US20090181994A1

US20090181994A1 - Triazole derivatives as inhibitors of 11-beta-hydroxysteroid dehydrogenase-1

Info

Publication number: US20090181994A1
Application number: US12/364,902
Authority: US
Inventors: Sherman T. Waddell; Gina M. Santorelli; Milana M. Maletic; Aaron H. Leeman; Xin Gu; Donald W. Graham; James M. Balkovec; Susan D. Aster
Original assignee: Merck and Co Inc
Current assignee: Merck Sharp and Dohme LLC
Priority date: 2002-12-20
Filing date: 2009-02-03
Publication date: 2009-07-16
Also published as: JP2006513266A; PE20040936A1; CA2510540A1; PL377183A1; KR20050085790A; HK1087709A1; ATE375336T1; AU2003302255A8; CY1107831T1; DE60316841T2; WO2004058730A3; WO2004058730A2; NZ540465A; BR0317451A; ES2293099T3; IS7876A; ECSP055860A; CL2003002679A1; AR042469A1; CA2510540C

Abstract

Triazole derivatives of structural formula I are selective inhibitors of the 11β-hydroxysteroid dehydrogenase-1. The compounds are useful for the treatment of diabetes, such as noninsulin-dependent diabetes (NIDDM), hyperglycemia, obesity, insulin resistance, dyslipidemia, hyperlipidemia, hypertension, Metabolic Syndrome, and other symptoms associated with NIDDM.

Description

FIELD OF THE INVENTION

The present invention relates to triazole derivatives as inhibitors of the enzyme 11-beta-hydroxysteroid dehydrogenase Type I (11β-HSD-1 or HSD-1) and methods of treatment certain conditions using such compounds. The compounds of the present invention are useful for the treatment of diabetes, such as non-insulin dependent Type 2 diabetes mellitus (NIDDM), insulin resistance, obesity, lipid disorders, hypertension, and other diseases and conditions.

BACKGROUND OF THE INVENTION

Diabetes is caused by multiple factors and is most simply characterized by elevated levels of plasma glucose (hyperglycemia) in the fasting state. There are two generally recognized forms of diabetes: Type 1 diabetes, or insulin-dependent diabetes mellitus (IDDM), in which patients produce little or no insulin, the hormone which regulates glucose utilization, and Type 2 diabetes, or noninsulin-dependent diabetes mellitus (NIDDM), wherein patients produce insulin and even exhibit hyperinsulinemia (plasma insulin levels that are the same or even elevated in comparison with non-diabetic subjects), while at the same time demonstrating hyperglycemia. Type 1 diabetes is typically treated with exogenous insulin administered via injection. However, Type 2 diabetics often develop “insulin resistance”, such that the effect of insulin in stimulating glucose and lipid metabolism in the main insulin-sensitive tissues, namely, muscle, liver and adipose tissues, is diminished. Patients who are insulin resistant but not diabetic have elevated insulin levels that compensate for their insulin resistance, so that serum glucose levels are not elevated. In patients with NIDDM, the plasma insulin levels, even when they are elevated, are insufficient to overcome the pronounced insulin resistance, resulting in hyperglycemia.
Insulin resistance is primarily due to a receptor binding defect that is not yet completely understood. Resistance to insulin results in insufficient activation of glucose uptake, diminished oxidation of glucose and storage of glycogen in muscle, inadequate insulin repression of lipolysis in adipose tissue and inadequate glucose production and secretion by the liver.
Persistent or uncontrolled hyperglycemia that occurs in diabetics is associated with increased morbidity and premature mortality. Abnormal glucose homeostasis is also associated both directly and indirectly with obesity, hypertension and alterations in lipid, lipoprotein and apolipoprotein metabolism. Type 2 diabetics are at increased risk of developing cardiovascular complications, e.g., atherosclerosis, coronary heart disease, stroke, peripheral vascular disease, hypertension, nephropathy, neuropathy and retinopathy. Therefore, therapeutic control of glucose homeostasis, lipid metabolism, obesity and hypertension are critically important in the clinical management and treatment of diabetes mellitus.
Many patients who have insulin resistance but have not developed Type 2 diabetes are also at a risk of developing symptoms referred to as “Syndrome X” or “Metabolic Syndrome”. Syndrome X or Metabolic Syndrome is characterized by insulin resistance, along with abdominal obesity, hyperinsulinemia, high blood pressure, low HDL and high VLDL. These patients, whether or not they develop overt diabetes mellitus, are at increased risk of developing the cardiovascular complications listed above.
Treatment of Type 2 diabetes typically includes physical exercise and dieting. Increasing the plasma level of insulin by administration of sulfonylureas (e.g. tolbutamide and glipizide) or meglitinide, which stimulate the pancreatic β-cells to secrete more insulin, and/or by injection of insulin when sulfonylureas or meglitinide become ineffective, can result in insulin concentrations high enough to stimulate insulin-resistant tissues. However, dangerously low levels of plasma glucose can result, and an increased level of insulin resistance can ultimately occur.
Biguanides increase insulin sensitivity, resulting in some correction of hyperglycemia. However, many biguanides, e.g., phenformin and metformin, cause lactic acidosis, nausea and diarrhea.
The glitazones (i.e. 5-benzylthiazolidine-2,4-diones) form a newer class of compounds with the potential for ameliorating hyperglycemia and other symptoms of Type 2 diabetes. These agents substantially increase insulin sensitivity in muscle, liver and adipose tissue, resulting in partial or complete correction of the elevated plasma levels of glucose substantially without causing hypoglycemia. The glitazones that are currently marketed are agonists of the peroxisome proliferator activated receptor (PPAR) gamma subtype. PPAR-gamma agonism is generally believed to be responsible for the improved insulin sensitization that is observed with the glitazones. Newer PPAR agonists that are being developed for treatment of Type 2 diabetes and/or dyslipidemia are agonists of one or more of the PPAR alpha, gamma and delta subtypes. For a review of insulin-sensitizing agents and other mechanisms for the treatment of Type 2 diabetes, see M. Tadayyon and S. A. Smith, “Insulin sensitisation in the treatment of Type 2 diabetes,” Expert Opin. Investig. Drugs, 12: 307-324 (2003).
There is a continuing need for new methods of treating diabetes and related conditions, such as Metabolic Syndrome. The present invention meets this and other needs.

SUMMARY OF THE INVENTION

The present invention relates to bicyclo[2.2.2]-oct-1-yl-1,2,4-triazoles of structural formula I
These bicyclo[2.2.2]-octyltriazole derivatives are effective as inhibitors of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1). They are therefore useful for the treatment, control or prevention of disorders responsive to the inhibition of 11α-HSD1, such as Type 2 diabetes, lipid disorders, obesity, atherosclerosis, and Metabolic Syndrome.
The present invention also relates to pharmaceutical compositions comprising the compounds of the present invention and a pharmaceutically acceptable carrier.
The present invention also relates to methods for the treatment, control, or prevention of disorders, diseases, or conditions responsive to inhibition of 11β-HSD1 in a subject in need thereof by administering the compounds and pharmaceutical compositions of the present invention.
The present invention also relates to methods for the treatment or control of Type 2 diabetes, obesity, lipid disorders, atherosclerosis, and Metabolic Syndrome by administering the compounds and pharmaceutical compositions of the present invention.
The present invention also relates to methods for treating obesity by administering the compounds of the present invention in combination with a therapeutically effective amount of another agent known to be useful to treat the condition.
The present invention also relates to methods for treating Type 2 diabetes by administering the compounds of the present invention in combination with a therapeutically effective amount of another agent known to be useful to treat the condition.
The present invention also relates to methods for treating atherosclerosis by administering the compounds of the present invention in combination with a therapeutically effective amount of another agent known to be useful to treat the condition.
The present invention also relates to methods for treating lipid disorders by administering the compounds of the present invention in combination with a therapeutically effective amount of another agent known to be useful to treat the condition.
The present invention also relates to methods for treating Metabolic Syndrome by administering the compounds of the present invention in combination with a therapeutically effective amount of another agent known to be useful to treat the condition.
The present invention is also concerned with the use of the compounds of structural formula I for the treatment hyperglycemia, insulin resistance, Type 2 diabetes, lipid disorders, obesity, atherosclerosis, and Metabolic Syndrome.
The present invention also provides for the use of the compounds of structural formula I in the manufacture of a medicament for use in the treatment of hyperglycemia, insulin resistance, Type 2 diabetes, lipid disorders, obesity, atherosclerosis, and Metabolic Syndrome.

DETAILED DESCRIPTION OF THE INVENTION

The present invention is concerned with bicyclo[2.2.2]-oct-1-yl-1,2,4-triazole derivatives useful as inhibitors of 11β-HSD1. Compounds of the present invention are described by structural formula I:
or a pharmaceutically acceptable salt thereof; wherein
each p is independently 0, 1, or 2;
each n is independently 0, 1, or 2;
X is selected from the group consisting of a single bond, O, S(O)_p, NR⁶,
R¹is selected from the group consisting of
arylcarbonyl,
(CH₂)_n-aryl, and
(CH₂)_n-heteroaryl;
in which aryl and heteroaryl are unsubstituted or substituted with one to three substituents independently selected from R⁵;
R²is selected from the group consisting of
hydrogen,
C_1-8alkyl,
C_2-6alkenyl, and
(CH₂)_n—C_3-6cycloalkyl,
in which alkyl, alkenyl, and cycloalkyl are unsubstituted or substituted with one to three substituents independently selected from R⁸and oxo;
each R⁴is independently selected from the group consisting of
hydrogen,
halogen,
hydroxy,
oxo,
C_1-3alkyl, and
C_1-3alkoxy;
R³is selected from the group consisting of
hydrogen,
C_1-10alkyl,
C_2-10alkenyl,
(CH₂)_n—C_3-6cycloalkyl,
(CH₂)_n-aryl,
(CH₂)_n-heteroaryl, and
(CH₂)_n-heterocyclyl;
in which aryl, heteroaryl, and heterocyclyl are unsubstituted or substituted with one to three substituents independently selected from R⁵; and alkyl, alkenyl, and cycloalkyl are unsubstituted or substituted with one to five groups independently selected from R⁸and oxo;
R⁵and R⁸are each independently selected from the group consisting of
hydrogen,
formyl,
C_1-6alkyl,
(CH₂)_n-aryl,
(CH₂)_n-heteroaryl,
(CH₂)_n-heterocyclyl,
(CH₂)_nC_3-7cycloalkyl,
halogen,
OR⁷,
(CH₂)_nN(R⁷)₂,
cyano,
(CH₂)_nCO₂R⁷,
NO₂,
(CH₂)_nNR⁷SO₂R⁶,
(CH₂)_nSO₂N(R⁷)₂,
(CH₂)_nS(O)_pR⁶,
(CH₂)_nSO₂₀R⁷,
(CH₂)_nNR⁷C(O)N(R⁷)₂,
(CH₂)_nC(O)N(R⁷)₂,
(CH₂)_nNR⁶C(O)R⁶,
(CH₂)_nNR⁶CO₂R⁷,
O(CH₂)_nC(O)N(R⁷)₂,
CF₃,
CH₂CF₃,
OCF₃,
OCHCF₂, and
OCH₂CF₃;
wherein aryl, heteroaryl, cycloalkyl, and heterocyclyl are unsubstituted or substituted with one to three substituents independently selected from halogen, hydroxy, C_1-4alkyl, trifluoromethyl, trifluoromethoxy, and C_1-4alkoxy; and wherein any methylene (CH₂) carbon atom in R⁵and R⁸is unsubstituted or substituted with one to two groups independently selected from halogen, hydroxy, and C_1-4alkyl; or two substituents when on the same methylene (CH₂) carbon atom are taken together with the carbon atom to which they are attached to form a cyclopropyl group;
each R⁶is independently selected from the group consisting of
C_1-8alkyl,
(CH₂)_n-aryl,
(CH₂)_n-heteroaryl, and
(CH₂)_nC_3-7cycloalkyl;
wherein alkyl and cycloalkyl are unsubstituted or substituted with one to five substituents independently selected from halogen, oxo, C_1-4alkoxy, C_1-4alkylthio, hydroxy, amino; and aryl and heteroaryl are unsubstituted or substituted with one to three substituents independently selected from cyano, halogen, hydroxy, amino, carboxy, trifluoromethyl, trifluoromethoxy, C_1-4alkyl, and C_1-4alkoxy; or two R⁶groups together with the atom to which they are attached form a 5- to 8-membered mono- or bicyclic ring system optionally containing an additional heteroatom selected from O, S, and NC_1-4alkyl; and
each R⁷is hydrogen or R⁶.
In one embodiment of the compounds of the present invention, R²is cyclopropyl, C_1-3alkyl, or C_2-3alkenyl and R¹is phenyl or naphthyl in which phenyl and naphthyl are unsubstituted or substituted with one to three substituents independently selected from R⁵. In a class of this embodiment, R⁵is selected from the group consisting of halogen, hydroxy, trifluoromethyl, trifluoromethoxy, C_1-3alkyl, C_1-3alkoxy, C_1-3alkylthio, and C_1-3alkylsulfonyl. In a subclass of this class, R²is methyl and R⁴is hydrogen.
In a second embodiment of the compounds of the present invention,
X is a single bond;
R¹is phenyl or naphthyl in which phenyl and naphthyl are unsubstituted or substituted with one to three substituents independently selected from R⁵;
R²is cyclopropyl, C_1-3alkyl, or C_2-3alkenyl; and
R³is C_1-6alkyl unsubstituted or substituted with one to three substituents independently selected from R⁸and oxo.
In a class of this second embodiment, R⁵is selected from the group consisting of halogen, hydroxy, trifluoromethyl, trifluoromethoxy, C_1-3alkyl, C_1-3alkoxy, C_1-3alkylthio, and C_1-3alkylsulfonyl. In a subclass of this class, R²is methyl and R⁴is hydrogen. In another class of this embodiment, R⁸is selected from the group consisting of halogen, hydroxy, oxo, C_1-4alkoxy, C_1-4alkylthio, C_1-4alkylsulfinyl, C_1-4alkylsulfonyl, and phenyl unsubstituted or substituted with one to three groups independently selected from halogen and trifluoromethyl. In a subclass of this class, R²is methyl and R⁴is hydrogen. In a third class of this embodiment, R⁵is selected from the group consisting of halogen, hydroxy, trifluoromethyl, trifluoromethoxy, C_1-3alkyl, C_1-3alkoxy, C_1-3alkylthio, and C_1-3alkylsulfonyl; and R⁸is selected from the group consisting of halogen, hydroxy, oxo, C_1-4alkoxy, C_1-4alkylthio, C_1-4alkylsulfonyl, and phenyl unsubstituted or substituted with one to three groups independently selected from halogen and trifluoromethyl. In a subclass of this class, R²is methyl and R⁴is hydrogen.
In a third embodiment of the compounds of the present invention,
X is a single bond;
R¹is phenyl or naphthyl in which phenyl and naphthyl are unsubstituted or substituted with one to three substituents independently selected from R⁵;
R²is cyclopropyl, C_1-3alkyl, or C_2-3alkenyl; and
R³is phenyl or heteroaryl wherein phenyl and heteroaryl are unsubstituted or substituted with one with one to three substituents independently selected from R⁵.
In a class of this embodiment, R²is methyl and R⁴is hydrogen.
In another class of this embodiment, R³is phenyl unsubstituted or substituted with one with one to three substituents independently selected from R⁵. In a subclass of this class, R⁵is selected from the group consisting of halogen, hydroxy, trifluoromethyl, trifluoromethoxy, C_1-3alkyl, C_1-3alkoxy, C_1-3alkylthio, and C_1-3alkylsulfonyl. In a subclass of this subclass, R²is methyl and R⁴is hydrogen.
In a third class of this embodiment, R³is oxadiazolyl, unsubstituted or substituted with one with one to two substituents independently selected from R⁵. In a subclass of this class, R⁵is phenyl unsubstituted or substituted with one to three substituents independently selected from halogen, hydroxy, C_1-4alkyl, trifluoromethyl, trifluoromethoxy, and C_1-4alkoxy. In a subclass of this subclass, R²is methyl and R⁴is hydrogen.
Illustrative, but nonlimiting examples, of compounds of the present invention that are useful as inhibitors of 11-beta-hydroxysteroid dehydrogenase Type I are the following:
or a pharmaceutically acceptable salt thereof.
As used herein the following definitions are applicable.
“Alkyl”, as well as other groups having the prefix “alk”, such as alkoxy and alkanoyl, means carbon chains which may be linear or branched, and combinations thereof, unless the carbon chain is defined otherwise. Examples of alkyl groups include methyl, ethyl, propyl, isopropyl, butyl, sec- and tert-butyl, pentyl, hexyl, heptyl, octyl, nonyl, and the like. Where the specified number of carbon atoms permits, e.g., from C_3-10, the term alkyl also includes cycloalkyl groups, and combinations of linear or branched alkyl chains combined with cycloalkyl structures. When no number of carbon atoms is specified, C_1-6is intended.
“Alkenyl” means carbon chains which contain at least one carbon-carbon double bond, and which may be linear or branched or combinations thereof, unless the carbon chain is defined otherwise. Examples of alkenyl include vinyl, allyl, isopropenyl, pentenyl, hexenyl, heptenyl, 1-propenyl, 2-butenyl, 2-methyl-2-butenyl, and the like. Where the specified number of carbon atoms permits, e.g., from C_5-10, the term alkenyl also includes cycloalkenyl groups, and combinations of linear, branched and cyclic structures. When no number of carbon atoms is specified, C_2-6is intended.
“Alkynyl” means carbon chains which contain at least one carbon-carbon triple bond, and which may be linear or branched or combinations thereof. Examples of alkynyl include ethynyl, propargyl, 3-methyl-1-pentynyl, 2-heptynyl, and the like.
“Cycloalkyl” is a subset of alkyl and means a saturated carbocyclic ring having a specified number of carbon atoms. Examples of cycloalkyl include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, and the like. A cycloalkyl group generally is monocyclic unless stated otherwise. Cycloalkyl groups are saturated unless otherwise defined.
The term “alkoxy” refers to straight or branched chain alkoxides of the number of carbon atoms specified (e.g., C_1-6alkoxy), or any number within this range [i.e., methoxy (MeO—), ethoxy, isopropoxy, etc.].
The term “alkylthio” refers to straight or branched chain alkylsulfides of the number of carbon atoms specified (e.g., C_1-6alkylthio), or any number within this range [i.e., methylthio (MeS—), ethylthio, isopropylthio, etc.].
The term “alkylamino” refers to straight or branched alkylamines of the number of carbon atoms specified (e.g., C_1-6alkylamino), or any number within this range [i.e., methylamino, ethylamino, isopropylamino, t-butylamino, etc.].
The term “alkylsulfonyl” refers to straight or branched chain alkylsulfones of the number of carbon atoms specified (e.g., C_1-6alkylsulfonyl), or any number within this range [i.e., methylsulfonyl (MeSO₂—), ethylsulfonyl, isopropylsulfonyl, etc.].
The term “alkylsulfinyl” refers to straight or branched chain alkylsulfoxides of the number of carbon atoms specified (e.g., C_1-6alkylsulfinyl), or any number within this range [i.e., methylsulfinyl (MeSO—), ethylsulfinyl, isopropylsulfinyl, etc.].
The term “alkyloxycarbonyl” refers to straight or branched chain esters of a carboxylic acid derivative of the present invention of the number of carbon atoms specified (e.g., C_1-6alkyloxycarbonyl), or any number within this range [i.e., methyloxycarbonyl (MeOCO—), ethyloxycarbonyl, or butyloxycarbonyl].
“Aryl” means a mono- or polycyclic aromatic ring system containing carbon ring atoms. The preferred aryls are monocyclic or bicyclic 6-10 membered aromatic ring systems. Phenyl and naphthyl are preferred aryls. The most preferred aryl is phenyl.
“Heterocycle” and “heterocyclyl” refer to saturated or unsaturated non-aromatic rings or ring systems containing at least one heteroatom selected from O, S and N, further including the oxidized forms of sulfur, namely SO and SO₂. Examples of heterocycles include tetrahydrofuran (THF), dihydrofuran, 1,4-dioxane, morpholine, 1,4-dithiane, piperazine, piperidine, 1,3-dioxolane, imidazolidine, imidazoline, pyrroline, pyrrolidine, tetrahydropyran, dihydropyran, oxathiolane, dithiolane, 1,3-dioxane, 1,3-dithiane, oxathiane, thiomorpholine, and the like.
“Heteroaryl” means an aromatic or partially aromatic heterocycle that contains at least one ring heteroatom selected from O, S and N. Heteroaryls thus includes heteroaryls fused to other kinds of rings, such as aryls, cycloalkyls and heterocycles that are not aromatic. Examples of heteroaryl groups include: pyrrolyl, isoxazolyl, isothiazolyl, pyrazolyl, pyridyl, oxazolyl, oxadiazolyl, thiadiazolyl, thiazolyl, imidazolyl, triazolyl, tetrazolyl, furyl, triazinyl, thienyl, pyrimidyl, benzisoxazolyl, benzoxazolyl, benzothiazolyl, benzothiadiazolyl, dihydrobenzofuranyl, indolinyl, pyridazinyl, indazolyl, isoindolyl, dihydrobenzothienyl, indolizinyl, cinnolinyl, phthalazinyl, quinazolinyl, naphthyridinyl, carbazolyl, benzodioxolyl, quinoxalinyl, purinyl, furazanyl, isobenzylfuranyl, benzimidazolyl, benzofuranyl, benzothienyl, quinolyl, indolyl, isoquinolyl, dibenzofuranyl, and the like. For heterocyclyl and heteroaryl groups, rings and ring systems containing from 3-15 atoms are included, forming 1-3 rings.
“Halogen” refers to fluorine, chlorine, bromine and iodine. Chlorine and fluorine are generally preferred. Fluorine is most preferred when the halogens are substituted on an alkyl or alkoxy group (e.g. CF₃O and CF₃CH₂O).
The term “composition”, as in pharmaceutical composition, is intended to encompass a product comprising the active ingredient(s), and the inert ingredient(s) that make up the carrier, as well as any product which results, directly or indirectly, from combination, complexation or aggregation of any two or more of the ingredients, or from dissociation of one or more of the ingredients, or from other types of reactions or interactions of one or more of the ingredients. Accordingly, the pharmaceutical compositions of the present invention encompass any composition made by admixing a compound of the present invention and a pharmaceutically acceptable carrier.
The terms “administration of” and “administering a” compound should be understood to mean providing a compound of the invention or a prodrug of a compound of the invention to the individual in need.
Compounds of structural formula I may contain one or more asymmetric centers and can thus occur as racemates and racemic mixtures, single enantiomers, diastereomeric mixtures and individual diastereomers. The present invention is meant to comprehend all such isomeric forms of the compounds of structural formula I.
Some of the compounds described herein contain olefinic double bonds, and unless specified otherwise, are meant to include both E and Z geometric isomers.
Some of the compounds described herein may exist as tautomers such as keto-enol tautomers. The individual tautomers, as well as mixtures thereof, are encompassed within the compounds of structural formula I.
Compounds of structural formula I may be separated into their individual diastereoisomers by, for example, fractional crystallization from a suitable solvent, for example methanol or ethyl acetate or a mixture thereof, or via chiral chromatography using an optically active stationary phase. Absolute stereochemistry may be determined by X-ray crystallography of crystalline products or crystalline intermediates which are derivatized, if necessary, with a reagent containing an asymmetric center of known absolute configuration.
Alternatively, any stereoisomer of a compound of the general structural formula I may be obtained by stereospecific synthesis using optically pure starting materials or reagents of known absolute configuration.
In a different aspect of the invention, a pharmaceutical composition is addressed comprising a compound in accordance with structural formula I, or a pharmaceutically acceptable salt or solvate thereof, in combination with a pharmaceutically acceptable carrier. By the term “solvate” is meant a hydrate, an alcoholate, or other solvate of crystallization.
In another aspect of the invention, a method of treating hyperglycemia, diabetes or insulin resistance in a mammalian patient in need of such treatment is addressed, which comprises administering to said patient an effective amount of a compound in accordance with structural formula I or a pharmaceutically salt or solvate thereof.
In another aspect of the invention, a method of treating non-insulin dependent (Type 2) diabetes mellitus in a mammalian patient in need of such treatment is disclosed comprising administering to the patient an anti-diabetic effective amount of a compound in accordance with structural formula I.
In another aspect of the invention, a method of treating obesity in a mammalian patient in need of such treatment is disclosed comprising administering to said patient a compound in accordance with structural formula I in an amount that is effective to treat obesity.
In another aspect of the invention, a method of treating Metabolic Syndrome in a mammalian patient in need of such treatment is disclosed, comprising administering to said patient a compound in accordance with structural formula I in an amount that is effective to treat Metabolic Syndrome.
In another aspect of the invention, a method of treating a lipid disorder selected from the group consisting of dyslipidemia, hyperlipidemia, hypertriglyceridemia, hypercholesterolemia, low HDL, and high LDL in a mammalian patient in need of such treatment is disclosed, comprising administering to said patient a compound in accordance with structural formula I in an amount that is effective to treat said lipid disorder.
In another aspect of the invention, a method of treating atherosclerosis in a mammalian patient in need of such treatment is disclosed, comprising administering to said patient a compound in accordance with structural formula I in an amount effective to treat atherosclerosis.
In another aspect of the invention, a method of treating a condition selected from the group consisting of: (1) hyperglycemia, (2) low glucose tolerance, (3) insulin resistance, (4) obesity, (5) lipid disorders, (6) dyslipidemia, (7) hyperlipidemia, (8) hypertriglyceridemia, (9) hypercholesterolemia, (10) low HDL levels, (11) high LDL levels, (12) atherosclerosis and its sequelae, (13) vascular restenosis, (14) pancreatitis, (15) abdominal obesity, (16) neurodegenerative disease, (17) retinopathy, (18) nephropathy, (19) neuropathy, (20) Metabolic Syndrome, (21) hypertension and other conditions and disorders where insulin resistance is a component, in a mammalian patient in need of such treatment is disclosed, comprising administering to the patient a compound in accordance with structural formula I in an amount that is effective to treat said condition.
In another aspect of the invention, a method of delaying the onset of a condition selected from the group consisting of (1) hyperglycemia, (2) low glucose tolerance, (3) insulin resistance, (4) obesity, (5) lipid disorders, (6) dyslipidemia, (7) hyperlipidemia, (8) hypertriglyceridemia, (9) hypercholesterolemia, (10) low HDL levels, (11) high LDL levels, (12) atherosclerosis and its sequelae, (13) vascular restenosis, (14) pancreatitis, (15) abdominal obesity, (16) neurodegenerative disease, (17) retinopathy, (18) nephropathy, (19) neuropathy, (20) Metabolic Syndrome, (21) hypertension and other conditions and disorders where insulin resistance is a component in a mammalian patient in need of such treatment is disclosed, comprising administering to the patient a compound in accordance with structural formula I in an amount that is effective to delay the onset of said condition.
In another aspect of the invention, a method of reducing the risk of developing a condition selected from the group consisting of (1) hyperglycemia, (2) low glucose tolerance, (3) insulin resistance, (4) obesity, (5) lipid disorders, (6) dyslipidemia, (7) hyperlipidemia, (8) hypertriglyceridemia, (9) hypercholesterolemia, (10) low HDL levels, (11) high LDL levels, (12) atherosclerosis and its sequelae, (13) vascular restenosis, (14) pancreatitis, (15) abdominal obesity, (16) neurodegenerative disease, (17) retinopathy, (18) nephropathy, (19) neuropathy, (20) Metabolic Syndrome, (21) hypertension and other conditions and disorders where insulin resistance is a component in a mammalian patient in need of such treatment is disclosed, comprising administering to the patient a compound in accordance with structural formula I in an amount that is effective to reduce the risk of developing said condition.
In another aspect of the invention, a method of treating a condition selected from the group consisting of (1) hyperglycemia, (2) low glucose tolerance, (3) insulin resistance, (4) obesity, (5) lipid disorders, (6) dyslipidemia, (7) hyperlipidemia, (8) hypertriglyceridemia, (9) hypercholesterolemia, (10) low HDL levels, (11) high LDL levels, (12) atherosclerosis and its sequelae, (13) vascular restenosis, (14) pancreatitis, (15) abdominal obesity, (16) neurodegenerative disease, (17) retinopathy, (18) nephropathy, (19) neuropathy, (20) Metabolic Syndrome, (21) hypertension and other conditions and disorders where insulin resistance is a component, in a mammalian patient in need of such treatment, comprising administering to the patient an effective amount of a compound as defined in structural formula I and a compound selected from the group consisting of:
(a) dipeptidyl peptidase-IV (DP-IV) inhibitors;
(b) insulin sensitizing agents selected from the group consisting of (i) PPARγ agonists, (ii) PPARα agonists, (iii) PPARα/γ dual agonists, and (iv) biguanides;
(c) insulin and insulin mimetics;
(d) sulfonylureas and other insulin secretagogues;
(e) α-glucosidase inhibitors;
(f) glucagon receptor antagonists;
(g) GLP-1, GLP-1 analogs, and GLP-1 receptor agonists;
(h) GIP, GIP mimetics, and GIP receptor agonists;
(i) PACAP, PACAP mimetics, and PACAP receptor 3 agonists;
(j) cholesterol lowering agents selected from the group consisting of

