US20100255132A1 - Novel botanical formulation for treating sickle cell disease - Google Patents
Novel botanical formulation for treating sickle cell disease Download PDFInfo
- Publication number
- US20100255132A1 US20100255132A1 US12/798,524 US79852410A US2010255132A1 US 20100255132 A1 US20100255132 A1 US 20100255132A1 US 79852410 A US79852410 A US 79852410A US 2010255132 A1 US2010255132 A1 US 2010255132A1
- Authority
- US
- United States
- Prior art keywords
- sorghum bicolor
- sickle cell
- cell disease
- extract
- composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 22
- 208000007056 sickle cell anemia Diseases 0.000 title claims abstract description 22
- 238000009472 formulation Methods 0.000 title description 8
- 240000006394 Sorghum bicolor Species 0.000 claims abstract description 28
- 239000000284 extract Substances 0.000 claims abstract description 15
- 239000000463 material Substances 0.000 claims abstract description 15
- 241000196324 Embryophyta Species 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 12
- 230000002401 inhibitory effect Effects 0.000 claims abstract 2
- 230000002869 anti-sickling effect Effects 0.000 claims description 13
- 238000000605 extraction Methods 0.000 claims description 9
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 6
- 239000002775 capsule Substances 0.000 claims description 4
- 239000000725 suspension Substances 0.000 claims description 4
- 239000003826 tablet Substances 0.000 claims description 4
- 239000003937 drug carrier Substances 0.000 claims description 3
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 3
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 3
- 239000000243 solution Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 2
- 239000007864 aqueous solution Substances 0.000 claims 1
- 238000001914 filtration Methods 0.000 claims 1
- 238000004108 freeze drying Methods 0.000 claims 1
- 238000002156 mixing Methods 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 5
- 238000002360 preparation method Methods 0.000 abstract description 3
- 235000007230 Sorghum bicolor Nutrition 0.000 description 18
- 210000004027 cell Anatomy 0.000 description 8
- 238000004166 bioassay Methods 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 229940124574 antisickling agent Drugs 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 210000003743 erythrocyte Anatomy 0.000 description 3
- VSNHCAURESNICA-NJFSPNSNSA-N 1-oxidanylurea Chemical compound N[14C](=O)NO VSNHCAURESNICA-NJFSPNSNSA-N 0.000 description 2
- 239000008979 Niprisan Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000003389 potentiating effect Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 208000019838 Blood disease Diseases 0.000 description 1
- 206010065553 Bone marrow failure Diseases 0.000 description 1
- 102100021519 Hemoglobin subunit beta Human genes 0.000 description 1
- 108091005904 Hemoglobin subunit beta Proteins 0.000 description 1
- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 1
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 1
- 108010016797 Sickle Hemoglobin Proteins 0.000 description 1
- 208000000859 Sickle cell trait Diseases 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 229940041181 antineoplastic drug Drugs 0.000 description 1
- 239000003939 antisickling agent Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 239000003974 emollient agent Substances 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 208000014951 hematologic disease Diseases 0.000 description 1
- 208000018706 hematopoietic system disease Diseases 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000005414 inactive ingredient Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229940072033 potash Drugs 0.000 description 1
- 235000015320 potassium carbonate Nutrition 0.000 description 1
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 229940002612 prodrug Drugs 0.000 description 1
- 239000000651 prodrug Substances 0.000 description 1
- 108010006031 sickle deoxyhemoglobin Proteins 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/06—Antianaemics
Definitions
- the present invention relates to the field of botanical formulations, botanical compositions and botanical extracts, particularly as it relates to the phytodrug, prodrug or plant medicament for the treatment and management of sickle cell disease and methods of preparing and using same.
- Sickle cell disease is a hereditary blood disorder, affecting over 75,000 people in the United States. Sickle cell disease (SCD) affects over twenty million people throughout the world and is particularly common among those whose ancestors come from sub-Saharan Africa, Spanish-speaking regions in the Western Hemisphere (South America, the Caribbean, and Central America), Saudi Arabia, India, and Mediterranean countries such as Turkey, Greece, and Italy.
- U.S. Pat. No. 5,800,819 describes a composition requiring extracts from at least four plant materials for treating sickle cell disease.
- U.S. Pat. No. 5,800,819 does not describe Sorghum bicolor, per se, having anti-sickling activity nor measure the anti-sickling activity of Sorghum bicolor. It only describes the anti-sickling activity of the four ingredients mixed together.
