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UA116437C2 - MUTANT TOBACCO PLANTS CONDITIONAL WITH LOW EXPRESSION OF ISOPROPYLMALSINTAZE, METHOD OF ITS RECEIVING AND USE - Google Patents

MUTANT TOBACCO PLANTS CONDITIONAL WITH LOW EXPRESSION OF ISOPROPYLMALSINTAZE, METHOD OF ITS RECEIVING AND USE

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Publication number
UA116437C2
UA116437C2 UAA201403274A UAA201403274A UA116437C2 UA 116437 C2 UA116437 C2 UA 116437C2 UA A201403274 A UAA201403274 A UA A201403274A UA A201403274 A UAA201403274 A UA A201403274A UA 116437 C2 UA116437 C2 UA 116437C2
Authority
UA
Ukraine
Prior art keywords
isopropylmalsintaze
conditional
receiving
tobacco plants
low expression
Prior art date
Application number
UAA201403274A
Other languages
Ukrainian (uk)
Inventor
Ніколя Бакаєр
Грегор Ніколас Біндлер
Мішель Філіпп Блан
Сімон Гепферт
Флоріан Мартін
Original Assignee
Філіп Морріс Продактс С.А
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Філіп Морріс Продактс С.А filed Critical Філіп Морріс Продактс С.А
Publication of UA116437C2 publication Critical patent/UA116437C2/en

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    • CCHEMISTRY; METALLURGY
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18Treatment of tobacco products or tobacco substitutes
    • A24B15/20Biochemical treatment
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    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H13/00Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids
    • C07H13/02Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids
    • C07H13/04Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids having the esterifying carboxyl radicals attached to acyclic carbon atoms
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • C12N9/1029Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
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    • C12YENZYMES
    • C12Y203/00Acyltransferases (2.3)
    • C12Y203/03Acyl groups converted into alkyl on transfer (2.3.3)
    • C12Y203/030132-Isopropylmalate synthase (2.3.3.13)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Manufacture Of Tobacco Products (AREA)

Abstract

Винахід стосується мутантної, що не зустрічається в природі, або трансгенної рослинної клітини, що включає в себе рекомбінантну конструкцію, яка включає полінуклеотид, що кодує ізопропілмалатсинтазу, функціонально зв'язану з трихома-специфічним промотором, або полінуклеотид, який знижує або інгібує експресію ізопропілмалатсинтази, функціонально зв'язаної з трихома-специфічним промотором; рослинного матеріалу, що її містить, а також способу отримання бета-метилвалеріанової кислоти та способу модулювання смаку тютюну за допомогою використання зазначеної мутантної рослини.The invention relates to a mutant non-naturally occurring or transgenic plant cell comprising a recombinant construct that includes a polynucleotide encoding isopropylmalatase synthase, functionally linked to a trichome-specific promoter, or polynucleotide, which is an excipi functionally linked to a trichome-specific promoter; the plant material containing it, as well as a method of producing beta-methyl valeric acid and a method of modulating the taste of tobacco by using the specified mutant plant.

UAA201403274A 2011-09-02 2012-08-31 MUTANT TOBACCO PLANTS CONDITIONAL WITH LOW EXPRESSION OF ISOPROPYLMALSINTAZE, METHOD OF ITS RECEIVING AND USE UA116437C2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11179882A EP2565265A1 (en) 2011-09-02 2011-09-02 Isopropylmalate synthase from Nicotiana tabacum and methods and uses thereof
PCT/EP2012/003662 WO2013029799A1 (en) 2011-09-02 2012-08-31 Isopropylmalate synthase from nicotiana tabacum and methods and uses thereof

Publications (1)

Publication Number Publication Date
UA116437C2 true UA116437C2 (en) 2018-03-26

Family

ID=46832334

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Application Number Title Priority Date Filing Date
UAA201403274A UA116437C2 (en) 2011-09-02 2012-08-31 MUTANT TOBACCO PLANTS CONDITIONAL WITH LOW EXPRESSION OF ISOPROPYLMALSINTAZE, METHOD OF ITS RECEIVING AND USE

Country Status (19)

