TWM615103U - Nucleic acid extracting device - Google Patents

Abstract

This creation is a nucleic acid extraction device, including: a base, a rotating unit and a microcentrifuge tube; the base has a pair of positions in the middle, and the base is additionally provided with a movable first moving part and a first moving part A magnet end is provided. When the first moving part moves, the magnet end moves in the alignment area; the rotating unit includes a bearing plate and two brackets erected on the base. The middle of the bearing plate has a bearing hole, and the two ends of the bearing plate It is pivotally connected to the support and can be rotated; the microcentrifuge tube is inserted into the bearing hole, the bottom of the microcentrifuge tube is located in the alignment area, and the magnet end can be close to the outer wall of the bottom of the microcentrifuge tube; Professionals can perform nucleic acid extraction and detection operations without any equipment in a general indoor environment.

Description

Nucleic acid extraction device

This creation is the technical field of a nucleic acid extraction device, in particular, it refers to a nucleic acid detection method that uses this device with a simple operation method, and a non-professional person can complete the nucleic acid detection operation.

As the technology of biotechnology matures, more and more bioassays use nucleic acids from samples for experiments and inspections. The inspection process includes two parts: "nucleic acid extraction" and "nucleic acid amplification reaction". Common nucleic acid extraction methods include: column extraction method, reagent extraction method and magnetic bead extraction method. Among them, the magnetic bead extraction method is relatively simple and is the current mainstream method. After that, the purified nucleic acid is added to the enzyme to produce a reaction, and a small amount of nucleic acid undergoes a biochemical reaction to make a measurable number of nucleic acid copies, which is called a "nucleic acid amplification reaction."

As shown in Figure 1, it is a schematic diagram of the current magnetic bead extraction method. This method uses the surface of the magnetic beads to construct functional groups to adsorb nucleic acids, and then separate and extract the nucleic acids. Lysis step (A): Add the lysis buffer 11 and the biological sample 12 into the centrifuge microtube 10, and mix the lysis buffer 11 with the biological sample 12 to destroy the cell or virus sheath to release nucleic acid. Binding step (B): adding a binding buffer 13 containing magnetic beads to promote the adsorption of nucleic acids on the surface of the magnetic beads. Washing step (C): Use wash buffer 14 to wash magnetic beads to remove non-specific adsorbents, use magnet 15 to attract magnetic beads to retain nucleic acids and remove lipids, protein impurities and cell debris. Professionals must be employed in the process A professional pipette (Pipette) 16 is used to remove the liquid, but the removal of magnetic beads must be avoided. This cleaning operation must be performed three times. Separation step (D): Use a low-salinity elution buffer 17 to remove the nucleic acid from the magnetic beads. In the collection step (E), the magnetic beads are attracted by the magnet 15 and the liquid is collected by the professional pipette 16 to obtain purified nucleic acid in the liquid phase.

However, the above magnetic bead extraction method must be equipped with an electric mixing device in the laboratory to perform inspections, and the operation must also be performed by experienced operators. The waste liquid or nucleic acid can be extracted when using a professional pipette 16 Liquid, cannot be operated by ordinary personnel. Therefore, in the current new coronary pneumonia that requires a large number of and rapid nucleic acid detection operations, a convenient device and an easy-to-detect method are sought, which is the problem that the author wants to solve.

The main purpose of this creation is to provide a nucleic acid extraction device. With this device and a set of nucleic acid detection methods, non-professionals can perform nucleic acid extraction and detection operations in a general indoor environment, with high accuracy, convenient and easy operation. This can reduce the workload of professional inspectors and meet the needs of a large number of inspection operations in a short period of time.

In order to achieve the above-mentioned purpose, this creation is a nucleic acid extraction device, including: a base, a rotating unit and a microcentrifuge tube. There is an alignment area in the middle of the base, and the base is additionally provided with a movable first moving piece, the first moving piece is provided with a magnet end, and the magnet end will move in the alignment area when the first moving piece moves; the rotating unit It includes a bearing plate and two brackets erected on the base. The middle of the bearing plate has a bearing hole. The two ends of the bearing plate are pivotally connected to the bracket and can be rotated; The bottom of the centrifuge tube can be located in the alignment area, and the magnet end can be close to the outer wall of the bottom of the microcentrifuge tube.