- the present invention provides a distinctly different and improved plant formulation using Sorghum bicolor plant material, per se, that shows potent anti-sickling activity against human sickle cells and is simpler to formulate.
- a botanical formulation comprising an extraction obtained from Sorghum bicolor plant, without the use of, or extraction from, any other plant, said extraction from Sorghum bicolor plant being effective against sickle cell disease.
- the Sorghum bicolor plant alone, has not been previously shown to contain anti-sickling activity or anti-sickling compounds.
- the formulation could be administered in any suitable form such as a tablet, capsule, suspension, solution, powder, and the like, to treat sickle cell disease and may further comprise any pharmaceutically acceptable carrier and excipients well known to a skilled artisan in the field to which this invention belongs.
- a method for preparing a botanical composition comprises the steps of:
- Sorghum bicolor seeds were grown under normal green house conditions. Canadian pot mixture fortified with anhydrous ferric chloride (10 mg/pot) was used as the growth medium. Watering was done with tap water once a week. Every month the leaves were removed and air-dried. The dried leaves were extracted with aqueous sodium bicarbonate (7.4% concentration W/V at a pH ranging from about 8 to about 8.3) by stirring for about 8 hrs at normal ambient temperature. It should be noted that other buffers or extractants, neutral, organic or inorganic, at different pH could be employed to extract the anti-sickling activity from Sorghum bicolor. The extraction process is not limited to sodium bicarbonate or the pH range of 8.0-8.3.
- the method described herein is only a preferred embodiment of the extraction process. It should also be noted that the manner in which the plant is grown, including but not limited to the soil type, fertility and soil pH may also vary from the method described herein and would influence the amount of antisickling activity in the extract obtained from the plant material. The degree to which plants could vary in the accumulation of natural products that may provide medicinal, therapeutic and biological activity is well known to skilled artisans and the ability to standardize the extract to ensure pharmacologically efficacious activity by any suitable method is included in this invention, the bioassay described herein being only an example.
- the extract was allowed to settle, and then decanted, filtered, centrifuged and freeze-dried.
- the freeze-dried material was thoroughly blended to form a uniform mixture, which could be formulated in any suitable form, such as tablets, granules, capsules, suspensions, solutions, and the like, by well known methods.
- Buffers, emollients, additives, supplements, preservatives, inactive ingredients, fillers, and the like could be added to any formulation prepared by using the Sorghum bicolor extract.
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Medical Informatics (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Diabetes (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
An improved plant medicament composition, comprising an extract of Sorghum bicolor plant material for treating sickle cell disease is described. A method for the preparation of said composition having the property of inhibiting sickle cells is also provided.
Description
- The present invention relates to the field of botanical formulations, botanical compositions and botanical extracts, particularly as it relates to the phytodrug, prodrug or plant medicament for the treatment and management of sickle cell disease and methods of preparing and using same.
- Sickle cell disease (SCD) is a hereditary blood disorder, affecting over 75,000 people in the United States. Sickle cell disease (SCD) affects over twenty million people throughout the world and is particularly common among those whose ancestors come from sub-Saharan Africa, Spanish-speaking regions in the Western Hemisphere (South America, the Caribbean, and Central America), Saudi Arabia, India, and Mediterranean countries such as Turkey, Greece, and Italy.
- In the US, those with SCD have an average mortality in their 40s, a poor quality of life and high medical costs. In SCD, a mutation in β-globin (glu6val) causes deoxygenated sickle hemoglobin (deoxy-HbS) to form insoluble polymers inside red blood cells (RBCs), which deforms the RBCs into rigid shapes or sickle cells that occlude capillaries and small blood vessels. The only disease-modifying drug approved for use in SCD patients is hydroxyurea, an anti-cancer drug. Not all patients respond to hydroxyurea, and it can be poorly tolerated causing myelosuppression in some patients. Despite extensive studies on SCD by researchers over several decades, there has been little progress in the development of additional disease modifying agents. Therefore, new, safer and more effective therapeutic anti-sickling agents are needed to treat patients with SCD, particularly children, which could improve the quality of life, increase the life expectancies of sufferers of this disease, and reduce the estimated 100,000 hospitalizations and $500 million in direct hospital costs due to sickle cell disease in the United States. Sickle cell disease occurs in about 1 in every 500 African American births, and about 1 in 12 African Americans has sickle cell trait. The morbidity and mortality factors associated with sickle cell disease are well known and the acute and chronic trauma of the painful episodes is indescribable. In view of these realities, there is a desperate need for drugs or agents that could alleviate and mitigate the effects of this terrible disease.
