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UA117935C2 - METHOD OF OBTAINING L-AMINO ACID - Google Patents

METHOD OF OBTAINING L-AMINO ACID

Info

Publication number
UA117935C2
UA117935C2 UAA201603717A UAA201603717A UA117935C2 UA 117935 C2 UA117935 C2 UA 117935C2 UA A201603717 A UAA201603717 A UA A201603717A UA A201603717 A UAA201603717 A UA A201603717A UA 117935 C2 UA117935 C2 UA 117935C2
Authority
UA
Ukraine
Prior art keywords
target
gene
amino acid
obtaining
corinform
Prior art date
Application number
UAA201603717A
Other languages
Ukrainian (uk)
Inventor
Чун Ок Моон
Сань Чо Лім
То Хьюн Квон
Кван Хо Лі
Хьюн Вон Пе
Original Assignee
Чх-Ч Чхейлчетан Корп.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Чх-Ч Чхейлчетан Корп. filed Critical Чх-Ч Чхейлчетан Корп.
Priority claimed from PCT/KR2014/009468 external-priority patent/WO2015053552A1/en
Publication of UA117935C2 publication Critical patent/UA117935C2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y403/00—Carbon-nitrogen lyases (4.3)
    • C12Y403/03—Amine-lyases (4.3.3)
    • C12Y403/03007—4-Hydroxy-tetrahydrodipicolinate synthase (4.3.3.7)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Винахід стосується способу одержання L-амінокислот, що включає культивування рекомбінантного корінеформного мікроорганізму, що продукує L-амінокислоти, де рекомбінантний корінеформний мікроорганізм трансформують шляхом введення промотору нижче стоп-кодону гена-мішені в хромосомі, де позиція нижче стоп-кодону знаходиться між стоп-кодоном і вище транскрипційного термінатора гена-мішені або нижче термінатора транскрипції гена-мішені, і де ген-мішень є геном, розташованим у точці розгалуження шляху біосинтезу L-амінокислот.The invention relates to a method for producing L-amino acids, comprising cultivating a recombinant corinform microorganism producing L-amino acids, where the recombinant corinform microorganism is transformed by introducing a promoter below the stop codon of the gene-target, where the target is located and above the transcriptional terminator of the target gene or below the transcriptional terminator of the target gene, and where the target gene is a gene located at the point of branching of the pathway of L-amino acid biosynthesis.

UAA201603717A 2013-10-11 2014-08-10 METHOD OF OBTAINING L-AMINO ACID UA117935C2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20130121090 2013-10-11
KR1020140091307A KR101518860B1 (en) 2013-10-11 2014-07-18 Method for producing L-amino acid
PCT/KR2014/009468 WO2015053552A1 (en) 2013-10-11 2014-10-08 Method of producing l-amino acids

Publications (1)

Publication Number Publication Date
UA117935C2 true UA117935C2 (en) 2018-10-25

Family

ID=53035683

Family Applications (1)

Application Number Title Priority Date Filing Date
UAA201603717A UA117935C2 (en) 2013-10-11 2014-08-10 METHOD OF OBTAINING L-AMINO ACID

Country Status (16)

Country Link
JP (3) JP6291042B2 (en)
KR (1) KR101518860B1 (en)
CN (1) CN104561163B (en)
AR (1) AR097977A1 (en)
AU (2) AU2014332732B2 (en)
BR (1) BR112016008029A2 (en)
CA (1) CA2925659C (en)
DK (1) DK2860256T3 (en)
ES (1) ES2626048T3 (en)
GE (2) GEP20196943B (en)
JO (1) JO3255B1 (en)
MY (1) MY172463A (en)
PH (1) PH12016500640A1 (en)
PL (1) PL2860256T3 (en)
RU (2) RU2651461C2 (en)
UA (1) UA117935C2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101518860B1 (en) * 2013-10-11 2015-05-12 씨제이제일제당 (주) Method for producing L-amino acid
CN105734004B (en) * 2016-03-02 2020-06-19 廊坊梅花生物技术开发有限公司 Recombinant strain and preparation method and application thereof
KR101925930B1 (en) * 2017-07-24 2019-02-26 씨제이제일제당 주식회사 Microorganisms producing L-lysine and process for producing L-lysine using the same
WO2019172702A1 (en) * 2018-03-09 2019-09-12 씨제이제일제당 (주) Novel promoter and l-amino acid production method using same
KR102221040B1 (en) * 2019-05-09 2021-03-03 씨제이제일제당 주식회사 Microorganism producing L-amino acid and method of producing Method of L-amino acid using thereof
KR102134418B1 (en) * 2019-06-17 2020-07-16 씨제이제일제당 주식회사 A microorganism producing L-tyrosine and a method for producing L-tyrosine using thereof
KR102207867B1 (en) * 2020-01-21 2021-01-26 씨제이제일제당 주식회사 Method for producing L-amino acids using microorganisms containing NADP dependent glyceraldehyde-3-phosphate dehydrogenase
KR102527895B1 (en) * 2021-01-11 2023-04-28 씨제이제일제당 (주) GlxR protein variant or threonine production method using the same
CN115678933A (en) * 2022-09-26 2023-02-03 廊坊梅花生物技术开发有限公司 A method for improving the conversion rate of lysine fermentation