- (i) HMG-CoA reductase inhibitors, (ii) sequestrants, (iii) nicotinyl alcohol, nicotinic acid and salts thereof, (iv) inhibitors of cholesterol absorption, (v) acyl CoA:cholesterol acyltransferase inhibitors, and (vi) anti-oxidants;

(k) PPARδ agonists;
(l) antiobesity compounds;
(m) ileal bile acid transporter inhibitors;
(n) anti-inflammatory agents, excluding glucocorticoids;
(O) protein tyrosine phosphatase 1B (PTP-1B) inhibitors; and
(p) antihypertensives including those acting on the angiotensin or renin systems, such as angiotensin converting enzyme inhibitors, angiotensin II receptor antagonists or renin inhibitors, such as captopril, cilazapril, enalapril, fosinopril, lisinopril, quinapril, ramapril, zofenopril, candesartan, cilexetil, eprosartan, irbesartan, losartan, tasosartan, telmisartan, and valsartan;
said compounds being administered to the patient in an amount that is effective to treat said condition.
Dipeptidyl peptidase-IV inhibitors that can be combined with compounds of structural formula I include those disclosed in WO 03/004498 (16 Jan. 2003); WO 03/004496 (16 Jan. 2003); EP 1 258 476 (20 Nov. 2002); WO 02/083128 (24 Oct. 2002); WO 02/062764 (15 Aug. 2002); WO 03/000250 (3 Jan. 2003); WO 03/002530 (9 Jan. 2003); WO 03/002531 (9
Jan. 2003); WO 03/002553 (9 Jan. 2003); WO 03/002593 (9 Jan. 2003); WO 03/000180 (3 Jan. 2003); and WO 03/000181 (3 Jan. 2003). Specific DP-IV inhibitor compounds include isoleucine thiazolidide; NVP-DPP728; P32/98; and LAF 237.
Antiobesity compounds that can be combined with compounds of structural formula I include fenfluramine, dexfenfluramine, phentermine, sibutramine, orlistat, neuropeptide Y₁or Y₅antagonists, cannabinoid CB1 receptor antagonists or inverse agonists, melanocortin receptor agonists, in particular, melanocortin-4 receptor agonists, ghrelin antagonists, and melanin-concentrating hormone (MCH) receptor antagonists. For a review of anti-obesity compounds that can be combined with compounds of structural formula I, see S. Chaki et al., “Recent advances in feeding suppressing agents: potential therapeutic strategy for the treatment of obesity,” Expert Opin. Ther. Patents, 11: 1677-1692 (2001)
Neuropeptide Y5 antagonists that can be combined with compounds of structural formula I include those disclosed in U.S. Pat. No. 6,335,345 (1 Jan. 2002) and WO 01/14376 (1 Mar. 2001); and specific compounds identified as GW 59884A; GW 569180A; LY366377; and CGP-71683A.
Cannabinoid CB1 receptor antagonists that can be combined with compounds of formula I include those disclosed in PCT Publication WO 03/007887; U.S. Pat. No. 5,624,941, such as rimonabant; PCT Publication WO 02/076949, such as SLV-319; U.S. Pat. No. 6,028,084; PCT Publication WO 98/41519; PCT Publication WO 00/10968; PCT Publication WO 99/02499; U.S. Pat. No. 5,532,237; and U.S. Pat. No. 5,292,736.
Melanocortin receptor agonists that can be combined with compounds of structural formula I include those disclosed in WO 03/009847 (6 Feb. 2003); WO 02/068388 (6 Sep. 2002); WO 99/64002 (16 Dec. 1999); WO 00/74679 (14 Dec. 2000); WO 01/70708 (27 Sep. 2001); and WO 01/70337 (27 Sep. 2001) as well as those disclosed in J. D. Speake et al., “Recent advances in the development of melanocortin-4 receptor agonists, Expert Opin. Ther. Patents, 12: 1631-1638 (2002).
In another aspect of the invention, a method of treating a condition selected from the group consisting of hypercholesterolemia, atherosclerosis, low HDL levels, high LDL levels, hyperlipidemia, hypertriglyceridemia, and dyslipidemia, in a mammalian patient in need of such treatment is disclosed, comprising administering to the patient a therapeutically effective amount of a compound as defined in structural formula I and an HMG-CoA reductase inhibitor.
More particularly, in another aspect of the invention, a method of treating a condition selected from the group consisting of hypercholesterolemia, atherosclerosis, low HDL levels, high LDL levels, hyperlipidemia, hypertriglyceridemia and dyslipidemia, in a mammalian patient in need of such treatment is disclosed, wherein the HMG-CoA reductase inhibitor is a statin.
Even more particularly, in another aspect of the invention, a method of treating a condition selected from the group consisting of hypercholesterolemia, atherosclerosis, low HDL levels, high LDL levels, hyperlipidemia, hypertriglyceridemia and dyslipidemia, in a mammalian patient in need of such treatment is disclosed, wherein the HMG-CoA reductase inhibitor is a statin selected from the group consisting of lovastatin, simvastatin, pravastatin, cerivastatin, fluvastatin, atorvastatin, itavastatin, and rosuvastatin.
In another aspect of the invention, a method of reducing the risk of developing a condition selected from the group consisting of hypercholesterolemia, atherosclerosis, low HDL levels, high LDL levels, hyperlipidemia, hypertriglyceridemia and dyslipidemia, and the sequelae of such conditions is disclosed comprising administering to a mammalian patient in need of such treatment a therapeutically effective amount of a compound as defined in structural formula I and an HMG-CoA reductase inhibitor.
In another aspect of the invention, a method for delaying the onset or reducing the risk of developing atherosclerosis in a human patient in need of such treatment is disclosed comprising administering to said patient an effective amount of a compound as defined in structural formula I and an HMG-CoA reductase inhibitor.
More particularly, a method for delaying the onset or reducing the risk of developing atherosclerosis in a human patient in need of such treatment is disclosed, wherein the HMG-CoA reductase inhibitor is a statin.
Even more particularly, a method for delaying the onset or reducing the risk of developing atherosclerosis in a human patient in need of such treatment is disclosed, wherein the HMG-Co A reductase inhibitor is a statin selected from the group consisting of: lovastatin, simvastatin, pravastatin, cerivastatin, fluvastatin, atorvastatin, itavastatin, and rosuvastatin.
Even more particularly, a method for delaying the onset or reducing the risk of developing atherosclerosis in a human patient in need of such treatment is disclosed, wherein the statin is simvastatin.
In another aspect of the invention, a method for delaying the onset or reducing the risk of developing atherosclerosis in a human patient in need of such treatment is disclosed, wherein the HMG-CoA reductase inhibitor is a statin and further comprising administering a cholesterol absorption inhibitor.
More particularly, in another aspect of the invention, a method for delaying the onset or reducing the risk of developing atherosclerosis in a human patient in need of such treatment is disclosed, wherein the HMG-Co A reductase inhibitor is a statin and the cholesterol absorption inhibitor is ezetimibe.
In another aspect of the invention, a pharmaceutical composition is disclosed which comprises
(1) a compound according to structural formula I,
(2) a compound selected from the group consisting of:
(a) DP-IV inhibitors;
(b) insulin sensitizing agents selected from the group consisting of (i) PPARγ agonists; (ii) PPARα agonists, (iii) PPARα/γ dual agonists, and (iv) biguanides;
(c) insulin and insulin mimetics;
(d) sulfonylureas and other insulin secretagogues;
(e) α-glucosidase inhibitors;
(f) glucagon receptor antagonists;
(g) GLP-1, GLP-1 analogs, and GLP-1 receptor agonists;
(h) GIP, GIP mimetics, and GIP receptor agonists;
(i) PACAP, PACAP mimetics, and PACAP receptor 3 agonists;
k) cholesterol lowering agents selected from the group consisting of (i) HMG-CoA reductase inhibitors, (ii) sequestrants, (iii) nicotinyl alcohol, nicotinic acid or a salt thereof, (iv) inhibitors of cholesterol absorption, (v) acyl CoA:cholesterol acyltransferase inhibitors, and (vi) anti-oxidants;
(k) PPARδ agonists;
(l) antiobesity compounds;
(m) ileal bile acid transporter inhibitors;
(n) anti-inflammatory agents other than glucocorticoids;
(O) protein tyrosine phosphatase 1B (PTP-1B) inhibitors; and
(p) antihypertensives including those acting on the angiotensin or renin systems, such as angiotensin converting enzyme inhibitors, angiotensin II receptor antagonists or renin inhibitors, such as captopril, cilazapril, enalapril, fosinopril, lisinopril, quinapril, ramapril, zofenopril, candesartan, cilexetil, eprosartan, irbesartan, losartan, tasosartan, telmisartan, and valsartan; and
(3) a pharmaceutically acceptable carrier.
The compounds of the present invention may be administered in the form of a pharmaceutically acceptable salt. The term “pharmaceutically acceptable salt” refers to salts prepared from pharmaceutically acceptable non-toxic bases or acids including inorganic or organic bases and inorganic or organic acids. Salts of basic compounds encompassed within the term “pharmaceutically acceptable salt” refer to non-toxic salts of the compounds of this invention which are generally prepared by reacting the free base with a suitable organic or inorganic acid. Representative salts of basic compounds of the present invention include, but are not limited to, the following: acetate, benzenesulfonate, benzoate, bicarbonate, bisulfate, bitartrate, borate, bromide, camsylate, carbonate, chloride, clavulanate, citrate, dihydrochloride, edetate, edisylate, estolate, esylate, fumarate, gluceptate, gluconate, glutamate, glycollylarsanilate, hexylresorcinate, hydrabamine, hydrobromide, hydrochloride, hydroxynaphthoate, iodide, isothionate, lactate, lactobionate, laurate, malate, maleate, mandelate, mesylate, methylbromide, methylnitrate, methylsulfate, mucate, napsylate, nitrate, N-methylglucamine ammonium salt, oleate, oxalate, pamoate (embonate), palmitate, pantothenate, phosphate/diphosphate, polygalacturonate, salicylate, stearate, sulfate, subacetate, succinate, tannate, tartrate, teoclate, tosylate, triethiodide and valerate. Furthermore, where the compounds of the invention carry an acidic moiety, suitable pharmaceutically acceptable salts thereof include, but are not limited to, salts derived from inorganic bases including aluminum, ammonium, calcium, copper, ferric, ferrous, lithium, magnesium, manganic, mangamous, potassium, sodium, zinc, and the like. Particularly preferred are the ammonium, calcium, magnesium, potassium, and sodium salts. Salts derived from pharmaceutically acceptable organic non-toxic bases include salts of primary, secondary, and tertiary amines, cyclic amines, and basic ion-exchange resins, such as arginine, betaine, caffeine, choline, N,N-dibenzylethylenediamine, diethylamine, 2-diethylaminoethanol, 2-dimethylaminoethanol, ethanolamine, ethylenediamine, N-ethylmorpholine, N-ethylpiperidine, glucamine, glucosamine, histidine, hydrabamine, isopropylamine, lysine, methylglucamine, morpholine, piperazine, piperidine, polyamine resins, procaine, purines, theobromine, triethylamine, trimethylamine, tripropylamine, tromethamine, and the like.
Also, in the case of a carboxylic acid (—COOH) or alcohol group being present in the compounds of the present invention, pharmaceutically acceptable esters of carboxylic acid derivatives, such as methyl, ethyl, or pivaloyloxymethyl, or acyl derivatives of alcohols, such as acetate or maleate, can be employed. Included are those esters and acyl groups known in the art for modifying the solubility or hydrolysis characteristics for use as sustained-release or prodrug formulations.
It will be understood that, as used herein, references to the compounds of structural formula I are meant to also include the pharmaceutically acceptable salts, and also salts that are not pharmaceutically acceptable when they are used as precursors to the free compounds or their pharmaceutically acceptable salts or in other synthetic manipulations.
Solvates, and in particular, the hydrates of the compounds of structural formula I are included in the present invention as well.
The compounds described herein are selective inhibitors of the 11β-HSD1 enzyme. Thus, the present invention relates to the use of the 11β-HSD1 inhibitors for inhibiting the reductase activity of 11β-hydroxysteroid dehydrogenase, which is responsible for the conversion of cortisone to cortisol. Excess cortisol is associated with numerous disorders, including NIDDM, obesity, dyslipidemia, insulin resistance and hypertension. Administration of the compounds of the present invention decreases the level of cortisol and other 11β-hydroxysteroids in target tissues, thereby reducing the effects of excessive amounts of cortisol and other 11β-hydroxysteroids. Inhibition of 11β-HSD1 can be used to treat and control diseases mediated by abnormally high levels of cortisol and other 11β-hydroxysteroids, such as NIDDM, obesity, hypertension and dyslipidemia. Inhibition of 11β-HSD1 activity in the brain such as to lower cortisol levels may also be useful to treat or reduce anxiety, depression, and cognitive impairment.
The present invention includes the use of an 11β-HSD1 inhibitor for the treatment, control, amelioration, prevention, delaying the onset of or reducing the risk of developing the diseases and conditions that are described herein, as mediated by excess or uncontrolled amounts of cortisol and/or other corticosteroids in a mammalian patient, particularly a human, by the administration of an effective amount of a compound of structural formula I or a pharmaceutically acceptable salt or solvate thereof. Inhibition of the 11β-HSD1 enzyme limits the conversion of cortisone, which is normally inert, to cortisol, which can cause or contribute to the symptoms of these diseases and conditions if present in excessive amounts.

