    Figure US20100267698A1-20101021-C01675
         		3-ethyl-2-{[1-(6- fluoropyridin-2-yl)- 1H-pyrazol-5-yl]methyl}- 3H- imidazo[4,5-c]pyridine (CDCl3) 8.78 (s, 1H), 8.38 (d, J = 3.9 Hz, 1H), 7.83-7.89 (m, 2H), 7.66 (s, 1H), 7.56 (d, J = 3.9 Hz, 1H), 7.25 (d, J = 9.9 Hz, 1H), 6.70 (d, J = 9.9 Hz, 1H), 4.81 (s, 2H), 4.39 (q, J = 5.4 Hz, 2H), 1.49 (t, J = 5.4 Hz, 3H) m/z 323 [M + 1]
    1026
    Figure US20100267698A1-20101021-C01676
         		1-ethyl-2-{[1-(3- fluoropyridin-2-yl)- 1H-pyrazol-5-yl]methyl}- 6-methyl- 1H-imidazo[4,5-c]pyridine (CDCl3) 8.82 (s, 1H), 8.28 (br d, J = 4.0 Hz, 1H), 7.70 (d, J = 1.6 Hz, 1H), 7.61 (m, 1H), 7.35 (m, 1H), 7.07 (s, 1H), 6.23 (s, 1H), 4.56 (s, 2H), 4.09 (q, J = 7.2 Hz, 2H), 2.65 (s, 3H), 1.25 (t, J = 7.2 Hz, 3H) m/z 337 [M + 1]
    1027
    Figure US20100267698A1-20101021-C01677
         		2-{[1-(6- fluoropyridin-2-yl)- 1H-pyrazol-5-yl]methyl}- 3-methyl-3H- imidazo[4,5-c]pyridine (CDCl3) 8.78 (br s, 1H), 8.38 (br s, 1H), 7.87 (m, 2H), 7.68 (s, 1H), 7.55 (br s, 1H), 7.25 (s, 1 H), 6.69 (br d, J = 7.5 Hz, 1H), 6.30 (s, 2H), 4.88 (s, 3H) m/z 309.2 [M + 1]
    1028
    Figure US20100267698A1-20101021-C01678
         		6-ethyl-2-{[1-(3- fluoropyridin-2-yl)- 1H-pyrazol-5-yl]methyl}- 1-methyl- 1H-imidazo[4,5-c]pyridine (CDCl3) 8.86 (s, 1H), 8.30 (d, J = 4.4 Hz, 1H), 7.71 (s, 1H), 7.62 (m, 1H), 7.36 (m, 1H), 7.07 (s, 1H), 6.18 (s, 1H), 4.58 (s, 2H), 3.64 (s, 3H), 2.94 (q, J = 7.6 Hz, 2H), 1.36 (t, J = 7.6 Hz, 3H) m/z 337 [M + 1]
    1029
    Figure US20100267698A1-20101021-C01679
         		2-{[1- (6-fluoropyridin-2-yl)- 1H-pyrazol-5-yl]methyl}- 3,5-dimethyl- 3H-imidazo[4,5-b]pyridine (CDCl3) 8.86 (s, 1H), 8.30 (d, J = 4.4 Hz, 1H), 7.71 (s, 1H), 7.62 (m, 1H), 7.36 (m, 1H), 7.07 (s, 1H), 6.18 (s, 1H), 4.58 (s, 2H), 3.64 (s, 3H), 2.94 (q, J = 7.6 Hz, 2H), 1.36 (t, J = 7.6 Hz 3H) m/z 337 [M + 1]
    1030
    Figure US20100267698A1-20101021-C01680
         		1-ethyl-2-{[1-(1,3- thiazol-2-yl)-1H- pyrazol-5-yl]methyl}-1H- imidazo[4,5-c]pyridine (CDCl3) 9.00 (s, 1H0, 8.43 (d, 1H), 7.63 (d, 1H), 7.43 (d, 1H), 7.15 (d, 1H), 7.06 (d, 1H), 6.25 (d, 1H), 4.99 (s, 2H), 4.25 (q, 2H), 1.36 (t, 3H)
    1031
    Figure US20100267698A1-20101021-C01681
         		1-ethyl-2-{1-[1-(1,3- thiazol-2-yl)-1H- pyrazol-5-yl]ethyl}-1H- imidazo[4,5-c]pyridine (CDCl3) 9.05 (s, 1H), 8.39 (d, 1H), 7.59 (d, 1H), 7.49 (d, 1H), 7.25 (d, 1H), 7.09 (d, 1H), 6.28 (d, 1H), 5.85 (q, 1H), 4.19 (q, 2H), 1.85 (d, 3H), 1.29 (t, 3H)
    1032
    Figure US20100267698A1-20101021-C01682
         		1-ethyl-2-{[1-(1,3- thiazol-2-yl)-1H- pyrazol-5-yl]methyl}-6- (trifluoromethyl)- 1H-imidazo[4,5-c] pyridine (CD3OD) 8.7 (s, 1H), 8.17 (s, 1H), 7.74 (d, J = 3.6 Hz, 1H), 7.33 (d, J = 3.6 Hz, 1H), 7.21 (d, J = 3.6 Hz, 1H), 7.21 (d, J = 3.6 Hz, 1H), 6.53 (s, 1H), 5.01 (s, 2H), 4.54 (q, J = 7.2 Hz, 2H), 1.52 (t,, J = 7.2 Hz, 3H)
    1033
    Figure US20100267698A1-20101021-C01683
         		3-ethyl-2-{[1-(1,3- thiazol-2-yl)-1H- pyrazol-5-yl]methyl}-3H- imidazo[4,5-c] pyridine (CD3OD) 9.42 (s, 1H), 8.44 (d, J = 6.6 Hz, 1 H), 7.9 (d, J = 6.3 Hz, 1H), 7.7 (s, 1H), 7.26 (d, J = 3.6 Hz, 1H), 7.20 (d J = 3.6 Hz, 1H), 6.63 (s, 1H), 5.09 (s, 2H), 4.68 (q, J = 7.5 Hz, 2H), 1.62 (t,, J = 7.5 Hz, 3H) m/z 311 [M + 1]
  • TABLE 8
    Cmp. MS
    # STRUCTURE IUPAC NAME NMR (m/z)
    1035
    Figure US20100267698A1-20101021-C01684
         		6-chloro-2-[(4-methyl-2-phenyl- 1H-imidazol-1-yl)methyl]-1- propyl-1H-benzimidazole 1 H NMR (CDCl3): 7.62-7.71 (m, 3H), 7.45-7.53 (m, 3H), 7.23-7.27 (m, 2H), 6.74 (q, 1H), 5.42 (s, 2H), 3.59 (t, 2H), 2.21 (d, 3H,), 1.37 (m, 2H), 0.70 (t, 3H)
    1036
    Figure US20100267698A1-20101021-C01685
         		2-[(4-methyl-2-phenyl-1H- imidazol-1-yl) methyl]-1-propyl- 1H-benzimidazole 1H NMR (CDCl3): 7.80 (m, 1H), 7.25-7.67 (m, 8H), 6.74 (q, 1H), 5.43 (s, 2H), 3.62 (t, 2H), 2.20 (d, 3H), 1.38 (m, 2H,), 0.69 (t, 3H)
    1037
    Figure US20100267698A1-20101021-C01686
         		6-chloro-2-{[4-chloro-2-(3- fluorophenyl)-1H-imidazol-1- yl]methyl}-1-propyl-1H- benzimidazole 1H NMR (CDCl3): 7.70 (d, 1H), 7.37-7.50 (m, 3H), 7.17-7.32 (m, 3H), 6.96 (s, 1H), 5.43 (s, 2H), 3.70 (t, 2H), 1.49 (m, 2H), 0.77 (t, 3H)
    1038
    Figure US20100267698A1-20101021-C01687
         		2-[(4-methyl-2-phenyl-1H- imidazol-1-yl) methyl]-3-propyl- 3H-imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.39 (dd, 1H), 8.06 (dd, 1H), 7.63-7.65 (m, 2H), 7.45-7.55 (m, 3H), 7.26 (m, 1H), 6.77 (q, 1H), 5.46 (s, 2H), 3.82 (t, 2H), 2.23 (d, 3H), 1.46 (m, 2H), 0.73 (t, 3H)
    1039
    Figure US20100267698A1-20101021-C01688
         		3-ethyl-2-{[2-(3- fluorophenyl)-4- methyl-1H-imidazol- 1-yl]methyl}- 3H-imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.39 (dd, 1H), 8.06 (dd, 1H), 7.38-7.50 (m, 3H), 7.12-7.29 (m, 2H), 6.77 (q, 1H), 5.46 (s, 2H), 3.98 (q, 2H), 2.22 (d, 3H), 1.08 (t, 3H)
    1040
    Figure US20100267698A1-20101021-C01689
         		2-{[2-(3- fluorophenyl)-4-methyl- 1H-imidazol-1- yl]methyl}-3- methyl-3H-imidazo [4,5-b]pyridine
    1041
    Figure US20100267698A1-20101021-C01690
         		2-{[2-(2,5- difluorophenyl)-4- methyl-1H-imidazol- 1-yl]methyl}- 3-methyl-3H-imidazo[4,5- b]pyridine
    1042
    Figure US20100267698A1-20101021-C01691
         		3-(cyclopropylmethyl)- 2-{[2-(2,5- difluorophenyl)-4- methyl-1H- imidazol-1-yl]methyl}-3H- imidazo[4,5-b]pyridine 1H NMR (CDCl3): 8.38 (dd, 1H), 8.04 (dd, 1H), 7.40 (m, 1H), 7.15-7.27 (m, 3H), 6.79 (d, 1H), 5.35 (s, 2H), 3.82 (d, 2H), 2.22 d, 3H), 0.75 (m, 1H), 0.39-0.44 (m, 2H), 0.23-0.28 (m, 2H)
    1043
    Figure US20100267698A1-20101021-C01692
         		1-ethyl-2-{[2-(3- fluorophenyl)-4- methyl-1H- imidazol-1-yl]methyl}-1H- benzimidazole-5- carbonitrile m/z 360 [M + 1]
    1044
    Figure US20100267698A1-20101021-C01693
         		1-ethyl-2-{[2-(3- fluorophenyl)-5- methyl-1H- imidazol-1-yl]methyl}-1H- benzimidazole-5- carbonitrile m/z 360 [M + 1]
    1045
    Figure US20100267698A1-20101021-C01694
         		2-[(2-methyl-4- phenyl-1,3- thiazol-5-yl)methyl]-1- propyl-1H- imidazo[4,5- c]pyridine (CDCl3) 9.06 (s, 1 H), 8.39 (d, J = 5.6 Hz, 1H), 7.61 (m, 2H), 7.47 (m, 2H), 7.41 (m, 1H), 7.20 (d, J = 5.6 Hz, 1H), 4.54 (s, 2H), 3.77 (t, J = 7.6 Hz, 2H), 2.68 (s, 3H), m/z 349 [M + 1]
    1046
    Figure US20100267698A1-20101021-C01695
         		5-bromo-1-ethyl-2-{[3-(3- fluorophenyl)-1H- pyrazol-4- yl]methyl}-1H- benzimidazole 401.0 [M + 1]
    1047
    Figure US20100267698A1-20101021-C01696
         		5-bromo-1-ethyl-2-{[5-(3- fluorophenyl)isoxazol-4- yl]methyl}-1H- benzimidazole (CDCl3) 8.40 (s, 1H), 7.88 (s, 1H), 7.31-7.52 (m, 4H), 7.18-7.22 (m, 2H), 4.14 (s, 2H), 4.04 (q, 2H), 1.18 (t, 3H)
    1048
    Figure US20100267698A1-20101021-C01697
         		5-bromo-1-ethyl-2-{[5-(3- fluorophenyl)-1-methyl-1H- pyrazol-4- yl]methyl}-1H- benzimidazole (CDCl3) 7.81 (s, 1H), 7.38-7.45 (m, 2H), 7.32 (dd, 1H), 7.00-7.18 (m, 4H), 4.00 (s, 2H), 3.90 (q, 2H), 3.78 (s, 3H), 1.15 (t, 3H)
    1049
    Figure US20100267698A1-20101021-C01698
         		5-bromo-1-ethyl-2-{[3-(3- fluorophenyl)-1-methyl-1H- pyrazol-4- yl]methyl}-1H- benzimidazole (CDCL3) 7.88 (s, 1H), 7.27-7.42 (m, 4H), 7.00-7.20 (m, 3H), 4.21 (s, 2H), 3.95 (t, 2H), 3.83 (s, 3H), 1.15 (t, 3H)
    1050
    Figure US20100267698A1-20101021-C01699
         		5-bromo-1-ethyl-2-{[4-(3- fluorophenyl)-1,3- thiazol-5- yl]methyl}-1H- benzimidazole (CDCl3) 8.78 (s, 1H), 7.91 (s, 1H), 7.38-7.48 (m, 4H), 7.11-7.19 (m, 2H), 4.59 (s, 2H), 3.90 (q, 2H), 1.12 (t, 3H)
    1051
    Figure US20100267698A1-20101021-C01700
         		1-ethyl-2-{[4-(6- fluoropyridin-2-yl)-1,3- thiazol-5-yl]methyl}-6- methyl- 1H-imidazo[4,5- c]pyridine (CDCl3) 8.92 (s, 1H), 8.75 (s, 1H), 8.16 (d, 1H), 7.90 (dd, 1H), 7.08 (s, 1H), 6.87 (d, 1H), 5.28 (s, 2H0, 4.32 (q, 2H), 2.63 (s, 3H), 1.32 (t, 3H)
    1052
    Figure US20100267698A1-20101021-C01701
         		2-{[(3,5-bis(3- fluorophenyl)isoxazol-4- yl]methyl}-1-ethyl-6- (trifluoromethyl)-1H- benzimidazole m/z 484 [M + 1]
    1053
    Figure US20100267698A1-20101021-C01702
         		2-(2,4′-bi-1,3-thiazol-5′- ylmethyl)-1-ethyl-1H- imidazo[4,5-c]pyridine (CDCl3) 9.05 (s, 1H0, 8.76 (s, 1H), 8.41 (d, 1H), 7.88 (d, 1H), 7.41 (d, 1H), 7.25 (d, 1H), 5.35 (s, 2H), 4.26 (q, 2H), 1.25 (t, 3H)
    1054
    Figure US20100267698A1-20101021-C01703
         		1-ethyl-2-{[2-(1,3-thiazol-2- yl)-4-(trifluoromethyl)-1H- imidazol-1-yl]methyl}-1H- imidazo[4,5-c]pyridine (CDCl3) 9.10 (s, 1H), 8.47 (d, J = 6.0 Hz, 1H), 7.88 (d, J = 3.2 Hz, 1H), 7.58 (s, 1H), 7.48 (d, J = 3.2 Hz, 1H), 7.31 (d, J = 5.6 Hz, 1H), 6.37 (s, 2H), 4.31 (q, J = 7.2 Hz, 2H), 1.24 (t, J = 7.2 Hz, 3H) m/z 379 [M + 1]
