US20030119853A1

US20030119853A1 - Thiocarboxamide derivatives

Info

Publication number: US20030119853A1
Application number: US10/327,409
Authority: US
Inventors: Sarah Archibald; John Head; Graham Warrellow; John Porter
Original assignee: Individual
Current assignee: Celltech R&D Ltd
Priority date: 1998-03-24
Filing date: 2002-12-20
Publication date: 2003-06-26
Also published as: EP1066271A1; US6521626B1; JP2002507606A; AU5264599A; WO1999048879A1

Abstract

Compounds of formula (1):

wherein:

R¹is a hydrogen atom or an optionally substituted cycloaliphatic, polycycloaliphatic, heterocycloaliphatic; polyheterocycloaliphatic, aromatic, or heteroaromatic group;

Alk¹is an optionally substituted aliphatic or heteroaliphatic chain;

L¹is a linker atom or group;

r and s is each zero or an integer 1;

R²and R³, which may be the same or different, is each a hydrogen or halogen atom or a straight or branched alkyl, haloalkyl, alkoxy, haloalkoxy, hydroxyl or nitro group;

Alk²is a straight or branched alkylene chain;

is zero or an integer 1;

R⁴is a hydrogen atom or a methyl group;

R⁵is a hydrogen atom or a straight or branched alkyl group;

R⁶is a group —(CH₂)_tR⁷in which t is zero or the integer 1 and R⁷is an optionally substituted polycycloaliphatic, heterocycloaliphatic, polyheterocycloaliphatic, aromatic or heteroaromatic group;

R is a carboxylic acid (—CO₂H) or a derivative thereof;

and the salts, solvates and hydrates thereof are described

The compounds are able to inhibit the binding of alpha 4 integrins to their ligands and are of use in the prophylaxis and treatment of immune or inflammatory disorders.

Description

This invention relates to a series of thioamides, to compositions containing them, to processes for their preparation, and to their use in medicine.

Over the last few years it has become increasingly clear that the physical interaction of inflammatory leukocytes with each other and other cells of the body plays an important role in regulating immune and inflammatory responses [Springer, T A Nature, 346. 425, (1990); Springer, T. A. Cell 76, 301, (1994)]. Many of these interactions are mediated by specific cell surface molecules collectively referred to as cell adhesion molecules.

The adhesion molecules have been sub-divided into different groups on the basis of their structure. One family of adhesion molecules which is believed to play a particularly important role in regulating immune and inflammatory responses is the integrin family. This family of cell surface glycoproteins has a typical non-covalently linked heterodimer structure. At least 14 different integrin alpha chains and 8 different integrin beta chains have been identified [Sonnenberg, A Current Topics in Microbiology and Immunology, 184, 7, (1993)]. The members, of the family are typically named according to their heterodimer composition although trivial nomenclature is widespread in this field. Thus the integrin termed α4β1 consists of the integrin alpha 4 chain associated with the integrin beta 1 chain, but is also widely referred to as Very Late Antigen 4 or VLA4. Not all of the potential pairings of integrin alpha and beta chains have yet been observed in nature and the integrin family has been subdivided into a number of subgroups based on the pairings that have been recognised [Sonnenberg, A. ibid].

The importance of cell adhesion molecules in human leukocyte function has been further highlighted by a genetic deficiency disease called Leukocyte Adhesion Deficiency (LAD) in which one of the families of leukocyte integrins is not expressed [Marlin, S. D. et al J. Exp. Med. 164, 855 (1986)]. Patients with this disease have a reduced ability to recruit leukocytes to inflammatory sites and suffer recurrent infections which in extreme cases may be fatal.

The potential to modify adhesion molecule function in such a way as to beneficially modulate immune and inflammatory responses has been extensively investigated in animal models using specific monoclonal antibodies that block various functions of these molecules [e.g. Issekutz, T. B. J. Immunol. 3394, (1992); Li, Z. et al Am. J. Physiol. 263, L723, (1992); Binns, R. M. et al J. Immunol. 157, 4094, (1996)]. A number of monoclonal antibodies which block adhesion molecule function are currently being investigated for their therapeutic potential in human disease.

One particular integrin subgroup of interest involves the a4 chain which can pair with two different beta chains 01 and 07 [Sonnenberg, A. ibid]. The α4β1 pairing occurs on many circulating leukocytes (for example lymphocytes, monocytes and eosinophils) although it is absent or only present at low levels on circulating neutrophils. α4β1 binds to an adhesion molecule (Vascular Cell Adhesion Molecule-1 also known as VCAM-1) frequently up-regulated on endothelial cells at sites of inflammation [Osborne, L. Cell, 62, 3, (1990)]. The molecule has also been shown to bind to at least three sites in the matrix molecule fibronectin [Humphries, M. J. et al. Ciba Foundation Symposium, 189,177, (1995)]. Based on data obtained with monoclonal antibodies in animal models it is believed that the interaction between α4β1 and ligands on other cells and the extracellular matrix plays an important role in leukocyte migration and activation [Yednock, T. A. et al, Nature, 356, 63, (1992); Podolsky, D. K. et al. J. Clin. Invest. 92, 373, (1993); Abraham, W. M. et al. J. Clin. Invest. 93, 776, (1994)].

The integrin generated by the pairing of α4 and β7 has been termed LPAM-1 [Holzmann, B and Weissman, I. EMBO J. 8, 1735, (1989)] and like α4β1 binds to VCAM-1 and fibronectin. In addition, α4β7 binds to an adhesion molecule believed to be involved in the homing of leukocytes to mucosal tissue termed MAdCAM-1 [Berlin, C. et al, Cell, 74, 185, (1993)]. The interaction between α4β7 and MAdCAM-1 may also be important at sites of inflammation outside of mucosal tissue [Yang, X-D. et al, PNAS, 91, 12604 (1994)].

Regions of the peptide sequence recognised by α4β1 and α4β7 when they bind to their ligands have been identified. α4β1 seems to recognise LDV, IDA or REDV peptide sequences in fibronectin and a QIDSP sequence in VCAM-1 [Humphries, M. J. et al, ibid] whilst α4β7 recognises a LDT sequence in MAdCAM-1 [Briskin, M. J. et al, J. Immunol. 156, 719, (1996)]. There have been several reports of inhibitors of these interactions being designed from modifications of these short peptide sequences [Cardarelli, P. M. et al J. Biol. Chem. 2, 18668, (1994); Shroff, H. N. Bioorganic. Med. Chem. Lett. 6, 2495, (1996); Vanderslice, P. J. Immunol. 158, 1710, (1997)]. It has also been reported that a short peptide sequence derived from the α4β1 binding site in fibronectin can inhibit a contact hypersensitivity reaction in a trinitrochlorobenzene sensitised mouse [Ferguson, T. A. et al, PNAS 88, 8072, (1991)].

Since the alpha 4 subgroup of integrins are predominantly expressed on leukocytes their inhibition can be expected to be beneficial in a number of immune or inflammatory disease states. However, because of the ubiquitous distribution and wide range of functions performed by other members of the integrin family it is very important to be able to identify selective inhibitors of the alpha 4 subgroup.

We have now found a group of compounds which are potent and selective inhibitors of α4 integrins. Members of the group are able to inhibit α4 integrins such as α4β1 and/or α4β7 at concentrations at which they generally have no or minimal inhibitory action on a integrins of other subgroups. The compounds are thus of use in medicine, for example in the prophylaxis and treatment of immune or inflammatory disorders as described hereinafter.

Thus according to one aspect of the invention we provide a compound of formula (1)

wherein

R ¹is a hydrogen atom or an optionally substituted cycloaliphatic, polycycloaliphatic, heterocycloaliphatic, polyheterocycloaliphatic, aromatic or heteroaromatic group;

Alk ¹is an optionally substituted aliphatic or heteroaliphatic chain,

L ¹is a linker atom or group;

r and s is each zero or an integer 1;

R ²and R³, which may be the same or different, is each a hydrogen or halogen atom or a straight or branched alkyl, haloalkyl, alkoxy, haloalkoxy, hydroxyl or nitro group;

Alk ²is a straight or branched alkylene chain;

m is zero or an integer 1;

R ⁴is a hydrogen atom or a methyl group;

R ⁵is a hydrogen atom or a straight or branched alkyl group;

R ⁶is a group —(CH₂)_tR⁷in which t is zero or the integer 1 and R⁷is an optionally substituted polycycloaliphatic, heterocycloaliphatic, polyheterocycloaliphatic, aromatic or heteroaromatic group;

R is a carboxylic acid (—CO ₂H) or a derivative thereof;

and the salts, solvates and hydrates thereof.