NIDDM and Hypertension:

The compounds of this invention are selective inhibitors of 11β-HSD1 over 11β-HSD2. While the inhibition of 11β-HSD1 is useful for reducing cortisol levels and treating conditions related thereto, inhibition of 11β-HSD2 is associated with serious side effects, such as hypertension.
Cortisol is an important and well recognized anti-inflammatory hormone, which also acts as an antagonist to the action of insulin in the liver, such that insulin sensitivity is reduced, resulting in increased gluconeogenesis and elevated levels of glucose in the liver. Patients who already have impaired glucose tolerance have a greater probability of developing Type 2 diabetes in the presence of abnormally high levels of cortisol.
High levels of cortisol in tissues where the mineralocorticoid receptor is present often lead to hypertension. Inhibition of 11β-HSD1 shifts the ratio of cortisol and cortisone in specific tissues in favor of cortisone.
Administration of a therapeutically effective amount of an 11β-HSD1 inhibitor is effective in treating, controlling and ameliorating the symptoms of NIDDM, and administration of a therapeutically effective amount of an 11β-HSD1 inhibitor on a regular basis delays or prevents the onset of NIDDM, particularly in humans.

Cushing's Syndrome:

The effect of elevated levels of cortisol is also observed in patients who have Cushing's Syndrome, which is a metabolic disease characterized by high levels of cortisol in the blood stream. Patients with Cushing's Syndrome often develop NIDDM.

Obesity Metabolic Syndrome Dyslipidemia:

Excessive levels of cortisol have been associated with obesity, perhaps due to increased hepatic gluconeogenesis. Abdominal obesity is closely associated with glucose intolerance, hyperinsulinemia, hypertriglyceridemia, and other factors of Metabolic Syndrome, such as high blood pressure, elevated VLDL and reduced HDL. Montague et al., Diabetes, 2000, 49: 883-888. Thus, the administration of an effective amount of an 11β-HSD1 inhibitor is useful in the treatment or control of obesity. Long-term treatment with an 11β-HSD1 inhibitor is also useful in delaying or preventing the onset of obesity, especially if the patient uses an 11β-HSD1 inhibitor in combination with controlled diet and exercise.
By reducing insulin resistance and maintaining serum glucose at normal concentrations, compounds of the present invention also have utility in the treatment and prevention of conditions that accompany Type II diabetes and insulin resistance, including the Metabolic Syndrome or Syndrome X, obesity, reactive hypoglycemia and diabetic dyslipidemia.

Cognition and Dementia:

Excessive levels of cortisol in the brain may also result in neuronal loss or dysfunction through the potentiation of neurotoxins. Cognitive impairment has been associated with aging, and excess levels of cortisol in the brain. See J. R. Seckl and B. R. Walker, Endocrinology, 2001, 142: 1371-1376, and references cited therein. Administration of an effective amount of an 11β-HSD1 inhibitor results in the reduction, amelioration, control or prevention of cognitive impairment associated with aging and of neuronal dysfunction. Inhibitors of 11β-HSD1 may also be useful to treat anxiety and depression.

Atherosclerosis:

As described above, inhibition of 113-HSD1 activity and a reduction in the amount of cortisol are beneficial in treating or controlling hypertension. Since hypertension and dyslipidemia contribute to the development of atherosclerosis, administration of a therapeutically effective amount of an 11β-HSD1 inhibitor of the present invention may be especially beneficial in treating, controlling, delaying the onset of or preventing atherosclerosis.

Effects on Pancreas:

Inhibition of 11β-HSD1 activity in isolated murine pancreatic 1-cells improves glucose stimulated insulin secretion (B. Davani et al., J. Biol. Chem., 2000, 275: 34841-34844). Glucocorticoids have been shown to reduce insulin secretion in vivo. (B. Billaudel et al., Horm. Metab. Res., 1979, 11: 555-560).

Reduction of Intraocular Pressure:

Recent data suggests a connection between the levels of glucocorticoid target receptors and the 11β-HSD enzymes and the susceptibility to glaucoma (J. Stokes et al., Invest. Ophthamol., 2000, 41: 1629-1638). Therefore, inhibition of 11β-HSD1 activity is useful in reducing intraocular pressure in the treatment of glaucoma.

Immunomodulation:

In certain disease states, such as tuberculosis, psoriasis, and even under conditions of excessive stress, high glucocorticoid activity shifts the immune response to a humoral response, when in fact a cell based response may be more beneficial to the patient. Inhibition of 11β-HSD1 activity and the attendant reduction in glucocorticoid levels shifts the immune response toward a cell based response. See D. Mason, Immunology Today, 1991, 12: 57-60, and G. A. W. Rook, Bailliér's Clin. Endocrinol. Metab., 1999, 13: 576-581.

Osteoporosis:

Glucocorticoids can inhibit bone formation, which can result in a net bone loss. 11β-HSD1 has a role in bone resorption. Inhibition of 11β-HSD1 is beneficial in preventing bone loss due to osteoporosis. See C. H. Kim et al., J. Endocrinol., 1999, 162: 371-379; C. G. Bellows et al., Bone, 1998, 23: 119-125; and M. S. Cooper et al., Bone, 2000, 27: 375-381.

Other Utilities:

The following diseases, disorders and conditions can be treated, controlled, prevented or delayed, by treatment with the compounds of this invention: (1) hyperglycemia, (2) low glucose tolerance, (3) insulin resistance, (4) obesity, (5) lipid disorders, (6) dyslipidemia, (7) hyperlipidemia, (8) hypertriglyceridemia, (9) hypercholesterolemia, (10) low HDL levels, (11) high LDL levels, (12) atherosclerosis and its sequelae, (13) vascular restenosis, (14) pancreatitis, (15) abdominal obesity, (16) neurodegenerative disease, (17) retinopathy, (18) nephropathy, (19) neuropathy, (20) Metabolic Syndrome, (21) hypertension and other disorders where insulin resistance is a component.
The above diseases and conditions can be treated using the compounds of structural formula I, or the compound can be administered to prevent or reduce the risk of developing the diseases and conditions described herein. Since concurrent inhibition of 11β-HSD2 may have deleterious side effects or may actually increase the amount of cortisol in the target tissue where reduction of cortisol is desired, selective inhibitors of 11β-HSD1 with little or no inhibition of 11β-HSD2 are desirable.
The 11β-HSD1 inhibitors of structural formula I generally have an inhibition constant IC₅₀of less than about 500 nM, and preferably less than about 100 nM. Generally, the IC₅₀ratio for 11β-HSD2 to 11β-HSD1 of a compound is at least about two or more, and preferably about ten or greater. Even more preferred are compounds with an IC₅₀ratio for 11β-HSD2 to 11β-HSD1 of about 100 or greater. For example, compounds of the present invention ideally demonstrate an inhibition constant IC₅₀against 11β-HSD2 greater than about 1000 nM, and preferably greater than 5000 nM.
Compounds of structural formula I may be used in combination with one or more other drugs in the treatment, prevention, suppression or amelioration of diseases or conditions for which compounds of structural formula I or the other drugs have utility. Typically the combination of the drugs is safer or more effective than either drug alone, or the combination is safer or more effective than would be expected based on the additive properties of the individual drugs. Such other drug(s) may be administered, by a route and in an amount commonly used contemporaneously or sequentially with a compound of structural formula I. When a compound of structural formula I is used contemporaneously with one or more other drugs, a combination product containing such other drug(s) and the compound of structural formula I is preferred. However, combination therapy also includes therapies in which the compound of structural formula I and one or more other drugs are administered on different overlapping schedules. It is contemplated that when used in combination with other active ingredients, the compound of the present invention or the other active ingredient or both may be used effectively in lower doses than when each is used alone. Accordingly, the pharmaceutical compositions of the present invention include those that contain one or more other active ingredients, in addition to a compound of structural formula I.
Examples of other active ingredients that may be administered in combination with a compound of structural formula I, and either administered separately or in the same pharmaceutical composition, include, but are not limited to:
(a) dipeptidyl peptidase IV (DP-IV) inhibitors;
(b) insulin sensitizing agents including (i) PPARγ agonists such as the glitazones (e.g. troglitazone, pioglitazone, englitazone, MCC-555, rosiglitazone, and the like) and other PPAR ligands, including PPARα/γ dual agonists, such as KRP-297, and PPARα agonists such as gemfibrozil, clofibrate, fenofibrate and bezafibrate, and (ii) biguanides, such as metformin and phenformin;
(c) insulin or insulin mimetics;
(d) sulfonylureas and other insulin secretagogues such as tolbutamide, glipizide, meglitinide and related materials;
(e) α-glucosidase inhibitors, such as acarbose;
(f) glucagon receptor antagonists such as those disclosed in WO 98/04528, WO 99/01423, WO 00/39088 and WO 00/69810;
(g) GLP-1, GLP-1 analogs, and GLP-1 receptor agonists such as those disclosed in WO00/42026 and WO00/59887;
(h) GIP, GIP mimetics such as those disclosed in WO00/58360, and GIP receptor agonists;
(i) PACAP, PACAP mimetics, and PACAP receptor 3 agonists such as those disclosed in WO 01/23420;
k) cholesterol lowering agents such as (i) HMG-CoA reductase inhibitors (lovastatin, simvastatin, pravastatin, cerivastatin, fluvastatin, atorvastatin, itavastatin, rosuvastatin, and other statins), (ii) bile-acid sequestrants (cholestyramine, colestipol, and dialkylaminoalkyl derivatives of a cross-linked dextran), (iii) nicotinyl alcohol, nicotinic acid or a salt thereof, (iv) inhibitors of cholesterol absorption, such as ezetimibe and beta-sitosterol, (v) acyl CoA:cholesterol acyltransferase inhibitors, such as, for example, avasimibe, and (vi) anti-oxidants, such as probucol;
(k) PPARδ agonists, such as those disclosed in WO97/28149;
(l) antiobesity compounds such as fenfluramine, dexfenfluramine, phentermine, sibutramine, orlistat, neuropeptide Y₁or Y₅antagonists, CB1 receptor inverse agonists and antagonists, β₃adrenergic receptor agonists, melanocortin-receptor agonists, in particular melanocortin-4 receptor agonists, ghrelin antagonists, and melanin-concentrating hormone (MCH) receptor antagonists;
(m) ileal bile acid transporter inhibitors;
(n) agents intended for use in inflammatory conditions other than glucocorticoids, such as aspirin, non-steroidal anti-inflammatory drugs, azulfidine, and selective cyclooxygenase-2 inhibitors;
(O) protein tyrosine phosphatase 1B (PTP-1B) inhibitors; and
(p) antihypertensives including those acting on the angiotensin or renin systems, such as angiotensin converting enzyme inhibitors, angiotensin II receptor antagonists or renin inhibitors, such as captopril, cilazapril, enalapril, fosinopril, lisinopril, quinapril, ramapril, zofenopril, candesartan, cilexetil, eprosartan, irbesartan, losartan, tasosartan, telmisartan, and valsartan.
The above combinations include a compound of structural formula I, or a pharmaceutically acceptable salt or solvate thereof, with one or more other active compounds. Non-limiting examples include combinations of compounds of structural formula I with two or more active compounds selected from biguanides, sulfonylureas, HMG-CoA reductase inhibitors, PPAR agonists, PTP-1B inhibitors, DP-IV inhibitors, and anti-obesity compounds.
Any suitable route of administration may be employed for providing a mammal, especially a human, with an effective dose of a compound of the present invention. For example, oral, rectal, topical, parenteral, ocular, pulmonary, nasal and the like may be employed. Dosage forms include tablets, troches, dispersions, suspensions, solutions, capsules, creams, ointments, aerosols and the like. Preferably the compound of structural formula I is administered orally.
The effective dosage of the active ingredient varies depending on the particular compound employed, the mode of administration, the condition being treated and the severity of the condition. Such dosages may be ascertained readily by a person skilled in the art.
When treating or preventing the diseases and conditions described herein, for which compounds of structural formula I are indicated, satisfactory results are obtained when the compounds of the invention are administered at a daily dosage of from about 0.1 to about 100 milligram per kilogram (mpk) of body weight, preferably given as a single daily dose or in divided doses about two to six times a day. The total daily dosage thus ranges from about 0.1 mg to about 1000 mg, preferably from about 1 mg to about 50 mg. In the case of a typical 70 kg adult human, the total daily dose will range from about 7 mg to about 350 mg. This dosage may be adjusted to provide the optimal therapeutic response.
Another aspect of the present invention relates to a pharmaceutical composition which comprises a compound of structural formula I, or a pharmaceutically acceptable salt or solvate thereof, in combination with a pharmaceutically acceptable carrier.
The compositions include compositions suitable for oral, rectal, topical, parenteral (including subcutaneous, intramuscular, and intravenous), ocular (ophthalmic), transdermal, pulmonary (nasal or buccal inhalation), or nasal administration, although the most suitable route in any given case will depend on the nature and severity of the condition being treated and the active ingredient. They may be conveniently presented in unit dosage form and prepared by any of the methods well-known in the art of pharmacy.
The compound of structural formula I can be combined with the pharmaceutical carrier according to conventional pharmaceutical compounding techniques. Carriers take a wide variety of forms. For example, carriers for oral liquid compositions include, e.g., water, glycols, oils, alcohols, flavoring agents, preservatives, coloring agents and other components used in the manufacture of oral liquid suspensions, elixirs and solutions. Carriers such as starches, sugars and microcrystalline cellulose, diluents, granulating agents, lubricants, binders, disintegrating agents and the like are used to prepare oral solid dosage forms, e.g., powders, hard and soft capsules and tablets. Solid oral preparations are preferred over oral liquids.
The oral solid dosage forms may also contain a binder such as gum tragacanth, acacia, corn starch or gelatin; excipients such as dicalcium phosphate; a disintegrating agent such as corn starch, potato starch, alginic acid; a lubricant such as magnesium stearate; and a sweetening agent such as sucrose, lactose or saccharin. Capsules may also contain a liquid carrier such as a fatty oil.
Various other materials may be present to act as coatings or to modify the physical form of the dosage unit. For instance, tablets may be coated with shellac, sugar or both.
Tablets may be coated by standard aqueous or nonaqueous techniques. The typical percentage of active compound in these compositions may, of course, be varied from about 2 percent to about 60 percent on a w/w basis. Thus, tablets contain a compound of structural formula I or a salt or hydrate thereof in an amount ranging from as low as about 0.1 mg to as high as about 1.5 g, preferably from as low as about 1.0 mg to as high as about 500 mg, and more preferably from as low as about 10 mg to as high as about 100 mg.
Oral liquids such as syrups or elixirs may contain, in addition to the active ingredient, sucrose as a sweetening agent, methyl and propylparabens as preservatives, a dye and a flavoring such as cherry or orange flavor.
Parenterals are typically in the form of a solution or suspension, typically prepared with water, and optionally including a surfactant such as hydroxypropylcellulose. Dispersions can be prepared in glycerol, liquid polyethylene glycols and mixtures thereof in oils. Typically preparations that are in diluted form also contain a preservative.
The pharmaceutical injectable dosage forms, including aqueous solutions and dispersions and powders for the extemporaneous preparation of injectable solutions or dispersions, are also sterile and must be fluid to the extent that easy syringability exists; they must be stable under the conditions of manufacture and storage and are usually preserved. The carrier thus includes the solvent or dispersion medium containing, for example, water, ethanol, polyol (e.g. glycerol, propylene glycol and liquid polyethylene glycol), suitable mixtures thereof, and vegetable oils.

Assays: Measurement of Inhibition Constants:

In vitro enzymatic activity was assessed for test compounds via a Scintillation Proximity Assay (SPA). In short, tritiated-cortisone substrate, NADPH cofactor and titrated compound of structural formula I were incubated with 11β-HSD1 enzyme at 37° C. to allow conversion to cortisol to progress. Following this incubation, a preparation of protein A coated SPA beads, pre-blended with anti-cortisol monoclonal antibody and a non-specific 11β-HSD inhibitor, such as 18β-glycyrrhetinic acid, was added to each well. The mixture was shaken at 15° C. and was then read on a liquid scintillation counter suitable for 96 well plates. Percent inhibition was calculated relative to a non-inhibited control well and IC₅₀curves were generated. This assay was similarly applied to 11β-HSD2, whereby tritiated cortisol and NAD were used as the substrate and cofactor, respectively. To begin the assay, 40 μL of substrate (25 nM ³H-Cortisone+1.25 mM NADPH in 50 mM HEPES Buffer, pH 7.4) was added to designated wells on a 96-well plate. The compound was dissolved in DMSO at 10 mM followed by a subsequent 50 fold dilution in DMSO. The diluted material was then titrated 4 fold, seven times. 1 μL of each titrated compound was then added in duplicate to the substrate. To start the reaction, 10 μL of 11β-HSD1 microsome from CHO transfectants was added to each well at the appropriate concentration to yield approximately 10% conversion of the starting material. For ultimate calculation of percent inhibition, a series of wells were added that represented the assay minimum and maximum: one set that contained substrate without compound or enzyme (background), and another set that contained substrate and enzyme without any compound (maximum signal). The plates were spun briefly at a low speed in a centrifuge to pool the reagents, sealed with an adhesive strip, mixed gently, and incubated at 37° C. for 2 h. After incubation, 45 μL of SPA beads, pre-suspended with anti-cortisol monoclonal antibody and a compound of formula I, were added to each well. The plates were resealed and shaken gently for greater than 1.5 h at 15° C. Data were collected on a plate based liquid scintillation counter such as a Topcount. To control for inhibition of anti-cortisol antibody/cortisol binding, substrate spiked with 1.25 nM [3]H cortisol was added to designated single wells. 1 μL of 200 μM compound was added to each of these wells, along with 10 μL of buffer instead of enzyme. Any calculated inhibition was due to compound interfering with the cortisol binding to the antibody on the SPA beads.