    1055
    Figure US20100267698A1-20101021-C01704
         		3-ethyl-6-isopropyl-2-{[2- (1,3-thiazol-2-yl)-4- (trifluoromethyl)-1H- imidazol-1-yl]methyl}-3H- imidazo[4,5-c]pyridine (CDCl3) 8.75 (s, 1H), 7.87 (d, J = 3.3 Hz, 1H), 7.59 (d, J = 0.9 Hz, 1H), 7.54 (s, 1H), 7.47 (d, J = 3.6 Hz, 1H), 6.32 (s, 2H), 4.38 (q, J = 7.2 Hz, 2H), 3.21 (sept, J = 6.9 Hz, 1H), 1.35 (d, J = 6.9 Hz, 6H) 1.30 (t J = 7 2 Hz m/z 421 [M + 1]
    1056
    Figure US20100267698A1-20101021-C01705
         		1-ethyl-6-isopropyl-2-{[2- (1,3-thiazol-2-yl)-4- (trifluoromethyl)-1H- imidazol-1-yl]methyl}-1H- imidazo[4,5-c]pyridine (CDCl3) 9.02 (s, 1H), 7.88 (d, J = 2.7 Hz, 1H), 7.55 (d, J = 0.9 Hz, 1H), 7.48 (d, J = 3.3 Hz, 1H), 7.13 (s, 1H), 6.34 (s, 2H), 4.28 (q, J = 7.2 Hz, 2H), 3.20 (sept, J = 6.9 Hz, 1H), 1.36 (d, J = 6.9 Hz, 6H), 1.22 (t, J = 7.2 Hz, m/z 421 [M + 1]
    • Example 51 Preparation of Radiolabeled Probe Compounds of the Invention
  • The compounds of the invention are prepared as radiolabeled probes by carrying out their synthesis using precursors comprising at least one atom that is a radioisotope. The radioisotope is preferably selected from of at least one of carbon (preferably 14C), hydrogen (preferably 3H), sulfur (preferably 35S), or iodine (preferably 125I). Such radiolabeled probes are conveniently synthesized by a radioisotope supplier specializing in custom synthesis of radiolabeled probe compounds. Such suppliers include Amersham Corporation, Arlington Heights, Ill.; Cambridge Isotope Laboratories, Inc. Andover, Mass.; SRI International, Menlo Park, Calif.; Wizard Laboratories, West Sacramento, Calif.; ChemSyn Laboratories, Lexena, Kans.; American Radiolabeled Chemicals, Inc., St. Louis, Mo.; and Moravek Biochemicals Inc., Brea, Calif.
  • Tritium labeled probe compounds are also conveniently prepared catalytically via platinum-catalyzed exchange in tritiated acetic acid, acid-catalyzed exchange in tritiated trifluoroacetic acid, or heterogeneous-catalyzed exchange with tritium gas. Such preparations are also conveniently carried out as a custom radiolabeling by any of the suppliers listed in the preceding paragraph using the compound of the invention as substrate. In addition, certain precursors may be subjected to tritium-halogen exchange with tritium gas, tritium gas reduction of unsaturated bonds, or reduction using sodium borotritide, as appropriate.
    • Example 52 Receptor Autoradiography
  • Receptor autoradiography (receptor mapping) is carried out in vitro as described by Kuhar in sections 8.1.1 to 8.1.9 of Current Protocols in Pharmacology (1998) John Wiley & Sons, New York, using radiolabeled compounds of the invention prepared as described in the preceding Example.
    • Example 53 Binding Assay
  • The high affinity and high selectivity of preferred compounds of the invention for the benzodiazepine site of the GABAA receptor can be confirmed using the binding assay described by Thomas and Tallman (J. Bio. Chem. 1981; 156:9838-9842, and J. Neurosci. 1983; 3:433-440).
  • Rat cortical tissue is dissected and homogenized in 25 volumes (w/v) of Buffer A (0.05 M Tris HCl buffer, pH 7.4 at 4° C.). The tissue homogenate is centrifuged in the cold (4° C.) at 20,000×g for 20 minutes. The supernatant is decanted, the pellet rehomogenized in the same volume of buffer, and centrifuged again at 20,000×g. The supernatant of this centrifugation step is decanted. The resulting pellet may be stored at −20° C. overnight. The pellet is then thawed and resuspended in 25 volumes of Buffer A (original wt/vol), centrifuged at 20,000×g and the supernatant decanted. This wash step is repeated once. The pellet is finally resuspended in 50 volumes of Buffer A.
  • Incubations contain 100 μl of tissue homogenate, 100 μl of radioligand, (0.5 nM 3H-Ro15-1788 [3H-Flumazenil], specific activity 80 Ci/mmol), and test compound or control (see below), and are brought to a total volume of 500 μl with Buffer A. Incubations are carried for 30 minutes at 4° C. and then rapidly filtered through Whatman GFE filters to separate free and bound ligand. Filters are washed twice with fresh Buffer A and counted in a liquid scintillation counter. Nonspecific binding (control) is determined by displacement of 3H Ro15-1788 with 10 μM Diazepam (Research Biochemicals International, Natick, Mass.). Data are collected in triplicate, averaged, and percent inhibition of total specific binding (Total Specific Binding Total—Nonspecific) is calculated for each compound.
  • A competition binding curve may obtained with up to 11 points spanning the compound concentration range from 10−12M to 10−5M obtained per curve by the method described above for determining percent inhibition. Ki values are calculated according the Cheng-Prussof equation. Each of the compounds disclosed in Tables 6-8 was tested in this fashion and each was found to have a Ki of <4 μM. Preferred compounds of the invention exhibit Ki values of less than 100 nM and more preferred compounds of the invention exhibit Ki values of less than 10 nM.
    • Example 54 Electrophysiology
  • The following assay can be used to determine if a compound of the invention acts as an agonist, an antagonist, or an inverse agonist at the benzodiazepine site of the GABAA receptor.
  • Assays are carried out as described in white and Gurley (NeuroReport 6: 1313-1316, 1995) and White, Gurley, Hartnett, Stirling, and Gregory (Receptors and Channels 3: 1-5, 1995) with modifications. Electrophysiological recordings are carried out using the two electrode voltage-clamp technique at a membrane holding potential of −70 mV. Xenopus Laevis oocytes are enzymatically isolated and injected with non-polyadenylated cRNA mixed in a ratio of 4:1:4 for α, β and γ subunits, respectively. Of the nine combinations of α, β and γ subunits described in the White et al. publications, preferred combinations are α1β2γ2, α2β3γ2, α3β3γ2, and α5β3γ2. Preferably all of the subunit cRNAs in each combination are human clones or all are rat clones. The sequence of each of these cloned subunits is available from GENBANK, e.g., human α1, GENBANK accession no. X14766, human α2, GENBANK accession no. A28100; human α3, GENBANK accession no. A28102; human α5, GENBANK accession no. A28104; human β2, GENBANK accession no. NM 021911; human β3, GENBANK accession no. M82919 and accession no. Z20136; human γ2, GENBANK accession no. X15376; rat α1, GENBANK accession no. L08490, rat α2, GENBANK accession no. L08491; rat α3, GENBANK accession no. L08492; rat α5, GENBANK accession no. L08494; rat P2, GENBANK accession no. X15467; rat β3, GENBANK accession no. X15468; and rat γ2, GENBANK accession no. L08497. For each subunit combination, sufficient message for each constituent subunit is injected to provide current amplitudes of >10 nA when 1 μM GABA is applied.
  • Compounds are evaluated against a GABA concentration that evokes <10% of the maximal evokable GABA current (e.g. 1 μM-9 μM). Each oocyte is exposed to increasing concentrations of a compound being evaluated (test compound) in order to evaluate a concentration/effect relationship. Test compound efficacy is calculated as a percent-change in current amplitude: 100*((Ic/I)−1), where Ic is the GABA evoked current amplitude observed in the presence of test compound and I is the GABA evoked current amplitude observed in the absence of the test compound.
  • Specificity of a test compound for the benzodiazepine site is determined following completion of a concentration/effect curve. After washing the oocyte sufficiently to remove previously applied test compound, the oocyte is exposed to GABA+1 μM RO15-1788, followed by exposure to GABA+1 μM RO15-1788+test compound. Percent change due to addition of compound is calculated as described above. Any percent change observed in the presence of RO15-1788 is subtracted from the percent changes in current amplitude observed in the absence of 1 μM RO15-1788. These net values are used for the calculation of average efficacy and EC50 values by standard methods. To evaluate average efficacy and EC50 values, the concentration/effect data are averaged across cells and fit to the logistic equation.
  • It is to be understood that the foregoing describes preferred embodiments of the present invention and that modifications may be made therein without departing from the scope of the present invention as set forth in the following claims.

Claims (24)

1. A compound of the formula:
Figure US20100267698A1-20101021-C01706
or a pharmaceutically acceptable salt thereof, wherein:
Z1 is nitrogen or CR1;
Z2 is nitrogen or CR2;
Z3 is nitrogen or CR3;
Z4 is nitrogen or CR4;
provided that no more than two of Z1, Z2, Z3, and Z4 are nitrogen;
R1, R2, R3, and R4 are independently selected from
i) hydrogen, halogen, hydroxy, nitro, cyano, amino, haloalkyl, and haloalkoxy,
ii) alkyl, alkoxy, cycloalkyl, alkenyl, alkynyl, (cycloalkyl)alkyl, —NH(R10), -N(R10)(R11) hydroxyalkyl, aminoalkyl, (R10)NHalkyl-, (R10)(R11)Nalkyl-, alkanoyl, alkoxycarbonyl, (heterocycloalkyl)alkyl, alkylsulfonyl, alkylthio, mono- or dialkylaminocarbonyl, heterocycloalkyl, aryl, and heteroaryl, each of which is optionally substituted with 1, 2, 3, or 4 of R20,
wherein R10 and R11 are independently selected at each occurrence from the group consisting of alkyl, alkenyl, alkynyl, alkoxy, cycloalkyl, (cycloalkyl)alkyl, aryl, arylalkyl, alkanoyl, and mono and dialkylaminoalkyl; and
iii) a group of the formula:
Figure US20100267698A1-20101021-C01707
where G is a bond, alkyl, —O—, —C(O)—, or —CH2C(═O)—, and
RA is a saturated, partially unsaturated, or aromatic carbocycle, consisting of 1 ring or 2 fused, pendant, or spiro rings, each ring containing 0, 1, or 2 heteroatoms independently chosen from N, S, and O, said saturated, partially unsaturated, or aromatic carbocycle is optionally substituted with 1, 2, 3, or 4 of R20,
iv) a group of the formula
Figure US20100267698A1-20101021-C01708
where J is N, CH, or C-alkyl, and
RB and RC are independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, alkoxy, cycloalkyl, (cycloalkyl)alkyl, heterocycloalkyl, aryl, arylalkyl, alkanoyl, heteroaryl, and mono and dialkylaminoalkyl, each of which is optionally substituted by 1 or 2 substituents independently chosen from halogen, hydroxy, cyano, amino, nitro, alkoxy, and alkyl;
RB and RC and the atom to which they are attached form a 4- to 10-membered monocyclic or bicyclic ring, which may contain:
a) one or more double bonds,
b) one or more of oxo, O, S, SO, SO2, or N—RD wherein RD is hydrogen, Ar1, alkyl, cycloalkyl, heterocycloalkyl, or Ar1alkyl; wherein Ar1 is aryl or heteroaryl, each of which is optionally substituted by 1 or 2 substituents independently chosen from halogen, hydroxy, cyano, amino, nitro, alkoxy, and alkyl, and/or
c) one or more substituents R20;
v) —OC(═O)RE, —C(═O)ORE, —C(═O)NH2, —C(═O)NHRE, —C(═O)NRERF, S(O)nRE, —S(O)nNH2, —S(O)nNHRE, —S(O)nNRERF, —NHC(═O)RE, —C(═NRE)RF, —HC═N—OH, —HC═N(alkoxy), —HC═N(alkyl), —NREC(═O)RF, NHS(O)mRE, and —NRES(O)mRF, where m is 0, 1 or 2, and
RE and RF are independently selected at each occurrence from alkyl, cycloalkyl, heterocycloalkyl, alkoxy, mono- or dialkylamino, aryl, or heteroaryl each of which is optionally substituted by 1, 2, or 3 of R20;
R20 is independently selected at each occurrence from the group consisting of: halogen; hydroxy; nitro; cyano; amino; alkyl; alkoxy optionally substituted with amino or mono- or dialkylamino; cycloalkyl; cycloalkylalkyl; cycloalkylalkoxy; alkenyl; alkynyl; haloalkyl; oxo; haloalkoxy; mono- and dialkylamino; aminoalkyl; and mono- and dialkylaminoalkyl;
R30 is independently selected at each occurrence from halogen, hydroxy, nitro, cyano, amino, alkyl, alkoxy optionally substituted with amino or mono- or dialkylamino, cycloalkyl, cycloalkylalkyl, cycloalkylalkoxy, heterocycloalkyl, alkenyl, alkynyl, haloalkyl, haloalkoxy, oxo, mono- and dialkylamino, aminoalkyl, and mono- and dialkylaminoalkyl;
R5 represents hydrogen or haloalkyl; or
R5 represents alkyl, cycloalkyl, or (cycloalkyl)alkyl, each of which may contain one or more double or triple bonds, and each of which is optionally substituted with 1, 2, or 3 of R30, or
R5 represents aryl, arylalkyl, heteroaryl, or heteroarylalkyl each of which is optionally substituted with 1, 2, or 3 substituents selected from the group consisting of haloalkyl, amino, —NH(R10), —N(R10)(R11), carboxamido, (R10)NHcarbonyl, (R10)(R11)Ncarbonyl, halogen, hydroxy, nitro, cyano, amino, alkyl, alkoxy optionally substituted with amino or mono- or dialkylamino, cycloalkyl, cycloalkylalkyl, cycloalkylalkoxy, heterocycloalkyl, alkenyl, alkynyl, haloalkyl, haloalkoxy, aminoalkyl, and mono- and dialkylaminoalkyl;
Q represents —C(R6)(R7) or oxygen,
with the proviso that Q is not oxygen when X2 is nitrogen;
R5 and R7 independently represent hydrogen, fluorine, or alkyl;
the group:
Figure US20100267698A1-20101021-C01709
represents a 5 to 7 membered heteroaryl or heterocycloalkyl ring containing up to 4 heteroatoms independently selected from nitrogen, sulfur, and oxygen, said 5 to 7 membered heteroaryl or heterocycloalkyl ring is substituted at each carbon atom by R, and substituted at each nitrogen atom available for substitution by R′, wherein
R is independently chosen at each occurrence from hydrogen, halogen, amino, alkyl, alkenyl, alkynyl, alkoxy, cycloalkyl, (cycloalkyl)alkyl, haloalkyl, haloalkoxy, carboxamido, and 3- to 7-membered carbocyclic or heterocyclic groups which are saturated, unsaturated, or aromatic, which may be further substituted with one or more substituents independently selected from halogen, oxo, hydroxy, alkyl, and alkoxy;
R′ is independently chosen at each occurrence from alkyl, hydrogen, cycloalkyl, cycloalkyl(alkyl), and 3- to 7-membered carbocyclic or heterocyclic groups which are saturated, unsaturated, or aromatic, which 3- to 7-membered carbocyclic or heterocyclic groups are optionally substituted with one or more substituents independently selected from halogen, oxo, hydroxy, alkyl, and alkoxy;
X1 and X2 independently represent nitrogen, carbon or CH;
Y is nitrogen, oxygen, carbon, —CH—, —CH2—, or absent; and
W represents aryl or heteroaryl, wherein the aryl or heteroaryl group is optionally substituted with up to 4 groups independently selected from R30, —CO2H, —C(═O)ORE, C(═O)NHRE, —C(═O)NRERF, —C(O)RE, and —S(O)mRE, —ORE, where R30 and RE are as defined above and m is 0, 1, or 2.
2-164. (canceled)
165. A pharmaceutical composition comprising a compound or salt according to claim 1 combined with at least one pharmaceutically acceptable carrier or excipient.
166. A method for altering the signal-transducing activity of a GABAA receptor, said method comprising contacting a cell expressing such a receptor with an amount of a compound or salt according to claim 1 sufficient to detectably alter the electrophysiology of the cell, wherein a detectable alteration of the electrophysiology of the cell indicates an alteration of the signal-transducing activity of GABAA receptors.
167. A method for altering the signal-transducing activity of a GABAA receptor, said method comprising contacting a cell expressing such receptors with an amount of a compound or salt according to claim 1 to detectably alter the chloride conductance in vitro of cell expressing GABAA receptors.
168. The method of claim 167 wherein the cell is recombinantly expresses a heterologous GABAA receptor and the alteration of the electrophysiology of the cell is detected by intracellular recording or patch clamp recording.
169. The method of claim 167 wherein the cell is a neuronal cell that is contacted in vivo in an animal, the cell is contacted with the compound or salt dissolved in a body fluid, and the alteration in the electrophysiology of the cell is detected as a change in the animal's behavior.
170. (canceled)
171. A method for altering the signal-transducing activity of a GABAA receptor, the method comprising exposing a cell expressing the GABAA receptor to an amount of a compound or salt according to claim 1 sufficient to inhibit RO15-1788 binding in vitro to cells expressing a human GABAA receptor.
172. A method for the treatment of anxiety, depression, a sleep disorder, schizophrenia, attention deficit-hyperactivity disorder, or for the enhancement of memory, comprising administering an effective amount of a compound or salt of claim 1 to a patient.
173. A method for demonstrating the presence of a GABAA receptor in a cell or tissue sample, said method comprising:
contacting a cell or tissue sample with a labeled compound or salt according to claim 1;
washing the cell or tissue sample to remove unbound labeled compound or salt; and
detecting the presence of labeled compound or salt in the cell or tissue sample.
174. The method of claim 173 in which the cell or tissue sample is a tissue section.
175. The method of claim 173 in which the labeled compound or salt contains a radioactive label or a directly or indirectly luminescent label.
176. The method of claim 173 in which the cell or tissue sample is a tissue section, labeled compound or salt contains a radioactive label or a directly or indirectly luminescent label, and the labeled compound or salt is detected autoradiographically to generate an autoradiogram.
177. A method for demonstrating the presence of a GABAA receptor in a tissue section comprising:
contacting the tissue section with a radiolabeled or luminescently labeled compound or salt according to claim 1 to yield a contacted tissue section;
washing the tissue section to remove unbound labeled compound or salt;
detecting the labeled compound or salt in the tissue section; and
comparing the exposure density of the tissue section with the exposure density of a second tissue section that has not been contacted with a compound or salt according to claim 1.
178-182. (canceled)
183. A process for preparing a compound of claim 1, where the compound of claim 1 has the formula
Figure US20100267698A1-20101021-C01710
comprising reacting a compound of Formula B
Figure US20100267698A1-20101021-C01711
with a compound of Formula C
Figure US20100267698A1-20101021-C01712
wherein:
Z1 is nitrogen or CR2;
Z2 is nitrogen or CR2;
Z3 is nitrogen or CR3;
Z4 is nitrogen or CR4;
provided that no more than two of Z2, Z2, Z3, and Z4 are nitrogen;
R2, R2, R3, and R4 are independently selected from
i) hydrogen, halogen, hydroxy, nitro, cyano, amino, haloalkyl, and haloalkoxy,
ii) alkyl, alkoxy, cycloalkyl, alkenyl, alkynyl, (cycloalkyl)alkyl, —NH(R10), —N(R10)(R11), hydroxyalkyl, aminoalkyl, (R10)NHalkyl, (R10)(R11)Nalkyl, alkanoyl, alkoxycarbonyl, (heterocycloalkyl)alkyl, alkylsulfonyl, alkylthio, mono- or dialkylaminocarbonyl, heterocycloalkyl, aryl, and heteroaryl, each of which is optionally substituted with 1, 2, 3, or 4 of R20,
wherein R10 and R11 are independently selected from the group consisting of alkyl, alkenyl, alkynyl, alkoxy, cycloalkyl, (cycloalkyl)alkyl, aryl, arylalkyl, alkanoyl, and mono and dialkylaminoalkyl; and
iii) a group of the formula:
Figure US20100267698A1-20101021-C01713
where G is a bond, alkyl, —O—, —C(═O)—, or —CH2C(═O)—, and
RA is a saturated, partially unsaturated, or aromatic carbocycle, consisting of 1 ring or 2 fused, pendant, or spiro rings, each ring containing 0, 1, or 2 heteroatoms independently chosen from N, S, and O, said saturated, partially unsaturated, or aromatic carbocycle is optionally substituted with 1, 2, 3, or 4 of R20, and
iv) a group of the formula
Figure US20100267698A1-20101021-C01714
where J is N, CH, or C-alkyl, and
RB and RC are independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, alkoxy, cycloalkyl, (cycloalkyl)alkyl, heterocycloalkyl, aryl, arylalkyl, alkanoyl, heteroaryl, and mono and dialkylaminoalkyl, each of which is optionally substituted by 1 or 2 substituents independently chosen from halogen, hydroxy, cyano, amino, nitro, alkoxy, and alkyl;
RB and RC and the atom to which they are attached form a 4- to 10-membered monocyclic or bicyclic ring, which may contain:
a) one or more double bonds,
b) one or more of oxo, O, S, SO, SO2, or N—RD wherein RD is hydrogen, Ar1, alkyl, cycloalkyl, heterocycloalkyl, or Ar1alkyl; wherein Ar1 is aryl or heteroaryl, each of which is optionally substituted by 1 or 2 substituents independently chosen from halogen, hydroxy, cyano, amino, nitro, alkoxy, and alkyl, and/or
c) one or more substituents R20;
v) —OC(═O)RE, —C(═O)ORE, —C(═O)NH2, —C(═O)NHRE, —C(═O)NRERF, S(O)nRE, —S(O)nNH2, —S(O)nNHRE, —S(O)nNRERF, —NHC(═O)RE, —C(═NRE)RF, —HC═N—OH, —HC═N(alkoxy), —HC═N(alkyl), —NREC(═O)RF, —NHS(O)mRE, and —NRES(O)mRF, where m is 0, 1 or 2, and
RE and RF are independently selected at each occurrence from alkyl, cycloalkyl, heterocycloalkyl, alkoxy, mono- or dialkylamino, aryl, or heteroaryl each of which is optionally substituted by 1, 2, or 3 of R30;
R20 is independently selected at each occurrence from the group consisting of: halogen; hydroxy; nitro; cyano; amino; alkyl; alkoxy optionally substituted with amino or mono- or dialkylamino; cycloalkyl; cycloalkylalkyl; cycloalkylalkoxy; alkenyl; alkynyl; haloalkyl; oxo; haloalkoxy; mono- and dialkylamino; aminoalkyl; and mono- and dialkylaminoalkyl;
R30 is independently selected at each occurrence from halogen, hydroxy, nitro, cyano, amino, alkyl, alkoxy optionally substituted with amino or mono- or dialkylamino, cycloalkyl, cycloalkylalkyl, cycloalkylalkoxy, heterocycloalkyl, alkenyl, alkynyl, haloalkyl, haloalkoxy, oxo, mono- and dialkylamino, aminoalkyl, and mono- and dialkylaminoalkyl;
R5 represents hydrogen or haloalkyl; or
R5 represents alkyl, cycloalkyl, or (cycloalkyl)alkyl, each of which may contain one or more double or triple bonds, and each of which is optionally substituted with 1, 2, or 3 of R30, or
R5 represents aryl, arylalkyl, heteroaryl, or heteroarylalkyl each of which is optionally substituted with 1, 2, or 3 substituents selected from the group consisting of haloalkyl, amino, —NH(R10), —N(R10)(R11), carboxamido, (R10)NHcarbonyl, (R10)(R11)Ncarbonyl, halogen, hydroxy, nitro, cyano, amino, alkyl, alkoxy optionally substituted with amino or mono- or dialkylamino, cycloalkyl, cycloalkylalkyl, cycloalkylalkoxy, heterocycloalkyl, alkenyl, alkynyl, haloalkyl, haloalkoxy, aminoalkyl, and mono- and dialkylaminoalkyl;
R6 and R7 independently represent hydrogen, fluorine, or alkyl;
R is independently chosen at each occurrence from hydrogen, halogen, amino, C2-C6alkyl, (C2-C6) alkenyl, (C2-C6) alkynyl, C2-C6alkoxy, (C3-C8) cycloalkyl, (C2-C8cycloalkyl)(C1-C4)alkyl, halo(C2-C6)alkyl, haloalkoxy, carboxamido, and 3- to 7-membered carbocyclic or heterocyclic groups which are saturated, unsaturated, or aromatic, which may be further substituted with one or more substituents independently selected from halogen, oxo, hydroxy, C2-4alkyl, and —O(C2-4alkyl); and
W represents aryl or heteroaryl, wherein the aryl or heteroaryl group is optionally substituted with up to 4 groups independently selected from R30, —CO2H, —C(═O)ORE, —C(═O)NHRE, —C(═O)NRERF, —C(O)RE, and —S(O)mRE, —ORE, where R30 and RE are as defined above and m is 0, 1, or 2.
184-210. (canceled)
211. A compound of the formula:
Figure US20100267698A1-20101021-C01715
wherein
Hal represents a halogen; and
Z2 is nitrogen or CR1;
Z2 is nitrogen or CR2;
Z3 is nitrogen or CR3;
Z4 is nitrogen or CR4;
provided that no more than two of Z1, Z2, Z3, and Z4 are nitrogen;
R1, R2, R3, and R4 are independently selected from
i) hydrogen, halogen, hydroxy, nitro, cyano, amino, haloalkyl, and haloalkoxy,
ii) alkyl, alkoxy, cycloalkyl, alkenyl, alkynyl, (cycloalkyl)alkyl, —NH(R10), —N(R10)(R11), hydroxyalkyl, aminoalkyl, (R10)NHalkyl-, (R10)(R11)Nalkyl-, alkanoyl, alkoxycarbonyl, (heterocycloalkyl)alkyl, alkylsulfonyl, alkylthio, mono- or dialkylaminocarbonyl, heterocycloalkyl, aryl, and heteroaryl, each of which is optionally substituted with 1, 2, 3, or 4 of R20,
wherein R10 and R11 are independently selected at each occurrence from the group consisting of alkyl, alkenyl, alkynyl, alkoxy, cycloalkyl, (cycloalkyl)alkyl, aryl, arylalkyl, alkanoyl, and mono and dialkylaminoalkyl; and
iii) a group of the formula:
Figure US20100267698A1-20101021-C01716
where G is a bond, alkyl, —O—, —C(═O)—, or —CH2C(═O)—, and
RA is a saturated, partially unsaturated, or aromatic carbocycle, consisting of 1 ring or 2 fused, pendant, or spiro rings, each ring containing 0, 1, or 2 heteroatoms independently chosen from N, S, and O, said saturated, partially unsaturated, or aromatic carbocycle is optionally substituted with 1, 2, 3, or 4 of R20,
iv) a group of the formula
Figure US20100267698A1-20101021-C01717
where J is N, CH, or C-alkyl, and
RB and RC are independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, alkoxy, cycloalkyl, (cycloalkyl)alkyl, heterocycloalkyl, aryl, arylalkyl, alkanoyl, heteroaryl, and mono and dialkylaminoalkyl,
each of which is optionally substituted by 1 or 2 substituents independently chosen from halogen, hydroxy, cyano, amino, nitro, alkoxy, and alkyl;
RB and RC and the atom to which they are attached form a 4- to 10-membered monocyclic or bicyclic ring, which may contain:
a) one or more double bonds,
b) one or more of oxo, O, S, SO, SO2, or N—RD wherein RD is hydrogen, Ar1, alkyl, cycloalkyl, heterocycloalkyl, or Ar1alkyl; wherein Ar1 is aryl or heteroaryl, each of which is optionally substituted by 1 or 2 substituents independently chosen from halogen, hydroxy, cyano, amino, nitro, alkoxy, and alkyl, and/or
c) one or more substituents R20;
v) —OC(═O)RE, —C(═O)ORE, —C(═O)NH2, —C(═O)NHRE, —C(═O)NRERF, —S(O)nRE, —S(O)nNH2, —S(O)nNHRE, —S(O)nNRERF, —NHC(═O)RE, —C(═NRE)RF, —HC═N—OH, —HC═N(alkoxy), —HC═N(alkyl), —NREC(═O)RF, —NHS(O)mRE, and —NRES(O)mRF, where m is 0, 1 or 2, and
RE and RF are independently selected at each occurrence from alkyl, cycloalkyl, heterocycloalkyl, alkoxy, mono- or dialkylamino, aryl, or heteroaryl each of which is optionally substituted by 1, 2, or 3 of R30;
R20 is independently selected at each occurrence from the group consisting of: halogen; hydroxy; nitro; cyano; amino; alkyl; alkoxy optionally substituted with amino or mono- or dialkylamino; cycloalkyl; cycloalkylalkyl; cycloalkylalkoxy; alkenyl; alkynyl; haloalkyl; oxo; haloalkoxy; mono- and dialkylamino; aminoalkyl; and mono- and dialkylaminoalkyl;
R30 is independently selected at each occurrence from halogen, hydroxy, nitro, cyano, amino, alkyl, alkoxy optionally substituted with amino or mono- or dialkylamino, cycloalkyl, cycloalkylalkyl, cycloalkylalkoxy, heterocycloalkyl, alkenyl, alkynyl, haloalkyl, haloalkoxy, oxo, mono- and dialkylamino, aminoalkyl, and mono- and dialkylaminoalkyl; and
R5 represents hydrogen or haloalkyl; or
R5 represents alkyl, cycloalkyl, or (cycloalkyl)alkyl, each of which may contain one or more double or triple bonds, and each of which is optionally substituted with 1, 2, or 3 of R30, or
R5 represents aryl, arylalkyl, heteroaryl, or heteroarylalkyl each of which is optionally substituted with 1, 2, or 3 substituents selected from the group consisting of haloalkyl, amino, —NH(R10), —N(R10)(R11), carboxamido, (R10)NHcarbonyl, (R10)(R11)Ncarbonyl, halogen, hydroxy, nitro, cyano, amino, alkyl, alkoxy optionally substituted with amino or mono- or dialkylamino, cycloalkyl, cycloalkylalkyl, cycloalkylalkoxy, heterocycloalkyl, alkenyl, alkynyl, haloalkyl, haloalkoxy, aminoalkyl, and mono- and dialkylaminoalkyl.
212. A compound of the formula:
Figure US20100267698A1-20101021-C01718
wherein
PROT represents a nitrogen protecting group; and
Z1 is nitrogen or CR2;
Z2 is nitrogen or CR2;
Z3 is nitrogen or CR3;
Z4 is nitrogen or CR4;
provided that no more than two of Z1, Z2, Z3, and Z4 are nitrogen;
R1, R2, R3, and R4 are independently selected from
i) hydrogen, halogen, hydroxy, nitro, cyano, amino, haloalkyl, and haloalkoxy,
ii) alkyl, alkoxy, cycloalkyl, alkenyl, alkynyl, (cycloalkyl)alkyl, —NH(R10), —N(R10)(R11), hydroxyalkyl, aminoalkyl, (R10)NHalkyl-, (R10)(R11)Nalkyl-, alkanoyl, alkoxycarbonyl, (heterocycloalkyl)alkyl, alkylsulfonyl, alkylthio, mono- or dialkylaminocarbonyl, heterocycloalkyl, aryl, and heteroaryl, each of which is optionally substituted with 1, 2, 3, or 4 of R20,
wherein R10 and R11 are independently selected at each occurrence from the group consisting of alkyl, alkenyl, alkynyl, alkoxy, cycloalkyl, (cycloalkyl)alkyl, aryl, arylalkyl, alkanoyl, and mono and dialkylaminoalkyl; and
iii) a group of the formula:
Figure US20100267698A1-20101021-C01719
where G is a bond, alkyl, —O—, —C(═O)—, or —CH2C(═O)—, and
RA is a saturated, partially unsaturated, or aromatic carbocycle, consisting of 1 ring or 2 fused, pendant, or spiro rings, each ring containing 0, 1, or 2 heteroatoms independently chosen from N, S, and O, said saturated, partially unsaturated, or aromatic carbocycle is optionally substituted with 1, 2, 3, or 4 of R20,
iv) a group of the formula
Figure US20100267698A1-20101021-C01720
where J is N, CH, or C-alkyl, and
RB and RC are independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, alkoxy, cycloalkyl, (cycloalkyl)alkyl, heterocycloalkyl, aryl, arylalkyl, alkanoyl, heteroaryl, and mono and dialkylaminoalkyl,
each of which is optionally substituted by 1 or 2 substituents independently chosen from halogen, hydroxy, cyano, amino, nitro, alkoxy, and alkyl;
RB and RC and the atom to which they are attached form a 4- to 10-membered monocyclic or bicyclic ring, which may contain:
a) one or more double bonds,
b) one or more of oxo, O, S, SO, SO2, or N—RD wherein RD is hydrogen, Ar1 alkyl, cycloalkyl, heterocycloalkyl, or Ar1alkyl; wherein Ar1 is aryl or heteroaryl, each of which is optionally substituted by 1 or 2 substituents independently chosen from halogen, hydroxy, cyano, amino, nitro, alkoxy, and alkyl, and/or
c) one or more substituents R20;
v) —OC(═O)RE, —C(═O)ORE, —C(═O)NH2, —C(═O)NHRE, —C(═O)NRERF, —S(O)nRE, —S(O)nNH2, —S(O)nNHRE, —S(O)nNRERF, —NHC(═O)RE, —C(═NRE)RF, —HC═N—OH, —HC═N(alkoxy), —HC═N(alkyl), —NREC(═O)RF, —NHS(O)mRE, and —NRES(O)mRF, where m is 0, 1 or 2, and
RE and RF are independently selected at each occurrence from alkyl, cycloalkyl, heterocycloalkyl, alkoxy, mono- or dialkylamino, aryl, or heteroaryl each of which is optionally substituted by 1, 2, or 3 of R30;
R20 is independently selected at each occurrence from the group consisting of: halogen; hydroxy; nitro; cyano; amino; alkyl; alkoxy optionally substituted with amino or mono- or dialkylamino; cycloalkyl; cycloalkylalkyl; cycloalkylalkoxy; alkenyl; alkynyl; haloalkyl; oxo; haloalkoxy; mono- and dialkylamino; aminoalkyl; and mono- and dialkylaminoalkyl;
R30 is independently selected at each occurrence from halogen, hydroxy, nitro, cyano, amino, alkyl, alkoxy optionally substituted with amino or mono- or dialkylamino, cycloalkyl, cycloalkylalkyl, cycloalkylalkoxy, heterocycloalkyl, alkenyl, alkynyl, haloalkyl, haloalkoxy, oxo, mono- and dialkylamino, aminoalkyl, and mono- and dialkylaminoalkyl; and
R5 represents hydrogen or haloalkyl; or
R5 represents alkyl, cycloalkyl, or (cycloalkyl)alkyl, each of which may contain one or more double or triple bonds, and each of which is optionally substituted with 1, 2, or 3 of R30, or
R5 represents aryl, arylalkyl, heteroaryl, or heteroarylalkyl each of which is optionally substituted with 1, 2, or 3 substituents selected from the group consisting of haloalkyl, amino, —NH(R10), —N(R10)(R11), carboxamido, (R10)NHcarbonyl, (R10)(R11)Ncarbonyl, halogen, hydroxy, nitro, cyano, amino, alkyl, alkoxy optionally substituted with amino or mono- or dialkylamino, cycloalkyl, cycloalkylalkyl, cycloalkylalkoxy, heterocycloalkyl, alkenyl, alkynyl, haloalkyl, haloalkoxy, aminoalkyl, and mono- and dialkylaminoalkyl.
213. A compound according to claim 212 wherein PROT is butoxycarbonyl.
214. A compound of the formula:
Figure US20100267698A1-20101021-C01721
wherein
RH represents a halogen or hydrogen;
Z1 is nitrogen or CR1;
Z2 is nitrogen or CR2;
Z3 is nitrogen or CR3;
Z4 is nitrogen or CR4;
provided that no more than two of Z1, Z2, Z3, and Z4 are nitrogen;
R1, R2, R3, and R4 are independently selected from
i) hydrogen, halogen, hydroxy, nitro, cyano, amino, haloalkyl, and haloalkoxy,
ii) alkyl, alkoxy, cycloalkyl, alkenyl, alkynyl, (cycloalkyl)alkyl, —NH(R10), —N(R10)(R11), hydroxyalkyl, aminoalkyl, (R10)NHalkyl-, (R10)(Rn)Nalkyl-, alkanoyl, alkoxycarbonyl, (heterocycloalkyl)alkyl, alkylsulfonyl, alkylthio, mono- or dialkylaminocarbonyl, heterocycloalkyl, aryl, and heteroaryl, each of which is optionally substituted with 1, 2, 3, or 4 of R20,
wherein R10 and R11 are independently selected at each occurrence from the group consisting of alkyl, alkenyl, alkynyl, alkoxy, cycloalkyl, (cycloalkyl)alkyl, aryl, arylalkyl, alkanoyl, and mono and dialkylaminoalkyl; and
iii) a group of the formula:
Figure US20100267698A1-20101021-C01722
where G is a bond, alkyl, —O—, —C(═O)—, or —CH2C(═O)—, and
RA is a saturated, partially unsaturated, or aromatic carbocycle, consisting of 1 ring or 2 fused, pendant, or spiro rings, each ring containing 0, 1, or 2 heteroatoms independently chosen from N, S, and O, said saturated, partially unsaturated, or aromatic carbocycle is optionally substituted with 1, 2, 3, or 4 of R20,
iv) a group of the formula
Figure US20100267698A1-20101021-C01723
where J is N, CH, or C-alkyl, and
RB and RC are independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, alkoxy, cycloalkyl, (cycloalkyl)alkyl, heterocycloalkyl, aryl, arylalkyl, alkanoyl, heteroaryl, and mono and dialkylaminoalkyl,
each of which is optionally substituted by 1 or 2 substituents independently chosen from halogen, hydroxy, cyano, amino, nitro, alkoxy, and alkyl;
RE, and Rc and the atom to which they are attached form a 4- to 10-membered monocyclic or bicyclic ring, which may contain:
a) one or more double bonds,
b) one or more of oxo, O, S, SO, SO2, or N—RD wherein RD is hydrogen, Ar1, alkyl, cycloalkyl, heterocycloalkyl, or Ar1alkyl; wherein Ar1 is aryl or heteroaryl, each of which is optionally substituted by 1 or 2 substituents independently chosen from halogen, hydroxy, cyano, amino, nitro, alkoxy, and alkyl, and/or
c) one or more substituents R20;
v) —OC(═O)RE, —C(═O)ORE, —C(═O)NH2, —C(═O)NHRE, —C(═O)NRERF, —S(O)nRE, —S(O)nNH2, —S(O)nNHRE, —S(O)nNRERF, —NHC(═O)RE, —C(═NRE)RF, —HC═N—OH, —HC═N(alkoxy), —HC═N(alkyl), —NREC(═O)RF, —NHS(O)mRE, and —NRES(O)mRF, where m is 0, 1 or 2, and
RE and RF are independently selected at each occurrence from alkyl, cycloalkyl, heterocycloalkyl, alkoxy, mono- or dialkylamino, aryl, or heteroaryl each of which is optionally substituted by 1, 2, or 3 of R30;
R20 is independently selected at each occurrence from the group consisting of: halogen; hydroxy; nitro; cyano; amino; alkyl; alkoxy optionally substituted with amino or mono- or dialkylamino; cycloalkyl; cycloalkylalkyl; cycloalkylalkoxy; alkenyl; alkynyl; haloalkyl; oxo; haloalkoxy; mono- and dialkylamino; aminoalkyl; and mono- and dialkylaminoalkyl;
R30 is independently selected at each occurrence from halogen, hydroxy, nitro, cyano, amino, alkyl, alkoxy optionally substituted with amino or mono- or dialkylamino, cycloalkyl, cycloalkylalkyl, cycloalkylalkoxy, heterocycloalkyl, alkenyl, alkynyl, haloalkyl, haloalkoxy, oxo, mono- and dialkylamino, aminoalkyl, and mono- and dialkylaminoalkyl;
R5 represents hydrogen or haloalkyl; or
R5 represents alkyl, cycloalkyl, or (cycloalkyl)alkyl, each of which may contain one or more double or triple bonds, and each of which is optionally substituted with 1, 2, or 3 of R30, or
R5 represents aryl, arylalkyl, heteroaryl, or heteroarylalkyl each of which is optionally substituted with 1, 2, or 3 substituents selected from the group consisting of haloalkyl, amino, —NH(R10), —N(R10)(R11), carboxamido, (R10)NHcarbonyl, (R10)(R11)Ncarbonyl, halogen, hydroxy, nitro, cyano, amino, alkyl, alkoxy optionally substituted with amino or mono- or dialkylamino, cycloalkyl, cycloalkylalkyl, cycloalkylalkoxy, heterocycloalkyl, alkenyl, alkynyl, haloalkyl, haloalkoxy, aminoalkyl, and mono- and dialkylaminoalkyl; and
W represents aryl or heteroaryl, wherein the aryl or heteroaryl group is optionally substituted with up to 4 groups independently selected from R30, —CO2H, —C(═O)ORE, —C(═O)NHRE, —C(═O)NRERF, —C(O)RE, and —S(O)mRE, —ORE, where R30 and RE are as defined above; and m is 0, 1, or 2.
215. A compound according to claim 214 wherein RH is hydrogen.
216. A compound according to claim 214 wherein RH is halogen.
US12/652,435 2000-12-21 2010-01-05 Benzimidazole and Pyridylimidazole Derivatives Abandoned US20100267698A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12/652,435 US20100267698A1 (en) 2000-12-21 2010-01-05 Benzimidazole and Pyridylimidazole Derivatives

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US25749200P 2000-12-21 2000-12-21
US10/038,069 US6916819B2 (en) 2000-12-21 2001-12-21 Benzimidazole and pyridylimidazole derivatives
US11/179,458 US7300945B2 (en) 2000-12-21 2005-07-12 Benzimidazole and pyridylimidazole derivatives
US11/942,480 US7642267B2 (en) 2000-12-21 2007-11-19 Benzimidazole and pyridylimidazole derivatives
US12/652,435 US20100267698A1 (en) 2000-12-21 2010-01-05 Benzimidazole and Pyridylimidazole Derivatives

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US11/942,480 Division US7642267B2 (en) 2000-12-21 2007-11-19 Benzimidazole and pyridylimidazole derivatives

Publications (1)

Publication Number Publication Date
US20100267698A1 true US20100267698A1 (en) 2010-10-21

Family

ID=22976520

Family Applications (4)

Application Number Title Priority Date Filing Date
US10/038,069 Expired - Fee Related US6916819B2 (en) 2000-12-21 2001-12-21 Benzimidazole and pyridylimidazole derivatives
US11/179,458 Expired - Fee Related US7300945B2 (en) 2000-12-21 2005-07-12 Benzimidazole and pyridylimidazole derivatives
US11/942,480 Expired - Fee Related US7642267B2 (en) 2000-12-21 2007-11-19 Benzimidazole and pyridylimidazole derivatives
US12/652,435 Abandoned US20100267698A1 (en) 2000-12-21 2010-01-05 Benzimidazole and Pyridylimidazole Derivatives

Family Applications Before (3)

Application Number Title Priority Date Filing Date
US10/038,069 Expired - Fee Related US6916819B2 (en) 2000-12-21 2001-12-21 Benzimidazole and pyridylimidazole derivatives
US11/179,458 Expired - Fee Related US7300945B2 (en) 2000-12-21 2005-07-12 Benzimidazole and pyridylimidazole derivatives
US11/942,480 Expired - Fee Related US7642267B2 (en) 2000-12-21 2007-11-19 Benzimidazole and pyridylimidazole derivatives

Country Status (40)

Country Link
US (4) US6916819B2 (en)
EP (1) EP1368342B3 (en)
JP (1) JP2004536782A (en)
KR (1) KR100854174B1 (en)
CN (1) CN1553909A (en)
AP (1) AP1503A (en)
AR (1) AR035774A1 (en)
AT (1) ATE304008T1 (en)
AU (2) AU2002232768B2 (en)
BG (1) BG107899A (en)
BR (1) BR0116385A (en)
CA (1) CA2431592A1 (en)
CO (1) CO5390086A1 (en)
CZ (1) CZ20031676A3 (en)
DE (1) DE60113302T2 (en)
EA (1) EA007157B1 (en)
EC (1) ECSP034656A (en)
EE (1) EE05108B1 (en)
EG (1) EG24590A (en)
ES (1) ES2256325T3 (en)
HR (1) HRP20030483A9 (en)
HU (1) HUP0303849A3 (en)
IL (1) IL156294A0 (en)
IS (1) IS6855A (en)
MA (1) MA26974A1 (en)
MX (1) MXPA03005493A (en)
MY (1) MY141733A (en)
NO (1) NO326558B1 (en)
NZ (1) NZ526330A (en)
OA (1) OA12539A (en)
PA (1) PA8535601A1 (en)
PE (1) PE20020720A1 (en)
PL (1) PL366243A1 (en)
SK (1) SK7672003A3 (en)
TW (1) TWI304402B (en)
UA (1) UA75902C2 (en)
UY (1) UY27095A1 (en)
WO (1) WO2002050062A2 (en)
YU (1) YU51703A (en)
ZA (1) ZA200304544B (en)

Families Citing this family (107)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI248936B (en) * 2001-03-21 2006-02-11 Merck Sharp & Dohme Imidazo-pyrimidine derivatives as ligands for GABA receptors
IL159811A0 (en) * 2001-07-13 2004-06-20 Neurogen Corp Heteroaryl substituted fused bicyclic heteroaryl compounds as gabaa receptor ligands
WO2003060475A2 (en) 2002-01-10 2003-07-24 Neurogen Corporation Melanin concentrating hormone receptor ligands: substituted 2-(4-benzyl-piperazin-1-ylmethyl)- and 2-(4-benzyl-diazepan-1-ylmethyl)-1h-benzoimidazole analogues
EP1465888A2 (en) 2002-01-10 2004-10-13 Neurogen Corporation Melanin concentrating hormone receptor ligands: substituted benzoimidazole analogues
AU2003245259A1 (en) * 2002-05-02 2003-11-17 Neurogen Corporation Substituted imidazole derivatives: gabaa receptor ligands
AU2003301864A1 (en) * 2002-05-08 2004-06-07 Neurogen Corporation Substituted imidazolylmethyl pyridine and pyrazine derivatives and their use as gabaa receptor ligands
ATE333455T1 (en) * 2002-05-17 2006-08-15 Neurogen Corp SUBSTITUTED RING-CONDENSED IMIDAZOLE DERIVATIVES: GABAA RECEPTOR LIGANDS
US20040063938A1 (en) * 2002-09-30 2004-04-01 Pfizer Inc Process for preparing haloalkyl pyrimidines
CN100579579C (en) * 2002-10-01 2010-01-13 诺华疫苗和诊断公司 Anti-cancer and anti-infectious disease compositions and methods of use thereof
WO2004041808A1 (en) * 2002-11-08 2004-05-21 Neurogen Corporation 4-imidazol-1-ylmethyl-pyrimidine derivatives_as ligands for gabaa receptors
WO2004054515A2 (en) * 2002-12-13 2004-07-01 Smithkline Beecham Corporation Thrombopoietin mimetics
US7169801B2 (en) * 2003-03-17 2007-01-30 Takeda San Diego, Inc. Histone deacetylase inhibitors
SE0301699D0 (en) * 2003-06-10 2003-06-10 Astrazeneca Ab Benzimidazole derivatives, compositions containing them, preparation thereof and uses thereof
EP1880998A1 (en) * 2003-07-25 2008-01-23 Neurogen Corporation Imidazo-pyrimidines and triazolo-pyrimidines: benzodiazepine receptor ligands
PT1648899E (en) * 2003-07-25 2007-03-30 Neurogen Corp Imidazo-pyrimidines and triazolo-pyrimidines: benzodiazepine receptor ligands
ZA200600037B (en) * 2003-07-25 2007-04-25 Neurogen Corp Imidazo-pyrimidines and triazolo-pyrimidines: Benzodiazepine receptor ligands
DE602004027932D1 (en) 2003-09-22 2010-08-12 S Bio Pte Ltd Benzimidazole derivatives: Preparation and pharmaceutical applications
HUP0304101A3 (en) * 2003-12-22 2008-10-28 Sanofi Aventis Pyrazole derivatives, process for producing them, their use, pharmaceutical compositions containing them and their intermediates
WO2005121132A1 (en) * 2004-06-11 2005-12-22 Shionogi & Co., Ltd. Fused heterocyclic compound having anti-hcv effect
US7534796B2 (en) 2005-02-18 2009-05-19 Wyeth Imidazo[4,5-b]pyridine antagonists of gonadotropin releasing hormone receptor
US7538113B2 (en) 2005-02-18 2009-05-26 Wyeth 4-substituted imidazo[4,5-c]pyridine antagonists of gonadotropin releasing hormone receptor
US7582634B2 (en) 2005-02-18 2009-09-01 Wyeth 7-substituted imidazo[4,5-c]pyridine antagonists of gonadotropin releasing hormone receptor
US7531542B2 (en) 2005-05-18 2009-05-12 Wyeth Benzooxazole and benzothiazole antagonists of gonadotropin releasing hormone receptor
US7582636B2 (en) 2005-05-26 2009-09-01 Wyeth Piperazinylimidazopyridine and piperazinyltriazolopyridine antagonists of Gonadotropin Releasing Hormone receptor
BRPI0617306A2 (en) * 2005-10-11 2011-07-19 Hoffmann La Roche isoxazole derivatives
CN101346372B (en) 2005-12-23 2011-09-28 弗·哈夫曼-拉罗切有限公司 Aryl-isoxazol-4-yl-oxadiazole derivatives
KR101121372B1 (en) 2005-12-27 2012-04-12 에프. 호프만-라 로슈 아게 Aryl-isoxazol-4-yl-imidazole derivatives
BRPI0706560A2 (en) 2006-01-17 2011-03-29 Hoffmann La Roche aryl-isoxazol-4-yl-imidazo [1,2-a] pyridine derivatives useful for the treatment of alzheimer's disease via gaba receptors
EP2029559B1 (en) 2006-05-31 2009-08-05 F. Hoffmann-Roche AG Aryl-4-ethynyl-isoxazole derivatives
TW200808772A (en) * 2006-06-13 2008-02-16 Astrazeneca Ab Therapeutic compounds
WO2008016123A1 (en) * 2006-08-03 2008-02-07 Takeda Pharmaceutical Company Limited GSK-3β INHIBITOR
WO2008051533A2 (en) * 2006-10-25 2008-05-02 Takeda Pharmaceutical Company Limited Benzimidazole compounds
ATE519760T1 (en) 2007-06-22 2011-08-15 Hoffmann La Roche ISOXAZOLIMIDAZOLE DERIVATIVES
TW200911803A (en) * 2007-07-16 2009-03-16 Organon Nv 6-phenyl-1H-imidazo [4,5-c] pyridine-4-carbonitrile derivatives
WO2009023126A2 (en) * 2007-08-10 2009-02-19 Cortex Pharmaceuticals, Inc. Bicyclic amides for enhancing glutamatergic synaptic responses
WO2009071464A1 (en) 2007-12-04 2009-06-11 F. Hoffmann-La Roche Ag Isoxazolo-pyrazine derivatives
US7943619B2 (en) 2007-12-04 2011-05-17 Hoffmann-La Roche Inc. Isoxazolo-pyridazine derivatives
US7902201B2 (en) 2007-12-04 2011-03-08 Hoffmann-La Roche Inc. Isoxazolo-pyrazine derivatives
TW201033201A (en) 2009-02-19 2010-09-16 Hoffmann La Roche Isoxazole-isoxazole and isoxazole-isothiazole derivatives
US8389550B2 (en) 2009-02-25 2013-03-05 Hoffmann-La Roche Inc. Isoxazoles / O-pyridines with ethyl and ethenyl linker
US8222246B2 (en) 2009-04-02 2012-07-17 Hoffmann-La Roche Inc. Substituted isoxazoles
US8227461B2 (en) 2009-04-30 2012-07-24 Hoffmann-La Roche Inc. Isoxazoles
AU2010244553A1 (en) 2009-05-05 2011-12-01 F. Hoffmann-La Roche Ag Isoxazole-pyridine derivatives
JP6026273B2 (en) 2009-05-05 2016-11-16 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Isoxazole-pyridazine derivatives
MX2011011273A (en) 2009-05-05 2011-11-04 Hoffmann La Roche Isoxazole-pyrazole derivatives.
CN102414208B (en) 2009-05-05 2014-11-05 霍夫曼-拉罗奇有限公司 Isoxazole-thiazole derivatives as GABAA a receptor inverse agonists for use in the treatment of cognitive disorders
EP2427457B1 (en) 2009-05-07 2013-07-17 F.Hoffmann-La Roche Ag Isoxazole-pyridine derivatives as gaba modulators
CN103209981B (en) * 2010-09-10 2016-12-28 盐野义制药株式会社 There is the heterocyclic fused imdazole derivatives of AMPK activation
US20130196990A1 (en) 2010-10-06 2013-08-01 Junya Qu Benzimidazole Derivatives As PI3 Kinase Inhibitors
NO3034079T3 (en) 2010-11-15 2018-06-09
BR112013021236B1 (en) * 2011-02-25 2021-05-25 Merck Sharp & Dohme Corp benzimidazole derivative compound, and, composition
ES2786298T3 (en) 2011-03-03 2020-10-09 Zalicus Pharmaceuticals Ltd Benzimidazole sodium channel inhibitors
CN102653531B (en) * 2011-03-04 2014-08-13 上海爱普植物科技有限公司 Synthesis method of massoia lactone
AU2012293611B2 (en) * 2011-08-11 2017-02-09 Bayer Cropscience Ag 1,2,4-triazolyl-substituted keto-enols
US8785435B2 (en) 2011-10-20 2014-07-22 Hoffmann-La Roche Inc. Solid forms
GB201119538D0 (en) * 2011-11-10 2011-12-21 Viral Ltd Pharmaceutical compounds
HK1201270A1 (en) 2012-01-11 2015-08-28 Bayer Intellectual Property Gmbh Tetrazol-5-yl- and triazol-5-yl-aryl compounds and use thereof as herbicides
US20150374705A1 (en) 2012-02-14 2015-12-31 Shanghai Institues for Biological Sciences Substances for treatment or relief of pain
US9296725B2 (en) 2012-05-24 2016-03-29 Cellzome Limited Heterocyclyl pyrimidine analogues as TYK2 inhibitors
JP6230344B2 (en) * 2013-09-06 2017-11-15 株式会社東芝 Steam turbine plant
WO2015052173A1 (en) 2013-10-10 2015-04-16 Basf Se Tetrazole and triazole compounds and their use as herbicides
WO2015052178A1 (en) 2013-10-10 2015-04-16 Basf Se 1,2,5-oxadiazole compounds and their use as herbicides
NZ722077A (en) 2013-12-20 2021-12-24 Agenebio Inc Benzodiazepine derivatives, compositions, and methods for treating cognitive impairment
WO2015115673A1 (en) * 2014-01-31 2015-08-06 Ono Pharmaceutical Co., Ltd. Fused imidazole compounds
AR099287A1 (en) * 2014-02-17 2016-07-13 Bayer Cropscience Ag DERIVATIVES OF CONDENSED BICYCLIC HETEROCICLES SUBSTITUTED WITH 2- (HET) ARILO AS AGENTS TO COMBAT PARASITES
JP6785217B2 (en) * 2014-07-31 2020-11-18 インスティチュート パスツール コリア Use as 2-amino-benzimidazole derivatives and their 5-lipoxygenase and / or prostaglandin E synthase inhibitors
EP3954685B1 (en) 2014-12-29 2025-08-27 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Small molecule inhibitors of lactate dehydrogenase and methods of use thereof
UY36547A (en) 2015-02-05 2016-06-01 Bayer Cropscience Ag BICYCLIC CONDENSED HETEROCYCLIC DERIVATIVES REPLACED BY 2- (HET) ARILO AS PESTICIDES
UY36548A (en) 2015-02-05 2016-06-01 Bayer Cropscience Ag BICYCLIC CONDENSED HETEROCYCLIC DERIVATIVES REPLACED BY 2- (HET) ARILO AS PESTICIDES
CN107810188B (en) 2015-04-08 2020-09-22 拜耳作物科学股份公司 Fused bicyclic heterocyclic derivatives as pest control agents and intermediate products
CN113264939A (en) 2015-06-19 2021-08-17 艾吉因生物股份有限公司 Benzodiazepine derivatives, compositions and methods for treating cognitive impairment
CN107922396B (en) 2015-07-20 2022-08-05 建新公司 Colony stimulating factor-1 receptor (CSF-1R) inhibitors
CN118955472A (en) 2015-08-07 2024-11-15 拜耳作物科学股份公司 2-(Hetero)aryl substituted fused heterocyclic derivatives as pesticides
RU2018119344A (en) 2015-10-26 2019-11-28 Байер Кропсайенс Акциенгезельшафт CONDENSED BICYCLIC HETEROCYCLIC DERIVATIVES AS A MEANS FOR COMBATING Pests
WO2017093180A1 (en) 2015-12-01 2017-06-08 Bayer Cropscience Aktiengesellschaft Condensed bicyclic heterocycle derivatives as pest control agents
WO2017144341A1 (en) 2016-02-23 2017-08-31 Bayer Cropscience Aktiengesellschaft Condensed bicyclic heterocycle derivatives as pest control agents
WO2017157332A1 (en) * 2016-03-18 2017-09-21 江苏恒瑞医药股份有限公司 Aromatic amide derivative, preparation method therefor, and pharmaceutical applications thereof
WO2017174414A1 (en) 2016-04-05 2017-10-12 Bayer Cropscience Aktiengesellschaft Naphthaline-derivatives as pest control agents
AR108257A1 (en) 2016-05-02 2018-08-01 Mei Pharma Inc POLYMORPHIC FORMS OF 3- [2-BUTIL-1- (2-DIETILAMINO-ETIL) -1H-BENCIMIDAZOL-5-IL] -N-HYDROXY-ACRYLAMIDE AND USES OF THE SAME
EP3241830A1 (en) 2016-05-04 2017-11-08 Bayer CropScience Aktiengesellschaft Condensed bicyclic heterocyclic derivatives as pesticides
CN109689662B (en) 2016-07-19 2022-03-15 拜耳作物科学股份公司 Fused bicyclic heterocyclic derivatives as pest control agents
BR112019003158B1 (en) 2016-08-15 2022-10-25 Bayer Cropscience Aktiengesellschaft DERIVATIVES OF CONDENSED BICYCLIC HETEROCYCLE, THEIR USE, AGROCHEMICAL FORMULATION, AND METHOD TO CONTROL ANIMAL PESTS
KR102420235B1 (en) 2016-08-16 2022-07-12 바이엘 크롭사이언스 악티엔게젤샤프트 2-(3,6-dihalopyridin-2-yl)-3H-imidazole[4,5-c by reaction of 3H-imidazole[4,5-c]pyridine derivative with organometallic zinc-amine base ]Pyridine derivatives and methods for preparing related compounds
CN109963860A (en) 2016-09-19 2019-07-02 拜耳作物科学股份公司 Pyrazolo [1,5-A ] pyridine derivatives and their use as pesticides
WO2018065292A1 (en) 2016-10-06 2018-04-12 Bayer Cropscience Aktiengesellschaft 2-(het)aryl-substituted condensed bicyclic heterocycle derivatives as pest control agents
US10961248B2 (en) 2016-12-01 2021-03-30 Syngenta Participations Ag Pesticidally active heterocyclic derivatives with sulfur containing substituents
US11505555B2 (en) 2016-12-19 2022-11-22 Agenebio, Inc. Benzodiazepine derivatives, compositions, and methods for treating cognitive impairment
US20180170941A1 (en) 2016-12-19 2018-06-21 Agenebio, Inc. Benzodiazepine derivatives, compositions, and methods for treating cognitive impairment
WO2018138050A1 (en) 2017-01-26 2018-08-02 Bayer Aktiengesellschaft Condensed bicyclic heterocyclene derivatives as pest control agents
TW201833107A (en) 2017-02-06 2018-09-16 德商拜耳廠股份有限公司 2-(het)aryl-substituted fused heterocycle derivatives as pesticides
EP3615540B1 (en) 2017-04-24 2022-04-27 Bayer Aktiengesellschaft Condensed bicyclic heterocycle derivatives as pesticides
USRE50663E1 (en) 2017-10-04 2025-11-18 Bayer Aktiengesellschaft Heterocycle derivatives as pesticides
EP3305786A3 (en) 2018-01-22 2018-07-25 Bayer CropScience Aktiengesellschaft Condensed bicyclic heterocycle derivatives as pesticides
JP2021514949A (en) 2018-02-21 2021-06-17 バイエル・アクチエンゲゼルシヤフト Condensed bicyclic heterocyclic derivative as a pest control agent
AU2019254499A1 (en) 2018-04-20 2020-12-03 Bayer Aktiengesellschaft Heterocyclene derivatives as pest control agents
US11414425B2 (en) 2018-06-19 2022-08-16 Agenebio, Inc. Benzodiazepine derivatives, compositions, and methods for treating cognitive impairment
CN112119065B (en) * 2018-07-13 2024-01-23 四川科伦博泰生物医药股份有限公司 Benzodiazepine compounds, their preparation methods and uses
EP3636645A1 (en) 2018-10-11 2020-04-15 Bayer Aktiengesellschaft Process for the preparation of sulfur-substituted pyridine derivatives
KR20210133240A (en) 2019-02-26 2021-11-05 바이엘 악티엔게젤샤프트 Fused Bicyclic Heterocycle Derivatives as Pesticides
EP3931192B1 (en) 2019-02-26 2024-03-20 Bayer Aktiengesellschaft Condensed bicyclic heterocycle derivatives as pesticides
AR119140A1 (en) 2019-06-13 2021-11-24 Pi Industries Ltd FUSED HETEROCYCLIC COMPOUNDS AND THEIR USE AS PEST CONTROL AGENTS
CN110407744A (en) * 2019-08-13 2019-11-05 上海毕得医药科技有限公司 A kind of synthetic method of 1- (4-aminopyridine -2- base) ethyl ketone
CN111072681B (en) * 2019-12-11 2020-11-06 郑州大学 A kind of fluorescent probe targeting cell membrane surface GABA receptor and its preparation method and application
EP4267573A1 (en) 2020-12-23 2023-11-01 Genzyme Corporation Deuterated colony stimulating factor-1 receptor (csf-1r) inhibitors
US20240132513A1 (en) 2022-08-19 2024-04-25 Agenebio, Inc. Benzazepine derivatives, compositions, and methods for treating cognitive impairment
CN115490646A (en) * 2022-10-10 2022-12-20 安徽昊帆生物有限公司 Preparation method of 3-nitro-1,2,4-triazole
CN117486846B (en) * 2023-10-31 2025-10-03 陕西科技大学 A total synthesis method of kavalactone compounds