It will be appreciated that compounds of formula (1) may have one or more chiral centres. Where one or more chiral centres is present, enantiomers or diastereomers may exist, and the invention is to be understood to extend to all such enantiomers, diasteromers and mixtures thereof, including racemates. Formula (1) and the formulae hereinafter are intended to represent all individual isomers and mixtures thereof, unless stated or shown otherwise.

In the compounds of formula (1), derivatives of the carboxylic acid group R include carboxylic acid esters and amides. Particular esters and amides include those —CO ₂Alk⁵, —CONH₂, —CONHR¹²and —CON[R¹²]₂groups described below in relation to the group R⁷.

Alk ²in the compounds of the invention may be for example a straight or branched C_1-3alkylene chain. Particular examples include —CH₂—, —CH(CH₃)— and —(CH₂)₂—.

When R ⁵in the compounds of formula (1) is a straight or branched alkyl group it may be a straight or branched C_1-6alkyl group, e.g. a C_1-3alkyl group such as a methyl or ethyl group.

When in the compounds of the invention L ¹is present as a linker atom or group it may be any divalent linking atom or group. Particular examples include —O— or —S— atoms or —C(O)—, —C(O)O—, —C(S)—, —S(O)—, —S(O)₂—, —N(R⁸)—[where R⁸is a hydrogen atom or an optionally substituted alkyl group], —CON(R⁸)—, —OC(O)N(R⁸)—, —CSN(R⁸)—, —N(R⁸)CO—, —N(R⁸)C(O)O—, —N(R⁸)CS—, —S(O)₂N(R⁸)—, —N(R⁸)S(O)₂—, —N(R⁸)CSN(R⁸)—, or —N(R⁸)SO₂N(R⁸)— groups. Where the linker group contains two R⁸substituents, these may be the same or different.

When Alk ¹in compounds of formula (1) is an optionally substituted aliphatic chain it may be an optionally substituted C_1-10aliphatic chain. Particular examples include optionally substituted straight or branched chain C_1-6alkyl, C_2-6alkenyl, or C_2-6alkynyl chains.

Heteroaliphatic chains represented by Alk ¹include the aliphatic chains just described but with each chain additionally containing one, two, three or four heteroatoms or heteroatom-containing groups. Particular heteroatoms or groups include atoms or groups L²where L²is as defined above for L¹when L¹is a linker atom or group. Each L²atom or group may interrupt the aliphatic chain, or may be positioned at its terminal carbon atom to connect the chain to an adjoining atom or group.

Particular examples of aliphatic chains represented by Alk ¹include optionally substituted —CH₂—, —CH₂CH₂—, —CH(CH₃)—, —C(CH₃)₂, —(CH₂)₂CH₂—, —CH(CH₃)CH₂—, —(CH₂)₃CH₂—, —CH(CH₃)CH₂CH₂—, —CH₂CH(CH₃)CH₂—, —C(CH₃)₂CH₂—, —(CH₂)₄CH₂—, —(CH₂)₅CH₂—, —CHCH—, —CHCHCH₂—, —CH₂CHCH—, —CHCHCH₂CH₂—, —CH₂CHCHCH₂—, —(CH₂)₂CHCH—, —CC—, —CCCH₂—, —CH₂CC—, —CCCH₂CH₂—, —CH₂CCCH₂—, or —(CH₂)₂CC— chains. Where appropriate each of said chains may be optionally interrupted by one or two atoms and/or groups L²to form an optionally substituted heteroaliphatic chain. Particular examples include optionally substituted —L²CH₂—, —CH₂L²CH₂—, —L²(CH₂)₂—, —CH₂L²(CH₂)₂—, —(CH₂)₂L²CH₂—, —L²(CH₂)₃— and —(CH₂)₂L²(CH₂)₂— chains.

The optional substituents which may be present on aliphatic or heteroaliphatic chains represented by Alk ¹include one, two, three or more substituents where each substituent may be the same or different and is selected from halogen atoms, e.g. fluorine, chlorine, bromine or iodine atoms, or C_1-6alkoxy, e.g. methoxy or ethoxy, thiol, C_1-6alkylthio e.g. methylthio or ethylthio, amino or substituted amino groups. Substituted amino groups include —NHR⁹and —N(R⁹)₂groups where R⁹is an optionally substituted straight or branched alkyl group as defined above for R⁵. Where two R⁹groups are present these may be the same or different. Particular examples of substituted chains represented by Alk¹include those specific chains just described substituted by one, two, or three halogen atoms such as fluorine atoms, for example chains of the type —CH(CF₃)—, —C(CF₃)₂— —CH₂CH(CF₃)—, —CH₂C(CF₃)₂—, —CH(CF₃)— and —C(CF₃)₂CH₂.

Optionally substituted heterocycloaliphatic groups represented by R ⁷include optionally substituted C_3-10heterocycloaliphatic groups. Particular examples include optionally substituted C_3-10heterocycloalkyl, e.g. C_3-7heterocycloalkyl or C_3-10heterocycloalkenyl, e.g. C_3-7hetercycloalkenyl groups, each of said groups containing one, two, three or four heteroatoms or heteroatom-containing groups L²as just defined.

Optionally substituted polycycloaliphatic groups represented by R ⁷include optionally substitued C_7-10bi- or tricycloalkyl or C_7-10bi- or tricycloalkenyl groups. Optionally substituted polyheterocycloaliphatic groups represented by R⁷include the optionally substituted polycycloalkyl groups just described, but with each group additionally containing one, two, three or four L³atoms or groups.

Particular examples of R ⁷polycycloaliphatic, heterocycloaliphatic and polyheterocycloaliphatic groups include optionally substituted adamantyl, norbornyl, norbornenyl, tetrahydrofuranyl, pyrroline, e.g. 2- or 3-pyrrolinyl, pyrrolidinyl, pyrrolidinone, oxazolidinyl, oxazolidinone, dioxolanyl, e.g. 1,3-dioxolanyl, imidazolinyl, e.g. 2-imidazolinyl, imidazolidinyl, pyrazolinyl, e.g. 2-pyrazolinyl, pyrazolidinyl, pyranyl, e.g. 2- or 4-pyranyl, piperidinyl, piperidinone, 1,4-dioxanyl, morpholinyl, morpholinone, 1,4-dithianyl, thiomorpholinyl, piperazinyl, 1,3,5-trithianyl, oxazinyl, e.g. 2H-1,3-, 6H-1,3-6H-1,2-, 2H-1,2- or 4H-1,4-oxazinyl, 1,2,5-oxathiazinyl, isoxazinyl, e.g. o- or p-isoxazinyl, oxathiazinyl, e.g. 1,2,5 or 1,2,6-oxathiazinyl, or 1,3,5,-oxadiazinyl groups.

The optional substituents which may be present on the R ⁷polycycloaliphatic, heterocycloaliphatic or polyheterocycloaliphatic groups include one, two, three or more substituents selected from halogen atoms, e.g. fluorine, chlorine, bromine or iodine atoms, or hydroxyl, C_1-6alkoxy, e.g. methoxy or ethoxy, thiol, C_1-6alkylthio e.g. methylthio or ethylthio, amino or substituted amino groups. Substituted amino groups include —NHR⁹and —N(R⁹)₂groups where R⁹is as defined above. Additionally, when R⁷is a heterocycloaliphatic group containing one or more nitrogen atoms each nitrogen atom may be optionally substituted by a group —(L³)_p(Alk³)_qR¹⁰in which L³is —C(O)—, —C(O)O—, —C(S)—, —S(O)₂—, —CON(R⁸)—, —CSN(R⁸)—, —SON(R⁸)— or SO₂N(R⁸)—; p is zero or an integer 1; Alk³is an optionally substituted aliphatic or heteroaliphatic chain; q is zero or an integer 1; and R¹⁰is a hydrogen atom or an optionally substituted cycloaliphatic, heterocycloaliphatic, polycycloaliphatic, polyheterocyclo-aliphatic, aromatic or heteroaromatic group.

Optionally substituted aliphatic or heteroaliphatic chains represented by Alk ³include those optionally substituted chains described above for Alk¹. Optionally substituted cycloaliphatic, heterocycloaliphatic, polycyloaliphatic or polyheterocycloaliphatic groups represented by R¹⁰include those groups just described for R⁷. Optional substituents which may be present on these groups include those described above in relation to Alk¹aliphatic and heteroaliphatic chains.

Optionally substituted aromatic or heteroaromatic groups represented by R ¹⁰include those aromatic and heteroaromatic groups generally and specifically described below for R⁷.

