[go: up one dir, main page]

US20080210700A1 - Thermocycler - Google Patents

Thermocycler Download PDF

Info

Publication number
US20080210700A1
US20080210700A1 US11/916,616 US91661606A US2008210700A1 US 20080210700 A1 US20080210700 A1 US 20080210700A1 US 91661606 A US91661606 A US 91661606A US 2008210700 A1 US2008210700 A1 US 2008210700A1
Authority
US
United States
Prior art keywords
thermocycler
base
wall
receiving segment
hermetic wall
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US11/916,616
Other languages
English (en)
Inventor
Henner Tasch
Bernd Kruger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Assigned to EPPENDORF AG reassignment EPPENDORF AG ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: TASCH, HENNER, KRUGER, BERND
Publication of US20080210700A1 publication Critical patent/US20080210700A1/en
Abandoned legal-status Critical Current

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/141—Preventing contamination, tampering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/142—Preventing evaporation

Definitions

  • thermocycler usually denotes apparatus allowing PCR (reactions).
  • reactions PCR is a well-established procedure known to the expert, this abbreviation being well understood to denote using polymerase on a test sample to amplify the target nucleic acids contained therein.
  • thermocyclers allowing measuring in real time the increase over time of the amplified products.
  • thermocyclers comprise a base in which is constituted an appropriate, temperature controlled receiving segment receiving reaction vials subjected to PCR.
  • an apparatus comprises a top displaceable from an open position into an operational position above the receiving segment. This top is fitted with a test optics which in the operational configuration is situated opposite the receiving segment and can detect optical signals in the reaction vials.
  • thermocycler allowing PCR to take place while free from extraneous factors, in particular in the real time mode, in simpler and more reliable manner.
  • thermocycler of the present invention is fitted with a hermetic wall running in a closed loop around the receiving segment and extending between the top and the base thereby sealing the top and the base.
  • the wall of the present invention effectively allows screening the PCR receiving segment against extraneous air effects for instance.
  • the wall of the present invention also may be fitted into conventional thermocyclers. Preferably however it shall be employed for real-time thermocyclers.
  • the hermetic wall is also light-tight and in the top's operational configuration surrounds both the receiving segment in the base and the test optics in the top, thereby providing a light path and a reaction space protected against external interference.
  • the wall might be kept in storage as a separate component and would be mounted on the base prior to PCR and illustratively would engage in sealing manner by its upper open end the top when this top has been lowered into its operational position.
  • the wall is used as a component joined to the thermocycler.
  • the wall as a rigid component might be projecting from the base or the top. In that case however there would be danger of degrading handling.
  • the wall shall project by the length required from the top respectively the base upward and downward only during PCR. In cases not requiring the wall, it might be retracted in its entirety into the top or the base.
  • the wall length is adjustable.
  • this feature illustratively can be attained in that the wall is in the form of a bellows affixed by one of its open ends for instance in a closed loop to the top and is able to be lowered at the other free open end by an appropriate adjustment device down on the base when the top is in its operational position.
  • one or more closed-loop silicone seals or the like may be used at the free wall end or at the preferably employed bellows. It is understood that such seals also may be used in other cases at the two open wall ends when a separate component is involved.
  • the bellows may be driven by a separate element at the top, for instance an adjusting wheel or the like. If drive elements already are present at the top to implement top adjustment or locking the top in position, the adjustable wall also may be adjusted in that manner. In that case the drive elements may be synchronized in a manner that when the top is displaced and locked into its operational position, the drive element used for such actuations also may be used to displace the wall of the present invention between the top and the base.
  • the wall may be configured in or on the base. Provided the wall is adjustable in length, it need not mandatorily be a bellows. Other applicable designs include longitudinally adjustable ones such as lamellas and telescopes.
  • FIG. 1 shows a schematic cross-section view of the present invention.
  • FIG. 1 schematically shows a cross-section of a thermocycler 10 comprising a base 11 fitted with a receiving segment 12 receiving reaction vials.
  • Heater elements 13 and 14 in particular Peltier elements, are used to control the temperature of the receiving segment 12 .
  • the diagrammatic recesses 15 receive the reaction vials to be temperature controlled.
  • thermocycler receiving segments are designed to receive microtitration trays (not shown).
  • thermocycler 10 comprises a top 18 , shown in its operational position, which in said position rests on protrusions 19 configured on the base 11 .
  • a downward looking test optics 20 is configured at the top 18 and is fitted with several test apertures 21 associated with the recesses 15 .
  • a particular amplification taking place in a reaction vial can always be optically measured by said test optics 20 .
  • a wall 22 in the form of a bellows is configured between the top 18 and the base 11 and surrounds the test optics 20 and the receiving segment 12 .
  • the wall 22 is affixed by one end to the top 18 .
  • the length of the wall 22 can be adjusted by an adjustment device 25 .
  • This adjustment device 25 comprises adjusting elements 26 that, by means of a mechanism 27 not shown in further detail and upon actuation of a setting element 28 , can be displaced in the direction of the arrow 29 toward or away from the top 18 .
  • the adjusting elements 26 act on a looping seal 30 configured on the free edge of the wall 22 , said seal cross-sectionally flaring downward to constitute effective protection against stray light.
  • the setting element 28 When the setting element 28 is actuated, the wall can be lowered as needed from the top 18 toward the base 11 to effect sealing or to be moved into a rest position near the top 18 .

