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US20030145639A1 - Efficient fertilizer and enzyme-assisted method of production - Google Patents

Efficient fertilizer and enzyme-assisted method of production Download PDF

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Publication number
US20030145639A1
US20030145639A1 US09/832,900 US83290001A US2003145639A1 US 20030145639 A1 US20030145639 A1 US 20030145639A1 US 83290001 A US83290001 A US 83290001A US 2003145639 A1 US2003145639 A1 US 2003145639A1
Authority
US
United States
Prior art keywords
mixture
magnesium
enzyme
bacillus
accordance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US09/832,900
Inventor
David Ringelberg
Charles Weiss, Jr.
Philip Malone
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
United States Department of the Army
US Army Corps of Engineers
Original Assignee
United States Department of the Army
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by United States Department of the Army filed Critical United States Department of the Army
Priority to US09/832,900 priority Critical patent/US20030145639A1/en
Assigned to U.S. ARMY CORPS OF ENGINEERS, AS REPRESENTED BY THE SECRETARY OF THE ARMY reassignment U.S. ARMY CORPS OF ENGINEERS, AS REPRESENTED BY THE SECRETARY OF THE ARMY ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: RINGELBERG, DAVID B., MALONE, PHILIP G., WEISS, CHARLES A., JR.
Priority to US10/378,088 priority patent/US6776816B1/en
Publication of US20030145639A1 publication Critical patent/US20030145639A1/en
Abandoned legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D5/00Fertilisers containing magnesium
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00Fertilisers from human or animal excrements, e.g. manure
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/141Feedstock
    • Y02P20/145Feedstock the feedstock being materials of biological origin

Definitions

  • This invention relates to an improved process for producing a slow-release fertilizer from animal waste. Specifically, it relates to a process for composting animal manure with a magnesium-rich compound and selected enzymes that form ammonia from urea and uric acid, and phosphates from phosphate-rich organic compounds. In addition, bacteria selected from guano fertilizer may be incorporated with the enzyme-manure mixture to form magnesium ammonium phosphate.
  • the present invention provides a process for producing slow-release fertilizer from animal manure by composting it with a magnesium-rich compound or a solution of a water-soluble magnesium salt.
  • the animal manure is mixed with a magnesium compound or an aqueous solution of magnesium salt.
  • the temperature of this mixture is maintained at 20-30° C. and the pH is maintained between 7-10.
  • Urease is added to the mixture.
  • the mixture is inoculated with bacteria of the species Bacillus sphaericus, Bacillus globisporus, or Bacillus fusiformis.
  • the mixture is then allowed to incubate for about 14 days to form magnesium ammonium phosphate.
  • phosphatase an enzyme promoting the formation of phosphate from phosphorus-rich organic compounds, is added with the urease enzyme to increase the yield of magnesium-ammonium phosphare.
  • the temperature of this mixture is adjusted to 20-30° C. and maintained in this temperature range.
  • the pH is adjusted to 7-10 by aerating or addition of an acid.
  • Enzymes such as urease are added to the mixture at a dosage of about 0.5 mg of bean sprout urease per liter of mixture. Jack Bean urease Type IX at 62,100 units per gram is suitable.
  • Another suitable material is marketed by Worthington Biochemical Corporation, Lakewood, N.J.
  • the solid enzyme is mixed with 0.1 molar phosphate buffer to bring the enzyme into solution.
  • the mixture is inoculated with bacteria of the species Bacillus sphaericus, Bacillus globisporus, or Bacillus fusiformis which are isolated from guano. Commercial guano can also be used.
  • the mixture is allowed to incubate for about 14 days at 25 to 30° C. to form magesium ammonium phosphate.
  • a phosphatase enzyme is added to the batch along with the urease.
  • an alkaline phosphatase such as bovine alkaline phosphatase (Roche Molecular Biochemicals) is used.
  • This enzyme is added at a dosage of 50 ml of bovine alkaline phosphatase at a concentration of 1000 units/microliter to one liter of manure.
  • the enzyme is blended in with the manure immediately after the addition of the urease and before the addition of the bactertial inoculation

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Fertilizers (AREA)

Abstract

Slow-release fertilizer is produced by composting animal manure with a magnesium-rich compound The temperature of this mixture is maintained at 20-30° C. and the pH is maintained between 7-10. Urease is added to the mixture. Next, the mixture is inoculated with bacteria of the species Bacillus sphaericus, Bacillus globisporus, or Bacillus fusiformis. The mixture is then allowed to incubate for about 14 days to form magnesium ammonium phosphate. Optionally, phosphatase, an enzyme promoting the formation of phosphate from phosphorus-rich organic compounds, is added with the urease enzyme to increase the yield of magnesium-ammonium phosphate.