In the compounds of formula (1), optionally substituted aromatic groups represented by the group R ⁷and/or R¹⁰nclude for example optionally substituted monocyclic or bicyclic fused ring C_6-12aromatic groups, such as optionally substituted phenyl, 1- or 2-naphthyl, 1- or 2-tetrahydronaphthyl, indanyl or indenyl groups.

Optionally substituted heteroaromatic groups, represented by the group R ⁷and/or R¹⁰in compounds of formula (1) include for example optionally substituted C_1-9heteroaromatic groups containing for example one, two, three or four heteroatoms selected from oxygen, sulphur or nitrogen atoms. In general, the heteroaromatic groups may be for example monocyclic or bicyclic fused ring heteroaromatic groups. Monocyclic heteroaromatic groups include for example five- or six-membered heteroaromatic groups containing one, two, three or four heteroatoms selected from oxygen, sulphur or nitrogen atoms. Bicyclic heteroaromatic groups include for example nine- to thirteen-membered fused-ring heteroaromatic groups containing one, two or more heteroatoms selected from oxygen, sulphur or nitrogen atoms.

Particular examples of heteroaromatic groups of these types include optionally substituted pyrrolyl, furyl, thienyl, imidazolyl, N-methylimidazolyl, N-ethylimidazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, pyrazolyl, 1,2,3-triazolyl, 1,2,4-triazolyl, 1,2,3-oxadiazolyl, 1,2,4-oxadiazolyl, 1,2,5-oxadiazolyl, 1,3,4-oxadiazolyl, 1,3,4-thiadiazole, pyridyl, pyrimidinyl, pyridazinyl, pyrazinyl, 1,3,5-triazinyl, 1,2,4-triazinyl, 1,2,3-triazinyl, benzofuryl, [2,3-dihydro]benzofuryl, isobenzofuryl, benzothienyl, benzotriazolyl, indolyl, isoindolyl, benzimidazolyl, imidazo[1,2-a]pyridyl, benzothiazolyl, benzoxazolyl, benzopyranyl, [3, 4-dihydro]benzopyranyl, quinazolinyl, naphthyridinyl, pyrido[3,4-b]pyridyl, pyrido[3,2-b]pyridyl, pyrido[4,3-b]pyridyl, quinolinyl, isoquinolinyl, tetrazolyl, 5,6,7,8-tetrahydroquinolinyl, 5,6,7,8-tetrahydroisoquinolinyl, and imidyl, e.g. succinimidyl, phthalimidyl, or naphthalimidyl such as 1,8-naphthalimidyl.

Optional substituents which may be present on the aromatic or heteroaromatic groups represented by R ⁷and/or R¹⁰include one, two, three or more substituents, each selected from an atom or group R¹¹in which R¹¹is —R^11aor -Alk⁴(R^11a)_m, where R^11ais a halogen atom, or an amino (—NH₂), substituted amino, nitro, cyano, amidino, hydroxyl (—OH), substituted hydroxyl, formyl, carboxyl (—CO₂H), esterified carboxyl, thiol (—SH), substituted thiol, —COR¹²[where R¹²is an -Alk⁴(R^11a)_m, aryl or heteroaryl group], —CSR¹², —SO₃H, —SO₂R¹², —SO₂NH₂, —SO₂NHR¹²SO₂N(R¹²)₂, —CONH₂, —CSNH₂, —CONHR¹², —CSNHR¹², —CON[R¹²]₂, —CSN(R¹²)₂, —N(R⁸)SO₂R¹², —N(SO₂R¹²)₂, —N(R⁸)SO₂NH₂, —N(R⁸)SO₂NHR¹², —N(R⁸)SO₂N(R¹²)₂, —N(R⁸)COR¹², —N(R⁸)CON(R¹²)₂, —N(R⁸)CSN(R¹²)₂, —N(R⁸)CSR¹², —N(R⁸)C(O)OR¹², —SO₂NHet¹[where —NHet¹is an optionally substituted C_5-7cyclicamino group optionally containing one or more other —O— or —S— atoms or —N(R⁸)—, —C(O)— or —C(S)— groups], —CONHet¹, —CSNHet¹, —N(R⁸)SO₂NHet¹, —N(R⁸)CONHet¹, —N(R⁸)CSNHet¹, —SO₂N(R⁸)Het²[where Het²is an optionally substituted monocyclic C_5-7carbocyclic group optionally containing one or more —O— or —S— atoms or —N(R⁸)—, —C(O)— or —C(S)— groups], —CON(R⁸)Het², —CSN(R⁸)Het², —N(R⁸)CON(R⁸)Het², —N(R⁸)CSN(R⁸)Het², aryl or heteroaryl group; Alk⁴is a straight or branched C_1-6alkylene, C_2-6alkenylene or C_2-6alkynylene chain, optionally interrupted by one, two or three —O— or —S— atoms or —S(O)n [where n is an integer 1 or 2] or —N(R¹³)— groups [where R¹³is a hydrogen atom or C_1-6alkyl, e.g. methyl or ethyl group]; and m is zero or an integer 1, 2 or 3. It will be appreciated that when two R⁸or R¹²groups are present in one of the above substituents, the R⁸or R¹²groups may be the same or different.

When in the group Alk ⁴(R^11a)_mm is an integer 1, 2 or 3, it is to be understood that the substituent or substituents R^11amay be present on any suitable carbon atom in -Alk⁴. Where more than one R^11asubstituent is present these may be the same or different and may be present on the same or different atom in -Alk⁴. Clearly, when m is zero and no substituent R^11ais present the alkylene, alkenylene or alkynylene chain represented by Alk⁴becomes an alkyl, alkenyl or alkynyl group.

When R ^11ais a substituted amino group it may be for example a group —NHR¹²[where R¹²is as defined above] or a group —N(R¹²)₂wherein each R¹²group is the same or different.

When R ^11ais a halogen atom it may be for example a fluorine, chlorine, bromine, or iodine atom.

When R ^11ais a substituted hydroxyl or substituted thiol group it may be for example a group —OR¹²or a —SR¹²or —SC(═NH)NH₂group respectively.

Esterified carboxyl groups represented by the group R ^11ainclude groups of formula —CO₂Alk⁵wherein Alk⁵is a straight or branched, optionally substituted C_1-8alkyl group such as a methyl, ethyl, n-propyl, i-propyl, n-butyl, i-butyl, s-butyl or t-butyl group; a C_6-12arylC_1-8alkyl group such as an optionally substituted benzyl, phenylethyl, phenylpropyl, 1-naphthylmethyl or 2-naphthylmethyl group; a C_6-12aryl group such as an optionally substituted phenyl, 1-naphthyl or 2-naphthyl group; a C_6-12aryloxyC_1-8alkyl group such as an optionally substituted phenyloxymethyl, phenyloxyethyl, 1-naphthyl-oxymethyl, or 2-naphthyloxymethyl group; an optionally substituted C_1-8alkanoyloxyC_1-8alkyl group, such as a pivaloyloxymethyl, propionyloxyethyl or propionyloxypropyl group; or a C_6-12aroyloxyC_1-8alkyl group such as an optionally substituted benzoyloxyethyl or benzoyloxypropyl group. Optional substituents present on the Alk⁵group include R^11asubstituents described above.

When Alk ⁴is present in or as a substituent it may be for example a methylene, ethylene, n-propylene, i-propylene, n-butylene, i-butylene, s-butylene, t-butylene, ethenylene, 2-propenylene, 2-butenylene, 3-butenylene, ethynylene, 2-propynylene, 2-butynylene or 3-butynylene chain, optionally interrupted by one, two, or three —O— or —S—, atoms or —S(O)—, —S(O)₂— or —N(R⁸)— groups.

Aryl or heteroaryl groups represented by the groups R ^11aor R¹²include mono- or bicyclic optionally substituted C_6-12aromatic or C_1-9heteroaromatic groups as described above for the group R⁷. The aromatic and heteroaromatic groups may be attached to the remainder of the compound of formula (1) by any carbon or hetero e.g. nitrogen atom as appropriate.

When —NHet ¹or —Het²forms part of a substituent R⁷each may be for example an optionally substituted pyrrolidinyl, pyrazolidinyl, piperazinyl, morpholinyl, thiomorpholinyl, piperidinyl or thiazolidinyl group. Additionally Het²may represent for example, an optionally substituted cyclopentyl or cyclohexyl group. Optional substituents which may be present on —NHet¹or -Het²include those substituents described above in relation to Alk¹chains.

