TWI897463B - Bioparticle contactless processing apparatus - Google Patents

Abstract

The present invention provides a bioparticle contactless processing apparatus, which includes an accommodating device and a triggering device. The accommodating device includes a light sensing structure, a mating structure spaced apart from the light sensing structure, and a frame arranged between the light sensing structure and the mating structure. The frame has two working segments having a first opening and a second opening that is smaller than the first opening, and the first opening and the second opening are respectively arranged on two opposite ends of the two working segments. The triggering device is disposed at a position corresponding to that of the frame. When at least one bioparticle is transferred into the frame by passing through the first opening and has a size larger than the second opening, the triggering device is configured to trigger an outer layer of the at least one bioparticle to have a predetermined permeability higher than an original permeability thereof.

Description

Non-contact biological particle treatment equipment

The present invention relates to a biological particle processing device, and more particularly to a non-contact biological particle processing device.

Existing bioparticle processing devices can move bioparticles by applying light. However, these devices struggle to accurately control the bioparticles at a fixed point during processing. The inventors, believing these limitations could be addressed, conducted intensive research and applied scientific principles to develop the present invention, which is both rationally designed and effectively addresses these limitations.

The present invention provides a non-contact biological particle processing device that can effectively improve the defects that may occur in existing biological particle processing devices.

The present invention discloses a non-contact biological particle processing device, which includes: a container for containing a liquid sample containing a plurality of biological particles and a transfection substance, and the container includes: a photosensitive structure having a first substrate, a first electrode layer formed on the first substrate, and a photoelectric layer formed on the first substrate; a matching structure spaced apart from the photosensitive structure; wherein at least one of the photosensitive structure and the matching structure is transparent. The matching structure includes a second substrate and a second electrode layer formed on the second substrate, and the second electrode layer faces the light sensing structure; and a frame is arranged between the light sensing structure and the matching structure; wherein the frame has two working sections facing each other, and the opposite ends thereof are respectively formed with a first opening and a second opening smaller than the first opening; wherein at least one of the plurality of biological particles is defined as a target biological particle having a diameter greater than The second opening has a particle size, and the cell surface layer of the target biological particle has an initial permeability; a light capture device facing the containing device; wherein the light capture device can be used to form a first dielectrophoretic pattern on the light sensing structure, so as to drive the target biological particle through the first opening and enter between the two working sections through the first dielectrophoretic pattern; and a trigger device, which is arranged corresponding to the frame; wherein the trigger device can be used to trigger The cell surface layer of the target biological particle located between the two working sections is configured to have a predetermined permeability greater than the initial permeability. When the target biological particle is captured between the two working sections, the light capture device can be used to form a second dielectrophoretic pattern on the light-sensing structure, thereby driving the transfection substance through the second opening and into the space between the two working sections through the second dielectrophoretic pattern to achieve a transfection operation.

The present invention also discloses a non-contact biological particle processing device, which includes: a container for containing a liquid sample containing a plurality of biological particles, and the container includes: a light-sensing structure having a first substrate, a first electrode layer formed on the first substrate, and a photoelectric layer formed on the first substrate; a matching structure spaced from the light-sensing structure; wherein at least one of the light-sensing structure and the matching structure is transparent, and the matching structure includes a A second substrate and a second electrode layer formed on the second substrate, wherein the second electrode layer faces the light sensing structure; and a frame disposed between the light sensing structure and the matching structure; wherein the frame has two working sections facing each other, and a first opening and a second opening smaller than the first opening are formed at opposite ends thereof; wherein at least one of the plurality of biological particles is defined as a target biological particle having a particle size larger than the second opening, and the size of the target biological particle is The cell surface layer has an initial permeability; a light capture device facing the containing device; wherein the light capture device can be used to form a first dielectrophoretic pattern on the light sensing structure to drive the target biological particles through the first opening and enter between the two working sections through the first dielectrophoretic pattern; and a triggering device, which is arranged corresponding to the frame; wherein the triggering device can be used to trigger the cell surface layer of the target biological particles located between the two working sections to cause the cells to The outer layer has a predetermined permeability greater than the initial permeability, thereby causing the target biological particle to proliferate an exosome that passes through the outer layer of the cell. When the target biological particle is captured between the two working sections and the exosome proliferates, the light capture device can be used to form a second dielectrophoretic pattern on the light-sensing structure, so that the second dielectrophoretic pattern drives the exosome to pass through the second opening between the two working sections and leave the two working sections, thereby achieving a purification operation.