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Optical Measuring Cells (AREA)
US11/916,616 2005-06-13 2006-03-04 Thermocycler Abandoned US20080210700A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102005027407A DE102005027407B3 (de) 2005-06-13 2005-06-13 Thermocycler
DE102005027407.2 2005-06-13
PCT/EP2006/001996 WO2006133750A1 (de) 2005-06-13 2006-03-04 Thermocycler

Publications (1)

Publication Number Publication Date
US20080210700A1 true US20080210700A1 (en) 2008-09-04

Family

ID=36570726

Family Applications (1)

Application Number Title Priority Date Filing Date
US11/916,616 Abandoned US20080210700A1 (en) 2005-06-13 2006-03-04 Thermocycler

Country Status (7)

Country Link
US (1) US20080210700A1 (de)
EP (1) EP1890816A1 (de)
JP (1) JP2008543292A (de)
CN (1) CN101198408B (de)
CA (1) CA2610732A1 (de)
DE (1) DE102005027407B3 (de)
WO (1) WO2006133750A1 (de)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8784753B2 (en) 2008-02-15 2014-07-22 Bio-Rad Laboratories, Inc. Thermal cycler with self-adjusting lid
US10605732B2 (en) 2016-01-13 2020-03-31 Institut Dr. Foerster Gmbh & Co. Kg Portable device for detecting explosive substances comprising a device for generating and measuring the emission of an indicator

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102005027555B3 (de) * 2005-06-14 2006-10-05 Eppendorf Ag Thermocycler
EP1961484B1 (de) 2007-02-13 2016-07-20 Eppendorf Ag Deckel für Proben mit homogener Druckanwendung
EP1964609B1 (de) * 2007-02-13 2018-07-18 Eppendorf Ag Verfahren zum Abdecken von Proben mit probengrößenunabhängiger Höhenregulierung
DE102014108143A1 (de) * 2014-06-10 2015-12-10 Kist Europe-Korea Institute of Science and Technologie Europe Forschungsgesellschaft mbh An optical system for detecting fluorescent or luminescent signals of at least two samples

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5602756A (en) * 1990-11-29 1997-02-11 The Perkin-Elmer Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
US6238627B1 (en) * 1998-08-26 2001-05-29 Arqule, Inc. Reaction block and cover
US6657169B2 (en) * 1999-07-30 2003-12-02 Stratagene Apparatus for thermally cycling samples of biological material with substantial temperature uniformity
US20030235517A1 (en) * 2000-09-19 2003-12-25 Holgar Deppe Incubation device
US20040018610A1 (en) * 2002-07-23 2004-01-29 Sandell Donald R. Slip cover for heated platen assembly
US6977722B2 (en) * 2001-06-29 2005-12-20 Meso Scale Technologies, Llc. Assay plates, reader systems and methods for luminescence test measurements