Particularly useful atoms or groups represented by R ¹¹include fluorine, chlorine, bromine or iodine atoms, or C_1-6alkyl, e.g. methyl, ethyl, n-propyl, i-propyl, n-butyl or t-butyl, optionally substituted phenyl, pyridyl, pyrrolyl, furyl, thiazolyl, or thienyl, C_1-6alkylamino, e.g. methylamino or ethylamino, C_1-6hydroxyalkyl, e.g. hydroxymethyl or hydroxyethyl, carboxyC_1-6alkyl, e.g. carboxyethyl, C_1-6alkylthio e.g. methylthio or ethylthio, carboxyC_1-6alkylthio, e.g. carboxymethylthio, 2-carboxyethylthio or 3-carboxypropylthio, C_1-6alkoxy, e.g. methoxy or ethoxy, hydroxyC_1-6alkoxy, e.g. 2-hydroxyethoxy, optionally substituted phenoxy, pyridyloxy, thiazolyoxy, phenylthio or pyridylthio, C_5-7cycloalkoxy, e.g. cyclopentyloxy, haloC_1-6alkyl, e.g. trifluoromethyl, haloC_1-6alkoxy, e.g. trifluoromethoxy, C_1-6alkylamino, e.g. methylamino or ethylamino, amino (—NH₂), aminoC_1-6alkyl, e.g. aminomethyl or aminoethyl, C_1-6dialkylamino, e.g. dimethylamino or diethylamino, C_1-6alkylaminoC_1-6alkyl, e.g. ethylaminoethyl, C_1-6dialkylaminoC_1-6alkyl, e.g. diethylaminoethyl, aminoC_1-6alkoxy, e.g. aminoethoxy, C_1-6alkylaminoC_1-6alkoxy, e.g. methylaminoethoxy, C_1-6dialkylaminoC_1-6alkoxy, e.g. dimethylaminoethoxy, diethylaminoethoxy, isopropylaminoethoxy, or dimethylaminopropoxy, imido, such as phthalimido or naphthalimido, e.g. 1,8-naphthalimido, nitro, cyano, amidino, hydroxyl (—OH), formyl [HC(O)—], carboxyl (—CO₂H), —CO₂Alk⁵[where Alk⁵is as defined above], C_1-6alkanoyl e.g. acetyl, optionally substituted benzoyl, thiol (—SH), thioC_1-6alkyl, e.g. thiomethyl or thioethyl, —SC(═NH)NH₂, sulphonyl (—SO₃H), C_1-6alkylsulphonyl, e.g. methylsulphonyl, aminosulphonyl (—SO₂NH₂), C_1-6alkylaminosulphonyl, e.g. methylaminosulphonyl or ethylaminosulphonyl, C_1-6dialkylaminosulphonyl, e.g. dimethylaminosulphonyl or diethylaminosulphonyl, phenylaminosulphonyl, carboxamido (—CONH₂), C_1-6alkylaminocarbonyl, e.g. methylaminocarbonyl or ethylaminocarbonyl, —C_1-6dialkylaminocarbonyl, e.g. dimethylaminocarbonyl or diethylaminocarbonyl, aminoC_1-6alkylaminocarbonyl, e.g. aminoethylaminocarbonyl, C_1-6dialkylaminoC_1-6alkylaminocarbonyl, e.g. diethylaminoethylaminocarbonyl, aminocarbonylamino, C_1-6alkylaminocarbonylamino, e.g. methylaminocarbonylamino or ethylaminocarbonylamino, C_1-6dialkylaminocarbonylamino, e.g. dimethylaminocarbonylamino or diethylaminocarbonylamino, C_1-6alkylaminocabonylC_1-6alkylamino, e.g. methylaminocarbonylmethylamino, am inothiocarbonylamino, C_1-6alkylaminothiocarbonylamino, e.g. methylaminothiocarbonylamino or ethylaminothiocarbonylamino, C_1-6dialkylaminothiocarbonylamino, e.g. dimethylaminothiocarbonylamino or diethylaminothiocarbonylamino, C_1-6alkylaminothiocarbonylC_1-6alkylamino, e.g. ethylaminothiocarbonylmethylamino, —CONHC(═NH)NH₂, C_1-6alkylsulphonylamino, e.g. methylsulphonylamino or ethylsulphonylamino, C_1-6dialkylsulphonylamino, e.g. dimethylsulphonylamino or diethylsulphonylamino, optionally substituted phenylsulphonylamino, aminosulphonylamino (—NHSO₂NH₂), C_1-6alkylaminosulphonylamino, e.g. methylaminosulphonyl-amino or ethylaminosulphonylamino, C_1-6dialkylaminosulphonylamino, e.g. dimethylaminosulphonylamino or diethylaminosulphonylamino, optionally substituted morpholinesulphonylamino or morpholinesulphonylC_1-6alkylamino, optionally substituted phenylaminosulphonylamino, C_1-6alkanoylamino, e.g. acetylamino, aminoC_1-6alkanoylamino e.g. aminoacetylamino, C_1-6dialkylaminoC_1-6alkanoylamino, e.g. dimethylaminoacetylamino, C_1-6alkanoylaminoC_1-6alkyl, e.g. acetylaminomethyl, C_1-6alkanoylaminoC_1-6alkylamino, e.g. acetamidoethylamino, C_1-6alkoxycarbonylamino, e.g. methoxycarbonylamino, ethoxycarbonylamino or t-butoxycarbonylamino or optionally substituted benzyloxy, pyridylmethoxy, thiazolylmethoxy, benzyloxycarbonylamino, benzyloxycarbonylaminoC_1-6alkyl e.g. benzyloxycarbonylaminoethyl, benzothio, pyridylmethylthio or thiazolylmethylthio groups.

Where desired, two R ¹¹substituents may be linked together to form a cyclic group such as a cyclic ether, e.g. a C_1-6alkylenedioxy group such as methylenedioxy or ethylenedioxy.

It will be appreciated that where two or more R ¹¹substituents are present, these need not necessarily be the same atoms and/or groups. In general, the substituent(s) may be present at any available ring position in the aromatic or heteroaromatic group represented by R⁷.

Alkyl groups represented by the groups R ²or R³in compounds of the invention include for example straight or branched C_1-6alkyl groups such as methyl, ethyl, n-propyl, i-propyl, n-butyl, i-butyl, s-butyl or t-butyl groups. Alkoxy groups represented by the groups R²or R³include straight or branched C_1-6alkoxy groups such as methoxy or ethoxy groups. Halogen atoms represented by the groups R²or R³include for example fluorine, chlorine, bromine or iodine atoms. When R²and/or R³is a haloalkyl or haloalkoxy group it may be for example a haloC_1-6alkyl or haloC_1-6alkoxy group containing one, two or three halogen atoms selected from fluorine, chlorine, bromine or iodine atoms. Particular examples of groups of this type include —CF₃, —OCF₃, —CCl₃, —OCCl₃, —CHF₂, —OCHF₂, —CHCl₂, —OCHCl₂, —CH₂F, —OCH₂F, —CH₂Cl and —OCH₂Cl groups.

The presence of certain substituents in the compounds of formula (1) may enable salts of the compounds to be formed. Suitable salts include pharmaceutically acceptable salts, for example acid addition salts derived from inorganic or organic acids, and salts derived from inorganic and organic bases.

Acid addition salts include hydrochlorides, hydrobromides, hydroiodides, alkylsulphonates, e.g. methanesulphonates, ethanesulphonates, or isethionates, arylsulphonates, e.g. p-toluenesulphonates, besylates or napsylates, phosphates, sulphates, hydrogen sulphates, acetates, trifluoroacetates, propionates, citrates, maleates, fumarates, malonates, succinates, lactates, oxalates, tartrates and benzoates.

Salts derived from inorganic or organic bases include alkali metal salts such as sodium or potassium salts, alkaline earth metal salts such as magnesium or calcium salts, and organic amine salts such as morpholine, piperidine, dimethylamine or diethylamine salts.

Particularly useful salts of compounds according to the invention include pharmaceutically acceptable salts, especially acid addition pharmaceutically acceptable salts.

Generally in the compounds of the invention the group R is preferably a —CO ₂H group.

Alk ²in compounds of formula (1) is preferably a —CH₂— chain and m is preferably an integer 1.

R ⁴and R⁵in compounds of the invention is each preferably a hydrogen atom.

In general in compounds of formula (1) -Alk ¹L¹- is preferably —CH₂O— or —CON(R⁸)—, particularly —CONH—.

The group R ¹in compounds of formula (1) is preferably an optionally substituted aromatic or heteroaromatic group. Particularly useful groups of these types include optionally substitued phenyl, pyridyl or pyrimidinyl groups.

