TWM538398U - Micro-particle structure of dioscorea opposite fermentate - Google Patents

Description

Microparticle structure containing yam ferment

The present invention relates to a multilayered microparticle structure containing a fermented product of Dioscorea opposite , in particular to a multi-layer comprising at least one extract containing a yam fermentate from the inside out, and a multilayer layer of an outer structural layer located outside the essence layer. Particle structure.

The stomach is an organ that stores and digests food. It is connected to the esophagus and is connected to the duodenum. The position is located in the left upper abdomen of the human body. The main function of the stomach is to grind food into chyme while breaking down macromolecules in the food into smaller molecules for further absorption in the small intestine.

In recent years, with the westernization of diet and the increasing pressure on the lives of Chinese people, one in every four Chinese people suffers from stomach problems. The so-called stomach disease is actually a general term for many related diseases. The clinically common stomach diseases include, for example, the stomach. Esophageal reflux, gastritis, gastric ulcer, and stomach cancer, patients with stomach problems usually have upper abdominal discomfort, pain, indigestion, fullness after meals, stomach acid reflux, nausea, vomiting and other symptoms.

Gastritis refers to the inflammation or erosion of gastric mucosa. It can be divided into acute gastritis and chronic gastritis according to the pathological changes of gastric mucosal cells. In addition to abdominal pain, patients with gastritis often have nausea, vomiting, bloating, indigestion and poor appetite. Even weight loss, etc. Appropriate symptoms. Studies have pointed out that the main cause of gastritis is due to excessive gastric acid secretion, or the protective effect of gastric mucosa, which causes inflammation, erosion, ulceration and other diseases of gastric mucosal cells. The condition of chronic gastritis is usually relieved compared with acute gastritis, but the inflammation is longer and prone to recurrence, and the range of inflammation is wider. Clinical statistics show that patients with chronic gastritis are more than 20 times more likely to have stomach cancer than normal people, and their effects should not be underestimated!

Gastric cancer is caused by abnormal proliferation of gastric mucosal cells. Patients with early gastric cancer have no special symptoms, but as the cancer tissue expands and penetrates into the inner layer of the stomach wall, patients will begin to lose weight, loss of appetite, fatigue, difficulty swallowing, or Sustained vomiting, ascites and other symptoms, but so far, in addition to surgery, there is no other effective treatment. According to the statistics of the National Health Service of the Ministry of Health and Welfare of the Executive Yuan in 103 years, the number of people who died of stomach cancer in Taiwan was the 7th among the total number of deaths caused by all cancers, and the severity was obvious.

Yam ( Dioscorea opposite ) is a traditional health-care food, rich in active ingredients such as yam polysaccharide, mucin, dioscin, etc. Previously, the polysaccharides in yam have a good protective effect on gastric ulcer, but the way of using yam previously. The yam is cooked for the patient to eat, and the high temperature during cooking will destroy the enzyme components (such as amylase and digestase) in the yam, so that the yam eaten by the patient The yam polysaccharide content is low, which reduces its therapeutic benefit.

In view of this, the creators of this case have worked hard to create a multi-layered particle structure containing yam fermented products with gastrointestinal health effects. The microparticle structure contains high content of yam polysaccharide, which can improve stomach digestion and ease after taking. The symptoms of gastritis and gastric ulcer, and reduce the risk of stomach cancer, provide the dawn of treatment for patients suffering from stomach problems.

The main purpose of the present invention is to provide a multi-layered microparticle structure containing a Dioscorea opposite fermented product having a gastrointestinal health effect, the microparticle structure comprising at least an inner layer and an outer layer containing a yam ferment; An outer structural layer that is located outside of the elite layer.

In one embodiment of the multilayered particle structure of the present invention, the essence layer has a thickness of from 10 micrometers to 200 micrometers.

In another embodiment of the multilayered particle structure of the present invention, the outer structural layer has a thickness of from 30 micrometers to 200 micrometers.

In still another embodiment of the multilayered particle structure of the present invention, at least one of the elite layer and the outer structural layer may further comprise: an excipient, a moisture-proofing agent, a flavoring agent, a preservative, and a primary antibody. An oxidizing agent, a chelating agent, a penetrating agent, a lubricant, a coloring agent, a binding agent, an aqueous solvent, an oily solvent, a diluent, a stabilizer, an absorption retarder, a disintegrating agent, an emulsifier , a binder, a moisture absorbent, an immunomodulator, a bactericide, or a combination of the foregoing.

In still another embodiment of the multilayered particle structure of the present invention, the amount of the yam polysaccharide contained in the yam fermented product is 5 times higher than that of the unfermented yam.

In still another embodiment of the multilayered particle structure of the present invention, the activity of superoxide dismutase (SOD) contained in the fermented product of the yam is 5 times higher than that of the unfermented yam.

In still another embodiment of the multilayered microparticle structure of the present invention, the microparticle structure can be administered to an individual to reduce the gene expression of inflammatory chemokines IL-8 , TNFα , and IL1β of gastric cells, and to alleviate individual gastritis status.

In yet another embodiment of the multilayered microparticle structure of the present invention, the microparticle structure can be administered to an individual to increase the gene expression of Foxo3 and Sirt1 and reduce the risk of gastric cancer in an individual.

In still another embodiment of the multilayered particle structure of the present invention, the particulate structure can be used in food products, such as health foods.

The following is a description of the implementation of the creation according to the description of the creation of the creation; however, without departing from the spirit of this creation, the creation can be practiced in a variety of different forms, and the scope of protection of this creation should not be It is construed as being limited to those stated in the specification or drawings. In addition, in the drawings, the dimensions of the various items and regions may be exaggerated for clarity, and are not shown in actual scale.

1‧‧‧Multilayer particle structure

11‧‧‧Essence

12‧‧‧External structural layer

2‧‧‧ Jelly container

21‧‧‧ outer wall

22, 33‧‧‧ accommodating space

23‧‧‧ Jelly

24‧‧‧film

25‧‧ ‧ tablespoons

3‧‧‧Microcapsules

31‧‧‧First capsule body

32‧‧‧Second capsule body

34‧‧‧ 容层层

35‧‧‧Seal thin layer

36‧‧‧ accommodating slots

311‧‧‧ first joint

312‧‧‧

321‧‧‧Second joint

322‧‧‧ recess

T1, T2‧‧‧ thickness

1 showing a first aspect based partial cross-sectional perspective view of one embodiment of the present writing a multilayer structure of fine particles; microparticles schematic multilayer structure used in the present Creation jelly in order to sell in FIG. 2A and FIG. 2B lines, wherein FIG. 2A displaying the jelly products, using the 2B a schematic view showing a spoon to pick jelly for food display; schematic structure of multilayer microparticles in the microparticle using this creation capsules for selling of FIG. 3A through 3C system, wherein first 3A shows the microcapsule product, FIG. 3B shows a schematic cross-sectional view of the microcapsule, FIG . 3C shows a schematic view of the packaging of the microcapsule; and FIG. 4 shows the content of the yam polysaccharide contained in the yam before and after fermentation. Figure 5 shows the activity comparison of superoxide dismutase (SOD) contained in yam before and after fermentation. Figures 6A to 6E show the IL of gastric epithelial cells under different culture conditions. -8 , IL1β , TNFα , Foxo3 , and Sirt1 gene expression comparison chart.

"an," and "the" are used in the singular and plural terms. In addition, the terms "about", "about" or "nearly" as used herein mean substantially the difference between the stated value and the stated value within 20%, preferably within 10%, and more preferably 5%. Within. By "individual" is meant a mammal, which may be a human or a non-human animal.

Referring to Figure 1, there is shown a partial cross-sectional perspective view of an embodiment of the present invention, wherein the left side is a front view of the multilayered particle structure, and the particle structure is partially excavated, and the right side shows the excavated portion. Zoom in on the schematic. As shown in Fig. 1, the multilayered particulate structure 1 contains at least one essence layer 11 containing a yam fermentate and an outer structural layer 12 located outside the essence layer 11 from the inside out. In the present creation, the thickness T1 of the essence layer 11 is usually 10 μm to 200 μm, preferably 30 μm to 150 μm, more preferably 50 μm to 100 μm; and the thickness T 2 of the outer structural layer 12 is usually 30 μm to 200 μm. Micron, preferably from 50 micrometers to 150 micrometers, more preferably from 70 micrometers to 120 micrometers.

The yam fermented product contained in the multilayered microparticle structure of the present invention can be obtained by, for example, mixing a finely divided yam with a solvent, and then fermenting the mixed solution under appropriate conditions. For example, the finely divided yam and water may be mixed at a ratio of yam:water to a ratio of, for example, 1:2, and then subjected to fermentation at a temperature not lower than 15 ° C (for example, 25 ° C) for a duration of time. Not less than 2 hours (for example, 6 hours), and finally obtained by filtering out impurities. If necessary, the filtrate can be further dried to obtain a powder of yam ferment At the end, Eli has long-term preservation.

Compared with the unfermented yam, the yam fermented product contained in the multilayered microparticle structure of the present invention has a higher yam polysaccharide content. In general, the amount of yam polysaccharides of the yam fermented product contained in the microparticulate structure is at least 5 times higher than that of the unfermented yam, preferably higher than at least 6 times, and more preferably higher than at least 7 times. In addition, the yam fermented product contained in the multilayered particle structure of the present invention has a higher SOD activity than the unfermented yam, and is usually at least 5 times higher than the unfermented yam, preferably at least 7 times higher. Better systems are at least 10 times higher.

As mentioned above, the polysaccharide component in yam has a good protective effect on gastric ulcer. In addition, clinical studies have pointed out that if the activity of SOD in gastric cells can be increased, the oxidative stress of gastric cells can be effectively alleviated, thereby reducing the incidence of gastric mucosal lesions and gastric ulcers. Therefore, the multilayered particle structure of the present invention has a significantly higher polysaccharide content and SOD activity, and can effectively provide the effect of gastrointestinal health care.

In order to use the multilayered particle structure 1 of the present invention more flexibly, at least one of the essence layer 11 and the outer structural layer 12 may further comprise: an excipient, a moisture-proofing agent, a flavoring agent, a preservative, an antioxidant a chelating agent, a penetrating agent, a lubricant, a coloring agent, a binding agent, an aqueous solvent, an oily solvent, a diluent, a stabilizer, an absorption retarder, a disintegrating agent, an emulsifier, a binder, a moisture absorbent, an immunomodulator, a bactericide, or a combination thereof.

For example, at least one of the elite layer 11 and the outer structural layer 12 may contain at least one selected from the group consisting of corn starch, modified starches, sodium starch glycolate, and Gelatinized starches, dextrin, resin, hydroxypropyl cellulose, methyl cellulose (methylcellulose), carboxymethylcellulose, hydroxymethyl cellulose, hydroxypropylmethylcellulose, cellulose acetate, cellulose acetate phthalate, microcrystalline cellulose , glucose, lactose, sucrose, caramel, maltose, trehalose, oligosaccharide, glucomannan, palatinit, erythritol, mannitol, xylitol, sorbitol, lactose Alcohol, maltitol, polyethylene glycol (PEG), polyvinyl alcohol, polyvinylpyrrolidone (PVP), polyacrylate, polymethacrylate, carotene, Astaxanthin, lake, paprika, turmeric, annatto, vitamin E, pepper, mustard, wasabi, pepper, ginger, saffron, sorghum, capsaicin, clove oil (eugenol) ), cineol, zingerone, fat fried flavoring, pepper, olegano, carrageenan, prougin, gelatin, whey protein, casein , western wasabi, leeks, onions, parsley, sage, mint, savory, sesame seeds, vanilla, hazelnuts, poppy seeds, perilla, tansy, bitter, radish, celery , vanilla bunch (calquet garni), caldeepis, bee flower, basil, bay laurel, bay leaf, coriander, by garnish, cumin, garlic, dill, cinnamon, diced, nutmeg Nutmeg, nutmace, myristica, guar gum, tara gum, locust bean gum, tamarind, chamomile, juniper, lavender, marjoram, rosemary , lemon, star anise, tarragon, thyme, valerian, red pepper, anise, spirited vanilla (fenagreele), locust gum, pectin, yellow gum, galen gum, powdered pepper, garland mare salad (galama Sara), marinade, sichimi, spiced, Tabasco, curry powder, agar, alginic acid, carrageenan, xylan, propylene glycol, cardamom, lyme seed Gum, gum arabic, western yellow gum.

The above is merely illustrative of the usable components of the multilayered microparticle structure of the present invention. In actual use, those skilled in the art can arbitrarily adjust and select suitable components and amounts thereof as needed, and are not particularly limited herein.

The multilayer structure of the present invention can be used in different ways. For example, when used orally, the multi-layered particulate structure can be provided in different forms, for example: powders, capsules, microcapsules, lozenges (eg, sugar-coated tablets, double-layer ingots), pills, granules, powders, flooding Ointments, solutions, syrups, suspensions, emulsions, elixirs, capsules, and the like. In addition, the created multi-layered particulate structure can be added to foods or food additives, for example: added to puddings, jellies, pasta, water, tea, coffee, juice, soy milk, dairy products, chocolate drinks, juice drinks, sports Beverages, alcoholic beverages, lactic acid drinks, etc.

In one embodiment of the present invention, the multilayered particle structure of the present invention can be added to the jelly. Referring to Figures 2A and 2B, there is shown a schematic diagram of the use of the inventive multilayer particle structure for use in jelly for sale. As shown in FIG. 2A, the jelly container 2 has an outer wall 21 such that a hollow accommodation space 22 is formed therein, and the jelly 23 containing the multilayered particle structure 1 of the present invention is placed in the accommodating space 22, on the jelly container 2. A film 24 is covered to prevent the jelly 23 from tipping over during carrying. When the individual is eating, the film 24 can be torn as shown in Fig. 2B, and the edible jelly 23 is excavated from the jelly container 2 with a spoon 25.

In another embodiment of the present invention, the multilayered particle structure 1 of the present invention can be used in a health food. For example, the multilayered particle structure 1 of the present invention can be placed in a microcapsule body for convenient use by an individual. . Referring to Figures 3A through 3B, there is shown a schematic view of the present invention for the multilayered particulate structure to be sold in the form of microcapsules. Such as the first As shown in FIG. 3A, the microcapsule 3 is composed of a first capsule body 31 and a second capsule body 32. The first capsule body 31 and the second capsule body 32 have a first joint opening 311 and a second joint opening 321 respectively. The diameter of the first joint opening 311 is larger than the diameter of the second joint opening 321 so that the first capsule body 31 and the second capsule body 32 can be nested with each other to provide an intermediate accommodating space 33. It is placed in the accommodating space 33. In order to make the first capsule body 31 and the second capsule body 32 securely fit, as shown in FIG. 3B, a protrusion 312 is formed at the inner surface of the first capsule body 31, and corresponding to the second capsule body 32. A recess 322 is formed at the surface, and when the first capsule body 31 is engaged with the second capsule body 32, the protrusion 312 can be closely engaged with the recess 322.

At the time of sale, the microcapsules containing the present multi-layered particulate structure can be packaged in a sealed package for the individual to carry with them. For example, reference may be made to FIG. 3C, wherein the sealed package (not shown) includes a sheet layer 34, and the container layer 34 is provided with a plurality of receiving grooves 36 having independent openings on one side, and a The sealing sheet 35 on the other side of the sheet layer 34 is placed in the receiving groove 36. The package enhances the convenience of the individual to carry and access the multi-layered particle structure of the present invention, and reduces the risk of deterioration of the multilayered particle structure of the present invention in contact with moisture. When the individual is used, the sealing thin layer 35 can be torn off, and the microcapsule 3 containing the created multi-layered particle structure can be directly swallowed or used for drinking water.

The multi-layered particle structure of the present invention can be used at different frequencies, such as once a day, multiple times a day, or once a few days, depending on the needs of different individuals.

The effect of the present creation is further illustrated by the following examples. The embodiments are provided for illustrative purposes only and are not intended to limit the scope of the invention. The scope of this creation is as shown in the attached patent application.

[Examples]

Example 1: Preparation of Yam Ferment

Peel a fresh yam and chop the yam after peeling, mix the yam with clean reverse osmosis water at a solid to liquid ratio of 1:2, and place the mixed solution at 25 °C. The fermentation is about 6 hours. Thereafter, filtration was carried out with a mesh of 200 mesh to obtain a fermentation filtrate containing a yam ferment for use in the subsequent Examples 2 to 4.

Example 2: Analysis of the content of yam polysaccharide

The phenol-sulfuric assay was used to detect the content of yam polysaccharides. 100 μl of the unfermented yam solution, the fermentation filtrate obtained in Example 1, and the glucose standard solution of different concentrations were uniformly mixed with 0.5 ml of the 5% phenol solution; respectively, 2.5 ml of sulfuric acid was added, and evenly After mixing, it was allowed to stand for 20 minutes, and the absorbance of each solution at 490 nm was measured by a spectrophotometer. The regression equation was calculated by the absorbance measured by different concentrations of the glucose standard solution, and the unfermented yam was converted accordingly. The content of the yam polysaccharide contained in the solution and the fermentation filtrate obtained in Example 1 was shown in Fig. 4 .

It can be seen from the results of Fig. 4 that the content of the yam polysaccharide contained in the fermentation filtrate obtained in Example 1 is about 6.7 times higher than that of the unfermented yam solution, which shows that after fermentation, the yam polysaccharide in the obtained product can be effectively enhanced. Content, increase its benefits.

Example 3: Activity analysis of SOD

The activity of SOD was detected using a SOD activity test kit. First, prepare a 96-well plate, and add 25 μl of 1X SOD buffer, different concentrations of SOD standard solution, unfermented yam solution, and the hair obtained in Example 1 to different wells. Fermentation filtrate; in the well, add 150 μl of Master Mix and 25 μl of 1X Xanthine solution to each well, mix well and measure the solution by spectrophotometer. The absorbance at 450 nm is measured once every minute, and the data is collected continuously for 10 minutes. The regression equation is calculated from the absorbance measured by different concentrations of SOD standard solution, and the unfermented yam solution is converted accordingly. And the SOD activity in the fermentation filtrate obtained in Example 1, and the results are shown in Fig. 5.

From the results of Fig. 5, the SOD activity in the fermentation filtrate obtained in Example 1 was about 9.2 times higher than that of the unfermented yam solution, which showed that after fermentation, the SOD activity in the obtained product was effectively improved, and the improvement was provided. Antioxidant effect.

Example 4: Analysis of gene expression levels of IL-8 , IL1β , TNFα , Foxo3 , and Sirt1

The human gastric epithelial cells AGS were cultured in a 6-well cell culture dish; as shown in Table 1 below, the cells were divided into 6 groups and subjected to cell culture according to the conditions listed in Table 1; thereafter, phosphate buffered. solution was washed once, removing the culture supernatant, and adding 350 microliters of RNA extracted lysate to AGS cells containing the RNA, and then using a reverse transcriptase kit (available from ThermoFisher, model: SuperScript ^TM reverse Transcriptase kit) reverse transcription of RNA collected a DNA, to ABI StepOnePlus ^TM System (available from ThermoFisher Corporation) for IL-8, IL1β, TNFα, Foxo3, and gene expression levels Sirt1 be quantified, and the quantified results of the standardization of the control group This is shown in Figures 6A through 6E.

As shown in Figures 6A to 6C, the addition of the fermentation filtrate at a concentration of 2 mg/ml for 6 hours (i.e., experimental group B) significantly reduced the inflammatory chemokine IL of AGS cells compared to the control group. The gene expression of -8 , IL1β and TNFα , even under the culture conditions containing the lower fermentation filtrate concentration (ie, experimental group A), the gene expression of IL-8 in AGS cells was significantly reduced. This result clearly shows that the yam fermented product contained in the multilayered microparticle structure of the present invention can actually reduce the gene expression of inflammatory chemokines such as IL-8 , IL1β , and TNFα , and alleviate the state of inflammation in the stomach. Wherein, * indicates that the p value is less than 0.05 compared with the control group, with a significant difference; ** indicates that the p value is less than 0.01 compared to the control group, with a significant difference.

As shown in Fig. 6D, the addition of the fermentation filtrate at a concentration of 2 mg/ml for 6 hours (i.e., experimental group B) significantly increased the gene expression of Foxo3 in AGS cells compared to the control group; As shown in the figure, compared with the experimental group C, the addition of the fermentation filtrate with a concentration of 2 mg/ml for 24 hours (ie, the experimental group E) can significantly increase the gene expression of the Sirt1 of the AGS cells, and the above results clearly show that The produced yam fermented material can indeed increase the expression of genes such as Foxo3 and Sirt1 , and reduce the risk of gastric cancer in individuals. Wherein, * indicates that the p value is less than 0.05 compared with the control group, with a significant difference; ^# indicates that the p value is less than 0.05 compared with the experimental group C, with a significant difference.

The above detailed description is for the specific description of the embodiments of the present invention, and it should be understood by those skilled in the art that the above embodiments are only used to illustrate the present invention, and are not intended to limit the present invention. Variations, modifications, and equivalent implementations or modifications of the above-described embodiments are intended to be included in the scope of the present invention as long as they are within the spirit of the present invention.

1‧‧‧Multilayer particle structure

11‧‧‧Essence

12‧‧‧External structural layer

T1, T2‧‧‧ thickness

Claims (10)

A multi-layered microparticle structure containing a fermented product of Dioscoreo opposite , the multi-layered microparticle structure containing a fermentation product of Dioscoreo opposite contains at least an inner layer containing an yam ferment; and an external structure A layer, which is located outside the essence layer. A multilayered particulate structure comprising a Dioscorea opposite fermentate according to claim 1, wherein the extract layer has a thickness of from 10 micrometers to 200 micrometers. A multilayered particulate structure comprising a Dioscorea opposite fermentate according to claim 1, wherein the outer structural layer has a thickness of from 30 micrometers to 200 micrometers. The multi-layered microparticle structure containing the fermented product of Dioscorea opposite according to claim 1, wherein the essence layer and at least one of the outer structural layer further comprise: an excipient, a moisture-proofing agent, a flavoring agent, and a Preservative, an antioxidant, a chelating agent, a penetrant, a lubricant, a coloring agent, a binding agent, an aqueous solvent, an oily solvent, a diluent, a stabilizer, an absorption retarder, a collapse A powder, an emulsifier, a binder, a moisture absorbent, an immunomodulator, a bactericide, or a combination thereof. The multilayered microparticle structure containing the fermented product of Dioscorea opposite according to claim 1, wherein the amount of the yam polysaccharide contained in the yam fermented product is 5 times higher than that of the unfermented yam. The multi-layered microparticle structure containing the fermented product of Dioscorea opposite according to claim 1, wherein the activity of superoxide dismutase (SOD) contained in the fermented product of the yam is higher than that of the unfermented yam. Times. The multi-layered microparticle structure containing the fermented product of Dioscorea opposite according to claim 1, which is used for reducing the gene expression of inflammatory chemokines IL-8 , TNFα , and IL1β of gastric cells to alleviate individual gastritis. status. The multilayered microparticle structure containing the fermented product of Dioscorea opposite according to claim 1 is used for enhancing the gene expression of Foxo3 and Sirt1 to reduce the risk of gastric cancer in an individual. The multilayered microparticle structure containing the ferment of Dioscorea opposite according to any one of claims 1 to 8, which is used for foods. The multilayered microparticle structure containing the fermented product of Dioscorea opposite according to claim 9 is used in a health food.