TWM289977U - Storehouse-type tissue cultivation or microorganism fermentation device - Google Patents

Description

M289977 VIII. New Description: [New Technology Field] This creation is about a warehouse-type tissue culture or microbial fermentation device, especially a combination of food manufacturing automation technology, sterile room technology, automated storage technology and microbial fermentation technology. The integrated technology is used in tissue culture, micro-liquid, semi-solid, solid-state fermentation processes, and the use of storage principles to transfer the fermentation industry and tissue culture to mass production and manufacturing. [Prior Art] Since ancient times, humans have known how to use warehouses to store items, set up shelves in warehouses to make more efficient use of space, and cooperate with other protective measures to prevent stored goods from reaching rodents, insects, ants, moisture and moths. save time. Moreover, with the advancement of technology, mechanical automatic control technology has become more and more mature, and automated warehousing has emerged. This not only makes space more efficient, but also reduces the use rate and increases work efficiency by automatically storing equipment. However, the current automated storage device is only used to store goods, but the management work does not stop. The cost of the goods will be raised to the consumer, and if there is a logistics delay. In the case, the loss is even more difficult to estimate. To this end, ^If you can effectively use the convenience provided by automated warehousing and effectively use its storage time to work, it will not only reduce the waste of space costs, but also further improve the utilization of automated warehousing. Among them, tissue culture and microbial fermentation engineering is the work area of fully utilizing automated warehousing, because these work takes time, 5 M289977 space, and the control of the shovel of the shovel, if it can effectively use automated warehousing for management, It is bound to be mixed with this quality, and it can reduce the cost of the product and provide it to those who need it at a low price. The human plate has long known to use fermentation to produce the food or alcohol needed, such as the five autumn cereals or fruit wine, taro, bread fermentation, tea fermentation (black tea, Pu'er tea), soy sauce brewing soy sauce, making red glutinous rice, etc. Etc. These are all based on solid-sorrow fermentation or semi-fermentation, so humans have long known how to use the I-state fermentation process to make the food they need. However, the above traditional fermentation methods have several common points: 1. The fermentation fungi are mostly yeasts or molds; 2. Fermentation at normal room temperature and normal conditions; 3. Most of them are filled with disc blue or shallow dish-shaped containers. (fermentation); 4. Fermentation space does not need to be specially sterilized; 5. Naturally ventilate and dissipate by flipping (when the amount of medium is thick or thick) or standing, so that the fungus grows normally. Traditional solid-state fermentation methods are difficult to control in terms of quality control, whether quality, heat and gas transmission can not meet the required standards. For example, in the process of wine cellar production, not only yeast and gadolinium are produced, but also mixed with some acid and ridiculous. ^ of bacteria, such as esterification bacteria, lactic acid bacteria... The wine cellar manufacturing uses conditional control to screen high-quality yeast and sputum in the natural environment, but among them, it is almost no 6. M289977 sterilized raw material, and growth is also in the boudoir.卞Air is circulated with the outside world. So in the process of wine cellar production, not only the production of yeast and sputum, but also some acid and anaerobic bacteria, so the traditional solid-state fermentation method needs improvement. space. In modern times, many fungi products are fermented by mycelium. In the fermentation process of mycelium, they are generally divided into liquid fermentation and solid fermentation. In the liquid fermentation process, when the fermentation amount is small, the container is mostly used with a triangular flask. Large-scale fermentation uses a large fermentation tank; in the solid-state fermentation process, the container is used as a culture flask or a drum-mixed fermentation tank. The culture bottle method requires more disinfection procedures, consumes too much labor, and has less fermentation. The drum stirring type fermentation tank is limited by the heat dissipation and ventilation problems of the solid medium, and the yield of single fermentation is not large; and for the continuous heat dissipation of the medium, the rolling stirring is continued, the hyphae are continuously split, and the strain is allowed to stand due to Quiet growth, so the strain is less mature, therefore, there are process problems with less secondary metabolite production. In recent years, under the efforts of industry and academia, a variety of rotary agitated solid-state fermentation tanks have been developed, which can effectively control the growth requirements of temperature, humidity, and through-rolling during the growth process, but the production volume has not been able to increase. The heat dissipation problem cannot be effectively overcome. If the amount of the medium is too much, the internal temperature rises, causing the strain to die and the fermentation to fail. Such a fermentation tank rotates and stirs the medium in the tank to achieve the purpose of aeration, heat dissipation and mass transfer, but in the process of growth of the fungus, as long as the aeration is good and the heat dissipation is good, excessive agitation may affect the growth of the bacteria, such fermentation. The other disadvantage of the tank 7. M289977 is that if the spin-mixing stops, the bacteria can grow quietly, but the heat dissipation and ventilation problems will occur, so the fermented product cannot be mass-produced. At present, tissue culture and microbial liquid fermentation are large-scale production. The medium is used for the purpose of ventilation and mass transfer in the fermentation tank by rotary stirring or gas lift. The advantage is that the fermentation space can be designed and produced almost infinitely. The disadvantage is that the fermentation process is right. Infection with bacteria invades and is difficult to detect in the process. Although it is possible to observe the infection of bacteria by changing the mouth value, some bacteria infections will not change the value of 汕, and must wait for the output. Do test analysis; some fungi and some special requirements for special metabolites are not suitable for liquid fermentation process. Because liquid fermentation has a short growth period, many secondary metabolites of fungi cannot be produced because the strain is not mature enough. And after drying, there is not much solid product. Solid-state fermentation is currently available in both manual and semi-automatic ways. Manually, the medium is placed in a bottle-shaped container with a capacity of between about 2 〇〇 and 4 〇〇 gram. If the culture is too large, it will cause severe heat dissipation. Problems and ventilation problems, the flask is free of any agitation and unnatural air intake device; after the medium is cooked and cooled, it is filled into the culture bowl, then sterilized by cooling, inoculated, and the cap with the ventilation filter hole is covered, and then Place a number of culture flasks in a temperature-controlled space to allow the bacteria to grow in the bottle, but because there is no agitation and forced ventilation, and the inoculum (or pellet) is only distributed on the surface above the medium, the medium The interstitial transmission must be placed on a shocking machine during a specific day to allow the strain to spread between the culture media in a shocking manner, so that each medium can grow long bacteria. The ventilation method is 8 M289977. In the bottle, the mouth is simple or the cloth is cleaned. The air inside the bottle and the outside of the bottle are naturally 'all-in-one effect' and it is easy to cause infection due to the entry of bacteria, reducing the good rate. In the current cultivation process of the chapter, there are some benefits in the current

Cultivate by frame, ^ one boat passenger LV A Taiwei bag (solid material culture package) to do the cultivation of the fruit body of Xiang', Ganoderma lucidum, environmental management of the cultivation space, only temperature house degree, etc., for the aseptic management of the cultivation environment It is still paying attention and has not yet applied automated storage facilities to the development of fruiting entities. The mycelium culture still stays in the liquid fermentation tank, the culture flask and the small (5 liters to:) I state fermentation tank stage. Therefore, the prior art maintains the quality of the product and the production and production are not easy, so that the price of functional food remains high. In the current fermentation industry, the cultivation of mycelium has two common methods of liquid fermentation and solid fermentation. In the industrial production process of microorganisms, the liquid fermentation is carried out in a large fermentation tank, while the solid fermentation is carried out in a culture flask or a small-scale fermentation tank. Although the culture bottle can also be stored in a large amount by shelf method, its use is to increase the storage capacity of the culture bottle in the space, but the shelf itself does not see equipment such as semi-vibration ventilation and growth monitoring, and the traditional warehouse space is also lacking. The equipment is set up, so the whole work is more labor-intensive and more expensive. It is also difficult to make high-yield, high-performance fermentation applications. This creation is the application of automatic warehousing technology to tissue culture and microbial fermentation engineering. In the past, the storage device was only used for the storage of raw materials, semi-finished products or finished products of 9 M289977, and it was not manufactured. We use the management technology of aseptic space, plus the shelf and storage space with stirring, vibration, ventilation, temperature, humidity, pH control and growth monitoring function, and the management of fermentation conditions plus the design of shelf space. Fermentation tanks, as well as automated storage equipment, form a new automatic storage tissue culture and microbial fermentation unit. Therefore, the biggest difference from the previous storage equipment is that the past warehouse is just a place to store goods. This creation is to make the storage device no longer a warehouse, but a manufacturing plant, a high-capacity production equipment for tissue culture and fungus fermentation. [New content] The purpose of this creation is to provide a warehouse-type tissue culture or microbial fermentation device, which is a combination of food manufacturing automation technology, sterile room technology, automated storage technology and microbial fermentation technology. Tissue culture, microbial liquid, semi-solid, solid-state fermentation process, using the storage principle to transfer the fermentation industry and tissue culture to a technological innovation of mass production. In the past breeding process, molds and yeasts are strong mites in the environment and are ubiquitous in the natural environment. Therefore, the past fungus products such as wine cellar, cheese, bean drum, black tea, and Pu'er tea , red dragonfly, etc., is profit

Fall, but because of the temperature at the time, 'so there will be a little bit of bacteria on the medium, the humidity is set for the bacteria, so the strain is M289977 strong bacteria, will grow vigorously, other bacteria will become weak in this environment The number of colonies or colonies is not high, but it still affects the quality of the fermentation process. When the solid medium is sterilized, if the internal ventilation and heat dissipation conditions of the medium are good, there is no need to stir or flip, so that the hyphae naturally grows and becomes a mature strain, and then the secondary metabolites should be produced. In the process, if stirring and turning are required, the strain should be fully distributed in the medium, and the purpose of ventilation (no need for anaerobic bacteria) and heat dissipation. If the three elements are all available, the medium can be allowed to stand normally. growing up. With the upgrading of modern equipment and the maturity of automation equipment, the process of natural ventilation and fermentation in the boudoir can be effectively improved, so that the fermentation quality = stable, the output is larger, and the production scale of the solid state process is unprecedented. Solid-state fermentation is the most suitable method for artificial cultivation of fungi. Although it has been widely used in the cultivation of fruiting bodies, such as the use of space capsules (four), Ganoderma lucidum, Brazilian mills, Pleurotus eryngii, .., etc. In the cultivation of the body, solid-state fermentation faces many necks, can not be mass-produced and lacks effective sterile space management, and is easy to infect bacteria, resulting in low production yield. In the air, there are at most "fine g, mold and yeast. In the self-absent environment, they are strong bacteria, and artificial plants are planted with ', grass m', such as worms, burdock, mulberry and other high. The economical value of edible fungi is different from the flat-ground environment. If the cultivation environment is not properly managed, the cultivated fungi are easily infected by other invasive bacteria, and the fermentation cultivation fails. M289977 This situation is in the industry. Problems to be overcome. This creative department uses a modern automatic warehousing technology to match the design of the I-chamber space, to automate the mechanical fermentation tank and complete the sterilization = post-planting bacteria, by automatic access machine (or wheel conveyor, other handling machinery The fermenting container is placed on the shelf of the storage area, and each shelf has a growth monitoring device, a disturbing device, a vibrating device, and an independent air or oxygen supply: Lu set, providing all the bacteria needed in the fermentation process. If each fermentation vessel is applied to a solid medium, the best design is a flat-plate fermenter. The purpose is to increase the aeration of the medium in the fermentation vessel without affecting the aeration and heat dissipation of the medium, and to use a stirrer or The ventilation medium is placed in the tank to divide the medium in the tank into several zones to increase the effect of ventilation and heat dissipation. The shape can be round, square or other special design shape; if applied in liquid and semi-solid processes, fermentation The device has no shape limitation, but φ needs to install a temperature control tube in the fermenter to cool or increase the temperature. (If the fermenter is made of metal, the heat transfer coefficient is high, and the temperature control of the fermentation chamber can also be used to indirectly reach the fermenter. Temperature control). The inside of the fermentation vessel is provided with a stirring device, or the medium is separated into a plurality of culture zones by a ventilating powder and a heat separator. The separator must also have the functions of dispersing heat and venting; and the growth characteristics of the visible bacteria are used in ventilation. Natural air convection or direct introduction of air or oxygen. Of course, a certain number of culture bottles can also be placed on the troughs. The automatic access machines are sent to the storage area for storage, but this method is more labor-intensive, and the infection rate of the bacteria is high, and the production efficiency is poor. [Embodiment] 12 M289977 Please refer to Figure 1 and Figure 2. The biological fermentation 胄 所 % % % % 培养 培养 培养 培养 培养 培养 培养 主要 主要 主要 主要 一 一 一 一 一 一 一 一 一 一 一 一 一 一 一 一 一 一 一 以 以 以 以 以 以Equipment, such as $ 〇 ^ ^ u construction 'with various guides, heart pressure reduction bacteria, plasma reduction, UV sterilization ·..·.·., one to maintain sterility before fermentation and fermentation; - Automated storage device 2... It has a storage structure, a storage/removal state, a storage, a storage, a material handling and conveying equipment, a medium conditioning, and a filling automation device, all of which are tissue culture and microbial fermentation. Do integrated design, manufacturing; a shelf 3 (also known as Cangjian)... Made of high-capacity, anti-oxidation, anti-six temperature, and sound-resistant materials, providing the storage compartment 31 stored in the fermentation tank 5 described later.丨 仓 仓 射 进行 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Set culture, the store The grid 31 can carry the required space for the fermenting tank by carrying the platform 32 or other carrying manners; the carrying platform 32 of each shelf 31 of the shelf 3 is provided with an intake pipe and a joint, and the fermentation tank placed in each of the storage compartments 31 5 linkage, the sterile air is transported into the fermentation tank 5 for use by the bacteria; another embodiment is that the air in the fermentation tank and the air in the sterile room are naturally convected without passing through the intake line; 13 M289977 The storage platform 32 of the storage unit αw α 31 is equipped with a vibrating device or a suspicious stirring device, so that the nutrient base in the tank can produce sufficient quality transmission effect, and the rotary fermentation can be set on the fermentation tank 5 to the 袢 袢 袢 device. , or by gas lift to achieve the quality and communication "six fruit S kinds of knowledge of the way to provide only the fermenter space for the storage space, the static placement method allows the microbes to grow naturally · the shelf 3 per cell storage compartment 31 is installed temperature Humidity pH value,

Oxygen content monitoring, etc. 33 installation, plug-in version said # clothing to keep the growth environment of the bacteria in the fermentation tank 5 in an optimal state; temperature, sensational pH value and related monitoring devices, can also be shelf Or each fermentation storage area is a unit; Referring to FIG. 1 , the foregoing shelf 3 may not be provided with the carrying platform 32, and the hanging plate 32a is placed on the shelf, and the movable storage compartment 31a is hung on the hanging plate 32a for convenience. It is placed in the shelf 3 by means of a change. A sterile air device 4 - maintains the air entering the storage space i and the fermentation tank 5 described later in a sterile state; the fermentation tank (not shown) is placed in a breeding area for liquid fermentation The process is mainly (or solid), and the inoculum (or inoculum) with the number of colonies up to the set standard is implanted into the medium of the fermentation tank in the storage tank, and the inoculum is inoculated through the inoculation hole of the inoculation machine or the fermentation tank. Larger amount of liquid or solid medium; Fermentation tank 5 (also known as bioreactor)---Universal type is flat-shaped design's shape is round, square, rectangular; round fermentation tank 5 culture 14 M289977 is mainly based on rotary stirring. The square and rectangular shapes are mainly vibration. The ventilation and heat-dissipating partitions 51a and 5ib are arranged in the groove, which are divided into a general-type grid 5U and a hollow-type grid 51b. The solid medium is divided into several zones. Its function is to maintain the medium for good heat dissipation and ventilation, and the tunnel type can be rotated or vibrated. The foregoing device is in a state in which the medium is in a solid state, and if the medium is in a liquid state or a semi-solid state, rotary agitation is the best quality transfer method. Although the gas lift type is also a method of φ, if the cultured microorganism is anaerobic or the air demand is small, the gas lift method is not suitable for use. The ideal design of the stirring function of the fermentation tank 5 is that if the power is transmitted from the bottom and the stirring blades are driven by the motor 6 and the bearing, the contamination of the oil seal gap is likely to contaminate the fermented material, and if there is leakage, the fermented material and the fermentation tank 5 are contaminated. Therefore, if the power is transmitted from the bottom of the fermentation tank 5, a bearingless design should be used, and the rotor driven by the motor 6 is mounted in the load-bearing platform 32 of the shelf storage compartment 31 by the principle of magnetic homogeneity and opposite-sex attraction. Or a number of powerful magnets 61 of the wheel, the wheel or the gear unit, and the agitating blades 52 in the fermentation tank 5 are also equipped with a number of powerful magnets, which will drive the stirring in the fermentation tank 5 when the lower motor 6 rotates. The blade 52 is rotated, agitated, and the medium in the tank. • The power source can be driven by the motor 6 or by a separate gas circuit. The pneumatic motor is used to drive the wheel, and then the wheel or gear drives the rotation of the agitating blade 52 in the fermentation tank 5. The power can also be transmitted from above. Because there are vent holes, inoculation holes, 15 M289977 sample holes, observation holes and related facilities (not shown), the power source is as far as possible. Don't design from the top. In order to increase the space efficiency and reduce the equipment cost, and avoid the configuration of each motor 6 in each fermentation tank 5, several runners or gears should be driven by the motor/belt to save space for storage. The fermenting tank 5 is functionally distinguished as follows: a. Generally, the fermenting tank medium is inoculated into the fermenting tank for fermentation, or the medium is placed in the fermenting tank and then inoculated, and the tank has a stirring blade or a ventilating heat-dissipating partition to suit the Cultivate space and environmental conditions to grow cells or microorganisms in the tank. b. The composite fermenter is constructed like a pressure cooker (fast pot), and the medium is sterilized in the pot. > Boiled one > Cooling _ > Inoculation One > Fermentation, in addition to the high pressure cooking pressure relief hole and safety valve and other devices, more inoculation holes, sampling holes, monitoring devices, in the tank cover or tank to install air intake holes, to provide cell or microbial growth If it is an anaerobic microorganism, the entire tank can be sealed. The right-handed solid-sorrow culture process is adopted. The fermentation tank needs to be designed in the inner and outer tanks. The inner tank is placed in the medium tank. The outer tank is filled with water or other liquid. The bottom of the tank is treated with heat source and heated to treat the medium and sterilize. After cooling, it is inoculated. The tank cover can also be designed with x inner and outer covers. The inner cover is made of air filter effect and ventilation material. The heat effect of the ventilation is 16 M289977. When the high temperature and high pressure cooking is completed, the outer cover can be removed and the inner cover can be used for ventilation. The heat dissipation area is naturally ventilated to provide a better growth environment for cells or microorganisms. The same effect can be achieved if the boiler steam is introduced into the fermentation tank for cooking and sterilization. If the air is guided to the tank by an unnatural external force, it can be designed with a single cover. The air inlet or the steam (4) hole of the upper cover becomes the air hole. • A... In the solid medium or liquid medium, the solid medium mainly provides the carbohydrates (carbon sources) required for the growth of microorganisms, and the selection must be good in addition to the rich carbohydrates and related nutrients. Moisture retention, water resistance of dipping and the breathability of each medium are not easy to adhere, and resistance to mixing, etc., and the consideration in the strength is also very important. Monosaccharides and polysaccharides as well as the temple powder can provide the carbon source needed for this creation, and 10:, and the classification is basically based on whole grains, such as coix seed, sorghum, millet, barley, buckwheat, beans, potato (sweet potato). , horse yam), can be a solid medium; because the temple powder must be saccharified to become a microbial nutrient, therefore, ♦ before the □ & medium before treatment, must be given the right amount of sugar , Provided • Required for the initial carbon source of microbial growth. According to most studies, the composite carbon source (complex composition and enrichment of fibrin hemicellulose) is conducive to the growth of mycelium and the biocracking of bioactive materials. The reasons may be as follows. The composite carbon source contains Other nutrients, 17 M289977 such as a variety of vitamins and trace elements, of which cellulose is the precursor of the synthesis of extracellular polysaccharides in the sputum and scorpion, which in turn promotes the accumulation and extraction of extracellular polysaccharides. According to the census, Yiren has the effect of reducing jk fat, and also contains water-soluble fiber which is quite rich in ugly fields, and very rich B-type vitamins (such as vitamin Β1, μ» η, and Β3), so it is a Very good solid medium' contributes to the deep fermentation of g filaments and the formation of bioactive substances.

The nitrogen source is an indispensable raw material for the synthesis of protein nucleic acids and metabolites by microbial cells. Acid, therefore, the organic nitrogen source is better than the single organic nitrogen source. The cells cannot use inorganic nitrogen to synthesize some essential amino nitrogen, which is the most suitable nitrogen source for microbial growth. Compound organics such as yeast powder, yeast extract, protein, bean cake powder, peanut cake powder, rice bran, wheat bran, etc., are more suitable for the fermentation of the bacteria than the single nitrogen source.

The vitamin B group, especially vitamins (thiamine), is an essential vitamin for the growth process of mycelial cells. Add vitamins (4) and (4) to the growth of the bacteria in the medium. If the vitamins are lacking, the bacteria will grow slowly or stop growing. The BT (L-Carnitine) can promote the growth of bacteria, which can increase the resistance to bacteria and the resistance of the environment. Compounds supplemented with magnesium (Mg) and potassium (1) and phosphorus (P) are required for the growth of bacteria to be converted into organic trace elements, and promote the growth of mycelium. In addition, 'Selenium (Se) is a mineral with anti-cancer and anti-oxidation functions; remarks () eight minerals with antioxidant and increased ability to function; anti-oxidation, anti-oxidation, M289977 decontamination; chromium (Cr) to lose weight , lowering blood sugar, etc., in the growth formula of the bacteria, adding trace element compounds having specific effects to the human body, or inorganic trace elements or yeast powder containing trace elements, for conversion to organic trace elements after consumption by the bacteria' The product has more functions and even therapeutic effects on the human body. Ome shell, Lgneous Rock, Capra Hircus L, Coral lium Japon icum Kishinouye, keel Drs - Draconis Coloratus, Dens Draconis, Pumice Calcium Carbonate, Phosphate Feed, Activated Carbon, these minerals are ground into powder and mixed in solid medium (less than 5% by weight) to increase water absorption and avoid sticking of the medium, increasing the permeability of the medium. At the same time, minerals are added. Water is to keep the solid medium in a damp state, providing a suitable environment for the growth of bacteria. The moisture content and relative humidity are revised according to different strains and different culture environments. The addition of trehalose (CuHuOu) increases the moisturizing effect of the medium and the environmental resistance of the fermented strain. In addition, the liquid used in liquid fermentation is A. I qk Luji, the carbon source used in the sputum is mainly sucrose, glucose or molasses. In recent years, the medium of the temple powder has been gradually incorporated into it as a carbon source. In the deep fermentation of Ganoderma lucidum, the biomass, crude polysaccharide and crude ginseng acid content are high. 19 M289977 Some formulations of solid-state culture can be used for other nitrogen sources, vitamins, minerals and other nutrients, but the dosage and concentration should be modified separately. The high concentration of carbon source (high osmotic pressure) has an inhibitory effect on the growth of the cells; the concentration of nitrogen source has little effect on the fermentation cycle, but affects the yield of polysaccharides and secondary metabolites of Ganoderma lucidum. The solid medium used for culturing JHL or test tubes is mainly acacia or broccoli, and other nutrients are added. Refer to Figure 3 for the operation of the above device, which includes: 3. Pre-treatment of the medium, including the nitrogen source of the medium storage tank, the carbon source, the desired protein, vitamins, minerals and other additives; b · Cooking and sterilizing the medium, then cooling; c. Selecting the strain to be produced, cultivating the culture dish, and then expanding the flask to enlarge the culture; d· amplifying the culture solution to the liquid fermentation tank (if the inoculum is small, available) Triangular flask culture), forming a seed inoculum; can also be inoculated as a seed source by solid inoculation; e · inoculation by inoculation machine; f · cleaning and sterilization of culture vessels; avoiding inoculation with medium automatic feeder After the medium is loaded into the vessel; h· transport the incubator with an automatic access machine or conveyor belt; i· enter the sterile room automatic storage device; J.. sterilize the sterile room (before the pre-operation), · 20 M289977 k· Carry out growth observation and sampling inspection at any time; 1. If necessary, carry out the necessary treatment measures; ID·I will be the result of the growth of the finished fermentation products. Automatic access machine or conveyor belt (or its η·drying, grinding; 〇. processing, packaging; Ρ·delivered to the finished product storage area; q. can be sampled and analyzed after drying, grinding, r; record the optimal component content and optimal culture conditions. The steps of d, e, f, g can also be changed as follows. · d. Then enlarge the ® solution to the liquid fermentation tank culture (if the inoculum is small, the triangle can be used)缻 or other small container culture), forming a seed inoculum; e. Cleaning and sterilizing the culture vessel; f· Loading the medium into the phoenix with a medium automatic feeder; g· Inoculation with a vaccination machine Fourth, the author uses the high-pressure culture direct fermentation process, and the difference with Figure 2 is that the medium is directly boiled and sterilized in the high-pressure culture vessel in step bl, and the sterilizing cooking vessel is the fermentation culture vessel, eliminating the general culture. The step of cleaning and sterilizing the ware; therefore, the step e 丨 can be directly inoculated in the culture vessel to save the step of adding the inoculated medium to another fermenter JHL. 21 M289977 The profit content is expressed by the automated process. In the implementation, the warehouse: fermentation is the main core, depending on the species and the subjective and objective environment differences, the connection of each process can be completed in a fully automatic, semi-automatic or manual manner; Automatic storage device for the shelf (storage rack), the storage of the material (fermentation tank),

The material must be taken out or stored by automatic access to the handling equipment; the automatic storage device of the mobile or rotating shelf (storage rack) can move the material (fermentation tank) to the designated position by moving or rotating. The storage or removal of materials by human or mechanical means; or the operation of access by automatic access handling equipment. 0 Storage-type fermentation method, which is the most effective use of environmental management to achieve space. Zoom in or out as needed. The detailed description above is a detailed description of one of the possible embodiments of the present invention. The present invention is not intended to limit the scope of the present invention. Any equivalent implementation or modification that is not departing from the spirit of the present invention should be included in The patent scope of this case. BRIEF DESCRIPTION OF THE DRAWINGS Referring to the following detailed description of a preferred embodiment of the present invention and the accompanying drawings, the technical contents of the present invention and its functions will be further understood; the drawings relating to the embodiment are: Figure 1 is the device architecture diagram of the creation; Figure 1 is a variation diagram of the shelf storage grid of the creation; Figure 1A is an enlarged view of the related structure of the fermentation tank; 22 M289977 Figure 2B is another fermentation of the creation Slot structure diagram; Figure 3 is a general flow chart of the creation; and Figure 4 is a flow chart of the direct fermentation of high pressure culture JHI for the creation. [Main component symbol description] 1 Storage space 2 Automated storage device 3 Shelf (warehouse) 31 Storage compartment 32 Load platform 33 Monitoring device 4 Sterile air device 5 Fermentation tank (bioreactor) 51a Partition 51b Partition 52 Mixing blade 6 Motor 61 powerful magnet 23

Claims (1)

M289977 IX. Scope of application: i A type of storage (4) culture or microbial delivery, including: , layer storage room, can be divided into different work areas, respectively, breeding, medium therapy, sterilization, cooling, inoculation, filling, fermentation Waiting for work · Warehousing and arranging 'automatic mechanization of different working areas of the storage space & door storage/removal, material handling and transportation, medium preparation, inoculation, filling, capping, etc.; A shelf is placed in the storage space, and is provided with a plurality of health cells to provide a space for tissue culture or microbial fermentation; the cell can be extended in a strip shape to form a tunnel mode: a sterile air device, maintained The air entering the storage space is in a sterile state; a fermenter is placed in the storage compartment, and the aeration and quality transmission functions required for growth are achieved by rotating stirring or vibrating or standing to carry out the bacteria species. Culture or fermentation. 2. For the storage type tissue culture or microbial fermentation device as described in the application (4), the breeding inoculum is planned to have a breeding area, and the breeding area is provided with a fermentation tank, which may be a liquid seed tank or The solid bacteria trough is used to culture the inoculum or the solid inoculum. When the number of colonies reaches the set standard, the inoculum is inoculated into the general fermentation tank medium or inoculated first, and then filled into the fermentation tank. 3. Warehousing tissue culture or microbial 24 M289977 fermentation apparatus as described in the patent application section, wherein the storage space is provided with various sterilization equipment such as high temperature and high pressure sterilization, plasma, and external sterilization of bacteria and bacteria to achieve a storage space of Requirements for sterilisation and sterile space. The warehousing n-culture or microbial fermentation device described in the stipulations of the Shenyue patents, the towel (4) is provided with a load-bearing platform at the bottom of the compartment for providing accommodation space for other functional devices. 5. For the application (4), the fourth item of the item (4) _ culture or microbial t-fermentation device, wherein the storage compartment is provided with an intake line and a joint, so that the fermentation tanks of each storage compartment are transported and transported to each other. In order to transport the flawless air into the tank for bacteria use. 6) The patented tissue culture or microbial I fermentation apparatus described in claim 1, wherein the storage compartment has a vibrating device or a rotating stirring device. 7. If the patent application scope 1, storage _ culture or microbial hair fermentation equipment, the storage of the endangered 汊 汊 storage in the second room with temperature, humidity, acid test value, including cover s 虱 光线, light, etc. Monitoring device. 8. If the application of the patent range j jth fermentation equipment 4 warehouse mistype tissue culture or microbial fermentation device, wherein the fermentation uh ^ and straight hundred stir the # blade, the stirring blade is equipped with a powerful magnet, and the Μ storage 袼 is also installed below The powerful magnet, when the horse, the 喆 喆 ” ” ” ” ” ” ” ” ” ” ” ” ” ” ” ” ” ” ” ” ” ” ” ” ” ” ” ” ” Cut the table leaven 9. If you apply for the patent scope! Layer storage tissue culture or microbial 25 M289977 hairy coat set 'The fermentation tank is provided with a ventilating heat-dissipating partition for dividing the medium into several areas in an appropriate amount to increase ventilation and heat dissipation. 10.1· The warehouse-type tissue culture or microbial fermentation device described in item i of the patent scope, wherein the cargo 牟~, /, beech wood is solid-solid, or can be mobile or rotary to provide a storage tank for storage. In the face of the day, the space efficiency is optimized. For example, when applying for a patented sample fermentation device, it is done manually. The connection between the storage cultures or micro-devices described in item 1 can be fully automated, semi-automatic or 26