As one of the preferred embodiments, the base further includes a movable second moving part, the second moving part is separated from the first moving part by 180 degrees, the second moving part has a positioning notch, and the second moving part is moved The positioning notch can be moved to the central position of the alignment area, and the positioning notch can be used for the bottom end of the microcentrifuge tube to be located therein, so as to facilitate the contact between the magnet end and the outer wall of the microcentrifuge tube.

As one of the preferred embodiments, the supporting plate is provided with a plate mark, and the microcentrifuge tube is also provided with a tube mark. When the microcentrifuge tube is placed in the supporting well, the plate mark corresponds to the tube mark.

As one of the preferred preferred embodiments, it further includes an adsorption material. The adsorption material is cotton swabs, sponges, fibers and materials that can absorb liquid. The adsorption material is inserted into the microcentrifuge tube to absorb the pyrolysis used in the extraction process. Buffer, binding buffer, washing buffer.

As one of the preferred preferred embodiments, the shape of the adsorption material corresponds to the shape of the microcentrifuge tube, but the bottom has a slope. When the adsorption material is inserted into the microcentrifuge tube, the slope will not contact the inner wall of the tube. The purpose is to make the slope Will not touch the magnetic beads.

As one of the preferred embodiments, the microcentrifuge tube has a tube mark, and the adsorption material has a marking image. When the adsorption material is inserted into the microcentrifuge tube, the tube mark and the marking image are aligned with each other.

Compared with the prior art, the nucleic acid extraction device and the nucleic acid detection method used in this invention have the following specific effects:

1. It can be operated without expensive electric mixing equipment, which is convenient for the operator to perform detection in a general indoor environment;

2. This creation uses absorbent material to absorb the liquid in the microcentrifuge tube. This method does not require professionals to use a pipette to extract the liquid, thereby simplifying the difficulty of separating the liquid and facilitating the detection operation.

3. The adsorption material of this creation has an inclined surface at the bottom. When the adsorption material is inserted into the microcentrifuge tube, the inclined surface is in contact with the inner wall of the tube at a small distance, so that the inclined surface will not contact the magnetic beads and avoid interference with the magnetic beads Nucleic acid, which helps non-professionals to perform solid-liquid separation operations.

4. At the end of this creation, after the nucleic acid is attached to the magnetic beads and dried, the enzyme and nucleic acid primers are added to allow the nucleic acid to undergo amplification reaction. This is different from the conventional method and also makes the operation easier.

5. Since this creation does not need to be performed in a laboratory or by professionals, it can be performed indoors and by ordinary personnel following the above methods, which can greatly increase the amount of inspections and meet the needs of a large number of tests in a short time .

The technical solution of this creation will be clearly and completely described below in conjunction with specific embodiments and drawings. All technical and scientific terms used in this article have the same meanings as those commonly understood by those skilled in the creative technical field. The terminology used herein is only for the purpose of describing specific embodiments, and is not intended to limit the creation. The term "and/or" as used herein includes any and all combinations of one or more related listed items.

In addition, the following terms used in this creation specification and each claim have the definitions given below. The singular term "a" used in the specification and each claim is intended to cover one and more than one of the contained items, such as at least one, at least two, or at least three, rather than implying only a single contained item . In addition, the open connectives such as "include" and "have" used in each claim in this case mean that the combination of elements or components described in the claim does not exclude other elements or components that are not specified in the claim. "Basically composed of" means that the described combination of ingredients does not exclude the inclusion of other undocumented elements or ingredients that will not substantially affect the composition and nature of the requested creation.

The term "nucleic acid" used in this specification is intended to cover polymers composed of nucleotide bases, including but not limited to deoxyribonucleic acid (DNA), ribonucleic acid (RNA), microRNA (miRNA) and The combination of them, where the DNA can be genomic DNA, mitochondrial DNA, chromosome fragments, DNA fragments, plastid DNA, etc. The term "nucleic acid extraction" used in the description of this case includes but is not limited to the purification process of lysing cells or virus sheaths, then capturing nucleic acids, and then isolating nucleic acids from biological samples. The term "biological sample" used in the description of this case is intended to cover samples collected by organisms for extraction operations, including but not limited to plant tissues, animal tissues or human tissues; cell populations; eukaryotic or prokaryotic cells; and viruses . In a specific example, the so-called "biological sample" can be from human or animal blood, plasma, serum, urine, saliva, sputum, semen, tears, ascites, amniotic fluid, or cerebrospinal fluid and other body fluids.