- U.S. Pat. No. 5,800,819 describes a composition requiring extracts from at least four plant materials for treating sickle cell disease. U.S. Pat. No. 5,800,819 does not describe Sorghum bicolor, per se, having anti-sickling activity nor measure the anti-sickling activity of Sorghum bicolor. It only describes the anti-sickling activity of the four ingredients mixed together. The present invention provides a distinctly different and improved plant formulation using Sorghum bicolor plant material, per se, that shows potent anti-sickling activity against human sickle cells and is simpler to formulate.
- It is an object of this invention to provide an improved and efficacious botanical medicament for the treatment and management of sickle cell disease.
- It is an additional object of this invention to provide a method for the preparation of an improved plant formulation and preparation for the treatment and management of sickle cell disease.
- Various other objects and advantages of the invention will become obvious from the detailed description of the invention.
- Various objects and advantages of the invention are achieved by preparing a botanical formulation comprising an extraction obtained from Sorghum bicolor plant, without the use of, or extraction from, any other plant, said extraction from Sorghum bicolor plant being effective against sickle cell disease. The Sorghum bicolor plant, alone, has not been previously shown to contain anti-sickling activity or anti-sickling compounds. The formulation could be administered in any suitable form such as a tablet, capsule, suspension, solution, powder, and the like, to treat sickle cell disease and may further comprise any pharmaceutically acceptable carrier and excipients well known to a skilled artisan in the field to which this invention belongs.
- A method for preparing a botanical composition, comprises the steps of:
-
- (i) obtaining an extract having anti-sickling activity from Sorghum bicolor plant material; and
- (ii) preparing said extract in a suitable form for treating sickle cells.
- Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention belongs. The term “pharmaceutically acceptable,” as used herein, refers to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of patients without excessive toxicity, irritation, allergic response, or other problem or complication commensurate with a reasonable benefit/risk ratio, and are effective for the intended use.
- A typical procedure for extraction using Sorghum bicolor plant material is now set forth. Sorghum bicolor seeds were grown under normal green house conditions. Canadian pot mixture fortified with anhydrous ferric chloride (10 mg/pot) was used as the growth medium. Watering was done with tap water once a week. Every month the leaves were removed and air-dried. The dried leaves were extracted with aqueous sodium bicarbonate (7.4% concentration W/V at a pH ranging from about 8 to about 8.3) by stirring for about 8 hrs at normal ambient temperature. It should be noted that other buffers or extractants, neutral, organic or inorganic, at different pH could be employed to extract the anti-sickling activity from Sorghum bicolor. The extraction process is not limited to sodium bicarbonate or the pH range of 8.0-8.3. The method described herein is only a preferred embodiment of the extraction process. It should also be noted that the manner in which the plant is grown, including but not limited to the soil type, fertility and soil pH may also vary from the method described herein and would influence the amount of antisickling activity in the extract obtained from the plant material. The degree to which plants could vary in the accumulation of natural products that may provide medicinal, therapeutic and biological activity is well known to skilled artisans and the ability to standardize the extract to ensure pharmacologically efficacious activity by any suitable method is included in this invention, the bioassay described herein being only an example.
- The extract was allowed to settle, and then decanted, filtered, centrifuged and freeze-dried.
- The freeze-dried material was thoroughly blended to form a uniform mixture, which could be formulated in any suitable form, such as tablets, granules, capsules, suspensions, solutions, and the like, by well known methods. Buffers, emollients, additives, supplements, preservatives, inactive ingredients, fillers, and the like, well known to one of ordinary skill in the art to which this invention belongs, could be added to any formulation prepared by using the Sorghum bicolor extract.
- Chemical analyses of extracts from several Sorghum bicolor varieties obtained from various sources were carried out using HPLC. The sickle cell bioassay was carried out by using blood removed from a sickle cell subject and exposed to the extract in vitro to measure antisickling activity employing counting and imaging techniques as described in Iyamu, et al, British Journal of Hematology, (2002), 118: 337-343.
- The results shown in Table 1 indicate that Sorghum bicolor extract, without using plant material from any other source, has potent antisickling activity, having as much as five-fold greater anti-sickling efficacy against human sickle cells, compared to the prior art formulation disclosed in U.S. Pat. No. 5,800,819.
- It should be noted that the guidance, illustrations and examples provided herein are only illustrative and not limiting, and various alternate embodiments, modifications or manipulations of the present invention would be suggested to a skilled artisan and these are included within the spirit and purview of this application and scope of the appended claims.