Particularly useful classes of compounds according to the invention are those wherein R ⁶is a group —R⁷[i.e. where t is zero] where R⁷is an optionally substituted heterocycloaliphatic, aromatic or heteroaromatic group as defined herein. Particularly useful groups of this type include optionally substituted C_5-7heterocycloaliphatic, especially optionally substituted pyrrolidinyl or thiazolidinyl, optionally substituted phenyl and optionally substituted C_5-7heteroaromatic, especially optionally substituted pyridinyl groups. Optional substituents on these groups include in particular R¹¹atoms or groups where the group is an aromatic or heteroaromatic group and -(L³)_p(Alk³)_qR¹⁰groups as described earlier where the group is a nitrogen-containing heterocycloaliphatic group such as a pyrrolidinyl or thiazolidinyl group. Particularly useful -(L³)_p(Alk³)_qR¹⁰groups include those in which L³is a —CO-group. Alk³in these groups is preferably present (i.e. q is preferably an integer 1) and in particular is a —CH₂— chain. Compounds of this type in which R⁶is a hydrogen atom or an optionally substituted aromatic or heteroaromatic group, especially an optionally substituted phenyl, pyridyl or imidazolyl group are particularly preferred.

Compounds according to the invention are potent and selective inhibitors of α4 integrins. The ability of the compounds to act in this way may be simply determined by employing tests such as those described in the Examples hereinafter.

The compounds are of use in modulating cell adhesion and in particular are of use in the prophylaxis and treatment of diseases or disorders involving inflammation in which the extravasation of leukocytes plays a role. The invention extends to such uses and to the use of the compounds for preparing a medicament for treating these diseases and disorders. Particular diseases or disorders of this type include inflammatory arthritis such as rheumatoid arthritis vasculitis or polydermatomyositis, multiple sclerosis, allograft rejection, diabetes, inflammatory dermatoses such as psoriasis or dermatitis, asthma and inflammatory bowel disease.

For the prophylaxis or treatment of disease the compounds according to the invention may be administered as pharmaceutical compositions, and according to a further aspect of the invention we provide a pharmaceutical composition which comprises a compound of formula (1) together with one or more pharmaceutically acceptable carriers, excipients or diluents.

Pharmaceutical compositions according to the invention may take a form suitable for oral, buccal, parenteral, nasal, topical or rectal administration, or a form suitable for administration by inhalation or insufflation.

For oral administration, the pharmaceutical compositions may take the form of, for example, tablets, lozenges or capsules prepared by conventional means with pharmaceutically acceptable excipients such as binding agents (e.g. pregelatinised maize starch, polyvinylpyrrolidone or hydroxypropyl methylcellulose); fillers (e.g. lactose, microcrystalline cellulose or calcium hydrogen phosphate); lubricants (e.g. magnesium stearate, talc or silica); disintegrants (e.g. potato starch or sodium glycollate); or wetting agents (e.g. sodium lauryl sulphate). The tablets may be coated by methods well known in the art. Liquid preparations for oral administration may take the form of, for example, solutions, syrups or suspensions, or they may be presented as a dry product for constitution with water or other suitable vehicle before use. Such liquid preparations may be prepared by conventional means with pharmaceutically acceptable additives such as suspending agents, emulsifying agents, non-aqueous vehicles and preservatives. The preparations may also contain buffer salts, flavouring, colouring and sweetening agents as appropriate.

Preparations for oral administration may be suitably formulated to give controlled release of the active compound.

For buccal administration the compositions may take the form of tablets or lozenges formulated in conventional manner.

The compounds for formula (1) may be formulated for parenteral administration by injection e.g. by bolus injection or infusion. Formulations for injection may be presented in unit dosage form, e.g. in glass ampoule or multi dose containers, e.g. glass vials. The compositions for injection may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilising, preserving and/or dispersing agents. Alternatively, the active ingredient may be in powder form for constitution with a suitable vehicle, e.g. sterile pyrogen-free water, before use.

In addition to the formulations described above, the compounds of formula (1) may also be formulated as a depot preparation. Such long acting formulations may be administered by implantation or by intramuscular injection.

For nasal administration or administration by inhalation, the compounds for use according to the present invention are conveniently delivered in the form of an aerosol spray presentation for pressurised packs or a nebuliser, with the use of suitable propellant, e.g. dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas or mixture of gases.

The compositions may, if desired, be presented in a pack or dispenser device which may contain one or more unit dosage forms containing the active ingredient. The pack or dispensing device may be accompanied by instructions for administration.

The quantity of a compound of the invention required for the prophylaxis or treatment of a particular condition will vary depending on the compound chosen, and the condition of the patient to be treated. In general, however, daily dosages may range from around 100 ng/kg to 100 mg/kg e.g. around 0.01 mg/kg to 40 mg/kg body weight for oral or buccal administration, from around 10 ng/kg to 50 mg/kg body weight for parenteral administration and around 0.05 mg to around 1000 mg e.g. around 0.5 mg to around 1000 mg for nasal administration or administration by inhalation or insufflation.

The compounds of the invention may be prepared by a number of processes as generally described below and more specifically in the Examples hereinafter. In the following process description, the symbols R, R ¹-R⁶, L¹, Alk¹, Alk², m, r and s when used in the formulae depicted are to be understood to represent those groups described above in relation to formula (1) unless otherwise indicated. In the reactions described below, it may be necessary to protect reactive functional groups, for example hydroxy, amino, thio or carboxy groups, where these are desired in the final product, to avoid their unwanted participation in the reactions. Conventional protecting groups may be used in accordance with standard practice [see, for example, Green, T. W. in “Protective Groups in Organic Synthesis”, John Wiley and Sons, 1991]. In some instances, deprotection may be the final step in the synthesis of a compound of formula (1) and the processes according to the invention described hereinafter are to be understood to extend to such removal of protecting groups.

Thus according to a further aspect of the invention, a compound of formula (1) in which R is a —CO ₂H group may be obtained by hydrolysis of an ester of formula (2):

where R ¹³is an alkyl group.

The hydrolysis may be performed using either an acid or a base depending on the nature of R ¹³, for example an organic acid such as trifluoroacetic acid or an inorganic base such as lithium hydroxide optionally in an aqueous organic solvent such as an amide, e.g. a substituted amide such as dimethylformamide, an ether, e.g. a cyclic ether such as tetrahydrofuran or dioxane or an alcohol, e.g. methanol at around ambient temperature. Where desired, mixtures of such solvents may be used.

Intermediate esters of formula (2) may be prepared by treatment of a corresponding ester of formula (3):

with a thiation reagent, such as Lawesson's Reagent, in an anhydrous solvent, for example a cyclic ether such as tetrahydrofuran, at an elevated temperature such as the reflux temperature.

This reaction may not be particularly suitable with starting materials in which other carbonyl groups are present, for example in L ¹and/or R⁶, and which might undesirably participate in the reaction. To avoid this the reaction with the thiation reagent may be performed earlier in the synthesis of the compound of the invention with an intermediate in which other carbonyl groups are absent and any required carbonyl groups then subsequently introduced by for example acylation as generally described hereinafter.

Esters of formula (3) may be prepared by coupling an amine of formula (4):

or a salt thereof with an acid R ⁶CO₂H or an active derivative thereof. Active derivatives of acids include anhydrides, esters and halides.

The coupling reaction may be performed using standard conditions for reactions of this type. Thus for example the reaction may be carried out in a solvent, for example an inert organic solvent such as an amide, e.g. a substituted amide such as dimethylformamide, an ether, e.g. a cyclic ether such as tetrahydrofuran, or a halogenated hydrocarbon, such as dichloromethane, at a low temperature, e.g. around −30° C. to around ambient temperature, optionally in the presence of a base, e.g. an organic base such as an amine, e.g. triethylamine, pyridine, or dimethylaminopyridine, or a cyclic amine, such as N-methylmorpholine.

Where an acid R ⁶CO₂H is used, the reaction may additionally be performed in the presence of a condensing agent, for example a diimide such as 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide or N,N′-dicyclohexylcarbodiimide, advantageously in the presence of a catalyst such as a N-hydroxy compound e.g. a N-hydroxytriazole such as 1-hydroxybenzotriazole. Alternatively, the acid may be reacted with a chloroformate, for example ethylchloroformate, prior to reaction with the amine of formula (3).

The esters of formula (3) and acids R ⁶CO₂H are either known compounds or may be obtained from simpler compounds by one or more standard synthetic methods employing substitution, oxidation, reduction or cleavage reactions. Particular substitution approaches include conventional alkylation, arylation, heteroarylation, acylation, thioacylation, halogenation, sulphonylation, nitration, formylation and coupling procedures. It will be appreciated that these methods may also be used to modify the compounds of formula (1) and the esters or formula (2) where appropriate functional groups exist in these conmpounds.