The various buffer terms used in this creation specification are buffers prepared by laboratories or commercially available. For example, lysis buffer is a buffer solution used to destroy open cells. The binding buffer contains magnetic beads. The main component of this liquid is isopropanol, which helps to promote the adsorption of nucleic acids to the magnetic beads and/or reduce the non-specific adsorption of proteins to the magnetic beads. The wash buffer contains high-concentration ethanol to wash impurities and purify nucleic acids.

The "magnetic beads" used in this creation manual are nanomaterial-grade magnetic beads, which refer to superparamagnetic silica beads with a diameter between 10 nm and 1,000 nm, preferably 300 nm in diameter. Within the range of 1,000 nanometers, it has a superparamagnetic core made of ferromagnetic materials and an outer layer of silica, optionally with additional surface modification, so that nucleic acids (such as DNA) can be cracked and extracted. In the environment established by the buffer solution, it is adsorbed on the surface of the magnetic beads, and detached from the magnetic beads in a low-salinity environment. In a specific example, the core of the nano-grade magnetic beads is made of Fe₃O₄, so the magnetic beads have permanent magnetism. When an external magnetic field is applied, the nano-scale magnetic beads will move toward the orientation and be concentrated and fixed under the drive of the magnetic field. When the external magnetic field is removed, the magnetic moment of the magnetic beads disappears, which is called superparamagnetism.

The term "nucleic acid reaction" used in this creation specification is intended to cover all chemical, physical and enzymatic reactions of nucleic acids, especially nucleic acid amplification reactions. "Nucleic acid amplification reaction" means that a small amount of nucleic acid undergoes a biochemical reaction to make a measurable amount of nucleic acid copy, which includes but is not limited to polymerase chain reaction (PCR)-based amplification reactions, such as PCR, quantitative PCR ( qPCR), nested PCR, reverse transcription polymerase chain reaction (RT-PCR), LAMP PCR (Loop-Mediated Isothermal Amplification), linear amplification, etc.; non-PCR-based amplification reactions, such as multiplex Displacement amplification reaction (MDA), transcription-mediated amplification reaction (TMA), nucleic acid sequence-dependent amplification reaction (NASBA), strand displacement amplification reaction (SDA), real-time SDA, rolling circle amplification reaction ( RCA), loop-to-loop amplification reaction (C2CA), etc.; and their combination. In the case that the biological sample contains only a very small amount of target nucleic acid, the nucleic acid amplification reaction is a necessary means to complete the detection.

Next, a brief description of the nucleic acid extraction device of the invention is given, as shown in Figure 2. The inventive nucleic acid extraction device includes: a base 20, a rotating unit 30, and a microcentrifuge tube 40. The microcentrifuge tube 40 is used for the injection of nucleic acid-containing biological products or various buffers. The rotating unit 30 is installed on the base 20 and is responsible for driving the microcentrifuge tube 40 to rotate to achieve the desired mixing effect. In addition, other structures are provided on the base 20 to facilitate nucleic acid extraction.

The following is a detailed description of the structure of each component of this creation:

Please refer to FIG. 3 together. The base 20 has a pair of position areas 21 in the middle, and a first moving part 22 and a second moving part 23 that are movable. The first moving member 22 is in the shape of a long strip and has a magnetic body 221 at one end. The magnetic body 221 is composed of a magnet. The first moving member 22 partially extends outside the base 20 and moves to make the magnetic body 221 move synchronously in the alignment area 21, and the magnetic body 221 can move to the center of the alignment area 21. The position of the second moving part 23 is 180 degrees apart from the first moving part 22. The second moving part 23 is in the shape of a long strip and has a positioning notch 231 at one end. The second moving part 23 partially extends outside the base 20 and can move the positioning notch 231 synchronously in the alignment area 21. The positioning notch 231 can be moved to the center of the alignment area 21. In this embodiment, when the positioning notch 231 and the magnetic body 221 are both located in the center of the alignment area 21, the bottom end of the microcentrifuge tube 40 can be positioned in the positioning notch 231, and the magnetic body 221 is in contact with the outer wall of the microcentrifuge tube 40 . The magnetic body 221 adsorbs the magnetic beads in the tube on the outer wall of the tube.

The rotating unit 30 includes a supporting plate 31 and two brackets 32 erected on the base 20. The middle of the bearing plate 31 has a bearing hole 33. The two ends of the bearing plate 31 are pivotally connected to the bracket 32 and can be rotated. In this embodiment, a handle 34 can be further installed on the side of the bearing plate 31. The bearing plate 31 is driven to rotate synchronously. A plate mark 311 is provided on the supporting plate 31 near the supporting hole 33, and the plate mark 311 is used for aligning the microcentrifuge tube 40. The board mark 311 can be a color mark, an arrow symbol, or various patterns for identification.

Eppendorf 40 is a test tube commonly used in laboratories, and has a lid 41 for capping. In this embodiment, in order to facilitate the removal of various buffers added during the experiment, a specially designed absorbent material 45 is used. The absorbent material 45 can be cotton swabs, sponges, fibers, and materials that can absorb liquid. As shown in Figure 4. The shape of the adsorption material 45 corresponds to the shape of the microcentrifuge tube 40. In other words, the upper half of the adsorption material 45 is cylindrical, the size corresponds to the inner diameter of the microcentrifuge tube 40, and the lower half is tapered and has an inclined surface 451. There is a mark 452 on the top of the adsorption material 45. The microcentrifuge tube 40 has a tube mark 42. In this embodiment, the tube mark 42 is provided on the lid 41, but it is not limited to this, and can also be provided on the outer wall of the microcentrifuge tube 40. In this embodiment, the marking image 452 and the tube marking 42 may be colored markings, but are not limited to this, and may also be other recognizable patterns. When the adsorbing material 45 is inserted into the microcentrifuge tube 40, the marking image 452 must be aligned with the tube mark 42 so that the inclined surface 451 and the inner wall of the predetermined position at the bottom of the microcentrifuge tube 40 have a distance. This predetermined position is where the magnetic beads are expected to be adsorbed. In addition, when the microcentrifuge tube 40 is placed in the receiving hole 33 of the supporting plate 31, the tube mark 42 must also be aligned with the plate mark 311.

As shown in Figure 5, the flow chart of this author's nucleic acid detection method. The nucleic acid detection method of the present invention includes the following steps: Step 501: adding a biological sample containing nucleic acid to a microcentrifuge tube containing a lysis buffer and mixing to lyse the nucleic acid; Step 502: Add binding buffer containing magnetic beads to the microcentrifuge tube, use magnetic beads to bind nucleic acid, and then adsorb the magnetic beads in the tube with a magnetic body on the outer wall of the tube, and then adsorb and remove the binding buffer after the reaction by the adsorption material; 503: Add the cleaning buffer and mix in the microcentrifuge tube, and then use the magnetic body to adsorb the magnetic beads in the tube from the outer wall of the tube, and then adsorb and remove the cleaning buffer after the reaction by another adsorption material; Step 504: Take out the test tube at a predetermined temperature After drying, add a buffer solution containing enzymes and nucleic acid primers to a microcentrifuge tube for nucleic acid amplification reaction.

Step 503 can be performed several times as required. In this embodiment, two cleaning steps are performed.

Next, this creation will use a specific example, based on the above steps, in conjunction with the creation of the nucleic acid extraction device, to give a clearer description of the nucleic acid detection method. In the initial state, adjust the positions of the first moving part 22 and the second moving part 23 on the base 20 so that the magnetic body 221 and the positioning notch 231 are separated from the central position of the alignment area 21 (as shown in FIG. 2) to avoid Interference with the rotation of the microcentrifuge tube 40.

As shown in step 501 and as shown in Figure 6A, Figure 6B, and Figure 6C, perform the [Sample Preparation] operation: add 50 microliters (μl) of biological samples containing viral nucleic acids (such as liquid) to 400μl-lysis buffer In the microcentrifuge tube 40 (as shown in FIG. 6A), in order to facilitate the reaction, 150 μl of phosphate buffered saline (PBS) and 50 μl of sample solution can be added. The microcentrifuge tube 40 is inserted into the receiving hole 33 of the supporting plate 31, and the supporting plate 31 is rotated for 5 minutes (as shown in FIG. 6B), thereby lysing the nucleic acid (as shown in FIG. 6C).

As shown in step 502 and Figure 7A, Figure 7B, Figure 7C and Figure 7D, perform the [nucleic acid binding] task: add 400μl of binding buffer containing magnetic beads to the microcentrifuge tube 40 (Figure 7A). The binding buffer is mainly The ingredient is isopropanol. Rotate the carrier plate 31 for 5 minutes, so that the nucleic acid is adsorbed by the magnetic beads and attached to the periphery (as shown in FIG. 7B). The second moving member 23 is pushed to move, so that the positioning notch 231 is located directly below the bearing hole 33. Push the first moving part 22 Move so that the magnetic body 221 is located on the outer wall of the bottom of the microcentrifuge tube 40, wait for 3 minutes, and then the magnetic beads in the microcentrifuge tube 40 are adsorbed by the magnetic body 221. At this time, the magnetic beads attached to the nucleic acid will gather on the inner wall of the microcentrifuge tube 40 (as shown in FIG. 7C). Use the adsorbing material 45 to align the tube mark 42 with the mark figure 452 and insert it into the microcentrifuge tube 40, and use the capillary phenomenon to adsorb and remove the binding buffer after the reaction. At this time, the inclined surface 451 is a short distance away from the magnetic beads gathering place (Figure 7D) , To avoid the adsorption material 45 interference or contact with the magnetic beads, affecting the attached nucleic acid. After that, the adsorption material 45 is taken out and discarded. The second moving part 23 and the first moving part 22 are moved again, so that the positioning notch 231 and the magnetic body 221 exit the center position of the alignment area 21.

As in step 503 and FIG. 8A, FIG. 8B, FIG. 8C, and FIG. 8D, perform the [washing step] operation: add 400 μl-washing buffer into the microcentrifuge tube 40 (as shown in FIG. 8A). Rotate the carrier plate 31 for 3 minutes (Figure 8B). The impurities are dissolved in the washing buffer 14. The second moving member 23 is pushed to move, so that the positioning notch 231 is located directly below the bearing hole 33. The first moving member 22 is pushed to move, so that the magnetic body 221 is located on the outer wall of the bottom of the microcentrifuge tube 40, and after waiting for 1 minute, the magnetic body 221 is used to adsorb the magnetic beads in the microcentrifuge tube 40 (as shown in FIG. 8C). The magnetic beads to which the nucleic acid is attached will gather on the inner wall of the microcentrifuge tube 40. The adsorbing material 45 is inserted into the microcentrifuge tube 40 with the mark image 452 aligned with the tube mark 42, and the cleaning buffer after the reaction is adsorbed and removed by the capillary phenomenon (as shown in FIG. 8D). Take out the adsorption material 45 and discard it. After that, a second wash is performed, and 600 μl-washing buffer is added to the microcentrifuge tube 40 (as shown in FIG. 8A). Rotate the carrier plate 31 for 3 minutes (Figure 8B). Dissolve impurities in the wash buffer. The second moving member 23 is pushed to move, so that the positioning notch 231 is located directly below the bearing hole 33. The first moving member 22 is pushed to move, so that the magnetic body 221 is located on the outer wall of the bottom of the microcentrifuge tube 40, and after waiting for 1 minute, the magnetic body 221 is used to adsorb the magnetic beads in the microcentrifuge tube 40. The magnetic beads to which the nucleic acid is attached will gather on the inner wall of the microcentrifuge tube 40 (as shown in FIG. 8C). The adsorbing material 45 is inserted into the microcentrifuge tube 40 with the mark 452 aligned with the tube mark 42, and the post-reaction cleaning buffer 14 is adsorbed and removed by using the capillary phenomenon (as shown in Fig. D). Take out the adsorption material 45 and discard it. In this embodiment, only two cleaning operations are required.

As shown in step 504 and Figures 9A, 9B and 9C, proceed to [nucleic acid amplification reaction]: as shown in Figure 9A and Figure 9B, take out the microcentrifuge tube 40 and place it in a dry bath heater 50 at 60-70°C. Let dry for at least 3 minutes. The dry bath heater 50 uses heating powder to generate and maintain a predetermined temperature. Then add a buffer solution containing enzymes and nucleic acid primers to the microcentrifuge tube 40, close the lid 41, so that it is submerged in the dry bath heater 50 (as shown in Figure 9C), and cover with an upper lid 51 to seal it, maintaining it at 60 Amplify the nucleic acid attached to the magnetic beads under the condition of -70℃. After standing for a few 10 minutes, take out the microcentrifuge tube 40 according to the color change produced by the nucleic acid amplification amount to determine whether it has a preset The nucleic acid virus.

Based on the above, the nucleic acid extraction device of this creation can be directly operated by ordinary personnel in the general indoor environment. According to the nucleic acid detection method of this creation, the nucleic acid amplification reaction can be conveniently and easily completed to understand whether the biological sample has preset specifications. Nucleic acid virus, which greatly reduces the difficulty of nucleic acid detection, helps to carry out a large number of nucleic acid detection operations in a short time, and meets the requirements of patent application.

The above are only the preferred embodiments of the creation, and are not used to limit the scope of the embodiments of the creation. That is to say, all equal changes and modifications made in accordance with the scope of the patent application for this creation are covered by the scope of the patent for this creation.

10: Microcentrifuge tube

11: Lysis buffer

12: Biological samples

13: binding buffer

14: Washing buffer

15: Magnet

16: Professional pipette

17: Extraction buffer

20: Pedestal

21: Counterpoint area

22: The first moving part

221: Magnet end

23: The second moving part

231: Positioning gap

30: Rotating unit

31: bearing plate

311: Board Mark

32: bracket

33: Socket

34: handle

40: microcentrifuge tube

41: Lid

42: tube mark

45: Adsorption material

451: Slope

452: Marking Diagram

50: Dry bath heater

51: upper cover

501~504: steps

Figure 1 is a schematic diagram of the operation of the current magnetic bead extraction method.

Figure 2 is an exploded view of the created nucleic acid extraction device.

Figure 3 is a perspective view of another state of use of the created nucleic acid extraction device.

Figure 4 is an exploded view of the adsorption material and microcentrifuge tube used in the nucleic acid extraction device created.

Figure 5 is a flow chart of the author's nucleic acid detection method.

Figures 6A, 6B and 6C are schematic diagrams of the sample preparation of the nucleic acid detection method created.

Fig. 7A, Fig. 7B, Fig. 7C and Fig. 7D are schematic diagrams of the nucleic acid binding operation of the nucleic acid detection method created.

Fig. 8A, Fig. 8B, Fig. 8C and Fig. 8D are schematic diagrams of the cleaning operation of the nucleic acid detection method created by the invention.

Figures 9A, 9B and 9C are schematic diagrams of the nucleic acid amplification reaction of the nucleic acid detection method created.

20: Pedestal

21: Counterpoint area

22: The first moving part

221: Magnet end

23: The second moving part

30: Rotating unit

31: bearing plate

311: Board Mark

32: bracket

33: Socket

34: handle

40: microcentrifuge tube

41: Lid

42: tube mark

Claims (6)

A nucleic acid extraction device includes: a base with a pair of positions in the middle, the base is additionally provided with a movable first moving part, the first moving part is provided with a magnet end, and when the first moving part moves The magnet end moves in the alignment area; a rotating unit includes a bearing plate and two brackets erected on the base, the middle of the bearing plate has a bearing hole, and both ends of the bearing plate are pivotally connected to The support is on and can be rotated; a microcentrifuge tube is inserted into the bearing hole, the bottom of the microcentrifuge tube is located in the alignment area, and the magnet end can be close to the outer wall of the bottom of the microcentrifuge tube. According to the nucleic acid extraction device according to claim 1, the base further includes a movable second moving part, the second moving part is separated from the first moving part by 180 degrees, and the second moving part has a positioning notch, The movement of the second moving member can make the positioning notch be located at the center of the alignment zone, and the positioning notch can be located in the microcentrifuge tube to facilitate the contact between the magnet end and the outer wall of the microcentrifuge tube. The nucleic acid extraction device according to claim 1, further comprising an absorbent material that is cotton swabs, sponges, fibers, and materials that can absorb liquids. The absorbent material is inserted into the microcentrifuge tube and can absorb the materials used in the extraction process. Lysis buffer, binding buffer, washing buffer. According to the nucleic acid extraction device of claim 3, the shape of the adsorbent corresponds to the shape of the microcentrifuge tube, and the bottom has an inclined surface. When the adsorbent is inserted into the microcentrifuge tube, it will not contact the magnetic beads. According to the nucleic acid extraction device according to claim 4, the microcentrifuge tube has a tube mark, and the adsorption material has a marking image, and when the adsorption material is inserted into the microcentrifuge tube, the tube marking is aligned with the marking image. According to the nucleic acid extraction device according to claim 1, the rotating unit further includes a handle, the handle is installed on one side of the supporting plate, and the rotation of the handle can synchronously drive the supporting plate to rotate.

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