The present invention also discloses a non-contact biological particle processing device, which includes: a container for containing a liquid sample containing a plurality of biological particles, and the container includes: a light-sensing structure having a first substrate, a first electrode layer formed on the first substrate, and a photoelectric layer formed on the first substrate; a matching structure spaced apart from the light-sensing structure; wherein at least one of the light-sensing structure and the matching structure is transparent, and the matching structure includes a second substrate and a second electrode layer formed on the second substrate, and the second electrode layer faces the light-sensing structure; and a frame disposed between the light-sensing structure and the matching structure. structures; wherein the frame has two working sections facing each other, and a first opening and a second opening smaller than the first opening are formed at opposite ends thereof; wherein at least one of the plurality of biological particles is defined as a target biological particle having a particle size larger than the second opening, and the cell surface layer of the target biological particle has an initial permeability; and a triggering device, which is arranged corresponding to the frame; wherein, when the target biological particle passes through the first opening and enters between the two working sections, the triggering device can be used to trigger the cell surface layer of the target biological particle so that the cell surface layer has a predetermined permeability greater than the initial permeability.

In summary, the non-contact biological particle processing apparatus disclosed in the embodiments of the present invention utilizes the frame and the triggering device to cooperate with each other and be matched with the light-sensing structure of the container device, so that the frame can be used to locate the target biological particles. Furthermore, the triggering device can increase the permeability of the cell surface layer of the target biological particles as needed, thereby facilitating the precise processing of the target biological particles (such as the transfection process or the purification process).

To further understand the features and technical contents of the present invention, please refer to the following detailed description and drawings of the present invention. However, such description and drawings are only used to illustrate the present invention and are not intended to limit the scope of protection of the present invention.

The following describes the implementation of the "non-contact biological particle processing device" disclosed in the present invention through specific embodiments. Those skilled in the art can understand the advantages and effects of the present invention from the contents disclosed in this specification. The present invention can be implemented or applied through other different specific embodiments, and the details in this specification can also be modified and changed based on different viewpoints and applications without departing from the concept of the present invention. In addition, the drawings of the present invention are only for simple schematic illustrations and are not depicted in actual size. Please note that the following embodiments will further explain the relevant technical content of the present invention in detail, but the disclosed content is not intended to limit the scope of protection of the present invention.

It should be understood that while terms such as "first," "second," and "third" may be used herein to describe various components or signals, these components or signals should not be limited by these terms. These terms are primarily used to distinguish one component from another, or one signal from another. Furthermore, the term "or" as used herein may include any one or more combinations of the associated listed items, as appropriate.

[Example 1]

Please refer to Figures 1 to 6 , which illustrate a first embodiment of the present invention. As shown in Figures 1 to 3 , this embodiment discloses a non-contact biological particle processing apparatus 100 , which includes a container 1 , an AC device 2 electrically coupled to the container 1 , a light capture device 3 facing the container 1 , and a trigger device 4 disposed within the container 1 , but the present invention is not limited thereto. For example, in other embodiments not shown in the present invention, the non-contact biological particle processing apparatus 100 may omit at least one of the AC device 2 and the light capture device 3 , depending on actual needs. For example, the combination of the container 1 and the trigger device 4 may be used independently (e.g., for sale) or in conjunction with other devices.

In this embodiment, the container 1 is a chip-scale rectangular structure and is used to contain a liquid sample S, which includes a plurality of biological particles P and a transfection substance T. However, the present invention is not limited thereto. For example, the number of biological particles P contained in the liquid sample S can be adjusted according to actual needs (e.g., at least one).

It should be noted that the liquid sample S can be a body fluid sample from an animal (e.g., blood, lymph, saliva, or urine), and the biological particles P can be specific types of cells or cell clusters, such as circulating tumor cells (CTCs), fetal nucleated red blood cells (FNRBCs), or bacteria. The transfectant T contains genetic material and can be at least one of ribonucleic acid (RNA), deoxyribonucleic acid (DNA), exosomes, liposomes, and viruses, but the present invention is not limited to these. For example, in other embodiments not shown in the present invention, the liquid sample S can also be a liquid sample from a plant.

The container 1 includes a light-sensing structure 11, a mating structure 12 spaced apart from the light-sensing structure 11, a plurality of frames 13 disposed between the light-sensing structure 11 and the mating structure 12, and a bonding layer 14 connecting the light-sensing structure 11 and the mating structure 12. At least one of the light-sensing structure 11 and the mating structure 12 is transparent. In this embodiment, the light-sensing structure 11 and the mating structure 12 are two parallel plate-like structures, with the distance between them being greater than the size of any of the biological particles P. However, the present invention is not limited to this.

More specifically, the photosensitive structure 11 includes a first substrate 111, a first electrode layer 112 formed on the first substrate 111, and a photoelectric layer 113 formed on the first substrate 111. In this embodiment, the first electrode layer 112 is formed on the bottom side of the first substrate 111, and the photoelectric layer 113 is formed on the top side of the first substrate 111. The photoelectric layer 113 includes a plurality of transistors 1131 arranged in a matrix, but the present invention is not limited thereto.

The mating structure 12 includes a second substrate 121 and a second electrode layer 122 formed on the second substrate 121, with the second electrode layer 122 facing the photosensitive structure 11 (e.g., the photoelectric layer 113). In this embodiment, the AC device 2 is electrically coupled to the first electrode layer 112 of the photosensitive structure 11 and the second electrode layer 122 of the mating structure 12, so that the photosensitive structure 11 can be illuminated by light emitted by the light capture device 3, forming a dielectrophoretic pattern F. The dielectrophoretic pattern F is then used to move at least one biological particle P or transfection substance T within the liquid sample S.

For example, the light capturing device 3 may include a camera 31 and a light source 32 associated with the camera 31. The light capturing device 3 can emit light from the light source 32 to illuminate the light sensing structure 11, so that the dielectrophoretic pattern F is formed on the light sensing structure 11.

The multiple frames 13 are sandwiched between the photoelectric layer 113 of the light-sensing structure 11 and the second electrode layer 122 of the mating structure 12. Since the structures of the multiple frames 13 are substantially identical in this embodiment, for ease of explanation, the following description will first focus on the structure of a single frame 13. However, the present invention is not limited to this. For example, in other embodiments not shown in the present invention, the structures of the multiple frames 13 may also differ slightly.

In this embodiment, the frame 13 has a mirror-symmetrical structure and includes two working sections 131 and two channel sections 132 extending from the two working sections 131. The two working sections 131 are spaced apart and facing each other, and the two channel sections 132 are also spaced apart and facing each other, but the present invention is not limited to this. For example, as shown in FIG4 , the frame 13 may also omit the two channel sections 132 according to actual needs.

More specifically, the two working sections 131 are formed at opposite ends with a first opening 1311 and a second opening 1312, which is smaller than the first opening 1311. In other words, the area between the two working sections 131 is connected to the outside via the first opening 1311 and the second opening 1312, respectively. Furthermore, the two channel sections 132 are connected to the portions of the two working sections 131 defining the second opening 1312, and each of the two channel sections 132 defines a third opening 1321, which is larger than the second opening 1312 and is distal from the second opening 1312. In this embodiment, the dimensions of the third opening 1321 are substantially equal to those of the second opening 1312, but the present invention is not limited thereto.

Furthermore, in this embodiment, the area between the two working sections 131 forms a receiving area 1313 and a neck region 1314 connected to the receiving area 1313. The first opening 1311 is defined at one end of the receiving area 1313, while the second opening 1312 is defined in the neck region 1314, which is distal to the first opening 1311. The receiving area 1313 has a width substantially the same as that of the first opening 1311, and the neck region 1314 is located at the other end of the receiving area 1313 and gradually tapers away from the receiving area 1313, thereby defining the second opening 1312. However, the present invention is not limited thereto.

It should be noted that at least one of the plurality of biological particles P is defined as a target biological particle P1, having a particle size larger than the second opening 1312 but smaller than the first opening 1311. Furthermore, the cell surface layer P1-1 of the target biological particle P1 has an initial permeability. For example, the cell surface layer P1-1 may be a cell membrane or a cell wall. Furthermore, the transfection substance T is smaller than the second opening 1312.

As shown in Figures 3, 5, and 6, the light-capturing device 3 can be used to form a first dielectrophoretic pattern F1 on the light-sensing structure 11. This first dielectrophoretic pattern F1 can be used to drive the target biological particle P1 through the first opening 1311 and into the space between the two working sections 131. In this embodiment, the light-capturing device 3 emits light onto the light-sensing structure 11 to form the first dielectrophoretic pattern F1, which is closed and surrounds the target biological particle P1. The first dielectrophoretic pattern F1 then drives the target biological particle P1 through the first opening 1311 and into the receiving area 1313.

Furthermore, when the target biological particle P1 is captured within the two working sections 131, the light capture device 3 can be used to form a second dielectrophoretic pattern F2 on the light-sensing structure 11. This second dielectrophoretic pattern F2 drives the transfection substance T through the second opening 1312 and into the space between the two working sections 131, thereby achieving a transfection operation. Accordingly, when the transfection substance T contains the virus, during the transfection operation, the target biological particle P1 is captured between the two working sections 131 and has the initial permeability.

It should be noted that when the target biological particle P1 is captured between the two working sections 131, the light-capturing device 3 can be used to form a third dielectrophoretic pattern F3 on the light-sensing structure 11. This third dielectrophoretic pattern F3 maintains the target biological particle P1 between the two working sections 131, thereby facilitating the transfection process. Furthermore, the specific shapes of the second and third dielectrophoretic patterns F2 and F3 can be adjusted based on actual needs.

In addition, although the position of the target biological particle P1 is restricted by the third dielectrophoretic pattern F3 in this embodiment, in other embodiments not shown in the present invention, the target biological particle P1 may also be controlled by the pressure of the liquid sample S, thereby restricting the target biological particle P1 to a specific position.

Furthermore, the triggering device 4 is disposed relative to the frame 13 and is configured to trigger the cell surface layer P1-1 of the target biological particle P1 located between the two working sections 131, thereby increasing the cell surface layer P1-1 to a predetermined permeability greater than the initial permeability. It should be noted that the triggering device 4 can employ electrical or light energy to temporarily increase the permeability of the cell surface layer P1-1; for example, electroporation, laser transfection, or light injection. However, for ease of understanding, the triggering device 4 is described in this embodiment using the electroporation method, but the present invention is not limited thereto.

Specifically, the triggering device 4 includes two electrode pads 41 and a power source 42 electrically coupled to the two electrode pads 41. In this embodiment, the power source 42 is illustrated as a direct current (DC) power source, with the two electrode pads 41 serving as a positive electrode and a negative electrode, respectively (e.g., the two electrode pads 41 are located within the receiving area 1313 and disposed on the inner walls of the two working sections 131, respectively). However, the present invention is not limited thereto. For example, in other embodiments not shown, the power source 42 may also employ an alternating current (AC) power source, depending on actual needs.

When the target biological particle P1 is captured in the containing area 1313, the power source 42 can drive the two electrode pads 41 to apply an electric field (e.g., a short-term high-intensity electric field) to at least one of the target biological particles P1, so that the target biological particle P1 forms an electroporation, thereby making the cell surface layer P1-1 have the predetermined permeability.

Accordingly, when the transfection substance T includes at least one of the RNA, the DNA, the exosome, and the liposome, during the implementation of the transfection operation, the target biological particle P1 is captured between the two working sections 131 and passes through the triggering device 4 to have the predetermined permeability.

As described above, in this embodiment, the non-contact biological particle processing device 100 cooperates with the frame 13 and the trigger device 4 and is matched with the light sensing structure 11 of the container 1, so that the frame 13 can be used to locate the target biological particle P1, and the trigger device 4 can increase the permeability of the cell surface layer P1-1 of the target biological particle as needed, thereby facilitating the precise operation of the target biological particle P1.

[Example 2]

Please refer to FIG7 , which is a second embodiment of the present invention. Since this embodiment is similar to the first embodiment, the similarities between the two embodiments will not be described again. The difference between this embodiment and the first embodiment mainly lies in the triggering device 4.

In this embodiment, the two electrode pads 41 are located in the neck region 1314 and are disposed on the inner walls of the two working sections 131. When the target biological particle P1 is captured in the neck region 1314, the power source 42 drives the two electrode pads 41 to apply an electric field (e.g., a short, high-intensity electric field) to the target biological particle P1, thereby causing electroporation in the target biological particle P1, thereby achieving the predetermined permeability of the cell surface layer P1-1.

Accordingly, the light capture device 3 can confine the target biological particle P1 to the neck region 1314 using the third dielectrophoretic pattern F3, and the cell surface layer P1-1 portion of the target biological particle P1 adjacent to the second opening 1312 can be temporarily opened by the electroporation, so that the light capture device 3 can move the transfection substance T through the second opening 1312 to the neck region 1314 using the second dielectrophoretic pattern F2, thereby facilitating a more precise transfection operation between the target biological particle P1 and the transfection substance T.

[Example 3]

Please refer to Figures 8 to 10, which are embodiment 3 of the present invention. Since this embodiment is similar to the above-mentioned embodiments 1 and 2, the similarities between the above-mentioned embodiments will not be repeated. The difference between this embodiment and the above-mentioned embodiments 1 and 2 mainly lies in the operation of the trigger device 4.

In this embodiment, the triggering device 4 can be used to trigger the cell surface layer P1-1 of the target biological particle P1 located between the two working sections 131, so that the cell surface layer P1-1 has a predetermined permeability greater than the initial permeability, thereby causing the target biological particle P1 to proliferate an exosome T1 that passes through the cell surface layer P1-1.

When the target biological particle P1 is captured between the two working sections 131 and the exosomes T1 proliferate, the light capture device 3 can be used to form a second dielectrophoretic pattern F2 on the light sensing structure 11, so that the exosomes T1 are driven by the second dielectrophoretic pattern F2 to pass through the second opening 1312 between the two working sections 131 and leave the two working sections 131, thereby achieving a purification operation.

[Technical Effects of the Embodiments of the Present Invention]

In summary, the non-contact biological particle processing apparatus disclosed in the embodiments of the present invention utilizes the frame and the triggering device to cooperate with each other and be matched with the light-sensing structure of the container device, so that the frame can be used to locate the target biological particles. Furthermore, the triggering device can increase the permeability of the cell surface layer of the target biological particles as needed, thereby facilitating the precise processing of the target biological particles (such as the transfection process or the purification process).

The contents disclosed above are merely preferred feasible embodiments of the present invention and do not limit the patent scope of the present invention. Therefore, any equivalent technical changes made by using the contents of the description and drawings of the present invention are included in the patent scope of the present invention.

100: Non-contact biological particle processing device 1: Container 11: Photosensitive structure 111: First substrate 112: First electrode layer 113: Photoelectric layer 1131: Transistor 12: Coupling structure 121: Second substrate 122: Second electrode layer 13: Frame 131: Working section 1311: First opening 1312: Second opening 1313: Receiving area 1314: Neck section 132: Channel section 1321: Third opening 14: Laminating layer 2: AC device 3: Light capture device 31: Camera 32: Light source 4: Triggering device 41: Electrode pad 42: Power supply S: Liquid specimen P: Bioparticle P1: Target bioparticle P1-1: Cell surface layer T: Transfected substance T1: Exosomes F: Dielectrophoresis pattern F1: First dielectrophoresis pattern F2: Second dielectrophoresis pattern F3: Third dielectrophoresis pattern

FIG1 is a schematic perspective view of a non-contact biological particle processing apparatus according to a first embodiment of the present invention.

FIG2 is a schematic plan cross-sectional view of the non-contact biological particle processing apparatus according to the first embodiment of the present invention.

FIG3 is a schematic three-dimensional cross-sectional view of the non-contact biological particle processing apparatus according to the first embodiment of the present invention.

FIG4 is a cross-sectional diagram of another embodiment of FIG2.

FIG5 is a cross-sectional view of the subsequent operation of FIG2.

FIG6 is a cross-sectional diagram illustrating the subsequent operation of FIG5.

FIG7 is a schematic cross-sectional view of a non-contact biological particle processing apparatus according to a second embodiment of the present invention.

FIG8 is a schematic cross-sectional view of a non-contact biological particle processing apparatus according to a third embodiment of the present invention.

FIG9 is a schematic cross-sectional view of the subsequent operation of FIG8.

FIG10 is a schematic cross-sectional view of the subsequent operation of FIG9.

100: Non-contact biological particle treatment equipment

1: Storage device

11: Light sensing structure

111: First substrate

112: First electrode layer

113: Photoelectric layer

1131: Transistor

12: Coordination structure

121: Second substrate

122: Second electrode layer

13: Framework

14: Lamination layer

2: AC device

3: Light Capture Device

31: Camera

32: Light Source

4: Trigger device

41: Electrode pad

42: Power supply

F: Dielectrophoretic pattern

Claims (15)

A non-contact biological particle processing apparatus comprises: A container for containing a liquid sample containing a plurality of biological particles and a transfection substance, wherein the container comprises: A photosensitive structure comprising a first substrate, a first electrode layer formed on the first substrate, and a photoelectrolyte layer formed on the first substrate; A matching structure spaced apart from the photosensitive structure; wherein at least one of the photosensitive structure and the matching structure is transparent, and the matching structure comprises a second substrate and a second electrode layer formed on the second substrate, wherein the second electrode layer faces the photosensitive structure; and A frame disposed between the light-sensing structure and the mating structure; wherein the frame has two working sections facing each other, with a first opening and a second opening smaller than the first opening formed at opposite ends thereof; wherein at least one of the plurality of biological particles is defined as a target biological particle having a particle size larger than the second opening, and the cell surface layer of the target biological particle has an initial permeability; A light-capturing device facing the containing device; wherein the light-capturing device is configured to form a first dielectrophoretic pattern on the light-sensing structure, thereby driving the target biological particle through the first opening and into the space between the two working sections via the first dielectrophoretic pattern; and A triggering device is provided corresponding to the frame; the triggering device is configured to trigger the cell surface layer of the target biological particle located between the two working sections using electrical energy or light energy, thereby causing the cell surface layer to have a predetermined permeability greater than the initial permeability. When the target biological particle is captured between the two working sections, the light capture device is configured to form a second dielectrophoretic pattern on the light-sensing structure, thereby driving the transfection substance through the second opening and into the space between the two working sections via the second dielectrophoretic pattern to achieve a transfection operation. The non-contact biological particle processing apparatus of claim 1, wherein the region between the two working sections comprises: a containment region having the first opening defined at one end; and a neck region located at the other end of the containment region; wherein the neck region tapers away from the containment region and defines the second opening distal from the first opening. The non-contact biological particle processing apparatus of claim 2, wherein the triggering device comprises: two electrode pads located in the containment area and disposed on the inner walls of the two working sections, respectively; and a power source electrically coupled to the two electrode pads; wherein, when the target biological particles are captured in the containment area, the power source drives the two electrode pads to apply an electric field to the target biological particles, thereby causing electroporation of the target biological particles and thereby causing the cell surface layer to have the predetermined permeability. The non-contact biological particle processing apparatus of claim 2, wherein the triggering device comprises: two electrode pads located in the neck region and disposed on the inner walls of the two working sections, respectively; and a power source electrically coupled to the two electrode pads; wherein, when the target biological particle is captured in the neck region, the power source drives the two electrode pads to apply an electric field to the target biological particle, thereby causing electroporation of the target biological particle, thereby causing the cell surface layer to have the predetermined permeability. A non-contact biological particle processing device as described in claim 1, wherein the frame includes two channel sections, and the two channel sections are respectively connected to the two working section parts defining the second opening; wherein the two channel sections define a third opening far away from the second opening, which is larger than the second opening. A non-contact biological particle processing device as described in claim 1, wherein, when the target biological particles are captured between the two working sections, the light capturing device can be used to form a third dielectrophoretic pattern on the light sensing structure to maintain the target biological particles between the two working sections through the third dielectrophoretic pattern. The non-contact biological particle processing apparatus according to claim 1, wherein the transfection substance comprises at least one of ribonucleic acid (RNA), deoxyribonucleic acid (DNA), exosome, liposome, and virus. The non-contact biological particle processing apparatus as described in claim 1, wherein the transfection substance comprises at least one of RNA, DNA, exosomes, and liposomes, and during the implementation of the transfection operation, the target biological particles are captured between the two working sections and have the predetermined permeability. The non-contact biological particle processing device as described in claim 1, wherein the transfection substance contains viruses, and during the implementation of the transfection operation, the target biological particles are captured between the two working sections and have the initial permeability. A non-contact biological particle processing apparatus comprises: A container for containing a liquid sample containing a plurality of biological particles, the container comprising: A photosensitive structure comprising a first substrate, a first electrode layer formed on the first substrate, and a photoelectrolyte layer formed on the first substrate; A mating structure spaced from the photosensitive structure; wherein at least one of the photosensitive structure and the mating structure is transparent, and the mating structure comprises a second substrate and a second electrode layer formed on the second substrate, with the second electrode layer facing the photosensitive structure; and A frame disposed between the light-sensing structure and the mating structure; wherein the frame has two working sections facing each other, with a first opening and a second opening smaller than the first opening formed at opposite ends thereof; wherein at least one of the plurality of biological particles is defined as a target biological particle having a particle size larger than the second opening, and the cell surface layer of the target biological particle has an initial permeability; A light-capturing device facing the containing device; wherein the light-capturing device is configured to form a first dielectrophoretic pattern on the light-sensing structure, thereby driving the target biological particle through the first opening and into the space between the two working sections via the first dielectrophoretic pattern; and A triggering device is provided corresponding to the frame. The triggering device is configured to trigger the cell surface layer of the target biological particle located between the two working sections using electrical or optical energy, thereby increasing the cell surface layer to a predetermined permeability greater than the initial permeability, thereby causing the target biological particle to proliferate an exosome that passes through the cell surface layer. When the target biological particle is captured between the two working sections and the exosome proliferates, the light capture device is configured to form a second dielectrophoretic pattern on the light-sensing structure. The second dielectrophoretic pattern drives the exosome to pass through the second opening between the two working sections and exit the two working sections, thereby achieving a purification operation. The non-contact biological particle processing apparatus of claim 10, wherein the region between the two working sections comprises: a containment region having the first opening defined at one end; and a neck region located at the other end of the containment region; wherein the neck region tapers away from the containment region and defines the second opening distal from the first opening. The non-contact biological particle processing apparatus of claim 11, wherein the triggering device comprises: two electrode pads located in the containment area and disposed on the inner walls of the two working sections, respectively; and a power source electrically coupled to the two electrode pads; wherein, when the target biological particles are captured in the containment area, the power source drives the two electrode pads to apply an electric field to the target biological particles, thereby causing electroporation of the target biological particles and thereby causing the cell surface layer to have the predetermined permeability. The non-contact biological particle processing apparatus of claim 11, wherein the triggering device comprises: two electrode pads located in the neck region and disposed on the inner walls of the two working sections, respectively; and a power source electrically coupled to the two electrode pads; wherein, when the target biological particle is captured in the neck region, the power source drives the two electrode pads to apply an electric field to the target biological particle, thereby causing electroporation of the target biological particle, thereby causing the cell surface layer to have the predetermined permeability. A non-contact biological particle processing device as described in claim 10, wherein the frame includes two channel sections, and the two channel sections are respectively connected to the two working section parts defining the second opening; wherein the two channel sections define a third opening far away from the second opening, which is larger than the second opening. A non-contact biological particle processing apparatus comprises: A container for containing a liquid sample containing a plurality of biological particles, the container comprising: A photosensitive structure comprising a first substrate, a first electrode layer formed on the first substrate, and a photoelectrolyte layer formed on the first substrate; A mating structure spaced from the photosensitive structure; wherein at least one of the photosensitive structure and the mating structure is transparent, and the mating structure comprises a second substrate and a second electrode layer formed on the second substrate, with the second electrode layer facing the photosensitive structure; and A frame disposed between the light-sensing structure and the mating structure; wherein the frame has two working sections facing each other, each having a first opening and a second opening smaller than the first opening formed at opposite ends thereof; wherein at least one of the plurality of biological particles is defined as a target biological particle having a particle size larger than the second opening, and the cell surface layer of the target biological particle has an initial permeability; and a triggering device disposed corresponding to the frame; wherein, when the target biological particle passes through the first opening and enters between the two working sections, the triggering device is configured to trigger the cell surface layer of the target biological particle using electrical energy or light energy, thereby causing the cell surface layer to have a predetermined permeability greater than the initial permeability.