Description

    GOVERNMENT INTEREST STATEMENT
  • [0001] The invention described herein may be manufactured, licensed and used by or for governmental purposes without the payment of any royalties thereon.
  • I. BACKGROUND OF THE INVENTION
  • 1. Field of the Invention [0002]
  • This invention relates to an improved process for producing a slow-release fertilizer from animal waste. Specifically, it relates to a process for composting animal manure with a magnesium-rich compound and selected enzymes that form ammonia from urea and uric acid, and phosphates from phosphate-rich organic compounds. In addition, bacteria selected from guano fertilizer may be incorporated with the enzyme-manure mixture to form magnesium ammonium phosphate. [0003]
  • 2. Prior Art [0004]
  • The formation of ammonium magnesium phosphate hexahydrate assisted by bacteria has been reported by Nabil Ben mar et al., Chemosphere, vol. 36, No. 3, p 475-481, (1998). The removal of phosphate from supernatant liquors from anaerobically digested sludge by formation of struvite (magnesium ammonium phosphate) is reported by Battistoni et al., Wat. Res., Vol. 31, No. 11, pp. 2925-2929, 1997 The formation of struvite by Azobacter in chemically defined media has been described by Rivadeneira et al., Microbiol. 30, 55-57 (1985). However, there is nothing in the prior art disclosing the use of enzymes in combination with bacteria to produce slow release magnesium-ammonium phosphate fertilizer. The combination of enzymes and bacteria in accordance with this invention accelerates the production of crystalline magnesium ammonium phosphate and increases its yield. The advantages of the present invention over the prior art will be fully appreciated by reading the following description. [0005]
  • II. SUMMARY OF THE INVENTION
  • The present invention provides a process for producing slow-release fertilizer from animal manure by composting it with a magnesium-rich compound or a solution of a water-soluble magnesium salt. [0006]
  • The animal manure is mixed with a magnesium compound or an aqueous solution of magnesium salt. The temperature of this mixture is maintained at 20-30° C. and the pH is maintained between 7-10. Urease is added to the mixture. Next, the mixture is inoculated with bacteria of the species [0007] Bacillus sphaericus, Bacillus globisporus, or Bacillus fusiformis. The mixture is then allowed to incubate for about 14 days to form magnesium ammonium phosphate. Optionally, phosphatase, an enzyme promoting the formation of phosphate from phosphorus-rich organic compounds, is added with the urease enzyme to increase the yield of magnesium-ammonium phosphare.
  • III. DETAILED DESCRIPTION OF THE INVENTION
  • Animal manure is mixed with a low water-solubility of magnesium compound or an aqueous solution of a magnesium salt. Magnesium carbonate, magnesium hydroxide, in the amount of at least 1.7 parts per part of nitrogen in the manure, is added to the animal manure. Highly water-soluble magnesium salts such as magnesium chloride and magnesium sulfate may also be used, but their dosage must be carefully controlled so as to prevent high osmotic pressure of salts which may kill the bacteria. Magnesium sulfate has the additional disadvantage that sulfides or mercaptans may be generated, which have an offensive odor and which may discharge toxic air emissions. Magnesium carbonate and magesium hydroxide provide alkaline buffering to prevent the magnesium ammonium phosphate from being dissolved after it forms. [0008]
  • The temperature of this mixture is adjusted to 20-30° C. and maintained in this temperature range. The pH is adjusted to 7-10 by aerating or addition of an acid. Enzymes such as urease are added to the mixture at a dosage of about 0.5 mg of bean sprout urease per liter of mixture. Jack Bean urease Type IX at 62,100 units per gram is suitable. Another suitable material is marketed by Worthington Biochemical Corporation, Lakewood, N.J. The solid enzyme is mixed with 0.1 molar phosphate buffer to bring the enzyme into solution. [0009]
  • Next, the mixture is inoculated with bacteria of the species [0010] Bacillus sphaericus, Bacillus globisporus, or Bacillus fusiformis which are isolated from guano. Commercial guano can also be used. The mixture is allowed to incubate for about 14 days at 25 to 30° C. to form magesium ammonium phosphate.
  • In an alternate embodiment of this invention, a phosphatase enzyme is added to the batch along with the urease. Typically, an alkaline phosphatase such as bovine alkaline phosphatase (Roche Molecular Biochemicals) is used. This enzyme is added at a dosage of 50 ml of bovine alkaline phosphatase at a concentration of 1000 units/microliter to one liter of manure. The enzyme is blended in with the manure immediately after the addition of the urease and before the addition of the bactertial inoculation [0011]
  • While there have been shown and described what are considered at present to be the preferred embodiments of the present invention, it will be appreciated by those skilled in the art that modifications of such embodiments may be made. It is therefore desired that the invention not be limited to these embodiments and it is intended to cover in the appended claims all such modifications as fall within the true spirit and scope of the invention. [0012]