Thus, for example compounds of the invention and intermediates thereto may be prepared by alkylation, arylation or heteroarylation. For example compounds containing a L ¹H or L³H group may be alkylated or arylated using a reagent R¹(Alk¹)_rX or R¹⁰(Alk³)_qX in which X is a leaving atom or group such as a halogen atom, e.g. a fluorine, bromine, iodine or chlorine atom or a sulphonyloxy group such as an alkylsulphonyloxy, e.g. trifluoromethylsulphonyloxy or arylsulphonyloxy, e.g. p-toluenesulphonyloxy group.

The alkylation or arylation reaction may be carried out in the presence of a base such as a carbonate, e.g. caesium or potassium carbonate, an alkoxide, e.g. potassium t-butoxide, or a hydride, e.g. sodium hydride, in a dipolar aprotic solvent such as an amide, e.g. a substituted amide such as dimethylformamide or an ether, e.g. a cyclic ether such as tetrahydrofuran.

In another example, compounds containing a L ¹H or -L³H group may be functionalised by acylation or thioacylation, for example by reaction with a reagent R¹(Alk¹)_rL¹X [wherein L¹is a —C(O)—, —C(S)—, or —N(R⁸)C(S)— group], R¹⁰(Alk³)_qCOX or R¹⁰(Alk³)_qNHCOX in the presence of a base, such as a hydride, e.g. sodium hydride or an amine, e.g. triethylamine or N-methylmorpholine, in a solvent such as a halogenated hydrocarbon, e.g. dichloromethane or carbon tetrachloride or an amide, e.g. dimethylformamide, at for example ambient temperature, or by reaction with R¹(Alk¹)_rCO₂H or R¹⁰(Alk³)_qCO₂H or an activated derivative thereof, for example as described above for the preparation of esters of formula (3).

In a further example a compound may be obtained by sulphonylation of a compound where R ¹(Alk¹)_r(L¹)s is an —OH group by reaction with a reagent R¹(Alk¹)_rL¹Hal [in which L¹is —S(O)— or —SO₂— and Hal is a halogen atom such as a chlorine atom] in the presence of a base, for example an inorganic base such as sodium hydride in a solvent such as an amide, e.g. a substituted amide such as dimethylformamide at for example ambient temperature.

In another example, a compound where R ¹(Alk¹)_r(L¹)_sis a -L¹H group, may be coupled with a reagent R¹OH (where R¹is other than a hydrogen atom) or R¹Alk¹OH in a solvent such as tetrahydrofuran in the presence of a phosphine, e.g. triphenylphosphine and an activator such as diethyl, diisopropyl- or dimethylazodicarboxylate to yield a compound containing a R¹(Alk¹)_rO— group.

In a further example, ester groups —CO ₂Alk⁵in the compounds may be converted to the corresponding acid [—CO₂H] by acid- or base-catalysed hydrolysis depending on the nature of the group Alk⁵using the reactants and conditions described above for the hydrolysis of esters of formula (2).

In another example, —OR ¹²groups [where R¹²represents an alkyl group such as methyl group] in compounds of formula (1) may be cleaved to the corresponding alcohol —OH by reaction with boron tribromide in a solvent such as a halogenated hydrocarbon, e.g. dichloromethane at a low temperature, e.g. around −78° C.

Alcohol [—OH] groups may also be obtained by hydrogenation of a corresponding —OCH ₂R¹²group (where R¹²is an aryl group) using a metal catalyst, for example palladium on a support such as carbon in a solvent such as ethanol in the presence of ammonium formate, cyclohexadiene or hydrogen, from around ambient to the reflux temperature. In another example, —OH groups may be generated from the corresponding ester [—CO₂Alk⁵] or aldehyde [—CHO] by reduction, using for example a complex metal hydride such as lithium aluminium hydride or sodium borohydride in a solvent such as methanol.

Aminosulphonylamino [—NHSO ₂NH₂] groups in the compounds may be obtained, in another example, by reaction of a corresponding amine [—NH₂] with sulphamide in the presence of an organic base such as pyridine at an elevated temperature, e.g. the reflux temperature.

In a further example amine (—NH ₂) groups may be alkylated using a reductive alkylation process employing an aldehyde and a borohydride, for example sodium triacetoxyborohyride or sodium cyanoborohydride, in a solvent such as a halogenated hydrocarbon, e.g. dichloromethane, a ketone such as acetone, or an alcohol, e.g. ethanol, where necessary in the presence of an acid such as acetic acid at around ambient temperature.

In a further example, amine [—NH ₂] groups in compounds of formula (1) may be obtained by hydrolysis from a corresponding imide by reaction with hydrazine in a solvent such as an alcohol, e.g. ethanol at ambient temperature.

In another example, a nitro [—NO ₂] group may be reduced to an amine [—NH₂], for example by catalytic hydrogenation using for example hydrogen in the presence of a metal catalyst, for example palladium on a support such as carbon in a solvent such as an ether, e.g. tetrahydrofuran or an alcohol e.g. methanol, or by chemical reduction using for example a metal, e.g. tin or iron, in the presence of an acid such as hydrochloric acid.

Aromatic halogen substituents in the compounds may be subjected to halogen-metal exchange with a base, for example a lithium base such as n-butyl or t-butyl lithium, optionally at a low temperature, e.g. around −78° C., in a solvent such as tetrahydrofuran and then quenched with an electrophile to introduce a desired substituent. Thus, for example, a formyl group may be introduced by using dimethylformamide as the electrophile; a thiomethyl group may be introduced by using dimethyidisulphide as the electrophile.

In another example, sulphur atoms in the compounds, for example when present in a linker group L ¹or L³may be oxidised to the corresponding sulphoxide or sulphone using an oxidising agent such as a peroxy acid, e.g. 3-chloroperoxybenzoic acid, in an inert solvent such as a halogenated hydrocarbon, e.g. dichloromethane, at around ambient temperature.

N-oxides of compounds of formula (1) may be prepared for example by oxidation of the corresponding nitrogen base using an oxidising agent such as hydrogen peroxide in the presence of an acid such as acetic acid, at an elevated temperature, for example around 70° C. to 80° C., or alternatively by reaction with a peracid such as peracetic acid in a solvent, e.g. dichloromethane, at ambient temperature.

Salts of compounds of formula (1) may be prepared by reaction of a compound of formula (1) with an appropriate base in a suit able solvent or mixture of solvents e.g. an organic solvent such as an ether e.g. diethylether, or an alcohol, e.g. ethanol using conventional procedures.

Where it is desired to obtain a particular enantiomer of a compound of formula (1) this may be produced from a corresponding mixture of enantiomers using any suitable conventional procedure for resolving enantiomers.

Thus for example diastereomeric derivatives, e.g. salts, may be produced by reaction of a mixture of enantiomers of formula (1) e.g. a racemate, and an appropriate chiral compound, e.g. a chiral base. The diastereomers may then be separated by any convenient means, for example by crystallisation and the desired enantiomer recovered, e.g. by treatment with an acid in the instance where the diastereomer is a salt.

In another resolution process a racemate of formula (1) may be separated using chiral High Performance Liquid Chromatography. Alternatively, if desired a particular enantiomer may be obtained by using an appropriate chiral intermediate in one of the processes described above.