-
TABLE 1 COMPARATIVE RESULTS OF SICKLE CELL BIOASSAY Solvent used for Origin of Bio-Assay concentration Sorghum (% of cells still Name of sample Sample ID at pH 8-8.3 Bicolor seed Sample description sickled) Sorghum Bicolor XC-214-41-IS2724 Sod. Bi Carb India IS2724 1 month old leaves 23% Sorghum Bicolor IS2724-4L Sod. Bi Carb India IS2724 4 month old leaves 38% Sorghum Bicolor IS2724-ML-5 Sod. Bi Carb India IS2724 5 month old leaves 32% Sorghum Bicolor IS2724-IF-4 Sod. Bi Carb India IS2724 inflorescent leaves 16% Sorghum Bicolor IS2724-SD-5 Sod. Bi Carb India IS2724 ground seeds 62% Sorghum Bicolor IS2724-ST-5 Sod. Bi Carb India IS2724 stem 63% Sorghum Bicolor XC-214-41-FF Sod. Bi Carb India Fara Fara 1 month old leaves 36% Sorghum Bicolor XC-214-41-Red Sod. Bi Carb India Red Hagari 1 month old leaves 23% Sorghum Bicolor PI48771-02-SD Sod. Bi Carb South Africa 1 month old green leaves 52% Sorghum Bicolor XC-214--38-sb4g Sod. Bi Carb South Africa 4 month old green leaves 32% Sorghum Bicolor XC-214-42-SA6 Sod. Bi Carb South Africa 6 month old green leaves 24% Sorghum Bicolor PI586787-01-SD Sod. Bi Carb Unknown Origin 4 month old green leaves 42% Sorghum Bicolor PI513794-01-SD Sod. Bi Carb Niger 4 month old green leaves 60% Sorghum Bicolor PI155889-0 1-SD Sod. Bi Carb Tanzania 4 month old green leaves 64% Sorghum Bicolor PI287617-01-SD Sod. Bi Carb Zimbabwe 4 month old green leaves 53% Sorghum Bicolor PI28497I-01-SD Sod. Bi Carb Argentina 4 month old green leaves 51% Sorghum Bicolor PI810693-01-SD Sod. Bi Carb China 4 month old green leaves 54% Sorghum Bicolor PI585454-01-SD Sod. Bi Carb Ghana 4 month old green leaves 28% Sorghum Bicolor XC-214-41-Nunba Sod. Bi Carb Nigeria Nunaba, 1 month old leaves 24% Sorghum Bicolor XC-214-41-Nig2 Sod. Bi Carb Nigeria 2 month old green leaves 16% Sorghum Bicolor XC-214-41-Nig6 Sod. Bi Carb Nigeria 6 month old green leaves 26% Nicosan U.S. Pat. No. 5,800,819 Potash Nigeria Nicosan from Nigeria 88% Control-Buffer Ctr 500 0 97% Control-Buffer Ctr 500 0 98%
Claims (7)
1. A botanical composition for inhibiting sickling in sickle cell disease, comprising an extraction from Sorghum bicolor plant material, without using material from any other plant, said extraction being effective against sickle cells.
2. The composition of claim 1 further comprising any pharmaceutically acceptable carrier and excipients.
3. The composition of claim 2 , wherein said extraction is formulated as a solution, suspension, powder, tablet or capsule.
4. A method for preparing a botanical composition, comprising the steps of:
(i) obtaining an extract having anti-sickling activity from Sorgum bicolor plant material; and
(ii) preparing said extract in a suitable form for treating sickle cells.
5. The method of claim 4 , comprising the steps of:
(i) extracting Sorghum bicolor plant material with a 7.4% weight by volume aqueous solution of sodium bicarbonate at a pH ranging from about 8.0 to about 8.3 by stirring for about 8 hr at normal ambient temperature;
(ii) allowing the extract to settle, then decanting, filtering, centrifuging and freeze drying the resulting material; and
(iii) thoroughly blending the freeze dried material to form a uniform mixture.
6. The method of claim 5 , further comprising any pharmaceutically acceptable carrier and excipients.
7. The method of claim 6 , administering said blended uniform mixture for treating sickle cell disease in a powder, tablet, capsule, granular or suspension form.
Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/798,524 US20100255132A1 (en) | 2009-04-06 | 2010-04-06 | Novel botanical formulation for treating sickle cell disease |
| PCT/US2011/031051 WO2011126965A1 (en) | 2010-04-06 | 2011-04-04 | A novel botanical formulation for treating sickle cell disease |
| ES11714476.6T ES2655696T3 (en) | 2010-04-06 | 2011-04-04 | A new formulation with plant ingredients for the treatment of sickle cell disease |
| EP11714476.6A EP2555786B1 (en) | 2010-04-06 | 2011-04-04 | A novel botanical formulation for treating sickle cell disease |
| BR112012025540-1A BR112012025540B1 (en) | 2010-04-06 | 2011-04-04 | Use of a composition comprising an extract of bicolor sorghum plant material for the manufacture of a medicament for the treatment of sickle cell disease and method for preparing said composition |
| US15/899,077 US10471116B2 (en) | 2010-04-06 | 2018-02-19 | Botanical formulation for treating sickle cell disease |
| US16/597,527 US11590195B2 (en) | 2010-04-06 | 2019-10-09 | Botanical formulation for treating sickle cell disease |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US21201709P | 2009-04-06 | 2009-04-06 | |
| US12/798,524 US20100255132A1 (en) | 2009-04-06 | 2010-04-06 | Novel botanical formulation for treating sickle cell disease |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US15/899,077 Continuation US10471116B2 (en) | 2010-04-06 | 2018-02-19 | Botanical formulation for treating sickle cell disease |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| US20100255132A1 true US20100255132A1 (en) | 2010-10-07 |
Family
ID=43901643
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US12/798,524 Abandoned US20100255132A1 (en) | 2009-04-06 | 2010-04-06 | Novel botanical formulation for treating sickle cell disease |
| US15/899,077 Active US10471116B2 (en) | 2010-04-06 | 2018-02-19 | Botanical formulation for treating sickle cell disease |
| US16/597,527 Active 2030-06-14 US11590195B2 (en) | 2010-04-06 | 2019-10-09 | Botanical formulation for treating sickle cell disease |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US15/899,077 Active US10471116B2 (en) | 2010-04-06 | 2018-02-19 | Botanical formulation for treating sickle cell disease |
| US16/597,527 Active 2030-06-14 US11590195B2 (en) | 2010-04-06 | 2019-10-09 | Botanical formulation for treating sickle cell disease |
Country Status (5)
| Country | Link |
|---|---|
| US (3) | US20100255132A1 (en) |
| EP (1) | EP2555786B1 (en) |
| BR (1) | BR112012025540B1 (en) |
| ES (1) | ES2655696T3 (en) |
| WO (1) | WO2011126965A1 (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5225447A (en) * | 1988-03-28 | 1993-07-06 | Omex International, Inc. | Composition and method for the treatment of sickle cell anemia |
| US5800819A (en) * | 1996-01-25 | 1998-09-01 | National Institute For Pharmaceutical Research And Development Federal Ministry Of Science And Technology | Piper guineense, pterocarpus osun, eugenia caryophyllata, and sorghum bicolor extracts for treating sickle cell disease |
| US6669979B1 (en) * | 2000-01-11 | 2003-12-30 | Jifu Zhao | Method for promoting extraction of beneficial compounds into beverages naturally containing same, and for extending the duration of freshness of coffee |
-
2010
- 2010-04-06 US US12/798,524 patent/US20100255132A1/en not_active Abandoned
-
2011
- 2011-04-04 BR BR112012025540-1A patent/BR112012025540B1/en active IP Right Grant
- 2011-04-04 EP EP11714476.6A patent/EP2555786B1/en active Active
- 2011-04-04 WO PCT/US2011/031051 patent/WO2011126965A1/en not_active Ceased
- 2011-04-04 ES ES11714476.6T patent/ES2655696T3/en active Active
-
2018
- 2018-02-19 US US15/899,077 patent/US10471116B2/en active Active
-
2019
- 2019-10-09 US US16/597,527 patent/US11590195B2/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5225447A (en) * | 1988-03-28 | 1993-07-06 | Omex International, Inc. | Composition and method for the treatment of sickle cell anemia |
| US5800819A (en) * | 1996-01-25 | 1998-09-01 | National Institute For Pharmaceutical Research And Development Federal Ministry Of Science And Technology | Piper guineense, pterocarpus osun, eugenia caryophyllata, and sorghum bicolor extracts for treating sickle cell disease |