Assays: Measurement of In Vivo Inhibition:

In general terms, the test compound was dosed orally to a mammal and a prescribed time interval was allowed to elapse, usually between 1 and 24 h. Tritiated cortisone was injected intravenously, followed several min later by blood collection. Steroids were extracted from the separated serum and analyzed by HPLC. The relative levels of ³H-cortisone and its reduction product, ³H-cortisol, were determined for the compound and vehicle-dosed control groups. The absolute conversion, as well as the percentage of inhibition, was calculated from these values.
More specifically, compounds were prepared for oral dosing by dissolving them in vehicle (5% hydroxypropyl-beta-cyclodextrin v/v H₂O, or equivalent) at the desired concentration to allow dosing at typically 10 mg per kg. Following an overnight fasting, the solutions were dosed to ICR mice (obtained from Charles River) by oral gavage, 0.5 mL per dose per animal, with three animals per test group.
After the desired time had passed, routinely either 4 or 16 h, 0.2 mL of 3 μM ³H-cortisone in dPBS was injected by tail vein. The animal was caged for two min followed by euthanasia in a CO₂chamber. Upon expiration, the mouse was removed and blood was collected by cardiac puncture. The blood was set aside in a serum separation tube for no less than 30 min at room temperature to allow for adequate coagulation. After the incubation period, blood was separated into serum by centrifugation at 3000×g, 4° C., for 10 min.
To analyze the steroids in the serum, they were first extracted with organic solvent. A 0.2 mL volume of serum was transferred to a clean microcentrifuge tube. To this a 1.0 mL volume of ethyl acetate was added, followed by vigorous vortexing for 1 min. A quick spin on a microcentrifuge pelleted the aqueous serum proteins and clarified the organic supernatant. 0.85 mL of the upper organic phase was transferred to a fresh microcentrifuge tube and dried. The dried sample was resuspended in 0.250 mL of DMSO containing a high concentration of cortisone and cortisol for analysis by HPLC.
A 0.200 mL sample was injected onto a Metachem Inertsil C-18 chromatography column equilibrated in 30% methanol. A slow linear gradient to 50% methanol separated the target steroids; simultaneous monitoring by UV at 254 nm of the cold standards in the resuspension solution acted as an internal standard. The tritium signal was collected by a radiochromatography detector that uploaded data to software for analysis. The percent conversion of ³H-cortisone to ³H-cortisol was calculated as the ratio of AUC for cortisol over the combined AUC for cortisone and cortisol.

Preparation of Compounds of the Invention:

The compounds of structural formula I of the present invention can be prepared according to the procedures of the following Schemes and Examples, using appropriate materials and are further exemplified by the following specific examples. The compounds illustrated in the examples are not, however, to be construed as forming the only genus that is considered as the invention. The Examples further illustrate details for the preparation of the compounds of the present invention. Those skilled in the art will readily understand that known variations of the conditions and processes of the following preparative procedures can be used to prepare these compounds. The instant compounds are generally isolated in the their neutral form, but the triazole moeity can be further converted into a pharmaceutically acceptable salt by dissolution in an organic solvent followed by addition of the appropriate acid and subsequent evaporation, precipitation, or crystallization. All temperatures are degrees Celsius unless otherwise noted. Mass spectra (MS) were measured by electrospray ion-mass spectroscopy (ESMS).
The phrase “standard peptide coupling reaction conditions” means coupling a carboxylic acid with an amine using an acid activating agent such as EDC, DCC, and BOP in an inert solvent such as dichloromethane in the presence of a catalyst such as HOBT. The use of protecting groups for the amine and carboxylic acid functionalities to facilitate the desired reaction and minimize undesired reactions is well documented. Conditions required to remove protecting groups are found in standard textbooks such as Greene, T, and Wuts, P. G. M., Protective Groups in Organic Synthesis, John Wiley & Sons, Inc., New York, N.Y., 1991. Cbz and BOC are commonly used protecting groups in organic synthesis, and their removal conditions are known to those skilled in the art.

Abbreviations Used in the Description of the Preparation of the Compounds of the Present Invention:


	AIBN	2,2′-azobisisobutyronitrile
	BOC	t-butyloxycarbonyl
	BBr₃	boron tribromide
	9-BBN	9-borabicyclo[3.3.1]nonane
	Bn	benzyl
	nBuLi	n-butyl lithium
	Cbz	benzyloxycarbonyl
	CDI	1,1′-carbonyldiimidazole
	MeOTf	methyl trifluoromethanesulfonate
	CH₂Cl₂	dichloromethane
	CH₂I₂	diiodomethane
	(COCl)₂	oxalyl chloride
	Cs₂CO₃	cesium carbonate
	DAST	(diethylamino)sulfur trifluoride
	DMAP	4-(dimethylamino)pyridine
	DMF	N,N-dimethylformamide
	Et	ethyl
	Et₃N	triethylamine
	EtOAc	ethyl acetate
	Et₂Zn	diethylzinc
	H₂O₂	hydrogen peroxide
	Me	methyl
	MeCN	acetonitrile
	MeOH	methanol
	mCPBA	meta-chloroperbenzoic acid
	MS	mass spectrum
	NaBH₄	sodium borohydride
	NaHCO₃	sodium hydrogencarbonate
	NaOAc	sodium acetate
	NBS	N-bromosuccinimide
	Ph	phenyl
	PyBROP	bromotripyrrolidinophosphonium
		hexafluorophosphate
	PPh₃	triphenylphosphine
	Pyr	pyridine
	SOCl₂	thionyl chloride
	TFA	trifluoroacetic acid
	TFFH	N,N,N′,N′-tetramethylformamidinium
		hexafluorophosphate
	THF	tetrahydrofuran
	TLC	thin-layer chromatography
	TsOH	p-toluenesulfonic acid

Reaction Schemes 1-5 illustrate the methods employed in the synthesis of the compounds of the present invention of structural formula I. All substituents are as defined above unless indicated otherwise.
Reaction Scheme 1 illustrates a key step in the synthesis of the novel compounds of structural formula I of the present invention. As shown in reaction Scheme 1, a secondary amide (1-1) (N-Me or N-Et preferred) can be methylated by heating with neat methyl triflate in order to provide an iminoether (1-2). Alternatively other methylating reagents such as methyl iodide or methyl sulfate may be used neat or in a non-nucleophilic organic solvent. As shown in Scheme 1, a bicyclo[2.2.2]octane-1-carboxylic acid (1-3) is converted to an acyl hydrazide (1-4) by using the coupling reagent TFFH and hydrazine in the presence of a tertiary amine base such as triethylamine. Alternatively, other coupling reagents commonly used for preparing amides may be used for this transformation along with hydrazine. Alternatively, a bicyclo[2.2.2]octane-1-carboxylic ester can be heated with hydrazine to prepare acyl hydrazides (1-4). The acyl hydrazide (1-4) and iminoether (1-2) thus produced can be heated together in an inert high boiling organic solvent such as toluene in the presence of a tertiary amine base such as triethylamine to provide bicyclo[2.2.2]octyltriazoles (1-5) of structural formula I.
Alternatively, the reaction can be conducted in the inverse manner as described by reaction Scheme 2. In this procedure a secondary amide (2-1) is prepared from a bicyclo[2.2.2]octane-1-carboxylic acid using a standard peptide coupling reaction. This compound is methylated to form the iminoether (2-2) and reacted with an acyl hydrazide as described for reaction Scheme 1 to provide bicyclo[2.2.2]octyltriazoles (2-3) of structural formula I.
Reaction Scheme 3 describes an alternate approach to compounds of the present invention of structural formula I, in which the key step is the palladium catalyzed Suzuki coupling reaction between a bicyclo[2.2.2]octylbromotriazole (3-1) and an aryl boronic acid to produce triazoles (3-2) of structural formula I. The preferred conditions use tetrakis(triphenylphosphine)palladium(0) as the catalyst in DMF solvent with cesium carbonate, but other catalysts and conditions may be employed, as recognized by those skilled in the art.
Reaction Scheme 4 describes yet another synthetic approach to the formation of compounds of structural formula I. Using this procedure, 4-(bicyclo[2.2.2]octyl)oxadiazoles (4-1) are dehydratively condensed with methylamine, either neat in a melt with methylammonium trifluoroacetate or in buffered MeOH solution. These reactions are best performed at high temperatures in a high pressure reactor to prevent the loss of methylamine.
Reaction Scheme 5 describes yet another synthetic approach to the formation of compounds of structural formula I. Using this procedure, bicyclo[2.2.2]octylcarboxamides (5-1) are converted to iminochlorides (5-2), using a reagent such as oxalyl chloride, thionyl chloride, phosphorus oxychloride or phosphorus pentachloride, optionally in the presence of DMF. The iminochloride (5-2) is condensed with an aryl tetrazole in a high boiling inert organic solvent such as toluene to provide the triazole (5-3).

Preparation of [2.2.2]Bicyclooctyl Intermediates:

The procedures used in the preparation of [2.2.2]bicyclooctyl intermediates for use in the preparation of compounds of the present invention are provided below.
Intermediate Schemes 1-4 describe the preparation of oxadiazoles, which are important intermediates for the synthesis of compounds of structural formula I. They can be converted into compounds of structural formula I using, for example, the reactions described in reaction Scheme 4.
Intermediate Scheme 1 shows a preferred method for the preparation of oxadiazoles via the dehydration of diacyl hydrazides using a dehydrating reagent such as thionyl chloride. Alternatively, other dehydrating reagents such as phosphorus oxychloride, phosphorus pentachloride or oxalyl chloride may be employed. The diacyl hydrazides may be prepared preferentially from a hydrazide and an activated acid, such as an acid chloride, in the presence of a tertiary amine base. Alternatively, standard peptide coupling reactions may be employed to prepare the diacyl hydrazide from a hydrazide and a carboxylic acid.
Intermediate Scheme 2 shows a useful reagent for the dehydration of diacyl hydrazides to oxadiazoles, namely, 2-chloro-1,3-dimethyl-4,5-dihydro-1H-imidazol-3-ium chloride. This reagent in a non-polar solvent (methylene chloride is preferred) along with a tertiary amine base (triethylamine is preferred) gives the desired oxadiazole intermediates in an efficient manner.
Intermediate Scheme 3 shows a preferred reagent for the one pot formation (from a hydrazide and a carboxylic acid) and dehydration of diacyl hydrazides to oxadiazoles: 2-chloro-1,3-dimethyl-4,5-dihydro-1H-imidazol-3-ium chloride. This reagent in a non-polar solvent (methylene chloride is preferred) along with a tertiary amine base (triethylamine is preferred) gives the desired oxadiazole intermediates in an efficient manner.
Intermediate Scheme 4 shows an efficient method for the formation of oxadiazoles from secondary amides and hydrazides. The secondary amide (N-Me or N-Et preferred) can be methylated by heating with neat methyl triflate in order to provide an iminoether. Alternatively other methylating reagents such as methyl iodide or methyl sulfate may be used neat or in a non-nucleophilic organic solvent. Heating the iminoether thus formed in a high boiling inert organic solvent in the presence of a hydrazide affords oxadiazoles as shown in the Scheme.
Intermediate Scheme 5 shows a preferred method for the synthesis of bicyclo[2.2.2]octane-1-carboxylic acid.
Intermediate Schemes 6 and 7 show preferred methods for the preparation of bicyclo[2.2.2]octane-1-carboxylic acids with a heteroaryl group at the R³position as given by structural formula I. Oxadiazoles at the R³position may be prepared by the condensation of a bicyclo[2.2.2]octyl-1-carboxylic acid with an amidoxime as shown in Intermediate Scheme 6. A useful reagent for this coupling is CDI. Alternatively, other reagents useful for dehydration or peptide coupling reactions may be employed. Intermediate Scheme 7 illustrates a preferred method for the synthesis of an intermediate of compounds of structural formula I bearing a thiazole group at the R³position.
Intermediate Schemes 8-14 show preferred methods for the preparation of bicyclo[2.2.2]octane-1-carboxylic acids intermediates in the synthesis of compounds of structural formula I with various alkyl or alkenyl or substituted alkyl groups at the R³position. A key reaction is the Wittig reaction performed on a bicyclo[2.2.2]octane-1-carboxaldehyde, as shown in Intermediate Scheme 8. The double bond in the product of this reaction may be hydrogenated to generate an alkyl group of varying length and character (which will become the R³substituent in structural formula I), depending on the Wittig reagent, as shown in Intermediate Scheme 9. Alternatively, the double bond can be used to introduce other functionality, such as the hydroxy or fluoro group, as shown in Intermediate Scheme 10. The aldehyde itself may be used to provide the difluoromethyl group at position R³, as shown in Intermediate Scheme 11. The alkene product of the Wittig reaction can undergo numerous other transformations, for example, cyclopropanation, as illustrated in Intermediate Scheme 12. Alternatively, the Wittig reagent may contain a remote functional group, for example, a ketal, as illustrated in Intermediate Scheme 13. This functional group may undergo characteristic functional group transformations after the Wittig/reduction sequence, for example, the hydrolysis of a ketal to a ketone, as illustrated in Intermediate Scheme 13, or the reduction of a ketal to an alcohol as illustrated in Intermediate Scheme 14. In this manner compounds of structural formula I with a variety of different R³substituents may be obtained. The specific examples given are intended to convey general principles and are not intended to limit the scope of the R³substituents.
General functional group chemical transformations used to prepare compounds of the present invention are illustrated below in the preparation of specific compounds of the present invention.
These functional group transformations are of a general variety well understood by those skilled in the art.
The following Examples are provided to illustrate the invention and are not to be construed as limiting the scope of the invention in any manner.

EXAMPLE 1

3-Methoxy-4-[4-methyl-5-(4-pentylbicyclo[2.2.2]oct-1-yl)-4H-1,2,4-triazol-3-yl]phenol (1-F)

Step A:

To a magnetically stirred solution of 4-benzyloxy-2-hydroxybenzonitrile (1-A, WO 00/69841) (7.95 g, 35.3 mmol) and iodomethane (5.43 mL, 87.2 mmol) in DMF (90 mL) cooled to −5° was added all at once sodium hydride (60% dispersion, 2.17 g, 54.2 mmol). The mixture was stirred for 30 min, warmed to room temperature and stirred for an additional 2 h. Most of the DMF was removed in vacuo, and the residue was partitioned between water and ethyl acetate. The aqueous phase was extracted three times with ethyl acetate. The combined organic phases were washed with water and saturated brine and dried (MgSO₄). The residue after removal of the solvent in vacuo was triturated with hexane and chromatographed on silica gel with hexanes-CH₂Cl₂(2:3) to give 4-benzyloxy-2-methoxybenzonitrile (1-B). MS: m/z 240 (M+1); ¹H NMR (500 MHz, CDCl₃): δ 7.47 (d, 1H, J=8.4 Hz), 7.36-7.45 (m, 5H), 6.58 (dd, 1H, J=2.3, 8.4 Hz), 6.57 (d, 1H, J=2.3 Hz), 5.10 (s, 2H), 3.88 (s, 3H) ppm.

Step B:

A vigorously stirred suspension of 4-benzyloxy-2-methoxybenzonitrile (1-B) (1.20 g, 5.0 mmol), sodium azide (732 mg, 11.3 mmol), and triethylamine hydrochloride (1.54 g, 11.3 mmol) in toluene (6 mL) was heated at 110° for 48 h. The brown suspension was cooled, water (15 mL) was added, and the mixture stirred for 30 min. The organic layer was separated and extracted with water (5 mL). The combined aqueous extracts were acidified to about pH 1 with concentrated HC1. The gum that initially precipitated solidified upon stirring for 30 min. The solid was filtered, washed with water, and dried to give 5-[4-(benzyloxy)-2-methoxyphenyl]-2H-tetrazole (1-C). ¹H NMR (500 MHz, CDCl₃): δ12.9 (vbs, 1H), 7.37 (d, 1H, J=8.7 Hz), 7.34-7.48 (m, 5H), 6.78 (dd, 1H, J=2.3, 8.7 Hz), 6.70 (d, 1H, J=2.3 Hz), 5.15 (s, 2H), 4.05 (s, 3H) ppm.

Step C:

Oxalyl chloride (3.49 ml, 40 mmol) was added dropwise to a solution of N-methyl-4-pentylbicyclo[2.2.2]octane-1-carboxamide (1-D) (952 mg, 4.0 mmol) in dry CH₂Cl₂at room temperature. After the vigorous gas evolution subsided, the solution was stirred at room temperature for 2 h. The CH₂Cl₂was removed carefully in vacuo at room temperature and then at 50°. The clear syrupy residue was dissolved in toluene (8 mL) and 5-[4-(benzyloxy)-2-methoxyphenyl]-2H-tetrazole (1-C) (1.13 g, 4.0 mmol) added. The mixture was heated at 120° for 9 h. The mixture was cooled, and the precipitated solid was filtered, washed with toluene and dried to afford the triazole hydrochloride salt. The salt was partitioned between CH₂Cl₂and 10% aqueous K₂CO₃. The aqueous phase was extracted twice with CH₂Cl₂. The combined CH₂Cl₂extracts were dried (MgSO₄) and evaporated in vacuo. The residue was chromatographed on silica gel with 5% MeOH/CH₂Cl₂to give 3-[4-(benzyloxy)-2-methoxyphenyl]-4-methyl-5-(4-pentylbicyclo[2.2.2]oct-1-yl)-4H-1,2,4-triazole (1-E). MS: m/z 474 (M+1); ¹H NMR (500 MHz, CDCl₃): δ 7.33-7.47 (m, 6H), 6.65 (dd, 1H, J=2.3, 8.5 Hz), 6.60 (d, 1H, J=2.3 Hz), 5.10 (s, 2H), 3.75 (s, 3H), 3.48 (s, 3H), 2.08 (m, 6H), 1.51 (m, 6H), 1.00-1.35 (m, 8H), 0.89 (t, 3H, J=7.2) ppm.

Step D:

A solution of 3-[4-(benzyloxy)-2-methoxyphenyl]-4-methyl-5-(4-pentylbicyclo[2.2.2]oct-1-yl)-4H-1,2,4-triazole (1-E) (272 mg, 0.572 mmol) in MeOH (8 mL) was hydrogenated for 19 h with 10% Pd/C catalyst (27 mg) at room temperature and atmospheric pressure. The catalyst was filtered and washed with MeOH. The MeOH was removed in vacuo to afford 3-methoxy-4-[4-methyl-5-(4-pentylbicyclo[2.2.2]oct-1-yl)-4H-1,2,4-triazol-3-yl]phenol (1-F). MS: m/z 384 (M+1); ¹H NMR (500 MHz, DMSO-d₆): δ 9.94 (s, 1H), 7.09 (d, 1H, J=8.3), 6.53 (d, 1H, J=1.6 Hz), 6.46 (dd, 1H, J=2.2, 8.2 Hz), 3.72 (s, 3H), 3.40 (s, 3H), 1.95 (m, 6H), 1.44 (m, 6H), 1.07-1.33 (m, 8H), 0.86 (t, 3H, J=7.2).

EXAMPLE 2

3-Methyl-4-[4-methyl-5-(4-pentylbicyclo[2.2.2]oct-1-yl)-4H-1,2,4-triazol-3-yl]phenol (2-G)

Step A:

(Trimethylsilyl) diazomethane (2M/hexane, 53 mL, 106 mmol) was added slowly to a solution of 4-pentylbicyclo[2.2.2]octane-1-carboxylic acid (2-A) (20.3 g, 90.6 mmol) in methylene chloride (100 mL) and methanol (40 mL) until the yellow color persisted. After stirring for 10 min at room temperature, the solution was concentrated in vacuo to give methyl 4-pentylbicyclo[2.2.2]octane-1-carboxylate (2-B). ¹H NMR (500 MHz, CDCl₃): δ 0.89 (t, 3H); 1.20 (m, 8H); 1.39 (m, 6H); 1.77 (m, 6H); 3.65 (s, 3H) ppm.

Step B:

Hydrazine (anhydrous, 103 mL, 88.7 mmol) was added to a solution of methyl 4-pentylbicyclo[2.2.2]octane-1-carboxylate (2-B) in ethylene glycol (180 mL) and the mixture was stirred under reflux for 17 h. After cooling to room temperature, the mixture was poured into water (1500 mL) and extracted with methylene chloride (3×600 mL). The combined extracts were washed twice with water, brine, dried (MgSO₄) and concentrated in vacuo to provide 4-pentylbicyclo[2.2.2]octane-1-carbohydrazide (2-C). ¹H NMR (500 MHz, CDCl₃): δ 0.90 (t, 3H); 1.21 (m, 8H); 1.43 (m, 6H); 1.74 (m, 6H); 3.85 (broad s, 2H); 6.81 (broad s, 1H) ppm.