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0523900U (ja) * 1991-09-13 1993-03-30 公和 池亀 食品衛生用インジケータ
CH688987A5 (de) * 1996-11-13 1998-07-15 Doebelin Werner Reaktionskammer fuer die chemische Synthese oder verwandte Anwendungen.
AU759974B2 (en) * 1998-05-16 2003-05-01 Applied Biosystems, Llc Instrument for monitoring polymerase chain reaction of DNA
JP2001337039A (ja) * 2000-05-26 2001-12-07 Dkk Toa Corp 発光検出装置
DE10131687A1 (de) * 2001-06-29 2003-01-16 Eppendorf Ag Vorrichtung zur Durchführung von Nukleinsäure-Amplifikationsreaktionen bei gleichzeitiger Verfolgung der Bildung von Amplifikationsprodukten
JP3811393B2 (ja) * 2001-11-28 2006-08-16 松下電器産業株式会社 蛍光検出装置
DE10205977A1 (de) * 2002-02-08 2003-08-28 Eppendorf Ag Workstation zum Durchführen von chemischen und biologischen Verfahren in Mikrotiterplatten
US20060013736A1 (en) * 2002-04-19 2006-01-19 Blok Herman J System, substrate plate and incubation device for conducting bioassays
US20060057032A1 (en) * 2002-04-19 2006-03-16 Blok Herman J Substrate plate, method and apparatus for manufacturing such a substrate plate, and system for conducting biossays comprising such a substrate plate
DE10232202B4 (de) * 2002-07-16 2005-08-25 H+P Labortechnik Ag Probenbehandlungsstation

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5602756A (en) * 1990-11-29 1997-02-11 The Perkin-Elmer Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
US6238627B1 (en) * 1998-08-26 2001-05-29 Arqule, Inc. Reaction block and cover
US6657169B2 (en) * 1999-07-30 2003-12-02 Stratagene Apparatus for thermally cycling samples of biological material with substantial temperature uniformity
US20040149725A1 (en) * 1999-07-30 2004-08-05 Stratagene Apparatus and method for thermally cycling samples of biological material with substantial temperature uniformity
US20030235517A1 (en) * 2000-09-19 2003-12-25 Holgar Deppe Incubation device
US6977722B2 (en) * 2001-06-29 2005-12-20 Meso Scale Technologies, Llc. Assay plates, reader systems and methods for luminescence test measurements
US20040018610A1 (en) * 2002-07-23 2004-01-29 Sandell Donald R. Slip cover for heated platen assembly

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8784753B2 (en) 2008-02-15 2014-07-22 Bio-Rad Laboratories, Inc. Thermal cycler with self-adjusting lid
US10605732B2 (en) 2016-01-13 2020-03-31 Institut Dr. Foerster Gmbh & Co. Kg Portable device for detecting explosive substances comprising a device for generating and measuring the emission of an indicator

Also Published As

Publication number Publication date
CN101198408A (zh) 2008-06-11
CA2610732A1 (en) 2006-12-21
WO2006133750A1 (de) 2006-12-21
EP1890816A1 (de) 2008-02-27
JP2008543292A (ja) 2008-12-04
CN101198408B (zh) 2010-05-19
DE102005027407B3 (de) 2006-11-09

Similar Documents

Publication Publication Date Title
US7460223B2 (en) Inverted orientation for a microplate
US7417726B2 (en) Normalization of data using controls
US7695688B2 (en) High density plate filler
US8638509B2 (en) Optical camera alignment
US9213042B2 (en) Vacuum assist for a microplate
US20050220675A1 (en) High density plate filler
US20050233363A1 (en) Whole genome expression analysis system
US20100086977A1 (en) Pressure Chamber Clamp Mechanism
US20050232820A1 (en) High density plate filler
US20050226780A1 (en) Manual seal applicator
US20070015289A1 (en) Dispenser array spotting
US20090239293A1 (en) Grooved High Density Plate
US20090197277A1 (en) High Density Plate Filler
US20060024690A1 (en) Normalization of data using controls
US20160325284A1 (en) Pressure Chamber Clamp Mechanism
US20150273469A1 (en) High Density Plate Filler
US20090214811A1 (en) Film Preparation for Seal Applicator
US20050225751A1 (en) Two-piece high density plate
US20080210700A1 (en) Thermocycler
US20050232818A1 (en) Single sheet seal applicator and cartridge
US20050226771A1 (en) High speed microplate transfer
US20060011305A1 (en) Automated seal applicator
US20060029948A1 (en) Sealing cover and dye compatibility selection
US20050233472A1 (en) Spotting high density plate using a banded format
US20050237528A1 (en) Transparent heater for thermocycling

Legal Events

Date Code Title Description
AS Assignment

Owner name: EPPENDORF AG, GERMANY

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:TASCH, HENNER;KRUGER, BERND;REEL/FRAME:020382/0258;SIGNING DATES FROM 20080103 TO 20080114

Owner name: EPPENDORF AG, GERMANY

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:TASCH, HENNER;KRUGER, BERND;SIGNING DATES FROM 20080103 TO 20080114;REEL/FRAME:020382/0258

STCB Information on status: application discontinuation

Free format text: ABANDONED -- AFTER EXAMINER'S ANSWER OR BOARD OF APPEALS DECISION