Country Link
US (1) US10287599B2 (en)
EP (3) EP2565265A1 (en)
JP (2) JP2014531200A (en)
KR (1) KR102012993B1 (en)
CN (1) CN103958673B (en)
AU (1) AU2012301349B2 (en)
BR (1) BR112014004827A8 (en)
ES (1) ES2742271T3 (en)
IL (1) IL231101B (en)
IN (1) IN2014CN01706A (en)
MX (1) MX365497B (en)
MY (1) MY172423A (en)
PH (1) PH12014500455A1 (en)
PL (1) PL2751262T3 (en)
RU (1) RU2644238C2 (en)
SG (1) SG11201400209UA (en)
TR (1) TR201910626T4 (en)
UA (1) UA116437C2 (en)
WO (1) WO2013029799A1 (en)

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CN111818795A (en) 2018-03-28 2020-10-23 菲利普莫里斯生产公司 Modulating reducing sugar content in plants
WO2019185703A1 (en) 2018-03-28 2019-10-03 Philip Morris Products S.A. Modulating amino acid content in a plant
EP3902390A1 (en) 2018-12-30 2021-11-03 Philip Morris Products S.A. Modulation of nitrate levels in plants via mutation of nitrate reductase
CN110610743A (en) * 2019-08-16 2019-12-24 广东省生态环境技术研究所 Method for constructing chromium reductase protein database
KR20220070305A (en) 2019-10-01 2022-05-30 필립모리스 프로덕츠 에스.에이. Control of reducing sugar (INV) content in plants
KR20220071260A (en) 2019-10-01 2022-05-31 필립모리스 프로덕츠 에스.에이. Control of sugar and amino acid (SULTR3) content in plants
AU2021252558A1 (en) * 2020-04-07 2022-11-10 Board Of Trustees Of Michigan State University Improving plant nutritional value and growth through enhancement of essential amino acid levels
KR102527102B1 (en) 2021-03-05 2023-04-28 씨제이제일제당 주식회사 Isopropylmaleate synthase variant and a method of producing L-leucine using the same
US20240368615A1 (en) 2021-09-10 2024-11-07 Philip Morris Products S.A. Modulating alkaloid profiles in nicotiana tabacum
US20250043298A1 (en) 2021-12-20 2025-02-06 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
WO2023117701A1 (en) 2021-12-21 2023-06-29 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants
EP4602060A1 (en) 2022-10-13 2025-08-20 Philip Morris Products S.A. Increasing leaf biomass and nitrogen use efficiency by regulating ntp2
EP4658792A1 (en) 2023-02-02 2025-12-10 Philip Morris Products S.A. Modulation of sugar transporters
WO2024160860A1 (en) 2023-02-02 2024-08-08 Philip Morris Products S.A. Modulation of genes coding for lysine ketoglutarate reductase
WO2025003105A1 (en) 2023-06-29 2025-01-02 Philip Morris Products S.A. Modulation of genes coding for glutamate dehydrogenase
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CN119776365A (en) * 2024-11-15 2025-04-08 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) Tobacco sucrose ester synthesis related gene NtBMVSE and application thereof
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Publication number Publication date
TR201910626T4 (en) 2019-08-21
BR112014004827A8 (en) 2018-03-13
PH12014500455A1 (en) 2014-04-14
PL2751262T3 (en) 2020-01-31
MX365497B (en) 2019-06-05
EP2751262A1 (en) 2014-07-09
MY172423A (en) 2019-11-25
EP2751262B1 (en) 2019-06-12
JP2014531200A (en) 2014-11-27
JP2018186820A (en) 2018-11-29
WO2013029799A1 (en) 2013-03-07
RU2014112234A (en) 2015-10-10
ES2742271T3 (en) 2020-02-13
BR112014004827A2 (en) 2017-04-04
RU2644238C2 (en) 2018-02-08
AU2012301349A1 (en) 2014-03-20
AU2012301349B2 (en) 2015-09-17
IN2014CN01706A (en) 2015-05-29
KR20140067082A (en) 2014-06-03
IL231101A0 (en) 2014-04-30
CN103958673A (en) 2014-07-30
US10287599B2 (en) 2019-05-14
IL231101B (en) 2018-10-31
KR102012993B1 (en) 2019-08-21
SG11201400209UA (en) 2014-03-28
MX2014002497A (en) 2014-12-04
JP7000265B2 (en) 2022-02-04
EP3578645A1 (en) 2019-12-11
EP2565265A1 (en) 2013-03-06
CN103958673B (en) 2021-10-26
US20140352706A1 (en) 2014-12-04

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