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6649379B1 (en) * 1987-06-12 2003-11-18 Massachusetts Institute Of Technology Method and deregulated enzyme for threonine production
JP3036912B2 (en) * 1991-09-02 2000-04-24 協和醗酵工業株式会社 Gene expression regulatory DNA
JP3783065B2 (en) * 1994-03-04 2006-06-07 味の素株式会社 Method for producing L-lysine
ES2373863T3 (en) * 1995-06-07 2012-02-09 Ajinomoto Co., Inc. L-LISINE PRODUCTION PROCEDURE.
EP1029919B1 (en) * 1999-02-20 2004-03-31 Degussa AG Process for the fermentative production of L-amino acids using coryneform bacteria
KR20020073153A (en) * 1999-12-24 2002-09-19 아지노모토 가부시키가이샤 Process for producing L-amino acid and novel gene
WO2002040679A2 (en) * 2000-11-15 2002-05-23 Archer-Daniels-Midland Company Corynebacterium glutamicum promoters
KR100397423B1 (en) * 2001-02-13 2003-09-13 씨제이 주식회사 Process for producing L-threonine
DE10224088A1 (en) * 2002-05-31 2003-12-11 Degussa Process for the production of L-amino acids using coryneform bacteria which contain an attenuated mez gene
JP2004261150A (en) * 2003-03-04 2004-09-24 Ajinomoto Co Inc Method for producing objective material
JP2005006564A (en) 2003-06-19 2005-01-13 Japan Tobacco Inc Nitrate ion-inducing promoter
JP2010226956A (en) * 2007-07-23 2010-10-14 Ajinomoto Co Inc Method for producing l-lysine
KR101335853B1 (en) 2011-12-01 2013-12-02 씨제이제일제당 (주) A microorganism having L-amino acids and riboflavin productivity and a method of producing L-amino acids and riboflavin using the same
KR101518860B1 (en) * 2013-10-11 2015-05-12 씨제이제일제당 (주) Method for producing L-amino acid

Also Published As

Publication number Publication date
JP6291042B2 (en) 2018-03-14
KR20150042692A (en) 2015-04-21
CN104561163A (en) 2015-04-29
DK2860256T3 (en) 2017-06-06
PH12016500640B1 (en) 2016-05-30
AR097977A1 (en) 2016-04-20
AU2014332732B2 (en) 2017-12-07
AU2014332732A1 (en) 2016-04-21
JO3255B1 (en) 2018-09-16
RU2651461C2 (en) 2018-04-19
PL2860256T3 (en) 2017-08-31
MY172463A (en) 2019-11-26
AU2017272295B2 (en) 2019-05-16
RU2614258C2 (en) 2017-03-24
CA2925659A1 (en) 2015-04-16
JP2020078312A (en) 2020-05-28
PH12016500640A1 (en) 2016-05-30
CN104561163B (en) 2018-11-06
AU2017272295A1 (en) 2018-01-04
GEAP201814125A (en) 2018-10-10
BR112016008029A2 (en) 2017-10-03
JP6824918B2 (en) 2021-02-03
CA2925659C (en) 2018-10-23
ES2626048T3 (en) 2017-07-21
RU2015151124A (en) 2016-06-27
GEP20196943B (en) 2019-02-11
JP2016532439A (en) 2016-10-20
RU2016110804A (en) 2016-07-27
JP2018113966A (en) 2018-07-26
KR101518860B1 (en) 2015-05-12

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