Claims (6)

We claim:
1. A process for producing slow-release fertilizer from animal waste comprising the steps of:
(a) mixing the animal waste with a water-soluble magnesium salt;
(b) adjusting the pH of the mixture from (a) to between 7 and 10;
(c) adding urease enzyme to the mixture resulting from (b);
(d) inoculating the mixture resulting from (c) with bacteria; and
(e) maintaining the mixture resulting from (d) at 25-30° for about 14 days;
whereby magnesium ammonium phosphate is formed.
2. The process in accordance with claim (1) wherein the water-soluble magnesium is selected from the group consisting of magnesium carbonate and magnesium acetate.
3. The process in accordance with claim (1) wherein the mixture resulting from step (c) is inoculated with bacteria selected from the group consisting of the group consisting of Bacillus sphaericus, Bacillus globisporus, and Bacillus fusiformis.
4. The process in accordance with claim (1) further comprising the step of adding a phosphatase enzyme to the mixture.
5. The process in accordance with claim (1) further comprising the step of adding a urease enzyme to the mixture.
6. The process in accordance with claim (1) wherein the adjustment of pH in step (b) is accomplished by aeration of the mixture resulting from step (a).
US09/832,900 2001-04-12 2001-04-12 Efficient fertilizer and enzyme-assisted method of production Abandoned US20030145639A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US09/832,900 US20030145639A1 (en) 2001-04-12 2001-04-12 Efficient fertilizer and enzyme-assisted method of production
US10/378,088 US6776816B1 (en) 2001-04-12 2003-03-04 Methods for accelerating production of magnesium ammonium phosphate while attaining higher yields thereof and a slow-release fertilizer produced therefrom

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/832,900 US20030145639A1 (en) 2001-04-12 2001-04-12 Efficient fertilizer and enzyme-assisted method of production

Related Child Applications (1)

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US10/378,088 Continuation-In-Part US6776816B1 (en) 2001-04-12 2003-03-04 Methods for accelerating production of magnesium ammonium phosphate while attaining higher yields thereof and a slow-release fertilizer produced therefrom

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US20030145639A1 true US20030145639A1 (en) 2003-08-07

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US10/378,088 Expired - Fee Related US6776816B1 (en) 2001-04-12 2003-03-04 Methods for accelerating production of magnesium ammonium phosphate while attaining higher yields thereof and a slow-release fertilizer produced therefrom

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060188978A1 (en) * 2005-02-24 2006-08-24 Grant Daniel T Products and processes for waste control
US20060186585A1 (en) * 2005-02-22 2006-08-24 Afshin Sadri Systems, methods and apparatus for non-disruptive and non-destructive inspection of metallurgical furnaces and similar vessels
KR100899170B1 (en) 2007-07-12 2009-05-26 경상대학교산학협력단 Shortening fermentation period by composting intermediate product and manufacturing method of high quality organic compost
DE102012000061A1 (en) * 2012-01-03 2013-07-04 Tobias Pickert Recovering magnesium ammonium phosphate as a fertilizer, useful for all toilets and collecting sites of human and animal urine, comprises adding urease enzyme and magnesium chloride to urine in waterless and water-saving urinals
EP2690080A1 (en) 2012-07-27 2014-01-29 Fertiberia, S.A. Fertilisers enriched with a humic-enzimatic solution rich in phosphatase enzymes and their manufacturing process
EP2682378A3 (en) * 2012-07-05 2015-10-14 Wolfgang Tentscher Method and device for processing biomass containing nitrogen
CN109293414A (en) * 2018-11-06 2019-02-01 天津天丰泽田生物科技有限公司 A kind of compound Water soluble fertilizer of rice organic-biological
DE102021117120A1 (en) 2021-07-02 2023-01-05 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung eingetragener Verein Microencapsulation of ammonium phosphate-forming bacteria and its use