Step C:

2-Chloro-1,3-dimethyl-4,5-dihydro-1H-imidazol-3-ium chloride (5.07 g, 30.0 mmol) was added to a solution of 2-methyl-4-methoxybenzoic acid (2-D) (856 mg, 5.0 mmol) and 4-pentylbicyclo[2.2.2]octane-1-carbohydrazide (2-C) (1.25 g, 5.25 mmol) in methylene chloride (60 mL) followed by triethylamine (8.36 mL, 60 mmol) and the mixture stirred at room temperature for 48 h. The mixture was diluted with methylene chloride and washed with water, 1N HCl, 10% NaHCO₃, brine, dried (MgSO₄) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, hexane:ethyl acetate, 9:1) to give 2-(4-methoxy-2-methylphenyl)-5-(4-pentylbicyclo[2.2.2]oct-1-yl)-1,3,4-oxadiazole (2-E) Mass spectrum: 369 (M+1); ¹H NMR (500 MHz, CDCl₃): δ 0.93 (t, 3H); 1.27 (m, 8H); 1.56 (m, 6H); 2.03 (m, 6H); 2.70 (s, 3H); 3.89 (s, 3H); 6.86 (m, 2H); 7.89 (d, 1H) ppm.

Step D:

2-(4-Methoxy-2-methylphenyl)-5-(4-pentylbicyclo[2.2.2]oct-1-yl)-1,3,4-oxadiazole (2-E) (988 mg, 2.68 mmol), methylammonium trifluoroacetate (9.72 g, 67 mmol, prepared by combining equimolar amounts of methylamine and trifluoroacetic acid in ether followed by concentration in vacuo), and methylamine (2M/MeOH, 33 mL, 67 mmol) were stirred together in a glass bomb at 150° C. for 114 h. The mixture was concentrated in vacuo and the residue partitioned with methylene chloride and water. The aqueous phase was extracted with methylene chloride and the combined extracts washed with brine, dried (MgSO₄) and concentrated in vacuo. The residue was purified by column chromatography (silica gel, ethyl acetate:hexane, 7:3, then 9:1) to give 3-(4-methoxy-2-methylphenyl)-4-methyl-5-(4-pentylbicyclo[2.2.2]oct-1-yl)-4H-1,2,4-triazole (2-F). Mass spectrum: 382 (M+1); ¹H NMR (500 MHz, CDCl₃): δ 0.93 (t, 3H); 1.27 (m, 8H); 1.56 (m, 6H); 2.12 (m, 6H); 2.18 (s, 3H); 3.49 (s, 3H); 3.87 (s, 3H); 6.85 (m, 2H); 7.24 (d, 1H) ppm.

Step E:

Boron tribromide (1M/CH₂Cl₂, 3.21 mL, 3.21 mmol) was added to a solution of 3-(4-methoxy-2-methylphenyl)-4-methyl-5-(4-pentylbicyclo[2.2.2]oct-1-yl)-4H-1,2,4-triazole (2-F) (410 mg, 1.07 mmol) in methylene chloride (6 mL) at 0° C. The mixture was stirred at room temperature for 2 h. The solution was washed with water, 10% NaHCO₃, dried (MgSO₄) and concentrated in vacuo. The residue was purified by preparative TLC (silica gel, MeOH:methylene chloride, 5:95) to provide 3-methyl-4-[4-methyl-5-(4-pentylbicyclo[2.2.2]oct-1-yl)-4H-1,2,4-triazol-3-yl]phenol (2-G). Mass spectrum: 393 (M+1); ¹H NMR (500 MHz, CDCl₃): δ 0.93 (t, 3H); 1.27 (m, 8H); 1.56 (m, 6H); 1.97 (s 3H); 2.12 (m, 6H); 3.50 (s, 3H); 6.65 (m, 2H); 6.98 (d, 1H) ppm.

EXAMPLE 3

3-Chloro-4-[4-methyl-5-(4-pentylbicyclo[2.2.2]oct-1-yl)-4H-1,2,4-triazol-3-yl]phenol (3-G)

Step A:

Oxalyl chloride (505 μL, 5.79 mmol) was added dropwise to a mixture of 4-pentylbicyclo[2.2.2]octane-1-carboxylic acid (3-A) in methylene chloride (10 mL). The solution was stirred at room temperature for 3 h and then concentrated in vacuo to give 4-pentylbicyclo[2.2.2]octane-1-carbonyl chloride (3-B). ¹H NMR (500 MHz, CDCl₃): δ 0.90 (t, 3H); 1.21 (m, 8H); 1.45 (m, 6H); 1.88 (m, 6H) ppm.

Step B:

N,N-Diisopropylethylamine (1.44 mL, 11.1 mmol) was added to a mixture of 4-pentylbicyclo[2.2.2]octane-1-carboxylic acid (3-A) (1.09 g, 4.45 mmol) and methylamine hydrochloride (1.5 g, 22.3 mmol) in methylene chloride (10 mL) was added and the mixture stirred at room temperature for 18 h. After diluting with methylene chloride, the mixture was washed with water, brine, dried (MgSO₄) and concentrated in vacuo to give N-methyl-4-pentylbicyclo[2.2.2]octane-1-carboxamide (3-C). ¹H NMR (500 MHz, CDCl₃): δ 0.91 (t, 3H); 1.22 (m, 8H); 1.43 (m, 6H); 1.77 (m, 6H); 2.82 (d, 3H) ppm.

Step C:

Oxalyl chloride (846 μL, 9.7 mmol) was added dropwise to a solution of N-methyl-4-pentylbicyclo[2.2.2]octane-1-carboxamide (3-C) (230 mg, 0.97 mmol) in methylene chloride (2.0 mL) and the mixture stirred at room temperature for 4 h. The solvent and excess reagent were removed in vacuo to provide N-methyl-4-pentylbicyclo[2.2.2]octane-1-carboximidoyl chloride (3-D). Toluene (1.5 mL) was added followed by 5-(2-chloro-4-methoxyphenyl)-1H-tetrazole (3-E) (204 mg, 0.97 mmol) and the mixture refluxed for 18 h. The reaction was cooled to room temperature and the precipitate was filtered, washed with cold toluene, hexane, dissolved in methylene chloride, dried (MgSO₄) and concentrated in vacuo to provide 3-(2-chloro-4-methoxyphenyl)-4-methyl-5-(4-pentylbicyclo[2.2.2]oct-1-yl)-4H-1,2,4-triazole (3-F). Mass spectrum: 402 (M+1); ¹H NMR (500 MHz, CDCl₃): δ 0.94 (t, 3H); 1.27 (m, 8H); 1.56 (m, 6H); 2.13 (m, 6H); 3.56 (s, 3H); 3.89 (s, 3H); 6.95 (dd, 1H); 7.07 (d, 1H); 7.43 (d, 1H).

Step D:

Boron tribromide (135 μL, 1.43 mmol) was added dropwise to a solution of 3-(2-chloro-4-methoxyphenyl)-4-methyl-5-(4-pentylbicyclo[2.2.2]oct-1-yl)-4H-1,2,4-triazole (3-F) (287 mg, 0.714 mmol) in methylene chloride (5 mL) at 0° C. The mixture was stirred at room temperature for 2.5 h. The solution was washed with water, 10% NaHCO₃, dried (MgSO₄) and concentrated in vacuo and the residue purified by column chromatography (silica gel, 5% MeOH/methylene chloride) to provide 3-chloro-4-[4-methyl-5-(4-pentylbicyclo[2.2.2]oct-1-yl)-4H-1,2,4-triazol-3-yl]phenol (3-G). Mass spectrum: 388 (M+1); ¹H NMR (500 MHz, CDCl₃): δ 0.93 (t, 3H); 1.26 (m, 8H); 1.56 (m, 6H); 2.13 (m, 6H); 3.58 (s, 3H); 6.69 (dd, 1H); 6.92 (d, 1H); 7.09 (d 1H) ppm.

EXAMPLE 4

5-(4-{1-Methyl-5-[2-(trifluoromethyl)phenyl]-1-H-1,2,4-triazol-3-yl}bicyclo[2.2.2]oct-1-yl)pentan-2-ol (4-J)

Step A:

[2-(2-Methyl-1,3-dioxolan-2-yl)ethyl](triphenyl)phosphonium bromide (4-A, Synthesis: 532 (1986)) (5.99 g, 12.7 mmol) was stirred in dry THF (200 mL). Potassium bis(trimethylsilyl)amide (20.4 mL, 2M soln in toluene, 10.2 mmol) was added. The reaction was allowed to stir for 30 min. The reaction mixture was then cooled to −78° C. Methyl 4-formylbicyclo[2.2.2]octane-1-carboxylate was added at −78° C. by cannula. The reaction was allowed to warm to room temperature overnight. The volume was reduced by evaporation of THF in vacuo. 100 mL of water was added. The mixture was then layered with 100 mL of diethyl ether. The ether was extracted and dried (MgSO₄). The product (methyl 4-[(1E)-3-(2-methyl-1,3-dioxolan-2-yl)prop-1-enyl]bicyclo [2.2.2]octane-1-carboxylate (4-B)) was purified by flash chromatography on silica gel with 10/90 ethyl acetate-hexane mixture.

Step B:

Methyl 4-[(1E)-3-(2-methyl-1,3-dioxolan-2-yl)prop-1-enyl]bicyclo [2.2.2]octane-1-carboxylate (4-B) (1.1 g) was stirred in ethanol (75 mL). A spatula tip scoop of 10% Pd on carbon (150 mg) was added. A hydrogen balloon was added and the mixture was stirred under hydrogen atmosphere for 3 h. The palladium-on-carbon was filtered and the ethanol was removed in vacuo to yield methyl 4-[3-(2-methyl-1,3-dioxolan-2-yl)propyl]bicyclo[2.2.2]octane-1-carboxylate (4-C).

Step C:

Methyl 4-[3-(2-methyl-1,3-dioxolan-2-yl)propyl]bicyclo [2.2.2]octane-1-carboxylate (4-C) (1.0 g, 3.38 mmol) was stirred in a solution of 90% methanol and 10% water (50 mL). Excess potassium hydroxide (2.0 g) was added. The mixture was refluxed overnight. The cooled mixture was acidified with 1N hydrochloric acid (100 mL) and then washed twice with ethyl acetate (100 mL). The combined organic layers were dried (MgSO₄). Ethyl acetate was removed in vacuo yielding pure 4-[3-(2-methyl-1,3-dioxolan-2-yl)propyl]bicyclo[2.2.2]octane-1-carboxylic acid (4-D).

Step D:

4-[3-(2-Methyl-1,3-dioxolan-2-yl)propyl]bicyclo [2.2.2]octane-1-carboxylic acid (4-D) (0.200 g, 0.708 mmol) was combined with 2-(trifluoromethyl)benzoic hydrazide (4-E) (0.173 g, 0.847 mmol) and azeotroped twice from toluene. The mixture was then stirred in dry methylene chloride (10 mL). 2-Chloro-1,3-dimethylimidazolinium chloride (4-F) (0.718 g, 4.25 mmol) was added followed by 1.184 mL of triethylamine. The reaction was allowed to stir for 2 h. The reaction was diluted with methylene chloride and was washed with water. The resulting oxadiazole, 2-{4-[3-(2-methyl-1,3-dioxolan-2-yl)propyl]bicyclo [2.2.2]oct-1-yl}-5-[2-(trifluoromethyl)phenyl]-1,3,4-oxadiazole (4-G) was purified by flash chromatography on silica gel with 50/50 ethyl acetate-hexane mixture.

Step E:

2-{4-[3-(2-Methyl-1,3-dioxolan-2-yl)propyl]bicyclo [2.2.2]oct-1-yl}-5-[2-(trifluoromethyl)phenyl]-1,3,4-oxadiazole (4-G) (0.158 g) was stirred in a mixture of 90% acetone/10% water (20 mL). p-Toluenesulfonic acid (10 mg) was added to the solution. The reaction was heated to reflux for 1 h. The volume was reduced by evaporation of acetone in vacuo. The mixture was then layered with ethyl acetate (25 mL) and saturated sodium bicarbonate solution (25 mL). The ethyl acetate layer was extracted and dried (MgSO₄). Solvent was removed in vacuo to afford pure 5-(4-{5-[2-(trifluoromethyl)phenyl]-1,3,4-oxadiazol-2-yl}bicyclo[2.2.2]oct-1-yl)pentan-2-one (4-H).

Step F:

5-(4-{5-[2-(Trifluoromethyl)phenyl]-1,3,4-oxadiazol-2-yl}bicyclo [2.2.2]oct-1-yl)pentan-2-one (4-H) (0.072 g) was stirred in methanol (2 mL) at 0° C. Sodium borohydride (20 mg) was added. The reaction was allowed to stir to room temperature. The mixture was then layered with ethyl acetate (15 mL) and water (15 mL). The ethyl acetate layer was extracted and dried (MgSO₄). Solvent was removed in vacuo to afford pure 5-(4-{5-[2-(trifluoromethyl)phenyl]-1,3,4-oxadiazol-2-yl}bicyclo[2.2.2]oct-1-yl)pentan-2-ol (4-I).

Step G:

5-(4-{5-[2-(Trifluoromethyl)phenyl]-1,3,4-oxadiazol-2-yl}bicyclo [2.2.2]oct-1-yl)pentan-2-ol (4-I) (50 mg) was placed in a sealed vial in a solution of 2M methylamine in methanol (2.5 mL). A small spatula scoop of methylamine TFA salt was added and the vial was sealed. The sealed vial was heated to 150° C. for 3 d. The reaction was diluted with ethyl acetate (15 mL), washed with water (15 mL), and dried (MgSO₄). Ethyl acetate was removed in vacuo. The product, 5-(4-{1-methyl-5-[2-(trifluoromethyl)phenyl]-1-H-1,2,4-triazol-3-yl}bicyclo [2.2.2]oct-1-yl)pentan-2-ol (4-J), was purified by preparative reverse phase HPLC on a C-18 silica gel column using a gradient of acetonitrile-water buffered with 0.1% trifluoroacetic acid. The effluent containing the pure triazole was made basic with 10% NaHCO₃, evaporated in vacuo to remove most of the acetonitrile, and extracted with methylene chloride. The organic extract was dried (MgSO₄) and evaporated, and the residue dried under vacuum to provide the desired compound. MS (ESI⁺)=422.5 (M+1); ¹H NMR (500 MHz, CDCl₃): δ 1.21 (2H, m), 1.23 (3H, d, J=6.5 Hz), 1.29 (2H, m), 1.57 (6H, m), 2.13 (6H, m), 3.47 (3H, s), 3.85 (1H, m), 7.51 (1H, m), 7.70 (2H, m), 7.85 (1H, m) ppm.

EXAMPLE 5

3-Chloro-4-{5-[4-(4-hydroxypentyl)bicyclo[2.2.2]oct-1-yl]-1-methyl-1-H-1,2,4-triazol-3-yl}phenol (5-K)

Step A:

4-[3-(2-Methyl-1,3-dioxolan-2-yl)propyl]bicyclo [2.2.2]octane-1-carboxylic acid (4-D) (0.300 g, 1.06 mmol) was combined with 2-chloro-4-methoxybenzohydrazide (5-E) (0.255 g, 1.275 mmol) and azeotroped twice from toluene. The mixture was then stirred in dry methylene chloride (15 mL). 2-Chloro-1,3-dimethylimidazolinium chloride (5-F) (1.075 g, 6.36 mmol) was added followed by 1.77 mL of triethylamine. The reaction was allowed to stir for 2 h. The reaction was diluted with methylene chloride and was washed with water. The resulting oxadiazole, 2-(2-chloro-4-methoxyphenyl)-5-{4-[3-(2-methyl-1,3-dioxolan-2-yl)propyl]bicyclo [2.2.2]oct-1-yl}-1,3,4-oxadiazole (5-G) was purified by flash chromatography on silica gel with 50/50 ethyl acetate-hexane mixture.

Step B:

2-(2-Chloro-4-methoxyphenyl)-5-{4-[3-(2-methyl-1,3-dioxolan-2-yl)propyl]bicyclo[2.2.2]oct-1-yl}-1,3,4-oxadiazole (5-G) (0.200 g) was stirred in a mixture of 90% acetone/10% water (20 mL). p-Toluenesulfonic acid (15 mg) was added to the solution. The reaction was heated to reflux for 1 h. The volume was reduced by evaporation of acetone in vacuo. The mixture was then layered with ethyl acetate (25 mL) and saturated sodium bicarbonate solution (25 mL). The ethyl acetate layer was extracted and dried (MgSO₄). Solvent was removed in vacuo to afford pure 5-{4-[5-(2-chloro-4-methoxyphenyl)-1,3,4-oxadiazol-2-yl]bicyclo[2.2.2]oct-1-yl}pentan-2-one (5-H).

Step C:

5-{4-[5-(2-Chloro-4-methoxyphenyl)-1,3,4-oxadiazol-2-yl]bicyclo [2.2.2]oct-1-yl}pentan-2-one (5-H) (0.150 g, 0.373 mmol) was stirred in methanol (5 mL) at 0° C. Sodium borohydride (0.0169 g, 0.448 mmol) was added. The reaction was allowed to stir to room temperature. The mixture was then layered with ethyl acetate (20 mL) and water (20 mL). The ethyl acetate layer was extracted and dried (MgSO₄). Solvent was removed in vacuo to afford 5-{4-[5-(2-chloro-4-methoxyphenyl)-1,3,4-oxadiazol-2-yl]bicyclo [2.2.2]oct-1-yl}pentan-2-ol (5-I).

Step D:

5-{4-[5-(2-Chloro-4-methoxyphenyl)-1,3,4-oxadiazol-2-yl]bicyclo [2.2.2]oct-1-yl}pentan-2-ol (5-I) (50 mg) was placed in a sealed vial in a solution of 2M methylamine in methanol (2.5 mL). A small spatula scoop of methylamine TFA salt was added and the vial was sealed. The sealed vial was heated to 150° C. for 24 h. The reaction was diluted with ethyl acetate (15 mL), washed with water (15 mL), and dried (MgSO₄). Ethyl acetate was removed in vacuo. The product, 5-{4-[5-(2-chloro-4-methoxyphenyl)-1-methyl-1-H-1,2,4-triazol-3-yl]bicyclo[2.2.2]oct-1-yl}pentan-2-ol (5-J), was purified by preparative TLC with 5% methanol/95% ethyl acetate.

Step E:

5-{4-[5-(2-Chloro-4-methoxyphenyl)-1-methyl-1-H-1,2,4-triazol-3-yl]bicyclo [2.2.2]oct-1-yl}pentan-2-ol (5-J) (0.036 g, 0.086 mmol) was placed in a small vial with 0.5 mL of DMF. Sodium ethanethiolate (0.0218 g, 0.260 mmol) was added to the solution. The vial was sealed and heated to 100° C. for 1.5 h. The incomplete reaction required another 1.5 equivalents of sodium ethanethiolate (0.011 g). The vial was resealed and heated at 100° C. for 1 h. The product, 3-chloro-4-{5-[4-(4-hydroxypentyl)bicyclo [2.2.2]oct-1-yl]-1-methyl-1-H-1,2,4-triazol-3-yl}phenol (5-K) was purified by preparative reverse phase HPLC on a C-18 silica gel column using a gradient of acetonitrile-water buffered with 0.1% trifluoroacetic acid. The effluent containing the pure triazole was made basic with 10% NaHCO₃, evaporated in vacuo to remove most of the acetonitrile, and extracted with methylene chloride. The organic extract was dried (MgSO₄) and evaporated, and the residue dried under vacuum to provide the desired compound. MS (ESI⁺)=404.4 (M+1).

EXAMPLE 6

5-{4-[5-(2-chloro-4-hydroxyphenyl)-4-methyl-4H-1,2,4-triazol-3-yl]bicyclo[2.2.2]oct-1-yl}pentan-2-one (6-L)

3-Chloro-4-{5-[4-(4-hydroxypentyl)bicyclo [2.2.2]oct-1-yl]-4-methyl-4H-1,2,4-triazol-3-yl}phenol (5-K) (0.0035 g, 0.00869 mmol) was stirred in 0.5 mL of dry methylene chloride over activated 4 A sieves. N-methylmorpholine N-oxide (0.0015 g, 0.013 mmol) was added. The mixture was allowed to stir under N₂for 15 min. Tetrapropylammonium perruthenate (0.00112 g, 0.00956 mmol) was added and the reaction was allowed to stir for 2 h. The mixture was filtered through celite filtering agent. The product, 5-{4-[5-(2-chloro-4-hydroxyphenyl)-4-methyl-4H-1,2,4-triazol-3-yl]bicyclo [2.2.2]oct-1-yl}pentan-2-one (6-L), was purified by preparative reverse phase HPLC on a C-18 silica gel column using a gradient of acetonitrile-water buffered with 0.1% trifluoroacetic acid. The effluent containing the pure triazole was basified with 10% NaHCO₃, evaporated in vacuo to remove most of the acetonitrile, and extracted with methylene chloride. The organic extract was dried (MgSO₄) and evaporated, and the residue dried under vacuum to provide the desired compound. MS (ESI⁺)=402.3 (M+1).