| US6669979B1 (en) * | 2000-01-11 | 2003-12-30 | Jifu Zhao | Method for promoting extraction of beneficial compounds into beverages naturally containing same, and for extending the duration of freshness of coffee |
Non-Patent Citations (4)
| Title |
|---|
| Iyamu et al., In vitro effects of NIPRISAN (Nix-0699): a naturallyoccurring, potent antisickling agent, 2002, British J Haematology, 118, 337-343 * |
| Nwinyi et al., Evaluation of toxicity profile of leaf base extract ofSorghum bicolor in rat, Jan. 2009, African J Biotechnology, 8: 334-342 * |
| Oladiji et al., 2007, Anti-anaemic potentials of aqueous extract of Sorghum bicolor (L.) moench stem bark in rats, J Enthnopharmacology, 111: 651-656 * |
| Whelan et al., Enzymatic Fractionation of Carbon Isotopes by Phosphoenolypyruvate Carboxylase from C4 Plants, 1973, Plant Physiol, 51: 1051-1054 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2011126965A1 (en) | 2011-10-13 |
| ES2655696T3 (en) | 2018-02-21 |
| EP2555786A1 (en) | 2013-02-13 |
| US20200108113A1 (en) | 2020-04-09 |
| US11590195B2 (en) | 2023-02-28 |
| US10471116B2 (en) | 2019-11-12 |
| BR112012025540B1 (en) | 2021-08-31 |
| BR112012025540A2 (en) | 2016-06-28 |
| EP2555786B1 (en) | 2017-11-01 |
| US20180169174A1 (en) | 2018-06-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Soni et al. | Pharmacological properties of Datura stramonium L. as a potential medicinal tree: an overview | |
| US9149500B2 (en) | Method and process for relieving or preventing symptoms | |
| Mitra et al. | Phytopharmacology of Berberis aristata DC: A review | |
| KR101271054B1 (en) | Pharmaceutical composition for preventing and treating diseases related to renal failure containing extract from Salvia miltiorrhiza radix, Atractylodis macrocephalae rhizome, Polyporus, Poria, Pinelliae rhizome, Coptis rhizome and Rhei radix et rhizoma | |
| CN102218049B (en) | Anti-aging medicine or health food composition and application thereof | |
| CN110623998B (en) | Traditional Chinese medicine composition for diabetic peripheral neuropathy and application thereof | |
| US11590195B2 (en) | Botanical formulation for treating sickle cell disease | |
| CN111905017A (en) | A kind of Chinese medicine composition and application of anti-Helicobacter pylori | |
| KR102095536B1 (en) | Oral formulation for improved dissolution rate and disintegrability of herbal extract | |
| CN101254217A (en) | A preparation method of the extract of the renewable part of the yew tree and the application of the extract in the preparation of oral anticancer drugs | |
| US7250180B2 (en) | Anti-prostate cancer composition and therapeutic uses therefor | |
| RS54162B1 (en) | PHARMACEUTICAL COMPOSITION BASED ON MEDICINAL HERBS FOR HUMAN AND VETERINARY MEDICINAL PRODUCTS | |
| US20110300242A1 (en) | Novel botanical formulation for treating sickle cell disease | |
| RU2331430C1 (en) | Medicinal herbal mix for stomach ulcer prevention and complex treatment | |
| CN111346089A (en) | Medicinal preparation and preparation method thereof | |
| WO2006030426A1 (en) | Herbal compositions for treatment of diabetes | |
| Ekoja et al. | Medicinal properties of Ricinus communis and the need for novel formulation of the extracts: A review | |
| CN115779007A (en) | Application of raspberry extract in the preparation of anti-helicobacter pylori products | |
| RU2259205C1 (en) | Curative-prophylactic agent in disease of urogenital system in men | |
| WO2017020861A1 (en) | Application of polygonum capitatum composition in resisting helicobacter pylori | |
| US20200000847A1 (en) | Pharmaceutical composition for treating leukemia and method for preparing the same | |
| KR102039623B1 (en) | Milk thistle granule composition for protecting hepatocyte and manufacturing method thereof | |
| KR101368063B1 (en) | Pharmaceutical composition for preventing and treatmenting containing of Salvia miltiorrhiza from disease related to renal failure | |
| US20120195970A1 (en) | Homeopathic Formulations For Treatment Of Herpes Virus Symptoms | |
| AU716895B2 (en) | Formulation for iron chelation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| AS | Assignment |
Owner name: SWIFT, ROBERT, COLORADO Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:NATHAN, SWAMI;REEL/FRAME:031160/0403 Effective date: 20130828 |
|
| STCB | Information on status: application discontinuation |
Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION |