TWI890642B - Non-invasive method for estimating blood glucose and the device thereof - Google Patents

Abstract

A non-invasive device for estimating blood glucose includes a substrate, on which a light source, a receiver, a wall, a power source, a current controller, a control unit, and a calculating unit are provided. The light source emits a detecting light to the skin of a subject, while the current controller changes the current provided to the light source to make the light source emit the detecting lights with different intensities that makes the detect lights go to different depths under the skin. The receiver senses the reflected lights of the detecting lights and transmits them to the calculating unit. The calculating unit processes the reflected lights to obtain an estimated value of blood glucose.

Description

Non-invasive blood glucose measurement method and device

The present invention relates to a non-invasive blood glucose measurement method, and more particularly to a non-invasive blood glucose measurement method that obtains blood glucose information by probing different subcutaneous depths.

Diabetes is a chronic disease that develops when the pancreas doesn't produce enough insulin or when the body can't effectively use the insulin it produces. Insulin is a hormone that regulates blood sugar. Hyperglycemia, or elevated blood sugar, is a common consequence of uncontrolled diabetes. Over time, it can cause severe damage to many of the body's systems, particularly the nervous and blood vessels, and can lead to heart disease, kidney failure, blindness, and amputation.

The World Health Organization (WHO) released its first Global Report on Diabetes on April 6, 2016. The report, which indicated that the number of people with diabetes worldwide has exceeded 400 million, a fourfold increase since 1980, sparked widespread concern. The WHO predicts that diabetes will become the seventh leading cause of death worldwide by 2030.

Generally speaking, diabetic patients often need to self-test their blood sugar levels. This allows them to immediately determine their blood sugar levels, control their diet, and maintain their blood sugar levels. By recording these data regularly, they can provide medical staff with reference during consultations to provide appropriate treatment.

Early blood sugar tests used to measure blood sugar levels using urine test strips, which determined blood sugar levels through a colorimetric method. Currently, testing is often done by finger prick, where a blood glucose meter reads the blood sample on the test strip and calculates the blood sugar concentration. However, this method can cause pain and discomfort, and puts patients at risk of infectious diseases. It also prevents constant monitoring of blood sugar levels, leading to the development of non-invasive blood sugar measurement devices.

Current non-invasive blood glucose measurement devices are known to have low accuracy. This is because a single wavelength of a light-emitting element does not significantly vary the parameters measured across different individuals. Therefore, many use more than one set of light-emitting elements to generate light of different wavelengths, thereby increasing the variation in measured parameters. Significant variation translates to improved accuracy, but using more than one set of light-emitting elements increases the size and weight of the device, making it inconvenient to wear and carry. This reduces cost-effectiveness, increases costs, and increases product assembly time.

The object of the present invention is to provide a non-invasive blood glucose measurement method and device, which can improve measurement accuracy and is suitable for small-volume blood glucose measurement devices.

To achieve the aforementioned purpose, the present invention provides a non-invasive blood glucose measurement method comprising the following steps:

Step 1: Generate a detection light having a predetermined wavelength and a predetermined intensity to illuminate the skin of a subject, receive reflected light from the detection light, perform predetermined processing on the reflected light, and record it as a detection signal;

Step 2: Generating a detection light having the same wavelength as the detection light in step 1 but a different intensity to illuminate the subject's skin, then receiving reflected light from the detection light, performing predetermined processing on the reflected light, and recording it as a detection signal;

Step 3: Repeat step 2 n times to obtain n detection signals; where n is a positive integer greater than or equal to 0; and

Step 4: Substitute the detection signals into a prediction equation and obtain an estimated value after calculation.

In one embodiment, the wavelength of the detection light is between 800 and 3000 nm.

In one embodiment, the luminous flux of the light source generating the detection lights is between 1 and 100 mW.

In one embodiment, the preferred range of n is 1≤n≤16.

In one embodiment, the non-invasive blood glucose measurement method further includes the following steps: repeatedly executing steps 1 to 4 at different times to obtain a plurality of estimated values; and displaying the estimated values in chronological order.

In one embodiment, a calibration procedure is further included; the calibration procedure includes the following steps: 1. Executing steps 1 to 4 to obtain an estimated value, and measuring the subject using a known blood glucose measurement device to obtain a measured value; and obtaining a conversion function using the correspondence between the estimated value and the measured value; wherein, the estimated value obtained according to the measurement method of the present invention is substituted into the conversion function to obtain an actual value.

In one embodiment, the calibration procedure further includes the following steps after step 1: repeating step 1 several times to obtain a plurality of estimated values and a plurality of measured values; wherein the conversion function is calculated using the estimated values and the measured values.

The present invention further provides another non-invasive blood glucose measurement method, comprising the following steps:

Step 1: Providing a first light source to generate a first detection light having a predetermined wavelength and a predetermined light intensity to illuminate the skin of a subject, then receiving reflected light of the first detection light, performing predetermined processing on the reflected light, and recording the reflected light as a detection signal;

Step 2: Changing the voltage supplied to the first light source to generate a first detection light having different light intensities to illuminate the subject's skin, then receiving reflected light from the first detection light, performing predetermined processing on the reflected light, and recording the reflected light as a detection signal;

Step 3: Repeat step 2 n times to obtain n detection signals; where n is a positive integer greater than or equal to 0;

Step 4: Providing a second light source to generate a second detection light having a predetermined wavelength and a predetermined intensity to illuminate the subject's skin, then receiving reflected light from the second detection light, performing predetermined processing on the reflected light, and recording the reflected light as a detection signal; wherein the wavelength of the first detection light is different from the wavelength of the second detection light;

Step 5: Changing the voltage supplied to the second light source to generate a second detection light having a different light intensity to illuminate the subject's skin, then receiving reflected light from the second detection light, performing a predetermined process on the reflected light, and recording the reflected light as a detection signal;

Step 6: Repeat step 5 n times to obtain n detection signals; where n is a positive integer greater than or equal to 0; and

Step 7: Substitute the detection signals obtained by the first light source and the second light source into a prediction equation to obtain an estimated value after calculation.

In one embodiment, the wavelength of the first detection light is between 800 and 1300 nm; the wavelength of the second detection light is between 1300 and 3000 nm.

In one embodiment, the preferred range of n is 1≤n≤16.

Furthermore, the present invention provides a non-invasive blood glucose measuring device, comprising: a substrate having a predetermined circuit layout; a first light source disposed on the substrate and electrically connected to the circuit layout, for generating a first detection light having a predetermined wavelength; a receiver disposed on the substrate and electrically connected to the circuit layout, for receiving reflected light of the first detection light generated by the first light source; a power source disposed on the substrate and electrically connected to the circuit layout; A circuit layout is provided for providing power; a current regulator is disposed on the substrate and electrically connected to the circuit layout, and is used to change the current provided to the first light source, thereby changing the light intensity of the first detection light generated by the first light source; and a calculation unit is disposed on the substrate and electrically connected to the circuit layout, and is used to receive the reflected light detected by the receiver, convert the detected light into a detection signal, and substitute the detected signal into a prediction equation to obtain an estimated value.

In one embodiment, the first light source and the receiver are located on the same side of the substrate, and a partition is disposed on the substrate between the first light source and the receiver to prevent the first detection light from directly irradiating the receiver.

In one embodiment, the system further includes a second light source disposed on the substrate and electrically connected to the circuit layout, wherein the second light source generates a second detection light, and the first detection light and the second detection light have different wavelengths. The current regulator can change the current provided to the second light source so that the second detection light has different light intensities. The receiver detects the reflected light of the first detection light and the second detection light, and then converts the reflected light of the first detection light and the second detection light into detection signals by the calculation unit. The detection signals are then substituted into a prediction equation to obtain an estimated value.

In one embodiment, the first light source, the second light source, and the receiver are located on the same side of the substrate, and the substrate is provided with a partition wall. The first light source and the second light source are located on one side of the partition wall, and the receiver is located on the other side of the partition wall to prevent the first detection light and the second detection light from directly irradiating the receiver.

The following is a detailed description of preferred embodiments based on the objectives, effects, and structural configurations disclosed in the present invention, along with accompanying drawings.

FIG1 is a schematic diagram of a non-invasive blood glucose measurement device 1 according to a first preferred embodiment of the present invention. The device comprises a substrate 10 on which are mounted a light source 12, a receiver 14, a partition 16, a power source 18, a current regulator 20, a control unit 22, and a calculation unit 24. The substrate 10 is provided with a circuit layout (not shown), to which the aforementioned components are electrically connected to establish predetermined electrical connections.

In this embodiment, the light source 12 is a light-emitting diode (LED) that generates detection light of a predetermined wavelength. The wavelength of the detection light is between 800 and 3000 nm, preferably between 1000 and 2000 nm. The current regulator 20 is disposed between the power supply 18 and the light source 12. Controlled by the control unit 22, it varies the current supplied by the power supply 18 to the light source 12, thereby causing the light source 12 to generate detection light of varying intensities. In this embodiment, the current regulator 20 adjusts the luminous flux of the light source 12 between 1 and 100 mW, preferably between 10 and 50 mW.

The receiver 14 is disposed on the substrate 10, on the same side as the light source 12, and is configured to receive the reflected light from the detection light of the light source 12. The partition 16 is located between the light source 12 and the receiver 14 to prevent the light from the light source 12 from directly striking the receiver 14, thereby ensuring that the optical signal received by the receiver 14 is the reflected light from the light source 12 after it enters the skin S.

The computing unit 24 is connected to the receiver 14 to record, process, and calculate the optical signal received by the receiver 14. The control unit 22 controls the aforementioned components to perform predetermined actions. The power supply 18 provides the necessary power.

The non-invasive blood glucose measuring device 1 of the first preferred embodiment of the present invention is a wearable device, such as a watch, earphones, glasses, or a ring, which is worn at a predetermined position on the human body with the light source 12 and the receiver 14 facing the skin S of the subject.

Referring to FIG. 2 , measuring blood glucose using the non-invasive blood glucose measuring device 1 described above includes the following steps:

Step 1: The light source 12 generates a detection light with a predetermined wavelength to illuminate the skin S. The reflected light of the detection light is then received by the receiver 14. The calculation unit 24 then performs predetermined processing and records it as a detection signal.

According to academic reports, blood viscosity is significantly correlated with blood sugar fluctuations. When the detection light enters the skin (S), it is affected by the blood conditions in the blood vessels beneath the skin, such as blood concentration and flow rate. This causes changes in the properties of the reflected light, such as wavelength and intensity, which are then used to estimate the subject's blood sugar status.

In this embodiment, the receiver 14 is a photodiode, which generates an excitation current after being excited by the reflected light of the detection light. The excitation current is amplified by an amplifier, filtered by a filter to remove noise, and then converted into a digital signal by an analog-to-digital converter to generate the detection signal.

Step 2: Activate the current regulator 20 to change the current supplied to the light source 12, causing the light source 12 to generate detection light of the same wavelength but different light intensities to illuminate the skin S. The receiver 14 then receives the reflected light of the detection light. The calculation unit 24 then performs predetermined processing and records it as a detection signal.

Step 3: Repeat step 2 n times to obtain n detection signals.

Because the location of blood vessels varies among subjects, the detection light generated in step 1 may not reach the blood vessels. Therefore, the detection signal generated by the reflected light cannot reflect the blood status within the vessels. The present invention varies the current supplied to the light source 12 to change the intensity of the detection light. This allows each detection light beam to penetrate the human body at different depths, ensuring that the reflected light contains a detection signal that truly reflects the blood status, resulting in more accurate detection results.

The number of repetitions, n, in step 3 is a positive integer greater than or equal to 0. In other words, at least two detection signals must be obtained to facilitate the next step. In one embodiment, n is in the range of 1≤n≤256, and preferably in the range of 1≤n≤16.

Step 4: The calculation unit 24 substitutes the detection signals into a prediction equation and obtains an estimated value after calculation.

In this embodiment, the calculation unit 24 is disposed within the blood glucose measuring device 1. In another embodiment, the blood glucose measuring device 1 is connected to an external device (not shown), such as a smartphone, computer, or tablet, via a wireless connection such as Bluetooth, WiFi, or Zegbee, or a wired connection. The detection signals are transmitted to the external device for processing and calculation to obtain the estimated value.

Step 5: Repeat steps 1 to 4 at different times to obtain a plurality of estimated values, and display the estimated values on the display of the blood glucose monitoring device 1 or the external device using graphics or numbers in chronological order to show the blood glucose change status.

The estimated values obtained through the above steps can only show the blood sugar fluctuation status of the subject. To obtain the actual blood sugar value, the following calibration process is required:

Referring to FIG. 3 , the blood glucose measuring device 1 of the present invention performs steps 1 through 4 to obtain an estimated blood glucose value. A conventional blood glucose measuring device (not shown, such as a blood sampling device) is then used to measure the subject's actual blood glucose value. This actual value is then input into the blood glucose measuring device 1 to obtain a pair of corresponding estimated and actual values.

Repeat the above steps several times to obtain a plurality of estimated values and the measured values corresponding to the estimated values.

A conversion function is obtained by using the correspondence between the estimated values and the measured values.

Thereafter, the estimated value obtained by measuring the blood glucose level using the blood glucose measuring device 1 of the present invention can be used to generate an actual blood glucose value using the conversion function.

Referring to FIG. 4 , the non-invasive blood glucose measuring device 2 provided by the second preferred embodiment of the present invention includes: a substrate 10 on which are disposed a first light source 26 and a second light source 28, a receiver 14, a partition 16, a power source 18, a current regulator 20, a control unit 22, and a calculation unit 24. The substrate 10 is provided with a circuit layout (not shown), to which the aforementioned components are electrically connected to generate a predetermined circuit. The main components of the blood glucose measuring device 2 of the second preferred embodiment are the same as those of the first preferred embodiment, with the only difference being that the second preferred embodiment provides two light sources that generate detection light of different wavelengths. The first light source 26 and the second light source 28 are located on one side of the partition 16, and the receiver 14 is located on the other side. In this embodiment, the wavelength of the first detection light generated by the first light source 26 is between 800 and 1300 nm, preferably between 1300 and 3000 nm; the wavelength of the second detection light generated by the second light source 28 is between 1000 and 1300 nm, preferably between 1300 and 2000 nm.

Referring to FIG. 4 , the non-invasive blood glucose measurement device according to the second preferred embodiment of the present invention is used to perform blood glucose measurement, which includes the following steps:

Step 1: The first light source 26 generates a first detection light with a predetermined wavelength to illuminate the skin S of a subject. The reflected light of the first detection light is then received by the receiver 14. The reflected light is then processed by the calculation unit 24 and recorded as a detection signal.

Step 2: Activate the current regulator 20 to change the current supplied to the first light source 26, causing the first light source 26 to generate first detection light of varying intensities to illuminate the skin S. The receiver 14 then receives the reflected light of the first detection light. The calculation unit 24 then performs predetermined processing and records the reflected light as a detection signal.

Step 3: Repeat step 2 n times to obtain n detection signals; where n is a positive integer greater than or equal to 0.

Step 4: The second light source 28 generates a second detection light beam of a predetermined wavelength, which is then irradiated onto the subject's skin S. The reflected light of the second detection light is then received by the receiver 14. The reflected light is then processed by the computing unit 24 and recorded as a detection signal. The wavelength of the first detection light is different from that of the second detection light.

Step 5: Activate the current regulator 20 to change the current supplied to the second light source 28, causing the second light source 28 to generate a second detection light of varying intensities to illuminate the skin S. The receiver 14 then receives the reflected light of the detection light. The calculation unit 24 then performs predetermined processing and records the reflected light as a detection signal.

Step 6: Repeat step 5 n times to obtain n detection signals; where n is a positive integer greater than or equal to 0.

Step 7: The calculation unit 24 substitutes the detection signals obtained by the first light source 26 and the second light source 28 into a prediction equation to obtain an estimated value after calculation.

Step 8: Repeat steps 1 to 7 at different times to obtain a plurality of estimated values, and display the estimated values on the display of the blood glucose monitoring device 2 or the external device using graphics or numbers in chronological order to show the blood glucose change status.

The second preferred embodiment differs from the first preferred embodiment only in that the second preferred embodiment provides two light sources, namely, a first light source 26 and a second light source 28, each providing light of different wavelengths. As is well known, longer wavelengths of light have greater penetrating power. By varying the current supplied to the first and second light sources 26, 28 through the current regulator 20, the detection light provided by the first and second light sources 26, 28 can reach different depths beneath the skin S due to their varying wavelengths and intensities, thereby improving measurement accuracy.

The purpose of this design is that due to the limited internal space of general wearable devices, the range of the current that can be changed by the current regulator 20 is also limited. By increasing the provision of illumination light of different wavelengths, the measurable depth is multiplied. It should be pointed out here that the second preferred embodiment uses two light sources as an example, but in actual implementation, the number of light sources can be 3, 4, 5 or more. In addition, the aforementioned embodiment is carried out in the order of first changing the light intensity of the first light source and then changing the light intensity of the second light source. Obviously, the first light source and the second light source can also be lit in sequence with the same current first, and then the first light source and the second light source can be lit in sequence after changing the current size. Or it can be carried out in a random manner to meet the inventive concept of the present invention.

Since the primary difference between the second preferred embodiment and the second preferred embodiment lies solely in the number of light sources, the remaining aspects not described, such as the calibration process, can be implemented using the same techniques as those described in the first preferred embodiment and will not be further elaborated upon here. Furthermore, while the present embodiment uses blood glucose measurement as an example, the principles of the device and method provided by this invention can also be used to measure relevant physiological data such as blood pressure, pulse, and blood oxygen levels.

According to the above-mentioned measuring devices 1 and 2 and their measuring methods, relatively accurate non-invasive measurement results can be achieved and can be applied to any small-volume measuring device.

The above embodiments are merely illustrative of the techniques and effects of the present invention and are not intended to limit the present invention. Any person skilled in the art may modify or alter the above embodiments without departing from the technical principles and spirit of the present invention. Therefore, the scope of protection of the present invention shall be as described in the patent application below.

1, 2: Blood glucose meter 10: Substrate 12: Light source 14: Receiver 16: Partition 18: Power supply 20: Current regulator 22: Control unit 24: Calculation unit 26: First light source 28: Second light source S: Skin

Figure 1 is a schematic diagram of the measuring device of the first preferred embodiment of the present invention. Figure 2 is a measurement flow chart of the first preferred embodiment of the present invention. Figure 3 is a flow chart of the calibration process of the first preferred embodiment of the present invention. Figure 4 is a schematic diagram of the measuring device of the second preferred embodiment of the present invention. Figure 5 is a measurement flow chart of the second preferred embodiment of the present invention.

1: Blood glucose monitoring device

10:Substrate

12: Light Source

14: Receiver

16: Partition

18: Power supply

20: Current Regulator

22: Control unit

24: Computing unit

S: Skin

Claims (20)

A non-invasive blood glucose measurement method comprises the following steps: Step 1: Generating a detection light having a predetermined wavelength and a predetermined intensity to illuminate the skin of a subject, then receiving reflected light from the detection light, performing predetermined processing on the reflected light, and recording the reflected light as a detection signal; Step 2: Generating a detection light having the same wavelength but a different intensity as the detection light from step 1 to illuminate the skin of the subject, then receiving reflected light from the detection light, performing predetermined processing on the reflected light, and recording the reflected light as a detection signal; Step 3: Repeating step 2 n times to obtain n detection signals; where n is a positive integer greater than or equal to 0; and Step 4: Substitute the detection signals into a prediction equation and obtain an estimated value after calculation. The non-invasive blood glucose measurement method as described in claim 1, wherein the wavelength of the detection light is between 800 and 3000 nm. The non-invasive blood glucose measurement method as described in claim 1, wherein the luminous flux of the light source generating the detection light is between 1 and 100 mW. The non-invasive blood glucose measurement method as described in claim 1, wherein the preferred range of n is 1≤n≤16. The non-invasive blood glucose measurement method as described in claim 1 further comprises the following steps: Repeating steps 1 to 4 of claim 1 at different times to obtain a plurality of estimated values; and Displaying the estimated values in chronological order. The non-invasive blood glucose measurement method of claim 1 further includes a calibration procedure; the calibration procedure comprises the following steps: 1. Executing steps 1 to 4 of claim 1 to obtain an estimated value, and measuring the subject using a known blood glucose measurement device to obtain a measured value; and 2. Utilizing the correspondence between the estimated value and the measured value to obtain a conversion function; Wherein, substituting the estimated value obtained according to the measurement method of claim 1 into the conversion function yields an actual value. The non-invasive blood glucose measurement method of claim 6, wherein after step 1 of the calibration procedure, the following step is further included: Repeating step 1 multiple times to obtain a plurality of estimated values and a plurality of measured values; wherein the conversion function of step 2 is calculated using the estimated values and the measured values. A non-invasive blood glucose measurement method comprises the following steps: Step 1: Providing a first light source to generate a first detection light having a predetermined wavelength and a predetermined intensity to illuminate the skin of a subject, then receiving reflected light from the first detection light, performing predetermined processing on the reflected light, and recording the reflected light as a detection signal; Step 2: Changing the voltage supplied to the first light source to generate first detection light having different intensities to illuminate the skin of the subject, then receiving reflected light from the first detection light, performing predetermined processing on the reflected light, and recording the reflected light as a detection signal; Step 3: Repeating step 2 n times to obtain n detection signals; where n is a positive integer greater than or equal to 0; Step 4: Providing a second light source to generate a second detection light having a predetermined wavelength and a predetermined intensity to illuminate the subject's skin, then receiving reflected light from the second detection light, performing predetermined processing on the reflected light, and recording it as a detection signal; wherein the wavelength of the first detection light is different from the wavelength of the second detection light; Step 5: Changing the voltage provided to the second light source to generate a second detection light having a different intensity to illuminate the subject's skin, then receiving reflected light from the second detection light, performing predetermined processing on the reflected light, and recording it as a detection signal; Step 6: Repeating Step 5 n times to obtain n detection signals; wherein n is a positive integer greater than or equal to 0; and Step 7: Substitute the detection signals obtained by the first light source and the second light source into a prediction equation to obtain an estimated value after calculation. The non-invasive blood glucose measurement method as described in claim 8, wherein the wavelength of the first detection light is between 800 and 1300 nm. The non-invasive blood glucose measurement method as described in claim 8, wherein the wavelength of the second detection light is between 1300 and 3000 nm. The non-invasive blood glucose measurement method as described in claim 8, wherein the preferred range of n is 1≤n≤16. The non-invasive blood glucose measurement method as described in claim 8 further comprises the following steps: Repeating steps 1 to 7 of claim 8 at different times to obtain a plurality of estimated values; and Displaying the estimated values in chronological order. The non-invasive blood glucose measurement method of claim 8 further includes a calibration procedure; the calibration procedure comprises the following steps: 1. Executing steps 1 to 7 of claim 8 to obtain an estimated value, and measuring the subject using a known blood glucose measurement device to obtain a measured value; and 2. Utilizing the correspondence between the estimated value and the measured value to obtain a conversion function; Wherein, substituting the estimated value obtained according to the measurement method of claim 8 into the conversion function yields an actual value. The non-invasive blood glucose measurement method of claim 13, wherein after step 1 of the calibration procedure, the method further comprises the following steps: Repeating step 1 multiple times to obtain a plurality of estimated values and a plurality of measured values; wherein the conversion function is calculated using the estimated values and the measured values. A non-invasive blood glucose measurement device comprises: a substrate having a predetermined circuit layout; a first light source disposed on the substrate and electrically connected to the circuit layout, for generating a first detection light having a predetermined wavelength; a receiver disposed on the substrate and electrically connected to the circuit layout, for receiving reflected light of the first detection light generated by the first light source; a power source disposed on the substrate and electrically connected to the circuit layout, for providing power; a current regulator disposed on the substrate and electrically connected to the circuit layout, for varying the current supplied to the first light source, thereby varying the intensity of the first detection light generated by the first light source; and A calculation unit, disposed on the substrate and electrically connected to the circuit layout, is configured to receive the reflected light detected by the receiver, convert it into a detection signal, and substitute it into a prediction equation to obtain an estimated value. The non-invasive blood glucose measuring device as described in claim 15, wherein the first light source and the receiver are located on the same side of the substrate, and the substrate is provided with a partition wall between the first light source and the receiver to prevent the first detection light from directly irradiating the receiver. The non-invasive blood glucose measuring device as described in claim 15, wherein the wavelength of the detection light generated by the first light source is between 800 and 1300 nm. The non-invasive blood glucose measuring device as described in claim 15, wherein under the action of the current regulator, the luminous flux of the first light source is between 1 and 100 mW. The non-invasive blood glucose measuring device as described in claim 15 further includes a second light source disposed on the substrate and electrically connected to the circuit layout, wherein the second light source generates a second detection light, and the first detection light and the second detection light have different wavelengths; the current regulator can change the current provided to the second light source so that the second detection light has different light intensities; the receiver detects the reflected light of the first detection light and the second detection light, and then converts the reflected light of the first detection light and the second detection light into detection signals by the calculation unit, and then substitutes the detection signals into a prediction equation to obtain an estimated value. A non-invasive blood glucose measuring device as described in claim 19, wherein the first light source, the second light source, and the receiver are located on the same side of the substrate, and the substrate is provided with a partition wall, the first light source and the second light source are located on one side of the partition wall, and the receiver is located on the other side of the partition wall to prevent the first detection light and the second detection light from directly irradiating the receiver.