The following Examples illustrate the invention. All temperatures are in ° C. The following abbreviations are used:



	EDC	1-(3-dimethylaminopropyl)3-ethycarbodiimide;
	DMF	dimethylformamide;
	HOBT	1-hydroxybenzotriazole;
	TFA	trifluoroacetic acid;
	DCM	dichloromethane;
	BOC	tert-butoxycarbonyl;
	MeOH	methanol;
	tyr	tyrosine;
	HetAr	heteroaryl;
	thiopro	thioproline;
	Me	methyl;
	DMSO	dimethylsulphoxide;
	THF	tetrahydrofuran
	NMM	N-methylmorpholine;
	Ph	phenyl;
	EtOAc	ethyl acetate;
	LDA	lithium diisopropylamide
	Ar	aryl;
	pyr	pyridine;
	Bu	butyl

Intermediate 1

N-BOC-D-thioproline-(O-2,6-dichlorobenzyl)-L-tyrosine Methyl Ester

EDC (634 mg, 3.3 mmol) was added to a solution of N-BOC-D-thioproline (699 mg, 3 mmol), (O-2,6-dichlorobenzyl)-L-tyrosine methyl ester hydrochloride (1.17 g, 3 mmol), HOBT (446 mg, 3.3 mmol) and NMM (692 μl, 6.3 mmol) in DCM (30 ml). The mixture was stirred for 4 h at room temperature then diluted with DCM (20 ml) and washed with dilute hydrochloric acid (1 M, 50 ml), saturated NaHCO ₃solution (50 ml) and water (50 ml). The DCM solution was dried (Na₂SO₄) and evaporated under reduced pressure to give the title compound (1.70 g) as a white foam. 5H (DMSO-d₆, 300K) 8.41 (1H, br s, CONH), 7.57-7.43 (3H, m, ArH), 7.15 (2H, d, J 8.5 Hz, ArH), 6.96 (2H, d, J 8.6 Hz, ArH), 5.18 (2H, s, OCH₂Ar), 4.6-4.4 (2H, br m, CHαthiopro+CHαtyr), 4.56 (1H, d, J 8.9 Hz, NCH _AH_BS), 4.23 (1H, d, J 9.0 Hz, NCH_A H _BS), 3.64 (3H, s, CO₂Me), 3.19 (1H, dd, J 7.5, 11.5 Hz, CHCH _AH_BS), 3.04 (1H, dd, J 5.1, 13.7 Hz, CHCH _AH_BAr), 2.85 (1H, dd, J 9.6, 13.7 Hz, CHCH_A B _BAr), 2.7 (1H, br m, CHCH_A H _BS) and 1.33 (9H, br s, tBu); m/z (ESI, 60V) 591 M ⁺+Na).

Intermediate 2

Methyl (2R)-3-{4-[(2,6-dichlorobenzyl)oxy]phenyl}-2-({[(4S)-3-(tert-butoxycarbonyl)-1,3-thiazolan-4-yl]carbothioyl}amino)propanoate

Lawesson's reagent (202 mg, 0.5 mmol) was added to a solution of Intermediate 1 (569 mg, 1 mmol) in THF (10 ml). The suspension was refluxed for 3.5 h to give a yellow solution. The solvent was removed under reduced pressure and the residue purified by column chromatography (SiO ₂; DCM) to give the title compound as a colourless gum (345 mg, 59%). δH (DMSO-d₆, 300K) 10.3 (1H, br s, CSNH), 7.57-7.42 (3H, m, ArH), 7.17 (2H, d, J 8.3 Hz, ArH), 6.96 (2H, d, J 8.6 Hz, ArH), 5.27 (1H, br s, CHαthiapro), 5.18 (2H, s, OCH₂Ar), 4.88 (1H, br s, CHαtyr), 4.65 (1H, d, J 9.1 Hz, NCH _AH_BS) 4.37 (1H, d, J 9.2 Hz, NCH_A H _BS), 3.66 (3H, s, CO₂Me), 3.33-3.27 (1H, m, CHCH _AH_BS), 3.18 (1H., dd, J 5.5, 14.0 Hz, CHCH _AH_BAr), 3.05 (1H, dd, J 9.1, 14.0 Hz, CHCH_A H _BAr), 2.73 (1H, br m, CHCH_A H _BS) and 1.31 (9H, br s, CMe₃); m/z (ESI, 60V) 605 (M ⁺Na).

Intermediate 3

Methyl (2R)-3-{4-[(2,6-dichlorobenzyl)oxy]phenyl}-2-{[(4S)-1,3 thiazolan-4-ylcarbothioyl]amino}propanoate Hydrochloride

Anhydrous HCl gas was bubbled through a solution of Intermediate 2 (324 mg, 0.554 mmol) in EtOAc (10 ml) for one minute. After 30 min the solvent was removed under reduced pressure to give the title compound (292 mg) as a yellow solid. δH (DMSO-d ₆, 300K) 11.19 (1H, br d, J 7.3 Hz, CSNH), 7.57-7.43 (3H, m, ArH), 7.20 (2H, d, J 8.6 Hz, ArH), 6.98 (2H, d, J 8.6 Hz, ArH), 5.21-5.18 (1H, m, CHαtyr), 5.18 (2H, s, OCH₂Ar), 4.65 (1H, br t, J 7.7 Hz, CHαthiopra), 4.33 (1H, d, J 9.6 Hz, NCH _AH_BS), 4.28 (1H, d, J 9.6 Hz, NCH_A H _BS), 3.69 (3H, S, CO₂Me), 3.33 (1H, dd, J 7.1, 11.2 Hz, CHCH _AH_BS), 3.23 (1H, dd, J 5.2, 13.9 Hz, CH _AH_BAr), 3.08 (1H, dd, J 9.8, 13.9 Hz, CH_A H _BAr) and 2.61 (1H, dd, J 8.5, 11.2 Hz, CHCH_A H _BS); m/z (ESI, 60V) 485 (M ⁺+1).

Intermediate 4

Methyl (2R)-2-({[(4S)-3-acetyl-1,3-thiazolan-4-yl]carbothioyl}amino)-3-{4-[(2,6-dichlorobenzyl)oxy]phenyl}propanoate

Acetic anhydride (57 μl, 0.602 mmol) was added to a solution of Intermediate 3 (285 mg, 0.547 mmol) and NMM (60 μl, 0.547 mmol) in DCHM (10 ml). The mixture was stirred at room temperature for 3 h, diluted with DCM (100 ml), washed with dilute HCl (aqueous) (20 ml), dried (Na ₂SO₄) and evaporated under reduced pressure. The residue was purified by column chromatography (SiO₂; EtOAc/hexane, 50:50 to 75:25) to give the title compound as a colourless oil (285 mg). δH (DMSO-d₆, 400K) 9.67 (1H, br s, CSNH), 7.51-7.39 (3H, m, ArH), 7.17 (2H, d, J 8.6 Hz, ArH), 6.97 (2H, d, J 8.6 Hz, ArH), 5.36 (1H;, dd, J 5.8, 8.1 Hz, CHαtyr), 5.26 (2H, s, OCH₂Ar), 5.15 (1H, dd, J 4.6, 7.4 Hz, CHαthiopro), 4.83 (1H, d, J 9.3 Hz, NCH _AH_BS), 4.51 (1H, d, J 9.3 Hz, NCH_A H _BS), 3.68 (3H, S, CO₂Me), 3.39 (1H, dd, J 7.5, 11.7 Hz, CHCH _AH_BS), 3.21 (1H, dd, J 5.8, 14.2 Hz, CH _AH_BAr), 3.13 (1H, dd, J 8.3, 14.2 Hz, CH_A H _BAr), 3.07 (1H, dd, J 4.8, 11.7 Hz, CHCH_A H _BS) and 1.95 (3H, s, NCOCH₃); m/z (ESI, 60V) 527 (M ⁺+1).