EXAMPLE 7

3-(4-Fluorophenyl)-5-[4-[4-methyl-5-[2-(trifluoromethyl)phenyl]-4H-1,2,4-triazol-3-yl]bicyclo[2.2.2]oct-1-yl]-1,2,4-oxadiazole (7-F)q2

Step A:

To a suspension of 4-(methoxycarbonyl)bicyclo[2.2.2]octane-1-carboxylic acid (7-A) (0.906 g, 4.27 mmol) in dichloromethane (20 mL) was added 1,1′-carbonyldiimidazole (1.04 g, 6.41 mmol). The reaction turned into a clear solution instantly with evolving of gas. After the mixture was stirred at room temperature for 1 h, 4-fluorobenzamidoxime was added (1.98 g, 12.8 mmol). Stirring was continued overnight. The mixture was then concentrated and the residue was refluxed in toluene for 16 h. The mixture was concentrated and the residue was purified by column chromatography using hexane/ethyl acetate as eluent (7/1) to give methyl 4-[3-(4-fluorophenyl)-1,2,4-oxadiazol-5-yl]bicyclo[2.2.2]octane-1-carboxylate acid (7-B) as a white solid. ¹H NMR (500 MHz, CDCl₃) δ 1.96-1.99 (m, 6H), 2.08-2.14 (m, 6H), 3.71 (s, 3H), 7.16-7.20 (m, 2H), 8.08-8.10 (m, 2H) ppm. ESI-MS m/z (M+H) 349.2.

Step B:

The ester (7-B) (1.01 g, 3.06 mmol) was treated with KOH (0.52 g, 9.18 mmol) in methanol/water (95/5, 20 mL). After it was heated at 60° C. for 12 h, the reaction mixture was concentrated, diluted with water, extracted twice with ethyl acetate. The aqueous layer was acidified with 1N HC1 aqueous solution and a white solid precipitated out. The solid 4-[3-(4-fluorophenyl)-1,2,4-oxadiazol-5-yl]bicyclo[2.2.2]octane-1-carboxylic acid (7-C) was collected and further dried by co-evaporating with toluene. ESI-MS m/z (M+H) 317.2.

Step C:

A mixture of the acid (7-C) (138.9 mg, 0.439 mmol) and 2-(trifluoromethyl)benzoic hydrazide (7-D) (89.7 mg, 0.439 mmol) was first co-evaporated with toluene three times. Dichloromethane (7 mL) was added to the mixture as solvent. To the resulting suspension was added 2-chloro-1,3-dimethylimidazolinium chloride (743 mg, 4.39 mmol) followed by triethylamine (1.2 mL, 8.78 mmol). The mixture was allowed to stir at room temperature under nitrogen for 48 h to ensure the completion of the reaction. The reaction mixture was then diluted with dichloromethane, washed with water, 1N HC1, saturated sodium bicarbonate aqueous solution, and lastly brine. The organics were dried over anhydrous sodium sulfate, filtered and concentrated. The residue was purified by column chromatography using hexane/ethyl acetate (3/1) as eluent to give 3-(4-fluorophenyl)-5-(4-{5-[2-(trifluoromethyl)phenyl]-1,3,4-oxadiazol-2-yl}bicyclo [2.2.2]oct-1-yl)-1,2,4-oxadiazole (7-E) as a white solid. ¹H NMR (500 MHz, CDCl₃) δ 2.25 (s, 12H), 7.21 (t, J=8.7 Hz, 2H), 7.74-7.76 (m, 2H), 7.91 (m, 1H), 8.11-8.15 (m, 3H) ppm. ESI-MS m/z (M+H) 485.2.

Step D:

A mixture of above 1,2,4-oxadiazole (7-E) (115.2 mg, 0.238 mmol) and the trifluoroacetic acid salt of methylamine (1.73 g, 11.9 mmol) in a 2M solution of methylamine in methanol (4 mL) was heated at 150° C. in a sealed tube for 48 h. The mixture was then concentrated, and the residue was taken up in dichloromethane, washed with saturated sodium bicarbonate aqueous solution. The organics were concentrated and the residue was purified using reverse-phase HPLC with TFA-buffered acetonitrile/water (40-80%) as eluent. The fractions containing the product were combined, neutralized with saturated sodium bicarbonate aqueous solution and lyophilized from acetonitrile/water to provide 3-(4-fluorophenyl)-5-(4-{4-methyl-5-[2-(trifluoromethyl)phenyl]-4H-1,2,4-triazol-3-yl}bicyclo[2.2.2]oct-1-yl)-1,2,4-oxadiazole (7-F). ¹HNMR (CDCl₃) δ 2.25-2.35 (m, 12H), 3.53 (s, 3H), 7.21 (t, J=8.7 Hz, 2H), 7.54 (m, 1H), 7.73 (m, 2H), 7.88 (m, 1H), 8.13 (m, 2H). ESI-MS m/z (M+H) 498.2.

EXAMPLE 8

4-[4-Methyl-5-(4-phenylbicyclo[2.2.2]oct-1-yl)-4H-1,2,4-triazol-3-yl]-3-(trifluoromethyl)phenol (8-F)

Preparation of 4-phenylbicyclo[2.2.2]octane-1-carboxylic acid (8-A)

LITERATURE REFERENCE

Chapman, N. B, Sotheeswaran, S., and Toyne, K. J, J. Org. Chem, 35: 917-923 (1970)

Step A:

To a magnetically stirred solution of 4-phenylbicyclo[2.2.2]octane-1-carboxylic acid (8-A) (70 mg, 0.30 mmol) in methylene chloride (1 mL) at room temperature was added 2 M oxalyl chloride in methylene chloride (0.61 mL, 1.22 mmol). Two drops of catalytic DMF were added to catalyze the reaction. The reaction was stirred for 30 min and solvent and reagent removed in vacuo. Methylene chloride (1 mL) was added to the residue, followed by 4-(benzyloxy)-2-(trifluoromethyl)benzoic hydrazide (8-B) (141 mg, 0.46 mmol) and triethylamine (0.07 mL, 0.46 mmol). The reaction was stirred at room temperature overnight to afford intermediate 8-C, N′-[4-(benzyloxy)-2-(trifluoromethyl)benzoyl]-4-phenylbicyclo[2.2.2]octane-1-carbohydrazide, which was not isolated. To the crude product (8-C) were then added 2-chloro-1,3-dimethylimidazolinium chloride (257 mg, 1.52 mmol), more triethylamine (0.42 mL, 3.04 mmol), and methylene chloride (2 mL). The reaction was stirred at room temperature for 4 h. The reaction mixture was then diluted with methylene chloride (30 mL) and washed with water (30 mL) two times and with brine (30 mL) once. The combined aqueous layers were extracted with methylene chloride (25 mL) once. The combined organic layers were dried (MgSO₄) and the solvent removed in vacuo. The residue was chromatographed on silica with 10% ethyl acetate in hexanes as eluant to give 2-[4-(benzyloxy)-2-(trifluoromethyl)phenyl]-5-(4-phenylbicyclo[2.2.2]oct-1-yl)-1,3,4-oxadiazole (8-D). MS: m/z 505 (M+1).

Step B:

The trifluoroacetate salt of methylamine (380 mg, 2.61 mmol) and 2-[4-(benzyloxy)-2-(trifluoromethyl)phenyl]-5-(4-phenylbicyclo[2.2.2]oct-1-yl)-1,3,4-oxadiazole (8-D) were suspended in a 2 M solution of methylamine in methanol (1.3 mL, 2.61 mmol) and heated at 150° C. overnight. After being cooled to room temperature, the reaction mixture was partitioned between ethyl acetate (25 mL) and saturated aqueous sodium bicarbonate (30 mL). The layers were separated and the aqueous layer extracted with twice with ethyl acetate (25 mL). The combined organic layers were washed with brine, dried (MgSO₄), and solvent removed in vacuo. The residue was then dissolved in methanol (8 mL) and purified by reverse phase chromatography using gradient elution with 10% acetonitrile (0.1% TFA)/water (0.1% TFA) to 100% acetonitrile (0.1% TFA) over 10 min (20 mL/min). The fractions containing product were partitioned between saturated aqueous sodium bicarbonate (25 mL) and methylene chloride (15 mL). The layers were separated and the aqueous layer was extracted with methylene chloride (15 mL) three times, dried (MgSO₄), and the solvent removed in vacuo to afford 3-[4-(benzyloxy)-2-(trifluoromethyl)phenyl]-4-methyl-5-(4-phenylbicyclo[2.2.2]oct-1-yl)-4H-1,2,4-triazole (8-E). MS: m/z 518 (M+1).

Step C:

The 3-[4-(Benzyloxy)-2-(trifluoromethyl)phenyl]-4-methyl-5-(4-phenylbicyclo[2.2.2]oct-1-yl)-4H-1,2,4-triazole (8-E) (27 mg, 0.05 mmol) was dissolved in ethyl acetate/methanol (1:1, 4 mL) to which 10% palladium-on-carbon (4 mg) was added. The reaction was then placed under hydrogen atmosphere and stirred for 3 h at room temperature and pressure. After appropriate evacuation of the hydrogen atmosphere, the palladium was filtered through a filter aid with methanol (40 mL). The filtrate was collected and the solvent removed in vacuo to afford 4-[4-methyl-5-(4-phenylbicyclo[2.2.2]oct-1-yl)-4H-1,2,4-triazol-3-yl]-3-(trifluoromethyl)phenol (8-F). MS: m/z 428 (M+1); ¹H NMR (500 MHz, CDCl₃): δ 1.92 (6H, m), 2.11 (6H, m), 3.41 (3H, s), 7.17 (2H, m), 7.24 (1H, m), 7.31 (2H, m), 7.38 (3H, m) ppm.

EXAMPLE 9

3-Chloro-4-[5-(4-ethylbicyclo[2.2.2]oct-1-yl)-4-methyl-4H-1,2,4-triazol-3-yl]phenol (9-E)

Step A:

To a stirred solution of 4-ethyl-1-carboxylbicyclo[2.2.2]octane (Chapman, N. B. et al. J. Org. Chem., 1970, 35, 917) (45 mg, 0.26 mmol) in 1 mL of degassed DMF were added methylamine (2M in THF, 1 mL, 2 mmol), triethylamine (0.075 mL, 0.53 mmol) and TFFH (70 mg, 0.26 mmol). The solution was stirred at room temperature for 1 h, then diluted with 20 mL of ethyl acetate and washed with 1N aqueous HCl and brine. The organic layer was dried over anhydrous sodium sulfate and evaporated. The brown oily residue was loaded onto a flash silica gel column and eluted with a gradient ranging from 10 to 40% of ethyl acetate in hexanes. 4-Ethyl-N-methylbicyclo[2.2.2]octane-1-carboxamide (9-B) was isolated as a clear, colorless oil.
¹H NMR (500 MHz, CDCl₃): δ 0.80 (3H, t, J=7.2 Hz), 1.18 (2H, q, J=7.2 Hz), 1.42 (6H, m), 1.76 (6H, m), 2.81 (3H, d, J=6.1 Hz).

Step B:

To a stirred solution of 9-B (45 mg, 0.23 mmol) in 0.25 mL of dry CH₂Cl₂was added oxalyl chloride (2M in CH₂Cl₂, 0.29 mL, 0.58 mmol) and 1 drop of dry DMF. The solution was stirred at room temperature for 2 h, then evaporated. The yellow residue was redissolved in dry toluene and 5-(2-chloro-4-methoxyphenyl)-2H-tetrazole (9-C) was added. The reaction mixture was heated to reflux under inert atmosphere and stirred for additional 1.5 h before being cooled down to room temperature. The solid was filtered, washed with toluene, then redissolved in methylene chloride and washed with saturated aqueous sodium bicarbonate and brine solution. The organic layer was dried, then evaporated. The yellowish residue was purified on a short plug of flash silica gel, eluting with a gradient ranging from 0% to 3% of methanol in methylene chloride. 3-(2-Chloro-4-methoxyphenyl)-5-(4-ethylbicyclo[2.2.2]oct-1-yl)-4-methyl-4H-1,2,4-triazole (9-D) was isolated as a white powder. MS (ESI⁺)=360.3 (M+1); ¹H NMR (500 MHz, CDCl₃): δ 0.82 (3H, t, J=7.0 Hz), 1.22 (2H, q, J=7.0 Hz), 1.52 (6H, m), 2.10 (6H, m), 3.55 (3H, s), 3.88 (3H, s), 6.92 (1H, dd, J=8.4 Hz, J=2.8 Hz), 7.04 (1H, d, J=2.4 Hz), 7.41 (1H, d, J=8.4 Hz).

Step C:

Triazole 9-D (30 mg, 0.08 mmol) was dissolved in 0.5 mL of dry methylene chloride, placed under an inert atmosphere, and cooled to 0° C. To this solution was added BBr₃(1 M in CH₂Cl₂, 0.25 mL, 0.25 mmol) and the cooling bath was immediately removed. The reaction was stirred for 2 h then diluted with 20 mL of methylene chloride and washed with 1 N aqueous NaOH and brine. The residue was chromatographed by reverse-phase HPLC, eluting with a gradient of 0 to 100% acetonitrile in water. The product, 3-chloro-4-[5-(4-ethylbicyclo[2.2.2]oct-1-yl)-4-methyl-4H-1,2,4-triazol-3-yl]phenol (9-E), was isolated as a white powder. MS (ESI⁺)=346.2 (M+1); ¹H NMR (500 MHz, CDCl₃): δ 0.85 (3H, t, J=7.5 Hz), 1.25 (2H, q, J=7.5 Hz), 1.55 (6H, m), 2.13 (6H, m), 3.58 (3H, s), 6.68 (1H, dd, J=8.4 Hz, J=2.6 Hz), 6.91 (1H, d, J=2.6 Hz), 7.10 (1H, d, J=8.4 Hz).

EXAMPLE 10

3-{4-[2-(Ethylsulfonyl)ethyl]bicyclo[2.2.2]oct-1-yl}-4-methyl-5-[2-(trifluoromethyl)phenyl]-4H-1,2,4-triazole (10-6)

Step A:

Diethyl (ethylsulfonomethane)phosphonate (1.12 g, 4.6 mmol) (Popoff, I. C. et al. J. Org. Chem. 34: 1128-30 (1969)) and 4-carbomethoxybicyclo[2.2.2]octane-1-carboxaldehyde (10-1) (0.82 g, 4.2 mmol) (Adcock, W., Kok, G. B. J. Org. Chem. 50: 1079-1087 (1985)) were dissolved in 8 mL of absolute methanol. The mixture was placed under nitrogen atmosphere, cooled in an ice-bath, and treated with 0.5M solution of sodium methoxide in methanol (8.8 mL, 4.4 mmol). The reaction mixture was kept under reflux for 4 h, then cooled to room temperature, concentrated under diminished pressure, then treated with 2 mL of water and allowed to sit in the refrigerator overnight. The mixture was filtered and the solid washed with a small amount of cold 1:1 MeOH/water. The resulting white solid was collected and dried under vacuum to give the unsaturated sulfone 10-2. MS (ESI⁺)=287 (M+1).

Step B:

Sulfone 10-2 (880 mg, 3.08 mmol) was dissolved in a 1:2 mixture of ethyl acetate/methanol (30 mL), placed under nitrogen atmosphere, then treated with 10% Pd/C (800 mg). The reaction was placed under hydrogen atmosphere and stirred vigorously for 90 min. The resulting solution was filtered through celite, washed with methanol and ethyl acetate and evaporated to give methyl 4-[2-(ethylsulfonyl)ethyl]bicyclo[2.2.2]octane-1-carboxylate (10-3) as a white solid.

Step C:

Ester 10-3 (880 mg, 3 mmol) was dissolved in 10% water/methanol solution (100 mL) and treated with 1 g of potassium hydroxide. The reaction was heated at 60° C. for 1 h then at 45° C. overnight. The mixture was concentrated in vacuo then acidified to pH 2 with 1M HCl and extracted with three portions of methylene chloride. The organic layers were combined, dried over anhydrous sodium sulfate and evaporated to give 4-[2-(ethylsulfonyl)ethyl]bicyclo[2.2.2]octane-1-carboxylic acid (10-4).

Step D:

Carboxylic acid 10-4 (810 mg, 2.96 mmol) was dissolved in 12 mL of anhydrous methylene chloride under nitrogen atmosphere, treated with oxalyl chloride (2M in methylene chloride, 4.4 mL, 8.8 mmol) and subsequently with 5 drops of DMF. The reaction was stirred at room temperature under nitrogen atmosphere for 90 min, then evaporated and placed under vacuum for 20 min. The acid chloride was dissolved in anhydrous methylene chloride (12 mL), cooled in an ice-bath, and then treated dropwise with a solution of methylamine (2M in THF, 8.9 mL, 17.8 mmol). Upon addition of the amine, the cooling bath was removed and the reaction stirred at ambient temperature for 30 min. The mixture was diluted with 200 mL of methylene chloride and washed with 1N aqueous HCl, saturated aqueous sodium bicarbonate, and brine. The organic layer was dried over anhydrous sodium sulfate and evaporated. The residue was subjected to chromatography on silica gel eluting with a gradient from 0 to 3.5% methanol in methylene chloride to give 4-[2-(ethylsulfonyl)ethyl]-N-methylbicyclo[2.2.2]octane-1-carboxamide 10-5 as a white powder. MS (ESI⁺)=288 (M+1).

Step E:

Methyl amide 10-5 (220 mg, 0.77 mmol) was dissolved in anhydrous methylene chloride (2 mL) and treated with oxalyl chloride (2M in methylene chloride, 0.77 mL, 1.54 mmol) and DMF (2 drops). The solution was stirred at room temperature for 1 h, then solvent removed by evaporation under diminished pressure. The residue was redissolved in anhydrous toluene (2 mL) and treated with 5 [2-(trifluoromethyl)phenyl]1H-tetrazole (214 mg, 1 mmol). The mixture was refluxed for 18 h. The reaction was cooled to room temperature and the cream-colored precipitate was filtered and washed to give 300 mg of crude product as the HCl salt. The salt was taken up in methylene chloride/1N HCl and the aqueous layer was washed with two additional portions of methylene chloride. The organic layers were combined and evaporated and the residue was chromatographed by flash silica gel chromatography. Elution was carried out with a gradient ranging from 0 to 5% methanol/methylene chloride. The appropriate fractions were combined and evaporated to give 3-{4-[2-(ethylsulfonyl)ethyl]bicyclo[2.2.2]oct-1-yl}-4-methyl-5-[2-(trifluoromethyl)phenyl]-4H-1,2,4-triazole (10-6) as a white powder.
MS (ESI⁺)=456.2 (M+1); ¹H NMR (500 MHz, CDCl₃): δ 1.46 (3H, t, J=7.3 Hz), 1.63 (6H, m), 1.78 (2H, m), 2.19 (6H, m), 2.96 (2H, m), 3.05 (2H, q, J=7.2 Hz), 3.50 (3H, s), 7.56 (1H, m), 7.72 (2H, m), 7.87 (1H, m) ppm.

EXAMPLE 11

3-{4-[3-(Ethylsulfonyl)propyl]bicyclo[2.2.2]oct-1-yl}-4-methyl-5-[2-(trifluoromethyl)phenyl]-4H-1,2,4-triazole (11-10)

Step A:

(Benzyloxycarbonylmethyl)triphenylphosphonium bromide (4.6 g, 9.4 mmol) was azeotroped twice from toluene, and then suspended in 30 mL dry THF. Potassium hexamethyldisilazide (0.5 M in toluene, 16.8 mL, 8.4 mmol) was added dropwise at room temperature and the yellow solution was allowed to stir for 1 h, after which time it became milky white. A solution of 4-carbomethoxybicyclo[2.2.2]octane-1-carboxaldehyde (11-1) (0.50 g, 2.55 mmol) (Adcock, W., Kok, G. B. J. Org. Chem. 50: 1079-1087 (1985)) and benzoic acid (0.015 g, 0.13 mmol) in 2 mL of dry THF was prepared and added dropwise by syringe at room temperature. The mixture was heated to 90° C. and allowed to stir at reflux temperature, after which time the mixture was diluted with 200 mL of ethyl acetate and washed consecutively with 50 mL portions of 1 N HCl (twice), saturated aq. sodium bicarbonate, and brine. The organic layer was dried using magnesium sulfate, and the solvent was removed under reduced pressure. The residue was chromatographed on silica, eluting with a gradient of 5% to 10% ethyl acetate in hexane to provide methyl 4-[(1E)-3-(benzyloxy)-3-oxoprop-1-en-1-yl]bicyclo[2.2.2]octane-1-carboxylate (11-2) as a colorless oil. ¹H NMR (500 MHz, CDCl₃): δ 7.4 (5H, m), 6.94 (1H, d, J=17 Hz), 5.77 (1H, d, J=17 Hz), 5.21 (2H, s), 3.69 (3H, s), 1.86 (6H, m), 1.63 (6H, m) ppm.

Step B:

Diester 11-2 (0.625 g, 1.90 mmol) was dissolved in a 1:1 mixture of ethyl acetate/methanol (30 mL), placed under nitrogen atmosphere, then treated with 10% Pd/C (500 mg) and 0.1 mL of acetic acid. The reaction was placed under hydrogen atmosphere and stirred vigorously for 2 hr. The resulting solution was filtered through celite and the solvent was removed under reduced pressure. The residue was partitioned between 200 mL of ethyl acetate and 200 mL of 1 N NaOH solution. The aqueous layer was separated and neutralized, then extracted three times with 50 mL of methylene chloride. The combined organic layers were dried over magnesium sulfate and the solvent was removed under reduced pressure to afford 3-[4-(methoxycarbonyl)bicyclo[2.2.2]oct-1-yl]propanoic acid (11-3). ¹H NMR (500 MHz, CDCl₃): δ 3.62 (3H, s), 2.20 (2H, broad t, J=9 Hz), 1.75 (6H, m), 1.47 (2H, broad t, J=9 Hz), 1.38 (6H, m) ppm.

Step C:

Carboxylic acid 11-3 (400 mg, 1.67 mmol) was dissolved in tetrahydrofuran (5 mL) and borane (1 M solution in THF, 2.17 mL, 1.3 eq.) was added dropwise at room temperature. After 2 h the reaction was added to 50 mL of 1 N HCl and then extracted three times with 50 mL of methylene chloride. The combined organic layers were dried over magnesium sulfate and the solvent was removed under reduced pressure to afford crude methyl 4-(3-hydroxypropyl)bicyclo [2.2.2]octane-1-carboxylate (11-4) which was used without purification in the next step. ¹H NMR (500 MHz, CD₃OD): δ 3.66 (3H, s), 3.62 (2H, t, J=6.5 Hz), 1.78 (6H, m), 1.50 (2H, m), 1.41 (2H, m), 1.17 (2H, m) ppm.

Step D:

Hydroxyester 11-4 (430 mg, 1.9 mmol) was dissolved in 2.5 mL of anhydrous methylene chloride under nitrogen atmosphere, treated with pyridine (0.5 mL) and methanesulfonyl chloride (0.368 mL, 4.8 mmol) and stirred for 4 h at room temperature. The mixture was diluted with 100 mL of ethyl acetate and washed with 1N aqueous HC1, saturated aqueous sodium bicarbonate, and brine. The organic layer was dried over anhydrous sodium sulfate and evaporated. The crude methyl 4-{3-[(methylsulfonyl)oxy]propyl}bicyclo-[2.2.2]octane-1-carboxylate (11-5) thus afforded was used without purification in the next reaction. ¹H NMR (500 MHz, CDCl₃): δ 4.22 (2H, t, J=7.5 Hz), 3.68 (3H, s), 3.04 (3H, s), 1.82 (6H, m), 1.70 (2H, m), 1.44 (6H, m), 1.24 (2H, m) ppm.

Step E:

Mesylate 11-5 (3.30 g, 10.9 mmol) was dissolved in DMF (20 mL) and treated with sodium ethanethiolate (1.82 g, 21.7 mmol). The solution was stirred at 45° C. for 3 h, then the mixture was diluted with 100 mL of ethyl acetate and washed twice with 1N aqueous HC1, then with saturated aqueous sodium bicarbonate, and brine. The organic layer was dried over anhydrous sodium sulfate and evaporated to afford methyl 4-[3-(ethylthio)propyl]bicyclo[2.2.2]octane-1-carboxylate (11-6) as a crude oil which was used without purification in the next step.
¹H NMR (500 MHz, CDCl₃): δ 3.68 ppm (3H, s), 2.56 (2H, q, J=7 Hz), 2.51 (2H, t, J=7.5 Hz), 1.80 (6H, m), 1.52 (2H, m), 1.42 (6H, m), 1.28 (2H, t, J=7 Hz), 1.02 (2H, m).

Step F:

Sulfide 11-6 (3.0 g, 11 mmol) was dissolved in methylene chloride (50 mL) and treated with m-chloroperbenzoic acid (75%, 6.2 g). The solution was stirred at room temperature for 2 h, then the mixture was diluted with 100 mL of methylene chloride and washed with saturated aqueous sodium bicarbonate, then twice with saturated aqueous sodium bisulfite, then twice with saturated aqueous sodium bicarbonate, and brine. The organic layer was dried over anhydrous sodium sulfate and evaporated to afford methyl 4-[3-(ethylsulfonyl)propyl]bicyclo[2.2.2]octane-1-carboxylate (11-7) as a crude oil which was used without purification in the next step. ¹H NMR (500 MHz, CDCl₃): δ 3.68 ppm (3H, s), 2.56 (2H, q, J=7 Hz), 2.51 (2H, t, J=7.5 Hz), 1.80 (6H, m), 1.52 (2H, m), 1.42 (6H, m), 1.28 (2H, t, J=7 Hz), 1.02 (2H, m) ppm.

Step G:

Sulfone 11-7 (3.1 g, 10 mmol) was dissolved in 9:1 MeOH/water (50 mL) and treated with potassium hydroxide (3 g). The solution was stirred at room temperature overnight, then the mixture was acidified with 1 N HCl and extracted four times with 50 mL of methylene chloride. The organic layer was dried over anhydrous sodium sulfate and evaporated to afford 4-[3-(ethylsulfonyl)propyl]bicyclo[2.2.2]octane-1-carboxylic acid (11-8) which was used without purification in the next step. ¹H NMR (500 MHz, CDCl₃): δ 3.03 (2H, q, J=7 Hz), 2.94 (2H, dd, J=7.5 Hz), 1.84 (8H, m), 1.45 (8H, m), 1.30 (2H, m) ppm.

Step H:

Carboxylic acid 11-8 (3.0 g, 11 mmol) was dissolved in 50 mL of anhydrous methylene chloride under nitrogen atmosphere, treated with oxalyl chloride (2 M in methylene chloride, 16.2 mL, 32.4 mmol) and subsequently with 5 drops of DMF. The reaction was stirred at room temperature under nitrogen atmosphere for 90 min, then evaporated and placed under vacuum for 20 min. The acid chloride was dissolved in anhydrous methylene chloride (12 mL), cooled in an ice-bath, and then treated dropwise with a solution of methylamine (2M in THF, 27 mL, 54 mmol). Upon addition of the methylamine, the cooling bath was removed and the reaction stirred at ambient temperature for 30 min. The mixture was diluted with 200 mL of methylene chloride and washed with 1N aqueous HC1, saturated aqueous sodium bicarbonate, and brine. The organic layer was dried over anhydrous sodium sulfate and evaporated. The residue was subjected to chromatography on silica gel eluting with a gradient from 0 to 3% methanol in ethyl acetate to give 4-[3-(ethylsulfonyl)propyl]-N-methylbicyclo[2.2.2]octane-1-carboxamide 11-9 as a white powder. MS (ESI⁺)=302 (M+1).
¹H NMR (500 MHz, CDCl₃): δ 5.56 (1H, br s), 3.02 (2H, q, J=7 Hz), 2.94 (2H, dd, J=7.5 Hz), 2.82 (3H, d, J=4 Hz), 1.80 (8H, m), 1.45 (9H, m), 1,28 (2H, m) ppm.

Step I:

Methyl amide 11-9 (0.470 g, 1.56 mmol) was dissolved in anhydrous methylene chloride (5 mL) and treated with oxalyl chloride (2M in methylene chloride, 1.56 mL, 3.12 mmol) and DMF (2 drops). The solution was stirred at room temperature for 1 h, then solvent removed by evaporation under reduced pressure. The residue was redissolved in anhydrous toluene (7 mL) and treated with 5 [2-(trifluoromethyl)phenyl]1H-tetrazole (368 mg, 1.72 mmol). The mixture was refluxed for 18 h. The reaction was cooled to room temperature and the precipitate was filtered and washed to give 300 mg of crude product as the HCl salt. The salt was taken up in methylene chloride/1N HCl and the aqueous layer was washed with two additional portions of methylene chloride. The organic layers were combined and evaporated and the residue was chromatographed by flash silica gel chromatography. Elution was carried out with a gradient ranging from 0 to 5% methanol/methylene chloride. The appropriate fractions were combined and evaporated to give 3-{4-[3-(ethylsulfonyl)propyl]bicyclo[2.2.2]oct-1-yl}-4-methyl-5-[2-(trifluoromethyl)phenyl]-4H-1,2,4-triazole (11-10) as a white powder.
MS (ESI)=470.4 (M+1). ¹H NMR (500 MHz, CDCl₃): δ 7.87 (1H, m), 7.72 (2H, m), 7.56 (1H, m), 3.49 (3H, s), 3.05 (2H, q, J=7.2 Hz), 2.96 (2H, m), 2.18 (6H, m), 1.86 (2H, m), 1.62 (6H, m), 1.46 (3H, t, J=7.3 Hz), 1.36 (2H, m) ppm.

EXAMPLE 12

4-Methyl-3-{-4-[4-(methylsulfonyl)phenyl]bicyclo[2.2.2]oct-1-yl}-5-[2-(trifluoromethyl)phenyl]-4H-1,2,4-triazole (12-G)

Step A:

To a stirred solution of methyl 4-phenylbicyclo[2.2.2]octane-1-carboxylate 12-A (Chapman, N. B. et al. J. Org. Chem., 1970, 35, 917) (4.80 g, 19.6 mmol) in 1,2-dichloroethane (2 ml, 1 M) was added methanesulfonyl fluoride (4.05 ml, 58.9 mmol) followed by aluminum trichloride (9.17 g, 68.8 mmol). The reaction mixture was stirred overnight under nitrogen atmosphere at ambient temperature followed by addition of another portion of methanesulfonyl fluoride (4.05 ml, 58.9 mmol) and aluminum trichloride (9.17 g, 68.8 mmol). The resulting mixture was heated at 80° C. for 3 h, then cooled to room temperature and diluted with 300 ml of dichloromethane and 200 ml water. The layers were separated and the aqueous layer was washed with two 100 ml portions of dichloromethane. The organic layers were combined, washed with brine, dried (MgSO₄), and concentrated in vacuo. The crude product was chromatographed on normal phase flash silica gel column, eluting with a gradient 10-50% EtOAc/hexanes to yield 1.4 g of 12-B (>95% pure). The material was recrystallized from EtOAc to yield compound 12-B.
¹H NMR (500 MHz, CDCl₃): δ 1.93 (6H, m), 1.99 (6H, m), 3.08 (3H, s), 3.73 (3H, s), 7.55 (2H, d, J=8.3 Hz), 7.90 (2H, d, J=8.1 Hz) ppm.

Step B:

Carboxylic acid 12-C was prepared in quantitative yield by hydrolysis of ester 12-B (1.1 g, 3.4 mmol) using the procedures described in Example 11, Step G. ¹H NMR (500 MHz, CDCl₃): δ 1.98 (6H, m), 2.04 (6H, m), 3.11 (3H, s), 7.58 (2H, d, J=7.8 Hz), 7.92 (2H, d, J=7.9 Hz) ppm.

Step C:

Carboxylic acid 12-C (0.99 g, 3.2 mmol) was converted to hydrazide 12-D using hydrazine (0.124 ml, 4 mmol) and the standard coupling procedure analogous to Example 9, step A. Crude product was purified by flash silica gel chromatography eluting with 0-2% MeOH/CH₂Cl₂gradient to yield a white powder. MS (ESI⁺)=323.2 (M+1).

Step D:

To a suspension of 12-D (0.67 g, 2.1 mmol) in EtOH (11 ml) was added aldehyde 12-E (0.36 g, 2.1 mmol) and the mixture was refluxed for 18 h. The solvent was removed in vacuo and the solid residue was heated in thionyl chloride (2.9 ml, 40 mmol) for 2 h at 75° C. then stripped to dryness. This residue was treated with methylamine (2M THF, 2 ml) and methylamine (40% aqueous, 1 ml) for 18 h at 70° C. The volatiles were removed in vacuo and the solid was chromatographed on a flash silica gel column using a 10-25% acetone/hexanes gradient to yield compound 12-F. MS (ESI⁺)=492.3 (M+1); ¹H NMR (500 MHz, CDCl₃) (2 isomers ratio 3:2): major isomer: δ 2.00 (6H, m), 2.14 (6H, m), 3.10 (3H, s), 3.28 (3H, d, J=5.1 Hz), 5.71 (1H, br. s), 7.47 (1H, m), 7.59 (3H, m), 7.72 (1H, d, J=7.9 Hz), 7.92 (2H, m), 8.26 (1H, d, J=7.9 Hz), 8.70 (1H, br. s) ppm; minor isomer: δ 2.00 (6H, m), 2.32 (6H, m), 2.98 (3H, d, J=4.7 Hz), 3.10 (3H, s), 4.70 (1H, br. s), 7.47 (1H, m), 7.59 (4H, m), 7.92 (2H, m), 8.30 (1H, d, J=7.8 Hz), 8.56 (1H, br. s) ppm.

Step E:

A solution of 12-F (0.58 g, 1.2 mmol) in EtOH (5 ml) was heated to 40° C. then treated with a solution of ferric chloride (0.4 g, 2.4 mmol) in water (1 ml). The resulting mixture was heated at 90° C. for 18 h. Another portion of ferric chloride (0.4 g, 2.4 mmol) was added and the reaction heated at 90° C. for 24 h. The volatiles were removed in vacuo and the solid was redissolved in CH₂Cl₂and washed with a saturated aqueous solution of EDTA and brine then dried (MgSO₄) and stripped. The crude product was purified and isolated using the conditions described for purification of 4-J (Example 4, step G) to yield compound 12-G.
MS (ESI⁺)=490.3 (M+1); ¹H NMR (500 MHz, CDCl₃): δ 2.06 (6H, m), 2.31 (6H, m), 3.08 (3H, s), 3.52 (3H, s), 7.52 (1H, m), 7.59 (2H, d, J=8.4 Hz), 7.71 (2H, m), 7.86 (1H, m), 7.92 (2H, d, J=8.6 Hz) ppm.

EXAMPLE 13

3-(4-{4-Methyl-5-[2-(trifluoromethyl)phenyl]-4H-1,2,4-triazol-3-yl}bicyclo [2.2.2]oct-1-yl)-5-(3,3,3-trifluoropropyl)-1,2,4-oxadiazole (13-F)

Step A:

4-(Methoxycarbonyl)bicyclo[2.2.2]octane-1-carboxylic acid 13-A (Chapman, N. B. et al. J. Org. Chem., 1970, 35, 917) (4.0 g, 18.9 mmol) was converted to methyl 4-[(methylamino)carbonyl]bicyclo[2.2.2]octane-1-carboxylate 13-B using the methods described in Example 10, steps C and D. Product was purified by flash silica gel chromatography, eluting with 0-5% MeOH/CH₂Cl₂gradient to yield a white solid. MS (ESI⁺)=226.2 (M+1).

Step B:

Methyl 4-[(methylamino)carbonyl]bicyclo[2.2.2]octane-1-carboxylate 13-B (2.76 g, 12.3 mmol) was converted to 1, 2, 4-triazole 13-C using the procedures described in Example 10, Step E. The product, which precipitated out of reaction mixture as the HCl salt, was dissolved in CH₂Cl₂, washed twice with saturated aqueous sodium bicarbonate solution, dried (MgSO₄) and stripped to yield a white solid. MS (ESI⁺)=394.2 (M+1);
¹H NMR (500 MHz, CDCl₃): δ 2.00 (6H, m), 2.18 (6H, m), 3.48 (3H, s), 3.72 (3H, s), 7.51 (1H, m), 7.71 (2H, m), 7.85 (1H, m) ppm.

Step C:

A solution of methyl ester 13-C (1.19 g, 3.0 mmol) in 5% H₂O/MeOH (30 ml) was treated with KOH (0.51 g, 9.0 mmol) at 60° C. under nitrogen atmosphere for 18 h. The resulting mixture was concentrated down, diluted with water (150 ml), washed with EtOAc and acidified with aqueous HCl (1 N) to pH=3. The precipitate was filtered, washed with a small amount of water and ether and dried under vacuum to yield a pink solid (0.87 g, 76%). A portion of the solid (0.67 g, 1.77 mmol) was suspended in CH₂Cl₂(15 ml) and treated with carbonyldiimidazole (0.57 g, 3.54 mmol) at room temperature and nitrogen atmosphere. After 2 h, concentrated ammonium hydroxide was added (40 ml) and the reaction was stirred for 18 h. The crude mixture was diluted with water (150 ml) and extracted with 3 portions of CH₂Cl₂(70 ml). The organic washes were combined, washed with brine, dried (Na₂SO₄), and stripped to yield compound 13-D as a white powder. MS (ESI⁺)=379.3 (M+1).

Step D:

A solution of carboxamide 13-D (0.64 g, 1.7 mmol) and cyanuric chloride (0.47 g, 2.53 mmol) in DMF (15 ml) was stirred at room temperature under nitrogen atmosphere. After 2 h, DMF was removed in vacuo and the solid was redissolved in CH₂Cl₂(100 ml) and washed with saturated aqueous sodium bicarbonate and brine, dried (Na₂SO₄), and stripped to give the nitrile 13-E as a pale yellow solid. MS (ESI⁺)=361.3 (M+1); ¹H NMR (500 MHz, CDCl₃): δ 2.15 (6H, m), 2.22 (6H, m), 3.47 (3H, s), 7.51 (1H, m), 7.72 (2H, m), 7.87 (1H, m) ppm.

Step E:

A solution of nitrile 13-E (0.56 g, 1.6 mmol) and hydroxylamine (50% aqueous, 4 ml) in ethanol (40 ml) was heated at 80° C. for 18 h. The resulting mixture was cooled to room temperature and concentrated in vacuo. The solid was suspended in toluene, the solvent removed in vacuo, and the solid was dried under reduced pressure. A portion of the resulting white powder (0.050 g, 0.13 mmol) was added to a pre-stirred solution of 4,4,4-trifluorobutyric acid (0.072 g, 0.51 mmol) and carbonyldiimidazole (0.082 g, 0.51 mmol) in CH₂Cl₂(3 ml). The resulting mixture was stirred at room temperature for 48 h, then concentrated down. The solid was resuspended in toluene and refluxed under nitrogen atmosphere for 3 h. The crude product was purified and isolated using the conditions described for purification of 4-J (Example 4, step G) to yield 13-F as a white powder.
MS (ESI⁺)=500.2 (M+1); ¹H NMR (500 MHz, CDCl₃): δ 2.12 (6H, m), 2.30 (6H, m), 2.73 (2H, m), 3.18 (2H, m), 3.54 (3H, s), 7.61 (1H, m), 7.74 (2H, m), 7.87 (1H, m) ppm.