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3150486A1 (en) * 1981-12-19 1983-08-25 Merck Patent Gmbh, 6100 Darmstadt IMIDAZO (4,5-C) PYRIDINE, PHARMACEUTICAL PREPARATIONS CONTAINING THE SAME AND METHOD FOR THE PRODUCTION THEREOF
WO1996033194A1 (en) 1995-04-21 1996-10-24 Neurosearch A/S Benzimidazole compounds and their use as modulators of the gaba a receptor complex
GB9702524D0 (en) * 1997-02-07 1997-03-26 Merck Sharp & Dohme Therapeutic agents
CA2282761A1 (en) 1997-03-04 1998-09-11 Medtronic, Inc. Bioprothesis film strip for surgical stapler and method of attaching the same
CA2369553A1 (en) 1999-04-02 2000-10-12 Raymond F. Horvath N-benzimidazolylmethyl and n-indolylmethyl-benzamides and their use as crf modulators
ES2226812T3 (en) * 1999-04-02 2005-04-01 Neurogen Corporation AMINOALQUILIMIDAZOL DERIVATIVES CONDENSED WITH ARILO AND HETEROARILO: SELESCTIVE MODULATORS OF THE GABAA PRESEPTORS.
CA2369549A1 (en) 1999-04-02 2000-10-12 Robert W. Desimone Aryl and heteroaryl fused aminoalkyl-imidazole derivatives and their use as antidiabetics
JP2002541145A (en) 1999-04-02 2002-12-03 ニューロゲン コーポレイション Aryl and heteroaryl fused aminoalkyl-imidazole derivatives: Bradykinin B2 receptor selective modulators
US6512062B1 (en) * 1999-04-08 2003-01-28 The University Of North Carolina At Chapel Hill Polymerization of non-fluorinated monomers in carbon dioxide
AU777961B2 (en) 1999-06-22 2004-11-04 Neurosearch A/S Novel benzimidazole derivatives and pharmaceutical compositions comprising these compounds
GB9921150D0 (en) * 1999-09-07 1999-11-10 Merck Sharp & Dohme Therapeutic agents
WO2002049993A2 (en) 2000-09-29 2002-06-27 Neurogen Corporation High affinity small molecule c5a receptor modulators
US20030055037A1 (en) 2000-10-06 2003-03-20 Delombaert Stephane Benzimidazole and indole derivatives as CRF receptor modulators
US7030150B2 (en) 2001-05-11 2006-04-18 Trimeris, Inc. Benzimidazole compounds and antiviral uses thereof

Also Published As

Publication number Publication date
HRP20030483A2 (en) 2005-04-30
AU3276802A (en) 2002-07-01
AR035774A1 (en) 2004-07-14
AP1503A (en) 2005-12-24
CA2431592A1 (en) 2002-06-27
MXPA03005493A (en) 2004-04-20
TWI304402B (en) 2008-12-21
WO2002050062A3 (en) 2003-01-16
YU51703A (en) 2006-05-25
SK7672003A3 (en) 2004-07-07
NO326558B1 (en) 2009-01-12
UY27095A1 (en) 2002-07-31
AU2002232768B2 (en) 2007-09-06
BG107899A (en) 2004-08-31
ES2256325T3 (en) 2006-07-16
US20030069257A1 (en) 2003-04-10
US20060025425A1 (en) 2006-02-02
CO5390086A1 (en) 2004-04-30
PE20020720A1 (en) 2002-08-12
KR100854174B1 (en) 2008-08-26
PA8535601A1 (en) 2002-11-28
EE200300304A (en) 2003-12-15
MA26974A1 (en) 2004-12-20
EA200300713A1 (en) 2004-02-26
EA007157B1 (en) 2006-08-25
EP1368342B3 (en) 2010-12-01
US7642267B2 (en) 2010-01-05
HRP20030483A9 (en) 2007-11-30
ATE304008T1 (en) 2005-09-15
BR0116385A (en) 2005-12-13
EE05108B1 (en) 2008-12-15
ZA200304544B (en) 2005-05-25
CZ20031676A3 (en) 2004-03-17
CN1553909A (en) 2004-12-08
EP1368342B1 (en) 2005-09-07
AP2003002818A0 (en) 2003-06-30
IS6855A (en) 2003-06-20
KR20030071789A (en) 2003-09-06
US20080227793A1 (en) 2008-09-18
EP1368342A2 (en) 2003-12-10
DE60113302D1 (en) 2005-10-13
IL156294A0 (en) 2004-01-04
HUP0303849A2 (en) 2004-03-01
NO20032834L (en) 2003-08-08
OA12539A (en) 2006-06-05
DE60113302T2 (en) 2006-06-29
HUP0303849A3 (en) 2009-08-28
NZ526330A (en) 2006-09-29
JP2004536782A (en) 2004-12-09
MY141733A (en) 2010-06-15
US7300945B2 (en) 2007-11-27
NO20032834D0 (en) 2003-06-20
PL366243A1 (en) 2005-01-24
ECSP034656A (en) 2003-10-28
EG24590A (en) 2009-12-07
US6916819B2 (en) 2005-07-12
UA75902C2 (en) 2006-06-15
WO2002050062A2 (en) 2002-06-27

Similar Documents

Publication Publication Date Title
US7642267B2 (en) Benzimidazole and pyridylimidazole derivatives
AU2005321946B2 (en) Enzyme modulators and treatments
US6936617B2 (en) Heteroaryl substituted fused bicyclic heteroaryl compound as GABAA receptor ligands
JP6705862B2 (en) Imidazo[4,5-C]pyridine derivatives and pyrrolo[2,3-C]pyridine derivatives as SSAO inhibitors
TWI386406B (en) Novel derivatives of fluorene, compositions containing them and use thereof
US20220081427A1 (en) Inhibitors of jun n-terminal kinase
JP2012514044A (en) Heteroaryl compounds useful as Raf kinase inhibitors
AU2010341573A1 (en) Isoindolinone inhibitors of phosphatidylinositol 3-kinase
JP6285470B2 (en) New compounds
KR101822767B1 (en) Inhibitors of bruton&#39;s tyrosine kinase
CA2484936A1 (en) Substituted imidazolylmethyl pyridine and pyrazine derivatives and their use as gabaa receptor ligands
JP2009512666A (en) 6-Heteroaryl pyridoindolone derivatives, their preparation and therapeutic use
HK1068133A (en) Benzimidazole and pyridylimidazole derivatives as ligands for gaba receptors
HK1169113A (en) Substituted triazole and imidazole derivatives as gamma secretase modulators

Legal Events

Date Code Title Description
STCB Information on status: application discontinuation

Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION