TWM664031U - Cell Culture Assistant - Google Patents

Abstract

A cell culture auxiliary device comprises a hollow first extension section, a hollow second extension section, a hollow third extension section, a first shoulder section, a second shoulder section and a gas channel. The second extension section is connected to the first extension section. The third extension section is connected to the second extension section. The first shoulder section is formed between the first extension section and the second extension section and is annular. The second shoulder section is formed between the second extension section and the third extension section and is annular, and the inner diameter of the second shoulder section is not less than the outer diameter of the first shoulder section. The gas channel is surrounded and defined by the inner circumference of the first extension section, the inner circumference of the second extension section and the inner circumference of the third extension section. The cell culture auxiliary device helps to improve the proliferation rate during cell culture.

Description

Cell Culture Assistant

The invention relates to a cell culture auxiliary device, in particular to a cell culture auxiliary device comprising a hollow extension section, a shoulder section and a gas channel.

Generally, when cell culture is performed, if you want to increase the cell proliferation rate, you usually need to add a promoter to the culture dish to promote cell growth. However, the promoter added above cannot be recycled and reused, so it often causes a considerable burden on the cost of cell culture.

Therefore, the purpose of the present invention is to provide a cell culture assistant that can overcome the shortcomings of the above-mentioned prior art.

Therefore, the novel cell culture auxiliary device includes: a hollow first extension section, a hollow second extension section, a hollow third extension section, a first shoulder section, a second shoulder section and a gas channel. The second extension section is connected to the first extension section in a length direction. The third extension section is connected to the second extension section in the length direction opposite to the first extension section. The first shoulder section is formed between the first extension section and the second extension section and is annular. The second shoulder section is formed between the second extension section and the third extension section and is annular, and the inner diameter of the second shoulder section is not less than the outer diameter of the first shoulder section. The gas channel is surrounded and defined by the inner circumference of the first extension section, the inner circumference of the second extension section and the inner circumference of the third extension section, and the gas channel extends in the length direction.

The effect of the novel device is that the cell culture assistant helps to increase the proliferation rate during cell culture.

The following is a detailed description of the content of this new model:

The novel cell culture auxiliary device comprises: a hollow first extension section, a hollow second extension section, a hollow third extension section, a first shoulder section, a second shoulder section and a gas channel. The second extension section is connected to the first extension section in a length direction. The third extension section is connected to the second extension section in a direction opposite to the first extension section in the length direction. The first shoulder section is formed between the first extension section and the second extension section and is annular. The second shoulder section is formed between the second extension section and the third extension section and is annular, and the inner diameter of the second shoulder section is not less than the outer diameter of the first shoulder section. The gas channel is surrounded and defined by the inner circumference of the first extension section, the inner circumference of the second extension section and the inner circumference of the third extension section, and the gas channel extends in the length direction.

Before the present invention is described in detail, it should be noted that similar components are represented by the same reference numerals in the following description.

The present invention will be further described with respect to the following embodiments, but it should be understood that the embodiments are only for illustrative purposes and should not be interpreted as limitations on the implementation of the present invention.

1 and 2 , a first embodiment of the novel cell culture auxiliary device includes a hollow first extension section 11, a hollow second extension section 12, a hollow third extension section 13, a first shoulder section 21, a second shoulder section 22 and a gas channel 9.

The second extension section 12 is connected to the first extension section 11 in a length direction L. The third extension section 13 is connected to the second extension section 12 in the length direction L opposite to the first extension section 11 .

In the first embodiment, the first extension section 11 , the second extension section 12 , and the third extension section 13 are each in the shape of a quadrangular column.

In the first embodiment, the first extension section 11 (including its inner circumference and outer circumference), the second extension section 12 (including its inner circumference and outer circumference) and the third extension section 13 (including its inner circumference and outer circumference) are all made of metal. In the first embodiment, the metal is copper.

The first shoulder portion 21 is formed between the first extension section 11 and the second extension section 12 and is in a rectangular ring shape. The second shoulder portion 22 is formed between the second extension section 12 and the third extension section 13 and is in a rectangular ring shape. The inner diameter of the second shoulder portion 22 is equal to the outer diameter of the first shoulder portion 21.

In the first embodiment, the inner edge of the first shoulder portion 21 is connected to the outer circumference of the first extension section 11, and the outer edge of the first shoulder portion 21 is connected to the outer circumference of the second extension section 12. The inner edge of the second shoulder portion 22 is connected to the outer circumference of the second extension section 12, and the outer edge of the second shoulder portion 22 is connected to the outer circumference of the third extension section 13. In this way, the relative positional relationship of the outer circumference of the first extension section 11, the first shoulder portion 21, the outer circumference of the second extension section 12, the second shoulder portion 22, and the outer circumference of the third extension section 13 is roughly in a step-like shape.

The gas channel 9 is surrounded and defined by the inner circumference of the first extension section 11, the inner circumference of the second extension section 12 and the inner circumference of the third extension section 13, and the gas channel 9 extends in the length direction L. In the first embodiment, the gas channel 9 is in the shape of an elongated cylinder.

Referring to FIG. 3 and FIG. 4 , a second embodiment of the novel cell culture auxiliary device is similar to the first embodiment, except that: in the second embodiment, the first extension section 11, the second extension section 12 and the third extension section 13 are each in a quadrangular pyramid shape. The inner diameter of the second shoulder section 22 is larger than the outer diameter of the first shoulder section 21.

Referring to FIGS. 5 to 8 , a third embodiment (as shown in the upper portion of FIG. 5 and FIG. 6 ), a fourth embodiment (as shown in the middle portion of FIG. 5 and FIG. 7 ) and a fifth embodiment (as shown in the lower portion of FIG. 5 and FIG. 8 ) of the novel cell culture auxiliary device each include a hollow first extension section 11, a hollow second extension section 12, a hollow third extension section 13, a hollow fourth extension section 14, a first shoulder section 21, a second shoulder section 22, a third shoulder section 23 and a gas channel 9.

The second extension section 12 is connected to the first extension section 11 in a length direction L. The third extension section 13 is connected to the second extension section 12 in the length direction L opposite to the first extension section 11 .

In the fourth embodiment, the outer peripheral surface of the first extension section 11 is a curved surface and convex outward. In the third embodiment and the fifth embodiment, the inner peripheral surface of the first extension section 11 is a curved surface and concave outward.

In the third embodiment, the fourth embodiment and the fifth embodiment, the first extension section 11 (including its inner circumference and outer circumference), the second extension section 12 (including its inner circumference and outer circumference), the third extension section 13 (including its inner circumference and outer circumference) and the fourth extension section 14 (including its inner circumference and outer circumference) are all made of metal. In the third embodiment, the fourth embodiment and the fifth embodiment, the metal is copper.

In the third embodiment, the fourth embodiment and the fifth embodiment, the first shoulder portion 21 is formed between the first extension section 11 and the second extension section 12 and is in the shape of a circular ring. The second shoulder portion 22 is formed between the second extension section 12 and the third extension section 13 and is in the shape of a circular ring, and the inner diameter of the second shoulder portion 22 is equal to the outer diameter of the first shoulder portion 21. The third shoulder portion 23 is formed between the third extension section 13 and the fourth extension section 14 and is in the shape of a circular ring, and the inner diameter of the third shoulder portion 23 is equal to the outer diameter of the second shoulder portion 22.

In the fourth embodiment, the inner edge of the first shoulder portion 21 is connected to the outer circumference of the first extension section 11, and the outer edge of the first shoulder portion 21 is connected to the outer circumference of the second extension section 12. The inner edge of the second shoulder portion 22 is connected to the outer circumference of the second extension section 12, and the outer edge of the second shoulder portion 22 is connected to the outer circumference of the third extension section 13. The inner edge of the third shoulder portion 23 is connected to the outer circumference of the third extension section 13, and the outer edge of the third shoulder portion 23 is connected to the outer circumference of the fourth extension section 14. As a result, the relative positions of the outer circumference of the first extension section 11, the first shoulder portion 21, the outer circumference of the second extension section 12, the second shoulder portion 22, the outer circumference of the third extension section 13, the third shoulder portion 23 and the outer circumference of the fourth extension section 14 are approximately stepped.

In the third embodiment and the fifth embodiment, the inner edge of the first shoulder portion 21 is connected to the inner circumference of the first extension section 11, and the outer edge of the first shoulder portion 21 is connected to the inner circumference of the second extension section 12. The inner edge of the second shoulder portion 22 is connected to the inner circumference of the second extension section 12, and the outer edge of the second shoulder portion 22 is connected to the inner circumference of the third extension section 13. The inner edge of the third shoulder portion 23 is connected to the inner circumference of the third extension section 13, and the outer edge of the third shoulder portion 23 is connected to the inner circumference of the fourth extension section 14. Thus, the inner circumference of the first extension section 11, the first shoulder portion 21, the inner circumference of the second extension section 12, the second shoulder portion 22, the inner circumference of the third extension section 13, the third shoulder portion 23 and the inner circumference of the fourth extension section 14 together surround and define the gas channel 9, and their relative position relationship is generally in a step shape. The gas channel 9 extends in the length direction L.

In the fourth embodiment, the gas channel 9 is defined by the inner circumference of the first extension section 11, the inner circumference of the second extension section 12, the inner circumference of the third extension section 13, and the inner circumference of the fourth extension section 14, and the gas channel 9 extends in the length direction L.

Referring to FIG. 5 , the third embodiment (as shown in the upper portion of FIG. 5 ), the fourth embodiment (as shown in the middle portion of FIG. 5 ) and the fifth embodiment (as shown in the lower portion of FIG. 5 ) can be combined into a combination including three novel cell culture auxiliary devices by sequentially connecting their respective gas channels 9 .

[ Cell culture assay ]

The combination of the third embodiment, the fourth embodiment and the fifth embodiment was combined, and 6 identical combinations were arranged in a 2×3 array. A culture dish inoculated with adult stem cells (ASCs) isolated from adipose tissue was placed 1.1 cm above and 2.3 cm below the array parallel to the experimental group. In addition, a culture dish inoculated with ASCs was used as a control group. The two culture dishes of the experimental group and the one culture dish of the control group were inoculated with the same number of ASCs.

Culture medium containing 10% fetal bovine serum was added to the two culture dishes of the experimental group and one culture dish of the control group, and the dishes were placed in a cell culture incubator (37°C, 5% CO ₂ , 20% humidity) for 24 h. The cell proliferation rate of ASCs in each culture dish was measured by MTT analysis at 0 h, 0.5 h, 2 h, 6 h, 16 h, 20 h, and 24 h of culture (n=3), and the measured values of the control group were used as the benchmark. The results are shown in Table 1 below. [Table 1] 0h 0.5 h 2 h 6 h Control group 1.000±0.043 1.000±0.014 1.000±0.020 1.000±0.007 Experimental group [above] 0.964±0.079 ( p =0.712) 1.022±0.004 ( p =0.205) 1.082±0.009 ( p =0.020) 1.079±0.024 ( p =0.035) Experimental group [below] 0.966±0.033 ( p =0.570) 0.996±0.021 ( p =0.882) 1.110±0.010 ( p =0.008) 1.084±0.006 ( p =0.001) 16h 20h 24h Control group 1.000±0.005 1.000±0.014 1.000±0.017 Experimental group [above] 1.112±0.019 ( p =0.005) 1.108±0.009 ( p =0.003) 1.239±0.014 ( p =0.000) Experimental group [below] 1.115±0.021 ( p =0.006) 1.123±0.022 ( p =0.010) 1.244±0.034 ( p =0.003)

As can be seen from Table 1, after culturing for 2 h to 24 h, the cell proliferation rate of ASCs in the experimental group was significantly higher than that in the control group (1.079 to 1.244 times, p ≤ 0.020).

In summary, the novel cell culture auxiliary device can affect the path of gas passing through the hollow first extension section 11, the second extension section 12, the third extension section 13, the first shoulder section 21, the second shoulder section 22 and the gas channel 9, thereby helping to increase the proliferation rate during cell culture, so the purpose of the novel device can be achieved.

However, the above is only an example of the implementation of the present invention, and it cannot be used to limit the scope of the implementation of the present invention. All simple equivalent changes and modifications made according to the scope of the patent application of the present invention and the content of the patent specification are still within the scope of the present patent.

11: First extension section 12: Second extension section 13: Third extension section 14: Fourth extension section 21: First shoulder section 22: Second shoulder section 23: Third shoulder section 9: Gas channel L: Length direction

Other features and effects of the present invention will be clearly presented in the embodiments with reference to the drawings, wherein: [Figure 1] is a three-dimensional view of the first embodiment of the present invention's cell culture aid; [Figure 2] is a cross-sectional schematic diagram of the first embodiment; [Figure 3] is a three-dimensional view of the second embodiment of the present invention's cell culture aid; [Figure 4] is a cross-sectional schematic diagram of the second embodiment; [Figure 5] is a three-dimensional view of the third embodiment, the fourth embodiment and the fifth embodiment of the present invention's cell culture aid; [Figure 6] is a cross-sectional schematic diagram of the third embodiment; [Figure 7] is a cross-sectional schematic diagram of the fourth embodiment; and [Figure 8] is a cross-sectional schematic diagram of the fifth embodiment.

11: First extension section

12: Second extension section

13: The third extension section

14: Fourth extension

21: First shoulder bone

22: Second shoulder bone

23: Third shoulder bone

9: Gas channel

L: Length direction

Claims (10)

A cell culture aid comprises: a hollow first extension section; a hollow second extension section connected to the first extension section in a length direction; a hollow third extension section connected to the second extension section in a direction opposite to the first extension section in the length direction; a first shoulder section formed between the first extension section and the second extension section and in an annular shape; a second shoulder section formed between the second extension section and the third extension section and in an annular shape, the inner diameter of the second shoulder section being not less than the outer diameter of the first shoulder section; and a gas channel defined by the inner circumference of the first extension section, the inner circumference of the second extension section and the inner circumference of the third extension section, the gas channel extending in the length direction. A cell culture aid as described in claim 1, wherein the inner edge of the first shoulder portion is connected to the outer peripheral surface of the first extension segment, the outer edge of the first shoulder portion is connected to the outer peripheral surface of the second extension segment, the inner edge of the second shoulder portion is connected to the outer peripheral surface of the second extension segment, and the outer edge of the second shoulder portion is connected to the outer peripheral surface of the third extension segment. A cell culture aid as described in claim 1, wherein the inner edge of the first shoulder portion is connected to the inner circumference of the first extension segment, the outer edge of the first shoulder portion is connected to the inner circumference of the second extension segment, the inner edge of the second shoulder portion is connected to the inner circumference of the second extension segment, and the outer edge of the second shoulder portion is connected to the inner circumference of the third extension segment. The cell culture aid as described in claim 3, wherein the gas channel is surrounded and defined by the inner circumference of the first extension section, the first shoulder portion, the inner circumference of the second extension section, the second shoulder portion, and the inner circumference of the third extension section. The cell culture aid as described in claim 1, wherein the inner diameter of the second shoulder portion is equal to the outer diameter of the first shoulder portion. The cell culture aid as described in claim 2, wherein the outer peripheral surface of the first extension section is a curved surface and protrudes outward. The cell culture aid as described in claim 3, wherein the inner peripheral surface of the first extension section is a curved surface and is concave outward. The cell culture aid as described in claim 1, wherein the inner circumferential surface of the first extension section is made of metal. The cell culture aid as described in claim 8, wherein the first extension section, the second extension section and the third extension section are all made of metal. The cell culture aid as described in claim 1 further comprises: a hollow fourth extension section connected to the third extension section in the longitudinal direction opposite to the second extension section; and a third shoulder section formed between the third extension section and the fourth extension section and in an annular shape, wherein the inner diameter of the third shoulder section is not less than the outer diameter of the second shoulder section.

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