EXAMPLE 1

(A) (2R)-2-({[(4S)-3-Acetyl-1,3-thiazolan-4-yl]carbothioyl}amino)-3-{4-[(2,6-dichlorobenzyl)oxy]phenyl}propanoic Acid [0122]
(B) (2R)-2-({[(4R)-3-Acetyl-1,3-thiazolan-4-yl]carbothioyl}amino)-3-{4-[(2,6-dichlorobenzyl)oxy]phenyl}propanoic Acid [0123]
Lithium hydroxide monohydrate (25 mg, 0.58 mmol) was added to a solution of Intermediate 4 (280 mg, 0.531 mmol) in THF (5 ml) and water (5 ml). After 1 h at room temperature the THF was removed under reduced pressure, the aqueous residue acidified and extracted with DCM (2×50 ml). The extracts were dried (Na[0124] ₂SO₄) and evaporated under reduced pressure to give a fluffy white solid (233 mg). Separation by preparative HPLC gave the title compound, diastereoisomer A δH (DMSO-d₆, 390K) 955 (1H, br s, CSNH), 7.51-7.39 (3H, m, ArH), 7.17 (2H, d, J 8.7 Hz, ArH), 6.96 (2H, d, J 8.7ArH), 5.26 (2H, s, OCH₂Ar), 5.26-5.22 (1H, m, CHαtyr), 5.15 (1H, dd, J 4.8, 7.4 Hz, CHαthiapro), 4.84 (1H, d, J 9.3 Hz, NCH _AH_BS), 4.50 (1H, d, J 9.3 Hz, NCH_A H _BS), 3.38 (1H. dd J 7.5, 11.7 Hz, CHCH _AH_BS) 3.25 (1H, dd, J 5.4, 14.3 Hz, CH _AH_BAr), 3.06 (1H, dd, J 4.7, 11.7 Hz, CHCH_A H _BS), and 1.94 (3H, s, NCOCH₃); m/z (ESI, 60V), 513 (M ⁺+1) and the title compound, diastereoisomer B δH (DMSO-d₆, 390K) 9.49 (1H, br s, CSHN) 7.51-7.39 (3H, m, ArH), 7.20 (2H, d, J 8.6 Hz, ArH), 6.95 (2H, d, J 8.6 Hz, ArH), 5.30-5.25 (1H, m, CHαtyr), 5.25 (2H, s, OCH₂Ar), 5.16 (1H, dd, J 4.3, 7.5 Hz, CHαthiopro), 4.81 (1H, d, J 9.4 Hz, NCH _AH_BS), 4.46 (1H, d, J 9.4 Hz, NCH_A H _BS), 3.45 (1H, dd, J 7.5, 11.7 Hz, CHCH _AH_BS), 3.26 (1H, dd, J 5.5, 14.3 Hz, CH _AH_BAr), 3.20-3.10 (2H, m, CH_A H _BAr+CHCH_A H _BS) and 1.93 (3H, s, NCOCH₃); m/z (ESI, 60V) 513 (M ⁺+1).
The following assays can be used to demonstrate the potency and selectivity of the compounds according to the invention. In each of these assays an IC[0125] ₅₀value was determined for each test compound and represents the concentration of compound necessary to achieve 50% inhibition of cell adhesion where 100%=adhesion assessed in the absence of the test compound and 0%=absorbance in wells that did not receive cells.
α[0126] ₄β₁Integrin-Dependent Jurkat Cell Adhesion to VCAM-Ig
96 well NUNC plates were coated with F(ab)[0127] ₂fragment goat anti-human IgG Fcγ-specific antibody [Jackson Immuno Research 109-006-098: 100 μl at 2 μg/ml in 0.1M NaHCO₃, pH 8.4], overnight at 4°. The plates were washed (3×) in phosphate-buffered saline (PBS) and then blocked for 1 h in PBS/1% BSA at room temperature on a rocking platform. After washing (3× in PBS) 9 ng/ml of purified 2d VCAM-Ig diluted in PBS/1% BSA was added and the plates left for 60 minutes at room temperature on a rocking platform. The plates were washed (3× in PBS) and the assay then performed at 37° for 30 min in a total volume of 200 μl containing 2.5×10⁵Jurkat cells in the presence or absence of titrated test compounds.
Each plate was washed (2×) with medium and the adherent cells were fixed with 100 μl methanol for 10 minutes followed by another wash. 100 μl 0.25% Rose Bengal (Sigma R4507) in PBS was added for 5 minutes at room temperature and the plates washed (3×) in PBS. 100 μl 50% (v/v) ethanol in PBS was added and the plates left for 60 min after which the absorbance (570 nm) was measured. [0128]
α[0129] ₄β₇Integrin-Dependent JY Cell Adhesion to MAdCAM-Ig
This assay was performed in the same manner as the α[0130] ₄β₁assay except that MAdCAM-Ig (150 ng/ml) was used in place of 2d VCAM-Ig and a sub-line of the β-lympho blastoid cell-line JY was used in place of Jurkat cells. The IC₅₀value for each test compound was determined as described in the α₄β₁integrin assay.
α[0131] ₅β₁Integrin-Dependent K562 Cell Adhesion to Fibronectin
96 well tissue culture plates were coated with human plasma fibronectin (Sigma F0895) at 5 μg/ml in phosphate-buffered saline (PBS) for 2 hr at 37° C. The plates were washed (3× in PBS) and then blocked for 1 h in 100 μl PBS/1% BSA at room temperature on a rocking platform. The blocked plates were washed (3× in PBS) and the assay then performed at 37° C. in a total volume of 200 μl containing 2.5×10[0132] ⁵K562 cells, phorbol-12-myristate-13-acetate at 10 ng/ml, and in the presence or absence of titrated test compounds. Incubation time was 30 minutes. Each plate was fixed and stained as described in the α₄β₁assay above.
α[0133] _mβ₂-Dependent Human Polymorphonuclear Neutrophils Adhesion to Plastic
96 well tissue culture plates were coated with RPMI 1640/10% FCS for 2 h at 37° C. 2×10[0134] ⁵freshly isolated human venous polymorphonuclear neutrophils (PMN) were added to the wells in a total volume of 200 μl in the presence of 10 ng/ml phorbol-12-myristate-13-acetate, and in the presence or absence of test compounds, and incubated for 20 min at 37° C. followed by 30 min at room temperature. The plates were washed in medium and 100 μl 0.1% (w/v) HMB (hexadecyl trimethyl ammonium bromide, Sigma H5882) in 0.05M potassium phosphate buffer, pH 6.0 added to each well. The plates were then left on a rocker at room temperature for 60 min. Endogenous peroxidase activity was then assessed using tetramethyl benzidine (TMB) as follows: PMN lysate samples mixed with 0.22% H₂O₂(Sigma) and 501 g/ml TMB (Boehringer Mannheim) in 0.1M sodium acetate/citrate buffer, pH 6.0 and absorbance measured at 630 nm.
αIIb/β[0135] ₃-Dependent Human Platelet Aggregation
Human platelet aggregation was assessed using impedance aggregation on the Chronolog Whole Blood Lumiaggregometer. Human platelet-rich plasma (PRP) was obtained by spinning fresh human venous blood anticoagulated with 0.38% (v/v) tri-sodium citrate at 220×g for 10 min and diluted to a cell density of 6×10[0136] ⁸/ml in autologous plasma. Cuvettes contained equal volumes of PRP and filtered Tyrode's buffer (g/liter: NaCl 8.0; MgCl₂.H₂O 0.427; CaCl₂0.2; KCl 0.2; D-glucose 1.0; NaHCO₃1.0; NaHPO₄.2H₂O 0.065). Aggregation was monitored following addition of 2.5 μM ADP (Sigma) in the presence or absence of inhibitors.
In the above assays the compounds of the invention generally have IC[0137] ₅₀values in the α₄β₁and α₄β₇assays of 1 μM and below. In the other assays featuring a integrins of other subgroups the same compounds had IC₅₀values of 50 μM and above thus demonstrating the potency and selectivity of their action against α₄integrins.

Claims

1. A compound of formula (1):

wherein

R¹is a hydrogen atom or an optionally substituted cycloaliphatic, polycycloaliphatic, heterocycloaliphatic, polyheterocycloaliphatic, aromatic or heteroaromatic group;

Alk₁is an optionally substituted aliphatic or heteroaliphatic chain;

L¹is a linker atom or group;

r and s is each zero or an integer 1;

Alk²is a straight or branched alkylene chain;

m; is zero or an integer 1;

R⁴is a hydrogen atom or a methyl group;

R⁵is a hydrogen atom or a straight or branched alkyl group;

R⁶is a group —(CH₂)_tR⁷in which t is zero or the integer 1 and R⁷is an optionally substituted polycycloaliphatic, heterocycloaliphatic, polyhetero-cycloaliphatic, aromatic or heteroaromatic group;

R is a carboxylic acid (—CO₂H) or a derivative thereof;

and the salts, solvates and hydrates thereof.

2. A compound according to claim 1 wherein R is a —CO₂H group.

3. A compound according to claim 1 wherein Alk is a —CH₂— chain and m is an integer 1.

4. A compound according to claim 1 wherein R⁴and R⁵is each a hydrogen atom.

5. A compound according to claim 1 wherein -Alk¹L¹- is a —CH₂O— or —CON(R⁸)— group where R⁸is a hydrogen atom or a straight or branched alkyl group.

6. A compound according to claim 1 wherein R¹is an optionally substituted aromatic or heteroaromatic group.

7. A compound according to claim 6 wherein R¹is an optionally substituted phenyl, pyridyl or pyrimidinyl group.

8. A compound according to claim 1 wherein R⁶is an optionally substituted heterocycloaliphatic, romatic or heteroaromatic group.

9. A compound according to claims 8 wherein R⁶is an optionally substituted pyrrolidinyl, thiazolidinyl, phenyl or pyridyl group.

10. A pharmaceutical composition comprising a compound of formula (1):

wherein

Alk¹is an optionally substituted aliphatic or heteroaliphatic chain;

L¹is a linker atom or group,

r and s is each zero or an integer 1;

Alk²is a straight or branched alkylene chain;

m is zero or an integer 1;

R⁴is a hydrogen atom or a methyl group;

R⁵is a hydrogen atom or a straight or branched alkyl group;

R is a carboxylic acid (—CO₂H) or a derivative thereof;

and the salts, solvates and hydrates thereof; together with one or more pharmaceutically acceptable carriers, excipients or diluents.