EXAMPLE 14

3-(4-{-4-Methyl-5-[2-(trifluoromethyl)phenyl]-4H-1,2,4-triazol-3-yl}bicyclo [2.2.2]oct-1-yl)-5-(3,3,3-trifluoroethyl)-1,2,4-oxadiazole (14-B)

Step A:

Triazole 14-B was prepared from nitrile 13-E (0.053 g, 0.14 mmol) and 3,3,3-trifluoromethylpropionic acid (0.036 ml, 0.41 mmol) using the method described in Example 13, step E. 3-(4-{4-Methyl-5-[2-(trifluoromethyl)phenyl]-4H-1,2,4-triazol-3-yl}bicyclo [2.2.2]oct-1-yl)-5-(3,3,3-trifluoroethyl)-1,2,4-oxadiazole (14-B) was isolated as a white powder. MS (ESI⁺)=486.2 (M+1); ¹H NMR (500 MHz, CDCl₃): δ 2.14 (6H, m), 2.31 (6H, m), 3.53 (3H, s), 3.81 (2H, q, J=9.5 Hz), 7.57 (1H, m), 7.73 (2H, m), 7.87 (1H, m) ppm.

EXAMPLE 15

4-Methyl-3-[2-(trifluoromethyl)phenyl]-5-(4-{2-[(trifluoromethyl)sulfonyl]ethyl}bicyclo[2.2.2]oct-1-yl)-4H-1,2,4-triazole (15-G)

Step A:

To a stirred solution of methyltriphenylphosphonium bromide (9.1 g, 12.8 mmol) in THF (50 ml) at 0° C. was added potassium hexamethyldisilazide (0.5M in toluene, 48.6 ml), dropwise over 5 min. The resulting mixture was allowed to warm up to room temperature over 1 h, then cooled again to 0° C. and treated with methyl 4-formylbicyclo[2.2.2]octane-1-carboxylate 15-A (Chapman, N. B. et al. J. Org. Chem., 1970, 35, 917) (2.5 g, 12.8 mmol). The reaction mixture was stirred at room temperature for 18 h then diluted with EtOAc (350 ml). The organic phase was washed with aqueous HCl (1 N), saturated aqueous sodium bicarbonate, and brine, then dried (Na₂SO₄) and concentrated in vacuo. The resulting solid was purified by flash silica gel chromatography, eluting with a gradient 0-4% EtOAc/hexanes. The resulting methyl 4-vinylbicyclo[2.2.2]octane-1-carboxylate 15-B was isolated as a clear, colorless oil.

Step B:

To a stirred solution of olefin 15-B (1.6 g, 8.3 mmol) in THF (20 ml) was added 9-BBN (0.5M in THF, 49 ml), dropwise. The solution was allowed to stir at room temperature for 18 h, then treated sequentially with ethanol (14.5 ml), aqueous NaOH (5N, 5 ml), and hydrogen peroxide (30% aqueous, 9.7 ml). The reaction mixture was acidified to pH=2 with aqueous HCl (1 N) and extracted three times with CH₂Cl₂. The organic layers were combined, washed with brine, dried (Na₂SO₄), and stripped. The resulting alcohol 15-C was purified by silica gel chromatography eluting with a gradient 30-50% EtOAc/hexanes and isolated as a clear, colorless oil.

Step C:

A solution of alcohol 15-C (1.5 g, 7.1 mmol) in CH₂Cl₂(7.5 ml), pyridine (1.5 ml) was cooled to 0° C. and treated with methanesulfonyl chloride (1.65 ml, 21.3 mmol), dropwise over 5 min. The reaction mixture was allowed to warm to room temperature, then stirred for 3 h. EtOAc (300 ml) was added and the organic phase was washed with aqueous HCl (1 N) three times, saturated aqueous sodium bicarbonate two times, and brine. The organic layer was dried (Na₂SO₄), and stripped to yield methyl 4-{2-[(methylsulfonyl)oxy]ethyl}bicyclo[2.2.2]octane-1-carboxylate 15-D as a white solid. ¹H NMR (500 MHz, CDCl₃): δ 1.52 (6H, m), 1.66 (2H, t, J=7.1 Hz), 1.84 (6H, m), 3.04 (3H, s), 3.69 (3H, s), 4.29 (2H, t, J=7.2 Hz) ppm.

Step D:

A solution of 15-D (0.25 g, 0.86 mmol), potassium trifluoromethanesulfinate (0.3 g, 1.72 mmol), and tetrabutylammonium iodide (0.15 g, 0.4 mmol) in DMF (5 ml) was heated at 140° C. for 5 h. under nitrogen atmosphere. The solution was then cooled to room temperature and diluted with EtOAc (100 ml) and washed with aqueous HCl (IN) two times and brine. The organic layer was dried (Na₂SO₄), stripped, and chromatographed on flash silica gel, eluting with a gradient 5-20% EtOAc/hexanes. The resulting trifluoromethylsulfone 15-E was isolated as a white solid. ¹H NMR (500 MHz, CDCl₃): δ 1.50 (6H, m), 1.78 (2H, m), 1.82 (6H, m), 3.17 (2H, m), 3.67 (3H, s) ppm.

Step E:

Methyl ester 15-E (0.035 g, 0.11 mmol) was converted to the methyl amide 15-F using the methods described in Example 10, steps C and D. The N-methyl-4-{2-[(trifluoromethyl)sulfonyl]ethyl}bicyclo[2.2.2]octane-1-carboxamide was isolated as a white solid; MS (ESI⁺)=328.2 (M+1).

Step F:

Methyl amide 15-F (0.030 g, 0.092 mmol) was converted to triazole 15-G using the procedures outlined in Example 10, step E. 4-Methyl-3-[2-(trifluoromethyl)phenyl]-5-(4-{2-[(trifluoromethyl)sulfonyl]ethyl}bicyclo[2.2.2]oct-1-yl)-4H-1,2,4-triazole (15-G) was isolated as a white powder; MS (ESI⁺)=496.4 (M+1).

EXAMPLES 16-150

Following procedures similar to those described above, the following compounds of formula II were also prepared:


	(II)

				Parent
				Ion
Ex. #	R³	R²	R¹	m/z

16		CH₃	338

17		CH₃	406

18		CH₃	352

19		CH₃	372

20		CH₃	356

21		CH₃	368

22		CH₃	384

23		CH₃	383

24		CH₃	416

25		CH₃	422

26		CH₃	354

27		CH₃	382

28		CH₃	422

29		CH₃	368

30		CH₃	354

31		CH₃	496

32		CH₃	417

33		CH₃	372

34		CH₃	372

35		CH₃	352

36		CH₃	398

37		CH₃	382

38		CH₃	400

39		CH₃	368

40		CH₃	356

41		CH₃	366

42		CH₃	398

43		CH₃	374

44		CH₃	404

45		CH₃	374

46		CH₃	372

47		CH₃	372

48		CH₃	378

49		CH₃	402

50		CH₃	436

51		CH₃	370

52		CH₃	422

53		CH₃	366

54		CH₃	431

55		CH₃	382

56		CH₃	430

57		CH₃	414

58		CH₃	418

59		CH₃	474

60		CH₃	430

61		CH₃	353

62		CH₃	363

63		CH₃	366

64		CH₃	339

65		CH₃	339

66		CH₃	339

67		CH₃	355

68		CH₃	340

69		CH₃	388

70		CH₃	388

71		CH₃	378

72		CH₃	377

73		CH₃	389

74		CH₃	377

75		CH₃	380

76		CH₃	380

77		CH₃	382

78		CH₃	378

79		CH₂CH₃	382

80		CH₂CH₃	382

81		CH₂CH₃	352

82		CH₂CH₃	368

83		CH₂CH₃	380

84		CH₂CH₃	366

85			364

86		CH₃	373

87		CH₃	389

88			365

89		CH₃	340

90		CH₃	378

91		CH₃	345

92		CH₃	340

93		CH₃	326

94		CH₃	354

95		CH₃	361

96		CH₃	356

97		CH₃	340

98		CH₃	343

99		CH₃	338

100		CH₃	364

101		CH₃	326

102		CH₃	312

103		CH₃	331

104		CH₃	342

105		CH₃	326

106		CH₃	340

107		CH₃	361

108		CH₃	356

109		CH₃	365

110		CH₃	382

111		CH₃	358

112		CH₃	379

113		CH₃	407

114		CH₃	438

115	CH₃	CH₃	282

116		CH₃	348

117	H	CH₃	284

118	H	CH₃	298

119	H	CH₃	302

120	H	CH₃	298

121	H	CH₃	336

122		CH₃	344

123		CH₃	374

124		CH₃	358

125		CH₃	360

126		CH₃	471

127		CH₃	456

128		CH₃	326

129	CbzNH—	CH₃	428

130	NH₂	CH₃	313

131		CH₃	450

132		CH₃	421

133		CH₃	422

134		CH₃	402

135		CH₃	456

136		CH₃	470

137		CH₃	442

138		CH₃	440

139		CH₃	470

140		CH₃	484

141		CH₃	490

142		CH₃	508

143		CH₃	420

Furthermore following procedure similar to those described above, the following compounds of formula III were also prepared:


	(III)

			Parent
			Ion
Ex. #	R⁵	R¹	m/z

144	Cl		477

145	Cl		515

146	Cl		480

147	Cl		511

148	Cl		497

149	F		476

150	F		567

Example of a Pharmaceutical Formulation

As a specific embodiment of an oral composition of a compound of the present invention, 50 mg of any of Examples 1-15 is formulated with sufficient finely divided lactose to provide a total amount of 580 to 590 mg to fill a size O hard gelatin capsule.
While the invention has been described and illustrated in reference to specific embodiments thereof, those skilled in the art will appreciate that various changes, modifications, and substitutions can be made therein without departing from the spirit and scope of the invention. For example, effective dosages other than the preferred doses as set forth hereinabove may be applicable as a consequence of variations in the responsiveness of the human being treated for a particular condition. Likewise, the pharmacologic response observed may vary according to and depending upon the particular active compound selected or whether there are present pharmaceutical carriers, as well as the type of formulation and mode of administration employed, and such expected variations or differences in the results are contemplated in accordance with the objects and practices of the present invention. It is intended therefore that the invention be limited only by the scope of the claims which follow and that such claims be interpreted as broadly as is reasonable.

Claims

1. A compound which is

or a pharmaceutically acceptable salt thereof.

2. A method of treating cognitive impairment in a mammalian patient comprising administering to the patient an effective amount of a compound selected from the group consisting of structural formula I:

or a pharmaceutically acceptable salt thereof; wherein

each p is independently 0, 1, or 2;

each n is independently 0, 1, or 2;

X is selected from the group consisting of a single bond, O, S(O)_p, NR⁶,

R¹is selected from the group consisting of

arylcarbonyl,

(CH₂)_n-aryl, and

(CH₂)_n-heteroaryl;

in which aryl and heteroaryl are unsubstituted or substituted with one to three substituents independently selected from R⁵;

R²is selected from the group consisting of

hydrogen,

C_1-8alkyl,

C_2-6alkenyl, and

(CH₂)_n—C_3-6cycloalkyl;

in which alkyl, alkenyl, and cycloalkyl are unsubstituted or substituted with one to three substituents independently selected from R⁸and oxo;

each R⁴is independently selected from the group consisting of

hydrogen,

halogen,

hydroxy,

oxo,

C_1-3alkyl, and

C_1-3alkoxy;

R³is selected from the group consisting of

hydrogen,

C_1-10alkyl

C_2-10alkenyl,

(CH₂)_n—C_3-6cycloalkyl,

(CH₂)_n-aryl, and

(CH₂)_n-heteroaryl;

(CH₂)_n-heterocyclyl;

in which aryl, heteroaryl and heterocyclyl are unsubstituted or substituted with one to three substituents independently selected from R⁵; and alkyl, alkenyl, and cycloalkyl are unsubstituted or substituted with one to five groups independently selected from R⁸and oxo;

R⁵and R⁸are independently selected from the group consisting of

hydrogen,

formyl,

C_1-6alkyl,

(CH₂)_n-aryl,

(CH₂)_n-heteroaryl,

(CH₂)_n-heterocyclyl,

(CH₂)_nC_3-7cycloalkyl,

halogen,

OR⁷,

(CH₂)_nN(R⁷)₂,

cyano,

(CH₂)_nCO₂R⁷,

NO₂,

(CH₂)_nNR⁷SO₂R⁶,

(CH₂)_nSO₂N(R⁷)₂,

(CH₂)_nS(O)_pR⁶,

(CH₂)_nSO₂₀R⁷,

(CH₂)_nNR⁷C(O)N(R⁷)₂,

(CH₂)_nC(O)N(R⁷)₂,

(CH₂)_nNR⁶C(O)R⁶,

(CH₂)_nNR⁶CO₂R⁷,

O(CH₂)_nC(O)N(R⁷)₂,

CF₃,

CH₂CF₃,

OCF₃,

OCHCF₂, and

OCH₂CF₃;

wherein aryl, heteroaryl, cycloalkyl, and heterocyclyl are unsubstituted or substituted with one to three substituents independently selected from halogen, hydroxy, C_1-4alkyl, trifluoromethyl, trifluoromethoxy, and C_1-4alkoxy; and wherein any methylene (CH₂) carbon atom in R⁵and R⁸is unsubstituted or substituted with one to two groups independently selected from halogen, hydroxy, and C_1-4alkyl; or two substituents when on the same methylene (CH₂) carbon atom are taken together with the carbon atom to which they are attached to form a cyclopropyl group;

each R⁶is independently selected from the group consisting of

C_1-8alkyl,

(CH₂)_n-aryl,

(CH₂)_n-heteroaryl, and

(CH₂)_nC_3-7cycloalkyl;

wherein alkyl and cycloalkyl are unsubstituted or substituted with one to five substituents independently selected from halogen, oxo, C_1-4alkoxy, C_1-4alkylthio, hydroxy, amino; and aryl and heteroaryl are unsubstituted or substituted with one to three substituents independently selected from cyano, halogen, hydroxy, amino, carboxy, trifluoromethyl, trifluoromethoxy, C_1-4alkyl, and C_1-4alkoxy;

or two R⁶groups together with the atom to which they are attached form a 5- to 8-membered mono- or bicyclic ring system optionally containing an additional heteroatom selected from O, S, and NC_1-4alkyl;

and

each R⁷is hydrogen or R⁶;

structural formula II, or a pharmaceutically acceptable salt thereof

(II)

wherein R³ R² R¹

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₂CH₃

CH₃

CH₃ CH₃

CH₃

H CH₃

CH₃

CbzNH— CH₃

NH₂ CH₃

CH₃

CH₃

CH₃

and structural formula III, or a pharmaceutically acceptable salt thereof

(III)

wherein R^5′ R¹ Cl

Cl

F

or a pharmaceutically acceptable salt thereof.

3. A method in accordance with claim 2, wherein the compound administered is selected from the group consisting of:

or a pharmaceutically acceptable salt thereof.

4. A method in accordance with claim 2, wherein the compound administered is selected from the group consisting of

or a pharmaceutically acceptable salt thereof.

5. A method of delaying the onset of cognitive impairment in a mammalian patient in need thereof comprising administering to the patient an effective amount of a compound selected from the group consisting of structural formula I:

or a pharmaceutically acceptable salt thereof; wherein

each p is independently 0, 1, or 2;

each n is independently 0, 1, or 2;

X is selected from the group consisting of a single bond, O, S(O)_p, NR⁶,

R¹is selected from the group consisting of

arylcarbonyl,

(CH₂)_n-aryl, and

(CH₂)_n-heteroaryl;

R²is selected from the group consisting of

hydrogen,

C_1-8alkyl,

C_2-6alkenyl, and

(CH₂)_n—C_3-6cycloalkyl;

each R⁴is independently selected from the group consisting of

hydrogen,

halogen,

hydroxy,

oxo,

C_1-3alkyl, and

C_1-3alkoxy;

R³is selected from the group consisting of

hydrogen,

C_1-10alkyl

C_2-10alkenyl,

(CH₂)_n—C_3-6cycloalkyl,

(CH₂)_n-aryl, and

(CH₂)_n-heteroaryl;

(CH₂)_n-heterocyclyl;

R⁵and R⁸are independently selected from the group consisting of

hydrogen,

formyl,

C_1-6alkyl,

(CH₂)_n-aryl,

(CH₂)_n-heteroaryl,

(CH₂)_n-heterocyclyl,

(CH₂)_nC_3-7cycloalkyl,

halogen,

OR⁷,

(CH₂)_nN(R⁷)₂,

cyano,

(CH₂)_nCO₂R⁷,

NO₂,

(CH₂)_nNR⁷SO₂R⁶,

(CH₂)_nSO₂N(R⁷)₂,

(CH₂)_nS(O)_pR⁶,

(CH₂)_nSO₂₀R⁷,

(CH₂)_nNR⁷C(O)N(R⁷)₂,

(CH₂)_nC(O)N(R⁷)₂,

(CH₂)_nNR⁶C(O)R⁶,

(CH₂)_nNR⁶CO₂R⁷, O(CH₂)_nC(O)N(R⁷)₂,

CF₃,

CH₂CF₃,

OCF₃,

OCHCF₂, and

OCH₂CF₃;

each R⁶is independently selected from the group consisting of

C_1-8alkyl,

(CH₂)_n-aryl,

(CH₂)_n-heteroaryl, and

(CH₂)_nC_3-7cycloalkyl;

and

each R⁷is hydrogen or R⁶;

structural formula II, or a pharmaceutically acceptable salt thereof

(II)

wherein R³ R² R¹

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₃

CH₂CH₃

CH₃

CH₃ CH₃

CH₃

H CH₃

CH₃

CbzNH— CH₃

NH₂ CH₃

CH₃

CH₃

CH₃

and structural formula III, or a pharmaceutically acceptable salt thereof

(III)

R^5′ R¹ Cl

Cl

F

or a pharmaceutically acceptable salt thereof.

6. A method in accordance with claim 5, wherein the compound administered is selected from the group consisting of:

or a pharmaceutically acceptable salt thereof.

7. A method in accordance with claim 5, wherein the compound administered is selected from the group consisting of

or a pharmaceutically acceptable salt thereof.

8. A method of reducing the risk of developing cognitive impairment in a mammalian patient in need thereof comprising administering to the patient an effective amount of a compound selected from the group consisting of structural formula I:

or a pharmaceutically acceptable salt thereof; wherein

each p is independently 0, 1, or 2;

each n is independently 0, 1, or 2;

X is selected from the group consisting of a single bond, O, S(O)_p, NR⁶,

R¹is selected from the group consisting of

arylcarbonyl,

(CH₂)_n-aryl, and

(CH₂)_n-heteroaryl;

R²is selected from the group consisting of

hydrogen,

C_1-8alkyl,

C_2-6alkenyl, and

(CH₂)_n—C_3-6cycloalkyl;

each R⁴is independently selected from the group consisting of hydrogen,

halogen,

hydroxy,

oxo,

C_1-3alkyl, and

C_1-3alkoxy;

R³is selected from the group consisting of

hydrogen,

C_1-10alkyl

C_2-10alkenyl,

(CH₂)_n—C_3-6cycloalkyl,

(CH₂)_n-aryl, and

(CH₂)_n-heteroaryl;

(CH₂)_n-heterocyclyl;

R⁵and R⁸are independently selected from the group consisting of

hydrogen,

formyl,

C_1-6alkyl,

(CH₂)_n-aryl,

(CH₂)_n-heteroaryl,

(CH₂)_n-heterocyclyl,

(CH₂)_nC_3-7cycloalkyl,

halogen,

OR⁷,

(CH₂)_nN(R⁷)₂,

cyano,

(CH₂)_nCO₂R⁷,

NO₂,

(CH₂)_nNR⁷SO₂R⁶,

(CH₂)_nSO₂N(R⁷)₂,

(CH₂)_nS(O)_pR⁶,

(CH₂)_nSO₂₀R⁷,

(CH₂)_nNR⁷C(O)N(R⁷)₂,

(CH₂)_nC(O)N(R⁷)₂,

(CH₂)_nNR⁶C(O)R⁶,

(CH₂)_nNR⁶CO₂R⁷,

O(CH₂)_nC(O)N(R⁷)₂,

CF₃,

CH₂CF₃,

OCF₃,

OCHCF₂, and

OCH₂CF₃;

each R⁶is independently selected from the group consisting of

C_1-8alkyl,

(CH₂)_n-aryl,

(CH₂)_n-heteroaryl, and

(CH₂)_nC_3-7cycloalkyl;

or two R⁶groups together with the atom to which they are attached form a 5- to 8-membered mono- or bicyclic ring system optionally containing an additional heteroatom selected from O, S, and NC_1-4alkyl; and

each R⁷is hydrogen or R⁶;

structural formula II, or a pharmaceutically acceptable salt thereof