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040265266A1 (en) * 2003-04-25 2004-12-30 Michael Augustus Champ Use of magnesium hydroxide and calcium compounds with and without a carrier medium to treat animal waste: to reduce air emissions (including ammonia volatilization) from, retain nutrients from, and manage phosphorous solubility of decaying animal litter, manure, and animal excretions and waste in CAFOs and animal enclousures; to reduce farm nutrient runoff; to extract and bind waste nutrients for fertilizer use; and to reduce air emission of waste-based fertilizers and animal bedding
AU2003902178A0 (en) * 2003-05-07 2003-05-22 Commonwealth Scientific And Industrial Research Organisation Stabilization of waste material
US7384556B2 (en) * 2005-07-19 2008-06-10 The University Of Wyoming Research Corporation Methods of enhancing biodegradation of groundwater contaminants
US8017019B2 (en) * 2007-06-14 2011-09-13 Kansas State University Research Foundation Fluidized bed precipitator with optimized solids settling and solids handling features for use in recovering phosphorus from wastewater
US7604740B2 (en) * 2008-02-01 2009-10-20 Clean Water Services Waste activated sludge stripping to remove internal phosphorus
US8262765B2 (en) * 2008-08-08 2012-09-11 Ajinomoto North America, Inc. Method of preparing a controlled release fertilizer
EP2435385B2 (en) * 2009-05-26 2021-06-16 Compass Minerals Manitoba Inc. High bioavailability phosphorus
DE102012220810B3 (en) * 2012-11-14 2014-02-13 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Process for increased phosphorus recovery from organic residues
GB2524458B (en) 2013-03-11 2020-06-17 Mosaic Co Granulated feed phosphate compsition including feed enzymes
US8691551B1 (en) 2013-03-15 2014-04-08 Ductor Oy Method for recovering phosphorus from organic materials
PL3126507T3 (en) 2014-04-01 2022-02-14 Ductor Oy Biogas process with nutrient recovery
CN104788219A (en) * 2015-04-24 2015-07-22 苏州市新泾村农业基地专业合作社 Biological enzyme organic compound fertilizer for tomatoes
CN104803791A (en) * 2015-04-24 2015-07-29 苏州市新泾村农业基地专业合作社 Biological enzyme organic compound fertilizer for pepper
CN104892187A (en) * 2015-06-10 2015-09-09 苏州市小林农业科技发展有限公司 Biological enzyme organic compound fertilizer for citrus maxima
BR112018071849A2 (en) 2016-05-16 2019-02-19 Arevo Ab solid phase fertilizer composition
US10604433B2 (en) 2017-10-24 2020-03-31 Clean Water Services Emancipative waste activated sludge stripping to remove internal phosphorus (“eWASSTRIP”)

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US6206945B1 (en) 1998-08-10 2001-03-27 Charles Arthur Weiss, Jr. Method of producing artificial guano

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060186585A1 (en) * 2005-02-22 2006-08-24 Afshin Sadri Systems, methods and apparatus for non-disruptive and non-destructive inspection of metallurgical furnaces and similar vessels
US20060188978A1 (en) * 2005-02-24 2006-08-24 Grant Daniel T Products and processes for waste control
KR100899170B1 (en) 2007-07-12 2009-05-26 경상대학교산학협력단 Shortening fermentation period by composting intermediate product and manufacturing method of high quality organic compost
DE102012000061A1 (en) * 2012-01-03 2013-07-04 Tobias Pickert Recovering magnesium ammonium phosphate as a fertilizer, useful for all toilets and collecting sites of human and animal urine, comprises adding urease enzyme and magnesium chloride to urine in waterless and water-saving urinals
EP2682378A3 (en) * 2012-07-05 2015-10-14 Wolfgang Tentscher Method and device for processing biomass containing nitrogen
EP2690080A1 (en) 2012-07-27 2014-01-29 Fertiberia, S.A. Fertilisers enriched with a humic-enzimatic solution rich in phosphatase enzymes and their manufacturing process
CN109293414A (en) * 2018-11-06 2019-02-01 天津天丰泽田生物科技有限公司 A kind of compound Water soluble fertilizer of rice organic-biological
DE102021117120A1 (en) 2021-07-02 2023-01-05 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung eingetragener Verein Microencapsulation of ammonium phosphate-forming bacteria and its use
DE102021117120B4 (en) 2021-07-02 2023-10-19 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung eingetragener Verein Method for producing a phosphorus-containing fertilizer using microcapsules comprising ammonium magnesium phosphate-producing bacteria and a magnesium source

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Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:RINGELBERG, DAVID B.;WEISS, CHARLES A., JR.;MALONE, PHILIP G.;REEL/FRAME:011738/0860;SIGNING DATES FROM 20010320 TO 20010322

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